mLRP-10 VersaClone cDNA Catalog Number: RDC1306

Specifications: Description This shuttle vector contains the complete ORF for the gene of interest, Gene: mLrp10 along with a Kozak consensus sequence for optimal translation initiation. It is inserted NotI to AscI. The gene insert is flanked with Accession: BAB20775 convenient multiple cloning sites which can be used to easily cut and transfer the gene cassette into your desired expression vector. Insert size: 2155bp Preparation and Storage Concentration: 10µg at 0.2μg/μL Formulation cDNA is provided in 10 mM Tris-Cl, pH 8.5

Shipping Ships at ambient temperature

Stability 1 year from date of receipt when stored at -20°C to -80°C Storage Use a manual defrost freezer and avoid repeated mLRP-10 cDNA freeze-thaw cycles.

Plasmid

Lrp10 low-density lipoprotein receptor-related protein 10 ZraI BmgBI AatII BstAPI HpaI [ Mus musculus (house mouse) ] SspI NdeI HindIII XmnI BamHI EcoRV Also known as: Lrp9 ScaI BmtI StuI NheI NotI EcoNI EagI Summary: BtgI NcoI LRP10 is a member of the low AMP BsmI density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor- RDC1306 mediated endocytosis of lipoprotein 4889 bps BsaAI SnaBI and protein ligands. LRP10 is highly Tth111I mLRP10 (1-713) expressed in heart, lung, and liver. COLE1 AvrII LRP10 may be involved in the uptake of lipoprotein APOE in liver. NaeI NgoMIV

BtgZI XhoI PciI AarI PspXI SapI BbsI XmaI BglII SmaI ApaI SalI PspOMI XbaI PpuMI Bst1107I Van91I BssHII AscI

FOR RESEARCH USE ONLY

NOT FOR USE IN HUMANS

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> RDC1306 Plasmid DNA Sequence 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 101 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 201 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 401 ggagacgtgt taacaagctt ggatccgata tcgctagcgc ggccgccacc atgctatcgg ccctccctct tcttttcctc ctcctcggag gtgctctggc 501 ccgcccagac cggatcactt tcccacgttc tgcttgtgag gcgcccccag cagtgctctc agaagtacaa ggcactttac agaggcctct aggccgggac 601 agccgaagct cccctgccaa ctgcacctgg gttatcctgg gcagcaagga tcagacagta actgtcaggt tccagaagct gcatctggcc tgtggctcag 701 agcatttaat cctgcactcc cctctacagc caccaatctc cctgtgtgag gccccttctg gccctttgca gctaccaggg ggcaatgtca ccattacata 801 cagctatgct ggggccagag cacccatggg ccagggcttc ttgctgactt acagtcaaga ttggctactg tgcctgcaag aagaattcca gtgcctgaac 901 caccgctgca ttccagctgc tcagcgctgt gatgggattg atgcctgcgg ggatggctca gacgaggcag gctgcagctc agatccattc cctaacctga 1001 acccagcccc cgcaccaact ctggcctgca atctcacctt ggaggacttt tatggggtct tttcttcccc tggatattca cacctggcct cagtctccca 1101 cccccagtcc tgcctgtggc tgctggaccc ccatgatggc cggaggctgg cagtgcgctt cacagccctg gacttgagtt acggagatgc agtgcatgtg 1201 tatgatggtg ctggaccccc cgagacccct cgactgctac gtagcctcac ccacttcagc aatggcaagg ctgtcactgt ggagaccctg tctggtcagg 1301 ctgttgtgtc ctaccacaca gttgcttgga gcagtggccg gggctttaat gctacctacc atgtccgggg ctactgttta ccttgggaca gaccctgtgg 1401 cttgggctct ggcctggggg ctagtgagaa cctaggtgag cgttgctata gcgaggcaca gcgctgtgat ggctcatggg actgtgccga tggcacagat 1501 gaggagggtt gccctggctg cccaccaggg cacttcccct gtggagctgc aggcacccct ggtgccacag cctgctacct gcctgctgac cgctgcaact 1601 accagacgtt ctgcgccgat ggagcggatg agaggcgctg ccggcattgc cagcccggca acttccggtg ccgggatgag aagtgtgtgt atgagacatg 1701 ggtgtgtgat gggcagccag actgtactga cggcagtgat gagtgggact gctcctacgc cctgccccga aaagtcatca cagcagcagt cattggcagc 1801 ctggtgtgtg gcctgttgct ggtcatcgct ctcggctgca cctgcaaact ctatgccatc cgcacccagg aatacagcat ctttgccccg ctctcccgga 1901 tggaggctga gattgtgcag caacaggcac ccccttccta tgggcagctc attgcccagg gtgccatccc gcctgtggaa gacttcccca cagagaaccc 2001 taacgataac tctgtgctgg gaaacctacg ttctctgctt cagatcttac gccaggatat gactccaggt ggcacttcag ggggccgccg tcgccagcgt 2101 ggacgctccg tccgccgtct ggttcgccgt ctccgtcgtt ggggcctgct tcctcgaact aataccccag ctcgggcccc tgaaaccaga tcccaggtca 2201 caccctctgt tccctctgag gccctggatg acagcacagg ccatgcctgt gagggtgggg cagtaggggg gcaagatggg gagcaggctc ctccactgcc 2301 catcaagacc cccatcccaa ccccaagcac acttcctgcc cttgctactg tctctgagac cccagggcca ctaccctcag tgcctgtaga atcatcactg 2401 ttgtctggag ttgtccaggt cctacgaggc cgcctcctac ccagcctgtg gtccccaggc cccacttgga cccaaactgg aactcacaca acagtcctgt 2501 ccccagagga tgaggacgat gtattgttat tgccactggc tgagccagaa gtctgggtgg tggaggcaga ggatgaacca ttgcttgcct aaaggcgcgc 2601 cagtatactc tagagtcgac acccggggaa ttcctcgagc gctcgtctct agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 2701 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 2801 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 2901 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3001 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 3101 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 3301 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 3401 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3501 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3601 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 3701 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 3801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 3901 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 4001 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4201 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 4301 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 4401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4501 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 4601 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 4701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 4801 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc

> RDC1306 Translated Insert Sequence 1 mlsalpllfl llggalarpd ritfprsace appavlsevq gtlqrplgrd srsspanctw vilgskdqtv tvrfqklhla cgsehlilhs plqppislce 101 apsgplqlpg gnvtitysya garapmgqgf lltysqdwll clqeefqcln hrcipaaqrc dgidacgdgs deagcssdpf pnlnpapapt lacnltledf 201 ygvfsspgys hlasvshpqs clwlldphdg rrlavrftal dlsygdavhv ydgagppetp rllrslthfs ngkavtvetl sgqavvsyht vawssgrgfn 301 atyhvrgycl pwdrpcglgs glgasenlge rcyseaqrcd gswdcadgtd eegcpgcppg hfpcgaagtp gatacylpad rcnyqtfcad gaderrcrhc 401 qpgnfrcrde kcvyetwvcd gqpdctdgsd ewdcsyalpr kvitaavigs lvcglllvia lgctcklyai rtqeysifap lsrmeaeivq qqappsygql 501 iaqgaippve dfptenpndn svlgnlrsll qilrqdmtpg gtsggrrrqr grsvrrlvrr lrrwgllprt ntparapetr sqvtpsvpse alddstghac 601 eggavggqdg eqapplpikt piptpstlpa latvsetpgp lpsvpvessl lsgvvqvlrg rllpslwspg ptwtqtgtht tvlspededd vlllplaepe 701 vwvveaedep lla

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