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Eukaryotic Mrna Literature Flyer-20200806 Published in Nature Communications Armadillo Repeat Containing 12 Promotes Neuroblastoma Progression Through Interaction with Retinoblastoma Binding Protein 4 Background Armadillo (ARM) family proteins play an important role in tumor progression. This paper identified ARMC12 as an ARM member associated with neuroblastoma progression. 1 Materials 6 · Tumor cells (1×10 ) 2 RNA Isolation · Total RNA extracted using TRIzol® Research Pipeline 3 Library Construction · mRNA libraries for RNA-seq 4 Sequencing · Illumina platform 5 Bioinformatic Analysis · Differential expression · GSEA (Gene Set Enrichment Analysis) Research Results Armadillo repeat containing 12 (ARMC12 ), an ARM member associated with NB progression, promotes the growth and aggressiveness of NB cell lines. Mechanistically, ARMC12 physically interacts with retinoblastoma binding protein 4 (RBBP4) to facilitate the formation and activity of polycomb repressive complex 2 (PRC2), resulting in transcriptional repression of tumor suppressive genes. Blocking the interaction between ARMC12 and RBBP4 by cell-penetrating inhibitory peptide activates downstream gene expression and suppresses the tumorigenesis and aggressiveness of NB cells. Both ARMC12 and RBBP4 are upregulated in NB tissues, and are associated with unfavorable outcomes for patients. Analysis Showcase Novogene’s RNA-seq analysis helped to screen differentially expressed genes upon ARMC12 overexpression. Five genes, CADM1 , EGLN3 , HRK , HS6ST3 , and SMAD9 , were identified and further proved to be suppressed by ARMC12 , facilitating the growth and aggressiveness of NB cells. a k Moc ARMC12 100 Correlated genes Correlated genes HS6ST2 (GSE16476) (GSE62564) Down: Up: 327 3798 HS6ST3 CADM1 3224 2901 443 82 0 SMAD9 RNA-Seq ChIP-X EGLN3 105 MBNL3 (padj) 0 50 4493 0 10 HRK CHPT1 –1.04 852 14 0 MYO1B –0.69 –Log SETD7 –0.35 PPP1R3C 0.00 155 4 PLS1 0.35 0.69 1.3 TMEM45B 420 SLC7A11 1.04 0 4 8 –8 –4 12 16 –16 –12 Log2 (fold change) Volcano plots (left panel), Venn diagram (middle panel), and heatmap (right panel) show the alteration of gene expression in ARMC12 overexpressed or normal expressed cells. Red indicates high expression, and blue indicates low expression in the heatmap. Reference Subramanian, A. et al . Gene set enrichment analysis: A knowledge-basedapproach for interpreting genome-wide expres- sion profiles. Proc. Natl. Acad.Sci. USA 102, 15545‒15550 (2005). Powered Literature by Novogene Year Journal Title 2018 Cell Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2 -Aberrant Solid Tumors Armadillo repeat containing 12 promotes neuroblastoma progression through interaction 2018 Nat Commun with retinoblastoma binding protein 4 E6 protein expressed by high-risk HPV activates super-enhancers of the EGFR and c-MET 2018 Cancer Res oncogenes by destabilizing the histone demethylase KDM5C Pold3 is required for genomic stability and telomere integrity in embryonic stem cells Nucleic Acids Res 2018 and meiosis Single-Base Methylome Analysis Reveals Dynamic Epigenomic Differences Associated 2018 Plant Biotechnol J with Water Deficit in Apple A Critical Role of Presynaptic Cadherin/Catenin/ p140Cap Complexes in Stabilizing 2017 Neuron Spines and Functional Synapses in the Neocortex The genome of the medicinal plant Macleaya cordata provides new insights into 2017 Mol Plant benzylisoquinoline alkaloids metabolism SIRT6 Controls Hematopoietic Stem Cell Homeostasis through Epigenetic Regulation 2016 Cell Stem Cell of Wnt Signaling Novogene Corporation Inc. 8801 Folsom Blvd #290, Sacramento, CA 95826 916-252-0068-383 [email protected] en.novogene.com Follow us on LinkedIn.
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