RESEARCH ARTICLE BRDT is an essential epigenetic regulator for proper chromatin organization, silencing of sex chromosomes and crossover formation in male meiosis Marcia Manterola1,2, Taylor M. Brown1, Min Young Oh1, Corey Garyn1, Bryan J. Gonzalez3, Debra J. Wolgemuth1,3,4* a1111111111 1 Department of Genetics & Development, Columbia University Medical Center, New York, NY, United States of America, 2 Human Genetics Program, Institute of Biomedical Sciences, Faculty of Medicine, a1111111111 University of Chile, Santiago, Chile, 3 Institute of Human Nutrition, Columbia University Medical Center, New a1111111111 York, NY,United States of America, 4 Department of Obstetrics & Gynecology, Columbia University Medical a1111111111 Center, New York, NY,United States of America a1111111111 *
[email protected] Abstract OPEN ACCESS Citation: Manterola M, Brown TM, Oh MY, Garyn The double bromodomain and extra-terminal domain (BET) proteins are critical epigenetic C, Gonzalez BJ, Wolgemuth DJ (2018) BRDT is an readers that bind to acetylated histones in chromatin and regulate transcriptional activity essential epigenetic regulator for proper chromatin and modulate changes in chromatin structure and organization. The testis-specific BET organization, silencing of sex chromosomes and member, BRDT, is essential for the normal progression of spermatogenesis as mutations in crossover formation in male meiosis. PLoS Genet 14(3): e1007209. https://doi.org/10.1371/journal. the Brdt gene result in complete male sterility. Although BRDT is expressed in both sper- pgen.1007209 matocytes and spermatids, loss of the first bromodomain of BRDT leads to severe defects Editor: P. Jeremy Wang, University of in spermiogenesis without overtly compromising meiosis. In contrast, complete loss of Pennsylvania, UNITED STATES BRDT blocks the progression of spermatocytes into the first meiotic division, resulting in a Received: April 25, 2017 complete absence of post-meiotic cells.