Research Article MORPHOLOGICAL VARIABILITY in the COMMON SEDGE PLANTS in INDIA
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International Journal of Agriculture Sciences ISSN: 0975-3710&E-ISSN: 0975-9107, Volume 8, Issue 55, 2016, pp.-3000-3007. Available online at http://www.bioinfopublication.org/jouarchive.php?opt=&jouid=BPJ0000217 Research Article MORPHOLOGICAL VARIABILITY IN THE COMMON SEDGE PLANTS IN INDIA TANTWAI KEERTI1*, TIWARI SHARAD1 AND ANSARI TABASSUM2 1Biotechnology Centre, Jawaharlal Nehru Agricultural University, Krishinagar, Adhartal, Jabalpur, 482004, Madhya Pradesh, India 2Department of Botany, NES Science and Commerce College, Jabalpur 482002, India *Corresponding Author: [email protected] Received: September 29, 2016; Revised: November 01, 2016; Accepted: November 02, 2016; Published: November 12, 2016 Abstract- Cyperus is the second largest genus of the sedges or Cyperaceae family, the plants of which are identified as one of the most common agricultural weeds. A total of eighty two sedge plants were collected from twenty one different places covering seven states of India. The plant species and morphological variations among different species were determined based on overall plant growth characteristics. The samples were identified as belonging to seventeen different species of Cyperus and related genera on the basis of UPGMA cluster analysis using Jaccard and Simple Matching coefficients. The mantel test coefficient between these two similarity coefficients was 0.97169. Based on morphological variations, plant samples were identified to be belonging to twelve different species of Cyperus, three species from genus Fimbristylis and two species from genus Kyllinga. Several morphological traits were assessed for identification of plants up to species level, among those spikelet was found the best to be used for the identification of sedge species. Keywords- Sedge, Cyperaceae, Morphological variation, Cluster analysis Citation: Tantwai Keerti, et al., (2016) Morphological Variability in the Common Sedge Plants in India. International Journal of Agriculture Sciences, ISSN: 0975-3710 & E- ISSN: 0975-9107, Volume 8, Issue 55, pp.-3000-3007. Copyright: Copyright©2016 Tantwai Keerti, et al., This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. Academic Editor / Reviewer: Dr Bishun Deo Prasad Introduction disorder etc. Anti-bacterial effect is also found in rhizome extract of C. rotundus Weed populations exhibit a vast range of biodiversity resulting from taxonomic [3]. Antimalarial, anticancer, antimicrobial activities in essential oil from the aerial diversity among them including diversity in particular weedy traits that influences parts of Cyperus kyllingia has also been reported [4]. Cyperus involucratus is their survival, mortality, and reproduction [1]. The ecological diversity of sedges is planted as an ornamental plant. Cyperus iria is used as fodder and its stem is tremendous; with species occurring in almost all habitats. Sedges mostly prefer woven into mats. Leaf and tuber parts of C. iria are used as Tonic, stimulant, moist and sunny sites. Sedges are monocotyledonous flowering plants belonging stomachic and astringent [5]. Kyllinga odorata is known for diaphoretic and diuretic to family Cyperaceae which is commonly known as sedge family. Sedge plants properties [6]. Kyllinga brevifolia is a Paraguayan folk medicine and used as are mostly grass-like herbs, which are abundantly found in moist and damp areas sedative and tonic for nervous system [7]. Fimbristylis miliacea has been found of throughout the world. Cyperus having about 650 species, is the second largest an important role in phytoremediation to absorb heavy metals and zinc in waste genus after Carex in the Cyperaceae family. Members of the Cyperaceae (sedge water treatment [8]. family) resemble the Gramineae (grass family) but are distinguished by three- Identification of the plant species of a potential use is not always easy in the ranked leaves with one-third phyllotaxy and leaves that have closed leaf sheaths, absence of knowledge of the most preferred morphological trait to look upon on usually solid stems, absence of a ligule and each flower subtended by a single site of collection. It is also not feasible to apply costly and sophisticated molecular glume or scale [2]. The morphology of plants belonging to the genus Cyperus techniques to identify plants, which are otherwise regarded as weeds only. Their reported so far is relatively consistent with uniform embryo type. Morphologically correct identification not only helps to formulate effective control strategy but to all the Cyperus species have common characteristics of leaves that are shiny, light explore their potential uses too. Morphological parameters are commonly used as to dark green, three-ranked and corrugated in cross section. Leaf initiation tools for investigating genetic relatedness and diversity among plant populations. terminates with the formation of seed bearing culm. The culm that grows through It is pertinent to study variations in morphological traits among species to the center of the leaf fascicle is erect, simple, smooth and triangular in cross determine how plant genotype and diverse environmental conditions could section. The culm supports a terminal inflorescence, which is a simple, or slightly influence the plant morphology of sedges under uniform conditions. We are compound loose umbel subtended by two or four leaf-like bracts [2]. reporting in the present study, the qualitative genetic--variations in morphological The Cyperus species are herbaceous plants and mostly used as fodder. In and phonological traits in sedges and the identification of sedge plants of particular, C. rotundus and C. esculentus are used as medicinal herb. They are Cyperaceae family up to the species level in plant samples collected at several also called as purple nut sedge and yellow nut sedge, respectively. They are locations from seven states of India. This study was conducted to document commonly found as a perennial weed with slender, scaly creeping rhizomes, morphological characteristics of most common sedge plant species of Cyperus bulbous at the base which is about 1-3 cm. long. They are useful for bowel and related genera from different localities in India. disorder and inflammatory diseases as well as uterine relaxation in both pregnant and non-pregnant women and relieving pain. C. rotundus is a traditional herbal Materials and Methods medicine used widely as antimalarial, analgesic, sedative, and treating stomach A total of eighty two sedge plant samples were collected from several places International Journal of Agriculture Sciences ISSN: 0975-3710&E-ISSN: 0975-9107, Volume 8, Issue 55, 2016 || Bioinfo Publications || 3000 Morphological Variability in the Common Sedge Plants in India located in seven states of India namely Chhattisgarh (CG), Gujarat (GJ), Madhya dendrogram was generated using NTSYSpc version 2.02e software program [9]. Pradesh (MP), Rajasthan (RJ), Tamil Nadu (TN), Uttarakhand (UK) and Uttar The data analysis was done using two different similarity coefficients, Jaccard [10] Pradesh (UP) in the year 2015 [Table-1]. Plant samples were named according to and Simple Matching [11]. Routine procedures of NTSYSpc program (like their places of collection. The morphological observations were recorded at SIMQUAL, SAHN, TREE) in appropriate modules were followed to test the various stages of growth at the site of collection as well as from some of the clustering analysis based on these two similarity coefficients. For the comparison collected plants grown in pots till flowering that occurs in rainy season in India of original matrices generated by implementing Jaccard (J) and Simple Matching (July-October). Observations were recorded for each plant sample on various (SM) similarity coefficients, Mantel test [12] was applied in the option of MXCOMP morphological traits including root, rhizome, nut, stem or culm, leaf, inflorescence, in NTSYSpc program. The plant samples clustered in a group were identified up to spike, spikelet, floret, glume etc. For each trait, different observations were species level by comparing the recorded morphological traits with various tabulated in Microsoft excel sheet and a qualitative data was generated by documented texts such as ‘Flora of Jabalpur’ [13], ‘Flora of British India’ [14], recording 0 and 1 for the absence and presence of the trait, respectively, in a ‘Flora of India’ [15], ‘Flora of Gorakhpurensis’ [16], ‘Hand Book on Weed particular plant sample. This data was subjected to Unweighted Pair Group Identification’ [17] including ‘eFloras’ [18]. Method with Arithmetic Averages (UPGMA) based cluster analysis and Table-1 Eighty two plant samples collected from different locations and their identification (All are annual to perennial herb, Statu s: Common, Least Concern in IUCN Red List) S.N. Place and Sample ID Latitude_Longitude Collection date Species Identified Vernacular Name 1 Chabi-2-MP "22.825720 N 80.700650 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 2 Chennai-2-TN "12.990738 N 80.185958 E" 20-Nov-2015 Cyperus compressus Linn. Mothi 3 Jabalpur-1-MP "23.2203 N 79.9638 E" 15-July-2015 Cyperus compressus Linn. Mothi 4 Jabalpur-2-MP "23.2095 N 79.9533 E" 15-July-2015 Cyperus compressus Linn. Mothi 5 Kundam-2-MP “22.845746 N 81.075466 E” 26-Aug-2015 Cyperus compressus Linn. Mothi 6 Mandla-1-MP "22.611542 N 80.372824 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 7 Mandla-3-MP "22.601269 N 80.378423 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 8 Niwas-2-MP "23.035570 N 80.437791 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 9 Amarkantak-2-MP "22.677726 N 81.758913 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 10 Amarkantak-4-MP "22.682933 N 81.748302 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 11 Dindori-1-MP "22.938539 N 81.080024 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 12 Kapildhara-1-MP "22.700877 N 81.705569 E" 27-Aug-2015 Cyperus compressus Linn. Mothi 13 Sagartola-1-MP “22.941793 N 81.076845 E” 27-Aug-2015 Cyperus compressus Linn. Mothi 14 Garjiya-1-UK “29.462150 N 79.153404 E” 30-Sep-2015 Cyperus cyperoides Linn.