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UNICORN 5.0 User Reference Manual 03-0014-90/91 Edition AB 2004-02

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UNICORN 5.0 User Reference Manual 03-0014-90/91 Edition AB 2004-02 user manual chromatography software User Reference Manual um 03-0014-90 UNICORN 5.0 User Reference Manual 03-0014-90/91 Edition AB 2004-02 Office addresses: Amersham Biosciences AB SE-751 84 Uppsala Sweden Amersham Biosciences UK Limited Amersham Place Little Chalfont Buckinghamshire England HP7 9NA Amersham Biosciences Corp. 800 Centennial Avenue P.O. Box 1327 Piscataway NJ 08855 USA Amersham Biosciences Europe GmbH Munzinger Strasse 9 D-79111 Freiburg Germany Amersham Biosciences K.K. Sanken Building 3-25-1 Hyakunincho, Shinjuku-ku Tokyo 169-0073 Japan Amersham Biosciences China Limited 13/F., Tower I Ever Gain Plaza 88 Container Port Road Kwai Chung, New Territories Hong Kong www.amershambiosciences.com Trademarks: UNICORN, Drop Design, FPLCdirector, OligoPilot, BioProcess, Ettan, ÄKTA, ÄKTAxpress, ÄKTAbasic, ÄKTAexplorer, ÄKTAFPLC, ÄKTApilot and ÄKTApurifier are trademarks of Amersham Biosciences Limited. Amersham and Amersham Biosciences are trademarks of Amersham plc. Adobe, Acrobat and Distiller are trademarks of Adobe Systems Inc. Microsoft and Windows are trademarks of the Microsoft Corporation in the United States and/or other countries. Terms and Condition of Sale: Unless otherwise agreed in writing, all goods and services are sold subject to the terms and conditions of the company within the Amersham Biosciences group which supplies them. A copy of these terms and conditions is available on request. Any use of this software is subject to Amersham Biosciences Standard Software End-User License Agreement. © Copyright Amersham Biosciences AB 2004 - All rights reserved. Table of Contents Table of Contents 1. Introducing UNICORN..........................................................................................................9 1.1. About UNICORN.............................................................................................10 1.2. About this manual...........................................................................................14 1.3. About the UNICORN user documentation..........................................................17 2. UNICORN concepts............................................................................................................20 2.1. Concept definitions.........................................................................................21 2.2. The UNICORN user interface........................................................................................24 2.2.1. UNICORN Manager....................................................................................25 2.2.2. The Method Editor module.........................................................................28 2.2.3. The System Control module........................................................................32 2.2.4. The Evaluation module...............................................................................35 2.2.5. Search functions.......................................................................................37 2.2.6. Help functions and manuals.......................................................................39 2.2.7. Snapshots.................................................................................................41 2.3. Quick Start Guide............................................................................................44 3. General system operations.................................................................................................45 3.1. Log on routines and log off routines..................................................................46 3.2. How to create a new user.................................................................................50 3.3. How to assign user properties...........................................................................53 3.4. How to change your passwords and user attributes..............................................56 3.5. How to connect to the chromatography system..................................................58 3.6. How to back up and restore system data............................................................63 3.7. How to set up a printer....................................................................................66 4. Files and folders in UNICORN.............................................................................................67 4.1. How to create folders.......................................................................................68 4.2. How to open and preview files..........................................................................69 4.3. How to arrange and locate your files..................................................................72 4.4. How to copy, delete, rename and backup files and folders...................................75 5. How to create a method.....................................................................................................79 5.1. How to use the Method Wizard.........................................................................80 5.2. How to use the Method templates.....................................................................85 5.3. How to use Text instructions.............................................................................88 5.4. How to sign the method...................................................................................91 6. How to edit methods..........................................................................................................92 6.1. The Method Editor interface.........................................................................................93 • p i Table of Contents 6.1.1. Method Editor module................................................................................94 6.1.2. Text Instructions editor..............................................................................95 6.2. Method blocks............................................................................................................97 6.2.1. How to view method blocks........................................................................98 6.2.2. How to call method blocks........................................................................100 6.2.3. How to add method blocks.......................................................................101 6.2.4. How to delete method blocks ...................................................................104 6.2.5. How to rename method blocks..................................................................106 6.2.6. How to find, copy and move method blocks................................................107 6.2.7. How to import method blocks...................................................................109 6.3. Method instructions..................................................................................................111 6.3.1. How to read method instructions...............................................................112 6.3.2. How to add method instructions................................................................113 6.3.3. How to delete method instructions............................................................114 6.3.4. How to change or move method instructions...............................................115 6.4. How to use method variables..........................................................................118 6.5. Run Setup.................................................................................................................122 6.5.1. Overview of Run Setup.............................................................................123 6.5.2. The Variables tab.....................................................................................125 6.5.3. The Scouting tab.....................................................................................128 6.5.4. The Questions tab....................................................................................129 6.5.5. The Gradient tab.....................................................................................133 6.5.6. The Notes tab.........................................................................................135 6.5.7. The Evaluation Procedures tab..................................................................136 6.5.8. The Reference Curves tab.........................................................................140 6.5.9. The Columns tab.....................................................................................142 6.5.10. The BufferPrep tab................................................................................143 6.5.11. The Method Information tab...................................................................146 6.5.12. The Result Name tab.............................................................................147 6.5.13. The Frac-950 tab .................................................................................149 6.5.14. The Start Protocol tab............................................................................151 6.5.15. How to export the values in the Run Setup...............................................153 6.6. How to use selected method instructions....................................................................154 6.6.1. Base instruction......................................................................................155 6.6.2. Instructions at the same breakpoint...........................................................157 6.6.3. Block and method length.........................................................................158
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