Human CIRBP (1-172, His-Tag) - Purified

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AR09973PU-N Human CIRBP (1-172, His-tag) - Purified

Alternate names: A18HNRNP, CIRP, Cold-inducible RNA-binding protein, Glycine-rich RNA-binding protein CIRP Quantity: 0.1 mg Concentration: 0.5 mg/ml (determined by Bradford assay) Background: Cold inducible RNA-binding protein, also known as CIRBP, plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. It is localizes to the nucleus and contains one RRM (RNA recognition motif) domain. Expressed ubiquitously, CIRBP is thought to play an essential role in the suppression of cellular proliferation in response to UV irradiation or extreme cold. Uniprot ID: Q14011 NCBI: NP_001271 GeneID: 1153 Species: Human Source: E. coli Format: State: Liquid purified protein Purity: >95% by SDS-PAGE Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50% glycerol, 0.2M NaCl Description: Recombinant human CIRBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASDEGKLFV GGLSFDTNEQ SLEQVFSKYG QISEVVVVKD RETQRSRGFG FVTFENIDDA KDAMMAMNGK SVDGRQIRVD QAGKSSDNRS RGYRGGSAGG RGFFRGGRGR GRGFSRGGGD RGYGGNRFES RSGGYGGSRD YYSSRSQSGG YSDRSSGGSY RDSYDSYATH NE Molecular weight: 20.8 kDa (192aa) confirmed by MALDI-TOF Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Sheikh MS, Carrier F, Papathanasiou MA, Hollander MC, Zhan Q, Yu K, et al. Identification of several human homologs of hamster DNA damage-inducible transcripts. Cloning and characterization of a novel UV-inducible cDNA that codes for a putative RNA-binding protein. J Biol Chem. 1997 Oct 17;272(42):26720-6. PubMed PMID: 9334257. 2. Nishiyama H, Higashitsuji H, Yokoi H, Itoh K, Danno S, Matsuda T, et al. Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene. Gene. 1997 Dec 19;204(1-2):115-20. PubMed PMID: 9434172.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20131104 1 / 2 AR09973PU-N: Human CIRBP (1-172, His-tag) - Purified

Pictures: Recombinant human CIRBP, 1-172 aa, His- tagged

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20131104 2 / 2