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Tests Des Composés De Nacre Sur L'activité Des Ostéoblastes Et Leur Identification

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Tests Des Composés De Nacre Sur L'activité Des Ostéoblastes Et Leur Identification Tests des composés de nacre sur l’activité des ostéoblastes et leur identification Ganggang Zhang To cite this version: Ganggang Zhang. Tests des composés de nacre sur l’activité des ostéoblastes et leur identification. Sciences du Vivant [q-bio]. Université de Lorraine, 2017. Français. NNT : 2017LORR0051. tel- 01757603 HAL Id: tel-01757603 https://hal.univ-lorraine.fr/tel-01757603 Submitted on 1 Jul 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 Code de la Propriété Intellectuelle. articles L 335.2- L 335.10 http://www.cfcopies.com/V2/leg/leg_droi.php http://www.culture.gouv.fr/culture/infos-pratiques/droits/protection.htm Ecole Doctorale BioSE (Biologie-Santé-Environnement) Thèse Présentée et soutenue publiquement pour l’obtention du titre de DOCTEUR DE L’UNIVERSITÉ DE LORRAINE Mention : «Science de la Vie et de la Santé» par : Ganggang ZHANG Tests des composés de nacre sur l'activité des ostéoblastes et leur identification Soutenue le 29/06/2017 Directeur de thèse : Pr. Pierre GILLET Co-encadrante de thèse : Dr. Marthe ROUSSEAU Membres du jury : Rapporteurs : M. Frédéric MARIN DR CNRS, UMR6282-CNRS/uB, Dijon M. Patrice LAQUERRIERE PU, UMR7178-CNRS/Unistra, Strasbourg Examinateurs : M. Pierre GILLET PU-PH, UMR7365-CNRS/UL, Nancy Mme. Marthe ROUSSEAU CR CNRS, UMR7365-CNRS/UL, Nancy Membre invité: M. Didier MAINARD PU-PH, CHU Nancy, Nancy UMR 7365-CNRS-Université de Lorraine. Laboratoire d’Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA) 9, Avenue de la Foret de Haye, Biopôle de l’Université de Lorraine, 54500 Vandoeuvre lès Nancy A mes parents qui m’aiment tellement beaucoup et me supportent tout le temps pendant mes études! Remerciements Je remercie Monsieur Frédéric MARIN et Monsieur Patrice LAQUERRIERE d’avoir accepté d’être les rapporteurs de cette thèse. Je remercie Monsieur Didier MAINARD pour avoir accepté d’être le membre invité de ma thèse. Je tiens à exprimer à Monsieur le professeur Pierre GILLET, directeur de ma thèse, ma profonde gratitude pour m’accueillir au sein de son équipe. Je tiens à exprimer à Madame Marthe ROUSSEAU, co-encadrante de ma thèse, ma gratitude distinguée et mon profond respect pour son accompagnement attentif sur mes travaux scientifiques, sa patience et tolérance incomparable, et tout son confort soigneux sur ma vie quotidienne. Elle m’a donné l’opportunité d’étendre mes recherches sur différents axes, de collaborer avec différentes équipes intra universitaires pour élargir mes connaissances scientifiques, et d’effectuer des communications nationales et internationales. Sa grande disponibilité a permis un suivi permanent du déroulement des travaux. Les discussions constantes entre nous m’ont beaucoup inspiré au niveau de la recherche scientifique et de la vie au cours de ces années de thèse. J’apprécie également son investissement dans la rédaction des articles et celle de ma thèse. Je lui suis profondément reconnaissant pour ses démarches et son dévouement aussi bien pour ma thèse que pour ma carrière. Je remercie mère patrie, China Scholarship Council et Université de Wuhan pour financer et supporter mes études en France. Je ne l’oublierai pas de toute ma vie. Ce travail a été réalisé dans le laboratoire d’Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS – Université de Lorraine au sein de la Faculté de Médecine de Nancy. Donc, je tiens à remercier Monsieur le Professeur Jean-Yves JOUZEAU, directeur de l'UMR CNRS 7365, pour m'avoir accepté dans l'unité de recherche et pour sa gentillesse. J’exprime aussi ma reconnaissance à Monsieur le Professeur Jean-François STOLTZ, qui m’a proposé de réaliser ma thèse dans cette unité, et ses encouragements constants sur mes travaux pendant toute ma thèse. Je remercie très sincèrement Madame Marie-Hélène PIET pour ses aides constantes sur les manipulations, notamment HPLC, et sur le français. Je remercie Madame Vanessa MOBY pour ses suggestions sur mes travaux et la langue française. Je souhaitais également remercier Alice BRION, notamment au début de ma thèse, elle m’a permis de me familiariser à la science en France et m’a fait apprendre presque toutes les techniques nécessaires pour effectuer mes manipulations expérimentales. Son attitude stricte sur les travaux m’a tellement touché. Je tiens à remercier Monsieur Vincent CARRÉ pour tant des suggestions sur mes travaux, pour la collaboration, et pour avoir accepté d’être membre de jury de mon comité de suivi de thèse. J’exprime ma gratitude à Monsieur Arnaud BIANCHI, Monsieur Cédric PARIS, Monsieur Frédéric AUBRIET, et Madame Virginie MARCHAND pour leurs aides professionnelles sur mes travaux. Les collaborations avec vous sont fructueuses, et m’ont permis de faire de belles rencontres. Je voudrais aussi remercier Monsieur Pascal REBOUL, Monsieur Alexandre KRIZNIK, Monsieur Jacques MAGDALOU, Madame Cécile GUILLAUME, Monsieur Frédéric CAILOTTO, Madame Isabelle BERTIN-JUNG pour leurs suggestions constructives sur mon projet de thèse ou sur mes manipulations. Je tiens également à remercier Monsieur le Professeur Didier MAINARD, directeur du COT, qui m’a fourni des prélèvements de l’os du genou et m’a aidé pour effectuer un stage d’observation de la chirurgie du rachis à Paris. Je remercie également Madame Monique GENTILS et Madame Brigitte GUERBER, Madame Karine LORCIN, Madame Valérie GOBERT, Madame Nadia BEZAI et Madame Ghislaine CHARPENTIER pour leur gentillesse à mon égard et leur soutien constant. Je remercie beaucoup à mes collègues français du laboratoire : Anne-Sophie, Gabriel, Benjamin, Mathilde, Julie, Anne, Romain, Léonore, Paul, Irfan, Caroline, Reine, Jacqueline, Hassan, Mathew, Mélissa, David, Meriem, Christophe et Jean-Marc pour m’avoir encouragé et supporté durant ces années. Vous allez me manquer. Un grand merci à la grande famille chinoise à Nancy: Qian chongsheng, Wei chaojie, Deng chaohua, Yu hao, Yang xu, Ye junsong, Liu xing, Hu yong, Xie zhe, Pang xiaomeng, Dan pan, Zhang lin, Xie yu, Luo yun, Wu xianglei, Duan jie, Li jie, Liang shiheng pour leur soutien et rires aux éclats durant ces années. Le meilleur pour la fin, je remercie toute ma famille, et plus particulièrement ma mère et mon père. A ma petite amie WEI Congcong, je t’aime, tu viens finalement après mon attente de 29 ans! Liste des publications et communications Revues à comité de lecture 3. Zhang G., Brion A., Willemin A-S., Piet M-H., Moby V., Bianchi A., Mainard D., Galois L., Gillet P., Rousseau M., Nacre, a natural, multi-use and timely biomaterial of bone graft substitute for current orthopaedic clinics, Journal of Biomedical Material Research Part A, 105(2):662-671, 2017. 2. Zhang G., Willemin A-S., Brion A., Piet M-H., Moby V., Bianchi A., Mainard D., Galois L., Gillet P., Rousseau M. A new method for the separation and purification of the nacre ethanol soluble matrix, Journal of structural biology, 196(2), 127-137, 2016. 1. Brion A.*, Zhang G.*, Dossot M., Moby V., Dumas D., Hupont S., Piet M.H., Bianchi A., Mainard D., Galois L., Gillet P., Rousseau M., Nacre extract restores the mineralization capacity of subchondral osteoarthritis osteoblasts, Journal of structural biology, 192(3), 500-9, 2015. (*Co-premier auteur) Communications orales dans des congrès internationaux (le nom de l’orateur est souligné) 1. Zhang G., Brion A., Piet MH., Moby V., Bianchi A., Mainard D., Galois L., Aubriet F., Carré V., Gillet P., Rousseau M., A new method for the purification and identification of the nacre ethanol soluble matrix, BIOMIN XIII, 13th International Symposium on Biomineralization, September 16-19, Granada, Spain, 2015. Communications affichées 4. Zhang G., Willemin A-S., Piet M-H., Moby V., Paris C., Bianchi A., Mainard D., Galois L., Aubriet F., Carré V., Gillet P., Rousseau M., An exploration into the mineral and organic composition of nacre ethanol soluble matrix towards the identification of osteogenic compounds, 18ème Journées Françaises de Biologie des Tissus Minéralisés, 1-3 Juin, Nancy, France, 2016. (Flash poster) 3. Zhang G., Willemin A-S., Brion A., Piet M-H., Moby V., Bianchi A., Mainard D., Galois L., Gillet P., Rousseau M., Cationic nacre ethanol soluble matrix has an osteoanabolic effect on human subchondral osteoarthritic osteoblasts and MC3T3-E1 cell line, 43th European Calcified Tissue Society Congress, May 14-17, Roma, Italy, 2016. ECTS/Eli Lilly 2016 Scholarship. 2. Zhang G., Brion A., Piet M-H, Moby V., Bianchi A., Dossot M., Mainard D., Galois L., Aubriet F., Carré V., Gillet P., Rousseau M., Separation and identification of the osteogenic compounds of nacre using ion-exchange resin associated with a mineralization cell model, XVII èmes Journées Françaises de Biologie des Tissus Minéralisés, Feburary 4-6, Clermont-Ferrant, France, 2015. 1. Brion A., Zhang G., Piet M.H., Moby V., Bianchi A., Dossot M., Mainard D., Galois L., Gillet P., Rousseau M., Pro-osteogenic properties of nacre extract on primary human osteoblasts and MC3T3-E1 cell line, 41st European Calcified Tissue Society Congress, May 17-20, Prague, Czech Republic, 2014 Sommaire Introduction !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! 01 1.
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