Establishment of the Fungal Entomopathogen Beauveria Bassiana (Ascomycota: Hypocreales) As an Endophyte in Cocoa Seedlings (Theobroma Cacao)
Mycologia, 97(6), 2005, pp. 1195–1200. # 2005 by The Mycological Society of America Lawrence, KS 66044-8897 Establishment of the fungal entomopathogen Beauveria bassiana (Ascomycota: Hypocreales) as an endophyte in cocoa seedlings (Theobroma cacao) Francisco Posada endophyte in woody plant hosts, can spread within Fernando E. Vega1 host tissues and can protect host plants against Insect Biocontrol Laboratory, U.S. Department of herbivores, such as the cocoa pod borer, has not Agriculture, Agricultural Research Service, Bldg. 011A, been evaluated. Our ultimate goal is to determine BARC-W, Beltsville, Maryland 20705 whether cocoa plants inoculated with B. bassiana in the seedling stage can sustain the fungus in the field, and more importantly, whether B. bassiana can be Abstract: The fungal entomopathogen Beauveria detected in the pod, where it ideally would help bassiana became established as an endophyte in in control the cocoa pod borer. vitro-grown cocoa seedlings tested for up to 2 mo The cocoa pod borer is believed to be endemic to after inoculation to the radicle with B. bassiana Southeast Asia and to have shifted from rambutan suspensions. The fungus was recovered in culture (Nephelium lappaceum L.), pulasan (N. mutabile from stems, leaves and roots. B. bassiana also was Blume) and nam-nam (Cynometra cauliflora L.) to detected as an epiphyte 1 and 2 mo postinoculation. cocoa, which was introduced from the American Penicillium oxalicum and five bacterial morphospecies continent in the 16th century (Malaysian Plant also were detected, indicating that these were present Protection Society 1987). as endophytes in the seed. Female cocoa pod borers can lay up to 100 eggs on Key words: biological control, cacao, cocoa pod the surface of the cocoa pod.
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