bioRxiv preprint doi: https://doi.org/10.1101/027151; this version posted September 18, 2015. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Structure and evolutionary history of a large family of NLR 2 proteins in the zebrafish 3 4 5 Kerstin Howe1*, Philipp H. Schiffer2,3*, Julia Zielinski2, Thomas Wiehe2, Gavin K. Laird1, 6 John C. Marioni14, Onuralp Soylemez5,6, Fyodor Kondrashov5,6,7, Maria Leptin2,3# 7 8 1 Wellcome Trust Sanger Institute, Cambridge, United Kingdom 9 2 Institut für Genetik, Universität zu Köln, Köln, Deutschland 10 3 The European Molecular Biology Laboratory, Heidelberg, Germany 11 4 The European Molecular Biology Laboratory, The European Bioinformatics Institute 12 (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom 13 5 Bioinformatics and Genomics Programme, Centre for Genomic Regulation (CRG) 88 Dr. 14 Aiguader, 08003 Barcelona, Spain. 15 6 Universitat Pompeu Fabra (UPF), 08003 Barcelona, Spain. 16 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 23 Pg. Lluís Companys, 08010 17 Barcelona, Spain. 18 *Contributed equally 19 Keywords: NACHT, B30.2, SPRY, Pyrin, gene family expansion, gene conversion, innate 20 immune system, genome evolution 21 Running title: Evolution and structure of the NLR-B30.2 family 22 23
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[email protected], ORCiD: 0000-0001-7097-348X 32 33 #corresponding author: 34 Prof.