Microbiological Control

Table of Contents

Microbiological Quality Control 4 Microsart® @filter 100 and 250 33 Air Monitoring Microsart® Funnel 100 and 250 35 ® MD8 airscan 6 Biosart® 250 Funnels 37 AirPort MD8 7 Combisart® Systems 38 Gelatine Membranes 8 Microsart® Combi.jet 42 BACTair™ - Big Impact 9 How to Set Up a Vacuum Accessories for MD8 devices 10 Filtration System 44 Colony Counting Traditional Filter Holders 47 Gridded Membranes 12 Accessories 50 ® Microsart e.motion 14 School Kit 58 Gridded Membranes 16 Sterility Testing Membranes without Grid 20 Sterisart® Universal Pump 59 Hydrophobic Edged Membranes 22 Sterisart® NF 60 Nutrient Pad Sets 24 Reusable System 64 Culture Media and Absorbent Pads 28 Services and Trainings ® Biosart® 100 Monitors 30 CONFIDENCE Validation Services 66 ® Biosart® 100 Nutrient Media 32 EXPAND Trainings and Seminars 67 Chemical Compatibility 68

3 Microbiological Quality Control 4 developed and sterile packaging that does membranes from their individual, specially membrane filter The fully automated Microsart – – – – Microsart to conventional products that's it where handling on less working area in comparison terized by innovation and clever design. Simple biological analysis, which are especially charac the most recent products from SSB for micro- The Microsart innovative colony counting Microsart for colony growth. are ready to use and strictly quality controlled membrane filters for this application. These factures Sartorius Stedim Biotech specifically manu- sample volume. are then counted and related to the filtered incubation on the membrane filter surface brane filter. The colonies that develop during place through the pore system of the mem- exchange of nutrients and metabolites takes detect these microbes. During incubation, the priate culture medium and incubated to membrane filter is then placed on an appro- on the surface of the membrane filter. The the sample are retained by the pore structure is filtered. Any microorganisms present in size is placed in a filter holder and the sample membrane filter with the appropriate pore In the standard membrane filter method, a equipment to carry out this method. Biotech offers the ideal range of filters and these requirements, and Sartorius Stedim The membrane filter method optimally meets a practical microbiological testing method. conditions are the requirements placed on effective testing procedures under routine the capability of performing efficient, cost- trace contamination or infection as well as Quantitative and reproducible detection of Colony counting Microbiological Quality Control in design The hardware is intelligent and functional All consumables are ready-to-use Easy funnel removal Easy membrane removal individually packaged gridded ® products have always stood out. ® – The product family for ® product family consists of all Microsart Page Nutrient Pad Sets: Page Membrane filter dispenser releases gridded 24 14 ® e.motion ® e.motion dispenser:

- by Sartorius Stedim Biotech. nation – that's advanced colony counting but minimizes the risk of secondary contami- @filter not only saves time and labor costs @vance promising their level of safety. Microsart flows and increase efficiency without com- are driven by the need to optimize their work The pharmaceutical and biotech industries becoming an increasingly costly business. and bringing new drugs to the market is The process of producing pharmaceuticals funnel and gridded membrane in one unit. 250 filter units, a ready-to-use combination launched with the Microsart Microsart environmentally friendly manner. The new ready-to-use and can be disposed of in an products, these products are delivered sterile, Following the trend of using single-use safety and thus more reliable results. progress and intelligent design, enhanced ceutical and biotech industry: Microsart microbiological applications in the pharma- Microsart A new product line within the successful of Pharma-Biotech products Advance your microbiological control Microsart compliance with ISO 7704. flow versions are tested and released in 0.45 µm membrane filters, the special high Just like every lot of Sartorius Stedim Biotech growth promotion on High Flow Membranes. and throughput. thanks to their high flow rate performance size membranes enable faster filtration runs designed pore structures of 0.45 µm pore 0.45 µm pore size membranes. The specially flow rates compared with conventional flow membranes. They deliver 30% higher special membrane versions known as Sartorius Stedim Biotech additionally supplies biological analysis. size are used on a standard basis for micro- lot number. Membranes with a 0.45 µm pore labeled with the product identification and easy-to-open envelopes. Each one is clearly ally, sterile-packaged membrane filters in Sartorius Stedim Biotech also offers individu- not require any interleaving paper. Moreover, ® . @vance ® ® ® @vance product family is aimed directly at @vance Page Microsart Page gridded membrane filters: Individually packaged, Especially E. coli shows best ® ® stands for even more product line is being ® 33 12 – ® @filter:

@filter 100 and high-

Page membrane filters: Gelatin 8

used for quantitative sampling of airborne Gelatin membrane filters are routinely Airborne bacteria and viruses deliver excellent results. ether sulfone membrane (100,000 MWCO) Sartocon For isolation of bacteriophages from water, or 0.45 µm. (diameter 142 mm), of the pore size 0.2 µm organisms specifies polyamide membranes A procedure for collecting Legionella Other bacteriological water tests and reproducible results for up to 24 months. the globe assure such consistently high quality No other ready-to-use Stedim Biotech Nutrient Pads Sets so unique: And this is precisely what makes the Sartorius up to 24 months. our As a result, it guarantees the high quality of perature during transportation and storage. Pad from fluctuations in humidity and tem- sensitive formula constituents of the Nutrient This special packaging in bags protects the petri dishes are sealed in an aluminum bag. petri dish and sterilized. Ten each of these each of which is individually inserted in a NPS box contains 100 sterile Nutrient Pads, Pads in a new packaging design. The standard the new to changing application requirements. Besides development program to adapt our products NPS are continuously enhanced as part of our as for water analysis. try and in the pharmaceutical industry as well types needed in the food and beverage indus- Our wide array of grow on which ately. To find out which colonies typically sterile water, they are ready to use immedi- moistened with 3.0–3.5 ml of demineralized, petri dish and sterilized. After they have been that are already individually inserted in a venience. These are dehydrated Nutrient Pad Sets (NPS) provide added con- NPS throughout their entire shelf life NPS types, we also offer Nutrient cross-flow filter cassettes with poly - NPS, please refer to page culture media covers all the culture media around Biosart Page Biosart Page 30 37 culture media ® ® 100 Monitors: 250 Funnels:

24.

Biosart steel funnels and vacuum filters Monitors can be used in place of Sterile of samples For faster and more convenient filtration ambient air of dairies. routine monitoring of bacteriophages in the Gelatin membrane filters can also be used for identification of the type of virus (page buffer or a different medium for subsequent the viruses collected and can be dissolved in Gelatin appears to have a protective effect on bacteriophages has been proven. The reason: collecting the smallest airborne viruses and toring. In addition, their effectiveness in microbes in cleanroom and isolator moni- Biosart (page base ensures exceptionally fast filtration runs next. The large inner diameter of the funnel consuming sterilization of one sample to the Sterisart sterile venting. the multi-branch manifold has an air filter for to meet specific needs. Each filter station on and consumables to be optimally combined The Combisart The 250-ml Biosart according to international pharmacopeias. performed in a completely closed system Combisart dish (page Monitor can be easily converted into a petri interior cellulose pad, the lid and base of the appropriate liquid culture medium to wet the various culture media. After pouring in an units need to be used in conjunction with colors and diameters. The completely sterile ters in a choice of different pore sizes, filter capacity are available with incorporated fil- These complete Monitors featuring a 100-ml Sterisart 37). single-use funnels and ® ® 250 Funnels 100 Monitors ® ® enables sterility testing to be ® 30). Systems ® design enables equipment ® funnels eliminate time- Sterisart Page Page Combisart 38 59 preassembled ® ® holders. stainless

8). 5

Microbiological Quality Control Air Monitoring | MD8 airscan® 6 the air sampler for remote sampling. The filter unit can be placed separately from The exceptionally high air flow rate of 8 m GMP”), isolators, or blow-fill-seal machines. (classification according to “EU Guide for at filling lines in sterile areas of class A tive detection of air-borne organisms, mainly The system is routinely used for the quantita- sampler and disposable gelatine filter units. Accessories for the Each version can be switched from 50 to 60 Hz and back. 16747 Special brochures available on request. Order no. SMI2001-e|SM-3011-e 17528--80----VPD Air flow rate 17528--80----BZD 17528--80----ACD Order number Disposable gelatine units, sterile, pack of 10 Ordering information for consumables 17801 Order number Specifications for the Specifications Air Sampler for Critical Applications The system consists of the Correction of the air flow Dimensions (L Weight Noise level Max. deviation Timer 16746 Order number of 1 m are usual in laminar flow as well as filtration enables isokinetic sampling at flow rates that Ordering information for the Inclusive filter holder rate setting 3 air very quickly (less than 8 minutes). + W +

MD8 airscan MD8 airscan

MD8 airscan MD8 airscan MD8 airscan but label on innermost bag Individually packed in 3 polyethylene bags each, Individually packed in 3 polyethylene bags each 2.0 m Individually packed in 1 polyethylene bag each Holder for disposable gelatine filter units When the entered air flow rate cannot be attained, 375 Approx. 6.5 kg For gelatine membrane filters, max. 62 dB (A) in ±5% a temperature range of 15°–35°C 1–99 minutes, adjustable in 1-minute steps MD8 airscan 17655 (Gelatine disc filters) a corresponding new setting below this value. the display shows the max. attainable flow rate for ® air sampler ® air sampler + ®

3 242 air /h – 8 m 3 /h + ® 228 mm air sampler ® ® air sampler, 115 V, 60 Hz air sampler, 230 V, 50 Hz 3 /h adjustable in 100-liter steps medium for incubation and colony growth. can be placed directly on the agar culture After removing the sample, the gelatine filter e.g. within the scope of validation steps. user can calibrate the oped calibration unit (see accessories), the removal speed. By means of a specially devel- selectively and easily air flow rate and sample The MD8 airscan ® air sampler allows to adjust MD8 airscan

® locally,

Special brochures available on request. Order no. SM-1502-e and SM-4023-e Please refer to the following pages. Ordering information for consumables 69898525 17801 17803 Order number Accessories and replacement parts for the 16757 Order number Ordering information for the Charging time Battery charger output Battery charger input Battery Power supply Inclusive adapter Volume flow regulation Specifications for Specifications Dimensions (L Weight Noise level battery charge Operational life with one Fixed given sample volumes Volume flow adjustable – – – benefits AirPort MD8 offers the following care and for work safety. industry, for hospitals’ environmental biotechnology, the food and beverage the pharmaceutical industry, the AirPort MD8 is the air sampler for Battery-Powered Portable Air Sampler AirPort MD8 Ergonomic design and easy to clean. is guaranteed. constant performance during sampling Battery power level clearly indicated so universal use. Battery-powered and portable for + W + AirPort MD8 H)

AirPort MD8 Battery charger Holder for disposable gelatine filter units Adapter for BACTair™ on the AirPort MD8 air sampler and battery charger (69898525). AirPort MD8, complete with two adapters (17801 and 17803) Approx. 4.5 hours for empty battery 24 V/1000 mA Hz/600 V/47–63 mA 100–240 NiMH 16.8 Volt/3800 mAh 17803 (for BACTair™ Plates) 17801 (for disposable gelatine filter units) 300 Approx. 2.5 kg For gelatine membrane filters 48 dB (A) Approx. 4.5 hours for 50 l/min the sample volume can be chosen manually in 5-liter steps. 25, 50, 100, 250, 500, 750 and 1000 liters. In addition, 30 l/min., 40 l/min., 50 l/min. and 125 l/min. By an integrated impeller wheel.

135

+ AirPort MD8 165 mm or the impaction method with BACTair™. the gelatine membrane filter method measurement results AirPort MD8 uses For guaranteeing reliable and exact – – – –

The device can be calibrated locally. automatic shut-off. Parameters last used stored even after German and Spanish. of four languages; English, French, User-friendly prompting with the option volume flow and the sample volume. Flexible adjustment possibilities of the

Air Monitoring | AirPort MD8 Air Monitoring | Gelatine Membranes 8 as well as in smaller diameters. Special brochure available on request. Order no. SM-3011-e 12602--37----ALK 12602--47----ALK 12602--47----ALN 12602--50----ALK 12602--50----ALN 12602--80----ALK supplied with the the filter holder 17655 (80 mm diameter) are still available as filter discs, suitable for filter and a holder. Gelatine membrane filters units, each consisting of a gelatine membrane packed, es. Gelatine filter disposables are individually for collecting of airborne microbes and virus- air samplers (gelatine filter method) are used Gelatine filters in conjunction with the MD8 Order number Gelatine disc filter, sterile, sealed in units of five each in a polyethylene bag 17528--80----VPD 17528--80----BZD 17528--80----ACD Order number Disposable gelatine units, sterile, pack of 10 Sterilization Conditions for use Air flow rate Thermal resistance Gelatine filters Specifications of gelatine filters Specifications Gelatine Membrane Filters Filtration area Retention rates pre-sterilized and ready-to-connect MD8 airscan

® air samplers, 37 mm 47 mm 47 mm 50 mm 50 mm 80 mm Diameter but label on innermost bag Individually packed in 3 polyethylene bags each, Individually packed in 3 polyethylene bags each Individually packed in 1 polyethylene bag each Supplied pre-sterilized by gamma irradiation Room temperature, max. 30°C, max. air humidity 85% Approx. 2.7 l/min./cm Max. 60°C thickness approx. 250 µm Water soluble, pore size 3 µm, 80 mm diameter, 38.5 cm velocity and 80% rel. humidity. and 50% rel. air humidity. Phage T3, 99.94% at 0.3 m/s inlet 2. Coli phages: phage T1, 99.9% at 0.3 m/s inlet velocity inlet velocity. 1. Bac. subtilis niger spores 99.9995% at 0.25 m/s 2

– – – – benefits: air samplers offer the following features and Gelatine filters in conjunction with the MD8 prerequisite for virus sampling. The solubility of the gelatine filter is a by inhibitors. be measured. The sample is not affected media or low and high bacteria counts can can be cultivated in| soluble. Therefore, microbes in one sample Gelatine filters are completely water- and meaningful sampling time. collected microorganisms for a relevant The filter maintains the viability of T3 phages). for Bac. sub. niger spores, 99.94% for "Absolute” retention rate (99.9995% 2 at 50 50 100 50 100 50 Package size ∆P = 0.05 bar on different nutrient

High retention of particles Number of impaction holes Dimensions Material Specifications for Specifications 16757 Air Sampler Other BACTair™ Culture Media types on request. 14321-110----ACD 14320-110----ACD Order Number BACTair™ Culture Media Plates with Agar, 110 mm, individually, sterile packaged, 10 units Sterilization Special brochures are available on request. Order no. SM-4023-e and SL-2047-e 14301-110------K 1ZPX-D0002 17803 Accessories Microbiological Air Monitoring by the Impaction Method BACTair™ - Big Impact. past. sieves or slots have become a thing of the basis, are eliminated. Now, non-sterile sterilized for routine samplings on a regular tion heads with slots, which have to be plates. Metal sieve plates or metal collec- are integrated right into the culture media This means that the collection properties plates function directly as collection heads. onto culture media plates, where the airborne organisms that allows impaction A new developed system for sampling

BACTair™

400 holes, 116 Polystyrene Yeasts and molds Total Count Determination of Gamma irradiation > 0.65 µm AirPort MD8 BACTair™ individually, sterile packaged Covers for Adapter for +

24 mm

Plates, sterile, without media d 0.47 mm each BACTair™ Culture Media Plates, 10 BACTair™ on the AirPort MD8 air sampler Air Sampler for – Optimized geometry – Samples 1 m – Pre-filled with agar media – Integrated disposable sieve – Individually, sterile packaged BACTair™ offers the following benefits is ready-to-connect to the AirPort MD8. BACTair™ Culture Media Plates. BACTair™ sampler to draw the air stream over the This new method uses the AirPort MD8 air tion samplers. generally higher than that of other impac- exceptional sampling efficiency, which is and the 400 holes in the sieve plate yield The geometry of the culture medium plate BACTair™ incl. charger 3 in just 8 min Sabouraud Agar (acc. USP) Tryptic Soy Agar (TSA) Medium Type + 2 units

Air Monitoring | BACTair™ Air Monitoring | Accessories 10 Measuring battery for empty Charge time full battery life with Operational Connectors Dimensions Specifications for calibration unit * set and connection tube (PVC, 2 m). in which it is used), filter holder, connectors power supply unit (specific for the country supplied complete with battery charger| calibration device). The calibration unit is the actual air amount (actual value at the (desired value at the MD8) corresponds to is important that the shown air flow rate the scope of validation steps, for which it This is absolutely necessary above all within the calibration unit*. AirPort MD8 directly on the job by means of Order no. SL-2028-e Special brochure available on request. Weight temperature ambient Allowable protection Type of Max. error range The user can calibrate the MD8 airscan Calibration unit for the MD8 Air Accessories calibration of the MD8 at regular intervals Biotech technicians will carry out a contractual services, Sartorius Stedim can be signed. Within the scope of the Alternatively, a maintenance agreement

1–16 m Approx. 10 hours Approx. 4 hours Quick locks (bayonet principle) 200 mm max. (adjustable feet) Height, 182 mm min., Width, 390 mm with handles (without filter holder), Length, 300 mm Approx. 11 kg Min. 0°C, max. 40°C IP 40 1–16 m 3 3 /h, ±2% /h Samplers

® and

storage of a MD8 airscan A stable case for the transport and the Case off into another room. connector (exhaust), the waste air can be led etc. With this hose attached to the air outlet rooms, isolators, blow-fill-seal machines, has to be used in sterile rooms, operating the flexible plastic hose, if the autoclavable silicone hose is used instead of tion between filter and clamp 17037) are necessary for the connec- a holder (tripod 16970, double socket 16976, or 5 m), a connectors set and, if not available, distance from it, a flexible plastic hose (2 m airscan For the monitoring of isolators with Accessories for isolator application from each other. and unused filter holders are separated other side of the middle part, so that used the inserted filter holders are put on the middle part. After removing the sample, filter holders are put on one side of the conditions (LF cleanbench). The prepared then equipped with the filters under sterile first sterilized (by 180°C dry heat, 2 h), and filter holders and 2 end caps. The stack is It consists of a middle part, 10 numbered Aluminum stack clean-room classes is maintained). area (the barrier function between different air sampler remains outside the critical work construction makes it possible that the MD8 between the and outlet, for sterile air filtration inserted with PTFE membrane and sanitary flange inlet 17801---001 as well as a Sartofluor capsule holder for gelatine filter disposables 17659---003 (for tri clamp) and the filter flanges, connector 17659---001 or or 17030 (DN30), clamps 17033 for sanitary accessories such as adapters 17016 (DN25) placed directly at the If the disposable gelatine filter unit is not Tubing and connectors set ® , we recommend using stainless steel MD8 airscan MD8 airscan MD8 airscan ® ® , incl. accessories. and isolator. This MD8 airscan ® , but at a

® MD8

. The

17208 16756 Order numbers Accessories for the MD8 air samplers mended to use cellulose nitrate filters. humidity, high temperature), it is recom- If gelatine filters cannot be used (high Further consumables for air monitoring case. check-ups, a stack is recommended in this sterile conditions. For performing routine and then equipped with the filters under have to be sterilized by dry heat (180°C, 2h) are sterile-supplied, but the filter holders er supplied with the MD8 airscan 80 mm filter discs, suitable for the filter hold Gelatine membrane filters are still available as pack of 50 for use with stack Gelatine membrane filter, 80 mm, sterile, b) Via a PLC interface unit (1ZE---0003). with MD8 airscan a) Via a PC (with Microsoft 95/98 or higher) control configurations: from a distance, using either of two remote possibility of operating this air sampler connection to the MD8 airscan 17661 17660 17655 Order numbers Replacement parts for the stack 17656 Users of the MD8 airscan Accessories for remote control function

Case for MD8 airscan samplers Calibration unit for the MD8 air End cap Middle part 12602--80----ALK for gelatine filter type Individual filter holders samplers Aluminum stack for MD8 air ® dialog system and cable ® now have the ® (1ZE---0004). ® ® . The filters

1ZE---0004 1ZE---0003 Order numbers Accessories for remote control function 11301--80----ALN 13004--80----ALN 11404--80----ALN Order numbers Cellulose nitrate membrane filters, 80 mm diameter, 100 pieces/pack Further consumables for air monitoring 12602-080 ALK Order numbers Gelatine disc filters, 3 µm pore size, 80 mm, 50 pieces/pack Consumables used with stack 5185307TS-----SS 17801---001 17659---003 17659---001 17033 17030 17016 Order numbers Accessories for isolator application 17659 17658 17657 17662 17088 17085 Order numbers Tubing and connectors set

Remote control for MD8 airscan for PLC units Remote control (Interface) for MD8 airscan pre-sterilized in bags of 5 Cellulose nitrate membrane filters, 8 µm, white no grid, grid, pre-sterilized in bags of 5 Cellulose nitrate membrane filters, 0.8 µm, gray with white grid, pre-sterilized in bags of 5 Cellulose nitrate membrane filters, 0.8 µm, white with black polyethylene bag Gelatine disc filter, sterile, sealed in units of five each in a (dialog system software) Sartofluor Adapter for gelatine filter disposables, stainless steel stainless steel tri clamp, Connector (flexible hose to filter holder|adapter), hose nipple, stainless steel Connector (flexible hose to filter holder|adapter), 1/2" sanitary flanges, stainless steel Clamp for 1"–1 filter capsule, stainless steel connect MD8 airscan 1/2" Adapter sanitary (DN flange) 30 to hose barb to 1"–1 filter capsule, stainless steel inserted between the MD8 airscan sanitary flange inlet and outlet, for sterile air filtration connect MD8 airscan 1/2" Adapter sanitary (DN flange) 25 to hose barb to 1"–1 aluminum Connector (flexible hose to filter holder|adapter), Connector (air sampler inlet to flexible hose), aluminum Set of connectors (consisting of 17658 and 17659), aluminum Silicone tubing, sterilizable (1 m, state length required) Flexible PVC hose with reinforced ends (5 m) Flexible PVC hose with reinforced ends (2 m)

® MidiCap Capsule with PTFE membrane and ® ® to an isolator via flexible PVC hose and to an isolator via silicone tubing and a ® for use with PC ® and isolator ® designed

Air Monitoring | Accessories Colony Counting | Gridded Membranes for Colony Counting 12 lettering. Quality control par excellence! Each envelope is checked for readable perfect grid printing and is free of particles. with no slippage under the edge seal, has damaged in any way, is positioned correctly membrane is checked to ensure it is not machine with ultra-modern stamping. Each required the construction of a new packaging The increasing demand on these filters individual envelope. identification and lot number printed on each packs and conform with GLP, having filter contamination of remaining filters in opened vidually packed, they avoid the possibility of and save preparatory time. As they are indi- They are pre-sterilized and ready-to-use they offer. quality control because of the user benefits become standard for routine microbiological Sterile, individually packed filters have long to ISO Standards, Sterile and Individually Packaged, for Colony Counting Gridded Membrane Filters from

Cellulose Nitrate (Cellulose Ester) acc. Operation Procedures. Drug Administration and Internal Standard Hygiene (Journal of Hygiene), the US Federal Institute of Brewery), the Zentralblatt für Department of Agriculture, the VLB (German the United States Pharmacopoeia, the US the U.S. Environmental Protection Agency, the testing procedures for packaging stuff, Guideline), the National Canners Association, Commission, the MNO (Mineral|Table Water methods of the Central European Brewery in the journal of the Institute of Brewing, the nal of Food Protection, the method described Lafourcade, the method described in the jour- the method described by Lanaridris & Lafon- ISO Guidelines, the LMBG (German food law), the International Fruit Juice Producers, the Analysis, the International Dairy Federation, Commission for Uniform Methods of Sugar German Pharmacopoeia, the International 98/83, the European Pharmacopoeia, the nity, the European Drinking Water Guideline Standards), the European Brewery Commu- Micro DGHM (German Association of Hygiene and Water Guideline, the British Standards, the cial Ana Standard Methods, the Association of Offi- American Society for Microbiology, the APHA ards), the American Petroleum Institute, the accordance with the AFNOR (French Stand- Sartorius Stedim Biotech Nutrient Pads in tured for use especially at the same time with In addition to this they have been manufac- 7899-2, ISO 8199, ISO 9308-1 and ISO 16266. with the following norms: ISO 7704, ISO These membrane filters are in accordance biology), the DIN Guidelines (German lytical Chemists, the British Drinking

containing antibiotics (page 22). mainly in the sterility testing of solutions Hydrophobic edge membranes are used sterile packaged (page 20). acetate membrane filters, white, individually, Cellulose nitrate (cellulose ester) and (page 18). filters, gridded, non-sterile packaged Cellulose nitrate (cellulose ester) membrane Additional membrane filters and released according to ISO 7704. filter lot, these membranes are also tested Sartorius Stedim Biotech 0.45 µm membrane promotion on High Flow Membranes. As every puts. Especially E. coli shows best growth times due to higher flow rates and through- membrane filters allows shorter filtration pore structure of the new 0.45 µm HighFlow to traditional 0.45 µm membranes. The special provides 30% higher flow rates in comparison special variant is the High Flow membrane. It biological analysis is an 0.45 µm filter. One The standard membrane filter for micro High flow membranes best contrast to the colonies and particles. growth. The various filter colors allow the microcolonies, but does not influence the especially for higher bacteria counts and for 3.1 growth. The printed grid with a size of with high flow rates and optimum colony a material which assures effective retention All membranes are made of cellulose nitrate, The membrane filters Specifications + 3.1 mm makes the counting easier, cellulose

Colony Counting | Gridded Membranes for Colony Counting Colony Counting | Microsart® e.motion 14 1ZE---0028 a time – it’s simple, “fail-safe,” and fast. dispensing of several membrane filters at Specifications of the Microsart The controller specially developed for the in the process are now problems of the past. their packaging or that even get damaged Membranes that accidentally slide out of each filter is quickly and reliably dispensed. Thanks to their new motorized traction roller, can be optionally connected to the or at the touch of a button. A pedal switch in a touch-free mode via an optical sensor removed from their sterile package – either The membrane filters are automatically discs. for individually sterile cellulose nitrate filter Fully automated membrane Microsart Max. power Frequency Operating voltage Weight Dimensions (L Order number for Certificates Dispenser delay Dispensing speed 16712 ® e.motion prevents unwanted

® + e.motion Dispenser H

+ W) in mm Microsart Microsart filter ® e.motion ® dispenser e.motion Pedal (foot switch) for fully automated membrane filter 50–60 Hz 110 V/230 V optional 2.9 kg 204 EN 50082-1 and -2, EN 61010 European Standards EN 50081-1 and –2, CE Mark and EMC Directive, 5 sec 0.5 sec Consumption 10 W Microsart dispenser. + 213 dispenser

® +

e.motion dispenser 165 – Easy-to-clean – Easy insertion of the filter band – – Compact design – Works by touch button – Works hands-free by an optical sensor – dispenser: from when using the Some of the advantages you will benefit particle testing and microscopy Membrane filters for colony counting, Applications you need in your lab. ideal, giving you the versatility and flexibility transport. Both its functions and design are dispenser’s low weight makes it easy to be connected to control the available so that other sensor systems can Microsart allows quick and easy cleaning. The The clear, compact design of the sprocket feed roll technology Rapid and reliable transport due to dispenser Fully automated membrane filter dispenser, Microsart ® e.motion has an interface port ® e.motion dispenser. Microsart dispenser dispenser. The ® e.motion dispenser

White| sterile packaged, without protective paper Diameter 47 mm or 50 mm, in pack of from when using the Microsart Some of the advantages you will benefit e.motion – reliable help in your lab. – Gamma irradiated, 25kGray – High Flow membranes available – Product data are printed on – Packaged on a special pleated band – Protective paper-free – Multi-fit: Fits into various dispensers – 0.45 µm are acc. to ISO 7704 – membrane filters: open and seal for storage. Microsart band, and is designed so that it is easy to filters individually sealed on a special pleated quantity. Each box contains 100 membrane to completely use up a complete package rapidly as needed, even without having niently inserted, and changed easily and Order numbers for for the Microsart The membrane filter band specially designed Microsart White| White| White| White| White| White| White| White| White| White| White| White| Green| Green| Gray*| Gray*| Gray*| Gray*| Gray*| Gray*| Gray*| Microsart * Gray membranes after wetting black microorganisms Outstanding recovery rates for white white white white white white white dark green black dark green black black black black black black black black black green green green ® e.motion Membrane Filters are also available together with Nutrient Pads (page 25).

® e.motion Membrane Filters ® e.motion can be conve Microsart ® ® e.motion Membrane Filters e.motion

11407Z-47----SCM 11407Z-50----SCM 114H6Z-47----SCM 114H6Z-50----SCM 11406Z-47----SCM 11406Z-50----SCM 11404Z-47----SCM 11403Z-47----SCM 11403Z-50----SCM 11402Z-47----SCM 139H6Z-47----SCM 13906Z-47----SCM 13906Z-50----SCM 13806Z-47----SCM 13806Z-50----SCM 130H6Z-50----SCM 13006Z-47----SCM 13006Z-50----SCM 13005Z-47----SCM 13005Z-50----SCM 13004Z-47----SCM 13004Z-50----SCM ®

3 +

100 membranes, individually, sterile packaged nitrate (cellulose ester), gridded, individually, Please refer to the membrane type: Cellulose Specifications

0.2 µm 0.2 µm 0.45 µm High Flow 0.45 µm High Flow 0.45 µm 0.45 µm 0.8 µm 1.2 µm 1.2 µm 3 µm 0.45 µm High Flow 0.45 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm High Flow 0.45 µm 0.45 µm 0.65 µm 0.65 µm 0.8 µm 0.8 µm 15

Colony Counting | Microsart® e.motion Colony Counting | Gridded Membranes 16 Chemical compatibility Thickness acc. to DIN 53105 Thermal resistance Sterility test acc. to ISO 7704 Growth Promotion Test Design Specifications – Gamma irradiated, 25kGray – Certified quality – – High Flow membranes available – 0.45 µm are acc. to ISO 7704 – filter: from when using this type of membrane Some of the advantages you will benefit particle testing and microscopy Membrane filters for colony counting, Applications Gridded, Individually, Sterile Packaged Cellulose Nitrate (Cellulose Ester) Membrane Filters, **) *) Coliform retention 1 Pore size Typical performance rates for various pore sizes Recovery rate lot-released acc.

to ISO 7704 Flow rate for water per cm Three different colors available micro Outstanding recovery rates for bar acc. to DIN 58355 Water and Waste Water Pore size determined by quantitative retention of Serratia marcescens in accordance with the Standard Methods of filters utilized for liquid filtration. the ASTM Document F 838-83 (1993) Standard test method for determining bacterial retention of membrane Pore size determined by quantitative retention of Brevundimonas diminuta in accordance with organisms

2 at

in ml/min Nitrate type 113 (page in section “Chemical Compatibility” under Cellulose several other organic solvents. Detailed information Aqueous solutions (pH 4-8), hydrocarbons and 115–145 µm 130°C max. Sterile – – – No enhancement or inhibition by the grid lines and gridded 47 or 50 mm in diameter, white, grey or green in %

in %

process No enhancement or inhibition by the sterilization extractables No enhancement or inhibition due to chemical

20 100 0.2 µm* > 90

68). 70 100 0.45 µm** > 90

100 100 0.45 µm > 90 High Flow**

130 n. a. 0.65 µm > 90

Pore size particle count & microscopy, White membrane with black grid, for detection of bacteria with dyed media, 0.2 µm 0.45 µm 0.45 µm High Flow* 0.65 µm 0.8 µm 1.2 µm 0.45 µm particle count and microscopy, White membrane with green grid, for detection of bacteria with dyed media, 0.45 µm High Flow* 0.65 µm 1.2 µm 0.45 µm sterile packaged to light-colored or transparent bacteria colonies, Green membrane with dark-green grid, providing optimal contrast

0.45 µm sterile packaged of yeasts and molds, particle count and microscopy, Gray membrane (after wetting, black) with white grid, for detection 0.65 µm 0.8 µm

Order No. 11407--50----ACR 11407--50----ACN 11407--47----ACR 11407--47----ACN 11406--50----ACR 11406--50----ACN 11406--47----ACR 11406--47----ACN 114H6--50----ACR 114H6--50----ACN 114H6--47----ACR 114H6--47----ACN 11405--50----ACN 11405--47----ACN 11404--50----ACN 11404--47----ACR 11404--47----ACN 11403--50----ACR 11403--50----ACN 11403--47----ACR 11403--47----ACN 13906--50----ACR 13906--50----ACN 13906--47----ACR 13906--47----ACN 139H6--50----ACN 139H6--47----ACR 139H6--47----ACN 13905--47----ACN 13903--47----ACN 13806--47----ACN 13806--47----ACR 13806--50----ACN 13806--50----ACR 13006--50----ACR 13006--50----ACN 13006--47----ACR 13006--47----ACN 13005--50----ACR 13005--50----ACN 13005--47----ACN 13004--50----ACN 13004--47----ACR 13004--47----ACN type 114, individually, sterile packaged

type 139, individually, sterile packaged

Diameter 50 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 47 mm 50 mm 47 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 47 mm 50 mm 47 mm 47 mm 47 mm 47 mm 47 mm 47 mm 50 mm 50 mm 50 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 50 mm 47 mm 47 mm type 138, individually, type 130, individually,

Pack size 1,000 100 1,000 100 1,000 100 1,000 100 1,000 100 1,000 100 100 100 100 1,000 100 1,000 100 1,000 100 1,000 100 1,000 100 100 1,000 100 100 100 100 1,000 100 1,000 1,000 100 1,000 100 1,000 100 100 100 1,000 100

Colony Counting | Gridded Membranes Colony Counting | Gridded Membranes 18 Growth Promotion Test Design Specifications – Three different colors available – 0.45 µm are acc. to ISO 7704 – filter: from when using this type of membrane Some of the advantages you will benefit particle testing and microscopy Membrane filters for colony counting, Applications Gridded, Non-Sterile Packaged Cellulose Nitrate (Cellulose Ester) Membrane Filters, **) *) Coliform retention 1 bar acc. to DIN 58355 Chemical compatibility Thickness acc. to DIN 53105 Thermal resistance acc. to ISO 7704 Recovery rate lot-released acc. Pore size Typical performance rates for various pore sizes to ISO 7704 Flow rate for water per cm microorganisms Outstanding recovery rates for with the Standard Methods of Water and Waste Water Pore size determined by quantitative retention of Serratia marcescens in accordance filters utilized for liquid filtration. ASTM Document F 838-83 (1993) Standard test method for determining bacterial retention of membrane Pore size determined by quantitative retention of Brevundimonas diminuta in accordance with the

2 at

in ml/min – No enhancement or inhibition by the grid lines grey or green and gridded 25, 47 or 50 mm in diameter, white, in % Nitrate type 113 (page in section “Chemical Compatibility” under Cellulose several other organic solvents. Detailed information Aqueous solutions (pH 4-8), hydrocarbons and 115–145 µm 130°C max. – in %

extractables No enhancement or inhibition due to chemical

20 100 > 90 0.2 µm*

68). 70 100 > 90 0.45 µm**

130 n. a. > 90 0.65 µm

Pore size particle count & microscopy, White membrane with black grid, for detection of bacteria with dyed media, 0.2 µm 0.45 µm 0.65 µm 0.8 µm 1.2 µm 0.45 µm particle count and microscopy, White membrane with green grid, for detection of bacteria with dyed media, 0.45 µm colored or transparent bacteria colonies, Green membrane with dark-green grid, providing optimal contrast to light- 0.45 µm and molds, particle count and microscopy, Gray membrane (after wetting, black) with white grid, for detection of yeasts 0.65 µm 0.8 µm

Order No. 11407--50------N 11407--47------R 11407--47------N 11407--25------N 11406--50------R 11406--50------N 11406--47------R 11406--47------N 11406--25------N 11405--47------N 11404--50------N 11404--47------N 11404--25------N 11403--50------N 11403--47------N 11403--25------N 13906--50------R 13906--50------N 13906--47------R 13906--47------N 13806--50------R 13806--50------N 13806--47------R 13806--47------N 13006--50------N 13006--47------R 13006--47------N 13006--25------N 13005--50------N 13005--47------N 13004--50------N 13004--47------N type 114, non-sterile

type 139, non-sterile

type 138, non-sterile type 130, non-sterile Diameter 50 mm 47 mm 47 mm 25 mm 50 mm 50 mm 47 mm 47 mm 25 mm 47 mm 50 mm 47 mm 25 mm 50 mm 47 mm 25 mm 50 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 47 mm 50 mm 47 mm 47 mm 25 mm 50 mm 47 mm 50 mm 47 mm

Pack size 100 1,000 100 100 1,000 100 1,000 100 100 100 100 100 100 100 100 100 1,000 100 1,000 100 1,000 100 1,000 100 100 1,000 100 100 100 100 100 100

Colony Counting | Gridded Membranes Colony Counting | Membranes without Grid 20 adsorption characteristics. rates and thermal stability with very low acetate, a material which combines high flow rates and optimum colony growth or cellulose assures effective retention with high flow nitrate (cellulose ester), a material which The membranes are made of even cellulose Materials individual envelope. identification and lot number printed on each packs and conform with GLP, having filter of contaminating remaining filters in opened individually packed, they avoid the possibility to-use and save preparatory time. As they are they offer. They are pre-sterilized and ready- quality control because of the user benefits become standard for routine microbiological Sterile, individually packed filters have long Filters, White, Individually, Sterile Packaged Cellulose Nitrate (Cellulose Ester) and

– Gamma-irradiated, 25kGray – Certified quality – 0.2 µm are validated by BCT – 0.45 µm are acc. to ISO 7704 – Defined particle retention – filter: from when using this type of membrane Some of the advantages you will benefit microscopy sterility testing, particle testing and Membrane filters for colony counting, Applications by the Bacteria Challenge Test. buffer and sera. This membrane is validated sterile filtration, such as nutrient media, pore size of 0.2 µm is the filter of choice for 11107, a white CA membrane filter with a filter (e. g. 0.45 µm). underlying bacteria-retentive membrane microbes are trapped on the surface of the microorganisms to pass through. These suspended particles, whereas it allows bacteriological analyses. It retains the coarse in a special prefilter attachment (16807) for a pore size of 8 µm is used as a prefilter 11301, a white CN membrane filter with Additional applications microorganisms Outstanding recovery rates for Cellulose Acetate Membrane

Specifications Thickness acc. to DIN 53105 Thermal resistance Sterility test acc. to ISO 7704 Growth Promotion Test Design Pore size particle count & microscopy, Cellulose nitrate membrane filters, white, for colony counting, sterility testing, Chemical compatibility 0.45 µm 0.65 µm 0.8 µm 1.2 µm 3 µm 8 µm 0.2 µm ing, particle count & microscopy, Cellulose acetate* membrane filters, white, for colony counting, sterility test- 0.45 µm * If cellulose nitrate is not compatible

Order No. 11306--50----ACN 11306--47----ACN 11306--25----ACN 11305--50----ACN 11305--47----ACN 11304--50----ACN 11304--47----ACN 11303--50----ACN 11303--47----ACN 11302--50----ACN 11302--47----ACN 11301--50----ACN 11301--47----ACN 11107--50----ACN 11107--47----ACN 11106--50----ACN 11106--47----ACN

type 113, individually, sterile packaged

CN: 11 CN: 130°C max. | CA: 180°C max. Sterile – – No enhancement or inhibition by the 25, 47 or 50 mm in diameter, white type 111 (page Cellulose Nitrate type 113 and Cellulose Acetate tion in section “Chemical Compatibility” under several other organic solvents. Detailed informa- Aqueous solutions (pH 4–8), hydrocarbons and

to chemical extractables No enhancement or inhibition due sterilization process type 111, individually, sterile packaged 5– Diameter 50 mm 47 mm 25 mm 50 mm 47 mm 50 mm 47 mm 50 mm 47 mm 50 mm 47 mm 50 mm 47 mm 50 mm 47 mm 50 mm 47 mm 145 µm | CA: 120 µm (average value) 68).

Pack size 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100

Colony Counting | Membranes without Grid Colony Counting | Hydrophobic Edged Membranes 22 – – – different materials: The membranes are available in three Materials growth during incubation. out and the substance could inhibit microbial clamp zone wherefrom it could not be rinsed inhibitory substance into the membrane edge avoids the penetration of any growth- antibiotic characteristics. The hydrophobic ing of solutions containing substances with mainly for colony counting and sterility test- Hydrophobic edge membranes are used Individually, Sterile Packaged & Non-Sterile Cellulose Acetate and Regenerated Cellulose Membrane Filters, Hydrophobic Edged Growth Promotion Test Design Specifications acc. to ISO 7704 Chemical compatibility Thickness acc. to DIN 53105 Thermal resistance Sterility test characteristics. thermal stability with very low adsorption combines excellent chemical resistance and Regenerated cellulose, a material which with very low adsorption characteristics bines high flow rates and thermal stability Cellulose acetate, a material which com- flow rates and optimum colony growth which assures effective retention with high Cellulose nitrate (cellulose ester), a material

Cellulose Nitrate (Cellulose Ester), – No enhancement or inhibition by the grid lines white with black grid 25, 47 or 50 mm in diameter, white or – Acetate type 111 and Regenerated Cellulose type 184. under Cellulose Nitrate type 113, page Detailed information in section “Chemical Compatibility” all solvents and is compatible in a pH-range of 3-12. several other organic solvents, RC is resistant to almost Aqueous solutions (pH 4–8), hydrocarbons and RC: 160–200 µm CN: 115 CN: 130°C max. | CA and RC: 180°C max. Sterile –

extractables No enhancement or inhibition due to chemical process No enhancement or inhibition by the sterilization

–145 µm | CA: 120 µm (average value) | – Certified quality – 0.2 µm are validated by BCT – 0.45 µm are acc. to ISO 7704 – filter: from when using this type of membrane Some of the advantages you will benefit and sterility testing Membrane filters for colony counting Applications microorganisms Outstanding retention rates for

68, Cellulose

Pore size individually, sterile packaged 3 mm hydrophobic edge, for colony counting & sterility testing, Cellulose nitrate membrane filters, white with black grid, 0.2 µm 0.45 µm 0.45 µm individually, sterile packaged 6 mm hydrophobic edge, for colony Cellulose nitrate membrane filters, white with black grid, 8 µm for colony Cellulose nitrate membrane filters, white, 3 mm hydrophobic edge, 8 µm 0.45 µm 0.2 µm edge, for colony Cellulose nitrate membrane filters, white with black grid, 3 mm hydrophobic 0.2 µm individually, sterile packaged 3 mm hydrophobic edge, for colony Cellulose acetate* membrane filters, white with black grid, 0.45 µm 0.2 µm edge, for colony Cellulose nitrate membrane filters, white with black grid, 6 mm hydrophobic 0.45 µm 0.45 µm sterile, packaged of 10 discs per sleeve 3 mm hydrophobic edge, for colony Cellulose acetate* membrane filters, white with black grid, * If cellulose nitrate is not compatible 0.45 µm individually, sterile packaged for colony Regenerated cellulose* membrane filters, white, hydrophobic edged, 0.45 µm edge, for colony Cellulose acetate* membrane filters, white with black grid, 6 mm hydrophobic 0.45 µm 0.2 µm edge, for colony Cellulose acetate* membrane filters, white with black grid, 3 mm hydrophobic

counting & sterility testing, counting & sterility testing, type 184, 100 membranes per box, 18406--47----HDN 18406--47----ACN counting & sterility testing, counting & sterility testing, counting & sterility testing, counting & sterility testing, Order No. 13107--50----ACN 13107--47----ACN 13106--50----ACN 13106--47----ACN 13106--47----HEN 13101--50----AHN 13101--50------N 13101--47------N 13106--50------N 13106--47------N 13106--25------N 13107--50------N 13107--47------N 13107--25------N 13507--47----ACN 13106--47----HCN 13107--47----HCN 13506--50----ACN 13506--47----ACN 13506--47----ALS 13506--47----HCN 13506--47------N 13507--47------N

47 mm 47 mm counting & sterility testing, counting & sterility testing, counting & sterility testing,

type 131, non-sterile

type 131, non-sterile type 131, non-sterile type 135, non-sterile type 135, non-sterile Diameter 50 mm 47 mm 50 mm 47 mm 47 mm 50 mm 50 mm 47 mm 50 mm 47 mm 25 mm 50 mm 47 mm 25 mm 47 mm 47 mm 47 mm 50 mm 47 mm 47 mm 4 mm hydropho. edge 3 mm hydropho. edge 47 mm 47 mm 47 mm

type 131, type 131, type 135, type 135, Pack size 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100

Colony Counting | Hydrophobic Edged Membranes Colony Counting | Nutrient Pad Sets 24 or 50 mm diameters. microbial detection are available with 47 mm tailored to meet the special requirements of at the touch of a button. All membrane filters in a touch-free mode via an optical sensor or removed from their sterile package – either The membrane filters then are automatically Dispenser and can be conveniently inserted. specially designed for the Microsart Microsart also the appropriate membrane filters, which are All Nutrient Pad Set types are supplied with Inclusive membranes ICUMSA, etc. the food industry, such as LMBG, NCA and MEBAC or EBC, and recommendations of regulations, brewery guidelines, such as ards for Water and Foods, mineral water DIN and ISO standards, the American Stand- mendations: international pharmacopoeias, other interna drinking water directive, they comply with biological analysis. Aside from the European types to meet the diverse objectives of micro- more than 30 different Nutrient Pad Set Currently, Sartorius Stedim Biotech offers Compliance with International Standards reproducible results up to 24 months. assures such consistently high quality and to-use Nutrient Pads Sets unique: No other ready- This makes the Sartorius Stedim Biotech their entire shelf life up to 24 the high quality of our and temperature. As a result, it guarantees storage from fluctuations in humidity of the nutrient pads during transport and protects the sensitive formula constituents aluminum bag. This special packaging in bags each of these petri dishes are sealed in an inserted in a petri dish and sterilized. Ten nutrient pads, each of which is individually The standard Ready-to-use up to 24 months immediately. (or distilled) water they are ready to use 3.0–3.5 ml of sterile and demineralized media. Once they are moistened with Nutrient pads are sterile, dehydrated many microbiological testing procedures. to handle, they reduce labor and simplify filter method for 30 years. Practical and easy have been used successfully in the membrane Sartorius Stedim Biotech Nutrient Pad Sets Microbio with Nutrient Pad Sets – Dehydrated pre-sterilized and individually packaged. culture media around the globe Matching Membrane Filters for Economical, Time-saving ® e.motion Membrane Filters are logical Quality Control tional regulations and recom- NPS box contains 100 sterile NPS throughout months. ® e.motion culture

Media Pads in Petri Dishes, 24 months. temperature in a warehouse, up to Nutrient Pad Sets can be stored at room Trouble-free storage consistently quality and reproducible results. agar medium, in order to guarantee batch is compared with the corresponding During the production, each nutrient pad set Consistently quality microbiological equipment. laboratories without comprehensive Nutrient Pad Sets can also be used in Easy handling tion, cleaning, etc.). preparation of the nutrient media (steriliza- No time-consuming and labor-intensive Economy Benefits for the user

E. coli and coliforms Pseudomonas aeruginosa Enterococci Determination of filters (order no. -RDN = Microsart petri dishes, 100 per box, with 100 individually, sterile packaged 47 mm membrane Nutrient Pad Sets for total colony counting, Order numbers for nutrient pad sets in petri dishes Total count Total count E. coli and coliforms E. coli E. coli and coliforms Staphylococci, Staph. aureus Pseudomonas aeruginosa Enterococci Total count Total count E. coli and coliforms E. coli and coliforms E. coli and coliforms E. coli and coliforms Enterobacteria, E. coli 47 mm membrane filters (order no. -RDN = Microsart sterile packaged in petri dishes, 100 per box, with 100 individually, sterile packaged Nutrient Pad Sets for non-faecal, pathogenic bacteria, individually, Salmonellae Total count Total count Total count Total count E. coli and coliforms 47 mm membrane filters (order no. -RDN = Microsart sterile packaged in petri dishes, 100 per box, with 100 individually, sterile packaged Nutrient Pad Sets for E. coli, coliforms and enterobacteria, Total count Total count E. coli and coliforms E. coli and coliforms membrane filters (order no. -RDN = Microsart in petri dishes, 100 per box, with 100 individually, sterile packaged 47 mm Nutrient Pad Sets for other faecal bacteria,

Chromocult (7) Cetrimide (2) Azide NPS type (Filter type) R2A (1) Caso (1) Endo (9) Chromocult (7) ECD (2) Chapman (2) Cetrimide (2) Azide Standard TTC (1) R2A (1) Teepol m FC in closed petri dishes (2) m FC (2) MacConkey (2) Endo (9) Bismuth Sulfite (1) TGE Standard (1) Standard TTC I mod. (1) Standard TTC (1) Teepol| Yeast Extract (1) Extract (1) TGE Extract (1) Tergitol TTC (2) Tergitol TTC (2) ® | | e.motion Membranes) Tryptone Glucose Tryptone Glucose | | | KF Strep (1) KF Strep (1) Lauryl Sulphate (2) Lauryl Sulphate (2)

e.motion Membranes) individually, sterile packaged individually, sterile packaged in

® ® 1

e.motion Membranes) e.motion Membranes)

14063--47------N Order No. 14087--47------N 14087--47----RDN 14082--47------N 14074--47------N 14075--47----RDN 14075--47------N 14051--47----RDN 14084--47----RDN 14084--47------N 14053--47----RDN 14068--50----PDN 14068--47------N 14097--47------N 14053--47------N 14057--47------N 14051--47------N 14064--47------N 14085--47------N 14055--47------N 14055--47----RDN 14067--47----RDN 14076--47----RDN 14090--47------N 14076--47------N 14067--47------N 14056--47----RDN 14056--47------N individually, 2

Colony Counting | Nutrient Pad Sets Colony Counting | Nutrient Pad Sets 26 Thermophilic spore formers Determination of (order no. -RDN = Microsart 100 per box, with 100 individually, sterile packaged 47 mm membrane filters Nutrient Pad Sets for yeasts and molds, individually, Yeasts and molds Yeasts and molds Yeasts and molds Yeasts and molds Wild yeasts Acid-tolerant microorganisms Acid-tolerant microorganisms drink-spoiling microorganisms Lactobacilli and other soft other wine-spoiling organ. Lactobacilli and Pediococci Acid-tolerant microorganisms Acid-tolerant microorganisms Leuconostoc oenos and and mesophilic bacteria Yeasts and molds Mesophilic slime-forming bacteria microorganisms and other beer-spoiling Yeasts and molds Yeasts and molds yeasts and molds E. coli and coliforms, total count, with 100 individually, sterile packaged 47 mm membrane filters Nutrient Pad Sets starter kit, individually, sterile packaged in petri dishes, 100 per box, esp. Leu. mesenteroides Yeasts and molds Yeasts and molds Yeasts and molds Yeasts and molds Yeasts and molds (order no. -RDN = Microsart petri dishes, 100 per box, with 100 individually, sterile packaged 47 mm membrane filters Nutrient Pad Sets for product-spoiling microorganisms, individually, sterile packaged in Yeasts and molds Yeasts and molds and bacteria

® ® e.motion Membranes) e.motion Membranes)

Glucose Tryptone (2) NPS type (Filter type) Schaufus Pottinger| Sabouraud (10) Malt Extract (6) Malt Extract (8) Lysine (3) Orange Serum| Orange Serum MRS (1) Jus de Tomate| VLB-S7-S (2) Orange Serum| Orange Serum Schaufus Pottinger| m green yeast and mold (4) Weman (1) Schaufus Pottinger| m green yeast and mold (6) Schaufus Pottinger m green yeast and mold (5) Wort (1, 2, 3) Mixed types: Endo, Standard, Schaufus Pottinger| m green yeast and mold (6) Schaufus Pottinger| m green yeast and mold (8) Schaufus Pottinger m green yeast and mold (3) Wort (8) Wort (3) Wort (3) WL Nutrient (2) Wallerstein Nutrient| m green yeast and mold (8)

| | Tomato Juice (1) pH 5.5 (1) pH 3.2 (6) pH 5.5 (1) pH 3.2 (6) sterile packaged in petri dishes, | |

Order No. 14066--47------N 14069--47------N 14086--47------N 14086--47----CCN 14061--47------N 14070--47------N 14062--47----RDN 14077--47------N 14079--47------N 14062--47------N 14059--47------N 14096--47----RDN 14096--47------N 14072--47------N 14065--47------N 14080--47----RDN 14080--47------N 14095--47------N 14083--47------N 14091--47----RDN 14091--47------N 14058--47------N 14089--47------N 14092--47------N 14058--47----RDN

Other NPS types and NPS with Microsart 2) 1) Special brochure available on request f.o.c. Order no. SM-4017-e. Membrane Filters: Most of the NPS types are also available with but --47------N replaced by --50------N. Nutrient Pad Set with 50 mm membrane filter as above, Diameter of the membrane filter, 47 mm. Order number for ( (9) (8) (7) (6) (5) (4) (3) (2) (1) within brackets: corresponding nutrient media. The supplied membrane filter type is listed The membrane filters are selected for optimum growth, together with the 10)

= Gray (after wetting black) with white grid, 0.45 µm pore size, High Flow = White with green grid, 0.45 µm pore size, High Flow (ideal for E.coli) = Gray (after wetting black) with white grid, 0.45 µm pore size = White with black grid, 0.45 µm pore size = Gray (after wetting black) with white grid, 0.8 µm pore size = White with green grid, 1.2 µm pore size = White with green grid, 0.65 µm pore size = Gray (after wetting black) with white grid, 0.65 µm pore size = White with green grid, 0.45 µm pore size = Green with dark-green grid, 0.45 µm pore size Order number as above, but ---N replaced by -RDN. free of charge. Order no. SM-0001-e. On request, you can obtain this poster Pad Sets, described on the previous page. and typical applications for the Nutrient 70 cm The photo shows a poster, original size Nutrient Pad Set poster ® e.motion Membrane Filters on request. + 50 cm, with growth patterns Microsart ® e.motion

Colony Counting | Nutrient Pad Sets Colony Counting | Culture Media and Absorbent Pads 28 Determination of Agar Media in 250 ml bottles, 4 bottles per box They are available in tubes and in bottles. before a membrane filter is placed on it. incubation or for wetting an absorbent pad Liquid Liquid broth media cost-effective alternative for casting plates. petri dishes. Agar media in bottles are the sufficient for two 90 mm or three 60 mm agar plates. The content of one tube is available: Agar media in tubes are for pouring incubation. There are two different forms membrane filtration method or for direct isms is agar media. This can be used for the The traditional Agar Media Absorbent Pads and Petri Dishes Culture Media in Bottles and Tubes Total count Yeasts and molds Wild yeasts Lactobacilli and Pediococci and Determination of Agar Media in 20 ml tubes, 50 tubes per box other beer-spoiling organisms Total count Total count Yeasts and molds Acid-tolerant microorganisms Leuconostoc oenos and Concentration factor Lactose broth media, bottled concentrate, for drinking water analysis other wine-spoiling organ. Two times concentrated culture media broth for direct

culture media for microorgan-

Agar type – – pads are available in two diameters: manual dispensing device. The absorbent which fit onto the Sartorius Stedim Biotech come a membrane filter is placed on them. They appropriate liquid culture medium before absorbent pads are wetted with the Sartorius Stedim Biotech 1.4 mm thick Absorbent pads Nutrient Wort Lysine VLB-S7-S Agar type Nutrient Standard Wort Orange serum Jus de tomate Packaging (tomato juice) 4 bottles à 100 ml capacity. 50 mm with approx. 3.5 ml absorption capacity and 47 mm with approx. 3 ml absorption

pre-sterilized in plastic magazines,

Order No. 14144------A 14157------A 14143------A 14148------A Order No. 14137------K 14131------K 14138------K 14130------K 14140------K Order No. 14155------A

90 mm 60 mm Diameter Disposable petri dishes, auto-sterile, 100 per box (0.45 µm, green| dishes plus 100 membrane filters Absorbent Pad Set, 100 pads in petri petri dishes, sterile packaged Absorbent Pad Set, 100 pads in Description Absorbent Pads, 50 mm, sterile-packaged in petri dishes Absorbent Pads, 50 mm, sterile-packaged in (0.45 µm, white| in sleeves plus 100 membrane filters Absorbent Pad Set, 10 (0.2 µm, white| in sleeves plus 100 membrane filters Absorbent Pad Set, 10 Description Absorbent Pads, 47 mm, sterile packaged of 10 discs per sleeve (0.45 µm, white| plus 1,000 membrane filters Absorbent Pad Set, 10 Absorbent Pads, 10 Description Absorbent Pads, 10 Description Absorbent Pads, 47 mm, sterile packaged in other beer-spoiling organisms Lactobacilli and Pediococci and Determination of Broth media in 20 ml tubes, 50 tubes per box

black) black) green) dark green) + + 100 pads 100 pads + + + 10 pads 10 pads 100 pads

100 per box 100 per box Packaging incl. one 1,000 per box, 100 per box 100 per box Packaging incl. two dispensers 1,000 per box, incl. one 1,000 per box, Packaging Packaging VLB-S7-S Broth type

1 1 0 magazines, each with 0 magazines, each with dispenser dispenser

15400--50----FRN 14311--90------N 14311--60------N Order No. Order No. 15410--50----ALR 13706--47----ALN 13707--47----ALN Order No. 13906--47----APR 15410--47----ALR 15400--50------N Order No. Order No. 14127------K Order No.

1 1 00 pads 00 pads 29

Colony Counting | Culture Media and Absorbent Pads Colony Counting | Biosart® 30 – – – filtration procedures referenced in the: Media are in compliance with the membrane in liquid samples. Biosart for the analysis of microbial contamination accepted and the preferred method of choice The membrane filter method is worldwide Compliance with International Standards and sterilization required of reusable funnels. a vacuum manifold, eliminating the cleaning units designed to be placed onto the bases of Biosart ally sterilized, convenient plastic ampoules. for wetting the pad are available in individu- for membrane manipulation. Culture media Monitor into a petri dish eliminating the need remove the 100 ml funnel to convert the pad are ready to use. After filtration, just incorporated membrane filter and cellulose liquids. These sterile disposables with an cosmetics, food, beverages, water and other of microorganisms in pharmaceuticals, designed for the detection and enumeration Biosart Filtration area Filter diameter Pore size Capacity Pad Plug and adapter Membrane filter Housing Specifications Biosart units is optimal for these applications. beverages. The use of pharmaceuticals, water, cosmetics, foods and able technique for microbiological analysis of The membrane filtration method is the suit- Biosart Lot certificates Outlet Max. operating pressure 600/8-78-017. U.S. Environmental Protection Agency, Water and Waste Water, 20th edition Standard Methods for the Examination of Directive 98/83/EC on the quality of water) European drinking water directive (Council ® ® ® 100 Monitors are ready-to-use filter 100 Monitors have been specifically 100 Monitors ® 100 Monitors ready-to-use disposable ® 100 Monitors and 14.5 cm 47 mm 0.2 µm, 0.45 µm or 0.8 µm 100 ml, 10 ml graduations Cellulose Polyethylene membranes removable for filing Regenerated cellulose: white; green or grey, with grid; Cellulose nitrate (cellulose ester): choice of white, Polystyrene Recovery rate, sterility and specifications 6.5 Vacuum only

+ 1.5 mm 2

– Minimal amount of equipment needed – Ready to connect and easy to use Economical – Without any hydrophobic adhesive areas – Membranes available in various colors – Membranes meet ISO 7704 – Consistently recovery – Sterile or individually, sterile packaged Safe & reliable – High total throughput – High flow rate Superior performance from when using Biosart Some of the advantages you will benefit microscopy Colony counting, particle testing and Applications Flow Membranes. E. coli shows best growth promotion on High times due to 30% higher flow rates. special pore structure allows shorter filtration the new 0.45 µm High Flow membranes. The Biosart High Flow membranes – – – current editions of the USP and EP International Pharmacopoeia, such as the 1997 WHO Guidelines for Drinking Water Quality, DIN EN ISO 16266, ISO 8199 methods, such as ISO 7704, ISO 9308-1, International Standard's microbiological ® 100 Monitors are also available with

® 100 Monitors: Especially

Biosart Biosart Description available only in the U.S. and Canada 0.2 µm Pore size Biosart ** * 0.45 0.45 0.45 0.45 Biosart 0.8 µm 0.45 µm 0.45 µm 0.45 µm High Flow Biosart 0.45 µm 0.8 µm 0.45 µm 0.45 µm 0.45 µm 0.45 µm High Flow 0.2 µm Biosart polypropylene Biosart polypropylene silicone polypropylene and 0.45 µm Biosart silicone Lifter, ABS

Biosart 0.45 µm 0.45 µm Biosart Gray membranes after wetting black RC = Regenerated Cellulose CN = Cellulose Nitrate (Cellulose ester) μm μm High Flow μm μm High Flow ® ® ® ® ®

® ® ® ® ® 100 Membrane 100 Adapter, 100 Adapter, 100 Adapter, 100 Adapter, 100 Monitors, 100 ml, 47 mm, sterile, 48 units 100 Monitors, 100 ml, 47 mm, packaged on trays, sterile, 48 units 100 Monitor Adapters and Membrane Lifter 100 Monitors, 100 ml, 47 mm, sterile, 48 units, membrane fixed 100 Monitors, 100 ml, 47 mm, individually packaged, sterile, 48 units

black black dark green ® single base, 50 mm)

Order No. Order No. 16416 16415 16424 16403-47-06-VWMK 16403-47-H6-VWMK 16401-47-06-VWMK 16401-47-H6-VWMK 16403-47-04-V--K 16403-47-06-V--K 16401-47-06-V--K 16401-47-H6-V--K 16404-47-06----K 16403-47-06----K 16401-47-06----K 16401-47-H6----K 16401-47-07----K 16403-47-04----K 16402-47-06----K 16401-47-07--ACK 16414 16417 16401-47-06--ACK 16402-47-06--ACK 16403-47-06--ACK 31

Colony Counting | Biosart® Colony Counting | Biosart® 32 Acid-tolerant microorganisms processes Bacteria in fermentation Yeasts and molds and bacteria Yeasts and molds Yeasts and molds Yeasts and molds Yeasts and molds Pseudomonas aeruginosa Enterococci E. coli and coliforms E. coli and coliforms E. coli and coliforms Total count Total count Total count Total count E. coli and coliforms Biosart parameters have been passed successfully. promotion, sterility, physical and technical Stedim Biotech in-house tests of growth standards every batch has passed Sartorius procedure and the stringent quality control Within the scope of the quality assurance of the food and beverage industries. Foods, mineral water regulations, guidelines ards, the American Standards for Water and tional pharmacopoeias, DIN and ISO stand- regulations and recommendations: Interna- Nutrient Media comply with international which have a 9-month shelf life). Biosart for Endo, KF Strep, Lauryl Sulfate and Tergitol media have a shelf life of 12 month (except under proper conditions (+4°C), the culture with 2.5 ml and a lot certificate. If stored contains 50 ampoules with sterile media, each Determination of sterile-packaged in ampoules, 50 units ampoules. in use and eliminating the handling of glass Biosart Each box of Biosart Biosart ® ® 100 Nutrient Media are convenient 100 Nutrient Media, 2.5 ml, individually, ®

Nutrient Media

® 100 Nutrient Media

Orange Serum Wallerstein Differential WL Differential| Wallerstein Nutrient WL Nutrient| Wort selective m Green yeast and mold Schaufus Pottinger m Green yeast and mold Sabouraud (acc. USP) Cetrimide KF Strep| Tergitol TTC Lauryl Sulfate| m FC m Endo Total Count TTC TGE| R2A (acc. EP) Caso (acc. USP) Media type ® 100 Total Count

Azide

– Long shelf life – Ready-to-use Economical – Consistently recovery – – Certificate of Quality for every batch – Safe & reliable from when using Biosart Some of the advantages you will benefit Colony counting Application

standards In compliance with international Pre-sterilized media

Teepol

| 16400-02----OS-K 16400-02----WL-K 16400-02----WN-K 16400-02----WZ-K 16400-02----GS-K 16400-02----MG-K 16400-02----SB-K 16400-02----CE-K 16400-02----KF-K 16400-02----TT-K 16400-02----MF-K 16400-02----EN-K 16400-02----TZ-K 16400-02----TC-K 16400-02----RA-K 16400-02----CA-K Order No. 16400-02----LS-K ® 100 Media:

Microsart® @filter 100 | Microsart® @filter 250 Sterile disposable filter units for advanced colony counting

The process of producing pharmaceuticals Microsart® @vance® and bringing new drugs to the market is The Microsart® product family consists of all becoming an increasingly costly business. the most recent products from SSB for micro- The pharmaceutical and biotech industries biological analysis, which are especially char- are driven by the need to optimize their acterized by innovation and clever design. work flows and increase efficiency without The Microsart® @filter unit kicks off the new compromising their level of safety. Products product line Microsart® @vance®. @vance® and raw materials used in the pharmaceutical stands for even more progress and intelligent or biotech industry require control of micro- design, enhanced safety and thus more bial levels during processing and handling. reliable results. The products in the Microsart® Microorganisms in liquids are quantified by @vance® line have been specially developed the membrane filtration method. Use of this for analyses in the pharmaceutical and bio- membrane filtration method allows accurate technological industry. Following the trend of quantification of bacteria, yeasts and molds using single-use products, these products are when low counts in a high sample volume are delivered sterile, ready-to-use and can be dis- anticipated. All components of the filtration posed of in an environmentally friendly man- system must comply with international ner. Microsart® @filter not only saves time and guidelines, such as USP, EP or ISO standards. labor costs but minimizes the risk of secondary contamination – that's advanced colony Description counting by Sartorius Stedim Biotech. Microsart® @filter 100 and 250 filter units are a ready-to-use combination funnel, filter Microsart® Funnel Dispenser base and gridded membrane in one unit. The Funnel Dispenser for secure removal of The range of Microsart® @filter types has single, sterile Microsart® @filter has proven been tailored to meet individual needs: It is itself in practice. Even after opening the bag,

possible to choose between two volume sizes, the remaining funnels are protected from @filter 250 100 ml and 250 ml, different pore sizes and secondary contamination. The Microsart® ® different filter colors for contrasting back- Funnel Dispenser is made of high-grade grounds during evaluation. The filter units stainless steel, the dispenser opening is made exist as tray versions with lids or are stacked of polypropylene and contains a silicone in bags for safe removal using the Microsart® O-ring. All these materials guarantee reliable Funnel Dispenser. autoclaving.

® Despite the diversity of Microsart @filters Applications @filter 100 | Microsart ® one thing is common: The optimal design. Colony counting and microscopy – Click-Fit fastening allows for easy removal of funnels Some of the advantages you will benefit from – Leaking-free procedure due to innovative when using Microsart® @filter units: Click-Fit and bayonet closures – Bayonet closure allows for easy mounting Safe and reliable and removal of units – Sterile packaged – Sterile Filter Base with recesses allows Sterilization at the point of use is for simple membrane removal not required – Innovative geometry of the funnel allows – Fully disposable base and funnel Colony Counting | Microsart for effective rinsing after filtration Preparation-and sterilization-free (no sample residue is left in the funnel) procedure reduces the risk of secondary contamination They have been specifically developed for the – Optimized design and materials detection and enumeration of microorgan- No liquid remains after filtration, isms in pharmaceuticals, biopharmaceuticals eliminates the need of rinsing and cosmetics. Easy handling – Click-Fit closure Fast in routine analysis, eliminates the risk of leakage

Economy – Adaptable on Combisart® Given flexibility, no additional investment required – Transparent funnel material Visibility of the complete filtration

33 Specifications

Specifications Materials Funnel: Polypropylene Base: Polypropylene Membrane filter: Cellulose Nitrate (C. Ester), Regenerated Cellulose; choice of various colors and grids Capacity 100 ml, graduations at 20, 50 and 100 ml 250 ml, 50, 100, 200 and 250 ml graduations Filter diameter 47 mm, prefilter 40 mm (particle testing only) Filtration area 13.2 cm2 Max. operating pressure Vacuum only Sterilization Ethylene oxide Lot certificate Recovery rate, sterility and performance test

Microsart® @filter 100, sterile disposable filter units with lid, 47 mm, 100 ml, packaged on trays, ideal for the use in clean benches, 24 units Pore size Membrane filter* color | grid color Order No. 0.2 CN white | black 16D01--10-07--TG 0.45, High Flow CN white | black 16D01--10-H6--TG 0.45, High Flow CN gray | white** 16D03--10-H6--TG 0.45 CN green | dark green 16D02--10-06--TG

@filter 250 0.45 RC white (w/o grid) 16D05--10-06--TG ®

Microsart® @filter 250, sterile disposable filter units with lid, 47 mm, 250 ml, packaged on trays, ideal for the use in clean benches, 16 units Pore size Membrane filter* color | grid color Order No. 0.2 CN white | black 16D01--25-07--TF 0.45, High Flow CN white | black 16D01--25-H6--TF @filter 100 | Microsart ® 0.45, High Flow CN gray | white** 16D03--25-H6--TF 0.45 CN green | dark green 16D02--25-06--TF 0.65 CN gray | white** 16D03--25-05--TF

Microsart® @filter 100, sterile disposable filter units, 47 mm, 100 ml, stacked and packaged in bags, ideal for the use with Microsart® Funnel Dispenser, 60 units Pore size Membrane filter* color | grid color Order No. 0.2 CN white | black 16D01--10-07--BL Colony Counting | Microsart 0.45, High Flow CN white | black 16D01--10-H6--BL 0.45, High Flow CN gray | white** 16D03--10-H6--BL 0.45 CN green | dark green 16D02--10-06--BL 0.45 RC white (w/o grid) 16D05--10-06--BL

Microsart® @filter 250, sterile disposable filter units, 47 mm, 250 ml, stacked and packaged in bags, ideal for the use with Microsart® Funnel Dispenser, 48 units Pore size Membrane filter* color | grid color Order No. 0.2 CN white | black 16D01--25-07--BK 0.45, High Flow CN white | black 16D01--25-H6--BK 0.45, High Flow CN gray | white** 16D03--25-H6--BK 0.45 CN green | dark green 16D02--25-06--BK 0.65 CN gray | white** 16D03--25-05--BK

Accessories Description Order No. Microsart® Funnel Dispenser 16A08 Funnel dispenser for secure removal of single, sterile Microsart® @filter packaged in bags

34 * CN = Cellulose Nitrate (Cellulose ester), RC = Regenerated Cellulose ** Gray membranes after wetting black Sterile disposable Microsart No liquid is retained in the filter funnel. of the system sub The optimized shape allows thorough rinsing inner diameter ensures a high flow rate. to allow accurate sample volumes. The large a Click-Fit closure. Graduations are is elastic enough for optimal sealing with The funnel is made of polypropylene and thus practically fitted on. The sample is filtered. support. A Microsart membrane is placed on a stainless steel filter A Sartorius Stedim Biotech 47 mm gridded products. beverages, pharmaceutical and cosmetic in the routine testing of water, food and performance of the filtration steps required various sample volumes. They allow quick which are available for the filtration of Microsart Description reliability and time saving. filter funnel is the ideal combination for too laborious for routine use. A disposable can be sterilized by autoclaving, but this is properly performed. Alternatively, the funnels methods can be insufficiently reliable if not flaming or with hot water. Both of these are usually sanitized between samples by rials which are used for membrane filtration funnels made of stainless steel or other mate- a necessary basic requirement. The the equipment used for processing samples is In microbiological quality control, sterility of Microsart ®

Funnels are sterile ® ® Funnel 250 Funnel 100 sequent to filtration. ® Funnel is simply and funnels with Click-fit plastic funnels, re-useable marked

from when using Microsart Some of the advantages you will benefit microscopy Colony counting, particle testing and Applications autoclaving. O-ring. All these materials guarantee reliable of polypropylene and contains a silicone stainless steel, the dispenser opening is made Funnel Dispenser is made of high-grade Microsart secondary contamination. The Microsart the remaining funnels are protected from itself in practice. Even after opening the bag, – – – addition to existing single, sterile Microsart The Funnel Dispenser for secure removal of The Microsart Microsart after filtration. that the membrane can be removed easily are eliminated. Lateral notches make sure the counting of the colony growth difficult, Thus wrinkled membranes, which make plane positioning of the membrane filter. The slightly recessed frit ensures the Microsart in routine testing. particularly useful when using manifolds see when filtration has been completed, No more holding hot funnels! And, you can Simpler handling spending time sanitizing it! Just change the funnel, rather than Time-saving certain prevention of cross contamination! Use a new, sterile funnel for each test for Reliable results ® ® ® Combi.jet stainless steel manifolds. Funnel Dispenser Base 47 mm ® Base 47 mm is the perfect Combisart ® Funnels has proven ® Funnel 100: ®

and

Colony Counting | Microsart® Funnel 100 | Microsart® Funnel 250 Colony Counting | Microsart® Funnel 100 | Microsart® Funnel 250 36 will find on the following pages. Silicone O-ring for Microsart by bayonet closure Replacement frit for Microsart Microsart Optimized for the use with 47 mm membranes, Click-Fit closure for Microsart Description Description Combisart sterile Microsart Funnel dispenser for secure removal of single, Further information about Microsart Description Accessories and replacement parts Microsart Microsart Microsart Filtration area Filter diameter Capacity Material Specifications Specifications Microsart Lot certificate Sterilization Max. operating pressure

® ® ® ® ® ® Funnel, Microsart Base 47 mm, with frit, stainless steel base for Funnel Dispenser Funnel 250, sterile in 6 sealed bags Funnel 100, sterile in 5 sealed bags ® ® and Microsart Funnel 250, sterile disposable funnels, 250 ml, 96 units Funnel 100, sterile disposable funnel, 100 ml, 100 units

® Funnels ® Combi.jet Microsart ® ® @filter and other funnel types sealed Base 47 mm male thread (pack size 3) ® Base, stainless steel 13.2 cm 47 mm, prefilter 40 mm (particle testing only) 250 ml, 100 ml, graduations at 20, 50 and 100 ml Polypropylene Sterility and performance test Ethylene oxide Vacuum only ®

Combi.jet and 2 graduations at 50, 100, 200 and 250 ml

Combisart

® stainless steel manifolds you

Order No. Order No. Order No. 1ZU---0001 6980274 1ZU---0002 16A07--25------N 16A08 16A07--10------N Biosart® 250 Funnels

In microbiological quality control, sterility of Applications the equipment used for processing samples is Colony counting, particle testing and a necessary basic requirement. The reuseable microscopy funnels made of stainless steel or other materials which are used for membrane filtration Some of the advantages you will benefit are usually sanitized between samples by from when using Biosart® 250 Funnels: flaming or with hot water. Both of these methods can be insufficiently reliable when Superior performance not properly performed. Alternatively, the – High flow rate funnels could be sterilized by autoclaving, – High total throughput but this is too laborious for routine use. A disposable sterile funnel in a certified Safe & reliable quality is the ideal solution. – Sterile or individually, sterile packaged – No risk of cross contaminations Description – No leakages due to proven closure The Biosart® 250 Funnel has been specifically technique designed for microbiological and analytical – No holding of hot funnels quality assurance. Biosart® 250 are sterile – Visibility of the complete filtration funnels which allows for fast filtration required in the routine testing of pharma Economical ceutical and cosmetic products, water, food – Ready to connect and easy to use and beverages and other liquids. A Sartorius – Minimal amount of equipment needed Stedim Biotech gridded membrane is placed – Autoclavable (to a limited extend) on a stainless steel filter support. A Biosart® 250 Funnel is simply fitted on and the sample is filtered. The funnel is made of polypropylene and is sufficiently elastic for optimal sealing with a bayonet-type closure. Graduations are marked at 50, 100, 150, 200 and 250 ml for exact sample volumes. The large inner diameter ensures a high flow rate. The conical form allows a thorough rinsing of the system subsequent to filtration. No liquid is retained in the filter funnel. ®

Specifications Material Polypropylene Capacity 250 ml, 50 ml graduations

Filter diameter 47 mm (or 50 mm), prefilter 40 mm Colony Counting | Biosart Filtration area 12.5 cm2 Max. operating pressure Vacuum only Sterilization Ethylene oxide Lot certificates Sterility and performance tests

Biosart® 250 Funnels, ready to use filter funnels, 250 ml, 50 units

Description Order No. Biosart® 250 Funnel, 50 units, 16407--25----ACK individually, sterile-packaged Biosart® 250 Funnel, 50 units, 16407--25----ALK sterile-packaged

Further information available on request f.o.c. Order no. SL-3017-e

37 Colony Counting | Combisart® Systems 38 in quality assurance. Combisart in microbiological analysis or particle count hardware and consumables for your needs system enables you to select the optimal is autoclaving, the Combisart Since the most reliable sterilization method enumeration of micro-organisms by culture". equipment described in the "General Guide to regards to the sterilization methods of the The system is compliant with ISO 8199 with Sterilization the filter. secondary contamination of the underside of station to be sterilely vented. This rules out to be individually controlled and each filter They allows the vacuum for each filter holder is the stainless steel three-way valve (tap). – all types of filter holders and funnels such as: individual system designed to accommodate a high-grade stainless steel manifold or At the heart of the Combisart Description accessories to make your choice easier. modular design and field-proven standard reliability and saves sterilization capacity. autoclaved them. This method increases an entire unit from each workstation and holders, you can simply unscrew them as inserting the membrane filters in the filter a unique advantage for this method. After Individual and Multi-Branch Systems Microsart tion Couplings (more information under could be used together with Quick Connec- are newly Quick Connection Nipples, which The outlet of the – – A special feature of the Combisart Sterile venting the 3- or 6-branch manifolds. For large number of samples, we recommend base 16841 on the top of a suction flask. 1-branch manifold 16844 or the individual of samples, we recommend the use of the for working on a clean bench. For low number manifold ports is particularly advantageous for vacuum tubings. The Sartorius Stedim Biotech Combisart Combisart Funnels 100 and 250, Microsart Ready-to-use units like 100 and 250, Biosart Biosart glass or polycarbonate Autoclavable reusable funnels made of funnels for colony counting Flammable units such as stainless steel ® ® 250 Funnels Combi.jet) or as hose nipples ® – The Sterile Vented Filter Station 1- and 3-branch manifolds The low height of the ® 100 Monitors and Microsart ® ® design offers system is ® features a ® ® @filter system ®

membrane filters, Microsart 47 mm is highly recommended for all 47 mm from when using the Combisart Some of the advantages you will benefit – – will benefit from: 47 mm features additional advantages you Combisart – Stainless steel 304 – long lifecycle – Space-saving in the autoclave – Economical – – Easy pouring out of non-filterable samples – Filtration of 3 or 6 samples in parallel Saves time – – – – Safe & reliable Alternatively to 16840 the Microsart membrane filter surface. enous distribution of the residues on the of the single base 16840 allows a homog- steel funnels. The stainless steel filter support for Microsart membranes 16840 or the Microsart The turnable Maximum flexibility combinations. by using corresponding adapter-| Glass units (16306 or 16307) can be fitted directly. 16511 can be screwed onto the manifold 3 or 6 polycarbonate holders of the type filtration. minimizing the risk of contamination during that the Monitors are positioned perfectly, The Biosart www.sartorius-stedim.com/microbio Filtration systems fast and easy completed at to adapt disposable Biosart 50 mm membranes) the manifolds are flexible In connection with the single base 16840 (for The right equipment for your application individual user funnels can be positioned to suit the handed users in your laboratory, because Filtration equally easy for left- or right from each unit You can pour out a non-filterable sample set-ups Maximum flexibility due to different users Equally easy for right- and left-handed on a clean bench Low height is advantageous for working allow easy cleaning & rinsing Special polished stainless steel surfaces Sterilization acc. to ISO 8199 filtration Sterile venting of each ® ® hardware-setups 100 adapter 16424 ensures ® single base for 50 mm @filter. membrane after ® ® 250 or Funnels and ® System: stopper- ® stainless Base ® Base

Combisart Combisart Microsart Combisart Microsart Optimized Combisart by bayonet closure Combisart Description grade stainless steel, pre-assembled with stainless steel funnels and lids Description steel, without funnels and lids, to accommodate various funnel types fast under: http://www.sartorius-stedim.com/microbio Sterilization Max. operating pressure Dimensions in mm (L|H|D) Stainless steel quality Specifications Parts and materials Flow rate per filter station for water at 90% vacuum Filtration area Combisart Outlet (6-branch) Outlet (1- and 3-branches only) Suitable membrane Inlet (branches only) Outlet spout (individual system) filter diameter Combisart Combisart Combisart Combisart Combisart Combisart Combisart Combisart Combisart Combisart Combisart steel, accommodate stainless steel funnels and Biosart to accommodate stainless steel funnels and Biosart ® ® ® for the use with 47 mm membranes, Click-Fit closure for ® ® ® ® ® ® ® ® ® ® ® ® ® ® Funnel, Microsart Base 47 mm, with frit, stainless steel ® ® and Microsart 6-branch stainless steel manifold, 500 ml 6-branch stainless steel manifold, 100 ml 3-branch stainless steel manifold, 500 ml 3-branch stainless steel manifold, 100 ml 1-branch stainless steel manifold, 500 ml 1-branch stainless steel manifold, 100 ml individual filter holder, stainless steel, 500 ml individual filter holder, stainless steel, 100 ml Single base with frit (for 50 mm membranes), stainless 6-branch stainless steel manifold, without frits 3-branch stainless steel manifold, without frits 1-branch stainless steel manifold, without frit individual base, stainless steel, with frit (50 mm), Hardware Set-Ups – Choose complete filtration systems easy and individual system and multi-branch manifolds, made of high- individual and multi-branch bases, made of high-grade stainless

® Combi.jet

® @filter and other funnel types sealed

Vacuum only 6-branch manifold: 910|103|120 3-branch manifold: 435|103|120 304 High-grade stainless steel: B.S. 304S31|AISI By flaming, By dry heat (max. 180°C), By autoclaving (max. 134°C), By other methods acc. to ISO 8199 Silicone lid seal tap made of stainless steel. Silicone flat gasket. Lid, funnel, base part, filter support, clamp and 200 ml/min with 0.2 µm membrane filter 600 ml/min with 0.45 µm membrane filter 12.5 cm Hose nipple DN 10 (tubings with DN 10 are connectable) Quick Connection Nipple DN 7 Female thread, TR 20 10 mm outer diameter 50 mm (47 mm, if using a 47 mm frit 6980103) 2 (if using stainless steel funnels)

base for ® 100|250 100|250

® 100|250 100|250 +

Capacity 6 6 3 3 1 1 1 1

+ + + + + + + +

500 ml 100 ml 500 ml 100 ml 500 ml 100 ml 500 ml 100 ml

Order No. Order No. 1ZU---0002 16831-CS 16832-CS 16828-CS 16824-CS 16845-CS 16844-CS 16201-CS 16219-CS 16843 16842 16844 16840 16841 39

Colony Counting | Combisart® Systems Colony Counting | Combisart® Systems Replacement parts for Combisart 40 Replacement parts for Combisart stainless steelcap 6980270 16 silicone O-ring 6980271 + 2 mm 3-way tap stainless steel 6980267 knurled nut 6980268 ® ® individual filter holders manifolds (pack of10) Plug conical 6980225 (pack of50) Minisart SRP 17575-ACK single base 16840* with clamp funnel 40ml 6981004 (pack of12) Plug LuerLock 17012-E single base 16840* clamp 6980264 with clamp funnel 100ml 6981065 silicone lidseal 6981064 stainless steellid 6981063 *) Alternativelyto16840theMicrosart 16843 6-branch 16842 3-branch 16844 1-branch funnels: supports and without base Manifolds for all47mmmembranefilters,Microsart ® 6980264 clamp funnel 500mlwithclamp 6981002 silicone lidseal 6981003 stainless steellid 6981001 Base 47mmishighlyrecommended (incl. 50mmfritandsilconegasket) 16840* 6980103 stainlesssteelfrit47m 6980102 stainlesssteelfrit50m 6980235 siliconeO-ring(packof3) 6980104 PTFEflatgasket 6980124 siliconeflatgasket 6980274 16 silicone O-ring 6980271 ® Funnels andMicrosart + stainless steelsinglebase 2 mm silicone O-ring nipple quick connection 1EAS--0026 20 8 + ® @filter units. hose nipple 6980272 Stainless steel frit for Microsart Hose nipple, stainless steel, DN 10 Quick Connection Nipple, stainless steel Stainless steel frit, 47 mm diameter (16840) Stainless steel frit, 50 mm diameter (16840) PTFE flat gasket underneath the frit (16840) Biosart Minisart Stainless steel funnel with Description Biosart with silicone stopper with silicone stopper with silicone stopper stainless steel Glass funnel Adapter, stainless steel Glass funnel Adapter, Funnels, lids, seals and filter holders to connect on the Combisart (1ZU---0002) Silicone flat gasket underneath the frit (16840) Silicone O-ring for manifold female threads (also 1ZU---0002) Silicone O-ring for single base 16840 male thread the 3-way-valve, if sterile venting is not required Plug, conical, to close the venting hole beside if sterile venting is not required Description Plug luer lock, to close the Minisart could be autoclaved 5 times. 0.2 µm, individually sterile-packaged, Accessories and replacement parts for the Combisart Description Stainless steel funnel with Lid seal, silicone Lid, stainless steel closure clamp stainless steel silicone Stainless steel funnel with Lid seal, silicone Lid, stainless steel closure clamp Polycarbonate filter holder, closure clamp Glass filter holder, funnel and metal clamp complete with filter support, Glass filter holder, and funnel complete with filter support Combisart funnel and metal clamp complete with filter support, ® ® 100 Adapter, 100 Adapter, ® SRP25, sterile filter for venting, ® Adapter, to accommodate various funnel types

or onto 16841 (individual base) 16842 and 16843 16842 and 16843 16842 and 16843 onto Combisart 16307 (glass funnel, 250 ml) onto Combisart 16306| Adaptation Biosart Biosart onto 16840 (Combisart onto Combisart

® ® ® Capacity 100 ml 500 ml for 100 ml funnel for 100 ml funnel 40 ml for 500 ml funnel for 500 ml funnel 250 ml 250 ml 30 ml Base 47 mm 15 (glass funnel, 30 ml) 100 Monitors 100 Monitors ® inlet,

® ® ® manifolds 16844, manifolds 16844, manifolds 16844,

single base)

1 1 1 1 1 filter diameter Membrane 1 Quantity 47|50 mm 47|50 10 12 1 3 3 50 47|50 mm 47|50 47|50 mm 47|50 47 mm 47|50 mm 47|50 25 mm

System

6980272 1EAS--0026 6980103 6980102 6980104 6980124 Order No. 1ZU---0001 6980225 17012------E 6980235 6980274 17575------ACK

® system Order No. Order No. 6981065 16836 16837 16835 16424 6981002 6981064 6981063 6981004 6981003 6981001 16511 16307 16306

Colony Counting | Combisart® Systems Microsart® Combi.jet 2-branch stainless steel manifold for microbiological analysis

The Microsart® Combi.jet is a 2-branch Maximum Flexibility manifold, made of high-grade stainless steel. The Microsart® Combi.jet enables you to The manifold has been specifically designed select the optimal hardware and consumables for the use together with the Microsart® e.jet for your needs in microbiological analysis in Transfer Pump. The system is able to create quality assurance. At the heart of the whole sufficient vacuum for vacuum filtration system is the Microsart® Combi.jet, the concomitantly transferring the filtered liquid stainless steel 2-branch manifold, designed directly to waste. Microsart® Combi.jet and to accommodate all types of filter holders Microsart® e.jet can be easily connected and funnels such as: and disassembled by the innovative Quick – Ready-to-use units Microsart® @filter 100 Connection technology. and 250 – Ready-to-use units Microsart® Funnel 100 Compact Design and 250 The complete traditional equipment, such as – Ready-to-use units Biosart® 100 Monitors connectors, tubes, suction flask, protection – Ready-to-use units Biosart® 250 Funnels filter, Woulff’s bottle and a vacuum pump, – Flammable units such as stainless steel requires a lot of laboratory space and is funnels time consuming to operate and maintain. – Autoclavable glass filter holders Microsart® Combi.jet reduces operating – Autoclavable polycarbonate filter holder complexity due to its small and compact design. The Transfer Pump Microsart® e.jet fits Reliability: Ideal for microbiology visually and ergonomically into this design. applications – Sterile venting after filtration Quick Connection – Easy to clean and sanitize Building-up the vacuum filtration system is – Smooth and reliable filtration easy and fast thanks to the innovative Quick Connection Coupling and Nipples at the Economically efficient Microsart® Combi.jet manifold and Microsart® – Saving time due to Quick Connection e.jet Transfer Pump. Simply push-to-connect technology for assembling and pull-to-disassembling the – Saving work space (saves 70%) whole system within seconds. – No need of suction flasks and water traps

Sterile Venting ® Systems

® A special feature of the Microsart Combi.jet manifold are the stainless steel three-way valves (taps). They allow the vacuum for each filter holder to be individually controlled and each filter station to be sterilely vented. This rules out secondary contamination of the underside of the filter. Colony Counting | Combisart

42 Specifications

Stainless steel quality High-grade stainless steel: B.S. 304S31 | AISI 304 Dimensions in mm (L | H | D) 246 | 98 | 130 Max. operating pressure Vacuum only Sterilization By autoclaving (max. 134°C) Parts and materials Manifold: stainless steel, silicone O-ring Quick Connection Coupling PVDF, closure: stainless steel, sealing: FKM | FPM Inlet (manifold) Female thread, TR 20 + 2 Outlet Quick Connection Coupling (female), inner diameter NW 7, non-shut-off

Microsart® Base 47 mm Materials stainless steel, silicone O-ring Suitable membrane 47 mm filter diameter Filtration area (e. g. for the 12.5 cm² use with Microsart® Funnels)

Microsart® Combi.jet 2-branch manifold, made of high-grade stainless steel, without frits and funnels, to accommodate various funnel types

Description Order No. Microsart® Combi.jet 2-branch manifold, without frits 16848-CJ Microsart® Base 47 mm, with frit, stainless steel base for 1ZU---0002 Combisart® and Microsart® Combi.jet Optimized for the use with 47 mm membranes, Click-Fit closure for ® ® Systems

Microsart Funnel, Microsart @filter and other funnel types sealed ® by bayonet closure

Accessories and replacement parts for Microsart® Combi.jet Description Quantity Order No. Minisart® SRP25, sterile filter for venting, 50 17575------ACK 0.2 µm, individually sterile-packaged, could be autoclaved 5 times.

Plug luer lock, to close the Minisart® inlet, 12 17012------E Colony Counting | Combisart if sterile venting is not required Plug, conical, to close the venting hole beside 10 6980225 the 3-way-valve, if sterile venting is not required Silicone O-ring for Microsart® Base 47 mm 3 6980274 male thread Silicone O-ring for manifold female threads 3 6980235 Combisart® single base, stainless steel, optimal 1 16840 for the use with 50 mm membrane filters, funnel closure by bayonet or adapter Microsart® Combi.jet Coupling, Quick 1 1EAS--0022 Connection, PVDF

Funnels and filter holders to connect onto the Microsart® Combi.jet manifold are equivalent to those for the use with the Combisart® system (page 38).

43 How to set-up a vacuum filtration system

Microsart® Combi.jet 2-branch Stainless Steel Manifold plus Microsart® e.jet

The filter stations are directly connected to a Transfer Pump for simultaneous transfer of the filtrate to waste. Easy assembling thanks to Quick Connection technology.

Order Information

Pos. Description Order Order No. Detailed Qty. Information on page Microsart® Combi.jet stainless steel equipment: 43 1 Microsart® Base 47 mm 2 1ZU---0002 2 Microsart® Combi.jet 2-branch manifold 1 16848-CJ Systems ® Sterile venting of the filter station: 41 3 Minisart® SRP25, 0.2 µm 1 17575------ACK 4 Silicone tubing, pressure-sided, 1 m 2* 1ZAS--0007 54 Vacuum Pump: 53 5 Microsart® e.jet Transfer Pump, 230 V, 50 Hz 1 166MP-4 Additional accessories: Microsart® @filter 100, sterile filter units,

Colony Counting | Combisart packaged on trays 1 16D01--10-H6--TG 33 Stainless steel tweezers 1 16625 57 Colony Counter 1 17649 56 Incubator 1 18113 56 Container for anaerobic incubation 1 16671 57

* required length depends on distance between Transfer Pump and drain

44 Combisart® 1-branch Stainless Steel Manifold plus Microsart® mini.vac

1 8 9 5 2 4 6 3

The filter station is connected to a suction flask, which is connected to a filtrate-protected vacuum pump.

Order Information

Pos. Description Order Order No. Detailed Qty. Information on page Combisart® stainless steel equipment: 38 1 Combisart® single base, 50 mm 1 16840 2 Combisart® 1-branch manifold 1 16844 Sterile venting of the filter station: 41 3 Minisart® SRP25, 0.2 µm 1 17575------ACK 4 Rubber vacuum hose, 1 m 3* 16623 51 Systems ® Suction flask and stopper: 50 5 Tube connector 1 17204 6 Silicone stopper 1 17173 7 Suction flask, 2 liters 1 16672 Water trap for pump protection: 51 8 Vacusart®, 0.45 µm 1 17804------M Vacuum Pump: 52 9 Microsart® mini.vac, 230 V, 50 Hz 1 16694-2-50-06 Colony Counting | Combisart Additional accessories: Microsart® e.motion Dispenser 1 16712 14 Stainless steel tweezers 1 16625 57 Colony Counter 1 17649 56 Incubator 1 18113 56 Stainless steel prefilter attachment 1 16807 57 Container for anaerobic incubation 1 16671 57

* required length depends on distance between the filter station and the vacuum source

45 Combisart® 1-branch Stainless Steel Manifold plus Microsart® e.jet

The filter station is directly connected to a vacuum fluid pump for simultaneous transfer of the filtrate to waste. Easy assembling thanks to Quick Connection technology.

Order Information

Pos. Description Order Order No. Detailed Qty. Information on page Combisart® stainless steel equipment: 38 1 Combisart® single base, 50 mm 1 16840 2 Combisart® 1-branch manifold 1 16844 Sterile venting of the filter station: 41 3 Minisart® SRP25, 0.2 µm 1 17575------ACK 4 Silicone tubing with Quick Connection Systems ® Coupling, 20 cm, vacuum-sided 1 1ZA---0006 54 Vacuum Pump: 53 5 Microsart® e.jet Transfer Pump 1 166MP-4 6 Silicone tubing, pressure-sided 1 m 2* 1ZAS--0007 54 Additional accessories: Microsart® e.motion Dispenser 1 16712 14 Stainless steel tweezers 1 16625 57 Colony Counter 1 17649 56 Colony Counting | Combisart Incubator 1 18113 56 Stainless steel prefilter attachment 1 16807 57 Container for anaerobic incubation 1 16671 57

* required length depends on distance between vacuum source and drain

46 Specifications Made of Stainless Steel, Glass and Polycarbonate Traditional Sterilization Max. operating pressure in mm (W|H|D) Filtration area for water at 90% vacuum Flow rate per filter station Parts and materials Dimensions Stainless steel quality Stainless steel multi-branch manifolds and individual filter holders by flaming. Funnel and filter support can be disinfected the residues on the membrane filter surface. steel frit allows homogenous distribution of turned on and off individually. The stainless fold ports, the vacuum for each holder can be Due to the stainless steel taps on the mani- mass examinations have to be carried out. or six separate filter holders save time when 100 The manifold systems are available with Multi-branch manifolds to the ease of use. the addition or removal of the funnels adding and off. The special closure clamps simplify the base allow the vacuum to be turned on routine examinations. Stainless steel taps in Simple handling is very important regarding a uniform distribution of the residues. The stainless steel frit filter support ensures the membrane filter surface are of interest. particles or microorganisms retained on specifically for applications in which the 100 only in the funnel capacity (either 40 ml, The three stainless steel holder types differ Individual filter holders Suitable membrane Outlet (branches only) (individual system) Outlet spouts filter diameter

ml or 500 ml capacity funnels. The three ml or 500 ml). They have been designed

Multi-Branch Manifolds and

Vacuum or max. 2 bar pressure (29 psi) 6 6 6-branch manifold: 3 3 12.5 cm 600 ml/min with 0.45 µm membrane filter 200 ml/min with 0.2 µm membrane filter of stainless steel. Silicone flat gasket. Silicone lid seal Lid, funnel, base part, – filter support, clamp and tap made By other methods acc. to ISO 8199 By flaming, By dry heat (max. 180°C), By autoclaving (max. 134°C), 3-branch manifold: 304 B.S. 304S31|AISI High-grade stainless steel: Hose nipple, DN 10 10 mm outside diameter filter support 6980103) 50 mm (47 mm, if using a 47 mm frit + + + + 500 ml: 916|329|132 100 ml: 906|268|120 500 ml: 442|262|132 100 ml: 432|184|120

(max. 121°C). devices can be sterilized by autoclaving manifold e. g. Combisart flask. It is used on a suction flask or a vacuum 16511 is like 16510, but without receiver mended for highest standing times). Type with slight overpressure (0.5 bar is recom- and can be operated with vacuum as well as Type 16510 is complete with receiver flask, Polycarbonate filter holders of retained residue. The glass frit ensures uniform distribution (max. 134°C) or by dry heat (max. 180°C). top part. They can be sterilized by autoclaving part and for larger volumes with a 250 ml filtration of small volumes with a 30 ml top These filter holders are available for the Glass filter holders

Individual

Filter Holders ® systems. Both

Colony Counting | Traditional Filter Holders Colony Counting | Traditional Filter Holders Replacement parts for traditional manifolds Replacement parts for traditional individual filter holders 48 Individual stainless steel filter holder, 100 ml Description funnels and lids Individual stainless steel filter holders, pre-assembled with stainless steel Individual stainless steel filter holder, 500 ml Individual stainless steel filter holder 3-branch stainless steel manifold, 100 ml Description Multi-branch manifolds, stainless steel, with stainless steel funnels and lids without lid, 40 ml 3-branch stainless steel manifold, 500 ml 6-branch stainless steel manifold, 100 ml 6-branch stainless steel manifold, 500 ml Glass filter holder, Description Glass filter holders Glass filter holder, funnel and metal clamp complete with filter support, Polycarbonate filter holder, Description Polycarbonate filter holder funnel and metal clamp complete with filter support, Polycarbonate filter holder, or pressure filtration receiver flask, for vacuum with 250 ml top part and for vacuum filtration only with 250 ml top part,

30 ml Capacity 250 ml 250 ml Capacity 250 ml

1 Capacity 1 1 3 Capacity 3 6 6 25 mm Membrane filter diameter 47|50 mm 47|50 Membrane 47 mm filter diameter 47 mm + + + + + + + 100 ml 500 ml 40 ml 100 ml 500 ml 100 ml 500 ml

Order No. 16219 16201 16220 Order No. 16824 16828 16832 Order No. 16831 16306 Order No. 16307 16510 16511

Colony Counting | Traditional Filter Holders Colony Counting | Accessories 50 Silicone stopper Silicone stopper Silicone stopper Description Order numbers for bored stoppers for suction flask 2 liters 16672 Hose barb, complete, Polypropylene, for suction flask 2 liters 16672 Assembling kit for hose barb for suction flask 5 liters 16672-----1 Bored stopper 75 D for suction flask 5 liters 16672-----1 Glass tube for silicon stopper 75 D for suction flask 5 liters 16672-----1 Description Replacement parts for suction flasks restrictions on glass hose nipples) Suction flask, 1 liter (not available in countries which have safety Suction flask, 5 liters acc. to DIN 12476, incl. stopper 75 D Description Order numbers for Suction flasks restrictions on glass hose nipples. countries which do not have safety A 1-liter capacity flask is available for opening, 60 mm. Stoppers are not enclosed. hose nipple, 9 mm. Inner diameter of the Standard No. 12476. Outer diameter of the according to the – German Industrial 50 glass with plastic safety hose nipple Vacuum-resistant flask made of duran Suction flask, 2 liter capacity Suction flasks and stoppers Accessories for Vacuum Filter Holders and Manifold Systems Silicone stopper a Vacusart manifold to a suction flask 1 or 2 liters (not necessary when Tube connector for connecting a Combisart Suction flask, 2 liters acc. to DIN 12476, without stopper and glass tube Silicone stopper Silicone stopper Description Order numbers for bored stoppers for suction flask 1 liter 16606 ® is connected directly to the bored stopper)

onto the suction flask 16606 filter holders (16201, 16219, 16220) or other individual stainless steel 16307|16 (glass funnel, 250 ml) 16307|16 onto the suction flask 16606 (glass funnels, 30 ml) 16306|15 onto the suction flask 16606 Combisart Adaptation onto the suction flask 16672 filter holders (16201, 16219, 16220) or other individual stainless steel 16306|15 (glass funnels, 30 ml) 16306|15 onto the suction flask 16672 Combisart Adaptation onto the suction flask 16672 16307 (glass funnel, 250 ml) ® ®

individual base 16841 individual base 16841

® stainless steel

Order No. 1EAQ--0017 6983003 1EA---0018 1EAS--0019 Order No. 16606 Order No. 17004 17173 17005 17006 17204 16672-----1 16672 Order No. 17174 17175 Vacusart Description the rubber hose to the vacuum pump. the bored stopper and connected with It could be put directly into the hole of for the protection of vacuum pumps. 0.45 µm. Vacusart PTFE membrane with a pore size of and a hydrophobic, but air-permeable unit, consisting of a polypropylene housing Vacusart filtrate into an electric vacuum pump source, in order to prevent overflow of Used between suction flask and vacuum Water traps Vacusart Woulff’s bottle, 500 ml Description from overflowing from the suction flask. and prevents furthermore the filtrate with glass units without a separate tap source. Allows simple control of the vacuum Used between suction flask and vacuum Woulff’s bottle, 500 ml Rubber vacuum hose (1 meter) Description state length required in meters. vacuum pumps, etc. When ordering, please the system components, e. g. suction flasks, Thick-walled rubber hose for connecting Rubber vacuum hose (1 meter) ® ® ® water trap, is a ready-to-connect filtration

® is perfectly suitable pack of 3

Order No. Order No. 17804------M 16610 Order No. 16623 51

Colony Counting | Accessories Colony Counting | Accessories 52 Microbiology laboratory environment. up to date technology for daily use in the The new vacuum pump series provides sources of vacuum. level, oil- and maintenance-free; reliable Neoprene membrane pumps with low noise Microsart Microsart Electric vacuumpumps Specifications of electric vacuum pumps Noise level [100 mbar] Ultimate Vacuum Delivery

Fine adjustment head for 16694-2-50-06 and -1-60-06, Fine adjustment head for 16694-2-50-06 and -1-60-06 Fine adjustment head for 16694-2-50-22 and -1-60-22 Sound absorber for 16694-2-50-06 and -1-60-06 Sound absorber for 16694-2-50-22 and -1-60-22 of one membrane, two valve springs and two head seals Replacement kit for 16694-2-50-06 and -1-60-06, set of one membrane, two valve springs and two head seals Replacement kit for 16694-2-50-22 and -1-60-22, set Replacement parts Microsart Microsart Microsart Materials (contact with Operating Pressure for pressure filtration Microsart Description Order numbers Recommended application (mm) Dimensions W|H|D Weight Operating Current Power P1 Motor Protection Mains Ambient Temperature Connectors for Tube (mm) filtrate possible) ® ® ® ® ® ® mini.vac up to 3 filter stations in parallel, 115 V, 60 Hz mini.vac up to 3 filter stations in parallel, 230 V, 50 Hz maxi.vac for multiple filtration runs, 115 V, 60 Hz maxi.vac for multiple filtration runs, 230 V, 50 Hz maxi.vac mini.vac

Microsart 100 mbar 22 l/min 16694-1-60-22 16694-2-50-22 1 bar 57.5–59.0 dBA All multi-branch manifolds 261|204|110 7.1 kg 0.9 A 130 W IP 44 Hz 16694-1-60-22: 115 V|60 Hz 16694-2-50-22: 230 V|50 5…40°C ID 9 NBR (Perbunan) Aluminum, CR (Neoprene),

® maxi.vac recovery rates and shorter incubation times. which results in decreased sub lethals, higher a membrane filter under better conditions, bacteria) are concentrated on the surface or specifications. Thus damageable cells (e.g. controlled and can be easily adjusted to your The vacuum produced by the new pumps is

100 mbar 6 l/min 16694-1-60-06 16694-2-50-06 Microsart 2.5 bar 53.5 dBA 3-branch manifolds Single filtration run up to 164|141|90 1.9 kg 0.63 A 65 W IP 20 Hz 16694-1-60-06: 115 V|60 Hz 16694-2-50-06: 230 V|50 5…40°C ID 4 PPS, EPDM, FPM (Viton)

® 1EV---0001 1EV---0002 1EH---0001 1EH---0002 1ED---0054 1ED---0055 Order No. 16694-1-60-06 16694-2-50-06 16694-1-60-22 16694-2-50-22 1EV---0003 Order No. mini.vac

to operate and maintain. laboratory space and is time consuming traditional equipment requires far more filtrate collection container. The complete the vacuum must be broken to empty the and a vacuum pump. After several samples containers, protection filter, Woulff’s bottle parts including connectors, tubes, vacuum brane Filtration Method consists of numerous Until now vacuum equipment for the Mem- Reducing operating complexity guarantee smooth and reliable filtration. flow rates and a defined maximum vacuum placement by air in one minute). Constant of > 4.0 Nl/min (4.0 Normliters water dis- pressure of 600 mbar and a higher flow rate Microbiology achieving a trans membrane e.jet is ideal for sample preparation in filtration bench. or Woulff’s bottles from the laboratory will eliminate the need for side-arm flasks The with Quick waste. The second generation of transferring the filtered liquid directly to for vacuum filtration tory pump able to create sufficient vacuum Microsart Inlet Protection class Protection type Max. viscosity Max. Temp of liquid Max. ambient Temp. Dimensions (W|L|H) Weight Materials (in contact with filtrate) Mains Max. Pressure Max. Vacuum Flow rate Technical Specifications Microsart | Outlet ® e.jet Transfer Pump Connection ® e.jet is a new vacuum labora- and concomitantly Microsart Microsart ® e.jet

Quick Connection on hose nipples for DN 10 tubings III IP 64 cSt <150 +5…+80°C +5…+40°C 120 Power supply: 202.8 g Pump: 1425.3 g PTFE, ETFE, Polypropylene, EPDM, POM, PSU Hz 100–240 V|50-60 1.0 bar 0.4 bar > 4.0 Nl/min

170 from when using the Microsart Some of the advantages you will benefit The Quick Connections are non-shut-off. sembling the whole system within seconds. to-connect for assembling and pull-to-disas - hose nipples for DN 10 tubings. Simply push- assembled to Quick Connection Couplings on is pump head. The ficient vacuum, when liquid is drawn into the efficiency and capability to generate suf- Traditional vacuum pumps often loose their Laminar Flow Cabinets. in particular less congestion working in only 30% of the average space meaning e.jet – – – easy and fast thanks to the innovative Quick Building-up the vacuum filtration system is Quick Connection drawn into the pump head. loss of efficiency or malfunctions from water to pump both gas and liquids, meaning no pared to traditional equipment Microsart ry Connections. The Microsart The + money – that’s economic efficiency Saving 70% of work space while saving No need of suction flasks and water traps Ideal for microbiology applications equipped with Quick Connection Nipples for manifolds up to 3 filter stations. Com- 190 mm Microsart and a stainless steel manifold require ® e.jet pump is an ideal accesso- Microsart ® ® e.jet is designed e.jet Transfer Pump ® e.jet

Colony Counting | Accessories Microsart® e.jet with Quick Connection

1 5

2 3 4

Order Information

Description Order No. in Number picture Microsart® e.jet Transfer Pump with Quick Connection, 166MP-4 1 without tubings, inlet and outlet hose nipples for DN 10 tubings

Accessories Order No. in Number picture Tubing with Quick Connection Coupling (PSU), silicone, 1ZA---0006 2 20 cm, for vacuum-sided connection, inner diameter DN 10, outer diameter DN 20, wall thickness 5 mm (when ordering, please state length required in meters) Silicone tubing, 1 m, for pressure-sided connection, inner 1ZAS--0007 3 diameter DN 10, outer diameter DN 14, wall thickness 2 mm

Replacement Parts

Description Order No. in Number picture Pump head complete for 166MP-3 and 166MP-4 1EP---0001 Colony Counting | Accessories Power supply complete for 166MP-3 and 166MP-4 1EE---0007

Threaded fittings Quick Connection set, 2 Nipples (POM) on R3/8" male 1EAS--0027 4 thread and 2 Couplings (PSU) on DN 10 hose nipple Quick Connection Nipple, stainless steel 1EAS--0026 5 DN 10 hose nipple on R3/8" male thread 1EAF--0020

54 Order numbers traditional pumps

Description Order No. Multiple filtration runs: 16612 13 mbar final vacuum, 26 l/min max., 220 V, 50 Hz Multiple filtration runs: 16615 13 mbar final vacuum, 26 l/min max., 110 V, 60 Hz Individual filtration run: 16692 100 mbar final vacuum, 20 l/min max., 220 V, 50 Hz Individual filtration run: 16695 100 mbar final vacuum, 20 l/min max., 110 V, 60 Hz

Replacement parts Order No. Set of two neoprene membranes, four valve springs 6986017 and two neoprene head seals for 16612/16615 Set of one neoprene membrane, two valve springs 6986105 and one neoprene head seal for 16692/16695

Water jet pump Simple vacuum source. For connection to a water tap with G3/4 male thread.

Description Order No. Water jet pump, with G 3/4 female thread 16611

Hand-operated vacuum pump Practical vacuum source, also outside of a laboratory. Up to 80% vacuum can be obtained. The body is of PVC. Supplied completely with gauge, vacuum release lever and a 60-cm length of clear plastic tubing.

Description Order No. Colony Counting | Accessories Hand-operated vacuum pump with gauge 16673

Dosing Syringe The most convenient way to moisten the NPS with water is to use a dosing syringe with an adapted Minisart® syringe filter. Simultaneous sterilization and dispensing of demineralized water in 3.5 ml steps is easily done by dropping the sinker at the end of the suction tubing into the water, then filling the dosing syringe and dispensing sterile water by operating the twigger automatically.

Description Order No. Dosing syringe, 0.5–5 ml 16685-2 Minisart®, 0.2 µm, individually, sterile-packaged 17597------K Replacement part: tubing with sinker for 16685-2 and 16685 6986125 Service Kit for Dosing Syringe 16685-----2 1EP---0002

55 Colony Counting | Accessories 56 Incubator Description Capacity Spacial temperature deviation Temperature deviation Temperature range Dimensions W|H|D (mm) Dimensions W|H|D Max. load for insertion plate Weight Rated power Frequency Voltage Incubator Colony counter Description marker refill. counter is supplied with an additional pen, and has a 4-digit LCD-display. The counter, it is as simple to use as a ball-point Compact, handy battery-operated colony Colony counter Specifications Replacement part: Black marker refill 60 mm or 72 petri dishes: 200 to hold the following numbers and sizes of has a capacity of 15 liters and is designed pads or other nutrient media. The incubator incubation of membrane filters on nutrient Compact, space-saving incubator for the Incubator

90 mm. Incubator

+ 47 mm or 160

+ 56 mm| approx. 15 liters Less than ±0.8°C Less than ±0.2°C (at 37°C and RT 20°C) 20°C (or 5°C above room temperature) to 50°C Outer 340|270|431 Inner 270|205|288 5 kg (12 lbs) 5.5 kg (12 lbs) 0.2 kW 50|60 Hz 50|60 230 V 18113

into the chamber. cover is opaque, avoiding light penetration plate simplify loading and unloading. The The swing-up cover and removable insertion

18113 Order No. Order No. 17649 6981540 Membrane filters should only be handled Stainless steel tweezers Stainless steel prefilter attachment Description or being hidden by, an excess of particles. the filter surface without disturbance from, is incubated, and the colonies can grow on After filtration is complete, the test filter retentive membrane filter (e. g. 0.45 µm). on the surface of the underlying bacteria- to pass through. These microbes are trapped sample, whereas it allows microorganisms the coarse suspended particles from the 8 µm is used as the prefilter and it retains ester) membrane filter with a pore size of 11301, a white holders. It can be autoclaved and flamed. and base of the stainless steel vacuum filter tration. The device is clipped between funnel and during the actual bacteria retentive fil- samples for microbiological analysis before the removal of coarse, solid particles from The stainless steel prefilter holder allows Stainless steel prefilter attachment Stainless steel tweezers Description careful, firm hold of the membrane filter. clavable. They have blunt-edged tips for a tweezers can be flamed and they are auto Sartorius Stedim Biotech stainless steel nation which can result from hand contact. with suitable tweezers to avoid contami individually, sterile packaged and 8 µm pore size for the prefilter holder, pack of 100, Cellulose nitrate membranes with 50 mm diameter Replacement part: support plate, autoclavable, flammable Anaerobic container Description dishes. fourteen 60 mm, or up to six 90 mm petri vacuum gauge and sealing ring. For up to inert gas, with 6 mm hose nipples (for 16623), the vacuum exhaust and for cleaning with petri dishes. The plastic lid holds two taps for insert for convenient insertion and removal of diameter, 10.7 cm depth and a with metal Stainless steel container with 11.8 cm inner Container for anaerobic incubation

cellulose nitrate (cellulose

Order No. 16625 Order No. 16807 11301--50----ACN Order No. 6981139 16671 57

Colony Counting | Accessories Colony Counting | School Kit 58 testing. equipment necessary for microbiological The rugged aluminum case contains all the in schools and other educational institutes. microbiology and environmental protection is an ideal teaching aid for instruction in The school kit for microbiological experiments complete kit. testing, we recommend our practical, For specific applications in microbiological Complete kit School Kit for Microbiological Experiments 24002 Order number Endo nutrient pad sets Standard nutrient pad sets Wort nutrient pad sets Culture media (nutrient broth) Inoculation loop sterile water Filtration system for Filtration system for samples Stainless steel tweezers Aluminum case Parts supplied Contents

in a lockable School kit for microbiological experiments, 14053 14055 14058 14132------K 17109 Membrane filter 11307-025N. Filter holder 16517E. Syringe 16647. Glass fiber filter 13400-013S. Adapter 17108D. Syringe 16647. Device 16510. 3-way valve 16639. 16625

and a 3-way valve. filtration, is created with help of a syringe The vacuum, which is necessary for the growth at different temperatures. through alcoholic fermentation; and bacterial on substrates in nature; production of gas effects of antibiotics; detection of yeasts microorganisms in water, air, and soil; the of methods for 7 experiments: detection of general instructions and detailed descriptions The handbook included in the case provides aluminum case

Order no. Ordering Information display and user software Upgraded version 16420 with Basic version 16419 Weight 16419 for bottles, container Pump with holding ring Pump Dimensions Power consumption Frequency Power requirements Pump flow rate Further accessories are available on request. 1ZE---0050 1ZE---0040 1ZE---0039 1ZG---0025 16420 1ZG---0014 1ZE---0033 Order number Accessories most clean room benchtops and isolators. space-saving footprint – a great benefit for of isolators. Its low, compact design has a installed countersunk in the working surface clean rooms, integrated into clean benches, or and user software. The pump can be used in as an upgraded version 16420 with display in two versions: as basic version 16419 and The new Sterisart of the final product batch. you are required to supply proof of sterility surface. As a manufacturer of such products, that otherwise enter the body below the skin that are injected into the blood stream or complete sterility of pharmaceutical products International pharmacopeias require the Technical specifications for Sterisart Specifications Sterisart Sterility Testing Systems

Universal Pump ® Universal Pump is available

approx. 14.6 kg approx. 13.5 kg Sterisart Description approx. 440 approx. 336 100 W Hz 50–60 VAC 100–240 ml/min 70–650 Installation kit for isolators Communication kit Transport trolley fixation-slots) of new Millipore Equinox pump for draining (with two slightly different diameters of the Adapter for Sterisart for draining of Millipore Equinox pump Sterisart Adapter for Sterisart Footswitch Description software with display|user ® Universal pump ® ® Universal pump, Universal pump, basic

+ + 365 260 Order no. SLD1003-e, SLD2010 Special brochures available on request. – – Modular design – Compact and ergonomic construction – Robust and maintenance free – Additional Features and Benefits recognition) sequences can be logged; barcode (operator-guided menus; all process Pump available with special software enhanced safety Closed system – no ventilation for + + 485 mm (W 210 mm (with lever) (W ® ® NF units, fitting into container NF units, fitting into container upgraded version + version D + H) + D + H)

Sterility Testing | Sterisart® Universal Pump Sterility Testing | Sterisart® NF 60 S--2019-e, SLD2009-e, SLD2011-e SLD2006-e, SLD2005-e, SLD2007-e, Order no. SLD1002-e, SL-2019-e, Special brochures available on request. environment. tion of the filters without any contact to the are filled with media and used for incuba- units, and after rinsing, the filtration units pump transfers the sample into the filtration positive results is eliminated. A peristaltic risk of a secondary contamination and false of manipulating the filters. By this the main method, however it eliminates the procedure products. It is based on the membrane filter for the sterility testing of pharmaceutical Sterisart Sterisart Sterility Testing Systems ® NF is a completely closed system ® NF

– – – and benefits Sterisart – Secure: – Product-| available – User-friendly, several practical adapters – Easy-to-read graduated marks – Pre-installed color-coded tube clamps Easy to use: – High mechanical stability – Low adsorption – High retention of microbes Reliable, protection against sterilants Gas-impermeable packaging for ® NF offers the following features Sartochem lot number identification ® membrane:

Medical LVPs Pore size of the Filter area membrane filter Type of sterile packaged, ETO-sterilized, needles made of flamable stainless steel, pack size 10 Technical specifications for Sterisart Specifications Sterisart Ordering Information Sterilization Max. operating temperature Max. operating pressure Sample container capacity Pore size of the air filters Flow rate (for water) devices SVPs LVPs sample

® NF alpha disposable units for sterility testing in clean rooms, individually, Tubing systems and bottles), collapsible bags Open containers (i.e. Luer Lock connectors bags with Luer or glass ampoules, glass with septum Closed glass bottles container Type of sample Sartochem ®

15.7 cm 0.45 µm, tested with Serratia marcescens ETO (ethylene oxid gas) or gamma irradiation 50°C 3 bar (approx. 44 psi) at 20°C 120 ml (graduation for the retention of B. diminuta 0.2 µm PTFE, validated acc. to HIMA 500 ml/min at 1 bar (approx. 15 psi) Sterisart Sterisart needle needle and sterile venting needle Luer Lock connection, needle, dual-needle spike, sterile vented Sterisart Description ® NF 2 in each Sterisart inclusive sterile venting ® ® ® NF alpha with Luer or NF alpha with long NF alpha with long marks at 50, 75 and 100 ml) ® container inclusive long

16468------ACD 16467------ACD 16466------ACD Order No. 61

Sterility Testing | Sterisart® NF Sterisart® NF gamma disposable units for sterility testing in isolators, individually sterile, double-packaged, gamma irradiated, needles made of flamable stainless steel, pack size 10

Type of Type of sample Description Order No. sample container LVPs Closed glass bottles Sterisart® NF gamma with long 16466------GBD with septum dual-needle spike, sterile vented SVPs Closed glass vials Sterisart® NF gamma with short 16476------GBD with septum dual-needle spike, sterile vented LVPs, Closed plastic Sterisart® NF gamma with long 16477------GBD SVPs, containers|vials| needle, side opening, with solid eye drops ampoules, plastic pointed tip, non-coring, containers of Blow- inclusive sterile venting needle Fill-Seal fillings LVPs Open containers Sterisart® NF gamma with long 16467------GBD SVPs (i.e. glass ampoules, needle, inclusive sterile venting glass bottles), needle collapsible bags Lyophilisates, Closed glass vials Sterisart® NF gamma with two 16475------GBD soluble with septum dual-needle spikes of different powders, length, one is sterile vented liquid antibiotics Pre-filled Syringes with and Sterisart® NF gamma with 16469------GBD syringes without needles syringe-adapter and long dual-needle spike, sterile vented Medical Tubing systems and Sterisart® NF gamma with Luer 16468------GBD devices bags with Luer or or Luer Lock connection, inclusive Luer Lock connectors long needle and sterile venting needle

NF ®

® NEW Containers|bags Sterisart NF gamma with female 16478------GBD Medical with Luer Lock male Luer Lock connection devices connectors

Sterisart® NF gamma Septum, disposable units for sterility testing in isolators, Sterisart® NF containers with integrated septum for reliable sterile sample taking individually sterile, double-packaged, gamma irradiated, needles made of flamable stainless steel, pack size 10 Sterility Testing | Sterisart LVPs Closed glass bottles Sterisart® NF gamma Septum with 16466------GSD with septum long dual-needle spike, sterile vented LVPs Open containers Sterisart® NF gamma Septum with 16467------GSD SVPs (i.e. glass ampoules, long needle, inclusive sterile glass bottles), venting needle collapsible bags Lyophillisates, Closed glass vials Sterisart® NF gamma Septum with 16475------GSD Soluble with septum two dual-needle spikes of different powders, length, one is sterile vented Liquid antibiotics Pre-filled Syringes with and Sterisart® NF gamma Septum with 16469------GSD syringes without needles syringe-adapter and long dual-needle spike, sterile vented

62 Accessories

Application Description Order No. Difficult-to-dissolve powders in Sterisart® NF gamma tubing system 16470------GBD closed glass vials with septum, with two dual-needle spikes of non-vacuo different length, needles made of flamable stainless steel Sterile venting of containers with Needle with venting filter, 4 cm, 16596------HNK rinsing solution and nutrient media, stainless steel, individually sterile additional sterile venting needles, packaged, gamma irradiated, equal to the inclusive needles pack size 50 of the Sterisart® NF units i.e. type 16467, 16468 and 16477 Further units (16464------ACD|GBD) on request. NF ® Sterility Testing | Sterisart

63 Sterility Testing | Reusable System 64 Support stand Clamp holder Ampoule breaker Holding tongs Inlet tube Description (for ampoule testing) Additional accessories for reusable sterility test system Venting filters, pack size 50 Tube clamps (tubing clips) Incubation rack Holding rod for inlet tube| Silicone tubing, Peristaltic pump Silicone adapter Filling cap with filling needle T-distributor for 2 filter holders Stainless-steel adapter Stainless steel manifold Filter holder with 130 ml capacity Filtration area Filter diameter Sealing Material Specifications of the filter holders according to requirements. user, and the membrane filter can be chosen be designed according to the needs of the watersafe and autoclavable. The system can The filter holders are easy to clean, dish- testing of injection and infusion solutions. Reusable sterility test system for the sterility Reusable Sterility Test System Description General accessories for the reusable sterility test system Sterilization Operating pressure Capacity

4 + 1.5 mm needle 12.5 cm 47 mm Silicone O-ring, 40.5x 3.5 mm (6980574) Silicone gasket, 36/47 mm (6980573) anodized aluminum closing cap. Glass cylinder; polypropylene base and sealing plug; Autoclaving at 121°C Vacuum only at the 115-mm level). incubation at a level of 60 mm, 110 ml up to the mark 16523: 130 ml (56 ml up to the mark for aerobic 2

16970 Order numbers 16976 16969 16973 16963 Order numbers 17574------K 16978 16975 16974 16699 16696 16968 16967 16966 17756 16826 16523

Inlet needle (short) Inlet needle (long) Description (for testing infusion solutions in bottles) Additional accessories for reusable sterility testing system Order numbers for the reusable sterility test system Consumables (membrane filters, 47 mm, 100 pieces/pack) 11306--47------N

13106--47----HCN

11106--47------N

13506--47----HCN

18406--47------N

11407--47------N

13107--47----HCN

11107--47------N

13507--47----HCN

18407--47------N

16696 Order number EMC Directive Low Voltage Directive Machinery Directive Standards Noise Weight Storage temperature range Operating temperature Power rating Speed control ratio frequencies Operating voltages and Rotor speed Specifications Peristaltic pump

Pore size 0.45 µm

0.45 µm

0.2 µm

Description Cellulose nitrate membrane filter Cellulose nitrate membrane filter with hydrophobic edge Cellulose acetate membrane filter Cellulose acetate membrane filter with hydrophobic edge Regenerated cellulose membrane filter Cellulose nitrate membrane filter Cellulose nitrate membrane filter with hydrophobic edge Cellulose acetate membrane filter Cellulose acetate membrane filter with hydrophobic edge Regenerated cellulose membrane filter 89/336/EG EN 50081-1/EN 50082-1 73/23/EG EN 61010-1 98/37/EG EN 60204-1 IEC 335-1, EN 60529 (IP31) <70 dBA at 1 m 5.5 kg| –40°C to 70°C 4°C to 40°C 100 VA 147:1 110–240 V 50/60 Hz 1.5–220 rpm 12.1 lbs

16964 Order numbers 16964-----3 Application pH 4-8, most hydrocarbons pH 4-8, most hydrocarbons pH 4-8, most alcohols, hydrocarbons and oils pH 4-8, most alcohols, hydrocarbons and oils pH 3-12, solvent-resistant pH 4-8, most hydrocarbons pH 4-8, most hydrocarbons pH 4-8, most alcohols, hydrocarbons and oils pH 4-8, most alcohols, hydrocarbons and oils pH 3-12, solvent-resistant

Sterility Testing | Reusable System CONFIDENCE® Validation Services 66 CONFIDENCE Regulatory Compliance requirements. management in order to meet regulatory the sterility test is essential for the quality interpretation of the results. Validation of must be adhered in order to achive reliable trained personnel and validated methods procedure where aseptic conditions, well ceutical product release. It is a very exacting Sterility testing is a critical step in pharma- are applied for the testing procedures. tered laboratories. cGMP recommendations dure is conducted in certified and FDA regis- The validation of the sterility testing proce- product formulation. sample preparation based on your actual to define relevant test conditions for your industry standards. We are working with you tion of current regulatory requirements and CONFIDENCE ® provides the closest interpreta- ® Validation Services

quality systems. to cGMP recommendations and internal Full traceability is available according with the health authority requirements. assessment are provided. They are in line rationale, test results and method transfer Detailed protocols and reports including Documentation pharmacopoeia. product sample according to international Performance of the sterility test of the Sterility test and controlled environment. is conducted under routine testing conditions the product sample. This "positive control" Microbial growth should not be inhibited by Growth promotion of the Sartochem product sample. In addition the filter integrity connections, tubing and containers) to the of all Sterisart Demonstration of the chemical resistance Chemical compatibility study Procedure Validation of the Sterility Testing ® NF' plastic parts (needles, ® membrane is determined.

– – – – – – Practical Exercises – – – – Theoretical Aspects for BeverageIndustries Advanced Course – – – – Practical Exercises – – – – – Theoretical Aspects of ProductSafetyandIndustrialHygiene Microbiological Basics and beverage industry. in the pharmaceutical industry and food quality assurance and| staff members working in the areas of These training seminar are intended for independently of products. for other manufacturers‘ equipment and Biotech projects, but also are available offered in conjunction with Sartorius Stedim spectrum of services. These are not only Biotech offers you a truly comprehensive As a capable service partner, Sartorius Stedim and services are effectively intermeshed. It is possible to add value only if products We think in processes. EXPAND Differentiation ofbacteriaandyeast: and molds Microscopic examinationofbacteria,yeast Industrial Hygiene Concept (HACCP) Hazard AnalysisandCriticalControlPoints Biochemical differentiation detection methods Product-spoiling microorganisms| Evaluation ofdifferentgrowthsamples samples water, particulatemedia,oil-containing Sample filtrationrunswithvariousmedia: Pour plate,streakplate Introduction tomicrobiologicalwork Personnel hygiene drinking water:regulationsundmethods Microbiological examinationofwaterand The microbiologicallab detection methods Growth conditions|microbiological Introduction togeneralmicrobiology Use ofdifferentidentificationsystems Biochemical tests Gram-staining (alternativemethods) examination Morphologic andmicroscopic ® Trainings and Seminars or quality control

[email protected] Fax +49.551.308.1293 Phone +49.551.308.1227 37079 Goettingen,Germany August-Spindler-Strasse 11 Training Center Sartorius StedimBiotechGmbH EXPAND Dr. DagmarSimons Registration andInformation – – – – Practical Exercises – – – – – – limitations –Sterilitytestmethods|Test –Regulationsandguidance – Sterilitytesting Theoretical Aspects on SterilityTesting Workshop – – – Practical Exercises – – – – – Theoretical Aspects Microbiological QualityControl Use ofLightMicroscopyin Sterility testisolatorsinroutine Observation andinterpretationofresults Performing sterilitytestinisolators devices) (LVPs|SVPs|ampoules|antibiotics|medical Sterility testingofdifferentproducts Sterility testisolators yeast andmolds Microscopic examinationofbacteria, phase contrast field| sample withbrightfield|dark Microscopic examinationofprepared illumination Adjusting themicroscope|Köhler microscopy of bacteriaandfungiusinglight Determination ofthecellularmorphology in microbiologicalQC Fluorescence microscopy|applications contrast field|phase Bright field|dark illumination Optical train|Köhler Basic principlesofmicroscopy Microbiological monitoring H Standards andregulation|Design Validation Interpretation ofsterilitytestresults 2 O 2 ® decontamination TrainingsandSeminars 67

EXPAND® Trainings and Seminars Chemical Compatibility 1. Filter Materials and Mini Cartridges

Cellulose Cellulose Reg. PTFE Poly- Glass Polycar- Poly- Sartobran® P Sartofluor® acetate nitrate Cellulose amide fiber bonate ether- cartridge cartridge sulfone

Solvents 111 113 184 118 250 134 230 154 Acetone – – • • – • – – E Acetonitrile ? ? • • – ? ? • ? ? Gasoline • • • • • • • • V – Benzene • • • • • • ? • – – Benzyl alcohol • • • • ? – • n-Butyl acetate – • • • • • • E ? n-Butanol • • • • • • • • • • Cellosolve • – • • ? • – • – – Chloroform – • • • • • – – – – Cyclohexane • • ? • • – V Cyclohexanone – – • • • • ? ? – – Diethylacetamide – – • • • • ? ? – ? Diethyl ether • – • • • • • ? – – Dimethyl formamide – – • • – ? – • Dimethylsulfoxide – – • • • • – – — • Dioxane – – • • • • – • – • Ethanol, 98% • • • • • • • • • Ethyl acetate – – • • • • ? – – – Ethylene glycol • • • ? • • • • • Formamide ? ? ? • ? • – ? – • Glycerine • • • • • • • • • • n-Heptane • • • • ? • ? ? • V n-Hexane • • • • • • • ? V – Isobutanol • • • • • ? – • Isopropanol • • • • • • • • • Isopropyl acetate – • • ? • ? • – • Methanol, 98% • – • • ? • • • • • Methyl acetate – – • • • • ? – – • Methylene chloride – • • • • – – – – Methyl ethyl ketone – – • • • • ? – – • Methyl isobutyl ketone • – • • • • ? ? – – Monochlorobenzene • • • • • • – ? V V Nitrobenzene • • • • • – ? – – n-Pentane • • • • • • • ? V V Perchloroethylene • • • • • • • ? V V Pyridine – – • • • • – – – – Carbon tetrachloride • • • • • ? • – ? Tetrahydrofuran – – • • • • – – – – Toluene • • • • • • ? • – –

Key to symbols see next page.

68 Cellulose Cellulose Reg. PTFE Poly- Glass Polycar- Poly- Sartobran® P Sartofluor® acetate nitrate Cellulose amide fiber bonate ether- cartridge cartridge sulfone

Solvents 111 113 184 118 250 134 230 154 Trichloroethane • • • ? • ? ? – ? Trichloroethylene • • • • • • – • – ? Xylene • • • • • • • • – –

Acids Acetic acid, 25% • • • • ? • • ? Acetic acid, 96% – – • • – ? ? • – • Hydrofluoric acid, 25% • • – ? • ? – – Hydrofluoric acid, 50% • – • – ? • ? – – Perchloric acid, 25% – • – ? ? ? – • Phosphoric acid, 25% • • – ? ? ? • • Phosphoric acid, 85% • – ? – ? – V/E Nitric acid, 25% – – • – ? • • – V Nitric acid, 65% – – – • – ? • • – – Hydrochloric acid, 25% – – • – ? • • – V/E Hydrochloric acid, 37% – – – • – ? • • – V/E Sulfuric acid, 25% – • – • ? • – • Sulfuric acid, 98% – – – • – ? – ? – – Trichloroacetic acid, 25% – • • – ? ? ? – •

Bases Ammonium, 1N • • • • • – • E • Ammonium hydroxide, 25% – – • – • – • Potassium hydroxide, 32% – – • – • – • Sodium hydroxide, 32% – – • – • – • Sodium, 1N – • • • – • – •

Aqueous solutions Formaline, 30% • • • • • – • Sodium hypochlorite, 5% • • • • ? ? – • Hydrogen peroxide, 35% • • • ? ? ? • •

Key to symbols • = compatible = limited compatibility – = not compatible ? = not tested E = compatible after replacing silicone O-ring with an EPDM O-ring V = compatible after replacing the silicone O-ring with a Viton O-ring

Contact time: 24 hours at 20°C Chemical compatibilities can be influenced by various factors. Therefore, we recommend that you confirm compatibility with the liquid you wish to filter by performing a trial filtration run before you begin with actual filtration.

69 2. Filter Holder, Cartridge Housing and O-ring Materials

Glass Poly- Poly- PTFE Stainless EPDM PTFE Silicone Viton carbonate propylene steel O-ring O-ring O-ring O-ring

Solvents Acetone • • • • • • – – Acetonitrile • ? • • • • – • Gasoline • • • • – • – • Benzene • – – • • – • – • Benzyl alcohol • – • • • • • • n-Butyl acetate • – • • • • – – n-Butanol • • • • • • • • • Cellosolve • – – • • • – – Chloroform • – – • • – • – • Cyclohexane • • • • – • – • Cyclohexanone • – • • • – • – – Diethylacetamide • – ? • • ? • • – Diethyl ether • – • • – • – – Dimethyl formamide • – • • • • • – Dimethylsulfoxide • ? ? • • ? • – Dioxane • – • • • • – – Ethanol, 98% • • • • • • • • • Ethyl acetate • – • • • • • – – Ethylene glycol • • • • • • • • • Formamide • – • • • • • – Glycerine • • • • • • • • n-Heptane • • • • • – • • • n-Hexane • • • • • – • – • Isobutanol • • • • • • • • • Isopropanol • • • • • • • • Isopropyl acetate • • • • • • • – – Methanol, 98% • – • • • • • • • Methyl acetate • ? • • • • • – – Methylene chloride • – – • • – • – Methyl ethyl ketone • – • • • • • – – Methyl isobutyl ketone • – ? • • – • – – Monochlorobenzene • – • • • – • – • Nitrobenzene • – • • – • – – n-Pentane • • • • • – • – • Perchloroethylene • – • • – • – • Pyridine • – • • – • – – Carbon tetrachloride • – • • – • – • Tetrahydrofuran • – • • – • – – Toluene • – • • • – • –

Key to symbols see next page.

70 Glass Poly- Poly- PTFE Stainless EPDM PTFE Silicone Viton carbonate propylene steel O-ring O-ring O-ring O-ring

Solvents Trichloroethane • – ? • • – • – • Trichloroethylene • – – • • – • – • Xylene • – • • – • –

Acids Acetic acid, 25% • • • • • • • • – Acetic acid, 96% • – • • • • • ? – Hydrofluoric acid, 25% – – • • – • – Hydrofluoric acid, 50% – – • • – • – Perchloric acid, 25% • • • – • • – • Phosphoric acid, 25% • • • • • – • Phosphoric acid, 85% • • • • • – • Nitric acid, 25% • – • • – • – • Nitric acid, 65% • – – • – – • – • Hydrochloric acid, 25% • • • – • – • Hydrochloric acid, 37% • – • • – • • – • Sulfuric acid, 25% • • • • • • – • Sulfuric acid, 98% • – – • – – • – • Trichloroacetic acid, 25% • • • – • • – –

Bases Ammonium, 1N • – • • • • • – – Ammonium hydroxide, 25% • – • • • • • • – Potassium hydroxide, 32% • – • • • • • Sodium hydroxide, 32% • – • • • • • • Sodium, 1N • – • • • • • • •

Aqueous solutions Formaline, 30% • • • • • • • • Sodium hypochlorite, 5% • • • • • • • • • Hydrogen peroxide, 35% • • • • • • • • •

Key to symbols • = compatible = limited compatibility – = not compatible ? = not tested

71 3. Ready-to-Connect Filtration Units

Midisart® Minisart® Minisart® Minisart® Minisart® Sartobran® Sartobran® P Sartofluor® Sartolab® 2000 HY RC SRP 300 Capsule Capsule P20

Solvents Acetone • – – • – – – • – Acetonitrile • – ? • • ? ? ? ? Gasoline • • • • • • • • Benzene • – – ? • – – – Benzyl alcohol • ? ? ? • • – n-Butyl acetate • – – ? • • • • – n-Butanol • • • • • • • Cellosolve – – • – – – Chloroform • – – • • – – • – Cyclohexane • – – ? • • Cyclohexanone • – – ? • – – • – Diethylacetamide • – – • • – – • – Diethyl ether • ? ? ? • • – Dimethyl formamide • – – ? • – – • – Dimethylsulfoxide • – – • • – – • – Dioxane • – – • • – – – Ethanol, 98% • – – • • • • • • Ethyl acetate • • • – – – Ethylene glycol • ? ? • • • • • • Formamide • ? ? ? • ? ? • – Glycerine • • • ? • • • • n-Heptane • • • ? • • • • • n-Hexane • • • • • • • • • Isobutanol • • • • Isopropanol • – • • • • Isopropyl acetate • ? • • Methanol, 98% • – – • • • • • – Methyl acetate • – – ? • – – • – Methylene chloride • – – • • – – – Methyl ethyl ketone • – – • • – – • – Methyl isobutyl ketone • ? ? ? • ? ? • – Monochlorobenzene • ? ? ? • • • • – Nitrobenzene • ? ? ? • • – n-Pentane • • • • • • • • • Perchloroethylene • ? • • – Pyridine • – – ? • – – • – Carbon tetrachloride • ? • • – Tetrahydrofuran • – – • • – – – Toluene • – – • • • • • –

Key to symbols see next page.

72 Midisart® Minisart® Minisart® Minisart® Minisart® Sartobran® Sartobran® P Sartofluor® Sartolab® 2000 HY RC SRP 300 Capsule Capsule P20

Solvents Trichloroethane • • • ? ? • – Trichloroethylene ? ? ? – – – – Xylene • – – • • • –

Acids Acetic acid, 25% • ? ? • • • • Acetic acid, 96% • – – ? • – – • – Hydrofluoric acid, 25% • ? • • • • – Hydrofluoric acid, 50% • ? • – – • – Perchloric acid, 25% • ? ? ? • – – • – Phosphoric acid, 25% • • • ? • • • • • Phosphoric acid, 85% – ? ? ? – – Nitric acid, 25% • – – ? • – – • – Nitric acid, 65% • – – ? • – – – Hydrochloric acid, 25% • – – ? • – – • – Hydrochloric acid, 37% • – – ? • – – • – Sulfuric acid, 25% • – – ? • – – • – Sulfuric acid, 98% • – – ? • – – • – Trichloroacetic acid, 25% • – – • • – – • –

Bases Ammonium, 1N • • • ? • • • • – Ammonium hydroxide, 25% • ? • • – Potassium hydroxide, 32% • – – ? • – – • – Sodium hydroxide, 32% • – – ? • – – • – Sodium, 1N • ? • • –

Aqueous solutions Formaline, 30% • – – ? • • Sodium hypochlorite, 5% • • • ? • – – • • Hydrogen peroxide, 35% • • • ? • • • • •

Key to symbols • = compatible = limited compatibility – = not compatible ? = not tested

73 Index

Absorbent Pads 28 Manifolds, multi-branch 47 Accessories for vacuum filter holders MD8 airscan® 6, 8, 10 and manifold systems 50 MD8 calibration unit 10 Airborne bacteria and viruses 5, 10 MD8 devices 10 Air Monitoring 6 Media 24 AirPort MD8 7, 10 Membranes without grid 20 Air Sampler 6, 9 Microsart® Combi.jet 42 Aluminum stack 10 Microsart® e.jet 46, 53, 54 Microsart® e.motion 14, 27 BACTair™ 9 Microsart® e.motion Dispenser 14 Biosart® 100 Monitor 30 Microsart® e.motion Membrane Filters 15, 27 Biosart® 100 Nutrient media 32 Microsart® @filter 100 and 250 33 Biosart® 250 Funnel 37 Microsart® @vance® 33 Microsart® Funnel 100 and 250 35 Calibration unit 10 Microsart® maxi.vac 52 Case for MD8 airscan® 11 Microsart® mini.vac 52 Cellulose acetate 13, 20, 22, 65 Minisart® SRP 41 Cellulose Nitrate (Cellulose Ester) 12, 16, 18, 20, Multi-branch manifolds, traditional 47 22, 57 Chemical Compatibility 68 NPS 4, 24, 55 Colony counter 56 Nutrient media broth 32 Colony counting 4, 12 Nutrient pad sets in petri dishes 24 Combisart® multi-branch systems 38 CONFIDENCE® Validation Services 66 Pedal (foot switch) for Microsart® e.motion 14 Container for anaerobic incubation 57 Peristaltic pump 65 Culture media 4, 24, 28 Preassembled Monitors 5, 30

Dispenser 4, 14, 29 Quick connection for tubings 43, 54 Dosing syringe 55 Ready-to-use units including media 30 EXPAND® Trainings and Seminars 67 Reusable sterility test system 64 Rubber vacuum hose 51 Filter dispenser 14 Filter holders and manifolds 38 Sartochem® 60, 61 Filter holders, individual 47 School kit for microbiological experiments 58 Single-use funnels 5, 37 Gelatine membrane filters 8 Stainless steel filter holder, 50 mm, Gridded membrane filters for colony counting 12 with vacuum control 47 Gridded membrane filters, type 111 21 Stainless steel funnels 5, 38 Gridded membrane filters, type 113 21 Sterility Testing Systems 59 Gridded membrane filters, type 114 17, 19 Sterisart® 5, 59 Gridded membrane filters, type 130 17, 19 Sterisart® NF 60 Gridded membrane filters, type 131 23 Sterisart® Universal Pump 59 Gridded membrane filters, type 135 23 Suction flask, 2 liter capacity 50 Gridded membrane filters, type 138 17, 19 Gridded membrane filters, type 139 17, 19 Vacusart® 51 Gridded membranes 12, 16 Vacuum filtration system 44

Hand-operated vacuum pump with gauge 55 Water jet pump 55 High flow membranes 4, 13 Water trap, Vacusart® 51 Hydrophobic edged membranes 22 Woulff’s bottle 51

Laboratory pump, 90% 55 Laboratory pump, 98% 55 For the purpose of product development we reserve the right to make changes without notice.

Sales and Service Contacts For further contacts, visit www.sartorius-stedim.com

Europe America Asia|Pacific Germany Denmark USA Australia Sartorius Stedim Biotech GmbH Sartorius Stedim Nordic A/S Sartorius Stedim North America Inc. Sartorius Stedim Australia Pty. Ltd. August-Spindler-Strasse 11 Hoerskaetten 6D, 1. 5 Orville Drive Unit 5, 7-11 Rodeo Drive 37079 Goettingen DK-2630 Taastrup Bohemia, NY 11716 Dandenong South Vic 3175 Phone +49.551.308.0 Phone +45.7023.4400 Toll-Free +1.800.368.7178 Phone +61.3.8762.1800 Fax +49.551.308.3289 Fax +45.4630.4030 Fax +1.631.254.4253 Fax +61.3.8762.1828 www.sartorius-stedim.com Hungary Sartorius Stedim SUS Inc. China Sartorius Stedim Hungária Kft 1910 Mark Court Sartorius Stedim Beijing Sartorius Stedim Systems GmbH Kagyló u. 5 Concord, CA 94520 Representative Office Schwarzenberger Weg 73–79 2092 Budakeszi No. 33, Yu’an Road, 34212 Melsungen Phone +1.925.689.6650 Airport Industrial Zone B, Shunyi District Phone +36.23.457.227 Toll Free +1.800.914.6644 Phone +49.5661.71.3400 Beijing 101300 Fax +36.23.457.147 Fax +1.925.689.6988 Fax +49.5661.71.3702 Phone +86.10.80426516 www.sartorius-stedim.com Fax +86.10.80426580 Italy Sartorius Stedim Systems Inc. Sartorius Stedim Italy S.p.A. 201 South Ingram Mill Road Via dell’Antella, 76/A Springfield, MO 65802 Sartorius Stedim Shanghai France 50012 Antella-Bagno a Ripoli (FI) Represantative Office Sartorius Stedim Biotech S.A. Phone +1.417.873.9636 Room 618, Tower 1, German Centre, ZI Les Paluds Phone +39.055.63.40.41 Fax +1.417.873.9275 Shanghai, PRC., 201203 Avenue de Jouques – BP 1051 Fax +39.055.63.40.526 13781 Aubagne Cedex Phone +86.21.28986393 Argentina Fax +86.21.28986392.11 Phone +33.442.845600 Netherlands Sartorius Argentina S.A. Fax +33.442.845619 Sartorius Stedim Netherlands B.V. Int. A. Avalos 4251 Edisonbaan 24 B1605ECS Munro Sartorius Stedim Guangzhou Office 3439 MN Nieuwegein Buenos Aires Room 704, Broadway Plaza, Sartorius Stedim France SAS No. 233–234 Dong Feng West Road ZI Les Paluds Phone +31.30.6025080 Phone +54.11.4721.0505 Guangzhou 510180 Avenue de Jouques – CS 71058 Fax +31.30.6025099 Fax +54.11.4762.2333

13781 Aubagne Cedex Phone +86.20.8351.7921 W

Fax +86.20.8351.7931 | Phone +33.442.845600 Poland Brazil Fax +33.442.846545 Sartorius Stedim Poland Sp. z o.o. Sartorius do Brasil Ltda e . ul. Wrzesinska 70 Av. Dom Pedro I, 241 India 62-025 Kostrzyn Bairro Vila Pires Sartorius Stedim India Pvt. Ltd. chlorin Austria Santo André #69/2-69/3, Jakkasandra Phone +48.61.647.38.40 o f use Sartorius Stedim Austria GmbH Fax +48.61.879.25.04 São Paulo Kunigal Road, Nelamangala Tq Franzosengraben 12 Cep 09110-001 Bangalore – 562 123 any A-1030 Vienna Spain Phone +55.11.4451.6226 Phone +91.80.4350.5361 Phone +43.1.7965763.18 Sartorius Stedim Spain SA Fax +55.11.4451.4369 Fax +91.80.4350.5253 without Fax +43.1.796576344 C/Isabel Colbrand 10,

Oficina 70 Mexico Japan bleached Belgium Polígono Industrial de Fuencarral Sartorius de México S.A. de C.V. Sartorius Stedim Japan K.K. Sartorius Stedim Belgium N.V. 28050 Madrid Circuito Circunvalación Poniente No. 149 KY Building, 8–11 been Leuvensesteenweg, 248/B Phone +34.90.2110935 Ciudad Satélite Kita Shinagawa 1-chome has 1800 Vilvoorde 53100 Naucalpan, Estado de México Shinagawa-ku Fax +34.91.3589623 that Tokyo 140-0001 Phone +32.2.756.06.80 Phone +52.5555.62.1102 YYYY Fax +32.2.756.06.81 Fax +52.5555.62.2942 Phone +81.3.3740.5407 |

Switzerland p aper on Sartorius Stedim Switzerland AG Fax +81.3.3740.5406 MM

Ringstr. 24 a Ver. · 8317 Tagelswangen Malaysia G ermany in Phone +41.52.354.36.36 Sartorius Stedim Malaysia Sdn. Bhd. Fax +41.52.354.36.46 Lot L3-E-3B, Enterprise 4

Technology Park Malaysia Printed

Bukit Jalil 00000-000-00

U.K. No.:

57000 Kuala Lumpur notice. Sartorius Stedim UK Limited Phone +60.3.8996.0622

Longmead Business Park Order Fax +60.3.8996.0755 · Blenheim Road, Epsom without Surrey KT19 9 QQ

Phone +44.1372.737159 Singapore change Fax +44.1372.726171 Sartorius Stedim Singapore Pte. Ltd. to 1 Science Park Road, XX-0000-e07051

The Capricorn, #05-08A, subject

Singapore Science Park 2 N o.: Singapore 117528 Phone +65.6872.3966 Publication Fax +65.6778.2494 Specifications subject to change without notice. Printed in Germany on paper that has been bleached any use of chlorine. | W Specifications Publication No.: SL-1530-e11044 · Order 85034-538-81 Ver. 04 | 2011