MRPL28 Antibody Cat. No.: 19-930

MRPL28 Antibody

Immunohistochemistry of paraffin- embedded rat ovary using MRPL28 Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (19-930) at dilution of antibody (19-930) at dilution of 1:100 (40x lens). 1:100 (40x lens).

Immunohistochemistry of paraffin- embedded human gastric cancer Immunofluorescence analysis of U2OS cells using MRPL28 antibody (19-930). using MRPL28 antibody (19-930) at Blue: DAPI for nuclear staining. dilution of 1:100 (40x lens).

September 24, 2021 1 https://www.prosci-inc.com/mrpl28-antibody-19-930.html Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug MRPL28 antibody (19-930). Western blot was performed from the immunoprecipitate using MRPL28 antibody (19-930) at a dilition of 1:1000.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Rat

Recombinant fusion containing a sequence corresponding to amino acids 1-256 of IMMUNOGEN: human MRPL28 (NP_006419.2).

TESTED APPLICATIONS: IF, IHC, IP, WB

WB: ,1:500 - 1:2000

IHC: ,1:50 - 1:200 APPLICATIONS: IF: ,1:50 - 1:100

IP: ,1:50 - 1:200

POSITIVE CONTROL: 1) MCF7

2) HepG2

3) 293T

4) A-549

PREDICTED MOLECULAR Observed: 30kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

September 24, 2021 2 https://www.prosci-inc.com/mrpl28-antibody-19-930.html CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: MRPL28

39S L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, ALTERNATE NAMES: Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1

GENE ID: 10573

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

Mammalian mitochondrial ribosomal are encoded by nuclear and help in protein synthesis within the . Mitochondrial (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic BACKGROUND: ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by . This encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize in an HLA-A24-restricted fashion.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 24, 2021 3 https://www.prosci-inc.com/mrpl28-antibody-19-930.html