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The UBE2L3 Ubiquitin Conjugating Enzyme: Interplay with Inflammasome Signalling and Bacterial Ubiquitin Ligases
The UBE2L3 ubiquitin conjugating enzyme: interplay with inflammasome signalling and bacterial ubiquitin ligases Matthew James George Eldridge 2018 Imperial College London Department of Medicine Submitted to Imperial College London for the degree of Doctor of Philosophy 1 Abstract Inflammasome-controlled immune responses such as IL-1β release and pyroptosis play key roles in antimicrobial immunity and are heavily implicated in multiple hereditary autoimmune diseases. Despite extensive knowledge of the mechanisms regulating inflammasome activation, many downstream responses remain poorly understood or uncharacterised. The cysteine protease caspase-1 is the executor of inflammasome responses, therefore identifying and characterising its substrates is vital for better understanding of inflammasome-mediated effector mechanisms. Using unbiased proteomics, the Shenoy grouped identified the ubiquitin conjugating enzyme UBE2L3 as a target of caspase-1. In this work, I have confirmed UBE2L3 as an indirect target of caspase-1 and characterised its role in inflammasomes-mediated immune responses. I show that UBE2L3 functions in the negative regulation of cellular pro-IL-1 via the ubiquitin- proteasome system. Following inflammatory stimuli, UBE2L3 assists in the ubiquitylation and degradation of newly produced pro-IL-1. However, in response to caspase-1 activation, UBE2L3 is itself targeted for degradation by the proteasome in a caspase-1-dependent manner, thereby liberating an additional pool of IL-1 which may be processed and released. UBE2L3 therefore acts a molecular rheostat, conferring caspase-1 an additional level of control over this potent cytokine, ensuring that it is efficiently secreted only in appropriate circumstances. These findings on UBE2L3 have implications for IL-1- driven pathology in hereditary fever syndromes, and autoinflammatory conditions associated with UBE2L3 polymorphisms. -
The Yeast-Hyphal Transition of Candida Albicans
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 15 June 2021 doi:10.20944/preprints202106.0386.v1 Review From Jekyll to Hyde: The yeast-hyphal transition of Candida albicans Eve Wai Ling Chow 1, Li Mei Pang 2 and Yue Wang 1,3* 1 Institute of Molecular and Cell Biology (IMCB), Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, Proteos, Singapore 138673; [email protected] 2 National Dental Research Institute Singapore (NDRIS), National Dental Centre Singapore, 5 Second Hospi- tal Ave, Singapore 168938; [email protected] 3 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 10 Medi- cal Drive, Singapore 117597 * Correspondence: [email protected] Abstract: Candida albicans is a major fungal pathogen of humans, accounting for 15% of nosocomial infections with an estimated attributable mortality of 47%. C. albicans is usually a benign member of the human microbiome in healthy people. Under constant exposure to highly dynamic environmen- tal cues in diverse host niches, C. albicans has successfully evolved to adapt to both commensal and pathogenic lifestyles. The ability of C. albicans to undergo a reversible morphological transition from yeast to filamentous forms is a well-established virulent trait. Over the past few decades, a signifi- cant amount of research has been carried out to understand the underlying regulatory mechanisms, signaling pathways, and transcription factors that govern the C. albicans yeast-to-hyphal transition. This review will summarize our current understanding of well-elucidated signal transduction path- ways that activate C. albicans hyphal morphogenesis in response to various environmental cues and the cell cycle machinery involved in the subsequent regulation and maintenance of hyphal morpho- genesis. -
Antiquariatsangebot Nr. 738 - 2
Versandantiquariat Robert A. Mueller Nachf. ∗ gegründet 1950 ∗ D-30916 Isernhagen Bothfelder Str. 11 Tel.:0511 / 61 40 70 Antiquariats-Angebot Nr. 738 Schach Schachzeitungen 30 % Sonderrabatt bis 31. Dezember 2006 Erhaltungszustands-Abkürzungen: 1)= Tadellos, 2)= gut erhalten, 3)= mit leichten Gebrauchsspuren, 4)= mit stärkeren Gebrauchsspuren, Na.= Name auf Titelblatt, (St.)= Stempel, U.= Unterstreichungen, (oJ.)= ohne Angabe des Erscheinungsjahres, Bibl.= Bibliotheksexemplar mit Stempel und Rückennummer (evtl. in Folie eingeschweißt). Falls Bestelltes nicht innerhalb 30 Tagen geliefert wurde, ist der Titel bereits verkauft. Sollte ein Titel kurzfristig (90 Tage) erneut angekauft werden können, wird dieser automatisch nachgeliefert. Benachrichtigung über verkaufte Titel nur auf Wunsch ! - 2006 - > Fax 0511 / 61 40 75 < > E-Mail [email protected] < Antiquariatsangebot Nr. 738 - 2 - 1) 31e hoogoven schaaktoernooi: De Grootmeestergroep. 192 S.Tab. Pp.-2) holländisch {3C129} 12,78 € 2) 32e Hoogoven Schaaktoernooi: Bulletin des Turniers. Januar 1970. 204 kopierte S.m.Tab. in einer Mappe zusammengeheftet. holländisch {3C1451} 10,23 € 3) 32e Hoogoven schaaktoernooi: Programm des Turniers. Wijk aan zee 1970. 22 S. m. Tab. Pp.-1/2) holländisch {3C2110} 5,11 € 4) 33e Hoogoven Schaaktoernooi: Programm des Turniers. Wijk aan zee 1971. 23 S. m. Tab. Pp.-1) holländisch {3C2098} 5,11 € 5) 40e Hoogovens Schaaktoernooi: Wijk aan Zee 19.-1./2.Febr.1978.persbulletin. Oce-Nederland BV. 14 S. Quart. geheftet. kt.-2) {AO646} 6,00 € 6) Schachmatoje obrosenije: Jahrgang 1980. Nr. 1-24. Schurnal komiteta po fisitscheskoi kulturje i sportu pri sowjetje ministrow SSSR. Isdajetsja s 1968 goda. Je Heft 32 S. m. zahl. Diagrammen. ungebunden - 2)24 Hefte. russisch Orriginalfassung {BR5073} 26,00 € 7) 7-es Internationales Schachturnier Italiens: Bulletin des Turniers. -
Identification of the Biosynthetic Gene Cluster of A-500359S In
The Journal of Antibiotics (2009) 62, 325–332 & 2009 Japan Antibiotics Research Association All rights reserved 0021-8820/09 $32.00 www.nature.com/ja ORIGINAL ARTICLE Identification of the biosynthetic gene cluster of A-500359s in Streptomyces griseus SANK60196 Masanori Funabashi1, Koichi Nonaka1, Chieko Yada1, Masahiko Hosobuchi1, Nobuhisa Masuda2, Tomoyuki Shibata3 and Steven G Van Lanen4 A-500359s, produced by Streptomyces griseus SANK60196, are inhibitors of bacterial phospho-N-acetylmuramyl-pentapeptide translocase. They are composed of three distinct moieties: a 5¢-carbamoyl uridine, an unsaturated hexuronic acid and an aminocaprolactam. Two contiguous cosmids covering a 65-kb region of DNA and encoding 38 open reading frames (ORFs) putatively involved in the biosynthesis of A-500359s were identified. Reverse transcriptase PCR showed that most of the 38 ORFs are highly expressed during A-500359s production, but mutants that do not produce A-500359s did not express these same ORFs. Furthermore, orf21, encoding a putative aminoglycoside 3¢-phosphotransferase, was heterologously expressed in Escherichia coli and Streptomyces albus, yielding strains having selective resistance against A-500359B, suggesting that ORF21 phosphorylates the unsaturated hexuronic acid as a mechanism of self-resistance to A-500359s. In total, the data suggest that the cloned region is involved in the resistance, regulation and biosynthesis of A-500359s. The Journal of Antibiotics (2009) 62, 325–332; doi:10.1038/ja.2009.38; published online 29 May 2009 Keywords: -
Phosphorylation and Mechanistic Regulation of a Novel Ikk
PHOSPHORYLATION AND MECHANISTIC REGULATION OF A NOVEL IKK SUBSTRATE, ITCH By JESSICA MARIE PEREZ Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy Dissertation Advisor: Dr. Derek W. Abbott Department of Pathology CASE WESTERN RESERVE UNIVERSITY January 2018 CASE WESTERN RESERVE UNIVERSITY SCHOOL OF GRADUATE STUDIES We hereby approve the thesis/dissertation of Jessica Marie Perez candidate for the Doctor of Philosophy degree*. (signed) Dr. George Dubyak (chair of the committee) Dr. Pamela Wearsch Dr. Theresa Pizarro Dr. Clive Hamlin Dr. Brian Cobb Dr. Derek Abbott (date) October 2, 2017 *We also certify that written approval has been obtained for any proprietary material contained therein. Table of Contents List of Tables ........................................................................................................ v List of Figures ..................................................................................................... vi Acknowledgements .......................................................................................... viii List of Abbreviations .......................................................................................... ix Abstract ............................................................................................................... xi Chapter 1: Introduction ....................................................................................... 1 1.1 Introduction ......................................................................................... -
Fbx Em Revista
FBX EM REVISTA Ano I Nº 1 Jul/Set 2009 FEDERAÇÃO BAHIANA DE XADREZ Fundada em 07/11/1960 homepage: http://www.fbx.com.br Email: [email protected] Revista da FBX nº 1 FBX Federação Bahiana de Xadrez Índice Calendário FBX 2009 3 Diretoria: 2008/2010 Próximos Eventos 4 Zonal Sergipe/Bahia Gambito Presidente: Open 4 José da Paixão Andrade Barbosa Semifinal do Campeonato Vice-Presidente: Baiano Absoluto de Xadrez Paulo Roberto Cruz Teixeira 2009 4 Vice-Presidente p/Interior: Final do Campeonato Baiano Ivan Abade dos Santos Absoluto de Xadrez 2009 4 1º Secretário: II IRT da FBX 5 Wilter Pereira Vieira Eventos Realizados no 2º Secretário: Primeiro Semestre de 2009 5 Cesídio Monteiro Rocha IX Torneio de Xadrez 1º Tesoureiro: Guararapes 5 Gilberto Oliveira e Silva Aberto do Brasil Cidade do 2ª Tesoureira: Salvador 8 Vânia Sampaio Botelho Classificação Final 9 Diretor Técnico: Partidas (Cinco Primeiras Jorge Henri Labussière Mesas) 10 Vice-Diretor Técnico: I IRT da FBX 17 Paulo Roberto Faraco de Lima Classificação Final 18 Diretor de Desenvolvimento de Xadrez Partidas (Cinco Primeiras Escolar: Rodadas) 18 Gileno Rodrigues do Amaral Grandes Mestres Brasileiros 25 Vice-Diretor de Desenvolvimento de João de Souza Mendes 25 Xadrez Escolar: Biografia 25 Walter Garrido Alvarez Partidas 26 Diretor de Desenvolvimento de Xadrez Mestres Internacionais Universitário: Baianos 34 Ramon Rodrigo Leão Ortega WMF Ruth Folk Cardoso 34 Diretor de Marketing: Dados Biográficos 34 Diogo Duarte Guimarães Partidas 36 Diretor de Informática: Problemas 40 José Romain -
Download the 2015 Abstract Booklet
WELCOME Dear CRINA Research Day Attendee, Thank you for joining us at the second annual CRINA Research Day. Last year at our inaugural event we welcomed over 300 attendees and featured more than 150 posters from many departments and faculties across campus. We are happy to announce that many of those attendees signed up to be members of CRINA, forming the core of our cancer research community. One year later, we continue to build our cancer research community by hosting a cancer-themed Research Day yet again. This year, we have given trainees an opportunity to both organize the program and present their work orally to our cancer research community at the University of Alberta. Further, we are emphasizing multidisciplinary collaborations that are so important for studying the complexities of cancer, by featuring team oral presentations. We hope that you continue to explore what the University of Alberta has to offer in the cancer research sphere and grow your network of collaborators through future CRINA Research Days. CRINA as an institute has a well-established reporting structure with operations committees and advisory boards. At our core, we continue to strengthen connections within our cancer research community by hosting events throughout the year such as seminars and symposia. Our leadership team is working on defining U of A cancer research strengths in terms of both, research brilliance and available equipment, with plans to build on this excellence to accelerate discovery and innovation. CRINA also represents the interests of its members as a unified voice on the provincial stage, working with AHS, AIHS and ACF. -
Structural and Functional Studies of Pseudomurein Peptide Ligases In
Copyright is owned by the Author of the thesis. Permission is given for a copy to be downloaded by an individual for the purpose of research and private study only. The thesis may not be reproduced elsewhere without the permission of the Author. ii Structural and functional studies of pseudomurein peptide ligases in methanogenic archaea A dissertation presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Biochemistry Massey University, Manawatu, New Zealand. Bishwa Prakash Subedi 2018 Abstract Prokaryotes are classified as Archaea and Bacteria in the tree of life and have several distinguishing characteristics, among which the cell wall is one of the most essential and early evolving. Cell walls serve a number of essential functions including protection against osmotic stress, maintenance of cell shape, reduction of lateral gene transfer, and protection from viruses. The cell walls in Bacteria are predominantly comprised of peptidoglycan (murein) whereas Archaea contain a wide range of cell wall types, none of them being murein. However, methanogens of the order Methanobacteriales and Methanopyrales contain pseudomurein that shares an overall architectural structure similar to that of murein with a glycan backbone that is cross-linked by a peptide. Understanding the enzymatic steps for pseudomurein pentapeptide biosynthesis and structural information of these enzymes, could be key to resolving the evolutionary history of cell wall synthesis and was the focus of this project. Analysis of the sequences and gene clusters of the murein peptide ligase genes suggested that analogous putative pseudomurein peptide ligases exist in methanogens. Moreover, the structures of two pseudomurein peptide ligases, pMurE and pMurC, the first of any archaeal peptide ligase, have been determined and their structural homology with bacterial murein ligase MurE and MurC, respectively, was analysed. -
Download Product Insert (PDF)
PRODUCT INFORMATION D-Glutamic Acid Item No. 31503 CAS Registry No.: 6893-26-1 Synonyms: Glutamic Acid, NSC 77686 O O MF: C5H9NO4 FW: 147.1 HO OH ≥95% Purity: NH Supplied as: A crystalline solid 2 Storage: -20°C Stability: ≥2 years Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures D-Glutamic acid is supplied as a crystalline solid. Aqueous solutions of D-glutamic acid can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of D-glutamic acid in PBS, pH 7.2, is approximately 1 mg/ml. We do not recommend storing the aqueous solution for more than one day. Description D-glutamic acid is an amino acid and a component of bacterial peptidoglycan.1 It is linked to UDP-N- acetylmuramoyl-L-alanine (UDP-MurNAc-L-Ala) by the muramyl ligase MurD to form UDP-MurNAc-L-Ala- D-glutamic acid, a building block in the biosynthesis of bacterial peptidoglycan. D-glutamic acid is also a component of poly-γ-glutamic acid, a polymer produced by Bacillus.2 References 1. Kouidmi, I., Levesque, R.C., and Paradis-Bleau, C. The biology of Mur ligases as an antibacterial target. Mol. Microbiol. 94(2), 242-253 (2014). 2. Ogasawara, Y., Shigematsu, M., Sato, S., et al. Involvement of peptide epimerization in poly-γ-glutamic acid biosynthesis. Org. Lett. 21(11), 3972-3975 (2019). WARNING CAYMAN CHEMICAL THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE. 1180 EAST ELLSWORTH RD SAFETY DATA ANN ARBOR, MI 48108 · USA This material should be considered hazardous until further information becomes available. -
Chess Autographs
Chess Autographs Welcome! My name is Gerhard Radosztics, I am living in Austria and I am a chess collector for many years. In the beginning I collected all stuff related to chess, especially stamps, first day covers, postmarks, postcards, phonecards, posters and autographs. In the last years I have specialised in Navigation Autograph Book Old chess postcards (click on card) A - M N - Z Single Chess autographs Links Contact On the next pages you can see a small part of my collection of autographs. The most of them are recognized, if you can recognize one of the unknown, please feel free to e-mail me. Note: The pages are very graphic intensive, so I ask for a little patience while loading. http://www.evrado.com/chess/autogramme/index.shtml[5/26/2010 6:13:18 PM] Autograph Book Autograph Book pages » back to previous page Page 1 - Introduction Page 18 - Marshall Page 2 - Aljechin Page 19 - Spielmann Page 3 - Lasker Page 19a - Capablanca Page 4 - Gruenfeld Page 20 - Canal Page 5 - Rubinstein Page 21 - Prokes Page 6 - Monticelli Page 22 - Euwe Page 7 - Mattisons Page 23 - Vidmar Page 8 - Asztalos Page 24 - Budapest 1948 Page 9 - Kmoch Page 25 - HUN - NED 1949 Page 10 - Gilg Page 25a - HUN - YUG 1949 Page 11 - Tartakover Page 26 - Budapest 1959 Page 12 - Nimzowitsch Page 26a - Budapest 1959 Page 13 - Colle Page 27 - Olympiad Leipzig Page 14 - Brinckmann Page 28 - Olympiad Leipzig Page 15 - Yates Page 29 - Budapest 1961 Page 16 - Kagan Page 30 - Spart.-Solingen 76 Page 17 - Maroczy http://www.evrado.com/chess/autogramme/autographindex.htm[5/26/2010 6:13:20 PM] Autogramme - Turniere - Namen Tournaments: » back to previous page 1 Sliac 1932 8 Dubrovnik 1950 15 Nizza 1974 2 Podebrady 1936 9 Belgrad 1954 16 Biel 1977 3 Semmering - Baden 1937 10 Zinnowitz 1967 17 Moskau 1994 4 Chotzen 1942 11 Polanica Zdroj 1967 18 Single autographs 5 Prag 1942 - Duras Memorial 12 Lugano 1968 19 World Champions Corr. -
Octot of $!)Ilos(Opl)P
Tilfe^^iJ BIOCHEMICAL STUDIES ON ANTIBIOTIC RESISTANCE AMONG STAPHYLOCOCCI FROM EYES AND OTHER CLINICAL SOURCES IN HEALTH AND DISEASE ABSTRACT THESIS SUBMITTED FOR THE AWARD OF THE DEGREE OF ©octot of $!)ilos(opl)p Piocfjemisitrp BY JAVID AHMAD DAR DEPARTMENT OF BIOCHEMISTRY & MICROBIOLOGY SECTION, INSTITUTE OF OPHTHALMOLOGY JAWAHARLAL NEHRU MEDICAL COLLEGE FACULTY OF MEDICINE ALIGARH MUSLIM UNIVERSITY ALIGARH-202002 (INDIA) 2003 •XBESlS Oor.-.V^'*^ Vsd •'« '4 .'-LGrcOd THESIS Biochemical Studies on Antibiotic Resistance among Staphylococci from Eyes and other Clinical Sources in Health and Disease Abstract The emergence of multidrug-resistant bacteria is a phenomenon of concem to the clinician and the pharmaceutical industry, as it is the major cause of failure in the treatment of infectious diseases. Staphylococci have a record of developing resistance quickly and successfully to antibiotics. This defensive response is a consequence of the acquisition and transfer of antibiotic resistance plasmids and the possession of intrinsic resistance mechanisms. The acquired defense systems by staphylococci may have originated from antibiotic-producing organisms, where they may have been developed and then passed on to other genera. There are reports of emergence and high occurrence of staphylococcus strains resistant to methicillin, especially MRSA, from various parts of the World. These organisms are mostly resistant to multiple antibiotics; therefore the treatment of infections due to these organisms and their eradication is very difficult. The present study was undertaken with the aim of determining epidemiology of clinical and carrier staphylococci and biochemical studies of their acquisition and dissemination of resistance. The prevalence of methicillin-resistant Staphylococci was determined in 750 subjects infected/colonized with staphylococci providing 850 isolates. -
GM Square Auction No. 20, 12-15 February 2009
GM Square Auction No. 20, 12-15 February 2009 http://www.gmsquare.com/chessauction/index.html Lot 1. BCM, volume I (1881, August to December) and volume II (1882, 392 pages, complete). Volume 1 is incomplete (starts from August; pages 257-400). Nicely bound as one volume together with indices for both years. Bound in half leather with marbled boards, marbled endpapers and edges, and gilt tickets to spine. Very good condition. Sold for €380.53 Lot 2. BCM, volume X (1890). 512 pages, complete with index. Nicely bound in half leather with marbled boards and endpapers, sprinkled edges and gilt tickets to spine. Fine condition. Sold for €394.08 Lot 3. BCM, volume XI (1891). 568 pages, complete with index. Nicely bound in half leather with marbled boards and endpapers, sprinkled edges and gilt ticket to spine. Light foxing to some pages, otherwise in fine condition. Sold for €410 Lot 4. BCM, volume XIII (1893). 558 pages, complete with index. Nicely bound in half leather with marbled boards and endpapers, and gilt ticket to spine. Very good condition. Sold for €316.99 Lot 5. BCM, volume No. 70 (1950). 412 pages, index. Bound in blue buckram, with gilt tickets to spine. Paper slightly browned, very good. Sold for €42.09 Lot 6. BCM, volumes No. 80-82 (1960, 1961 and 1962). 364, 364 and 372 pages respectively. All with index, all bound in blue buckram, with gilt tickets to spine. All are in very good condition. Sold for €66 Lot 7. Caissa magazine (Germany), volumes 2-7. (1948-1953).