Proposed Amendment to 21CFR124021
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Chelonian Perivitelline Membrane-Bound Sperm Detection: a New Breeding Management Tool
Zoo Biology 35: 95–103 (2016) RESEARCH ARTICLE Chelonian Perivitelline Membrane-Bound Sperm Detection: A New Breeding Management Tool Kaitlin Croyle,1,2 Paul Gibbons,3 Christine Light,3 Eric Goode,3 Barbara Durrant,1 and Thomas Jensen1* 1San Diego Zoo Institute for Conservation Research, Escondido, California 2Department of Biological Sciences, California State University San Marcos, San Marcos, California 3Turtle Conservancy, New York, New York Perivitelline membrane (PVM)-bound sperm detection has recently been incorporated into avian breeding programs to assess egg fertility, confirm successful copulation, and to evaluate male reproductive status and pair compatibility. Due to the similarities between avian and chelonian egg structure and development, and because fertility determination in chelonian eggs lacking embryonic growth is equally challenging, PVM-bound sperm detection may also be a promising tool for the reproductive management of turtles and tortoises. This study is the first to successfully demonstrate the use of PVM-bound sperm detection in chelonian eggs. Recovered membranes were stained with Hoechst 33342 and examined for sperm presence using fluorescence microscopy. Sperm were positively identified for up to 206 days post-oviposition, following storage, diapause, and/or incubation, in 52 opportunistically collected eggs representing 12 species. However, advanced microbial infection frequently hindered the ability to detect membrane-bound sperm. Fertile Centrochelys sulcata, Manouria emys,andStigmochelys pardalis eggs were used to evaluate the impact of incubation and storage on the ability to detect sperm. Storage at À20°C or in formalin were found to be the best methods for egg preservation prior to sperm detection. Additionally, sperm-derived mtDNA was isolated and PCR amplified from Astrochelys radiata, C. -
Box Turtles July 2017
The HERP Project, Herpetology Education in Rural Places and Spaces In Awe of Nature: Treasuring Terrestrial Turtles By Ann Berry Somers, Catherine Matthews, and Lacey Huffling The Herp Project is supported by the National Science Foundation, Grant No. DRL-1114558. Any opinions, findings, and conclusions or recommendations expressed in this manuscript are those of the authors and do not necessarily reflect the views of the National Science Foundation. Treasuring Terrestrial Turtles Before starting a project like the one described in this curriculum, contact your state wildlife resources commission or state division of fish and game to see what permits you need to work with box turtles. I. Project Description This curriculum was developed by The HERP (Herpetology Education in Rural Places and Spaces) Project to introduce participants to the wonders of nature and science through the study of box turtles (Terrapene spp.). The curriculum was developed over several years of working with high school students in our Herpetological Research Experience (HRE) residential program. Feel free to modify this curriculum as needed. In our program, participants are introduced to turtle biology as well as to The Box Turtle Connection (BTC), our long-term mark/recapture box turtle study in North Carolina. The BTC is designed to follow temporal trends in population size and structure (sex, age class) as well as the health and condition of individual box turtles from numerous sites across North Carolina. The data collected are important to help scientists determine if box turtles need special conservation measures to maintain their populations and thrive in their natural habitat. Our box turtle studies are enhanced by use of Boykin Spaniel dogs to locate and retrieve box turtles and use of radio tracking to determine activity ranges for male and female box turtles. -
The Case of Deirocheline Turtles
bioRxiv preprint doi: https://doi.org/10.1101/556670; this version posted February 21, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Body coloration and mechanisms of colour production in Archelosauria: 2 The case of deirocheline turtles 3 Jindřich Brejcha1,2*†, José Vicente Bataller3, Zuzana Bosáková4, Jan Geryk5, 4 Martina Havlíková4, Karel Kleisner1, Petr Maršík6, Enrique Font7 5 1 Department of Philosophy and History of Science, Faculty of Science, Charles University, Viničná 7, Prague 6 2, 128 00, Czech Republic 7 2 Department of Zoology, Natural History Museum, National Museum, Václavské nám. 68, Prague 1, 110 00, 8 Czech Republic 9 3 Centro de Conservación de Especies Dulceacuícolas de la Comunidad Valenciana. VAERSA-Generalitat 10 Valenciana, El Palmar, València, 46012, Spain. 11 4 Department of Analytical Chemistry, Faculty of Science, Charles University, Hlavova 8, Prague 2, 128 43, 12 Czech Republic 13 5 Department of Biology and Medical Genetics, 2nd Faculty of Medicine, Charles University and University 14 Hospital Motol, V Úvalu 84, 150 06 Prague, Czech Republic 15 6 Department of Food Science, Faculty of Agrobiology, Food, and Natural Resources, Czech University of Life 16 Sciences, Kamýcká 129, Prague 6, 165 00, Czech Republic 17 7 Ethology Lab, Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, C/ 18 Catedrátic José Beltrán Martinez 2, Paterna, València, 46980, Spain 19 Keywords: Chelonia, Trachemys scripta, Pseudemys concinna, nanostructure, pigments, chromatophores 20 21 Abstract 22 Animal body coloration is a complex trait resulting from the interplay of multiple colour-producing mechanisms. -
AN INTRODUCTION to Texas Turtles
TEXAS PARKS AND WILDLIFE AN INTRODUCTION TO Texas Turtles Mark Klym An Introduction to Texas Turtles Turtle, tortoise or terrapin? Many people get confused by these terms, often using them interchangeably. Texas has a single species of tortoise, the Texas tortoise (Gopherus berlanderi) and a single species of terrapin, the diamondback terrapin (Malaclemys terrapin). All of the remaining 28 species of the order Testudines found in Texas are called “turtles,” although some like the box turtles (Terrapene spp.) are highly terrestrial others are found only in marine (saltwater) settings. In some countries such as Great Britain or Australia, these terms are very specific and relate to the habit or habitat of the animal; in North America they are denoted using these definitions. Turtle: an aquatic or semi-aquatic animal with webbed feet. Tortoise: a terrestrial animal with clubbed feet, domed shell and generally inhabiting warmer regions. Whatever we call them, these animals are a unique tie to a period of earth’s history all but lost in the living world. Turtles are some of the oldest reptilian species on the earth, virtually unchanged in 200 million years or more! These slow-moving, tooth less, egg-laying creatures date back to the dinosaurs and still retain traits they used An Introduction to Texas Turtles | 1 to survive then. Although many turtles spend most of their lives in water, they are air-breathing animals and must come to the surface to breathe. If they spend all this time in water, why do we see them on logs, rocks and the shoreline so often? Unlike birds and mammals, turtles are ectothermic, or cold- blooded, meaning they rely on the temperature around them to regulate their body temperature. -
Buhlmann Etal 2009.Pdf
Chelonian Conservation and Biology, 2009, 8(2): 116–149 g 2009 Chelonian Research Foundation A Global Analysis of Tortoise and Freshwater Turtle Distributions with Identification of Priority Conservation Areas 1 2 3 KURT A. BUHLMANN ,THOMAS S.B. AKRE ,JOHN B. IVERSON , 1,4 5 6 DENO KARAPATAKIS ,RUSSELL A. MITTERMEIER ,ARTHUR GEORGES , 7 5 1 ANDERS G.J. RHODIN ,PETER PAUL VAN DIJK , AND J. WHITFIELD GIBBONS 1University of Georgia, Savannah River Ecology Laboratory, Drawer E, Aiken, South Carolina 29802 USA [[email protected]; [email protected]]; 2Department of Biological and Environmental Sciences, Longwood University, 201 High Street, Farmville, Virginia 23909 USA [[email protected]]; 3Department of Biology, Earlham College, Richmond, Indiana 47374 USA [[email protected]]; 4Savannah River National Laboratory, Savannah River Site, Building 773-42A, Aiken, South Carolina 29802 USA [[email protected]]; 5Conservation International, 2011 Crystal Drive, Suite 500, Arlington, Virginia 22202 USA [[email protected]; [email protected]]; 6Institute for Applied Ecology Research Group, University of Canberra, Australian Capitol Territory 2601, Canberra, Australia [[email protected]]; 7Chelonian Research Foundation, 168 Goodrich Street, Lunenburg, Massachusetts 01462 USA [[email protected]] ABSTRACT. – There are currently ca. 317 recognized species of turtles and tortoises in the world. Of those that have been assessed on the IUCN Red List, 63% are considered threatened, and 10% are critically endangered, with ca. 42% of all known turtle species threatened. Without directed strategic conservation planning, a significant portion of turtle diversity could be lost over the next century. Toward that conservation effort, we compiled museum and literature occurrence records for all of the world’s tortoises and freshwater turtle species to determine their distributions and identify priority regions for conservation. -
Dispersal in the Coahuilan Box Turtle
1 Running title: Dispersal in the Coahuilan box turtle In press, Molecular Ecology 2 3 4 CONTRASTING DEMOGRAPHIC AND GENETIC ESTIMATES OF DISPERSAL IN THE 5 6 ENDANGERED COAHUILAN BOX TURTLE: A CONTEMPORARY APPROACH TO 7 8 CONSERVATION 9 10 Jennifer G. Howeth1,4, Suzanne E. McGaugh2, Dean A. Hendrickson1,3 11 12 MEC-08-0399 Final 13 1 Section of Integrative Biology 14 University of Texas at Austin 15 1 University Station C0930 16 Austin, Texas 78712 17 Phone: 512 475 8669 18 Fax: 512 471 3878 19 20 2 Department of Ecology, Evolution, and Organismal Biology 21 Iowa State University 22 251 Bessey Hall 23 Ames, Iowa 50011 24 25 3 Texas Natural Science Center, Texas Natural History Collection 26 University of Texas at Austin 27 PRC 176 / R4000 28 10100 Burnet Road 29 Austin, Texas 78758 30 31 4 Corresponding author: [email protected] 32 Abstract: 249 / 250 words 33 Main text: 7,641 / 8,000 words 34 Figures: 5; Supplementary Figures: 2 35 Tables: 3; Supplementary Tables: 2 36 37 38 Keywords: connectivity, isolation by distance, metapopulation, microsatellite, mark-recapture, 39 habitat fragmentation 40 Abstract 41 42 The evolutionary viability of an endangered species depends upon gene flow among subpopulations 43 and the degree of habitat patch connectivity. Contrasting population connectivity over ecological and 44 evolutionary timescales may provide novel insight into what maintains genetic diversity within 45 threatened species. We employed this integrative approach to evaluating dispersal in the critically 46 endangered Coahuilan box turtle (Terrapene coahuila) that inhabits isolated wetlands in the desert- 47 spring ecosystem of Cuatro Ciénegas, Mexico. -
Manual for the Differentiation of Captive-Produced and Wild-Caught Turtles and Tortoises (Testudines)
Image: Peter Paul van Dijk Image:Henrik Bringsøe Image: Henrik Bringsøe Image: Andrei Daniel Mihalca Image: Beate Pfau MANUAL F O R T H E DIFFERENTIATION OF CAPTIVE-PRODUCED AND WILD-CAUGHT TURTLES AND TORTOISES (TESTUDINES) PREPARED BY SPECIES360 UNDER CONTRACT FOR THE CITES SECRETARIAT Manual for the differentiation of captive-produced and wild-caught turtles and tortoises (Testudines) This document was prepared by Species360 under contract for the CITES Secretariat. Principal Investigators: Prof. Dalia A. Conde, Ph.D. and Johanna Staerk, Ph.D., Species360 Conservation Science Alliance, https://www.species360.orG Authors: Johanna Staerk1,2, A. Rita da Silva1,2, Lionel Jouvet 1,2, Peter Paul van Dijk3,4,5, Beate Pfau5, Ioanna Alexiadou1,2 and Dalia A. Conde 1,2 Affiliations: 1 Species360 Conservation Science Alliance, www.species360.orG,2 Center on Population Dynamics (CPop), Department of Biology, University of Southern Denmark, Denmark, 3 The Turtle Conservancy, www.turtleconservancy.orG , 4 Global Wildlife Conservation, globalwildlife.orG , 5 IUCN SSC Tortoise & Freshwater Turtle Specialist Group, www.iucn-tftsG.org. 6 Deutsche Gesellschaft für HerpetoloGie und Terrarienkunde (DGHT) Images (title page): First row, left: Mixed species shipment (imaGe taken by Peter Paul van Dijk) First row, riGht: Wild Testudo marginata from Greece with damaGe of the plastron (imaGe taken by Henrik BrinGsøe) Second row, left: Wild Testudo marginata from Greece with minor damaGe of the carapace (imaGe taken by Henrik BrinGsøe) Second row, middle: Ticks on tortoise shell (Amblyomma sp. in Geochelone pardalis) (imaGe taken by Andrei Daniel Mihalca) Second row, riGht: Testudo graeca with doG bite marks (imaGe taken by Beate Pfau) Acknowledgements: The development of this manual would not have been possible without the help, support and guidance of many people. -
The Conservation Biology of Tortoises
The Conservation Biology of Tortoises Edited by Ian R. Swingland and Michael W. Klemens IUCN/SSC Tortoise and Freshwater Turtle Specialist Group and The Durrell Institute of Conservation and Ecology Occasional Papers of the IUCN Species Survival Commission (SSC) No. 5 IUCN—The World Conservation Union IUCN Species Survival Commission Role of the SSC 3. To cooperate with the World Conservation Monitoring Centre (WCMC) The Species Survival Commission (SSC) is IUCN's primary source of the in developing and evaluating a data base on the status of and trade in wild scientific and technical information required for the maintenance of biological flora and fauna, and to provide policy guidance to WCMC. diversity through the conservation of endangered and vulnerable species of 4. To provide advice, information, and expertise to the Secretariat of the fauna and flora, whilst recommending and promoting measures for their con- Convention on International Trade in Endangered Species of Wild Fauna servation, and for the management of other species of conservation concern. and Flora (CITES) and other international agreements affecting conser- Its objective is to mobilize action to prevent the extinction of species, sub- vation of species or biological diversity. species, and discrete populations of fauna and flora, thereby not only maintain- 5. To carry out specific tasks on behalf of the Union, including: ing biological diversity but improving the status of endangered and vulnerable species. • coordination of a programme of activities for the conservation of biological diversity within the framework of the IUCN Conserva- tion Programme. Objectives of the SSC • promotion of the maintenance of biological diversity by monitor- 1. -
Species Results from Database Search
Species Results From Database Search Category Reptiles Common Name Alabama Map Turtle Scientific Name Graptemys pulchra LCC Global Trust N No. of States 1 Habitat_Feature Category Reptiles Common Name Black Kingsnake Scientific Name Lampropeltis getula nigra LCC Global Trust N No. of States 2 Habitat_Feature Category Reptiles Common Name Black Racer Scientific Name Coluber constrictor constrictor LCC Global Trust N No. of States 1 Habitat_Feature Category Reptiles Common Name Black Rat Snake Scientific Name Elaphe obsoleta LCC Global Trust N No. of States 2 Habitat_Feature Category Reptiles Common Name Bog turtle Scientific Name Clemmys (Glyptemys) muhlen LCC Global Trust Y No. of States 4 Habitat_Feature Monday, January 28, 2013 Page 1 of 14 Category Reptiles Common Name Broadhead Skink Scientific Name Eumeces laticeps LCC Global Trust N No. of States 5 Habitat_Feature Category Reptiles Common Name Coal Skink Scientific Name Eumeces anthracinus LCC Global Trust Y No. of States 8 Habitat_Feature Category Reptiles Common Name Common Five-lined Skink Scientific Name Eumeces fasciatus LCC Global Trust N No. of States 2 Habitat_Feature Category Reptiles Common Name Common Map Turtle Scientific Name Graptemys geographica LCC Global Trust N No. of States 6 Habitat_Feature Category Reptiles Common Name Common Musk Turtle Scientific Name Sternotherus odoratus LCC Global Trust N No. of States 2 Habitat_Feature Monday, January 28, 2013 Page 2 of 14 Category Reptiles Common Name Common Ribbonsnake Scientific Name Thamnophis sauritus sauritus LCC Global Trust N No. of States 6 Habitat_Feature Category Reptiles Common Name Common Snapping Turtle Scientific Name Chelydra serpentina LCC Global Trust N No. of States 2 Habitat_Feature Category Reptiles Common Name Corn snake Scientific Name Elaphe guttata guttata LCC Global Trust N No. -
Keanekaragaman Reptil Impor Di Yogyakarta
Biota Vol. 1 (3): 117−125, Oktober 2016 ISSN 2527-323X Keanekaragaman Reptil Impor di Yogyakarta Diversity of Imported Reptiles in Yogyakarta Dicky Indar Putranto*, Pramana Yuda, Felicia Zahida Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jln. Babarsari 44 Yogyakarta 55281 E-mail: [email protected] *Penulis untuk korespondensi Abstract Imported reptiles are in great demand because they have a wide variety of colors. This research is about exotic reptile species in Yogyakarta which are preserved or detached or released in the wild; and their potential impacts for local reptile species in Yogyakarta. This research was conducted in urban sites of Yogyakarta by conducting survey on animal markets, pet shops and reptile owners. This research was conducted from August 1, 2013 to November 30, 2013. Based on the result of the survey of imported reptile data collection in Yogyakarta, there was found 80 species, consisting of one species of pygmy crocodiles (Paleosuchus palpebrosus), 14 species of lizards (Sauria), 21 species of serpentes (Serpentes), and 44 species of turtles (Testudines). Imported reptiles released in nature were found in several numbers of species, namely two Red Eared Sliders (Trachemys scripta elegans), three Chinese Soft-shelled Turtles (Pelodiscus sinensis) and one Corn snake (Pantherophis guttatus). Red Eared Sliders which were released in the wild in such number cannot have a negative impact on local reptiles, but if in large quantities, this species is likely to be a potential competitor for bulus jawa (Amyda cartilaginea) in foraging for food. Chinese Soft-shelled Turtle were released in nature. In such number, it is likely to be a potential competitor for Amyda cartilaginea in foraging for food. -
Morphological Variation in the Brazilian Radiated Swamp Turtle Acanthochelys Radiolata (Mikan, 1820) (Testudines: Chelidae)
Zootaxa 4105 (1): 045–064 ISSN 1175-5326 (print edition) http://www.mapress.com/j/zt/ Article ZOOTAXA Copyright © 2016 Magnolia Press ISSN 1175-5334 (online edition) http://doi.org/10.11646/zootaxa.4105.1.2 http://zoobank.org/urn:lsid:zoobank.org:pub:DD15BE49-9A20-454E-A1FF-9E650C2A5A84 Morphological variation in the Brazilian Radiated Swamp Turtle Acanthochelys radiolata (Mikan, 1820) (Testudines: Chelidae) RAFAELLA C. GARBIN1,4,5, DEBORAH T. KARLGUTH2, DANIEL S. FERNANDES2,4, ROBERTA R. PINTO3,4 1Département de Géosciences, Université de Fribourg, Fribourg, 1700, Switzerland 2Departamento de Zoologia, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro, RJ, 21941-902, Brazil 3Universidade Católica de Pernambuco, Rua do Príncipe 526, Boa Vista, Recife, PE, 50050-900, Brazil 4Departamento de Vertebrados, Museu Nacional, Universidade Federal do Rio de Janeiro, Quinta da Boa Vista, Rio de Janeiro, RJ, 20940-40, Brazil 5Corresponding author. E-mail: [email protected] Abstract The freshwater turtle Acanthochelys radiolata (Mikan, 1820) is endemic to the Atlantic Forest domain in Brazil and few studies have been done on the morphology, geographic variation and taxonomy of this species. In this paper we record the morphological variation, as well as sexual dimorphism and ontogenetic changes in A. radiolata throughout its distribution range. We analyzed 118 morphological characters from 41 specimens, both quantitative and qualitative, and performed statistical analyses to evaluate size and shape variation within our sample. Morphological analysis revealed that A. radi- olata is a polymorphic species, especially regarding color and shape. Two color patterns were recognized for the carapace and three for the plastron. -
ECUADOR – Galapagos Giant Tortoises Stolen From
CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIES OF WILD FAUNA AND FLORA NOTIFICATION TO THE PARTIES No. 2018/076 Geneva, 30 October 2018 CONCERNING: ECUADOR Galapagos giant tortoises stolen from breeding center 1. This Notification is being published at the request of Ecuador. 2. The CITES Management Authority of Ecuador informed the Secretariat that on 27 September 2018, the Galapagos National Park Directorate filed a criminal complaint in Ecuador following the theft of 123 live Galapagos giant tortoises (Chelonoidis niger) from the Galapagos National Park breeding center on Isabela Island. 3. The Galapagos giant tortoise (Chelonoidis niger1) is included in CITES Appendix I. 4. The stolen tortoises range from one to six years in age. One-year-old Galapagos giant tortoises may be around six centimetres in carapace length and weigh an estimated 200 grams. A six-year-old Galapagos giant tortoise could range from 12 to 30 centimetres in carapace length, and weigh around two kilograms. 5. The likely market for the stolen specimens is outside of Ecuador, and the CITES Management Authority of Ecuador therefore requests that the present Notification be distributed as widely as possible among police, customs and wildlife enforcement authorities. 6. Parties are requested to inform the CITES Management Authority of Ecuador should any permits or certificates regarding trade in these specimens be received. The Management Authority of Ecuador also requests that CITES Management Authorities do not approve any export, import or re-export permit applications related to this species before consulting with the CITES Management Authority of Ecuador. 7. Parties that seize illegally traded specimens of Chelonoidis niger are also requested to communicate information about these seizures to the Management Authority of Ecuador.