Specifications and Standards for Foods, Food Additives, Etc. Under the Food Sanitation Act (Abstract) 2010

Total Page:16

File Type:pdf, Size:1020Kb

Specifications and Standards for Foods, Food Additives, Etc. Under the Food Sanitation Act (Abstract) 2010 Specifications and Standards for Foods, Food Additives, etc. Under the Food Sanitation Act (Abstract) 2010 April 2011 Copyright © 2011 JETRO. All rights reserved. CONTENTS PREFACE .................................................................................................................................................... 1 I. FOOD ........................................................................................................................................................ 5 1. Specifications and Standards for Food in General and for Individual Food Categories .................. 5 2. Maximum Residue Limits for Agricultural Chemicals (Pesticides), Feed Additives and Veterinary Drugs in Food ..................................................................................................................... 6 3. Provisional Regulatory Limitations of Contaminants in Food .......................................................... 6 4. Genetically modified foods ................................................................................................................... 6 5. Foods for Specified Health Uses and Foods with Nutrient Function Claims ................................... 7 6. Food Labeling ....................................................................................................................................... 8 6-1. Establishment of Consumer Affairs Agency .............................................................................. 10 6-2. Labeling of Food Consumption Date Limits .............................................................................. 10 6-3. Labeling of Genetically Modified Foods / Foods that can cause food allergy ........................... 11 6-4. Food for Specified Health Uses (FOSHU) / Food with Nutrient Function Claims (FNFC) ..... 13 7. Discontinuation of “Pilot Sample System" for Import Clearance of Food ....................................... 14 II. MILK AND MILK PRODUCTS ......................................................................................................... 15 III. FOOD ADDITIVES .......................................................................................................................... 17 1. Designated Food Additives ................................................................................................................ 17 2. Standards for Manufacturing Food Additives .................................................................................. 18 3. Standards for Use of Food Additives in General .............................................................................. 19 4. Food Additives with Standards for the Use, and Food Additives with No Standard for the Use .. 19 5. Existing Food Additives ..................................................................................................................... 20 6. List of source substances of natural flavoring agents ...................................................................... 20 7. Substances which are generally provided as food and which are used as additives (“Ordinary foods used as additives”)............................................................................................................ 20 8. Methods for labeling additives in food .............................................................................................. 21 IV. Apparatus and Containers / Packages ............................................................................................. 23 V. TOYS ................................................................................................................................................... 24 VI. CLEANING AGENTS (DETERGENTS).......................................................................................... 26 Copyright © 2011 JETRO. All rights reserved. (Tables) I. Food ...................................................................................................................................................... 27 Table F01 Specifications and Standards for Food in General ........................................................... 27 Table F02 Specifications and Standards for Individual Food Categories ........................................ 29 Table F03 Maximum Residue Limits for Agricultural Chemicals (Pesticides), Feed Additives and Veterinary Drugs in Food ................................................................................................ 38 Table F04 Provisional Regulatory Limitations of Contaminants in Food ........................................ 39 Table F05 Genetically Modified Foods ............................................................................................... 40 II. Milk and Milk Products .................................................................................................................... 42 Table M01 Raw Milk ........................................................................................................................... 42 Table M02 Liquid Milk for drinking and Milk Beverage .................................................................. 43 Table M03 Milk Products.................................................................................................................... 45 Table M04 Yogurts and Fermented Milk Drinks ............................................................................... 49 Table M05 Products Storable at Room Temperature ........................................................................ 49 III. Food additives .................................................................................................................................. 50 Table FA01 Designated Food Additives ............................................................................................. 50 Table FA02 Food Additives with Standards of Use ......................................................................... 111 Table FA03 Food Additives with No Standards of Use ................................................................... 148 Table FA04 Existing Food Additives ................................................................................................ 153 Table FA05 List of substances of Natural Flavoring Agents........................................................... 163 Table FA06 Substances which are generally provided as Food and used as Food Additives ........ 167 IV. Apparatus and Containers/ Packages ............................................................................................ 169 Table AP01 Standards on Material in General................................................................................ 169 Table AP02 Specifications and Standards by Material ................................................................... 170 Table AP03 Specifications and Standards by Use ........................................................................... 176 Table AP04 Standards of Manufacturing......................................................................................... 179 Table AP05 Specifications and Standards for Milk and Milk products .......................................... 180 Copyright © 2011 JETRO. All rights reserved. PREFACE This publication has been prepared to present an outline of specifications and standards for foods, milk and milk products, food additives, apparatus and container / packages, which are regulated under the Food Sanitation Act and relevant legislations. However, this publication has been produced with a focus mainly on user-friendliness: reference should be made to the original legislation, in order to confirm compliance therewith. Herein presents a summary of the current situation in Japan, as of December 31, 2010, of specifications and standards set out in the “Food Sanitation Act”, “Ordinance for Enforcement of the Food Sanitation Act”, “Ministerial Ordinance Concerning Compositional Standards, etc. for Milk and Milk Products”, relevant legislations (e.g., the Health Promotion Act), notices and announcements issued by the Ministry of Health, Labour and Welfare (MHLW), as well as legislations issued by the Consumer Affairs Agency (CAA) regarding food labeling. The Food Sanitation Act also applies mutatis mutandis to toys infants may put into their mouths, as well as cleaning agents (detergents) intended for use in washing vegetables, fruits, or tableware. With regards to food labeling, the Consumer Affairs Agency was established on September 1, 2009, to hold overall jurisdiction over food labeling, which was formally under the jurisdiction of the MHLW, as far as regulations for the labeling are concerned. Likewise, with regards to the JAS Law which used to be under the jurisdiction of the Ministry of Agriculture, Forestry and Fisheries, the overall jurisdiction was transferred to the Consumer Affairs Agency as far as the regulations for the labeling are concerned. The Consumer Affairs Agency was to hold overall jurisdiction over food labeling, including measures to be undertaken to ensure compliance with Codex standards. In the context of this publication’s theme, i.e., “specifications and standards for foods, food additives, etc.,” the Ministry of Health and Welfare Notification No. 370, 1959 (latest revision: MHLW Notification No.336, 2010) under the title of “Specifications and Standards for Food and Food Additives etc.” is an important piece of legislation. Therein are set out all the specifications and standards for the following: foods; food additives; apparatus and containers/ packages; toys; and cleaning
Recommended publications
  • Pulegone [89-82-7]
    Integrated Laboratory Systems Pulegone [89-82-7] and One Of Its Metabolites Menthofuran [494-90-6] Review of Toxicological Literature Prepared for Errol Zeiger, Ph.D. National Institute of Environmental Health Sciences P.O. Box 12233 Research Triangle Park, North Carolina 27709 Contract No. N01-ES-65402 Submitted by Raymond Tice, Ph.D. Integrated Laboratory Systems P.O. Box 13501 Research Triangle Park, North Carolina 27709 January 1998 EXECUTIVE SUMMARY The nomination of pulegone and menthofuran for testing is based on the potential for human exposure and the absence of carcinogenicity data. Pulegone and menthofuran are available from suppliers of laboratory test chemicals while pennyroyal oil with pulegone as a major constituent is commonly available in health food stores. Pulegone can be synthetically produced but no data were found concerning the synthetic production process; pulegone can also be produced by shoot cultures of Mentha piperita grown in fermenters. No data were found on production or import volumes. Pulegone is a major constituent of several essential oils (e.g., peppermint, pennyroyal) used for flavoring foods and drinks. Pennyroyal oil, which has been reported to contain 75-85% pulegone, has also been used as a fragrance agent and as an herbal medicine to induce menstruation and abortion. A number of plant species contain pulegone and menthofuran, including numerous species of mints such as peppermint and spearmint, the pennyroyals, and mountain mints. Menthofuran is present in peppermint and in M. aquatica and its hybrids. Exposure to pulegone and menthofuran is primarily through ingestion of food products (e.g., frozen dairy dessert, candy, baked goods, gelatins, and puddings) and of alcoholic and nonalcoholic beverages flavored with spearmint oil, peppermint oil, or synthetic pulegone.
    [Show full text]
  • Antiradical and Antibacterial Activity of Essential Oils from the Lamiaceae Family Plants in Connection with Their Composition and Optical Activity of Components
    International Journal of Secondary Metabolite 2018, Vol. 5, No. 2, 109–122 DOI: 10.21448/ijsm.408165 Published at http://www.ijate.net http://dergipark.gov.tr/ijsm Research Article Antiradical and Antibacterial Activity of Essential Oils from the Lamiaceae Family Plants in Connection with their Composition and Optical Activity of Components Hanna G. Shutava 1, Tatsiana G. Shutava 2, Natalya A. Kavalenka3, Halina N. Supichenka3 1 Central Botanical Garden, National Academy of Sciences of Belarus, Minsk, Belarus 2 Institute of Chemistry of New Materials, National Academy of Sciences of Belarus, Minsk, Belarus 3 Belarusian State Technological University, Minsk, Belarus Abstract: Antiradical activity of essential oils of korean mint (Agastache rugosa ARTICLE HISTORY (Fisch. et Mey)), blue giant hyssop (Agastache foeniculum (Pursh) Kuntze), hyssop Received: 12 January 2018 (Hyssopus officinalis L.), lavander (Lavandula angustifolia L.), peppermint (Mentha piperita L.), lemon mint (Mentha piperita var. citrata (Ehrh.) Briq), Revised: 11 March 2018 monarda (Monarda fistulosa L.), oregano (Origanum vulgare L.), common sage Accepted: 20 March 2018 (Salvia officinalis L.), clary (Salvia sclarea L.), and winter savory (Satureja montana L.) cultivated in the Central Botanical Garden of NAS of Belarus was KEYWORDS investigated in the reaction with the cation-radicals of 2,2-azino-bis(3- ethylbenzothiazoline-6-sulphonic acid) (ABTS+). The most pronounced Oil composition; antiradical activity was observed for the essential oils with a high content of Antiradical activity, phenolic compounds: winter savory and monarda. Antiradical properties of the Antibacterial activity, essential oils and individual phenolic and terpene compounds (eugenol, carvacrol, Enantiomer, thymol, citral, (+)-pulegone) in the reaction with ABTS+ significantly differ in Lamiaceae aqueous solutions and ethanol-water mixtures.
    [Show full text]
  • MCKENNA-THESIS-2016.Pdf
    IDENTIFICATION AND CHARACTERIZATION OF MINT LACTONES: TRACE-LEVEL ODOR-IMPORTANT AROMA COMPOUNDS IN PEPPERMINT OIL BY SAMANTHA GRACE MCKENNA THESIS Submitted in partial fulfillment of the requirements for the degree of Master of Science in Food Science and Human Nutrition with a concentration in Food Science in the Graduate College of the University of Illinois at Urbana-Champaign, 2016 Urbana, Illinois Master’s Committee: Professor Keith R. Cadwallader, Chair and Adviser Professor Nicki J. Engeseth Professor Shelly J. Smith ABSTRACT Mentha piperita L., commonly known as peppermint, is an essential oil bearing plant that was introduced to the United States in the early-19th century. By 1900 peppermint cultivation and oil production had reached the Pacific Northwest where the majority of peppermint oil in the United States is produced today. In 2015 the United States produced 5.9 million pounds of peppermint oil which is used in a wide variety of food and flavor applications including chewing gum, oral hygiene products, pharmaceuticals, confectionary, and liquor among others. Trace odorants are crucial to the flavor profile of well-balanced peppermint oil. Certain lactones have been identified in peppermint oils in trace levels that contribute important sweet, coconut, coumarin-like aroma characteristics to the oils. Among these lactones, collectively known as “mint lactones”, (−)-mintlactone and (+)-isomintlactone are the most well characterized, although numerous others remain unidentified. Four peppermint oils were analyzed by gas chromatography-olfactometry (GCO) and gas chromatography-mass spectrometry (GC-MS) to identify the odor-active lactones. Since lactones are present at trace levels, a silica gel fractionation method was developed to isolate and concentrate the lactones from peppermint oil prior to GC analysis.
    [Show full text]
  • (R)-(+)-Pulegone, a Chemical Constituent of Essential Oils Damião P
    Pharmacological Activity of (R)-(+)-Pulegone, a Chemical Constituent of Essential Oils Damião P. de Sousaa, Franklin F. F. Nóbregab, Maria R. V. de Limab, and Reinaldo N. de Almeidab,* a Department of Physiology, Federal University of Sergipe, CEP 49100–000, Aracaju, Sergipe, Brazil b Laboratório de Tecnologia Farmacêutica, Federal University of Paraíba, Caixa Postal 5009, CEP 58051–970, João Pessoa, Paraíba, Brazil. E-mail: [email protected] * Author for correspondence and reprint requests Z. Naturforsch. 66 c, 353 – 359 (2011); received April 24, 2010/March 10, 2011 (R)-(+)-Pulegone is a monoterpene found in essential oils from plants of the Labiatae family. This compound is a major constituent of Agastache formosanum oil. In this study, the effect of (R)-(+)-pulegone on the central nervous system was evaluated. (R)-(+)-Pule- gone caused a signifi cant decrease in ambulation and an increase in pentobarbital-induced sleeping time in mice, indicating a central depressant effect. (+)-Pulegone also signifi cantly increased the latency of convulsions as assessed by the pentylenetetrazole (PTZ) method. The antinociceptive properties of this monoterpene were studied in chemical and thermal models of nociception. Chemical nociception induced in the fi rst and second phase of the subplantar formalin test was signifi cantly inhibited by (R)-(+)-pulegone and was not blocked by naloxone. Thermal nociception was also signifi cantly inhibited while (R)-(+)-pulegone increased the reaction latency of the mice in the hot plate test. These results suggest that (R)-(+)-pulegone is a psychoactive compound and has the profi le of an analgesic drug. Key words: Essential Oils, Antinociceptive Activity, Analgesic Introduction that they have the complex profi le of psychoac- tive drugs.
    [Show full text]
  • Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action
    molecules Review Medically Useful Plant Terpenoids: Biosynthesis, Occurrence, and Mechanism of Action Matthew E. Bergman 1 , Benjamin Davis 1 and Michael A. Phillips 1,2,* 1 Department of Cellular and Systems Biology, University of Toronto, Toronto, ON M5S 3G5, Canada; [email protected] (M.E.B.); [email protected] (B.D.) 2 Department of Biology, University of Toronto–Mississauga, Mississauga, ON L5L 1C6, Canada * Correspondence: [email protected]; Tel.: +1-905-569-4848 Academic Editors: Ewa Swiezewska, Liliana Surmacz and Bernhard Loll Received: 3 October 2019; Accepted: 30 October 2019; Published: 1 November 2019 Abstract: Specialized plant terpenoids have found fortuitous uses in medicine due to their evolutionary and biochemical selection for biological activity in animals. However, these highly functionalized natural products are produced through complex biosynthetic pathways for which we have a complete understanding in only a few cases. Here we review some of the most effective and promising plant terpenoids that are currently used in medicine and medical research and provide updates on their biosynthesis, natural occurrence, and mechanism of action in the body. This includes pharmacologically useful plastidic terpenoids such as p-menthane monoterpenoids, cannabinoids, paclitaxel (taxol®), and ingenol mebutate which are derived from the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, as well as cytosolic terpenoids such as thapsigargin and artemisinin produced through the mevalonate (MVA) pathway. We further provide a review of the MEP and MVA precursor pathways which supply the carbon skeletons for the downstream transformations yielding these medically significant natural products. Keywords: isoprenoids; plant natural products; terpenoid biosynthesis; medicinal plants; terpene synthases; cytochrome P450s 1.
    [Show full text]
  • Calamintha Nepeta (L.) Savi and Its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry
    molecules Review Calamintha nepeta (L.) Savi and its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry Mijat Božovi´c 1 and Rino Ragno 1,2,* 1 Rome Center for Molecular Design, Department of Drug Chemistry and Technology, Sapienza University, P.le Aldo Moro 5, 00185 Rome, Italy; [email protected] 2 Alchemical Dynamics s.r.l., 00125 Rome, Italy; alchemicalduynamics.com * Correspondence: [email protected]; Tel.: +39-06-4991-3937; Fax: +39-06-4991-3627 Academic Editor: Olga Tzakou Received: 28 December 2016; Accepted: 6 February 2017; Published: 14 February 2017 Abstract: Medicinal plants play an important role in the treatment of a wide range of diseases, even if their chemical constituents are not always completely recognized. Observations on their use and efficacy significantly contribute to the disclosure of their therapeutic properties. Calamintha nepeta (L.) Savi is an aromatic herb with a mint-oregano flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including antimicrobial, antioxidant and anti-inflammatory, as well as anti-ulcer and insecticidal properties. This study aims to review the scientific findings and research reported to date on Calamintha nepeta (L.) Savi that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, pulegone, the major chemical constituent of Calamintha nepeta (L.) Savi essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities.
    [Show full text]
  • Dr. Duke's Phytochemical and Ethnobotanical Databases List of Chemicals for Antibacterial
    Dr. Duke's Phytochemical and Ethnobotanical Databases List of Chemicals for Antibacterial Chemical Dosage (+)-8HYDROXYCALAMENENE -- (+)-8HYDROXYCALAMENENE -- (+)-GALBACIN -- (+)-T-CADINOL MIC=2-24 ug/ml (-)-EPICATECHIN -- (-)-EPIGALLOCATECHIN -- (-)-EPIGALLOCATECHIN-GALLATE -- (2Z,8Z)-10-ANGELOYLOXY-MATRICARIA-ESTER MIC=50->100 ug/mL (5R,8R,9S,10R)-12-OXO-ENT-3,13-CLERODIEN-15-OIC-ACID 20-25ug/ml (Z)-1,3-BIS(4-HYDROXYPHENYL)-1,4-PENTADIENE -- 1,2,4-TRIHYDROXYHEPTADECA-16-ENE -- 1,2-DIHYDROXY-4-GLUCOSYLNAPTHALENE -- 1,3,4,5-TETRACAFFEOYLQUINIC-ACID -- 1,3,5-TRIMETHOXYBENZENE -- 1,4-NAPTHAQUINONE -- 1,8-CINEOLE 50 ppm 1-ACETYLJATIVATRIOL -- 1-ETHYL-BETA-CARBOLINE -- 1-METHOXYCANTHIN-6-ONE -- 1-TULIPOSIDE-A -- 1-TULIPOSIDE-B -- 15-ALPHA-ACETOXYKAUREN-19-OIC-ACID 250 mM/ml 16-HYDROXYPSEUDOJOLKINOLIDE -- 2,4,6-TRIMETHOXYPHENOL -- 2,6-DIMETHOXY-P-BENZOQUINONE -- 2,7-DIHYDROXYCADALENE -- 2,7-DIMETHOXY-5-ISOPROPYL-3-METHYL-8,1-NAPTHALENE-CARBOLACTONE -- Chemical Dosage 2-HYDROXY-5-ISOPROPYL-7-METHOXY-3-METHYL-8,1-NAPTHALENE-CARBOLACTONE -- 3'-FORMYL-2',4',6'-TRIHYDROXY-5'-METHYLDIHYDROCHALCONE -- 3'-O-METHYLBATATASIN-III -- 3,4-DIHYDROXYBENZOIC-ACID -- 3,4-DIMETHOXYTOLUENE -- 3,4-SECOTRACHYLOBANOIC-ACID MIC=25 ug/ml 3,4-SECOTRITERPENE-ACID-20-EPI-KOETJAPIC-ACID MIC=3->6.25 ug/ml 3,5'-DIALLYL-2'-HYDROXY-4-METHOXYBIPHENYL-ETHER -- 3,5-BIS(3-METHYL-2-BUTENYL)-4-METHOXY-BENZOIC-ACID MIC=1.25->50 nM 3,5-DIMETHOXY-1,6-DIHYDROXYXANTHONE -- 3-CARBOMETHOXY-1,8-DIHYDROXYANTHRAQUINONE -- 3-METHOXY-1,8-DIHYDROXY-ANTHRQUINONE -- 3-O-METHYL-MANGOSTIN -- 3-OXO-11-ALPHA-HYDROXYOLEAN-12-ENE-30-OIC-ACID
    [Show full text]
  • Exploring Essential Oils As Prospective Therapy Against the Ravaging Coronavirus (SARS-Cov-2)
    IBEROAMERICAN JOURNAL OF MEDICINE 04 (2020) 322-330 Journal homepage: www.iberoamericanjm.tk Review Exploring essential oils as prospective therapy against the ravaging Coronavirus (SARS-CoV-2) Emmanuel Onah Ojaha,* aMedicinal Chemistry Research Group, Organic Chemistry Unit, Department of Chemistry, University of Ibadan, Ibadan, Nigeria ARTICLE INFO ABSTRACT Article history: Introduction: Aromatic plants produce diverse chemical constituents with potential to Received 09 June 2020 inhibit viral infections. These plants have been utilized for the prevention and treatment Received in revised form 16 June of a range of infectious and non-infectious diseases. Essential oils are among the plant- 2020 derived antiviral agents that are being employed in phytomedicine, and are considered as prospective drug candidate against the ravaging Coronavirus. Accepted 22 June 2020 Methods: Relevant articles relating to the concept were identified using a combination of manual library search as well as journal publication on the subject and critically Keywords: reviewed. Coronavirus Results: Essential oils in medicinal plants have extensive applications in medicinal Medicinal plants chemistry, aromatherapy and pharmaceuticals. Essential oils have several biological Essential oil properties such as antibacterial, antifungal, antiviral, antioxidant, anti-inflammatory, Aromatherapy wound-healing and anti-cancer effects in vitro and in vivo. Several reports have analyzed Antiviral and described essential oils as good antiviral agents against Respiratory tract viral infections hence are excellent prospective candidate against Corona virus. Conclusions: It is hoped that efficient and effective exploration and optimization of essential oils from medicinal plants would improve the drug discovery process against the ravaging Coronavirus. © 2020 The Authors. Published by Iberoamerican Journal of Medicine. This is an open access article under the CC BY license (http://creativecommons.
    [Show full text]
  • HMPC Public Statement on the Use of HMP Cont Pulegone, Menthofuran
    European Medicines Agency Evaluation of Medicines for Human Use London, 23 November 2005 Doc Ref: EMEA/HMPC/138386/2005 COMMITTEE ON HERBAL MEDICINAL PRODUCTS (HMPC) FINAL PUBLIC STATEMENT ON THE USE OF HERBAL MEDICINAL PRODUCTS CONTAINING PULEGONE AND MENTHOFURAN DISCUSSION IN THE HMPC January 2005 March 2005 RELEASE FOR CONSULTATION April 2005 DEADLINE FOR COMMENTS June 2005 REDISCUSSION IN HMPC November 2005 ADOPTION BY HMPC November 2005 The Committee on Herbal Medicinal Products endorses without any changes the ‘Position Paper on the use of herbal medicinal products containing pulegone and menthofuran’ adopted by the Herbal Medicinal Products Working Party (HMPWP) in May 2004. 7 Westferry Circus, Canary Wharf, London E14 4HB, UK Tel. (44-20) 74 18 84 00 Fax (44-20) 75 23 70 51 E-mail: [email protected] http://www.emea.eu.int ©EMEA 2005 Reproduction and/or distribution of this document is authorised for non commercial purposes only pdovided the EMEA is acknowledged Public Statement on the use of herbal medicinal products containing pulegone and menthofuran BACKGROUND Following the publication of the opinion of the Scientific Committee on Food (SCF) on pulegone and menthofuran the CPMP Herbal Medicinal Products Working Party has prepared this position paper reviewing the SCF opinion and recommending future action in relation to herbal medicinal products containing peppermint oil (Mentha piperita L.), mint oil (M. canadensis L., syn. M. arvensis var piperascens Malinv. Ex Holmes) and pennyroyal oil (M. Pulegium L. or Hedeoma pulegoides (L.) Pers). INTRODUCTION Peppermint oil (Ph Eur) contains maximum 4.0 % pulegone and between 1.0 and 9.0 % menthofuran.
    [Show full text]
  • Effective Antioxidant, Antimicrobial and Anticancer Activities of Essential Oils of Horticultural Aromatic Crops in Northern Egypt Hosam O
    Elansary et al. BMC Complementary and Alternative Medicine (2018) 18:214 https://doi.org/10.1186/s12906-018-2262-1 RESEARCHARTICLE Open Access Effective antioxidant, antimicrobial and anticancer activities of essential oils of horticultural aromatic crops in northern Egypt Hosam O. Elansary1,2,3* , Samir A. M. Abdelgaleil4, Eman A. Mahmoud5, Kowiyou Yessoufou3, Khalid Elhindi1 and Salah El-Hendawy1 Abstract Background: Identifying ornamental plants as new natural antioxidant and antimicrobial sources is always of great importance for the ornamental and horticultural industries. Methods: The antimicrobial activities of leaves and fruits peel essential oils of twelve ornamental and horticultural crops were determined by screening against wide spectrum of fungi and bacteria, and their respective in vitro antioxidant capacity was evaluated. Furthermore, the anticancer activities against several cancer cells, and one normal human cell line (HEK-293) were examined. Results: Origanum vulgare L. essential oil showed the best antioxidant, antibacterial and anticancer activities compared to screened crops by means of the DPPH and linoleic acid assays for antioxidants, MIC and MBC values for antibacterial activities and IC50 for antiproliferative activities. Such important activities in O. vulgare was attributed to high pulegone ratio (77.45%) as revealed by the GC/MS assay. Rosmarinus officinallis L. essential oil showed the highest antifungal activities by means of lowest MIC and MFC values which might be attributed to 1, 8-cineole (19.60%), camphor (17.01%) and α-pinene (15.12%). Conclusion: We suggest that oxygenated monoterpenes (i.e. linalool, terpinen-4-ol and pulegone) and monoterpene hydrocarbons play an important role in the essential oil antioxidant, antibacterial, antifungal and anticancer activities of diverse Egyptian ornamental and horticultural crops.
    [Show full text]
  • Calamintha Nepeta (L.) Savi and Its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry
    Review Calamintha nepeta (L.) Savi and its Main Essential Oil Constituent Pulegone: Biological Activities and Chemistry Mijat Božović 1 and Rino Ragno 1,2,* 1 Rome Center for Molecular Design, Department of Drug Chemistry and Technology, Sapienza University, P.le Aldo Moro 5, 00185 Rome, Italy; [email protected] 2 Alchemical Dynamics s.r.l., 00125 Rome, Italy; alchemicalduynamics.com * Correspondence: [email protected]; Tel.: +39-06-4991-3937; Fax: +39-06-4991-3627 Academic Editor: Olga Tzakou Received: 28 December 2016; Accepted: 6 February 2017; Published: 14 February 2017 Abstract: Medicinal plants play an important role in the treatment of a wide range of diseases, even if their chemical constituents are not always completely recognized. Observations on their use and efficacy significantly contribute to the disclosure of their therapeutic properties. Calamintha nepeta (L.) Savi is an aromatic herb with a mint-oregano flavor, used in the Mediterranean areas as a traditional medicine. It has an extensive range of biological activities, including antimicrobial, antioxidant and anti-inflammatory, as well as anti-ulcer and insecticidal properties. This study aims to review the scientific findings and research reported to date on Calamintha nepeta (L.) Savi that prove many of the remarkable various biological actions, effects and some uses of this species as a source of bioactive natural compounds. On the other hand, pulegone, the major chemical constituent of Calamintha nepeta (L.) Savi essential oil, has been reported to exhibit numerous bioactivities in cells and animals. Thus, this integrated overview also surveys and interprets the present knowledge of chemistry and analysis of this oxygenated monoterpene, as well as its beneficial bioactivities.
    [Show full text]
  • Antimicrobial and Physical Properties of Experimental Endodontic Sealers Containing Vegetable Extracts
    www.nature.com/scientificreports OPEN Antimicrobial and physical properties of experimental endodontic sealers containing vegetable extracts Daniela Coelho dos Santos 1, Andressa da Silva Barboza 2, Lara Rodrigues Schneider 1, Carlos Enrique Cuevas‑Suárez 3, Juliana Silva Ribeiro 2, Melissa Feres Damian 2, Angela Diniz Campos 4 & Rafael Guerra Lund 1,2,5* To assess the antimicrobial activity and the physical properties of resin‑based experimental endodontic sealers with the incorporation of vegetable extracts obtained from Bixa orellana, Mentha piperita, and Tagetes minuta species. The extracts were obtained and characterized by gas chromatography‑mass spectrometry (GC–MS), and minimum inhibitory concentration (MIC) against Streptococcus mutans, Enterococcus faecalis, and Candida albicans. The extracts were individually incorporated into a dual‑cure experimental sealer at a mass concentration of 0.5%. A commercial reference RealSeal was used. The sealers were evaluated by measuring the setting time, degree of conversion, dimensional stability, radiopacity, fow, and flm thickness of these materials, also and its antimicrobial efect was evaluated using the direct contact test. Data were statistically analyzed by analysis of variance and Tukey’s post‑hoc test at α = 0.05 signifcance level. The physical properties were not infuenced by the addition of the vegetable extracts (p > 0.05). For S. mutans, only T. minuta and B. orellana groups presented antibacterial activity after 24 h of contact (p < 0.05). All extracts evidenced an antibacterial efect against E. faecalis (p < 0.05). The experimental sealers hold promise as a novel vegetable sealer with great antimicrobial activity and also great physical–mechanical properties. Nonetheless, more studies are needed.
    [Show full text]