DOCSLIB.ORG

Biochemical Studies of Energy Production in the Failing Human Heart

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Biochemical Studies of Energy Production in the Failing Human Heart Biochemical studies of energy production in the failing human heart. C A Chidsey, … , P E Pool, A G Morrow J Clin Invest. 1966;45(1):40-50. https://doi.org/10.1172/JCI105322. Research Article Find the latest version: https://jci.me/105322/pdf Journal of Clinical Investigation Vol. 45, No. 1, 1966 Biochemical Studies of Energy Production in the Failing Human Heart * CHARLES A. CHIDSEY,t EUGENE C. WEINBACH, PETER E. POOL, AND ANDREW G. MORROW (From the Cardiology Branch and the Clinic of Surgery, National Heart Institute, and the Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Md.) The defective myocardial performance responsi- and in vivo (6, 7), and one clinical syndrome, char- ble for the syndrome of congestive heart failure has acterized by nonthyroid hypermetabolism and re- been considered for many years to involve a bio- sulting from uncoupling of these two processes, chemical abnormality. However, identification of has been identified by Luft and associates (8). this abnormality remains to be achieved. Muscle Mitochondrial function in the failing heart has cell function is basically a process by which chemi- previously been studied only in experimental ani- cal energy is transformed into the mechanical work mals, where the findings have been conflicting. of myofibrillar shortening and force development. Oxidative phosphorylation and the content of high The presence of a biochemical abnormality in the energy phosphate compounds have been reported failing cardiac muscle must therefore be sought to be normal in some of these studies (9-12), either in the mechanism of energy production or whereas in others defective energy production has in the mechanism of energy utilization by the con- been observed (13-17). Thus, it seemed evident tractile system. The present report is a study of that this problem, in the clinical syndrome of con- energy production in the failing human heart. gestive heart failure, could be solved definitively Considerable evidence has accumulated to indi- only by studying mitochondrial function and mor- cate that myocardial extraction of oxygen and of phology and the content of high energy phosphate oxidizable substrates is not diminished in patients compounds in human myocardial tissue. Accord- with congestive heart failure (1-3). These stud- ingly, the present study was carried out with myo- ies have not, however, provided evidence that the cardial tissue removed at the time of cardiac op- final supply of chemical energy to the contractile erations from 25 patients with congestive heart apparatus is normal. ATP, the immediate source failure. Mitochondria isolated from these tissues of chemical energy utilized by muscle (4), is were shown to possess a high degree of structural formed within the mitochondrion during the oxi- and functional integrity, and the myocardial con- dation of substrates by the process of oxidative tent of high energy phosphate compounds was not phosphorylation. The completeness with which found to be reduced. We concluded, therefore, the process of phosphorylation is coupled to that that there is no evidence for a biochemical abnor- of oxidation determines whether or not the energy mality of energy production in the failing human liberated in the oxidation of substrates becomes heart. available for useful mechanical work by the con- Methods tractile apparatus. A number of toxic agents have been shown to be capable of uncoupling electron This type of investigation on viable human cardiac tissue is now possible because of the development and ap- transport and phosphorylation, both in vitro (5) plication of effective surgical methods for prosthetic re- * Submitted for publication June 21, 1965; accepted placement of cardiac valves. Papillary muscles were re- September 23, 1965. moved from 14 patients with heart failure; in 13 patients, A partial report of this work was presented at a sec- muscles were obtained from the left ventricle at the time tional meeting of the American Society for Clinical In- of removal of diseased mitral valves, and in one patient vestigation, May 2, 1965. muscles were obtained from the right ventricle during t Address requests for reprints to Dr. Charles A. Chid- excision of the tricuspid valve. In all patients rheumatic sey, Dept. of Medicine, University of Colorado School of heart disease was responsible for the valvular deformity, Medicine, 4200 E. Ninth Ave., Denver, Colo. and all were on maintenance digoxin. The patients, seven 40 BIOENERGETICS IN HEART FAILURE 41 men and seven women, were 21 to 57 years of age. The contained the following in a final volume of 1.0 ml: major hemodynamic abnormality in ten patients was glycylglycine buffer (pH 7.4), 40 Amoles; potassium mitral regurgitation; in one patient, mitral stenosis; in phosphate (pH 7.4), 30 umoles; DPN, 1 /mole; cyto- one patient, tricuspid regurgitation; and in two patients, chrome c, 0.015 /mole; MgCl2, 5 /Lmoles; glucose, 25 combined aortic and mitral disease, necessitating replace- timoles; hexokinase (Sigma type III), 0.25 mg; and ment of both the aortic and mitral valves. Hemodynamic mitochondria, 0.25 ml. Incubation was for 20 minutes at evaluation by right and left heart catheterization was car- 30° C. Oxidative rates were determined manometrically, ried out in all 14 patients who comprised this study. To and the net uptake of phosphate was calculated from the represent nonfailing hearts, mitochondria also were pre- differences between initial and final phosphate concentra- pared from right ventricular outflow tract muscle re- tions (22). All incubations were done in duplicate and moved from two male patients with tetralogy of Fallot the average values reported. Determinations of oxygen during correction of this congenital defect. consumption were also carried out with the Clark elec- The general methods utilized in this clinic for mitral trode (23). Mitochondria (0.2 ml) were incubated at valve replacement have been previously described in de- 240 C in a sealed cuvette containing 1.5 ml of the follow- tail (18). The preoperative medications were pento- ing: glycylglycine buffer (pH 7.4), 30 Amoles; potas- barbital, atropine, and meperidine. Anesthesia was in- sium phosphate (pH 7.4), 5 Mmoles; KCl, 180 Amoles; duced with thiopental and maintained with halothane in MgCl2, 12 /moles; and substrate, 10 Amoles. Additions a concentration of 0.5 to 1.0%. The mitral valve was to this medium are indicated in the Figures. Respira- exposed during total cardiopulmonary bypass, and after tory control ratios were determined manometrically and the valve leaflets had been detached from the anulus, the polarographically by dividing the oxidative rates in the papillary muscles were divided from the ventricular wall presence of ADP acceptor by the rate measure when the at their origins and the valve and muscles removed en ADP acceptor was absent (24). ATPase activity was bloc. The patients' temperatures were usually 340 to assayed (21) in a medium containing 40 Emoles glycyl- 350 C, and bypass had been in progress for approximately glycine buffer (pH 7.4), 6 /4moles ATP, 0.2 ml of mito- 10 to 15 minutes when the papillary muscles were tran- chondria, and sufficient 0.25 M sucrose to make a final sected. Immediately upon removal of the specimen, it was volume of 1 ml; additions are as indicated in Table III. placed on ice, dissected, and muscle tissue obtained that Incubations were carried out at 30° C for 10 minutes. was free of fibrous connections to the valve. Measurement of high energy phosphate compounds was Muscle specimens weighing 0.9 to 4.0 g were used for made in biopsies of the myocardium taken from 14 pa- the isolation of mitochondria by a modification of the tients with heart failure in whom replacement of the method of Hatefi, Jurtshuk, and Haavik (19). All tis- mitral or aortic valve or both was performed. In all sues and materials were maintained at 00 C. Tissue patients rheumatic heart disease was responsible for the specimens were pressed through a stainless steel disc valvular deformity, and all were on maintenance digoxin. with 0.5-mm perforations, and this tissue mince was sus- The patients were 21 to 53 years of age; nine were males pended in 4 to 6 vol of 0.25 M sucrose containing ATP, and five females. The major hemodynamic abnormality MgCl2, succinate, and Tris-HCl (pH 7.8) each in a con- in six patients was mitral regurgitation; in one patient, centration of 10' mole per L. Nagarse proteinase 1 was aortic regurgitation; in one patient, mitral stenosis; in added to a concentration of 1 mg per ml and the mixture six patients, aortic stenosis; and in one patient aortic incubated in an ice bath for 20 minutes and intermittently stenosis was combined with mitral valve disease, neces- stirred with a magnetic stirring bar. The tissue mince sitating replacement of both valves. For comparison, was transferred to a smooth-walled homogenizing vessel biopsies also were taken at the time of cardiac operation and disrupted with a loose-fitting motor-driven Teflon from eight patients without previous histories of heart pestle with 15 strokes. The homogenate was centrifuged failure who were undergoing repair of septal defects or in a refrigerated centrifuge at 1,000 X g (mean force) pultnonic stenosis. Biopsies were taken from the right for 10 minutes. The supernatant fluid was decanted and ventricle in the nonfailure group and from the left ven- centrifuged again at 25,000 X g for 10 minutes to obtain tricle in the failure group 2 to 10 minutes after the in- the mitochondrial pellet. This was washed with 0.25 M stitution of total cardiopulmonary bypass. In all pa- sucrose, recentrifuged at 25,000 X g for 10 minutes, and tients body temperatures were 340 to 350 C, and the resuspended in sufficient 0.25 M sucrose to make a sus- coronary circulation was intact.
Load more

Recommended publications
  • Programmed Cell Death and Lysis in Bacteria and the Benefits to Survival
    Programmed Cell Death and Lysis in Bacteria and the Benefits for Survival. Mirjam Boonstra Rijksuniversiteit Groningen 2009 Teachers: Prof. Dr. O.P. Kuipers & Dr. A.T. Kovacs 1 Introduction Bacterial cell death is an interesting phenomenon because it plays a crucial role in many important processes. Bacterial cell death does not just occur in the late stationary phase when nutrient limitation can lead to starvation. Bacteria are capable of initiating autolysis during certain conditions, such as biofilm formation, competence, sporulation and stress response. One form of lysis is autolysis, bacterial suicide. Autolysis in bacteria constitutes programmed cell death (PCD) because death of the cell is the result of an internal mechanism that can be activated by internal or external signals. Another cause of death of a subpopulation of cells is allolysis; allolysis is the killing of sibling cells by bacteria. There are many different mechanisms that can cause lysis in bacteria. Which mechanisms are used depends on the species of bacteria and the circumstances. The possible benefits to killing sibling cells are obvious; killing other cells releases their nutrients into the environment. But what are the benefits to killing yourself? The answer to this lies in the complex nature of bacterial communities. Bacteria are for instance, capable of forming biofilms in which there exists a spatial and temporal difference in gene expression of genetically identical bacteria. The difference in gene expression patterns plays an important role in deciding which cells undergo autolysis. Bacteria also undergo complex developmental processes such as sporulation and competence in which autolysis and allolysis play a crucial role.
    [Show full text]
  • Towards Accelerated Autolysis? Dynamics of Phenolics, Proteins, Amino Acids and Lipids in Response to Novel Treatments and During Ageing of Sparkling Wine
    beverages Article Towards Accelerated Autolysis? Dynamics of Phenolics, Proteins, Amino Acids and Lipids in Response to Novel Treatments and during Ageing of Sparkling Wine Gail B. Gnoinski 1,*, Dugald C. Close 1, Simon A. Schmidt 2 and Fiona L. Kerslake 1 1 Horticulture Centre, Tasmanian Institute of Agriculture, University of Tasmania, Sandy Bay, Hobart 7005, Australia; [email protected] (D.C.C.); fi[email protected] (F.L.K.) 2 The Australian Wine Research Institute, Glen Osmond, Adelaide 5064, Australia; [email protected] * Correspondence: [email protected] Abstract: Premium sparkling wine produced by the traditional method (analogous to the French méthode champenoise) is characterised by the development of aged wine character as a result of a second fermentation in the bottle with lees contact and lengthy ageing. Treatments (microwave, ultrasound, or β-glucanase enzymes) were applied to disrupt the cell wall of Saccharomyces cerevisiae and added to the tirage liquor for the second fermentation of Chardonnay-Pinot Noir base wine cuvée and compared to a control, to assess effects on the release of phenolics, proteins, amino acids, and lipids at 6, 12 and 18 months post-tirage. General responses to wine ageing included a 60% Citation: Gnoinski, G.B.; Close, D.C.; increase in the total phenolic content of older sparkling wines relative to younger wines and an Schmidt, S.A.; Kerslake, F.L. Towards increase in protein concentration from 6 to 12 months bottle age. Microwave and β-glucanase enzyme Accelerated Autolysis? Dynamics of treatments of yeast during tirage preparation were associated with a 10% increase in total free amino Phenolics, Proteins, Amino Acids and acid concentration and a 10% increase in proline concentration at 18 months bottle age, compared to Lipids in Response to Novel control and ultrasound treatment.
    [Show full text]
  • Degradation of Lipids in Yeast (Saccharomyces Cerevisiae) at the Early Phase of Organic Solvent-Induced Autolysis
    Agric. Biol. Chem., 42 (2), 247•`251, 1978 Degradation of Lipids in Yeast (Saccharomyces cerevisiae) at the Early Phase of Organic Solvent-induced Autolysis MichikoISHIDA-ICHIMASA Departmentof Biology, Faculty of Science,Ibaraki University , Bunkyo,Mito, Japan ReceivedMay 30, 1977 Initial stage of organic solvent-induced autolysis in yeast was studied with 14C-acetate labeled cells. In the case of toluene-induced autolysis, primary cell injury which was estimated by leakage of UV absorbing substances from cell accompanied rapid deacylation of phos pholipids. Lysophospholipids did not occur during autolysis. When autolysis was induced by addition of ethyl acetate, phospholipids of yeast cells were not degraded so much. Ethyl acetate rather inhibited yeast phospholipase activity under the condition tested. Several organic solvents have been used to Extraction and analysis of lipids. The incubated mixtures were heated for 10 min in boiling water and initiate autolysis in yeast for the isolation of sonicated twice for 5 min at 20 KC in an ice bath. enzymes and other cell constituents. Some Then, lipids were extracted from the aqueous suspen examples are the applications of chloroform or sion by the procedure described by Bligh and Dyer.7) ethanol, toluene and ethyl acetate in the prepa Appropriate amounts of extracted lipids were chro rations of proteases,l) ƒÀ-fructofuranosidase,2,3) matographed on precoated thin-layer plates of silica gel 60 (without fluorescent indicator) (E. Merck Co.) with and cytochrome c,4) respectively. Although it solvents of petroleum ether-diethyl ether-acetic acid is well known that some organic solvents such (80: 30: 1, by vol8)) for neutral lipids, and chloroform- as diethyl ether and ethanol stimulate enzy methanol-acetic acid-water (25: 15: 4: 1, by vol.) for matic degradation of phospholipids, the me phospholipids.
    [Show full text]
  • Chemical and Cytological Changes During
    CHEMICAL AND CYTOLOGICAL CHANGES DURING THE AUTOLYSIS OF YEASTS A thesis submitted to The University of New South Wales as fulfilment of the requirements for the degree of DOCTOR OF PHILOSOPHY by Tatang Hernawan Sarjana Farmasi, Apoteker (I T B, Indonesia) Master of Applied Science (UN SW, Australia) Department of Food Science and Technology The University of New South Wales Kensington, N. S. W. Australia February 1992 D E C L A R A T I O N The candidate, Tatang Hernawan, hereby declares that this thesis is his own work and that, to the best of his knowledge and belief, it contains no material previously published or written by another person nor material which to substantial extent has been accepted for the award of any degree or diploma of a university or other institute of higher learning, except where due acknowledgement is made in the text of the thesis. T a t a n g H e r n a w a n A C K N O W L E D G E M E N T S I wish to express my sincere gratitude to Associate Professor G. H. Fleet, Department of Food Science and Technology, University of New South Wales, Australia, as my supervisor for his guidance, advice and encouragement during this project and thesis production. I would like to acknowledge the assistance of laboratory staff of the Department of Food Science and Technology, Mr P. Mark, of the Electron Microscopy Unit, and Ir E. Poerwanto, a fellow postgraduate student, University of New South Wales. I would like to thank fell ow students in the Department of Food Science and Technology for a friendly situation throughout this study and Ms H.
    [Show full text]
  • Novel Sparkling Winemaking Technologies and Visualising Yeast Autolysis
    Novel sparkling winemaking technologies and visualising yeast autolysis Gail Gnoinski, Fiona Kerslake, Dugald Close, Bob Dambergs and Simon Schmidt 1 Background − Complexity in sparkling wine is derived from viticultural practices, base wine composition, winemaking practices and wine maturation (Jones et al., 2014; Kerslake et al., 2013) − Sensory cues not well understood, distinguished or agreed upon − Wine Australia priority to identify important compounds contributing to flavour, mouth feel and texture of sparkling wine character Autolysis − Enzymatic degradation (hydrolysis) of yeast cell constituents after cell death Yeast cell structure − Cell wall mannoprotein, glucan − Amino acids, Proteins, Peptides Wine quality − Yeast cellular components released in wine − Autolytic character develops slowly Scientific research − Changes occur in the lees structure as wine ages 1 13 month months (Tudela et al. 2012) Research question & objectives − Can alternative methods be implemented to artificially induce yeast lysis in sparkling winemaking? − Application of novel technologies (ultrasound, microwave, enzymes) to break down yeast cells − Shorter ageing period on lees with developed characteristics for earlier release − Visualise cell level impact associated with novel-treated lees Tasting wine produced using novel technologies − Participants taste the five different sparkling wines presented − Make some notes to record impressions of the wines Industry trial application − Hill-Smith Family Vineyards Yalumba, Adelaide − Saccharomyces cerevisiae
    [Show full text]
  • Dissolved ATP Turnover in the Bransfield Strait, Antarctica During a Spring Bloom
    MARINE ECOLOGY PROGRESS SERIES Vol. 57: 35-44. 1989 Published September 15 Mar. Ecol. Prog. Ser. Dissolved ATP turnover in the Bransfield Strait, Antarctica during a spring bloom Michele P. ~awrocki',David M. Karll, 2v* Department of Oceanography, University of Hawaii, Honolulu, Hawaii 96822, USA Division of Oceanic Biology, Hawaii Institute of Geophysics, University of Hawaii, Honolulu, Hawaii 96822. USA ABSTRACT- Ambient concentrations and turnover rates of dissolved adenosine triphosphate (D-ATP) were measured at 5 stations in the Bransfield Strait, Antarctica during the 1986-87 Research on Antarctic Coastal Ecosystem Rates (RACER) field program. The study area was pre-selected to include several different coastal and oceanic habitats expected to vary considerably in timing and magnitude of the annual spring phytoplankton bloom. D-ATP concentrations varied both spatially and temporally during the 4 mo of observation. Dunng the initial stages of bloom development, there was a signif~cant positive correlation between the concentration of particulate (P) ATP (i.e. biomass) and D-ATP; how- ever, this relationship deteriorated later in the season. At the height of the spring bloom (January), we observed a D-ATP concentration gradient in excess of an order of magnitude for our coastal to open ocean transect. Dissolved ATP fluxes (uptake rate times ambient concentration; ng I-' d-l) were highly correlated with D-ATP concentrations, indicating that bloom conditions stimulated both production and removal processes. We assessed the potential role of 3 independent processes as a source of D-ATP in Antarctic coastal waters: excret~on/exudation,production during micro- or macrozooplankton grazing and cell death/autolysis.
    [Show full text]
  • Development of a New, Innovative, Specific Yeast Autolysate to Improve the Quality of Red Wine
    Development of a new, innovative, specific yeast autolysate to improve the quality of red wine Anthony Silvano1, Jose-Maria Heras1, Julie Mekoue-Nguela1,2, Marion Schiavone1,3, Nathalie Sieczkowski1 1 Lallemand SAS, 19, Rue des briquetiers, 31702 Blagnac, France 2 UMR 1083 Sciences pour l’Œnologie, INRA, Montpellier SupAgro, Université de Montpellier, 34060 Montpellier, France 3 LISBP, Université de Toulouse, CNRS, INRA, INSA, 135 avenue de Rangueil, 31077 Toulouse, France Introduction Consumer demand for fruity red wines with intense colour and good mouthfeel continues to grow. Aging on lees is a widespread traditional winemaking technique aimed in part at reducing astringency and bitterness while increasing body and aromatic length and complexity. Aging on lees can also help stabilize the colour of red wines. During this step, winemakers reap the many well known benefits—including the release of mannoproteins—provided by adding dead or dying autolyzed yeast (Rodriguez et al., 2005). To avoid the inconvenience of traditional aging on lees, a practice has developed over the past 15 years where specific inactivated yeasts are added to promote the release of polysaccharides (Guadalupe et al., 2007, and Rodriguez-Bencomo et al., 2010). Recent scientific advances have provided more precise tools for characterizing wine yeasts and their products, leading to the development of a new yeast autolysate (MEX-WY1) with unique properties based on an innovative combination of a specific inactivation process and a special strain of Saccharomyces cerevisiae. A better understanding of how products derived from yeasts interact with polyphenols in red wine production The idea that certain polysaccharides can bind with tannins and thereby reduce the astringency of wines has been around for a number of years.
    [Show full text]
  • Detection of Protein Markers for Autophagy, Autolysis and Apoptosis Processes in A
    bioRxiv preprint doi: https://doi.org/10.1101/324772; this version posted May 18, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Detection of protein markers for autophagy, autolysis and apoptosis processes in a 2 Saccharomyces cerevisiae wine flor yeast strain when forming biofilm 3 4 Jaime Moreno-Garciaa, Juan Carlos Mauricioa#, Juan Morenob and Teresa Garcia- 5 Martineza 6 7 aDepartment of Microbiology, Agrifood Campus of International Excellence ceiA3, 8 University of Cordoba, Cordoba, Spain. 9 bDepartment of Agricultural Chemistry, Agrifood Campus of International Excellence 10 ceiA3, University of Cordoba, Cordoba, Spain. 11 12 Running tittle: Autophagy/autolysis/apoptosis proteins in flor biofilm 13 14 #Address correspondence to Juan Carlos Mauricio, [email protected]. 15 *Present address: Jaime Moreno-García, Department of Chemistry, Umeå University, 16 SE-901 87 Umeå, Sweden. 17 18 19 20 21 bioRxiv preprint doi: https://doi.org/10.1101/324772; this version posted May 18, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 22 Abstract 23 Yeast autophagy, autolysis and apoptosis are triggered by nutrient starvation 24 conditions that usually take place in winemaking. Biological aging of Sherry wines 25 constitutes an enological environment suitable for the induction of these biological 26 processes due to the scarcity of nutrients and formation of yeast social communities, i.e. 27 biofilm; however, few studies have been carried out in this regard.
    [Show full text]
  • Subject: Zoology Cell Biology Name of the Topic : Lysosomes Lysosomes
    Subject: Zoology Cell Biology Name of the Topic : Lysosomes Lysosomes Animal cells have another set of organelles not found in plant cells i.e lysosomes. Lysosomes were initially named as ‘perinuclear dense bodies’. The name ‘lysosome’ was coined by C.De Duve in 1955. Lysosomes occur in all animal cells. However, they are not found in mature mammalian erythrocytes. Muscle cells contain very few lysosomes. They are numerous in epithelial cells of secretory and excretory organs. The lysosomes are so called because they contain lytic or destructive enzymes. Since most of the lysosomal enzymes function better under acidic conditions, they are collectively called as Acidic hydrolases. Enzymes within the lysosomes aid the breakdown of proteins, polysaccharides, lipids, nucleic acids, and even worn-out organelles. These enzymes are active at a much lower pH than that of the cytoplasm. Therefore, the pH within lysosomes is more acidic than the pH of the cytoplasm. Lysosomes are involved in intracellular digestion and are primarily meant for destroying unwanted and aged organelles inside the cells. The lysosomes are the cell’s “garbage disposal.” Recent studies reveal that lysosomes may contain up to 40 hydrolytic enzymes. The enzymes are mostly proteases, nucleases, glycosidases, lipases, phospholipases, phosphatases, and sulphatases. All the enzymes do not occur in the same lysosome but there are different sets of enzymes in different types of lysosomes. Because of the presence of these digestive enzymes, lysosomes are called “Suicidal bags”. Another interesting property of lysosomes, isolated from kidney cells is their ability to trap and concentrate the foreign proteins injected, suggesting that these may help in digestion or detoxification of foreign materials by pinocytosis.
    [Show full text]
  • Programmed Cell Death During Plant Growth and Development
    Cell Death and Differentiation (1997) 4, 649 ± 661 1997 Stockton Press All rights reserved 13509047/97 $12.00 Review Programmed cell death during plant growth and development Eric P. Beers tracheary element and sclereid differentiation, sieve element differentiation, and leaf and flower petal senescence. Due to Department of Horticulture, Virginia Polytechnic Institute and State University, the broad range of topics covered, it was not possible to Blacksburg, Virginia 24061-0327, USA present in this space comprehensive reviews of each subject. Rather, to examine whether apoptosis is a universal pathway Received 24.2.97; revised 30.6.97; accepted 14.7.97 to pcd in plants, I have reviewed reports describing Edited by B.A. Osborne morphology of cells undergoing pcd during the processes listed above. Information concerning potential biochemical and molecular markers for plant pcd is also reviewed, with Abstract special attention to possible roles for proteases, since This review describes programmed cell death as it signifies research in this area has established proteases as important the terminal differentiation of cells in anthers, xylem, the mediators of pcd in animals. suspensor and senescing leaves and petals. Also described There is continuing interest in whether apoptosis occurs are cell suicide programs initiated by stress (e.g., hypoxia- in plants. Electron microscopy has revealed that during apoptosis in animals, chromatin condenses and segregates induced aerenchyma formation) and those that depend on into sharply delineated masses positioned at the nuclear communication between neighboring cells, as observed for envelope (Ellis et al, 1991; Kerr and Harmon, 1991). incompatible pollen tubes, the suspensor and synergids in Condensed chromatin is electron dense and is often some species.
    [Show full text]
  • Differential Proteomics Study of Postharvest Volvariella Volvacea
    www.nature.com/scientificreports OPEN Diferential proteomics study of postharvest Volvariella volvacea during storage at 4 °C Lei Zha1,2, Mingjie Chen1,2, Changxia Yu1, Qian Guo1, Xu Zhao 1, Zhengpeng Li1, Yan Zhao1*, Chuanhua Li1 & Huanling Yang1 The postharvest storage of Volvariella volvacea is an important factor limiting the industry development. Low-temperature storage is the traditional storage method used for most edible fungi, but V. volvacea undergoes autolysis at low temperature. To understand the molecular mechanism underlying the low-temperature autolysis of V. volvacea after harvesting, fruiting bodies of V. volvacea strain V23 were stored at 4 °C. Based on our previous study, in which the changes of morphological and physiological indexes during storage for 0, 6, 12, 24, 30, 36, 48 and 60 h were measured; four time points, namely, 0, 12, 24 and 60 h, were selected for this diferential proteomics study. The proteomic changes in the postharvest storage samples were studied by isobaric tags for relative and absolute quantifcation-coupled two-dimensional liquid chromatography-tandem mass spectrometry (2D LC–MS/MS). A total of 2,063 proteins were identifed, and 192 diferentially expressed proteins (DEPs), including 24 up-regulated proteins and 168 down-regulated proteins, were detected after 12 h of storage. After 24 h of storage, 234 DEPs, including 48 up-regulated and 186 down-regulated proteins, were observed, and after 60 h, 415 DEPs, including 65 up-regulated proteins and 350 down-regulated proteins, were observed. An in-depth data analysis showed that the DEPs participated in various cellular processes, particularly metabolic processes.
    [Show full text]
  • Insights Into Plant Programmed Cell Death Induced by Heavy Metals—Discovering a Terra Incognita
    cells Review Insights into Plant Programmed Cell Death Induced by Heavy Metals—Discovering a Terra Incognita Klaudia Sychta * , Aneta Słomka and Elzbieta˙ Kuta Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 9 Gronostajowa Str., 30-387 Kraków, Poland; [email protected] (A.S.); [email protected] (E.K.) * Correspondence: [email protected]; Tel.: +48-12-664-60-30 Abstract: Programmed cell death (PCD) is a process that plays a fundamental role in plant devel- opment and responses to biotic and abiotic stresses. Knowledge of plant PCD mechanisms is still very scarce and is incomparable to the large number of studies on PCD mechanisms in animals. Quick and accurate assays, e.g., the TUNEL assay, comet assay, and analysis of caspase-like enzyme activity, enable the differentiation of PCD from necrosis. Two main types of plant PCD, developmen- tal (dPCD) regulated by internal factors, and environmental (ePCD) induced by external stimuli, are distinguished based on the differences in the expression of the conserved PCD-inducing genes. Abiotic stress factors, including heavy metals, induce necrosis or ePCD. Heavy metals induce PCD by triggering oxidative stress via reactive oxygen species (ROS) overproduction. ROS that are mainly produced by mitochondria modulate phytotoxicity mechanisms induced by heavy metals. Complex crosstalk between ROS, hormones (ethylene), nitric oxide (NO), and calcium ions evokes PCD, with proteases with caspase-like activity executing PCD in plant cells exposed to heavy metals. This pathway leads to very similar cytological hallmarks of heavy metal induced PCD to PCD induced by other abiotic factors.
    [Show full text]