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MRPL28 Antibody Cat MRPL28 Antibody Cat. No.: 19-930 MRPL28 Antibody Immunohistochemistry of paraffin- embedded rat ovary using MRPL28 Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (19-930) at dilution of antibody (19-930) at dilution of 1:100 (40x lens). 1:100 (40x lens). Immunohistochemistry of paraffin- embedded human gastric cancer Immunofluorescence analysis of U2OS cells using MRPL28 antibody (19-930). using MRPL28 antibody (19-930) at Blue: DAPI for nuclear staining. dilution of 1:100 (40x lens). September 24, 2021 1 https://www.prosci-inc.com/mrpl28-antibody-19-930.html Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MRPL28 antibody (19-930). Western blot was performed from the immunoprecipitate using MRPL28 antibody (19-930) at a dilition of 1:1000. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of IMMUNOGEN: human MRPL28 (NP_006419.2). TESTED APPLICATIONS: IF, IHC, IP, WB WB: ,1:500 - 1:2000 IHC: ,1:50 - 1:200 APPLICATIONS: IF: ,1:50 - 1:100 IP: ,1:50 - 1:200 POSITIVE CONTROL: 1) MCF7 2) HepG2 3) 293T 4) A-549 PREDICTED MOLECULAR Observed: 30kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG September 24, 2021 2 https://www.prosci-inc.com/mrpl28-antibody-19-930.html CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: MRPL28 39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, ALTERNATE NAMES: Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1 GENE ID: 10573 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic BACKGROUND: ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 3 https://www.prosci-inc.com/mrpl28-antibody-19-930.html.
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