<<

Supporting Information

Article title: Increasing phylogenetic stochasticity at high elevations on summits

across a remote North American wilderness

Authors: Hannah E. Marx, Melissa Richards, Grahm M. Johnson, and David C. Tank

Article acceptance date:

The following Supporting Information is available for this article:

Appendix S1: Table with target-specific primer pair sequences and citations.

Appendix S2: Voucher numbers and collection information for each accession.

Appendix S3: Accession number for the high-throughput (miseq) or GenBank (ncbi) se-

quence used for each gene region and community matrix.

Appendix S4: Table summarizing high-throughput sequencing read statistics and aligned sequence length for each gene region.

Appendix S5: Maximum likelihood phylograms estimated from concatenated gene regions of each dataset (total combined, GenBank, and high-throughput).

Appendix S6: Comparison of phylogenetic community structure between microhabitats within each summit.

Appendix S7: Table summarizing phylogenetic α-diversity.

Appendix S8: Table summarizing phylogenetic β-diversity.

Appendix S9: Comparison of the datasets used to infer phylogenetic relationships among alpine plant species.

1 Table Appendix 1: Table with target-specific primer pair sequences and citations for each gene region (and segments).

Primer 5’ Primer 5’ Sequence 5’ Reference 3’ Primer 3’ Sequence 3’ Reference Pair Name White et al. (1990); White et al. (1990); ITS ITS-5 ggAAggAgAAgTCgTAACAAgg ITS-4 TCCTCCgCTTATTgATATgC Baldwin (1992) Baldwin (1992)

atpB1 S1494R TCAgTACACAAAgATTTAAggTCAT Hoot et al. (1995) S611 AACgTACTCgTgAAggAAATgATCT Hoot et al. (1995) atpB2 S766R TAACATCTCggAAATATTCCgCCAT Hoot et al. (1995) S2 TATgAgAATCAATCCTACTACTTCT Hoot et al. (1995)

ndhF Olmstead and Sweere ndhF Olmstead and Sweere ndhF1 ATggAACAgACATATCAATATgSgTgg gAAAAATTCCCgCCgCTACCATAg 5’-PCR (1994) 803R (1994) Olmstead and Sweere ndhF Olmstead and Sweere ndhF2 ndhF 803 CTATggTAgCggCgggAATTTTTC CgAAACATATAAAATgCRgTTAATCC (1994) 1318R (1994) Olmstead and Sweere ndhF Olmstead and Sweere ndhF3 ndhF 1318 ggATTAACYgCATTTTATATgTTTCg CCCYASATATTTgATACCTTCKCC (1994) 3’-PCR (1994)

matK1 matK1F ACTgTATCgCACTATgTATCA Sang et al. (1997) matK4R gCATCTTTTACCCARTAgCgAAg Bremer et al. (2002) matK2 matk3F AAgATgCCTCTTCTTTGCAT Sang et al. (1997) matK3R gATCCgCTgTgATAATgAgA Sang et al. (1997)

rbcL Olmstead and Palmer rbcL Olmstead and Palmer rbcL ggCCgTCgACATgTCACCACAAACAgARACTAAAgC gATTTCgCCTgTTTCggCTTgTgCTTTATAAA 5’-PCR (1994) Z674R (1994)

a b trn ab CATTACAAATgCgATgCTCT Taberlet et al. (1991) TCTACCgATTTCgCCATATC Taberlet et al. (1991) (B48557) (A49291) c trn cf CgAAATCggTAgACgCTACg Taberlet et al. (1991) f (A50272) ATTTgAACTggTgACACgAg Taberlet et al. (1991) (B49317) 2

*

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chloroplast DNA. Plant Molecular Biology 17:1105–1109.

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for phylogenetics. In PCR Protocols: A Guide to Methods and Applications (M. Innis, D. Gelfand, J. Shinsky, and

T. White, eds.), pages 315–322, Academic Press. Table Appendix S2: Voucher numbers and collection information for each accession.

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: : Sawtooth Mountains: Abies lasiocarpa 14029 Clevenger 2012-022 August 1, 2012 : Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack : Sawtooth National Forest: Sawtooth Mountians: Abies lasiocarpa 14351 Marx 2013-120 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Sawtooth Mountains: Abies lasiocarpa 14232 Zion 2012-034 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Achillea millefolium var alpicola 14287 Marx 2013-193 July 25, 2013 44.04019 -114.58878 : summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: 4 Achillea millefolium var alpicola 14431 Marx 2013-228 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Achillea millefolium var alpicola 14619 Marx 2013-318 August 9, 2013 43.74938 -114.1312 : south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Achillea millefolium var alpicola 14665 Marx 2013-366 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Achillea millefolium var alpicola 14136 Zion 2012-014 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Aconogonon phytolaccaefolium 14229 Zion 2012-031 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountains: Agoseris aurantiaca 14571 Marx 2013-270 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Agrostis variabilis NA Marx 2013-220 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Androsace septentrionalis 14464 Marx 2013-263 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Androsace septentrionalis NA Marx 2013-334 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Androsace septentrionalis 14689 Marx 2013-392 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Anemone drummondii 14309 Marx 2013-078 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Anemone drummondii 14682 Marx 2013-385 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Anemone drummondii var drummondii 13562 Marx 2012-029 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria alpina 14019 Clevenger 2012-012 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria alpina 14311 Marx 2013-080 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria alpina 14322 Marx 2013-091 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Antennaria alpina 14360 Marx 2013-131 July 22, 2013 43.93825 -114.97142 5 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Antennaria alpina 14258 Marx 2013-164 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Antennaria alpina 14591 Marx 2013-290 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Antennaria alpina 14661 Marx 2013-362 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Antennaria alpina 14684 Marx 2013-387 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Antennaria corymbosa 14418 Marx 2013-212 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria lanata 14016 Clevenger 2012-007 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria lanata 14312 Marx 2013-081 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria microphylla 14004 Marx 2012-051 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Antennaria microphylla 14348 Marx 2013-117 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Antennaria rosea 14273 Marx 2013-179 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria umbrinella 13540 Marx 2012-005 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Antennaria umbrinella 14446 Marx 2013-244 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Antennaria umbrinella 14460 Marx 2013-258 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Antennaria umbrinella 14601 Marx 2013-300 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Antennaria umbrinella 14223 Zion 2012-025 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountians: Aquilegia flavescens 14428 Marx 2013-224 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit

6 Idaho: Sawtooth National Forest: Sawtooth Mountains: Arabis cf lemmonii 13546 Marx 2012-011 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountians: Arabis cf lemmonii 14345 Marx 2013-114 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Arabis cf lemmonii 14266 Marx 2013-172 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Arabis cf lemmonii NA Marx 2013-195 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Arabis cf lemmonii 14454 Marx 2013-252 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Arabis cf lemmonii 14630 Marx 2013-329 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Arabis cf lemmonii 14668 Marx 2013-370 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Arabis cf lemmonii 14683 Marx 2013-386 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arabis microphylla 14011 Clevenger 2012-002 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Arabis microphylla NA Marx 2013-064 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arabis microphylla 14306 Marx 2013-075 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Arabis microphylla NA Marx 2013-196 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Arnica chamissonis 14416 Marx 2013-210 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Arnica chamissonis 14582 Marx 2013-281 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arnica latifolia 14303 Marx 2013-072 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arnica latifolia 14316 Marx 2013-085 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

7 Idaho: Sawtooth National Forest: Sawtooth Mountians: Arnica latifolia 14361 Marx 2013-132 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: Pioneer Mountains: Arnica latifolia 14599 Marx 2013-298 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Arnica latifolia NA Marx 2013-417 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arnica latifolia var gracilis 13577 Marx 2012-044 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Arnica latifolia var gracilis 14128 Zion 2012-006 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountians: Arnica mollis 14453 Marx 2013-251 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Arnica parryi 14240 Marx 2013-145 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Artemisia dracunculus 14284 Marx 2013-190 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Aster alpinus 14609 Marx 2013-308 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Aster foliaceus var apricus NA Marx 2013-226 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Aster foliaceus var apricus 14430 Marx 2013-227 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Aster foliaceus var apricus 14583 Marx 2013-282 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Aster foliaceus var apricus 14648 Marx 2013-349 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Aster occidentalis var occidentalis 14420 Marx 2013-214 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Astragalus alpinus 14427 Marx 2013-223 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Astragalus cf eucosmus 14598 Marx 2013-297 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Astragalus kentrophyta 14456 Marx 2013-254 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit

8 Idaho: Sawtooth National Forest: Pioneer Mountains: Astragalus kentrophyta 14620 Marx 2013-319 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Astragalus kentrophyta 14701 Marx 2013-404 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Astragalus kentrophyta 15011 Marx 2013-422 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Bistorta bistortoides 14417 Marx 2013-211 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Bistorta bistortoides 14432 Marx 2013-229 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Bistorta bistortoides 14584 Marx 2013-283 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Bupleurum americanum 13560 Marx 2012-027 July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountians: Bupleurum americanum 14364 Marx 2013-137 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: Sawtooth Mountains: Calamagrostis purpurescens 13551 Marx 2012-016 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Calamagrostis purpurescens 14319 Marx 2013-088 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Calamagrostis purpurescens 14641 Marx 2013-341 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Carex cf luzulina 14458 Marx 2013-256 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountains: Carex cf microptera 14638 Marx 2013-338 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex cf scopulorum 14018 Clevenger 2012-009 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex nardina 14026 Clevenger 2012-019 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack

9 Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex nardina 13549 Marx 2012-015 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Carex nardina 14603 Marx 2013-302 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Carex nardina 14709 Marx 2013-412 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex pachycarpa 14326 Marx 2013-095 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex proposita NA Marx 2013-124 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Carex sp 15003 Marx 2012-013 REXTRACTION 44.14139 -115.01 Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex straminiformis NA Clevenger 2012-010 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex straminiformis 13548 Marx 2012-013 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex straminiformis 13563 Marx 2012-030 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Carex straminiformis 14468 Marx 2013-267 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Carex straminiformis 14635 Marx 2013-335 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Carex straminiformis 14659 Marx 2013-360 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Carex straminiformis 14226 Zion 2012-028 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Cassiope mertensiana var gracilis 14022 Clevenger 2012-015 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Cassiope mertensiana var gracilis NA Marx 2012-021b July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Cassiope mertensiana var gracilis 14292 Marx 2013-060 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

10 Idaho: Sawtooth National Forest: White Cloud Mountains: Cassiope mertensiana var gracilis 14646 Marx 2013-347 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Castelleja applegatei var viscida 13556 Marx 2012-022 July 31, 2012 Thompson Peak: South facing gravel slope just above camp 44.14639 -115.00528 at Profile Lake Idaho: Sawtooth National Forest: Sawtooth Mountains: Castelleja applegatei var viscida 13557 Marx 2012-023 July 31, 2012 Thompson Peak: South facing gravel slope just above camp 44.14639 -115.00528 at Profile Lake Idaho: Sawtooth National Forest: Sawtooth Mountains: Castelleja applegatei var viscida 13558 Marx 2012-024 July 31, 2012 Thompson Peak: South facing gravel slope just above camp 44.14639 -115.00528 at Profile Lake Idaho: Sawtooth National Forest: White Cloud Mountains: Castilleja applegatei 14237 Marx 2013-142 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Castilleja applegatei 14644 Marx 2013-344 August 14, 2013 44.11113 -114.64097 D.O. Lee Peak: along trail Idaho: Sawtooth National Forest: White Cloud Mountains: Castilleja applegatei NA Marx 2013-345 August 14, 2013 44.11113 -114.64097 D.O. Lee Peak: along trail Idaho: Sawtooth National Forest: Sawtooth Mountains: Castilleja cusickii 14296 Marx 2013-065 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Castilleja cusickii 14331 Marx 2013-100 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected along trail Idaho: Sawtooth National Forest: White Cloud Mountains: Castilleja cusickii 14238 Marx 2013-143 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Castilleja cusickii 14368 Marx 2013-198 August 7, 2013 43.76392 -114.16531 Salzburger Spitzl: Meadow below treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Castilleja miniata 14289 Marx 2013-057 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Castilleja miniata 14332 Marx 2013-101 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected along trail Idaho: Sawtooth National Forest: Pioneer Mountians: Castilleja miniata 14369 Marx 2013-199a August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Castilleja miniata 14586 Marx 2013-285 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Castilleja rhexiifolia 14406 Marx 2013-199b August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline 11 Idaho: Sawtooth National Forest: Pioneer Mountains: Cerastium arvense 14575 Marx 2013-274 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Cerastium beeringianum 14451 Marx 2013-249 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Cerastium beeringianum 14628 Marx 2013-327 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Cerastium beeringianum 14632 Marx 2013-331 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Cerastium beeringianum 14687 Marx 2013-390 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Chaenactis alpina 13553 Marx 2012-018 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Chaenactis alpina 13579 Marx 2012-046 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Chaenactis alpina 14297 Marx 2013-066 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Chaenactis alpina 14337 Marx 2013-106 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Chaenactis alpina 14283 Marx 2013-189 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Chaenactis alpina 14433 Marx 2013-230 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Chionophila tweedyi 14013 Clevenger 2012-004 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Chionophila tweedyi 14008 Marx 2012-055 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Chionophila tweedyi 14294 Marx 2013-062 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Cirsium scariosum 14435 Marx 2013-232 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Cirsium subniveum 14288 Marx 2013-194 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline 12 Idaho: Sawtooth National Forest: Pioneer Mountians: Cistanthe umbellata 14434 Marx 2013-231 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Claytonia megarhiza 14293 Marx 2013-061 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Claytonia megarhiza 14341 Marx 2013-110 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Claytonia megarhiza 14267 Marx 2013-173 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Claytonia megarhiza 14448 Marx 2013-246 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Claytonia megarhiza 14614 Marx 2013-313 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Claytonia megarhiza var bellidifolia 14034 Clevenger 2012-027 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Claytonia megarhiza var bellidifolia 14001 Marx 2012-048 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Collomia debilis 14336 Marx 2013-105 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Collomia debilis 14680 Marx 2013-382 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Crepis nana 14699 Marx 2013-402 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Cryptogramma crispa 14298 Marx 2013-067 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountians: Cryptogramma crispa 14333 Marx 2013-102 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Pioneer Mountians: Cryptogramma crispa 14429 Marx 2013-225 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Cryptogramma crispa 14221 Zion 2012-023 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains:

13 Cystopteris fragilis 14260 Marx 2013-166 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Cystopteris fragilis 14588 Marx 2013-287 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Cystopteris fragilis 14220 Zion 2012-022 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountians: Dasiphora fruticosa 14415 Marx 2013-209 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Deschampsia cespitosa subsp cespitosa 14407 Marx 2013-201 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Deschampsia cespitosa subsp cespitosa 14585 Marx 2013-284 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Deschampsia cespitosa subsp cespitosa 14662 Marx 2013-363 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Draba cf crassifolia 13569 Marx 2012-036 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Draba cf crassifolia 14218 Zion 2012-020 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Draba cf densifolia 14241 Marx 2013-146 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Draba cf densifolia 14605 Marx 2013-304 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Draba cf lonchocarpa 14268 Marx 2013-174 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Draba cf oreibata 14404 Marx 2013-136 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: White Cloud Mountains: Draba cf stenoloba NA Marx 2013-197 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Draba lonchocarpa 14455 Marx 2013-253 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Draba lonchocarpa 14610 Marx 2013-309 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Draba lonchocarpa 14629 Marx 2013-328 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit 14 Idaho: Sawtooth National Forest: White Cloud Mountains: Draba lonchocarpa 14690 Marx 2013-393 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Draba lonchocarpa 14694 Marx 2013-397 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Draba oligosperma NA Marx 2012-014 (a-d) July 31, 2012 Thompson Peak: South facing gravel slope just above camp 44.14639 -115.00528 at Profile Lake Idaho: Sawtooth National Forest: Pioneer Mountains: Draba oligosperma 14604 Marx 2013-303 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Draba oligosperma 14688 Marx 2013-391 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Draba oligosperma 14703 Marx 2013-406 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Draba oreibata 14335 Marx 2013-104 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Sawtooth Mountains: Draba oreibata 14217 Zion 2012-019 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Elymus elymoides 14356 Marx 2013-127 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: White Cloud Mountains: Elymus elymoides 14251 Marx 2013-157 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Elymus elymoides 14439 Marx 2013-236 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Elymus elymoides 14636 Marx 2013-336 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Elymus elymoides 14225 Zion 2012-027 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Elymus scribneri 14673 Marx 2013-375 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Elymus scribneri NA Marx 2013-443 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Epilobium alpinum 14301 Marx 2013-070 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline 15 Idaho: Sawtooth National Forest: Sawtooth Mountians: Epilobium alpinum 14339 Marx 2013-108 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Epilobium alpinum 14252 Marx 2013-158 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Epilobium alpinum 14409 Marx 2013-203 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Epilobium alpinum 14441 Marx 2013-239 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Epilobium alpinum 14675 Marx 2013-377 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Epilobium alpinum var clavatum 14123 Zion 2012-001 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Epilobium obcordatum 14130 Zion 2012-008 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountians: Eremogone aculeata 14438 Marx 2013-235 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Eremogone aculeata 14663 Marx 2013-364 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Pioneer Mountians: Eremogone congesta NA Marx 2013-221 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Eremogone congesta 14589 Marx 2013-288 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eremogone congesta 14670 Marx 2013-372 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Ericameria discoidea 14315 Marx 2013-084 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Erigeron asperugineus 14247 Marx 2013-152 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron compositus 13537 Marx 2012-001 July 31, 2012 44.14369 -115.00683 Thompson Peak: Just below summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron compositus 14009 Marx 2012-056 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline 16 Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron compositus 14307 Marx 2013-076 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron compositus 14355 Marx 2013-126 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: White Cloud Mountains: Erigeron compositus 14248 Marx 2013-153 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Erigeron compositus 14452 Marx 2013-250 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Erigeron compositus 14623 Marx 2013-322 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Erigeron compositus 14700 Marx 2013-403 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron evermannii 13578 Marx 2012-045 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Erigeron evermannii 14340 Marx 2013-109 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Sawtooth Mountians: Erigeron evermannii 14363 Marx 2013-135 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron evermannii 14224 Zion 2012-026 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron grandiflorus 14012 Clevenger 2012-003 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Erigeron grandiflorus 14310 Marx 2013-079 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

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Idaho: Sawtooth National Forest: Pioneer Mountains: Erigeron grandiflorus 14600 Marx 2013-299 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Erigeron lonchophyllus 14612 Marx 2013-311 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Erigeron sp 14667 Marx 2013-369 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum crosbyae 13541 Marx 2012-006 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit 17 Idaho: Sawtooth National Forest: Sawtooth Mountians: Eriogonum crosbyae 14350 Marx 2013-119 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum crosbyae 14242 Marx 2013-147 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Eriogonum crosbyae 14607 Marx 2013-306 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum crosbyae 14681 Marx 2013-384 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum flavum var piperi 14650 Marx 2013-351 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum flavum var piperi 14654 Marx 2013-355 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium 14327 Marx 2013-096 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Eriogonum ovalifolium 14344 Marx 2013-113 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Sawtooth Mountians: Eriogonum ovalifolium 14349 Marx 2013-118 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Sawtooth Mountians: Eriogonum ovalifolium 14366 Marx 2013-140 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum ovalifolium 14244 Marx 2013-149 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum ovalifolium 14280 Marx 2013-186 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium var depressum 13542 Marx 2012-007 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium var depressum 13543 Marx 2012-008 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium var depressum 13564 Marx 2012-031 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Eriogonum ovalifolium var depressum 14450 Marx 2013-248 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit 18 Idaho: Sawtooth National Forest: Pioneer Mountains: Eriogonum ovalifolium var depressum 14602 Marx 2013-301 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum ovalifolium var depressum 14649 Marx 2013-350 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Eriogonum ovalifolium var depressum 14702 Marx 2013-405 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium var purpureum 14131 Zion 2012-009 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum ovalifolium var purpureum 14133 Zion 2012-011 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum pyrolaefolium var coryphaeum 13567 Marx 2012-034 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Eriogonum pyrolaefolium var coryphaeum 14126 Zion 2012-004 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Eritrichium nanum 13538 Marx 2012-002 July 31, 2012 44.14369 -115.00683 Thompson Peak: Just below summit Idaho: Sawtooth National Forest: White Cloud Mountains: Eritrichium nanum 14710 Marx 2013-413 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eritrichium nanum 14711 Marx 2013-414 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eritrichium nanum 14712 Marx 2013-415 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Eritrichium nanum 14713 Marx 2013-416 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Erysimum asperum 14302 Marx 2013-071 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Erysimum asperum 14235 Zion 2012-037 August 7, 2012 44.11012 -115.00285 Horstmann Peak: treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Erythranthe tilingii 14413 Marx 2013-207 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Erythranthe tilingii 14574 Marx 2013-273 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Festuca brachyphylla subsp brachyphylla 13566 Marx 2012-033 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline 19 Idaho: Sawtooth National Forest: Sawtooth Mountains: Festuca brachyphylla subsp brachyphylla 14318 Marx 2013-087 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Festuca brachyphylla subsp brachyphylla NA Marx 2013-156 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Festuca brachyphylla subsp brachyphylla 14262 Marx 2013-168 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Festuca brachyphylla subsp brachyphylla 14640 Marx 2013-340 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Gentiana calycosa NA Marx 2012-025 July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Pioneer Mountians: Gentiana calycosa 14414 Marx 2013-208 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Gentiana calycosa 14577 Marx 2013-276 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Gentiana calycosa 14645 Marx 2013-346 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Gentiana calycosa var obtusiloba 14032 Clevenger 2012-025 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountians: Haplopappus macronema 14342 Marx 2013-111 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Haplopappus macronema 14274 Marx 2013-180 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Haplopappus macronema 15004 Marx 2013-180 REXTRACTION 44.04019 -114.58878

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountains: Haplopappus macronema 14626 Marx 2013-325 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Haplopappus suffruticosus 14657 Marx 2013-358 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Haplopappus suffruticosus 15008 Marx 2013-419 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Heuchera parvifolia var dissecta 14320 Marx 2013-089 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline 20 Idaho: Sawtooth National Forest: Sawtooth Mountians: Heuchera parvifolia var dissecta 14346 Marx 2013-115 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Heuchera parvifolia var dissecta 14250 Marx 2013-155 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Heuchera parvifolia var dissecta 14449 Marx 2013-247 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Heuchera parvifolia var dissecta 14625 Marx 2013-324 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Heuchera parvifolia var dissecta 14216 Zion 2012-018 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Hieracium triste 14017 Clevenger 2012-008 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Hieracium triste 14299 Marx 2013-068 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Hulsea algida 13545 Marx 2012-010 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Hulsea algida 13575 Marx 2012-042 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Hulsea algida 14321 Marx 2013-090 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Hulsea algida 14365 Marx 2013-139 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Hulsea algida 14276 Marx 2013-182 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Hulsea algida 14459 Marx 2013-257 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Hulsea algida 14627 Marx 2013-326 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Hulsea algida 14222 Zion 2012-024 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Ivesia gordonii 14024 Clevenger 2012-017 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 21 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Ivesia gordonii 13559 Marx 2012-026 July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountians: Ivesia gordonii 14347 Marx 2013-116 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: Pioneer Mountains: Ivesia gordonii 14613 Marx 2013-312 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Ivesia gordonii 14664 Marx 2013-365 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Juncus drummondii 14025 Clevenger 2012-018 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Juncus drummondii 14324 Marx 2013-093 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Juncus drummondii 14590 Marx 2013-289 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Juncus drummondii 14129 Zion 2012-007 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Juncus paryii 14325 Marx 2013-094 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Juncus paryii NA Marx 2013-133 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Juncus paryii 14263 Marx 2013-169 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Juncus paryii 14440 Marx 2013-238 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Juncus paryii 14658 Marx 2013-359 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Juncus paryii 14228 Zion 2012-030 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Juniperus communis 14031 Clevenger 2012-024 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 22 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Juniperus communis 14330 Marx 2013-099 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Juniperus communis 14285 Marx 2013-191 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Juniperus communis 14233 Zion 2012-035 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Lewisia pygmaea var pygmara 14010 Clevenger 2012-001 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Pioneer Mountains: Lewisia pygmaea var pygmara 14592 Marx 2013-291 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Lomatium idahoense 14134 Zion 2012-012 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Lomatium idahoense 14246 Marx 2013-151 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Lupinus argenteus var depressus 14286 Marx 2013-192 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Lupinus argenteus var depressus 14408 Marx 2013-202 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Lupinus argenteus var depressus 14647 Marx 2013-348 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Luzula piperi 13572 Marx 2012-039 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Luzula piperi 14295 Marx 2013-063 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Luzula spicata 13547 Marx 2012-012 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Luzula spicata 14003 Marx 2012-050 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Luzula spicata 14269 Marx 2013-175 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Luzula spicata 14637 Marx 2013-337 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit 23 Idaho: Sawtooth National Forest: Pioneer Mountains: Minuartia cf obtusiloba 14606 Marx 2013-305 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Minuartia cf obtusiloba 14686 Marx 2013-389 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Minuartia nuttallii 13544 Marx 2012-009 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Minuartia nuttallii 14305 Marx 2013-074 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Minuartia nuttallii 14697 Marx 2013-400 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Minuartia obtusiloba NA Marx 2012-021a July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Minuartia obtusiloba 13565 Marx 2012-032 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Minuartia obtusiloba 14304 Marx 2013-073 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Minuartia obtusiloba 14249 Marx 2013-154 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Minuartia obtusiloba 14608 Marx 2013-307 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Minuartia obtusiloba 14621 Marx 2013-320 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Minuartia obtusiloba 14679 Marx 2013-381 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: White Cloud Mountains: Minuartia obtusiloba 14705 Marx 2013-408 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Musineon divaricatum 14236 Zion 2012-038 August 7, 2012 44.11012 -115.00285 Horstmann Peak: treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Oxyria digyna 13580 Marx 2012-047 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Oxyria digyna 14329 Marx 2013-098 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline 24 Idaho: Sawtooth National Forest: Sawtooth Mountians: Oxyria digyna 14343 Marx 2013-112 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Oxyria digyna 14257 Marx 2013-163 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Oxyria digyna 14445 Marx 2013-243 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Oxyria digyna 14631 Marx 2013-330 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Oxyria digyna 14696 Marx 2013-399 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Oxyria digyna 14125 Zion 2012-003 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Oxytropis cf deflexa 14678 Marx 2013-380 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Oxytropis cf deflexa 14691 Marx 2013-394 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Oxytropis parryi 14695 Marx 2013-398 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Packera subnuda 14580 Marx 2013-279 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Parnassia fimbriata 14370 Marx 2013-200 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Pedicularis groenlandica 14410 Marx 2013-204 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountains: Pedicularis groenlandica 14596 Marx 2013-295 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Penstemon montanus var idahoensis 13570 Marx 2012-037 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Penstemon montanus var idahoensis 14279 Marx 2013-185 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Penstemon montanus var idahoensis 14234 Zion 2012-036 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW 25 Idaho: Sawtooth National Forest: Pioneer Mountians: Penstemon procerus var formosus 14447 Marx 2013-245 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Penstemon sp 14676 Marx 2013-378 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Phacelia hastata 14245 Marx 2013-150 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Phacelia hastata 14457 Marx 2013-255 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Phleum alpinum 14423 Marx 2013-217 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Phleum alpinum 14594 Marx 2013-293 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Phlox cf diffusa 14624 Marx 2013-323 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Phlox cf pulvinata 14254 Marx 2013-160 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Phlox cf pulvinata 14461 Marx 2013-260 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Phlox cf pulvinata 15007 Marx 2013-418 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Phlox pulvinata 14020 Clevenger 2012-013 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: White Cloud Mountains: Phlox pulvinata 14669 Marx 2013-371 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Phlox pulvinata 14215 Zion 2012-017 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Phyllodoce empetriformis 14021 Clevenger 2012-014 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Phyllodoce empetriformis 14291 Marx 2013-059 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians:

26 Phyllodoce empetriformis 14359 Marx 2013-130 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: Pioneer Mountians: Phyllodoce empetriformis 14412 Marx 2013-206 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Phyllodoce empetriformis 14422 Marx 2013-216 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Phyllodoce empetriformis 14597 Marx 2013-296 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Phyllodoce empetriformis 14219 Zion 2012-021 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Phyllodoce glanduliflora 14023 Clevenger 2012-016 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Phyllodoce glanduliflora 14290 Marx 2013-058 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Phyllodoce glanduliflora 14581 Marx 2013-280 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Picea engelmannii 13571 Marx 2012-038 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Pinus albicaulis 14030 Clevenger 2012-023 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Pinus albicaulis 13573 Marx 2012-040 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Pinus albicaulis NA Marx 2013-121 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit)

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Pinus albicaulis 14231 Zion 2012-033 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountians: Poa fendleriana subsp fendleriana 14367 Marx 2013-141 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Poa fendleriana subsp fendleriana 14282 Marx 2013-188 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Poa fendleriana subsp fendleriana 14132 Zion 2012-010 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW 27 Idaho: Sawtooth National Forest: Sawtooth Mountains: Poa secunda NA Clevenger 2012-011 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Poa secunda 13568 Marx 2012-035 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Poa secunda 14007 Marx 2012-054 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Poa secunda 14357 Marx 2013-128 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: White Cloud Mountains: Poa secunda 14281 Marx 2013-187 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Poa secunda NA Marx 2013-237 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Poa secunda 14462 Marx 2013-261 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Poa secunda 14642 Marx 2013-342 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Poa secunda 14643 Marx 2013-343 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Poa secunda 14674 Marx 2013-376 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Polemonium viscosum 13554 Marx 2012-019 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Polemonium viscosum 14353 Marx 2013-123 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: White Cloud Mountains: Polemonium viscosum 14271 Marx 2013-177 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Polemonium viscosum 14467 Marx 2013-266 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Polemonium viscosum 14633 Marx 2013-332 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Polemonium viscosum 14698 Marx 2013-401 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline 28 Idaho: Sawtooth National Forest: Pioneer Mountains: Polygonum viviparum 14573 Marx 2013-272 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Potentilla brevifolia 14015 Clevenger 2012-006 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Potentilla brevifolia 14328 Marx 2013-097 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Potentilla glandulosa 14314 Marx 2013-083 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Potentilla glandulosa 14405 Marx 2013-138 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Potentilla glandulosa 14255 Marx 2013-161 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Potentilla glandulosa 14442 Marx 2013-240 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Potentilla glandulosa 14634 Marx 2013-333 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Potentilla glandulosa var glandulosa 14230 Zion 2012-032 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Potentilla glaucophylla var glaucophylla 15002 Marx 2012-028 REXTRACTION 44.14139 -115.01358 Idaho: Sawtooth National Forest: Sawtooth Mountains: Potentilla glaucophylla var glaucophylla 13561 Marx 2012-028 July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Pioneer Mountians: Potentilla glaucophylla var glaucophylla 14426 Marx 2013-222 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit

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MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountains: Potentilla glaucophylla var glaucophylla 14572 Marx 2013-271 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Potentilla glaucophylla var glaucophylla 14593 Marx 2013-292 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Potentilla glaucophylla var glaucophylla 15005 Marx 2013-292 REXTRACTION 43.74357 -114.12403 Potentilla glaucophylla var glaucophylla 15006 Marx 2013-352 REXTRACTION 44.10969 -114.63886 Idaho: Sawtooth National Forest: White Cloud Mountains:

29 Potentilla glaucophylla var glaucophylla 14651 Marx 2013-352 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Ranunculus eschscholtzii 14239 Marx 2013-144 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Ranunculus eschscholtzii var eschscholtzii 14027 Clevenger 2012-020 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Ribes lacustre 14002 Marx 2012-049 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Ribes lacustre 14259 Marx 2013-165 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Ribes lacustre 14436 Marx 2013-233 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Ribes lacustre 14618 Marx 2013-317 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Salix arctica 14421 Marx 2013-215 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Salix arctica 14578 Marx 2013-277 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Salix nivalis 14660 Marx 2013-361 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Saxifraga bronchialis 14006 Marx 2012-053 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Saxifraga ferruginea 14270 Marx 2013-176 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Saxifraga mertensiana 14424 Marx 2013-218 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountains: Saxifraga mertensiana 14570 Marx 2013-269 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Saxifraga oppositifolia 14708 Marx 2013-411 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Sedum debile 14272 Marx 2013-178 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Sedum debile 14656 Marx 2013-357 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow 30 Idaho: Sawtooth National Forest: Sawtooth Mountains: Sedum lanceolatum var lanceolatum 13539 Marx 2012-003 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Sedum lanceolatum var lanceolatum 15001 Marx 2012-003 REXTRACTION 44.14139 -115.01 Idaho: Sawtooth National Forest: Sawtooth Mountains: Sedum lanceolatum var lanceolatum 13576 Marx 2012-043 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Sedum lanceolatum var lanceolatum 14300 Marx 2013-069 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Sedum lanceolatum var lanceolatum 14444 Marx 2013-242 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Sedum lanceolatum var lanceolatum 14611 Marx 2013-310 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Sedum lanceolatum var lanceolatum 14655 Marx 2013-356 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Sedum lanceolatum var lanceolatum 14137 Zion 2012-015 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Selaginella densa 14354 Marx 2013-125 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: White Cloud Mountains: Selaginella densa 14265 Marx 2013-171 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Selaginella watsonii NA Marx 2013-259 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Senecio fremontii 14033 Clevenger 2012-026 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Senecio fremontii 14005 Marx 2012-052 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountians: Senecio fremontii 14334 Marx 2013-103 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio fremontii 14253 Marx 2013-159 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio fremontii 14275 Marx 2013-181 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians:

31 Senecio fremontii 14466 Marx 2013-265 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Senecio fremontii 14616 Marx 2013-315 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio fremontii 14707 Marx 2013-410 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Senecio fremontii 14127 Zion 2012-005 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountains: Senecio residifolius 14579 Marx 2013-278 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Senecio werneriifolius 14338 Marx 2013-107 July 22, 2013 Snowyside Peak: along west face and south ridge line (trail to 43.93825 -114.97142 summit) Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio werneriifolius 14243 Marx 2013-148 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio werneriifolius 14277 Marx 2013-183 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio werneriifolius 14671 Marx 2013-373 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio werneriifolius 14672 Marx 2013-374 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Senecio werneriifolius 14685 Marx 2013-388 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Sibbaldia procumbens 14014 Clevenger 2012-005 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Sibbaldia procumbens 13574 Marx 2012-041 August 10, 2012 44.09333 -115.99681 Braxon Peak: Summit down south face to treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Sibbaldia procumbens 14323 Marx 2013-092 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Sibbaldia procumbens 14256 Marx 2013-162 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Sibbaldia procumbens 14425 Marx 2013-219 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Sibbaldia procumbens 15009 Marx 2013-420 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline 32 Idaho: Sawtooth National Forest: White Cloud Mountains: Sibbaldia procumbens 15010 Marx 2013-421 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: Sawtooth Mountains: Sibbaldia procumbens 14227 Zion 2012-029 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Sawtooth Mountains: Silene acaulis 13555 Marx 2012-020 July 31, 2012 Thompson Peak: Meadow just at base of west side of 44.14139 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Sawtooth Mountains: Smelowskia calycina 13550 Marx 2012-004 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Smelowskia calycina 14352 Marx 2013-122 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: Pioneer Mountians: Smelowskia calycina 14465 Marx 2013-264 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountians: Smelowskia calycina 14469 Marx 2013-268 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Smelowskia calycina 14617 Marx 2013-316 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Smelowskia calycina 14693 Marx 2013-396 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Solidago multiradiata 14419 Marx 2013-213 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Solidago multiradiata 14595 Marx 2013-294 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Solidago multiradiata 14653 Marx 2013-354 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Sawtooth Mountains: Solidago multiradiata 14124 Zion 2012-002 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: White Cloud Mountains: Stenotus acaulis 14278 Marx 2013-184 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Stenotus acaulis 14666 Marx 2013-368 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Stenotus acaulis 14704 Marx 2013-407 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline 33 Idaho: Sawtooth National Forest: Sawtooth Mountains: Synthyris pinnatifida var cenescens 14028 Clevenger 2012-021 August 1, 2012 Thompson Peak: Meadow just at base of west side of 44.14219 -115.01358 mountain, just south of a snowpack Idaho: Sawtooth National Forest: Pioneer Mountians: Taraxacum lyratum 14443 Marx 2013-241 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Taraxacum lyratum 14615 Marx 2013-314 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Tonestus lyallii 14308 Marx 2013-077 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountians: Tonestus lyallii 14362 Marx 2013-134 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected at second treeline only Idaho: Sawtooth National Forest: White Cloud Mountains: Tonestus lyallii 14264 Marx 2013-170 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Tonestus lyallii 14622 Marx 2013-321 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Trisetum spicatum 13552 Marx 2012-017 July 31, 2012 44.14139 -115.01 Thompson Peak: SW slope heading down from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Trisetum spicatum 14313 Marx 2013-082 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Trisetum spicatum 14317 Marx 2013-086 July 19, 2013 44.01108 -114.98117 Mount Cramer: east ridge from summit to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Trisetum spicatum 14358 Marx 2013-129 July 22, 2013 43.93825 -114.97142 Snowyside Peak: collected on summit Idaho: Sawtooth National Forest: White Cloud Mountains: Trisetum spicatum 14261 Marx 2013-167 July 25, 2013 44.04019 -114.58878 Castle Peak: summit down southwest slope to treeline

Continued on next page Table Appendix S2 – continued from previous page

MiSeq Species Accession Voucher Number Date Collected Locality WGS N WGS W Number

Idaho: Sawtooth National Forest: Pioneer Mountians: Trisetum spicatum 14411 Marx 2013-205 August 7, 2013 43.76619 -114.15839 Salzburger Spitzl: Meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountians: Trisetum spicatum 14463 Marx 2013-262 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: Pioneer Mountains: Trisetum spicatum 14639 Marx 2013-339 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Trisetum spicatum 14652 Marx 2013-353 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow 34 Idaho: Sawtooth National Forest: White Cloud Mountains: Trisetum spicatum 14677 Marx 2013-379 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Trisetum spicatum 14692 Marx 2013-395 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Trisetum spicatum NA Marx 2013-444 August 9, 2013 43.74938 -114.1312 Hyndman Peak: south facing talus/boulder field from summit Idaho: Sawtooth National Forest: Sawtooth Mountains: Trisetum spicatum 14138 Zion 2012-016 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountians: Unknown 14437 Marx 2013-234 August 7, 2013 43.77446 -114.15942 Salzburger Spitzl: talus down from summit Idaho: Sawtooth National Forest: White Cloud Mountains: Unknown NA Marx 2013-367 August 14, 2013 44.10969 -114.63886 D.O. Lee Peak: collected in alpine meadow Idaho: Sawtooth National Forest: White Cloud Mountains: Unknown NA Marx 2013-383 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: White Cloud Mountains: Unknown 14706 Marx 2013-409 August 14, 2013 44.10277 -114.62859 D.O. Lee Peak: in talus, north face then west to treeline Idaho: Sawtooth National Forest: Sawtooth Mountains: Unknown 14135 Zion 2012-013 August 7, 2012 44.1117 -115.00318 Horstmann Peak: Just below summit to the SW Idaho: Sawtooth National Forest: Pioneer Mountains: Veronica wormskjoldii 14587 Marx 2013-286 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Idaho: Sawtooth National Forest: Pioneer Mountains: Viola cf adunca 14576 Marx 2013-275 August 9, 2013 43.74357 -114.12403 Hyndman Peak: in meadow above treeline Table Appendix S3: Accession numbers for the high-throughput (miseq) or GenBank (ncbi) sequences used for each gene region. Table indicates the presence (X) or absence (-) of each species on each of the nine alpine summits sampled across the the Sawtooth National Forest, and if the species was collected from a high-alpine meadow on that summit (“Meadow”).

Species ITS trnTLF rbcL matK atpB ndhF MeadowBraxonCastleD.O. PeakHorstmann Peak LeeHyndmanMount PeakSalzburgerSnowyside Peak CramerPeakThompson Spitzl Peak Peak

Abies lasiocarpa NA miseq|14029 ncbi|56236673 ncbi|6520431 miseq|14232 NA X --- X --- XX Achillea millefolium var alpicola miseq|14619 miseq|14136 NA miseq|14136 NA miseq|14136 X - XXXX - X -- Aconogonon phytolaccaefolium NA NA NA miseq|14229 miseq|14229 NA - - - - X ----- Agoseris aurantiaca ncbi|33669310 NA NA NA NA NA X ---- X ---- Agrostis variabilis NA NA NA NA NA NA X ------X -- Androsace septentrionalis ncbi|30526256 ncbi|30526129 miseq|14464 miseq|14464 NA miseq|14464 - - - X - X - X -- Anemone drummondii miseq|14309 NA ncbi|459991296 ncbi|459927014 miseq|14309 NA - - - X -- X --- Anemone drummondii var drummondii miseq|13562 miseq|13562 NA NA miseq|13562 NA - X ------Antennaria alpina miseq|14019 miseq|14019 miseq|14360 miseq|14019 miseq|14322 miseq|14019 X - XX - XX - XX Antennaria corymbosa miseq|14418 miseq|14418 miseq|14418 miseq|14418 miseq|14418 miseq|14418 X ------X -- 35 Antennaria lanata miseq|14016 miseq|14016 NA miseq|14016 NA miseq|14016 X ----- X -- X Antennaria microphylla miseq|14004 miseq|14004 miseq|14348 miseq|14004 miseq|14004 miseq|14004 - X ------X - Antennaria rosea NA miseq|14273 NA miseq|14273 NA miseq|14273 - - X ------Antennaria umbrinella miseq|13540 miseq|13540 miseq|14446 miseq|13540 NA miseq|13540 - - - - XX - X - X Aquilegia flavescens NA NA NA miseq|14428 miseq|14428 NA ------X -- Arabis lemmonii miseq|14266 NA miseq|14683 miseq|14266 miseq|14266 miseq|14266 X - XX - X - XXX Arabis microphylla miseq|14011 miseq|14011 miseq|14011 miseq|14011 miseq|14011 miseq|14011 X - X --- X -- X Arnica chamissonis miseq|14416 NA NA miseq|14416 NA miseq|14416 X ---- X - X -- Arnica latifolia miseq|14361 NA miseq|14361 miseq|14303 miseq|14316 miseq|14303 - - X -- XX - X - Arnica latifolia var gracilis miseq|13577 miseq|13577 NA miseq|13577 NA miseq|13577 - X -- X ----- Arnica mollis NA ncbi|34765606 ncbi|34765359 miseq|14453 NA miseq|14453 ------X -- Arnica parryi miseq|14240 miseq|14240 NA miseq|14240 NA miseq|14240 - - X ------Artemisia dracunculus NA miseq|14284 NA miseq|14284 NA miseq|14284 - - X ------Aster alpinus miseq|14609 miseq|14609 NA miseq|14609 miseq|14609 miseq|14609 - - - - - X ---- Aster foliaceus var apricus miseq|14583 NA NA miseq|14430 miseq|14430 miseq|14430 X -- X - X - X -- Aster occidentalis var occidentalis miseq|14420 NA NA miseq|14420 NA miseq|14420 X ------X -- Astragalus alpinus miseq|14427 ncbi|260079530 ncbi|817991696 miseq|14427 miseq|14427 miseq|14427 ------X -- Astragalus eucosmus miseq|14598 miseq|14598 miseq|14598 miseq|14598 miseq|14598 miseq|14598 - - - - - X ---- Astragalus kentrophyta miseq|14456 miseq|14456 miseq|14620 miseq|14456 miseq|14701 miseq|14456 - - - X - X - X -- Bistorta bistortoides NA NA miseq|14432 miseq|14417 miseq|14417 miseq|14417 X ---- X - X -- Bupleurum americanum miseq|13560 miseq|13560 miseq|14364 miseq|14364 miseq|14364 miseq|13560 X ------XX Calamagrostis purpurescens miseq|13551 miseq|13551 NA NA miseq|14319 miseq|13551 - - - - - XX -- X Carex luzulina ncbi|132694270 ncbi|121645904 NA NA miseq|14458 NA ------X -- Carex microptera ncbi|60328071 NA NA NA miseq|14638 NA - - - - - X ---- Carex nardina ncbi|300411420 miseq|13549 miseq|14026 NA miseq|13549 NA X -- X - X --- X Carex pachycarpa NA NA NA NA NA NA ------X --- Carex proposita NA NA NA NA NA NA ------X - Carex scopulorum miseq|14018 miseq|14018 NA NA miseq|14018 NA X ------X

Continued on next page Table Appendix S3 – continued from previous page

Species ITS trnTLF rbcL matK atpB ndhF MeadowBraxonCastleD.O. PeakHorstmann Peak LeeHyndmanMount PeakSalzburgerSnowyside Peak CramerPeakThompson Spitzl Peak Peak

Carex sp NA NA NA NA NA NA ------X Carex straminiformis miseq|14468 miseq|13548 miseq|14635 NA miseq|13548 miseq|14635 XX - XXX - X - X Cassiope mertensiana var gracilis miseq|14022 miseq|14022 NA miseq|14022 miseq|14022 miseq|14022 X -- X -- X -- X Castilleja applegatei NA NA NA miseq|14644 NA miseq|14644 - - X ------Castilleja cusickii ncbi|129926134 miseq|14238 NA miseq|14238 miseq|14238 miseq|14238 X - X --- XX -- Castilleja miniata ncbi|129926152 miseq|14289 NA miseq|14289 miseq|14289 miseq|14289 X ---- XXX -- Castilleja rhexiifolia NA ncbi|225134744 NA miseq|14406 miseq|14406 miseq|14406 X ------X -- Cerastium arvense ncbi|598699206 miseq|14575 ncbi|667668196 ncbi|62870790 NA miseq|14575 X ---- X ---- Cerastium beeringianum miseq|14628 ncbi|111380067 miseq|14628 miseq|14687 miseq|14628 miseq|14451 - - - X - X - X -- Chaenactis alpina miseq|13553 miseq|13553 NA miseq|13553 miseq|14297 miseq|13553 - XX --- XXXX Chionophila tweedyi miseq|14008 miseq|14008 miseq|14008 miseq|14008 miseq|14008 miseq|14008 XX ---- X -- X Cirsium scariosum NA ncbi|559146864 ncbi|500049589 miseq|14435 NA miseq|14435 ------X -- Cirsium subniveum ncbi|21636093 NA NA miseq|14288 miseq|14288 miseq|14288 - - X ------Cistanthe umbellata NA NA NA miseq|14434 miseq|14434 miseq|14434 ------X -- Claytonia megarhiza NA miseq|14267 miseq|14614 miseq|14267 miseq|14267 miseq|14267 - - X -- XXXX - 36 Claytonia megarhiza var bellidifolia NA miseq|14034 NA miseq|14034 miseq|14001 miseq|14001 XX ------X Collomia debilis miseq|14336 NA miseq|14336 miseq|14336 NA miseq|14336 - - - X ---- X - Crepis nana ncbi|170284000 ncbi|260079528 ncbi|459991575 miseq|14699 NA miseq|14699 - - - X ------Cryptogramma crispa NA NA ncbi|459653136 NA ncbi|149798674 NA - - - - X - XXX - Cystopteris fragilis NA ncbi|17063804 ncbi|373222139 NA ncbi|373222274 NA X - X - XX ---- Dasiphora fruticosa ncbi|9392379 ncbi|19032449 ncbi|290586169 miseq|14415 miseq|14415 miseq|14415 X ------X -- Deschampsia cespitosa subsp cespitosa miseq|14662 miseq|14585 NA NA miseq|14407 miseq|14407 X -- X - X - X -- Draba crassifolia miseq|13569 miseq|13569 miseq|13569 miseq|13569 miseq|13569 miseq|13569 - X -- X ----- Draba densifolia miseq|14241 ncbi|94537846 miseq|14605 NA miseq|14241 miseq|14241 - - X -- X ---- Draba lonchocarpa miseq|14610 miseq|14610 miseq|14610 NA miseq|14268 miseq|14268 - - XX - X - X -- Draba oligosperma miseq|14604 ncbi|94537769 miseq|14604 ncbi|459928282 NA miseq|14604 - - - X - X ---- Draba oreibata NA miseq|14404 miseq|14335 miseq|14217 miseq|14217 miseq|14217 - - - - X --- X - Draba stenoloba ncbi|94538264 ncbi|58978832 NA NA NA NA - - X ------Elymus elymoides miseq|14636 miseq|14251 miseq|14251 NA miseq|14225 miseq|14225 - - X - XX - XX - Elymus scribneri miseq|14673 miseq|14673 miseq|14673 NA NA miseq|14673 - - - X --- X -- Epilobium alpinum miseq|14339 NA miseq|14252 miseq|14252 miseq|14252 miseq|14252 X - XX -- XXX - Epilobium alpinum var clavatum miseq|14123 miseq|14123 miseq|14123 miseq|14123 miseq|14123 miseq|14123 - - - - X ----- Epilobium obcordatum miseq|14130 miseq|14130 NA miseq|14130 miseq|14130 miseq|14130 - - - - X ----- Eremogone aculeata miseq|14438 miseq|14438 NA miseq|14438 miseq|14438 miseq|14438 X -- X --- X -- Eremogone congesta miseq|14670 miseq|14589 miseq|14670 miseq|14589 miseq|14589 miseq|14589 X -- X - X - X -- Ericameria discoidea NA NA NA miseq|14315 NA miseq|14315 ------X --- Erigeron asperugineus NA NA NA miseq|14247 NA miseq|14247 - - X ------Erigeron compositus miseq|14009 miseq|13537 miseq|14009 miseq|13537 miseq|14009 miseq|13537 - XXX - XXXXX Erigeron evermannii miseq|13578 miseq|13578 NA miseq|13578 miseq|14224 miseq|13578 - X -- X --- X - Erigeron grandiflorus miseq|14012 miseq|14012 NA miseq|14012 miseq|14012 miseq|14012 X ---- XX -- X Erigeron lonchophyllus miseq|14612 miseq|14612 miseq|14612 miseq|14612 miseq|14612 miseq|14612 - - - - - X ---- Erigeron sp miseq|14667 NA NA miseq|14667 NA miseq|14667 X -- X ------Eriogonum crosbyae NA miseq|13541 miseq|14350 miseq|14242 miseq|14242 miseq|13541 - - XX - X -- XX

Continued on next page Table Appendix S3 – continued from previous page

Species ITS trnTLF rbcL matK atpB ndhF MeadowBraxonCastleD.O. PeakHorstmann Peak LeeHyndmanMount PeakSalzburgerSnowyside Peak CramerPeakThompson Spitzl Peak Peak

Eriogonum flavum var piperi NA NA NA miseq|14650 miseq|14650 miseq|14650 X -- X ------Eriogonum ovalifolium miseq|14327 NA miseq|14349 miseq|14244 miseq|14244 miseq|14244 - - X --- X - X - Eriogonum ovalifolium var depressum NA miseq|13542 miseq|14702 miseq|13564 miseq|13542 miseq|13542 XX - X - X - X - X Eriogonum ovalifolium var purpureum ncbi|397913667 miseq|14133 NA miseq|14133 miseq|14133 miseq|14133 - - - - X ----- Eriogonum pyrolaefolium var coryphaeum miseq|14126 miseq|13567 miseq|14126 miseq|13567 miseq|13567 miseq|13567 - X -- X ----- Eritrichium nanum miseq|14711 ncbi|399606334 NA miseq|14711 miseq|14712 miseq|14711 - - - X ----- X Erysimum asperum ncbi|9957688 ncbi|312182215 miseq|14302 miseq|14235 miseq|14235 miseq|14235 - - - - X - X --- Erythranthe tilingii ncbi|46392999 miseq|14413 NA miseq|14413 miseq|14413 miseq|14413 X ---- X - X -- Festuca brachyphylla subsp brachyphylla miseq|14318 miseq|13566 NA NA miseq|13566 miseq|13566 - XX -- XX --- Gentiana calycosa miseq|14414 miseq|14577 miseq|14577 miseq|14414 miseq|14414 miseq|14414 X -- X - X - X - X Gentiana calycosa var obtusiloba miseq|14032 miseq|14032 NA miseq|14032 NA miseq|14032 X ------X Haplopappus macronema miseq|14626 NA miseq|14626 miseq|14274 miseq|14274 miseq|14274 - - X -- X -- X - Haplopappus suffruticosus miseq|14657 NA NA miseq|14657 NA miseq|14657 X - XX ------Heuchera parvifolia var dissecta miseq|14625 NA miseq|14216 miseq|14216 miseq|14216 miseq|14216 - - X - XXXXX - Hieracium triste miseq|14017 miseq|14017 NA miseq|14017 miseq|14017 miseq|14017 X ----- X -- X 37 Hulsea algida miseq|13545 miseq|13545 miseq|14365 miseq|13545 miseq|13575 miseq|13545 - XX - XXXXXX Ivesia gordonii miseq|13559 miseq|13559 miseq|14613 miseq|13559 miseq|14024 miseq|13559 X -- X - X -- XX Juncus drummondii NA miseq|14129 miseq|14025 NA miseq|14129 miseq|14025 X --- XXX -- X Juncus paryii miseq|14440 miseq|14263 miseq|14658 NA miseq|14228 NA X - XXX - XXX - Juniperus communis NA miseq|14031 NA NA ncbi|56236589 ncbi|62467606 X - X - X - X -- X Lewisia pygmaea var pygmara NA miseq|14010 NA miseq|14010 miseq|14010 miseq|14010 X ---- X --- X Lomatium idahoense miseq|14134 miseq|14134 NA miseq|14134 NA miseq|14134 - - X - X ----- Lupinus argenteus var depressus miseq|14647 NA NA miseq|14408 miseq|14647 miseq|14408 X - XX --- X -- Luzula piperi miseq|13572 miseq|13572 miseq|13572 NA miseq|13572 NA - X ---- X --- Luzula spicata miseq|13547 miseq|13547 miseq|14637 NA miseq|14003 NA - XX -- X --- X Minuartia nuttallii miseq|13544 NA NA miseq|13544 miseq|13544 miseq|13544 - - - X -- X -- X Minuartia obtusiloba miseq|13565 ncbi|374351334 miseq|14608 miseq|13565 miseq|13565 miseq|13565 XXXX - XX -- X Musineon divaricatum miseq|14236 NA NA miseq|14236 miseq|14236 miseq|14236 - - - - X ----- Oxyria digyna ncbi|392612348 miseq|13580 miseq|14329 miseq|14329 miseq|14125 miseq|14257 - XXXXXXXX - Oxytropis deflexa miseq|14691 miseq|14691 NA miseq|14678 miseq|14691 miseq|14678 - - - X ------Oxytropis parryi miseq|14695 NA NA miseq|14695 NA miseq|14695 - - - X ------Packera subnuda ncbi|5080722 NA NA miseq|14580 NA miseq|14580 X ---- X ---- Parnassia fimbriata miseq|14370 NA ncbi|7240411 miseq|14370 miseq|14370 miseq|14370 X ------X -- Pedicularis groenlandica ncbi|663082187 miseq|14596 miseq|14410 miseq|14410 miseq|14410 miseq|14410 X ---- X - X -- Penstemon montanus var idahoensis miseq|13570 miseq|13570 miseq|13570 miseq|13570 miseq|13570 miseq|13570 - XX - X ----- Penstemon procerus var formosus ncbi|110666676 miseq|14447 miseq|14447 miseq|14447 miseq|14447 miseq|14447 ------X -- Penstemon sp miseq|14676 miseq|14676 miseq|14676 miseq|14676 NA miseq|14676 - - - X ------Phacelia hastata ncbi|49037339 NA NA miseq|14245 miseq|14245 miseq|14245 - - X ---- X -- Phleum alpinum ncbi|961593651 miseq|14594 miseq|14594 NA miseq|14423 miseq|14423 X ---- X - X -- Phlox diffusa miseq|14624 NA miseq|14624 miseq|14624 miseq|14624 miseq|14624 - - - - - X ---- Phlox pulvinata miseq|14020 miseq|14020 NA miseq|14020 miseq|14254 miseq|14020 X - XXXX - X - X Phyllodoce empetriformis miseq|14021 miseq|14021 miseq|14021 miseq|14021 miseq|14021 miseq|14021 X --- XXXXXX Phyllodoce glanduliflora miseq|14023 miseq|14023 miseq|14023 miseq|14023 miseq|14023 miseq|14023 X ---- XX -- X

Continued on next page Table Appendix S3 – continued from previous page

Species ITS trnTLF rbcL matK atpB ndhF MeadowBraxonCastleD.O. PeakHorstmann Peak LeeHyndmanMount PeakSalzburgerSnowyside Peak CramerPeakThompson Spitzl Peak Peak

Picea engelmannii NA miseq|13571 miseq|13571 ncbi|512745598 miseq|13571 NA - X ------Pinus albicaulis ncbi|6715570 miseq|13573 ncbi|45862048 ncbi|148832373 miseq|14030 NA XX -- X --- XX Poa fendleriana subsp fendleriana miseq|14132 miseq|14132 miseq|14132 NA miseq|14132 miseq|14132 - - X - X --- X - Poa secunda miseq|14007 miseq|13568 NA NA miseq|13568 miseq|13568 XXXX - X - XXX Polemonium viscosum miseq|13554 miseq|13554 miseq|14353 miseq|13554 miseq|14271 miseq|13554 - - XX - X - XXX Polygonum viviparum NA NA NA NA miseq|14573 miseq|14573 X ---- X ---- Potentilla brevifolia miseq|14015 ncbi|337294395 NA miseq|14015 miseq|14015 miseq|14015 X ----- X -- X Potentilla glandulosa miseq|14405 NA NA miseq|14255 miseq|14255 miseq|14255 - - X -- XXXX - Potentilla glandulosa var glandulosa NA NA NA NA NA NA - - - - X ----- Potentilla glaucophylla var glaucophylla miseq|14426 NA miseq|14572 miseq|14426 miseq|14426 miseq|14426 X -- X - X - X - X Ranunculus eschscholtzii ncbi|50811939 NA NA miseq|14239 miseq|14239 miseq|14239 - - X ------Ranunculus eschscholtzii var eschscholtzii miseq|14027 miseq|14027 NA miseq|14027 miseq|14027 miseq|14027 X ------X Ribes lacustre miseq|14436 miseq|14002 miseq|14618 miseq|14002 miseq|14002 miseq|14002 - XX -- X - X -- Salix arctica ncbi|1015860089 miseq|14421 ncbi|667487186 miseq|14421 miseq|14421 miseq|14421 X ---- X - X -- Salix nivalis ncbi|1015860215 miseq|14660 miseq|14660 miseq|14660 NA miseq|14660 X -- X ------38 Saxifraga bronchialis miseq|14006 miseq|14006 NA miseq|14006 miseq|14006 miseq|14006 - X ------Saxifraga ferruginea NA NA NA NA NA NA - - X ------Saxifraga mertensiana ncbi|32395745 miseq|14424 ncbi|459084 miseq|14424 miseq|14570 miseq|14424 X ---- X - X -- Saxifraga oppositifolia miseq|14708 ncbi|14248852 ncbi|459086 miseq|14708 NA NA - - - X ------Sedum debile NA miseq|14272 miseq|14272 miseq|14272 miseq|14272 miseq|14272 X - XX ------Sedum lanceolatum var lanceolatum miseq|13576 miseq|13539 miseq|14137 NA NA miseq|13539 XX - XXXXX - X Selaginella densa ncbi|932248466 NA ncbi|27448379 NA NA NA - - X ----- X - Selaginella watsonii ncbi|932248628 NA ncbi|27448411 NA NA NA ------X -- Senecio fremontii miseq|14005 NA NA miseq|14275 NA miseq|14334 XXXXXX - XXX Senecio residifolius NA NA NA miseq|14579 miseq|14579 miseq|14579 X ---- X ---- Senecio werneriifolius miseq|14672 NA miseq|14671 miseq|14243 miseq|14243 miseq|14243 X - XX ---- X - Sibbaldia procumbens miseq|13574 ncbi|937226908 ncbi|957741012 miseq|14227 miseq|14227 miseq|14227 XXXXXXXX - X Silene acaulis miseq|13555 ncbi|239837528 ncbi|379033973 ncbi|156125845 miseq|13555 miseq|13555 X ------X Smelowskia calycina miseq|13550 miseq|13550 miseq|14465 miseq|13550 miseq|13550 miseq|13550 - - - X - X - XXX Solidago multiradiata miseq|14124 miseq|14124 miseq|14124 miseq|14124 miseq|14419 miseq|14124 X -- XXX - X -- Stenotus acaulis miseq|14278 NA miseq|14704 miseq|14278 NA miseq|14278 X - XX ------Synthyris pinnatifida var cenescens miseq|14028 miseq|14028 miseq|14028 miseq|14028 miseq|14028 miseq|14028 X ------X Taraxacum lyratum NA NA NA miseq|14443 miseq|14443 miseq|14443 - - - - - X - X -- Tonestus lyallii miseq|14362 NA NA miseq|14308 miseq|14362 miseq|14308 - - X -- XX - X - Trisetum spicatum miseq|13552 miseq|13552 miseq|14692 NA miseq|14138 miseq|13552 X - XXXXXXXX Unknown miseq|14706 NA NA miseq|14706 NA miseq|14706 X -- XX -- X -- Veronica wormskjoldii miseq|14587 miseq|14587 miseq|14587 miseq|14587 miseq|14587 miseq|14587 X ---- X ---- Viola adunca miseq|14576 miseq|14576 ncbi|424949998 miseq|14576 miseq|14576 NA X ---- X ---- Table Appendix S4: Illumina paired-end read statistics, and alignment summaries for sequence data generated from the high-throughput approach, sequences retrieved from GenBank, and the combined total dataset for each gene region.

Alignment % Missing Number of Alignment Number of Alignment Number of Length % Missing % Missing Number Data in Primer Number Accessions Length Accessions Length (bp) Accessions (bp) Total Data in Data in of Reads % of Reads High- Gene Region Pair of Raw High- (bp) High- GenBank GenBank Total Data GenBank Total Data After Retained throughput Name Reads throughput throughput Dataset Dataset Data Dataset Alignment Alignment Screen Alignment Dataset Dataset (pre-clean) (pre-clean) Dataset (pre- (cleaned) (cleaned) (cleaned) clean)

ITS ITS 847564 161379 19.04 183 1001 103 644 286 6189 44.06 15.04 25.97 atpB 257 1002 9 1406 265 963 39.86 6.35 36.67 atpB1 76352 2032 2.66 133 566 atpB2 104818 4585 4.37 194 436 matK 303 1424 71 1287 364 1090 37.18 23.83 20.53 matK1 218158 55142 25.28 291 712 matK2 242604 17585 7.25 203 712 ndhF 382 1948 23 2073 391 491 56.18 14.51 0.14 ndhF1 191040 5925 3.1 181 765 ndhF1 231950 42449 18.3 370 492 ndhF1 230590 3551 18.3 81 691 rbcL rbcL 178230 9617 5.4 93 534 47 1308 140 534 0 27.49 2.52 trnTLF 165 2345 84 791 114 561 73.31 26.54 21 trn ab 246874 6749 2.73 107 1110 trn cd 645298 40324 6.25 95 1235 7509 4248 Concatenated 424 8254 121 157 71.21 70.07 39.78 39 (cleaned) (cleaned) Penstemon montanus var idahoensis 14279

Penstemon montanus var idahoensis 14234

Penstemon montanus var idahoensis 13570 Pedicularis groenlandica 14410 Bupleurum americanum 13560 Pedicularis groenlandica 14596

Gentiana calycosa var obtusiloba 14032 Castilleja applegatei 14644 Penstemon procerus var formosus 14447 Gentiana calycosa 14577 Synthyris pinnatifida var cenescens 14028 Castilleja miniata 14586 Castilleja miniata 14369 Lomatium idahoense 14246 Musineon divaricatum 14236 Castilleja cusickii 14238 Lomatium idahoense 14134

Bupleurum americanum 14364 Castilleja miniata 14289 Castilleja rhexiifolia 14406

Castilleja cusickii 14331 Castilleja miniata 14332 Erythranthe tilingii 14574 Castilleja cusickiiCastilleja 14368 cusickii 14296 Erythranthe tilingii 14413 Gentiana calycosa 14414 Chionophila tweedyi 14294 Veronica wormskjoldii 14587 Penstemon sp 14676

Eritrichium nanum 14711 Eritrichium nanum 14713

Eritrichium nanum 14712 Chionophila tweedyi 14013 Chionophila tweedyi 14008

Phlox cf diffusa 14624 Phlox cf pulvinata 14461

Phlox cf pulvinata 14254 ● ●● ●●● Phacelia hastata 14245 ● ● Phlox sp 14606 ● ● ● Chaenactis alpina 13553 ● Chaenactis alpina 14337 ● Chaenactis alpina 14297 ● ● Chaenactis alpina 13579 Chaenactis alpina 14433 ● Chaenactis alpina 14283 Phlox pulvinata 14215 Hieracium triste 14299 Hieracium triste 14017 ● ● ● ● Hulsea algida 14276 Phlox sp 14686 ● Taraxacum lyratum 14615 Hulsea algida 14365 Taraxacum lyratum 14443 Hulsea algida 13545 Phyllodoce empetriformis 14219 Phlox pulvinata 14669 ● Hulsea algida 14321

Crepis nana 14699 Arnica latifolia 14361 ● Hulsea algida 13575 PolemoniumPolemonium viscosumPhlox pulvinata 14353viscosum 14020 14698 Hulsea algida 14459 Arnica latifolia var gracilis 13577 ● ● ●● Hulsea algida 14627 Arnica latifolia 14599 ● ● Hulsea algidaArnica 14222 latifolia 14303

Cirsium subniveum 14288 Polemonium viscosum 14271 ● Cirsium scariosum 14435 ●● Phyllodoce empetriformis 14359 ● Arnica latifolia var gracilis 14128 ● ●● ●● Arnica latifolia 14316 Senecio residifolius 14579 ● ● Arnica mollisArnica 14453 parryi 14240 ● ● ● ● ● ● Arnica chamissonis 14416 Polemonium viscosum 14467 ● ● ● ● Arnica chamissonis 14582 Senecio fremontii 14005 ● Senecio werneriifolius 14671 Polemonium viscosum 14633 ● ● ●● Senecio werneriifolius 14672 ● Senecio fremontii 14334 Senecio● werneriifolius 14338 Polemonium viscosum 13554 ● Senecio fremontii 14707 Senecio werneriifolius● 14243 Senecio fremontiiSenecio 14275 werneriifoliusSenecio werneriifolius 14685 14277 Phyllodoce empetriformis 14291 ● ● Senecio fremontii 14466 ● Phyllodoce empetriformis 14412 ● ● ● Packera subnuda 14580 Collomia debilis 14336 ● ● ●● ● ● ● ● Phyllodoce empetriformisCollomia 14597 debilis 14680 ● ● ● Phyllodoce empetriformis 14021 ● ● Phyllodoce empetriformis 14422 ●

Cassiope mertensianaPhyllodoce var gracilis glanduliflora 14022 14290 Antennaria alpina 14322 Phyllodoce glanduliflora 14581 ● Antennaria alpina 14019 Cassiope mertensiana var gracilis 14646 ● AntennariaAntennariaAntennaria umbrinella corymbosa alpina 13540 14311 14418 Phyllodoce glanduliflora 14023 ● ● Cassiope mertensiana var gracilis 14292 ● ●● ● ● ● Antennaria alpina 14360 Androsace septentrionalis 14464 ● ● ● ● AntennariaAntennaria alpina alpina 14591 14258 ● ● ● ● Antennaria lanata 14312 Androsace septentrionalis 14689 ● ● Eriogonum ovalifolium var depressum 14649 ● Antennaria lanata 14016 ● ● ● ● Antennaria alpina 14661 Eriogonum ovalifolium var depressum 14602 ● ● ●● Antennaria alpina 14684 ● AntennariaAntennaria rosea umbrinella 14273 14446 ● Eriogonum ovalifolium 14327 ● ●● Antennaria umbrinella 14601 ● Antennaria umbrinella 14223 ● ● Antennaria microphylla 14348 ● Antennaria umbrinella 14460 Eriogonum ovalifolium 14244 ● Eriogonum ovalifolium 14280 ● ● Antennaria microphylla 14004 ● ● ● ● Artemisia dracunculus 14284 Eriogonum ovalifolium var depressum 14450 Achillea millefolium var alpicola 14665 Achillea millefolium var alpicola 14136 Eriogonum ovalifolium 14344 ● ● Eriogonum ovalifolium 14349 ● ● Achillea millefolium var alpicola 14287 Eriogonum ovalifolium var depressum 14702 ● Achillea millefolium var alpicola 14431 ● Achillea millefolium var alpicola 14619 Eriogonum ovalifoliumEriogonum var depressumovalifolium 1436613542 ● ● Haplopappus macronema 14274 ● ● Haplopappus macronema 14626 Eriogonum ovalifolium var depressum 13564 ●● ● Haplopappus suffruticosus 14657 Eriogonum ovalifolium var purpureum 14133 ● Ericameria discoidea 14315 ● ● Haplopappus macronema 14342 Eriogonum ovalifolium var depressum 13543 ● Aster foliaceus var apricus 14430 Eriogonum pyrolaefolium var coryphaeum 14126 ● ● Aster occidentalis var occidentalis 14420 Eriogonum flavum var piperi 14654 ● ● ● Aster foliaceus var apricus 14648 ● ● Aster foliaceus var apricus 14583 ●● ● Eriogonum pyrolaefolium var coryphaeum 13567 ● Eriogonum flavum var piperi 14650 Stenotus acaulis 14278 ● ●● Stenotus acaulis 14704 ● Stenotus acaulis 14666 ● ● ● Tonestus lyallii 14308 ● ● Tonestus lyallii 14362 Eriogonum crosbyae 13541● ● ● Tonestus lyallii 14622 Eriogonum crosbyae 14242● ● ● SolidagoSolidago multiradiata multiradiata 14653 14419 Eriogonum crosbyae 14607 ● ● ● Solidago multiradiata 14124 Eriogonum crosbyae 14681 ● Aster alpinus 14609 Eriogonum crosbyae 14350 ● Solidago multiradiata 14595 Oxyria digyna 14445 ● ● Erigeron lonchophyllus 14612 Oxyria digyna 14257 ● Erigeron grandiflorus 14012 ● ● ● Oxyria digyna 14343 ● Erigeron grandiflorus 14310 Oxyria digyna 14125 ● ● Erigeron asperugineus 14247 Oxyria digyna 13580 ● ● Erigeron grandiflorus 14600 ● Erigeron sp 14667 Oxyria digyna 14329 ●● ● Oxyria digyna 14631 ● Erigeron evermannii 13578 ● ● Erigeron evermannii 14340 Bistorta bistortoides 14417 ● Erigeron evermannii 14224 Bistorta bistortoides 14432 ●● ● Erigeron evermannii 14363 Bistorta bistortoides 14584 ● ● Erigeron compositus 14355 Polygonum viviparum 14573 ● ● Erigeron compositus 14009 Aconogonon phytolaccaefolium 14229 Erigeron compositus 14248 ● ● Minuartia obtusiloba 13565 ● Erigeron compositus 14307 ● ● Erigeron compositus 14700 Minuartia obtusiloba 14304 ● Erigeron compositus 14623 Minuartia obtusiloba 14621 ● ● ● Erigeron compositus 13537 ● Erigeron compositus 14452 Minuartia obtusiloba 14705 ● ● ● ● Juniperus communis 14330 Minuartia nuttallii 13544 ● Juniperus communis 14285 ● Abies lasiocarpa 14029 ● Juniperus communis 14031 Minuartia obtusiloba 14249 ● ● ● Abies lasiocarpa 14232 Juniperus communis 14233 ● Abies lasiocarpa 14351 Minuartia nuttallii 14305 ● Picea engelmannii 13571 Minuartia obtusiloba 14608 ● ● Pinus albicaulis 14231 ● ● Pinus albicaulis 14030 Cerastium beeringianum 14687 ● Pinus albicaulis 13573 ● Cerastium arvense 14575Cerastium beeringianum 14628 ● ● Cerastium beeringianum 14451 Cerastium beeringianum 14632 Juncus paryii 14658 ● ● ● ● ● ● ● ● Juncus paryii 14325 Eremogone aculeata 14438 ● ● Juncus paryii 14228 Eremogone congesta 14589Eremogone aculeata 14663 ● Silene acaulis 13555 ● Juncus drummondii 14129 Eremogone congesta 14670 ● Juncus paryiiJuncus 14440 drummondii 14025 ● ● ● ● ● Luzula spicata 14637 Claytonia megarhizaClaytonia 14697 megarhiza 14614 ● Luzula piperi 13572 ● ● ● ● Luzula spicata 14269 Juncus paryii 14263 Claytonia megarhiza 14448 ● ● ● Luzula spicata 13547 Claytonia megarhiza 14293 ● Luzula spicata 14003

Claytonia megarhiza 14267 Carex straminiformis 14468 ● ● ● Carex cf microptera 14638 Claytonia megarhiza 14341 ● Carex straminiformis 14635 Claytonia megarhiza var bellidifolia 14001 ● Carex straminiformis 13548 ● ● ● ● Carex straminiformis 13563 ● ● Claytonia megarhiza var bellidifoliaCistanthe 14034 umbellata 14434 Carex straminiformis 14226 Lewisia pygmaea var pygmara 14592 ●● ● ● ● Carex cf scopulorum 14018 Lewisia pygmaea var pygmara 14010 Carex cf luzulina 14458 ● ● Arabis cf lemmonii 14454 Carex nardina 14026 ● ● Carex nardina 13549 ArabisArabis sp 14455 cf lemmonii 14683 ● Carex nardina 14603 ● ● Arabis cf lemmonii 14266 ● ● Arabis cf lemmonii 14345 ● Arabis cfUnknown lemonii 13546 14706 ● ● ● ● ● Trisetum sp 14295 ● ● Trisetum spicatum 14639 Arabis microphylla 14306 Trisetum spicatumTrisetum 14652 spicatum 13552 ● ● Trisetum spicatum 14463 Arabis microphylla 14011 ● ● Arabis cf lemonii 14668 ● ● Trisetum spicatum 14358 Arabis cf lemonii 14630 ● ● ● ● ● ● Trisetum spicatum 14261 Erysimum asperum 14235 ● ● ● Trisetum spicatum 14317 Erysimum asperum 14302 ● Trisetum spicatum 14138 ● ● Trisetum spicatum 14313 Smelowskia calycina 14469 ● ● Trisetum spicatum 14411 ● ● ● Smelowskia calycina 14465 ● ● ● ● Calamagrostis purpurescens 14319 ● ● CalamagrostisTrisetum purpurescens spicatum 1467714641 Smelowskia calycina 14617 ● Smelowskia calycina 14352 ● Trisetum spicatum 14692 Smelowskia calycina 13550 ● ● ● ● ● ● ● Smelowskia calycina 14693 ● ● ● Anemone drummondii var drummondii 13562 ●● Draba lonchocarpa 14694 ● Ribes lacustre 14002 Anemone drummondii 14682 ● Ribes lacustre 14259 Aquilegia flavescens 14428 Calamagrostis purpurescens 13551 Draba lonchocarpa 14690 ● ● Anemone drummondii 14309 ● ● ● ● ● Ribes lacustre 14618 ● Elymus elymoides 14636 Draba lonchocarpa 14629 ● ●● Heuchera parvifolia var dissecta 14250 ● Draba lonchocarpa 14610 Ribes lacustre 14436 Heuchera parvifolia var dissecta 14346 ● Elymus elymoides 14356 Heuchera parvifolia var dissecta 14216 ● Heuchera parvifolia var dissecta 14320 ● RanunculusRanunculus eschscholtzii eschscholtzii var eschscholtzii 14239 14027 ●● ● ● Elymus elymoides 14439 ● Heuchera parvifolia var dissecta 14625 Heuchera parvifolia var dissecta 14449 ● Draba oligosperma 14604 ● ● ● ● Deschampsia● cespitosa subsp cespitosa 14585 ● ●● ● ● ● ● ● Deschampsia cespitosaElymus subsp elymoides cespitosa 14251 14662 ●● Deschampsia cespitosaElymus subsp elymoides cespitosa 14225 14407 Draba cf lonchocarpa 14268 ● ● Saxifraga mertensiana 14424 ● ● ● Saxifraga mertensiana 14570 Draba oligosperma 14688 ●● ●● Draba cf densifolia 14605 ● ● ● Festuca brachyphylla subsp brachyphylla 14640 Draba oreibata 14335 ● ● ● ● Phleum alpinum 14423 Draba oreibata 14217 ● ● Elymus scribneri 14673 ● ● ● ● Phleum alpinum 14594● Festuca brachyphylla subsp brachyphylla 14262 Draba oligosperma 14703 ● Saxifraga bronchialis 14006 ● Draba cf oreibata 14404 ● ● ● Festuca brachyphylla subsp brachyphylla 14318 Salix arctica 14578 Salix arctica 14421 ● ●● Salix nivalis 14660 ● Poa sp 14590 Festuca brachyphylla subsp brachyphylla 13566 Draba cf densifolia 14241 ● Poa fendleriana subsp fendleriana 14367 Draba cf crassifolia 14218 ● Poa fendleriana subsp fendleriana 14282 Draba cf crassifolia 13569 Epilobium alpinum 14301 ● Poa secunda 14674 Epilobium alpinumEpilobium 14675 alpinum 14409 ●● Poa secunda 14642 Epilobium alpinum 14339 ● Poa secundaPoa 13568 secunda 14462 Epilobium alpinum 14252 ● Poa fendleriana subsp fendleriana 14132 PotentillaPotentilla glandulosa brevifolia 14255 14015 ● ● Poa secunda 14007 Potentilla brevifolia 14328 Ivesia gordonii 14347 Lupinus argenteus var depressus 14286 Ivesia gordonii 13559 Ivesia gordonii 14613 ● Poa secunda 14643 Saxifraga oppositifolia 14708 Epilobium obcordatum 14130 ●● Viola cf adunca 14576 ● Ivesia gordoniiIvesia gordonii14024 14664 Sedum debile 14272 ● Sedum debile 14656 ● ● ● Lupinus argenteus var depressus 14647 Lupinus argenteus var depressus 14408 Poa secunda 14281 Sedum lanceolatum var lanceolatum 14444 Epilobium alpinum var clavatum 14123 Sibbaldia sp 14651 Poa secunda 14357 Sibbaldia sp 14230 Sedum lanceolatum var lanceolatum 13576 PotentillaPotentilla glandulosa glandulosa 14634 14442 Sedum lanceolatum var lanceolatum 13539

Sedum lanceolatum var lanceolatum 14300 Sibbaldia procumbens 14256 Sibbaldia procumbensSibbaldia procumbens 14323 14425

Dasiphora fruticosa 14415 Parnassia fimbriata 14370 Sibbaldia procumbens 14227

Sedum lanceolatum var lanceolatum 14137 Sibbaldia procumbens 13574 Potentilla glandulosa 14314 Sedum lanceolatum var lanceolatum 14611 Potentilla glandulosa 14405

Sedum lanceolatum var lanceolatum 14655 Potentilla glaucophylla var glaucophylla 14572

Potentilla glaucophylla var glaucophylla 14593 Potentilla glaucophylla var glaucophylla 14426 Oxytropis parryi 14695

Oxytropis cf deflexa 14691 Oxytropis cf deflexa 14678

Astragalus alpinus 14427

Astragalus cf eucosmus 14598

Astragalus kentrophyta 14701

Astragalus kentrophyta 14456 Astragalus kentrophyta 14620

● BS = 100 ● BS ≥ 95 ● 75 ≤ BS < 95 ● BS < 75

Figure Appendix S5a: Phylogram for 422 individuals representing 152 unique species of the alpine flora of the Sawtooth National Forest (93% of species collected). Relationships estimated under maximum likelihood from concatenated gene regions (ITS, atpB, matK, ndhF, rbcL, and trnTLF ) of molecular sequence data generated by targeted, high-throughput sequencing. Node values show bootstrap support (BS).

40 Veronica wormskjoldii

Pedicularis groenlandica Veronica paysoni

Erythranthe tilingii Castilleja rhexiifolia

Castilleja miniata

Castilleja cusickii Eritrichium nanum

Polemonium viscosum Phacelia hastata Androsace septentrionalis Phyllodoce empetriformis Phlox pulvinataPhlox diffusa

● Collomia debilis Penstemon procerus Chionophila tweedyi Penstemon montanus Phyllodoce glanduliflora Musineon divaricatumLomatium idahoense ● ● ● ● Bupleurum americanum ● ● ● ● Cirsium scariosum Hieracium tristeAgoseris aurantiaca Cassiope mertensiana ● ● Cirsium subniveum ● Crepis nana Packera subnuda ● ● ● MinuartiaSabulina obtusiloba nuttallii ● ● ● ● ● Arnica mollis ● ● ● ● Hulsea algida ● Cerastium arvense Achillea millefolium Cerastium beeringianum ● ● ● Artemisia dracunculus ● ● Antennaria lanata

Eremogone congesta ● ● ● Antennaria corymbosa ● ● ● Antennaria umbrinella Eremogone aculeata ● ● ● Antennaria microphylla ● ● Antennaria alpina ● ● ● Antennaria rosea Silene acaulis ● Aster alpinus Claytonia megarhiza ● Symphyotrichum foliaceum ● Lewisia pygmaea ● Erigeron compositus ● ● Erigeron lonchophyllus Calyptridium umbellatum ● ● ● Erigeron grandiflorus ● Eriogonum flavum ● ● Ericameria discoidea Eriogonum ovalifolium ● ● Stenotus acaulis ● ● Tonestus lyallii Bistorta vivipara ● ● ● Solidago multiradiata Oxyria digyna ● Cystopteris fragilis

Saxifraga oppositifolia ● ● Cryptogramma crispa ● ● Selaginella densa ● AbiesJuniperus lasiocarpa Selaginellacommunis watsonii Saxifraga bronchialis ● ● ● Pinus albicaulis ● Picea engelmannii ● Saxifraga mertensiana ● Heuchera parvifolia

Micranthes ferrugineaRibes lacustre ●

● ● ● ● Juncus drummondii ● ● Luzula spicata Sedum lanceolatum ● ● Astragalus alpinus ● Luzula piperi ● ● ● ● Carex microptera ● ● Carex straminiformis Astragalus kentrophyta ● ● ● ● ● Carex nardina AstragalusOxytropis eucosmus parryi ● ● ● ● Carex scopulorum ● ● ● ● ElymusCarex elymoides luzulina Oxytropis deflexa Anemone drummondii ● ● Trisetum spicatum Ranunculus eschscholtzii ● Aquilegia flavescens Lupinus argenteus ● Deschampsia cespitosa ● Festuca brachyphylla ● Phleum alpinum Potentilla glaucophylla ● ● ● Potentilla brevifolia ● ● Poa secunda ● Poa fendleriana ● Smelowskia calycina ● ● Erysimum asperum Potentilla gordonii Boechera lemmonii

Salix nivalis Epilobium obcordatum Salix arctica SibbaldiaDasiphora procumbens fruticosa

Viola adunca

Drymocallis glandulosa

Parnassia fimbriataDraba stenoloba

Draba densifolia Draba crassifolia Draba lonchocarpa ● BS = 100 Draba oligosperma ● BS ≥ 95

Boechera microphylla ● 75 ≤ BS < 95 ● BS < 75

Figure Appendix S5b: Phylogram for 121 species of the alpine flora of the Sawtooth Na- tional Forest (74% of species collected). Relationships estimated under maximum likelihood from concatenated gene regions (ITS, atpB, matK, ndhF, rbcL, and trnTLF ) of molecular sequence data retrieved from GenBank. Node values show bootstrap support.

41 Penstemon montanus var idahoensis

Castilleja applegatei

Castilleja cusickii

Pedicularis groenlandica

Castilleja miniata Bupleurum americanum Castilleja rhexiifolia

Musineon divaricatum

Lomatium idahoense

Penstemon procerus var formosus Erythranthe tilingii

Gentiana calycosa var obtusiloba Penstemon sp ● ● ●

Agoseris aurantiaca

Chionophila tweedyi Synthyris pinnatifida var cenescens

● Crepis nana Veronica wormskjoldii ● Stenotus acaulis Gentiana calycosa Tonestus lyallii

Solidago multiradiata Cirsium subniveum Aster alpinus ● Cirsium scariosum ● ● Haplopappus suffruticosus ● Arnica mollis ● ● Ericameria discoidea ● ● ● Aster occidentalis var occidentalis ● Haplopappus macronema ● ● ● Aster foliaceus var apricus ● Erigeron lonchophyllus ● ● ● ● ●● Erigeron grandiflorus ●

● Erigeron asperugineus ● ● ● ● Erigeron evermannii ● Phlox diffusa Phacelia hastata Eritrichium nanum ● Erigeron sp ● ● Erigeron compositusArtemisia dracunculus Phlox pulvinata ● Achillea millefolium var alpicola ● ● Antennaria lanata ● Collomia debilis ● ● Antennaria rosea Polemonium viscosum ● Phyllodoce glanduliflora ● ● Antennaria microphylla ● ●

Phyllodoce empetriformis ● ● Antennaria corymbosa Cassiope mertensiana var gracilis ● Antennaria alpina ● ● Androsace septentrionalis Eremogone aculeata Antennaria umbrinella Senecio fremontii ● ● ● Senecio residifolius Eremogone congesta ● ● Cerastium beeringianum ● ● Senecio werneriifolius Cerastium arvense ● Packera subnuda ● ● ● Minuartia obtusiloba Hieracium triste ● ● ● Minuartia nuttallii ● Taraxacum lyratum

Hulsea algida Silene acaulis ● ● Claytonia megarhiza ● Chaenactis alpina ● Claytonia megarhiza var bellidifolia ● ● Arnica parryi ● ● Lewisia pygmaea var pygmara Arnica chamissonis ● ● Cistanthe umbellata Arnica latifolia var gracilis ●

Eriogonum ovalifolium var depressum ● Arnica latifolia ● ● ● Selaginella densa Selaginella watsonii Eriogonum ovalifolium var purpureum ● ● Juniperus communis● Cystopteris fragilis

Eriogonum flavum var piperi ● Abies lasiocarpa Cryptogramma crispa ● ● ● Picea engelmannii

Eriogonum pyrolaefolium var coryphaeum ● Pinus albicaulis ● Eriogonum ovalifolium

Eriogonum crosbyae ●

● Bistorta bistortoides ● ● Polygonum viviparum

● Juncus drummondii

Aconogonon phytolaccaefolium ● Oxyria digyna ● ● ● Juncus paryii ● Luzula spicata Potentilla brevifolia ● ● Luzula piperi ● Carex nardina Ivesia gordonii ● Potentilla glaucophylla var glaucophylla ● ● ● ● Carex straminiformis Potentilla glandulosa ● Carex microptera Dasiphora fruticosa ● ● Carex luzulina

Sibbaldia procumbens ● ● Carex scopulorum ● ● ● ● ● Trisetum spicatum Astragalus eucosmus ● Calamagrostis purpurescens ● ● Astragalus alpinus ● ● ●

● Poa secunda ● Oxytropis parryi ● Elymus elymoides ● ● Astragalus kentrophyta ● Poa fendleriana subsp fendleriana ● Oxytropis deflexa ● Phleum alpinum ● ● ● ● ●

● Anemone drummondii var drummondii Anemone drummondii Aquilegia flavescens Festuca brachyphylla subsp brachyphylla Elymus scribneri Salix arctica ● Deschampsia cespitosa subsp cespitosa ● Salix nivalis Ranunculus eschscholtzii var eschscholtzii Ranunculus eschscholtzii Ribes lacustre ● ● Heuchera parvifolia var dissecta Lupinus argenteus var depressus ● ● ● ●

Saxifraga bronchialis ● Saxifraga mertensiana Viola adunca Unknown ● Saxifraga oppositifolia ● ● Arabis lemmonii ● ● ●

Parnassia fimbriata

Arabis microphylla

Erysimum asperum

Smelowskia calycina

Epilobium alpinum

Draba oligosperma Draba stenoloba Draba crassifolia ● Draba densifolia Draba oreibata Epilobium obcordatum Draba lonchocarpa

Sedum debile

Sedum lanceolatum var lanceolatum

Epilobium alpinum var clavatum

● BS = 100 ● BS ≥ 95 ● 75 ≤ BS < 95 ● BS < 75

Figure Appendix S5c: Phylogram for 156 species of the alpine flora of the Sawtooth Na- tional Forest (96% of species collected). Relationships estimated under maximum likelihood from concatenated gene regions (ITS, atpB, matK, ndhF, rbcL, and trnTLF ) of the total dataset (combining molecular sequence data from GenBank and targeted high-throughput MiSeq). Node values show bootstrap support.

42 (a) Tracheophyta Asterales Poales Caryophyllales Lamiales Brassicales Ericales

NS NS NS NS NS NS NS 2

1 ●

pool ● 0 All Alpine Talus SES MNTD

−1

−2

(b) Tracheophyta Asterales Poales Caryophyllales Lamiales Brassicales Ericales

NS NS NS NS NS NS NS 2

1

pool 0 All Alpine Talus SES MPD

● −1

−2

Figure Appendix S6: Comparison of phylogenetic community structure between micro- habitats within each summit. Boxplots of standardized effect sizes (SES) for a) mean nearest taxon phylogenetic distance (MNTD) and b) mean pairwise phylogenetic distance (MPD) of all species collected above tree-line (All Alpine) and for species only occurring only on talus slopes (excluding species collected in alpine meadows; Talus). Positive SES values indicate phylogenetic overdispersion (high phylogenetic divergence), negative SES indicate phyloge- netic clustering (low phylogenetic divergence). The significance of a t-test comparing the means of each group is indicated about the box: p < 0.05 * , p < 0.01 **, p < 0.001 ***, NS = not significant. Boxes show first and third quartiles, solid horizontal lines indicate the median, vertical black lines are the range, and dots are points that lie 1.5x the interquartile range above the third quartile or below the first quartile.

43 Table Appendix S7: Phylogenetic α-diversity output statistics. Table summarizes: the number of taxa (N taxa), the observed phylogenetic diversity (obs), the mean phylogenetic diversity from the phylogeny shuffle randomization (rand mean), the standard deviation of phylogenetic diversity from the phylogeny shuffle randomization (rand sd), the rank of the observed metric compared to the metric calculated after the phylogeny shuffle randomization (obs rank), the z-score or standardized effect size (SES) of the observed metric compared to the phylogeny shuffle randomization (obs z) , the P-value (quantile) of observed metric compared to the phylogeny shuffle randomization (obs p = obs rank / runs; runs = 10,000), if the P-value was significant (sig), the phylogenetic diversity metric calculated (metric), the clade the metric was calculated for (clade), the species pool considered (species pool; All Alpine = species collected above treeline, Talus = only species collected on the talus slopes, Meadow = only species collected in alpine meadows), and the dataset that was used (dataset; the total combined high-throughput and GenBank dataset (Total Data), and the GenBank and high-throughput data separately.

summit N taxa obs rand mean rand sd obs rank obs z P-value sig metric clade species pool dataset

Summit N taxa obs rand mean rand sd obs.rank obs.z obs.p sig metric clade Species Pool Dataset Braxon Peak 27 86.5574 122.1410 25.4324 789 -1.3991 0.0789 0 MNTD Tracheophyta All Alpine Total Data Castle Peak 52 100.0094 87.2385 14.0369 8136 0.9098 0.8135 0 MNTD Tracheophyta All Alpine Total Data D.O. Lee Peak 53 68.3731 86.2889 14.0022 1028 -1.2795 0.1028 0 MNTD Tracheophyta All Alpine Total Data Horstmann Peak 36 107.4455 106.0053 20.1690 5329 0.0714 0.5328 0 MNTD Tracheophyta All Alpine Total Data

44 Hyndman Peak 71 62.0548 71.9423 10.1148 1708 -0.9775 0.1708 0 MNTD Tracheophyta All Alpine Total Data Mount Cramer 38 100.2822 103.4071 18.9456 4438 -0.1649 0.4438 0 MNTD Tracheophyta All Alpine Total Data Salzburger Spitzl 64 91.6268 76.7050 11.3450 9041 1.3153 0.9040 0 MNTD Tracheophyta All Alpine Total Data Snowyside Peak 35 110.1064 108.0524 20.1943 5505 0.1017 0.5504 0 MNTD Tracheophyta All Alpine Total Data Thompson Peak 47 69.0620 92.2332 15.6979 724 -1.4761 0.0724 0 MNTD Tracheophyta All Alpine Total Data Braxon Peak 7 23.3300 40.3249 11.6573 767 -1.4579 0.0767 0 MNTD Asterales All Alpine Total Data Castle Peak 17 17.8039 25.6841 5.7081 869 -1.3805 0.0869 0 MNTD Asterales All Alpine Total Data D.O. Lee Peak 11 38.4006 32.9989 8.5182 7340 0.6341 0.7339 0 MNTD Asterales All Alpine Total Data Horstmann Peak 7 43.6359 40.3437 11.6485 5985.5 0.2826 0.5985 0 MNTD Asterales All Alpine Total Data Hyndman Peak 19 25.1219 23.8015 5.0358 5969 0.2622 0.5968 0 MNTD Asterales All Alpine Total Data Mount Cramer 10 26.5297 34.5516 9.1643 1943 -0.8753 0.1943 0 MNTD Asterales All Alpine Total Data Salzburger Spitzl 14 37.6515 28.8486 6.8095 8998 1.2927 0.8997 0 MNTD Asterales All Alpine Total Data Snowyside Peak 11 26.1216 32.9245 8.4440 2113 -0.8056 0.2113 0 MNTD Asterales All Alpine Total Data Thompson Peak 9 16.2544 36.3383 10.0409 210 -2.0002 0.0210 1 MNTD Asterales All Alpine Total Data Braxon Peak 5 77.6119 77.3420 19.2816 4530.5 0.0140 0.4530 0 MNTD Caryophyllales All Alpine Total Data Castle Peak 5 91.1386 77.3017 19.3665 7815 0.7145 0.7814 0 MNTD Caryophyllales All Alpine Total Data D.O. Lee Peak 9 39.5578 53.5236 11.3634 1109 -1.2290 0.1109 0 MNTD Caryophyllales All Alpine Total Data Horstmann Peak 4 44.3298 89.5173 23.4153 387 -1.9298 0.0387 1 MNTD Caryophyllales All Alpine Total Data Hyndman Peak 11 51.0798 47.1077 9.1913 6562 0.4322 0.6561 0 MNTD Caryophyllales All Alpine Total Data Mount Cramer 5 65.9393 77.4090 19.3955 2603 -0.5914 0.2603 0 MNTD Caryophyllales All Alpine Total Data Salzburger Spitzl 8 59.3772 57.5083 12.7551 5501 0.1465 0.5500 0 MNTD Caryophyllales All Alpine Total Data Snowyside Peak 4 97.6535 89.4094 23.3414 6164 0.3532 0.6163 0 MNTD Caryophyllales All Alpine Total Data Thompson Peak 7 36.7313 62.7294 14.2441 387.5 -1.8252 0.0387 1 MNTD Caryophyllales All Alpine Total Data Braxon Peak 5 68.7059 103.7413 35.3418 1938 -0.9913 0.1938 0 MNTD Poales All Alpine Total Data Castle Peak 7 64.9294 76.1975 23.0365 3263 -0.4891 0.3263 0 MNTD Poales All Alpine Total Data D.O. Lee Peak 7 95.3460 75.9219 23.0941 8151 0.8411 0.8150 0 MNTD Poales All Alpine Total Data Horstmann Peak 6 109.9849 87.7383 28.2679 7919 0.7870 0.7918 0 MNTD Poales All Alpine Total Data Hyndman Peak 12 57.0876 46.9271 8.5004 8895.5 1.1953 0.8895 0 MNTD Poales All Alpine Total Data Mount Cramer 6 84.1038 87.8356 28.1512 4337.5 -0.1326 0.4337 0 MNTD Poales All Alpine Total Data Salzburger Spitzl 9 76.7658 60.1013 15.6260 8421.5 1.0665 0.8421 0 MNTD Poales All Alpine Total Data Snowyside Peak 5 104.0140 102.9684 35.5151 4621 0.0294 0.4621 0 MNTD Poales All Alpine Total Data Thompson Peak 8 70.9567 66.8450 19.0356 5787 0.2160 0.5786 0 MNTD Poales All Alpine Total Data Braxon Peak 2 33.5857 59.6275 24.3239 1717 -1.0706 0.1717 0 MNTD Lamiales All Alpine Total Data

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Castle Peak 3 24.7917 48.6228 18.8724 902.5 -1.2627 0.0902 0 MNTD Lamiales All Alpine Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine Total Data Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine Total Data Hyndman Peak 4 54.2947 41.3508 14.8692 8391 0.8705 0.8390 0 MNTD Lamiales All Alpine Total Data Mount Cramer 3 24.7933 48.9690 18.7088 1231.5 -1.2922 0.1231 0 MNTD Lamiales All Alpine Total Data Salzburger Spitzl 6 29.8539 32.0950 8.7979 4007 -0.2547 0.4007 0 MNTD Lamiales All Alpine Total Data Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine Total Data Thompson Peak 2 81.0373 59.9608 24.0027 9219 0.8781 0.9218 0 MNTD Lamiales All Alpine Total Data Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales All Alpine Total Data Castle Peak 5 11.4179 24.4770 6.6743 373 -1.9566 0.0373 1 MNTD Brassicales All Alpine Total Data D.O. Lee Peak 4 20.3685 27.7305 8.3590 992 -0.8807 0.0992 0 MNTD Brassicales All Alpine Total Data Horstmann Peak 3 52.4590 32.6624 11.2534 9286.5 1.7592 0.9286 0 MNTD Brassicales All Alpine Total Data Hyndman Peak 5 19.4269 24.5078 6.6027 1625.5 -0.7695 0.1625 0 MNTD Brassicales All Alpine Total Data Mount Cramer 2 34.8714 40.9479 16.4368 3301.5 -0.3697 0.3301 0 MNTD Brassicales All Alpine Total Data Salzburger Spitzl 3 33.3666 32.7770 11.3992 6150 0.0517 0.6149 0 MNTD Brassicales All Alpine Total Data Snowyside Peak 3 33.3666 32.8434 11.3039 6127 0.0463 0.6126 0 MNTD Brassicales All Alpine Total Data Thompson Peak 3 10.6927 32.7751 11.3123 42.5 -1.9521 0.0042 1 MNTD Brassicales All Alpine Total Data Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales All Alpine Total Data Castle Peak 2 65.8227 126.9026 52.6942 2491.5 -1.1591 0.2491 0 MNTD Ericales All Alpine Total Data

45 D.O. Lee Peak 5 103.0133 69.7212 21.2586 9482.5 1.5661 0.9482 0 MNTD Ericales All Alpine Total Data Horstmann Peak 2 157.2220 127.0840 52.3412 3945 0.5758 0.3945 0 MNTD Ericales All Alpine Total Data Hyndman Peak 6 50.0698 61.5201 15.7865 3392.5 -0.7253 0.3392 0 MNTD Ericales All Alpine Total Data Mount Cramer 3 27.6861 100.1949 36.5981 98.5 -1.9812 0.0098 1 MNTD Ericales All Alpine Total Data Salzburger Spitzl 4 114.8218 82.3652 26.9297 9116 1.2052 0.9115 0 MNTD Ericales All Alpine Total Data Snowyside Peak 3 96.2892 100.1555 36.5644 6266 -0.1057 0.6265 0 MNTD Ericales All Alpine Total Data Thompson Peak 5 42.9407 69.6875 21.3917 1430.5 -1.2503 0.1430 0 MNTD Ericales All Alpine Total Data Braxon Peak 27 316.6929 292.9847 37.4447 7348 0.6332 0.7347 0 MPD Tracheophyta All Alpine Total Data Castle Peak 52 302.9660 293.3287 24.1402 6625 0.3992 0.6624 0 MPD Tracheophyta All Alpine Total Data D.O. Lee Peak 53 255.3348 293.4840 24.4150 584 -1.5625 0.0584 0 MPD Tracheophyta All Alpine Total Data Horstmann Peak 36 378.3130 293.5490 31.5564 9952 2.6861 0.9951 1 MPD Tracheophyta All Alpine Total Data Hyndman Peak 71 271.4435 293.3197 18.9084 1246 -1.1570 0.1246 0 MPD Tracheophyta All Alpine Total Data Mount Cramer 38 308.6313 293.6992 30.3086 6954 0.4927 0.6953 0 MPD Tracheophyta All Alpine Total Data Salzburger Spitzl 64 288.3109 293.3296 20.5370 4127 -0.2444 0.4127 0 MPD Tracheophyta All Alpine Total Data Snowyside Peak 35 344.7016 293.5177 32.1667 9411 1.5912 0.9410 0 MPD Tracheophyta All Alpine Total Data Thompson Peak 47 310.7068 293.2304 26.1756 7455 0.6677 0.7454 0 MPD Tracheophyta All Alpine Total Data Braxon Peak 7 44.1707 62.6975 10.0547 283 -1.8426 0.0283 1 MPD Asterales All Alpine Total Data Castle Peak 17 59.0142 62.6616 5.2253 2437 -0.6980 0.2437 0 MPD Asterales All Alpine Total Data D.O. Lee Peak 11 66.2239 62.7997 7.4381 6616 0.4604 0.6615 0 MPD Asterales All Alpine Total Data Horstmann Peak 7 53.2957 62.7517 10.0830 2041.5 -0.9378 0.2041 0 MPD Asterales All Alpine Total Data Hyndman Peak 19 58.3148 62.7110 4.7844 1846 -0.9189 0.1846 0 MPD Asterales All Alpine Total Data Mount Cramer 10 52.5405 62.7140 7.9914 1185 -1.2731 0.1185 0 MPD Asterales All Alpine Total Data Salzburger Spitzl 14 64.4652 62.7353 6.2674 5886 0.2760 0.5885 0 MPD Asterales All Alpine Total Data Snowyside Peak 11 52.2882 62.6793 7.5231 974 -1.3812 0.0974 0 MPD Asterales All Alpine Total Data Thompson Peak 9 43.0991 62.8542 8.5748 71 -2.3038 0.0071 1 MPD Asterales All Alpine Total Data Braxon Peak 5 142.1737 135.3598 14.1178 5975.5 0.4826 0.5975 0 MPD Caryophyllales All Alpine Total Data Castle Peak 5 145.5554 135.1921 14.0579 7870 0.7372 0.7869 0 MPD Caryophyllales All Alpine Total Data D.O. Lee Peak 9 133.2565 135.3161 5.8101 2739 -0.3545 0.2739 0 MPD Caryophyllales All Alpine Total Data Horstmann Peak 4 83.2995 135.4158 19.1588 337 -2.7202 0.0337 1 MPD Caryophyllales All Alpine Total Data Hyndman Peak 11 140.3036 135.2025 4.2057 9719 1.2129 0.9718 1 MPD Caryophyllales All Alpine Total Data Mount Cramer 5 142.1380 135.2765 14.0323 5937.5 0.4890 0.5937 0 MPD Caryophyllales All Alpine Total Data Salzburger Spitzl 8 140.9297 135.3192 7.0612 7992 0.7946 0.7991 0 MPD Caryophyllales All Alpine Total Data Snowyside Peak 4 132.7980 135.1946 19.1331 3258.5 -0.1253 0.3258 0 MPD Caryophyllales All Alpine Total Data

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Thompson Peak 7 132.2956 135.2579 8.7271 2646.5 -0.3394 0.2646 0 MPD Caryophyllales All Alpine Total Data Braxon Peak 5 238.2204 207.9428 36.6389 8169.5 0.8264 0.8169 0 MPD Poales All Alpine Total Data Castle Peak 7 190.3901 207.7379 25.5982 2017 -0.6777 0.2017 0 MPD Poales All Alpine Total Data D.O. Lee Peak 7 207.6834 207.8919 25.3576 4558.5 -0.0082 0.4558 0 MPD Poales All Alpine Total Data Horstmann Peak 6 237.2635 207.9785 29.8940 8444 0.9796 0.8443 0 MPD Poales All Alpine Total Data Hyndman Peak 12 199.7521 207.7143 13.1417 2203.5 -0.6059 0.2203 0 MPD Poales All Alpine Total Data Mount Cramer 6 222.6390 207.7173 30.1589 6729 0.4948 0.6728 0 MPD Poales All Alpine Total Data Salzburger Spitzl 9 185.1299 208.3151 19.1388 1032 -1.2114 0.1032 0 MPD Poales All Alpine Total Data Snowyside Peak 5 175.4207 207.7333 36.0305 2347.5 -0.8968 0.2347 0 MPD Poales All Alpine Total Data Thompson Peak 8 226.8871 208.1022 22.0051 8333 0.8537 0.8332 0 MPD Poales All Alpine Total Data Braxon Peak 2 33.5857 59.8304 24.3659 1674.5 -1.0771 0.1674 0 MPD Lamiales All Alpine Total Data Castle Peak 3 46.3723 59.5801 15.1803 990.5 -0.8701 0.0990 0 MPD Lamiales All Alpine Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine Total Data Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine Total Data Hyndman Peak 4 67.8945 59.8262 10.7085 6704.5 0.7534 0.6704 0 MPD Lamiales All Alpine Total Data Mount Cramer 3 46.3731 59.8760 14.7747 1368.5 -0.9139 0.1368 0 MPD Lamiales All Alpine Total Data Salzburger Spitzl 6 47.7813 59.7502 6.9414 800.5 -1.7243 0.0800 0 MPD Lamiales All Alpine Total Data Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine Total Data Thompson Peak 2 81.0373 59.7569 24.1409 9245 0.8815 0.9244 0 MPD Lamiales All Alpine Total Data

46 Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales All Alpine Total Data Castle Peak 5 35.9696 40.8521 5.2411 1355.5 -0.9316 0.1355 0 MPD Brassicales All Alpine Total Data D.O. Lee Peak 4 40.6775 40.7980 7.0417 3918 -0.0171 0.3918 0 MPD Brassicales All Alpine Total Data Horstmann Peak 3 54.0858 40.8576 9.8249 9250 1.3464 0.9249 0 MPD Brassicales All Alpine Total Data Hyndman Peak 5 37.7714 40.7785 5.3608 2302 -0.5609 0.2302 0 MPD Brassicales All Alpine Total Data Mount Cramer 2 34.8714 40.9474 16.3660 3311 -0.3713 0.3311 0 MPD Brassicales All Alpine Total Data Salzburger Spitzl 3 42.0994 40.9938 9.7109 5364 0.1138 0.5363 0 MPD Brassicales All Alpine Total Data Snowyside Peak 3 42.0994 40.9047 9.8307 5388 0.1215 0.5387 0 MPD Brassicales All Alpine Total Data Thompson Peak 3 17.6633 40.8029 10.0065 914.5 -2.3125 0.0914 0 MPD Brassicales All Alpine Total Data Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales All Alpine Total Data Castle Peak 2 65.8227 126.5664 52.9559 2522 -1.1471 0.2522 0 MPD Ericales All Alpine Total Data D.O. Lee Peak 5 134.5792 127.0491 13.5198 6421 0.5570 0.6420 0 MPD Ericales All Alpine Total Data Horstmann Peak 2 157.2220 126.5495 52.6401 3978 0.5827 0.3978 0 MPD Ericales All Alpine Total Data Hyndman Peak 6 130.6110 127.0338 9.3703 4526 0.3818 0.4526 0 MPD Ericales All Alpine Total Data Mount Cramer 3 45.5664 127.0052 29.5130 97 -2.7594 0.0097 1 MPD Ericales All Alpine Total Data Salzburger Spitzl 4 148.5877 126.8715 19.5273 9008.5 1.1121 0.9008 0 MPD Ericales All Alpine Total Data Snowyside Peak 3 126.7556 126.4155 29.8360 6284 0.0114 0.6283 0 MPD Ericales All Alpine Total Data Thompson Peak 5 114.5854 126.7738 13.5893 2165 -0.8969 0.2165 0 MPD Ericales All Alpine Total Data Braxon Peak 27 86.5574 122.5023 25.3115 764 -1.4201 0.0764 0 MNTD Tracheophyta Talus Total Data Castle Peak 52 100.0094 83.9525 13.5977 8766 1.1809 0.8765 0 MNTD Tracheophyta Talus Total Data D.O. Lee Peak 32 81.4550 113.0550 22.0609 747 -1.4324 0.0747 0 MNTD Tracheophyta Talus Total Data Horstmann Peak 36 107.4455 105.9831 19.9110 5311 0.0734 0.5310 0 MNTD Tracheophyta Talus Total Data Hyndman Peak 43 61.3895 95.0900 16.6179 189 -2.0280 0.0189 1 MNTD Tracheophyta Talus Total Data Mount Cramer 38 100.2822 102.2834 18.6645 4521 -0.1072 0.4521 0 MNTD Tracheophyta Talus Total Data Salzburger Spitzl 44 109.7043 93.9102 16.1599 8296 0.9774 0.8295 0 MNTD Tracheophyta Talus Total Data Snowyside Peak 35 110.1064 107.5211 20.3970 5509 0.1268 0.5508 0 MNTD Tracheophyta Talus Total Data Thompson Peak 17 98.9153 150.8029 36.4729 705 -1.4226 0.0705 0 MNTD Tracheophyta Talus Total Data Braxon Peak 7 23.3300 40.4171 11.7637 755 -1.4525 0.0755 0 MNTD Asterales Talus Total Data Castle Peak 17 17.8039 25.2778 5.5840 957 -1.3384 0.0957 0 MNTD Asterales Talus Total Data D.O. Lee Peak 6 55.9373 42.7263 12.9507 8240 1.0201 0.8239 0 MNTD Asterales Talus Total Data Horstmann Peak 7 43.6359 40.2175 11.6742 6027.5 0.2928 0.6027 0 MNTD Asterales Talus Total Data Hyndman Peak 12 33.8140 31.2445 7.9542 6300 0.3230 0.6299 0 MNTD Asterales Talus Total Data Mount Cramer 10 26.5297 34.4488 9.1853 1982.5 -0.8622 0.1982 0 MNTD Asterales Talus Total Data

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Salzburger Spitzl 10 52.4218 34.3535 9.2391 9750 1.9556 0.9749 1 MNTD Asterales Talus Total Data Snowyside Peak 11 26.1216 32.7806 8.5845 2270 -0.7757 0.2270 0 MNTD Asterales Talus Total Data Thompson Peak 4 34.8152 48.6673 15.5623 2215 -0.8901 0.2215 0 MNTD Asterales Talus Total Data Braxon Peak 5 77.6119 76.0925 18.7168 4343.5 0.0812 0.4343 0 MNTD Caryophyllales Talus Total Data Castle Peak 5 91.1386 76.1529 18.7202 8216 0.8005 0.8215 0 MNTD Caryophyllales Talus Total Data D.O. Lee Peak 6 51.2929 67.4102 15.9638 1573 -1.0096 0.1573 0 MNTD Caryophyllales Talus Total Data Horstmann Peak 4 44.3298 88.5882 23.3222 400 -1.8977 0.0400 1 MNTD Caryophyllales Talus Total Data Hyndman Peak 6 86.9281 67.1465 15.7320 9263.5 1.2574 0.9263 0 MNTD Caryophyllales Talus Total Data Mount Cramer 5 65.9393 75.7944 18.7138 2641.5 -0.5266 0.2641 0 MNTD Caryophyllales Talus Total Data Salzburger Spitzl 7 72.0465 60.9709 14.0113 7629.5 0.7905 0.7629 0 MNTD Caryophyllales Talus Total Data Snowyside Peak 4 97.6535 88.2184 23.3961 6333.5 0.4033 0.6333 0 MNTD Caryophyllales Talus Total Data Thompson Peak 3 95.9433 106.9997 31.2060 3667 -0.3543 0.3667 0 MNTD Caryophyllales Talus Total Data Braxon Peak 5 68.7059 108.1105 34.4015 1554 -1.1454 0.1554 0 MNTD Poales Talus Total Data Castle Peak 7 64.9294 76.8555 22.0346 3028.5 -0.5412 0.3028 0 MNTD Poales Talus Total Data D.O. Lee Peak 4 135.0204 131.8647 45.7550 4637.5 0.0690 0.4637 0 MNTD Poales Talus Total Data Horstmann Peak 6 109.9849 90.2895 27.2403 7558.5 0.7230 0.7558 0 MNTD Poales Talus Total Data Hyndman Peak 9 80.9515 58.6616 14.1213 9342 1.5785 0.9341 0 MNTD Poales Talus Total Data Mount Cramer 6 84.1038 89.9989 27.3547 4204 -0.2155 0.4204 0 MNTD Poales Talus Total Data Salzburger Spitzl 7 94.1309 77.2411 22.4139 7741 0.7535 0.7740 0 MNTD Poales Talus Total Data

47 Snowyside Peak 5 104.0140 107.7356 34.6329 4188.5 -0.1075 0.4188 0 MNTD Poales Talus Total Data Thompson Peak 5 110.2723 108.2476 34.5301 4754.5 0.0586 0.4754 0 MNTD Poales Talus Total Data Braxon Peak 2 33.5857 47.6257 24.9738 3333.5 -0.5622 0.3333 0 MNTD Lamiales Talus Total Data Castle Peak 3 24.7917 33.2883 10.4663 1132 -0.8118 0.1132 0 MNTD Lamiales Talus Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Mount Cramer 3 24.7933 33.1984 10.4174 3737.5 -0.8068 0.3737 0 MNTD Lamiales Talus Total Data Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Thompson Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus Total Data Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Talus Total Data Castle Peak 5 11.4179 24.4422 6.6770 356.5 -1.9506 0.0356 1 MNTD Brassicales Talus Total Data D.O. Lee Peak 4 20.3685 27.5540 8.3355 1032 -0.8620 0.1032 0 MNTD Brassicales Talus Total Data Horstmann Peak 3 52.4590 32.7510 11.4096 9220 1.7273 0.9219 0 MNTD Brassicales Talus Total Data Hyndman Peak 5 19.4269 24.3551 6.6756 1708.5 -0.7382 0.1708 0 MNTD Brassicales Talus Total Data Mount Cramer 2 34.8714 40.5452 16.6724 3413 -0.3403 0.3413 0 MNTD Brassicales Talus Total Data Salzburger Spitzl 3 33.3666 32.7728 11.2784 6126 0.0526 0.6125 0 MNTD Brassicales Talus Total Data Snowyside Peak 3 33.3666 32.8352 11.3394 6100 0.0469 0.6099 0 MNTD Brassicales Talus Total Data Thompson Peak 2 24.6339 40.7392 16.5636 2577 -0.9723 0.2577 0 MNTD Brassicales Talus Total Data Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales Talus Total Data Castle Peak 2 65.8227 126.6135 52.3824 2512 -1.1605 0.2512 0 MNTD Ericales Talus Total Data D.O. Lee Peak 3 100.6884 99.5962 36.5081 7822 0.0299 0.7821 0 MNTD Ericales Talus Total Data Horstmann Peak 2 157.2220 126.4990 52.8405 3968.5 0.5814 0.3968 0 MNTD Ericales Talus Total Data Hyndman Peak 4 70.2018 82.3778 26.7949 4417.5 -0.4544 0.4417 0 MNTD Ericales Talus Total Data Mount Cramer 3 27.6861 100.4302 36.6459 91 -1.9851 0.0091 1 MNTD Ericales Talus Total Data Salzburger Spitzl 3 100.6884 100.2743 36.3036 7511 0.0114 0.7510 0 MNTD Ericales Talus Total Data Snowyside Peak 3 96.2892 99.8257 36.6097 6214 -0.0966 0.6213 0 MNTD Ericales Talus Total Data Thompson Peak 1 NA NA NA NA NA NA NA MNTD Ericales Talus Total Data Braxon Peak 27 316.6929 304.9397 39.4155 6204 0.2982 0.6203 0 MPD Tracheophyta Talus Total Data Castle Peak 52 302.9660 305.0908 24.4869 4657 -0.0868 0.4657 0 MPD Tracheophyta Talus Total Data D.O. Lee Peak 32 253.9212 304.0223 34.9568 729 -1.4332 0.0729 0 MPD Tracheophyta Talus Total Data Horstmann Peak 36 378.3130 305.2031 32.8256 9848 2.2272 0.9847 1 MPD Tracheophyta Talus Total Data

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Hyndman Peak 43 264.1531 305.2978 28.5966 799 -1.4388 0.0799 0 MPD Tracheophyta Talus Total Data Mount Cramer 38 308.6313 304.9071 31.6156 5496 0.1178 0.5495 0 MPD Tracheophyta Talus Total Data Salzburger Spitzl 44 309.0480 305.0093 27.9717 5607 0.1444 0.5606 0 MPD Tracheophyta Talus Total Data Snowyside Peak 35 344.7016 304.3461 33.4204 8818 1.2075 0.8817 0 MPD Tracheophyta Talus Total Data Thompson Peak 17 286.2809 304.1200 51.8938 3895 -0.3438 0.3895 0 MPD Tracheophyta Talus Total Data Braxon Peak 7 44.1707 63.0099 9.8173 238.5 -1.9190 0.0238 1 MPD Asterales Talus Total Data Castle Peak 17 59.0142 62.9916 4.7923 2098 -0.8300 0.2098 0 MPD Asterales Talus Total Data D.O. Lee Peak 6 70.6641 62.9351 10.9404 7434 0.7065 0.7433 0 MPD Asterales Talus Total Data Horstmann Peak 7 53.2957 62.9585 9.7542 1920.5 -0.9906 0.1920 0 MPD Asterales Talus Total Data Hyndman Peak 12 52.9129 63.0313 6.5644 776 -1.5414 0.0776 0 MPD Asterales Talus Total Data Mount Cramer 10 52.5405 62.8296 7.5568 1018.5 -1.3616 0.1018 0 MPD Asterales Talus Total Data Salzburger Spitzl 10 67.7457 62.9666 7.5062 7259 0.6367 0.7258 0 MPD Asterales Talus Total Data Snowyside Peak 11 52.2882 63.0067 7.1454 796 -1.5001 0.0796 0 MPD Asterales Talus Total Data Thompson Peak 4 45.5010 63.0161 14.3013 1231 -1.2247 0.1231 0 MPD Asterales Talus Total Data Braxon Peak 5 142.1737 135.3649 14.1391 5666 0.4816 0.5665 0 MPD Caryophyllales Talus Total Data Castle Peak 5 145.5554 135.2213 14.2362 7548 0.7259 0.7547 0 MPD Caryophyllales Talus Total Data D.O. Lee Peak 6 137.2839 135.3431 11.0280 4800 0.1760 0.4800 0 MPD Caryophyllales Talus Total Data Horstmann Peak 4 83.2995 135.1731 19.5234 385.5 -2.6570 0.0385 1 MPD Caryophyllales Talus Total Data Hyndman Peak 6 147.2172 135.1832 11.0014 9496 1.0939 0.9495 0 MPD Caryophyllales Talus Total Data

48 Mount Cramer 5 142.1380 135.2913 13.9393 5583.5 0.4912 0.5583 0 MPD Caryophyllales Talus Total Data Salzburger Spitzl 7 144.8287 135.2967 8.7129 9493.5 1.0940 0.9493 1 MPD Caryophyllales Talus Total Data Snowyside Peak 4 132.7980 135.1863 19.5373 3566.5 -0.1222 0.3566 0 MPD Caryophyllales Talus Total Data Thompson Peak 3 137.4326 135.3377 28.6129 4075 0.0732 0.4075 0 MPD Caryophyllales Talus Total Data Braxon Peak 5 238.2204 216.7909 31.1525 7638 0.6879 0.7637 0 MPD Poales Talus Total Data Castle Peak 7 190.3901 217.0288 20.5166 1012.5 -1.2984 0.1012 0 MPD Poales Talus Total Data D.O. Lee Peak 4 171.8157 216.7318 41.4065 1707 -1.0848 0.1707 0 MPD Poales Talus Total Data Horstmann Peak 6 237.2635 217.2336 25.0074 7546.5 0.8010 0.7546 0 MPD Poales Talus Total Data Hyndman Peak 9 197.5723 217.0541 14.1504 1361.5 -1.3768 0.1361 0 MPD Poales Talus Total Data Mount Cramer 6 222.6390 217.7864 24.2713 5446 0.1999 0.5445 0 MPD Poales Talus Total Data Salzburger Spitzl 7 207.4809 217.4897 19.9328 2499 -0.5021 0.2499 0 MPD Poales Talus Total Data Snowyside Peak 5 175.4207 217.0243 31.2362 1372.5 -1.3319 0.1372 0 MPD Poales Talus Total Data Thompson Peak 5 231.3710 217.1562 31.2830 6187.5 0.4544 0.6187 0 MPD Poales Talus Total Data Braxon Peak 2 33.5857 47.7050 24.9806 3340 -0.5652 0.3340 0 MPD Lamiales Talus Total Data Castle Peak 3 46.3723 47.6833 11.1616 1746 -0.1175 0.1746 0 MPD Lamiales Talus Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Hyndman Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Mount Cramer 3 46.3731 47.6095 11.4227 4269.5 -0.1082 0.4269 0 MPD Lamiales Talus Total Data Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Thompson Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus Total Data Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales Talus Total Data Castle Peak 5 35.9696 40.7946 5.2816 1337.5 -0.9136 0.1337 0 MPD Brassicales Talus Total Data D.O. Lee Peak 4 40.6775 40.8046 6.9813 3924 -0.0182 0.3924 0 MPD Brassicales Talus Total Data Horstmann Peak 3 54.0858 40.6881 9.8851 9254.5 1.3553 0.9254 0 MPD Brassicales Talus Total Data Hyndman Peak 5 37.7714 40.8542 5.2749 2275 -0.5844 0.2275 0 MPD Brassicales Talus Total Data Mount Cramer 2 34.8714 40.8662 16.4289 3324.5 -0.3649 0.3324 0 MPD Brassicales Talus Total Data Salzburger Spitzl 3 42.0994 40.8683 9.6998 5502.5 0.1269 0.5502 0 MPD Brassicales Talus Total Data Snowyside Peak 3 42.0994 40.8792 9.7577 5435 0.1250 0.5434 0 MPD Brassicales Talus Total Data Thompson Peak 2 24.6339 40.9155 16.4734 2530 -0.9884 0.2530 0 MPD Brassicales Talus Total Data Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales Talus Total Data Castle Peak 2 65.8227 127.6011 52.0765 2478 -1.1863 0.2478 0 MPD Ericales Talus Total Data

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

D.O. Lee Peak 3 135.5541 126.9128 29.3161 7769.5 0.2948 0.7769 0 MPD Ericales Talus Total Data Horstmann Peak 2 157.2220 127.7449 52.0443 3877 0.5664 0.3877 0 MPD Ericales Talus Total Data Hyndman Peak 4 110.8646 127.1019 19.3044 2944.5 -0.8411 0.2944 0 MPD Ericales Talus Total Data Mount Cramer 3 45.5664 127.1323 29.3159 86 -2.7823 0.0086 1 MPD Ericales Talus Total Data Salzburger Spitzl 3 135.5541 126.7400 29.3374 7501.5 0.3004 0.7501 0 MPD Ericales Talus Total Data Snowyside Peak 3 126.7556 126.8355 29.0540 6324.5 -0.0027 0.6324 0 MPD Ericales Talus Total Data Thompson Peak 1 NA NA NA NA NA NA NA MPD Ericales Talus Total Data D.O. Lee Peak 28 81.9859 121.4458 24.3254 510 -1.6222 0.0510 1 MNTD Tracheophyta Meadow Total Data Hyndman Peak 28 107.0242 121.7504 24.1632 2846 -0.6094 0.2846 0 MNTD Tracheophyta Meadow Total Data Salzburger Spitzl 23 105.9720 132.0032 27.8471 1805 -0.9348 0.1805 0 MNTD Tracheophyta Meadow Total Data Thompson Peak 32 97.7722 114.5648 21.4528 2255 -0.7828 0.2255 0 MNTD Tracheophyta Meadow Total Data D.O. Lee Peak 8 43.0542 38.0459 9.7949 6825 0.5113 0.6824 0 MNTD Asterales Meadow Total Data Hyndman Peak 7 55.1346 40.0812 10.5902 9112.5 1.4215 0.9112 0 MNTD Asterales Meadow Total Data Salzburger Spitzl 4 55.0347 47.6788 13.4056 7081 0.5487 0.7080 0 MNTD Asterales Meadow Total Data Thompson Peak 5 30.5267 44.7945 12.4576 1285 -1.1453 0.1285 0 MNTD Asterales Meadow Total Data D.O. Lee Peak 4 26.0775 94.7105 23.3578 127.5 -2.9383 0.0127 1 MNTD Caryophyllales Meadow Total Data Hyndman Peak 5 65.0260 83.2045 20.8983 1740.5 -0.8699 0.1740 0 MNTD Caryophyllales Meadow Total Data Salzburger Spitzl 2 178.9219 136.9825 48.1105 9289.5 0.8717 0.9289 0 MNTD Caryophyllales Meadow Total Data Thompson Peak 4 57.9255 94.7308 23.5143 718.5 -1.5652 0.0718 0 MNTD Caryophyllales Meadow Total Data

49 D.O. Lee Peak 4 191.0075 127.2181 46.9162 8839 1.3596 0.8838 0 MNTD Poales Meadow Total Data Hyndman Peak 3 130.6680 162.6835 68.3730 3973 -0.4682 0.3973 0 MNTD Poales Meadow Total Data Salzburger Spitzl 3 63.8216 162.0691 67.8712 300.5 -1.4476 0.0300 1 MNTD Poales Meadow Total Data Thompson Peak 5 126.3671 101.3818 32.2204 7830 0.7754 0.7829 0 MNTD Poales Meadow Total Data D.O. Lee Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow Total Data Hyndman Peak 4 54.2947 42.9726 14.8423 7364.5 0.7628 0.7364 0 MNTD Lamiales Meadow Total Data Salzburger Spitzl 5 22.2342 38.1916 10.5259 437.5 -1.5160 0.0437 1 MNTD Lamiales Meadow Total Data Thompson Peak 2 81.0373 61.6076 24.9961 8560 0.7773 0.8559 0 MNTD Lamiales Meadow Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow Total Data Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow Total Data Salzburger Spitzl 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow Total Data Thompson Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow Total Data D.O. Lee Peak 2 157.2220 100.6219 58.7058 9196 0.9641 0.9195 0 MNTD Ericales Meadow Total Data Hyndman Peak 2 9.8058 101.6442 58.7246 839 -1.5639 0.0839 0 MNTD Ericales Meadow Total Data Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Ericales Meadow Total Data Thompson Peak 4 60.0701 60.0701 0.0000 5001 NA 0.5000 0 MNTD Ericales Meadow Total Data D.O. Lee Peak 28 253.6980 292.5120 31.1445 1102 -1.2463 0.1102 0 MPD Tracheophyta Meadow Total Data Hyndman Peak 28 281.1127 292.4758 31.1862 3655 -0.3644 0.3655 0 MPD Tracheophyta Meadow Total Data Salzburger Spitzl 23 249.9491 292.5694 36.4616 1274 -1.1689 0.1274 0 MPD Tracheophyta Meadow Total Data Thompson Peak 32 330.7541 292.9464 28.1795 9046 1.3417 0.9045 0 MPD Tracheophyta Meadow Total Data D.O. Lee Peak 8 61.4092 58.9291 6.7327 6225 0.3684 0.6224 0 MPD Asterales Meadow Total Data Hyndman Peak 7 68.6105 59.0792 7.5164 8521 1.2681 0.8520 0 MPD Asterales Meadow Total Data Salzburger Spitzl 4 62.4066 59.2747 11.8266 6730.5 0.2648 0.6730 0 MPD Asterales Meadow Total Data Thompson Peak 5 44.6742 59.0879 9.7990 597 -1.4709 0.0597 0 MPD Asterales Meadow Total Data D.O. Lee Peak 4 127.9738 137.2731 16.6033 2508 -0.5601 0.2508 0 MPD Caryophyllales Meadow Total Data Hyndman Peak 5 141.9097 137.4386 11.2582 6118.5 0.3971 0.6118 0 MPD Caryophyllales Meadow Total Data Salzburger Spitzl 2 178.9219 137.6071 47.9424 9249.5 0.8618 0.9249 0 MPD Caryophyllales Meadow Total Data Thompson Peak 4 100.6421 137.4904 16.4012 434 -2.2467 0.0434 1 MPD Caryophyllales Meadow Total Data D.O. Lee Peak 4 252.1068 216.9202 38.9589 7883 0.9032 0.7882 0 MPD Poales Meadow Total Data Hyndman Peak 3 219.5930 216.7382 57.7118 5024 0.0495 0.5023 0 MPD Poales Meadow Total Data Salzburger Spitzl 3 85.9003 216.7016 57.9906 301.5 -2.2556 0.0301 1 MPD Poales Meadow Total Data Thompson Peak 5 204.3458 216.3103 28.4361 2432 -0.4208 0.2432 0 MPD Poales Meadow Total Data D.O. Lee Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow Total Data

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Hyndman Peak 4 67.8945 61.3238 11.1435 6241.5 0.5896 0.6241 0 MPD Lamiales Meadow Total Data Salzburger Spitzl 5 37.6956 61.5645 8.1247 87.5 -2.9378 0.0087 1 MPD Lamiales Meadow Total Data Thompson Peak 2 81.0373 61.1890 25.1731 8584.5 0.7885 0.8584 0 MPD Lamiales Meadow Total Data D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow Total Data Hyndman Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow Total Data Salzburger Spitzl 0 NA NA NA NA NA NA NA MPD Brassicales Meadow Total Data Thompson Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow Total Data D.O. Lee Peak 2 157.2220 101.8642 58.3855 9157 0.9481 0.9156 0 MPD Ericales Meadow Total Data Hyndman Peak 2 9.8058 100.5415 58.7463 853.5 -1.5445 0.0853 0 MPD Ericales Meadow Total Data Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Ericales Meadow Total Data Thompson Peak 4 101.3942 101.3942 0.0000 5001 NA 0.5000 0 MPD Ericales Meadow Total Data Braxon Peak 28 92.2324 98.0828 19.3217 4034 -0.3028 0.4034 0 MNTD Tracheophyta All Alpine High-throughput Castle Peak 46 95.5452 73.9803 12.3988 9622 1.7393 0.9621 1 MNTD Tracheophyta All Alpine High-throughput D.O. Lee Peak 61 65.7717 61.7735 9.2711 6550 0.4312 0.6549 0 MNTD Tracheophyta All Alpine High-throughput Horstmann Peak 33 99.2278 90.1109 16.7197 6988 0.5453 0.6987 0 MNTD Tracheophyta All Alpine High-throughput Hyndman Peak 71 54.0931 55.6058 7.9510 4258 -0.1903 0.4258 0 MNTD Tracheophyta All Alpine High-throughput Mount Cramer 39 90.0818 81.7674 14.2299 7122 0.5843 0.7121 0 MNTD Tracheophyta All Alpine High-throughput Salzburger Spitzl 63 73.5680 60.3334 9.0532 9285 1.4619 0.9284 0 MNTD Tracheophyta All Alpine High-throughput Snowyside Peak 35 82.6457 86.8525 15.8086 4092 -0.2661 0.4092 0 MNTD Tracheophyta All Alpine High-throughput

50 Thompson Peak 43 81.5655 77.1032 13.0749 6265 0.3413 0.6264 0 MNTD Tracheophyta All Alpine High-throughput Braxon Peak 7 38.8754 37.2900 12.3728 5689 0.1281 0.5688 0 MNTD Asterales All Alpine High-throughput Castle Peak 15 25.1760 21.5354 5.7573 7305 0.6324 0.7304 0 MNTD Asterales All Alpine High-throughput D.O. Lee Peak 6 48.1181 41.0674 14.0682 6970.5 0.5012 0.6970 0 MNTD Asterales All Alpine High-throughput Horstmann Peak 6 49.5881 41.3030 14.0744 7240 0.5887 0.7239 0 MNTD Asterales All Alpine High-throughput Hyndman Peak 11 39.2233 26.9134 7.9248 9333 1.5533 0.9332 0 MNTD Asterales All Alpine High-throughput Mount Cramer 12 24.6058 25.3656 7.2888 4721 -0.1042 0.4721 0 MNTD Asterales All Alpine High-throughput Salzburger Spitzl 11 48.3808 26.8800 7.8912 9960 2.7247 0.9959 1 MNTD Asterales All Alpine High-throughput Snowyside Peak 12 20.2424 25.1650 7.2271 2599 -0.6811 0.2599 0 MNTD Asterales All Alpine High-throughput Thompson Peak 4 63.1948 52.7466 19.1932 7454.5 0.5444 0.7454 0 MNTD Asterales All Alpine High-throughput Braxon Peak 5 114.1355 83.5367 27.7009 8772.5 1.1046 0.8772 0 MNTD Caryophyllales All Alpine High-throughput Castle Peak 6 88.3799 72.8873 23.7874 7039.5 0.6513 0.7039 0 MNTD Caryophyllales All Alpine High-throughput D.O. Lee Peak 5 99.5390 83.1044 27.8662 7373 0.5898 0.7372 0 MNTD Caryophyllales All Alpine High-throughput Horstmann Peak 4 75.5540 96.7225 34.7383 2678 -0.6094 0.2678 0 MNTD Caryophyllales All Alpine High-throughput Hyndman Peak 8 68.8961 59.4228 18.5527 6873 0.5106 0.6872 0 MNTD Caryophyllales All Alpine High-throughput Mount Cramer 5 87.0982 82.6626 27.7240 5152.5 0.1600 0.5152 0 MNTD Caryophyllales All Alpine High-throughput Salzburger Spitzl 7 102.4763 65.4057 20.8575 9738 1.7773 0.9737 1 MNTD Caryophyllales All Alpine High-throughput Snowyside Peak 6 75.7762 73.3399 23.4897 5447.5 0.1037 0.5447 0 MNTD Caryophyllales All Alpine High-throughput Thompson Peak 4 90.4532 97.2218 34.5486 4343.5 -0.1959 0.4343 0 MNTD Caryophyllales All Alpine High-throughput Braxon Peak 6 52.1428 79.8988 27.5569 1949 -1.0072 0.1949 0 MNTD Poales All Alpine High-throughput Castle Peak 7 87.7510 72.1219 23.9797 7270 0.6518 0.7269 0 MNTD Poales All Alpine High-throughput D.O. Lee Peak 4 40.0774 101.5056 40.6251 665.5 -1.5121 0.0665 0 MNTD Poales All Alpine High-throughput Horstmann Peak 6 99.8668 79.7628 27.6497 7407 0.7271 0.7406 0 MNTD Poales All Alpine High-throughput Hyndman Peak 10 67.6954 56.9920 16.5054 7365 0.6485 0.7364 0 MNTD Poales All Alpine High-throughput Mount Cramer 6 96.4026 80.0427 27.7971 7038 0.5885 0.7037 0 MNTD Poales All Alpine High-throughput Salzburger Spitzl 6 99.9402 80.2466 27.4600 7388 0.7172 0.7387 0 MNTD Poales All Alpine High-throughput Snowyside Peak 4 53.6209 101.6936 40.3336 1542.5 -1.1919 0.1542 0 MNTD Poales All Alpine High-throughput Thompson Peak 5 99.4675 89.4307 32.5583 6089 0.3083 0.6088 0 MNTD Poales All Alpine High-throughput Braxon Peak 2 44.3366 55.1151 26.3884 4648 -0.4085 0.4648 0 MNTD Lamiales All Alpine High-throughput Castle Peak 2 81.0373 54.7590 26.7078 6002.5 0.9839 0.6002 0 MNTD Lamiales All Alpine High-throughput D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Mount Cramer 3 33.3314 40.9726 12.9599 3460.5 -0.5896 0.3460 0 MNTD Lamiales All Alpine High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput Thompson Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine High-throughput Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales All Alpine High-throughput Castle Peak 3 41.3406 44.9517 13.1999 3263.5 -0.2736 0.3263 0 MNTD Brassicales All Alpine High-throughput D.O. Lee Peak 6 31.2655 30.8581 7.6997 5169 0.0529 0.5168 0 MNTD Brassicales All Alpine High-throughput Horstmann Peak 3 62.1172 44.7334 13.2513 9491.5 1.3119 0.9491 0 MNTD Brassicales All Alpine High-throughput Hyndman Peak 6 30.3024 30.7999 7.6746 4794 -0.0648 0.4794 0 MNTD Brassicales All Alpine High-throughput Mount Cramer 2 41.7034 54.2516 18.9511 2713 -0.6621 0.2713 0 MNTD Brassicales All Alpine High-throughput Salzburger Spitzl 4 8.3683 38.4489 10.4438 53 -2.8802 0.0053 1 MNTD Brassicales All Alpine High-throughput Snowyside Peak 4 38.5979 38.3117 10.3472 4720.5 0.0277 0.4720 0 MNTD Brassicales All Alpine High-throughput Thompson Peak 2 55.8390 54.3194 18.7859 3156 0.0809 0.3156 0 MNTD Brassicales All Alpine High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales All Alpine High-throughput Castle Peak 2 74.0450 127.2650 72.5559 3674 -0.7335 0.3674 0 MNTD Ericales All Alpine High-throughput D.O. Lee Peak 4 98.1373 79.1655 31.8870 7746.5 0.5950 0.7746 0 MNTD Ericales All Alpine High-throughput Horstmann Peak 2 193.2182 127.8395 72.1566 7584 0.9061 0.7583 0 MNTD Ericales All Alpine High-throughput Hyndman Peak 3 41.0503 95.8947 45.4198 1316 -1.2075 0.1316 0 MNTD Ericales All Alpine High-throughput Mount Cramer 3 57.9679 95.9124 45.3037 2227.5 -0.8376 0.2227 0 MNTD Ericales All Alpine High-throughput

51 Salzburger Spitzl 3 116.2154 94.5473 45.3076 8406 0.4782 0.8405 0 MNTD Ericales All Alpine High-throughput Snowyside Peak 3 103.7221 95.1455 45.7547 5629.5 0.1874 0.5629 0 MNTD Ericales All Alpine High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MNTD Ericales All Alpine High-throughput Braxon Peak 28 333.9598 278.8261 29.4296 9588 1.8734 0.9587 1 MPD Tracheophyta All Alpine High-throughput Castle Peak 46 272.7615 278.5058 22.5312 4213 -0.2549 0.4213 0 MPD Tracheophyta All Alpine High-throughput D.O. Lee Peak 61 247.9840 278.8046 19.2137 476 -1.6041 0.0476 1 MPD Tracheophyta All Alpine High-throughput Horstmann Peak 33 345.4227 279.1474 27.0376 9872 2.4512 0.9871 1 MPD Tracheophyta All Alpine High-throughput Hyndman Peak 71 261.5748 278.7064 17.2211 1614 -0.9948 0.1614 0 MPD Tracheophyta All Alpine High-throughput Mount Cramer 39 277.4975 278.8986 24.7554 5040 -0.0566 0.5039 0 MPD Tracheophyta All Alpine High-throughput Salzburger Spitzl 63 256.4486 279.0595 18.8101 1153 -1.2021 0.1153 0 MPD Tracheophyta All Alpine High-throughput Snowyside Peak 35 264.9202 278.5580 25.7494 3211 -0.5296 0.3211 0 MPD Tracheophyta All Alpine High-throughput Thompson Peak 43 328.2761 278.4193 23.3160 9773 2.1383 0.9772 1 MPD Tracheophyta All Alpine High-throughput Braxon Peak 7 76.0905 73.1047 7.3318 6367 0.4072 0.6366 0 MPD Asterales All Alpine High-throughput Castle Peak 15 78.1244 73.1982 3.9568 9082 1.2450 0.9081 0 MPD Asterales All Alpine High-throughput D.O. Lee Peak 6 77.6675 73.1005 8.3587 6987.5 0.5464 0.6987 0 MPD Asterales All Alpine High-throughput Horstmann Peak 6 78.8814 72.9942 8.5395 7526.5 0.6894 0.7526 0 MPD Asterales All Alpine High-throughput Hyndman Peak 11 70.4029 73.0889 5.0896 2642 -0.5277 0.2642 0 MPD Asterales All Alpine High-throughput Mount Cramer 12 74.5922 73.0710 4.7290 6003 0.3217 0.6002 0 MPD Asterales All Alpine High-throughput Salzburger Spitzl 11 83.8274 73.1370 5.1320 9974 2.0831 0.9973 1 MPD Asterales All Alpine High-throughput Snowyside Peak 12 72.0003 73.1303 4.6578 3693 -0.2426 0.3693 0 MPD Asterales All Alpine High-throughput Thompson Peak 4 80.2588 72.9831 12.4826 7264.5 0.5829 0.7264 0 MPD Asterales All Alpine High-throughput Braxon Peak 5 173.4505 152.3438 25.9122 8057.5 0.8145 0.8057 0 MPD Caryophyllales All Alpine High-throughput Castle Peak 6 156.2918 152.0351 22.1262 4494.5 0.1924 0.4494 0 MPD Caryophyllales All Alpine High-throughput D.O. Lee Peak 5 171.2946 152.1609 25.8803 7604 0.7393 0.7603 0 MPD Caryophyllales All Alpine High-throughput Horstmann Peak 4 105.9498 152.0779 32.1464 1059 -1.4349 0.1059 0 MPD Caryophyllales All Alpine High-throughput Hyndman Peak 8 168.9566 152.1421 17.3471 8772 0.9693 0.8771 0 MPD Caryophyllales All Alpine High-throughput Mount Cramer 5 174.3274 152.2091 25.9769 8605 0.8515 0.8604 0 MPD Caryophyllales All Alpine High-throughput Salzburger Spitzl 7 179.2144 152.1242 18.9536 9987 1.4293 0.9986 1 MPD Caryophyllales All Alpine High-throughput Snowyside Peak 6 120.8835 152.0794 22.1054 764.5 -1.4112 0.0764 0 MPD Caryophyllales All Alpine High-throughput Thompson Peak 4 132.5751 151.9442 32.0248 2242 -0.6048 0.2242 0 MPD Caryophyllales All Alpine High-throughput Braxon Peak 6 190.8280 159.8447 38.1324 7958.5 0.8125 0.7958 0 MPD Poales All Alpine High-throughput Castle Peak 7 167.9490 159.4514 34.1540 5407 0.2488 0.5406 0 MPD Poales All Alpine High-throughput D.O. Lee Peak 4 71.9904 160.3985 50.2950 736 -1.7578 0.0736 0 MPD Poales All Alpine High-throughput

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Horstmann Peak 6 204.4259 158.9811 38.0950 8910 1.1929 0.8909 0 MPD Poales All Alpine High-throughput Hyndman Peak 10 160.2096 159.2397 27.1341 4580 0.0357 0.4580 0 MPD Poales All Alpine High-throughput Mount Cramer 6 140.6756 159.8455 38.1513 3400 -0.5025 0.3400 0 MPD Poales All Alpine High-throughput Salzburger Spitzl 6 189.3423 159.6164 38.1717 7872 0.7787 0.7871 0 MPD Poales All Alpine High-throughput Snowyside Peak 4 72.2582 159.0497 51.0733 849 -1.6994 0.0849 0 MPD Poales All Alpine High-throughput Thompson Peak 5 198.7438 159.3187 42.9853 8367.5 0.9172 0.8367 0 MPD Poales All Alpine High-throughput Braxon Peak 2 44.3366 55.1189 26.6183 4591 -0.4051 0.4591 0 MPD Lamiales All Alpine High-throughput Castle Peak 2 81.0373 55.1568 26.5180 5969 0.9760 0.5968 0 MPD Lamiales All Alpine High-throughput D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Mount Cramer 3 57.1844 54.8109 14.7621 4662 0.1608 0.4662 0 MPD Lamiales All Alpine High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Thompson Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine High-throughput Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales All Alpine High-throughput Castle Peak 3 55.0177 54.4631 10.6699 3688.5 0.0520 0.3688 0 MPD Brassicales All Alpine High-throughput D.O. Lee Peak 6 52.4067 54.3125 4.5129 2849.5 -0.4223 0.2849 0 MPD Brassicales All Alpine High-throughput Horstmann Peak 3 65.4061 54.1782 10.8251 9626.5 1.0372 0.9626 1 MPD Brassicales All Alpine High-throughput

52 Hyndman Peak 6 49.3870 54.3136 4.5124 1136 -1.0918 0.1136 0 MPD Brassicales All Alpine High-throughput Mount Cramer 2 41.7034 54.3124 18.9108 2700 -0.6668 0.2700 0 MPD Brassicales All Alpine High-throughput Salzburger Spitzl 4 40.0154 54.2759 7.4561 499 -1.9126 0.0499 0 MPD Brassicales All Alpine High-throughput Snowyside Peak 4 58.6626 54.1957 7.5650 6756.5 0.5905 0.6756 0 MPD Brassicales All Alpine High-throughput Thompson Peak 2 55.8390 54.2062 18.8917 3226 0.0864 0.3226 0 MPD Brassicales All Alpine High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales All Alpine High-throughput Castle Peak 2 74.0450 128.7993 72.2213 3615.5 -0.7581 0.3615 0 MPD Ericales All Alpine High-throughput D.O. Lee Peak 4 134.7888 127.4520 37.6379 5990 0.1949 0.5989 0 MPD Ericales All Alpine High-throughput Horstmann Peak 2 193.2182 126.9564 72.2949 7603.5 0.9165 0.7603 0 MPD Ericales All Alpine High-throughput Hyndman Peak 3 57.5476 127.2473 49.0722 1386.5 -1.4204 0.1386 0 MPD Ericales All Alpine High-throughput Mount Cramer 3 77.7694 128.2116 48.3063 2744 -1.0442 0.2744 0 MPD Ericales All Alpine High-throughput Salzburger Spitzl 3 158.3859 128.0429 48.0696 8186.5 0.6312 0.8186 0 MPD Ericales All Alpine High-throughput Snowyside Peak 3 148.4702 127.4410 48.4550 5630.5 0.4340 0.5630 0 MPD Ericales All Alpine High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MPD Ericales All Alpine High-throughput Braxon Peak 28 92.2324 93.6463 19.0520 4834 -0.0742 0.4834 0 MNTD Tracheophyta Talus High-throughput Castle Peak 46 95.5452 69.8696 12.0449 9874 2.1317 0.9873 1 MNTD Tracheophyta Talus High-throughput D.O. Lee Peak 35 74.7068 82.9686 15.6496 3160 -0.5279 0.3160 0 MNTD Tracheophyta Talus High-throughput Horstmann Peak 33 99.2278 85.6198 16.3041 7850 0.8346 0.7849 0 MNTD Tracheophyta Talus High-throughput Hyndman Peak 45 58.7872 71.1721 12.3788 1695 -1.0005 0.1695 0 MNTD Tracheophyta Talus High-throughput Mount Cramer 39 90.0818 77.4921 14.0397 8057 0.8967 0.8056 0 MNTD Tracheophyta Talus High-throughput Salzburger Spitzl 40 84.5303 76.4260 13.6801 7098 0.5924 0.7097 0 MNTD Tracheophyta Talus High-throughput Snowyside Peak 35 82.6457 83.0073 15.4685 4977 -0.0234 0.4977 0 MNTD Tracheophyta Talus High-throughput Thompson Peak 17 97.7170 121.4783 28.0482 2071 -0.8472 0.2071 0 MNTD Tracheophyta Talus High-throughput Braxon Peak 7 38.8754 36.9050 12.2192 5780 0.1613 0.5779 0 MNTD Asterales Talus High-throughput Castle Peak 15 25.1760 21.4948 5.7613 7390 0.6390 0.7389 0 MNTD Asterales Talus High-throughput D.O. Lee Peak 6 48.1181 41.2530 13.9886 6896 0.4908 0.6895 0 MNTD Asterales Talus High-throughput Horstmann Peak 6 49.5881 41.3525 13.8776 7285 0.5934 0.7284 0 MNTD Asterales Talus High-throughput Hyndman Peak 11 39.2233 27.0624 7.9452 9322 1.5306 0.9321 0 MNTD Asterales Talus High-throughput Mount Cramer 12 24.6058 25.3752 7.2052 4698 -0.1068 0.4698 0 MNTD Asterales Talus High-throughput Salzburger Spitzl 11 48.3808 27.0408 8.0220 9955 2.6602 0.9954 1 MNTD Asterales Talus High-throughput Snowyside Peak 12 20.2424 25.2073 7.2240 2604 -0.6873 0.2604 0 MNTD Asterales Talus High-throughput Thompson Peak 4 63.1948 52.3854 19.3829 7470.5 0.5577 0.7470 0 MNTD Asterales Talus High-throughput Braxon Peak 5 114.1355 82.7757 28.1606 8838.5 1.1136 0.8838 0 MNTD Caryophyllales Talus High-throughput

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Castle Peak 6 88.3799 73.5121 23.8258 7003 0.6240 0.7002 0 MNTD Caryophyllales Talus High-throughput D.O. Lee Peak 5 99.5390 82.3731 27.7688 7411.5 0.6182 0.7411 0 MNTD Caryophyllales Talus High-throughput Horstmann Peak 4 75.5540 96.8889 34.7777 2623.5 -0.6135 0.2623 0 MNTD Caryophyllales Talus High-throughput Hyndman Peak 8 68.8961 59.8068 18.6492 6758 0.4874 0.6757 0 MNTD Caryophyllales Talus High-throughput Mount Cramer 5 87.0982 82.4392 27.7992 5287.5 0.1676 0.5287 0 MNTD Caryophyllales Talus High-throughput Salzburger Spitzl 7 102.4763 65.3623 20.7555 9741 1.7881 0.9740 1 MNTD Caryophyllales Talus High-throughput Snowyside Peak 6 75.7762 72.9115 23.7438 5514 0.1206 0.5513 0 MNTD Caryophyllales Talus High-throughput Thompson Peak 4 90.4532 96.8871 34.0871 4455.5 -0.1887 0.4455 0 MNTD Caryophyllales Talus High-throughput Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Talus High-throughput Castle Peak 3 41.3406 45.0991 13.1960 3233 -0.2848 0.3233 0 MNTD Brassicales Talus High-throughput D.O. Lee Peak 6 31.2655 30.6552 7.7471 5248 0.0788 0.5247 0 MNTD Brassicales Talus High-throughput Horstmann Peak 3 62.1172 44.7007 13.3784 9494.5 1.3018 0.9494 1 MNTD Brassicales Talus High-throughput Hyndman Peak 6 30.3024 30.6400 7.6773 4877 -0.0440 0.4877 0 MNTD Brassicales Talus High-throughput Mount Cramer 2 41.7034 54.0213 19.1281 2805.5 -0.6440 0.2805 0 MNTD Brassicales Talus High-throughput Salzburger Spitzl 4 8.3683 38.3188 10.2852 42 -2.9120 0.0042 1 MNTD Brassicales Talus High-throughput Snowyside Peak 4 38.5979 38.4957 10.3338 4619.5 0.0099 0.4619 0 MNTD Brassicales Talus High-throughput Thompson Peak 2 55.8390 54.3744 18.8941 3137 0.0775 0.3137 0 MNTD Brassicales Talus High-throughput Braxon Peak 2 44.3366 55.0353 26.5174 4640.5 -0.4035 0.4640 0 MNTD Lamiales Talus High-throughput Castle Peak 2 81.0373 54.7253 26.7958 5982 0.9819 0.5981 0 MNTD Lamiales Talus High-throughput

53 D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Mount Cramer 3 33.3314 40.9174 13.0162 3486.5 -0.5828 0.3486 0 MNTD Lamiales Talus High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Thompson Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus High-throughput Braxon Peak 6 52.1428 80.0825 27.5115 1896 -1.0156 0.1896 0 MNTD Poales Talus High-throughput Castle Peak 7 87.7510 72.8508 23.7845 7184 0.6265 0.7183 0 MNTD Poales Talus High-throughput D.O. Lee Peak 4 40.0774 100.7594 40.1901 687 -1.5099 0.0687 0 MNTD Poales Talus High-throughput Horstmann Peak 6 99.8668 79.9833 27.8741 7314.5 0.7133 0.7314 0 MNTD Poales Talus High-throughput Hyndman Peak 10 67.6954 56.7649 16.4843 7384 0.6631 0.7383 0 MNTD Poales Talus High-throughput Mount Cramer 6 96.4026 79.9073 27.7512 7031.5 0.5944 0.7031 0 MNTD Poales Talus High-throughput Salzburger Spitzl 6 99.9402 80.2555 27.6585 7384.5 0.7117 0.7384 0 MNTD Poales Talus High-throughput Snowyside Peak 4 53.6209 101.2899 40.4566 1579 -1.1783 0.1579 0 MNTD Poales Talus High-throughput Thompson Peak 5 99.4675 88.3773 32.4490 6288 0.3418 0.6287 0 MNTD Poales Talus High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales Talus High-throughput Castle Peak 2 74.0450 127.8125 72.1427 3631 -0.7453 0.3631 0 MNTD Ericales Talus High-throughput D.O. Lee Peak 4 98.1373 78.7756 32.0238 7786.5 0.6046 0.7786 0 MNTD Ericales Talus High-throughput Horstmann Peak 2 193.2182 127.2458 72.3911 7564.5 0.9113 0.7564 0 MNTD Ericales Talus High-throughput Hyndman Peak 3 41.0503 95.5526 45.4634 1338.5 -1.1988 0.1338 0 MNTD Ericales Talus High-throughput Mount Cramer 3 57.9679 94.6499 45.3577 2301.5 -0.8087 0.2301 0 MNTD Ericales Talus High-throughput Salzburger Spitzl 3 116.2154 95.0876 45.4946 8322.5 0.4644 0.8322 0 MNTD Ericales Talus High-throughput Snowyside Peak 3 103.7221 95.5034 45.2798 5576.5 0.1815 0.5576 0 MNTD Ericales Talus High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MNTD Ericales Talus High-throughput Braxon Peak 28 333.9598 279.4575 28.9425 9602 1.8831 0.9601 1 MPD Tracheophyta Talus High-throughput Castle Peak 46 272.7615 279.4691 21.7333 3997 -0.3086 0.3997 0 MPD Tracheophyta Talus High-throughput D.O. Lee Peak 35 250.3696 279.1904 25.1524 1220 -1.1458 0.1220 0 MPD Tracheophyta Talus High-throughput Horstmann Peak 33 345.4227 279.7990 26.3064 9896 2.4946 0.9895 1 MPD Tracheophyta Talus High-throughput Hyndman Peak 45 268.5919 279.4831 21.8834 3246 -0.4977 0.3246 0 MPD Tracheophyta Talus High-throughput Mount Cramer 39 277.4975 279.8305 23.9532 4838 -0.0974 0.4838 0 MPD Tracheophyta Talus High-throughput Salzburger Spitzl 40 258.8195 279.4850 23.6449 1960 -0.8740 0.1960 0 MPD Tracheophyta Talus High-throughput Snowyside Peak 35 264.9202 279.7811 25.2619 3010 -0.5883 0.3010 0 MPD Tracheophyta Talus High-throughput

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Thompson Peak 17 284.2254 279.8702 37.9255 6123 0.1148 0.6122 0 MPD Tracheophyta Talus High-throughput Braxon Peak 7 76.0905 73.1801 7.4589 6293 0.3902 0.6292 0 MPD Asterales Talus High-throughput Castle Peak 15 78.1244 73.1055 4.0011 9130 1.2544 0.9129 0 MPD Asterales Talus High-throughput D.O. Lee Peak 6 77.6675 73.1077 8.3928 6958.5 0.5433 0.6958 0 MPD Asterales Talus High-throughput Horstmann Peak 6 78.8814 73.1710 8.5027 7464 0.6716 0.7463 0 MPD Asterales Talus High-throughput Hyndman Peak 11 70.4029 73.0841 5.0611 2628 -0.5297 0.2628 0 MPD Asterales Talus High-throughput Mount Cramer 12 74.5922 73.1552 4.7096 5972 0.3051 0.5971 0 MPD Asterales Talus High-throughput Salzburger Spitzl 11 83.8274 73.1640 5.1055 9969 2.0886 0.9968 1 MPD Asterales Talus High-throughput Snowyside Peak 12 72.0003 73.0678 4.7599 3780 -0.2243 0.3780 0 MPD Asterales Talus High-throughput Thompson Peak 4 80.2588 73.2255 12.2225 7198.5 0.5754 0.7198 0 MPD Asterales Talus High-throughput Braxon Peak 5 173.4505 152.2163 25.8459 8102 0.8216 0.8101 0 MPD Caryophyllales Talus High-throughput Castle Peak 6 156.2918 152.0322 22.2895 4436.5 0.1911 0.4436 0 MPD Caryophyllales Talus High-throughput D.O. Lee Peak 5 171.2946 151.6874 26.0616 7677.5 0.7523 0.7677 0 MPD Caryophyllales Talus High-throughput Horstmann Peak 4 105.9498 152.1231 31.7594 1016.5 -1.4538 0.1016 0 MPD Caryophyllales Talus High-throughput Hyndman Peak 8 168.9566 151.8082 17.4391 8836 0.9833 0.8835 0 MPD Caryophyllales Talus High-throughput Mount Cramer 5 174.3274 152.2975 25.6505 8654 0.8589 0.8653 0 MPD Caryophyllales Talus High-throughput Salzburger Spitzl 7 179.2144 151.7921 19.5522 9994 1.4025 0.9993 1 MPD Caryophyllales Talus High-throughput Snowyside Peak 6 120.8835 152.1354 21.9888 749.5 -1.4213 0.0749 0 MPD Caryophyllales Talus High-throughput Thompson Peak 4 132.5751 151.6210 32.2062 2260 -0.5914 0.2260 0 MPD Caryophyllales Talus High-throughput

54 Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales Talus High-throughput Castle Peak 3 55.0177 54.3597 10.6751 3714 0.0616 0.3714 0 MPD Brassicales Talus High-throughput D.O. Lee Peak 6 52.4067 54.2154 4.6089 2892 -0.3924 0.2892 0 MPD Brassicales Talus High-throughput Horstmann Peak 3 65.4061 54.1817 10.7479 9596 1.0443 0.9595 1 MPD Brassicales Talus High-throughput Hyndman Peak 6 49.3870 54.2540 4.5667 1176 -1.0657 0.1176 0 MPD Brassicales Talus High-throughput Mount Cramer 2 41.7034 54.3605 18.8257 2703.5 -0.6723 0.2703 0 MPD Brassicales Talus High-throughput Salzburger Spitzl 4 40.0154 54.3628 7.4260 483 -1.9321 0.0483 1 MPD Brassicales Talus High-throughput Snowyside Peak 4 58.6626 54.1972 7.6010 6750 0.5875 0.6749 0 MPD Brassicales Talus High-throughput Thompson Peak 2 55.8390 54.2070 19.1813 3165.5 0.0851 0.3165 0 MPD Brassicales Talus High-throughput Braxon Peak 2 44.3366 55.0534 26.7134 4609.5 -0.4012 0.4609 0 MPD Lamiales Talus High-throughput Castle Peak 2 81.0373 55.0739 26.4911 5968.5 0.9801 0.5968 0 MPD Lamiales Talus High-throughput D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Mount Cramer 3 57.1844 54.6853 14.7308 4693 0.1697 0.4693 0 MPD Lamiales Talus High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Thompson Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus High-throughput Braxon Peak 6 190.8280 160.0854 37.6152 7960.5 0.8173 0.7960 0 MPD Poales Talus High-throughput Castle Peak 7 167.9490 159.2159 34.2353 5445 0.2551 0.5444 0 MPD Poales Talus High-throughput D.O. Lee Peak 4 71.9904 160.4552 50.4361 758 -1.7540 0.0758 0 MPD Poales Talus High-throughput Horstmann Peak 6 204.4259 159.6996 38.0819 8854.5 1.1745 0.8854 0 MPD Poales Talus High-throughput Hyndman Peak 10 160.2096 159.2505 26.9085 4533 0.0356 0.4533 0 MPD Poales Talus High-throughput Mount Cramer 6 140.6756 159.7783 38.1470 3444 -0.5008 0.3444 0 MPD Poales Talus High-throughput Salzburger Spitzl 6 189.3423 158.8248 38.4958 7985 0.7927 0.7984 0 MPD Poales Talus High-throughput Snowyside Peak 4 72.2582 159.5984 50.1491 782 -1.7416 0.0782 0 MPD Poales Talus High-throughput Thompson Peak 5 198.7438 159.5914 43.0955 8333.5 0.9085 0.8333 0 MPD Poales Talus High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales Talus High-throughput Castle Peak 2 74.0450 129.5122 72.0602 3577 -0.7697 0.3577 0 MPD Ericales Talus High-throughput D.O. Lee Peak 4 134.7888 126.6824 37.9860 6005 0.2134 0.6004 0 MPD Ericales Talus High-throughput Horstmann Peak 2 193.2182 128.6207 72.3626 7524.5 0.8927 0.7524 0 MPD Ericales Talus High-throughput Hyndman Peak 3 57.5476 127.2464 48.5354 1383.5 -1.4360 0.1383 0 MPD Ericales Talus High-throughput Mount Cramer 3 77.7694 127.9323 48.4053 2752.5 -1.0363 0.2752 0 MPD Ericales Talus High-throughput

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Salzburger Spitzl 3 158.3859 127.9917 48.0274 8212 0.6328 0.8211 0 MPD Ericales Talus High-throughput Snowyside Peak 3 148.4702 128.5693 47.9497 5554.5 0.4150 0.5554 0 MPD Ericales Talus High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MPD Ericales Talus High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Tracheophyta Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Tracheophyta Meadow High-throughput D.O. Lee Peak 26 82.5788 104.7338 20.9709 1577 -1.0565 0.1577 0 MNTD Tracheophyta Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Tracheophyta Meadow High-throughput Hyndman Peak 26 74.5202 104.2214 20.9763 805 -1.4159 0.0805 0 MNTD Tracheophyta Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Tracheophyta Meadow High-throughput Salzburger Spitzl 23 96.7472 111.3461 23.4547 2843 -0.6224 0.2843 0 MNTD Tracheophyta Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Tracheophyta Meadow High-throughput Thompson Peak 26 111.2600 104.3509 21.1632 6242 0.3265 0.6241 0 MNTD Tracheophyta Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Asterales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Asterales Meadow High-throughput D.O. Lee Peak 9 31.0613 27.5543 9.1683 6583.5 0.3825 0.6583 0 MNTD Asterales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Asterales Meadow High-throughput Hyndman Peak 6 40.8316 36.0527 13.3454 6494.5 0.3581 0.6494 0 MNTD Asterales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Asterales Meadow High-throughput Salzburger Spitzl 4 52.9614 46.9402 18.7772 6958.5 0.3207 0.6958 0 MNTD Asterales Meadow High-throughput

55 Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Asterales Meadow High-throughput Thompson Peak 4 49.0501 47.0165 18.8083 5819 0.1081 0.5818 0 MNTD Asterales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput D.O. Lee Peak 5 38.8118 94.6062 27.2811 270 -2.0452 0.0270 1 MNTD Caryophyllales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput Hyndman Peak 5 91.2168 93.9689 27.4604 4375 -0.1002 0.4375 0 MNTD Caryophyllales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Caryophyllales Meadow High-throughput Thompson Peak 3 96.4426 134.4782 43.2026 2210.5 -0.8804 0.2210 0 MNTD Caryophyllales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Salzburger Spitzl 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput D.O. Lee Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput Hyndman Peak 4 56.1621 39.3637 15.9718 8494 1.0518 0.8493 0 MNTD Lamiales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput Salzburger Spitzl 5 26.5231 34.4016 12.8641 2900 -0.6124 0.2900 0 MNTD Lamiales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow High-throughput Thompson Peak 2 77.3210 58.8679 26.9321 5518.5 0.6852 0.5518 0 MNTD Lamiales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Poales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Poales Meadow High-throughput D.O. Lee Peak 3 147.4133 112.1692 58.8237 8459.5 0.5991 0.8459 0 MNTD Poales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Poales Meadow High-throughput

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summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Hyndman Peak 3 40.3918 113.7483 58.8772 1101 -1.2459 0.1101 0 MNTD Poales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Poales Meadow High-throughput Salzburger Spitzl 3 60.9261 112.9218 58.8177 1721.5 -0.8840 0.1721 0 MNTD Poales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Poales Meadow High-throughput Thompson Peak 3 97.2345 112.7223 58.6838 3833 -0.2639 0.3833 0 MNTD Poales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MNTD Ericales Meadow High-throughput D.O. Lee Peak 2 193.2182 106.6505 70.8897 8401 1.2212 0.8400 0 MNTD Ericales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MNTD Ericales Meadow High-throughput Hyndman Peak 2 38.1663 106.0084 71.1441 2682.5 -0.9536 0.2682 0 MNTD Ericales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MNTD Ericales Meadow High-throughput Salzburger Spitzl 2 38.1663 105.5274 70.4901 2017.5 -0.9556 0.2017 0 MNTD Ericales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Ericales Meadow High-throughput Thompson Peak 4 91.7804 59.7768 25.2944 9816 1.2652 0.9815 1 MNTD Ericales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Tracheophyta Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Tracheophyta Meadow High-throughput D.O. Lee Peak 26 242.1478 274.1550 29.3953 1440 -1.0889 0.1440 0 MPD Tracheophyta Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Tracheophyta Meadow High-throughput Hyndman Peak 26 246.1650 273.8644 29.2126 1818 -0.9482 0.1818 0 MPD Tracheophyta Meadow High-throughput

56 Mount Cramer 0 NA NA NA NA NA NA NA MPD Tracheophyta Meadow High-throughput Salzburger Spitzl 23 249.8160 273.7468 31.9751 2560 -0.7484 0.2560 0 MPD Tracheophyta Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Tracheophyta Meadow High-throughput Thompson Peak 26 353.4025 274.4482 29.3884 9955 2.6866 0.9954 1 MPD Tracheophyta Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Asterales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Asterales Meadow High-throughput D.O. Lee Peak 9 63.8307 68.0760 6.2467 2342 -0.6796 0.2342 0 MPD Asterales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Asterales Meadow High-throughput Hyndman Peak 6 74.8507 68.0349 9.1830 7601.5 0.7422 0.7601 0 MPD Asterales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Asterales Meadow High-throughput Salzburger Spitzl 4 75.3262 68.0754 13.0204 6911.5 0.5569 0.6911 0 MPD Asterales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Asterales Meadow High-throughput Thompson Peak 4 75.9107 67.9545 13.1868 7339.5 0.6033 0.7339 0 MPD Asterales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput D.O. Lee Peak 5 144.3537 166.3343 14.8131 1046.5 -1.4839 0.1046 0 MPD Caryophyllales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput Hyndman Peak 5 175.3571 166.0833 14.8094 7862.5 0.6262 0.7862 0 MPD Caryophyllales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Caryophyllales Meadow High-throughput Thompson Peak 3 134.2494 165.9335 32.9913 1280 -0.9604 0.1280 0 MPD Caryophyllales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Hyndman Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Salzburger Spitzl 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Thompson Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

D.O. Lee Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput Hyndman Peak 4 68.5169 59.5591 12.7835 7559.5 0.7007 0.7559 0 MPD Lamiales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput Salzburger Spitzl 5 40.4785 59.4292 10.1699 507 -1.8634 0.0507 0 MPD Lamiales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow High-throughput Thompson Peak 2 77.3210 59.4968 26.4758 5482 0.6732 0.5481 0 MPD Lamiales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Poales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Poales Meadow High-throughput D.O. Lee Peak 3 206.8798 155.5442 65.3188 8440.5 0.7859 0.8440 0 MPD Poales Meadow High-throughput Horstmann Peak 0 NA NA NA NA NA NA NA MPD Poales Meadow High-throughput Hyndman Peak 3 43.9038 154.9360 65.6702 702.5 -1.6908 0.0702 0 MPD Poales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Poales Meadow High-throughput Salzburger Spitzl 3 74.4365 156.7167 65.9580 1751 -1.2475 0.1751 0 MPD Poales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Poales Meadow High-throughput Thompson Peak 3 181.7904 155.3834 65.1005 5914 0.4056 0.5913 0 MPD Poales Meadow High-throughput Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales Meadow High-throughput Castle Peak 0 NA NA NA NA NA NA NA MPD Ericales Meadow High-throughput D.O. Lee Peak 2 193.2182 106.8044 71.1717 8390 1.2142 0.8389 0 MPD Ericales Meadow High-throughput

57 Horstmann Peak 0 NA NA NA NA NA NA NA MPD Ericales Meadow High-throughput Hyndman Peak 2 38.1663 106.9919 71.0685 2657 -0.9684 0.2657 0 MPD Ericales Meadow High-throughput Mount Cramer 0 NA NA NA NA NA NA NA MPD Ericales Meadow High-throughput Salzburger Spitzl 2 38.1663 106.0182 70.7937 1983.5 -0.9584 0.1983 0 MPD Ericales Meadow High-throughput Snowyside Peak 0 NA NA NA NA NA NA NA MPD Ericales Meadow High-throughput Thompson Peak 4 135.4938 105.4425 36.1909 8496.5 0.8304 0.8496 0 MPD Ericales Meadow High-throughput Braxon Peak 22 105.9556 139.6394 32.7251 1612 -1.0293 0.1612 0 MNTD Tracheophyta All Alpine GenBank Castle Peak 39 117.3953 103.1111 18.9609 7620 0.7533 0.7619 0 MNTD Tracheophyta All Alpine GenBank D.O. Lee Peak 41 66.3253 100.0002 17.9669 359 -1.8743 0.0359 1 MNTD Tracheophyta All Alpine GenBank Horstmann Peak 26 151.4916 128.7063 28.3844 7802 0.8027 0.7801 0 MNTD Tracheophyta All Alpine GenBank Hyndman Peak 60 65.0749 78.6230 11.3916 1255 -1.1893 0.1255 0 MNTD Tracheophyta All Alpine GenBank Mount Cramer 32 116.8802 115.3702 23.4013 5245 0.0645 0.5244 0 MNTD Tracheophyta All Alpine GenBank Salzburger Spitzl 53 90.5365 85.3918 13.1339 6384 0.3917 0.6383 0 MNTD Tracheophyta All Alpine GenBank Snowyside Peak 25 126.2095 130.8458 29.5229 4504 -0.1570 0.4504 0 MNTD Tracheophyta All Alpine GenBank Thompson Peak 41 71.7787 100.2818 18.0033 669 -1.5832 0.0669 0 MNTD Tracheophyta All Alpine GenBank Braxon Peak 3 66.9798 51.8420 20.7839 6094.5 0.7283 0.6094 0 MNTD Asterales All Alpine GenBank Castle Peak 9 26.4265 30.2545 8.3730 3350 -0.4572 0.3350 0 MNTD Asterales All Alpine GenBank D.O. Lee Peak 4 46.9869 45.6869 17.3145 5745 0.0751 0.5744 0 MNTD Asterales All Alpine GenBank Horstmann Peak 4 68.1155 45.5950 17.3296 7913.5 1.2995 0.7913 0 MNTD Asterales All Alpine GenBank Hyndman Peak 8 34.5044 32.3410 9.4841 5783 0.2281 0.5782 0 MNTD Asterales All Alpine GenBank Mount Cramer 8 29.8836 32.1166 9.4718 4246.5 -0.2358 0.4246 0 MNTD Asterales All Alpine GenBank Salzburger Spitzl 7 43.1620 34.5685 10.9087 7825.5 0.7878 0.7825 0 MNTD Asterales All Alpine GenBank Snowyside Peak 5 23.3316 40.9530 14.5210 1105.5 -1.2135 0.1105 0 MNTD Asterales All Alpine GenBank Thompson Peak 3 66.9798 51.9358 20.8752 6062 0.7207 0.6061 0 MNTD Asterales All Alpine GenBank Braxon Peak 4 112.1998 98.8349 15.1928 6670.5 0.8797 0.6670 0 MNTD Caryophyllales All Alpine GenBank Castle Peak 4 112.1998 98.6313 15.4806 6643.5 0.8765 0.6643 0 MNTD Caryophyllales All Alpine GenBank D.O. Lee Peak 5 95.1000 88.8693 11.1609 6663 0.5583 0.6662 0 MNTD Caryophyllales All Alpine GenBank Horstmann Peak 2 102.3991 138.0185 44.3492 2474.5 -0.8032 0.2474 0 MNTD Caryophyllales All Alpine GenBank Hyndman Peak 5 95.1000 88.6117 11.0975 6771.5 0.5847 0.6771 0 MNTD Caryophyllales All Alpine GenBank Mount Cramer 5 92.9561 88.7950 11.1248 5414 0.3740 0.5413 0 MNTD Caryophyllales All Alpine GenBank Salzburger Spitzl 7 69.3353 76.2639 7.2283 2378 -0.9585 0.2378 0 MNTD Caryophyllales All Alpine GenBank Snowyside Peak 3 127.9067 113.2474 22.6644 5880 0.6468 0.5879 0 MNTD Caryophyllales All Alpine GenBank Thompson Peak 2 178.9219 138.4198 44.5219 7885 0.9097 0.7884 0 MNTD Caryophyllales All Alpine GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Braxon Peak 5 59.8484 81.0658 31.6795 2280 -0.6698 0.2280 0 MNTD Poales All Alpine GenBank Castle Peak 6 69.1370 61.7580 22.6132 5865 0.3263 0.5864 0 MNTD Poales All Alpine GenBank D.O. Lee Peak 3 100.1612 143.6239 71.0697 2911.5 -0.6116 0.2911 0 MNTD Poales All Alpine GenBank Horstmann Peak 5 127.4815 80.8399 31.7062 9764.5 1.4711 0.9764 1 MNTD Poales All Alpine GenBank Hyndman Peak 8 57.1238 38.7388 13.5079 8982 1.3610 0.8981 0 MNTD Poales All Alpine GenBank Mount Cramer 3 119.2872 144.1508 71.6223 5726.5 -0.3471 0.5726 0 MNTD Poales All Alpine GenBank Salzburger Spitzl 5 34.7425 80.9186 31.5458 1480.5 -1.4638 0.1480 0 MNTD Poales All Alpine GenBank Snowyside Peak 4 24.8969 107.4890 47.2548 557 -1.7478 0.0557 0 MNTD Poales All Alpine GenBank Thompson Peak 4 145.4002 107.4332 47.1077 7246 0.8060 0.7245 0 MNTD Poales All Alpine GenBank Braxon Peak 2 14.0308 52.6740 34.5003 3018 -1.1201 0.3018 0 MNTD Lamiales All Alpine GenBank Castle Peak 2 81.0373 53.6355 34.3262 6954 0.7983 0.6953 0 MNTD Lamiales All Alpine GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Mount Cramer 3 29.6638 31.5179 7.1348 2530.5 -0.2599 0.2530 0 MNTD Lamiales All Alpine GenBank Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Thompson Peak 0 NA NA NA NA NA NA NA MNTD Lamiales All Alpine GenBank Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales All Alpine GenBank

58 Castle Peak 5 3.0183 14.5736 5.5610 372.5 -2.0779 0.0372 1 MNTD Brassicales All Alpine GenBank D.O. Lee Peak 4 21.3587 17.1772 7.4780 7424.5 0.5592 0.7424 0 MNTD Brassicales All Alpine GenBank Horstmann Peak 2 41.4371 29.1729 16.5815 9706 0.7396 0.9705 1 MNTD Brassicales All Alpine GenBank Hyndman Peak 5 18.2739 14.4203 5.6068 7372.5 0.6873 0.7372 0 MNTD Brassicales All Alpine GenBank Mount Cramer 2 29.7222 29.1827 16.5512 3368.5 0.0326 0.3368 0 MNTD Brassicales All Alpine GenBank Salzburger Spitzl 2 38.0885 29.5065 16.4008 3854 0.5233 0.3854 0 MNTD Brassicales All Alpine GenBank Snowyside Peak 2 38.0885 29.4352 16.4626 3849 0.5256 0.3849 0 MNTD Brassicales All Alpine GenBank Thompson Peak 2 38.0885 29.1635 16.5567 3935.5 0.5391 0.3935 0 MNTD Brassicales All Alpine GenBank Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales All Alpine GenBank Castle Peak 2 33.8479 128.4767 60.9558 1089.5 -1.5524 0.1089 0 MNTD Ericales All Alpine GenBank D.O. Lee Peak 3 83.5996 98.7081 42.0250 4030.5 -0.3595 0.4030 0 MNTD Ericales All Alpine GenBank Horstmann Peak 2 170.4198 127.4776 61.1829 8964.5 0.7019 0.8964 0 MNTD Ericales All Alpine GenBank Hyndman Peak 4 53.1470 78.0320 28.7526 1304 -0.8655 0.1304 0 MNTD Ericales All Alpine GenBank Mount Cramer 3 40.7003 99.2497 42.1819 812.5 -1.3880 0.0812 0 MNTD Ericales All Alpine GenBank Salzburger Spitzl 3 77.8773 98.4911 42.4249 2729 -0.4859 0.2729 0 MNTD Ericales All Alpine GenBank Snowyside Peak 3 85.0942 98.7710 42.1313 4727 -0.3246 0.4727 0 MNTD Ericales All Alpine GenBank Thompson Peak 1 NA NA NA NA NA NA NA MNTD Ericales All Alpine GenBank Braxon Peak 22 358.8205 323.8757 52.7807 7458 0.6621 0.7457 0 MPD Tracheophyta All Alpine GenBank Castle Peak 39 345.0887 323.4932 36.0846 7269 0.5985 0.7268 0 MPD Tracheophyta All Alpine GenBank D.O. Lee Peak 41 253.8235 324.2441 35.0026 206 -2.0119 0.0206 1 MPD Tracheophyta All Alpine GenBank Horstmann Peak 26 454.3907 323.8983 46.8996 9966 2.7824 0.9965 1 MPD Tracheophyta All Alpine GenBank Hyndman Peak 60 280.6326 324.4899 24.9423 403 -1.7584 0.0403 1 MPD Tracheophyta All Alpine GenBank Mount Cramer 32 312.5965 323.9471 41.4422 4019 -0.2739 0.4019 0 MPD Tracheophyta All Alpine GenBank Salzburger Spitzl 53 304.0246 323.7445 27.9621 2462 -0.7052 0.2462 0 MPD Tracheophyta All Alpine GenBank Snowyside Peak 25 422.3551 324.1044 48.2459 9719 2.0365 0.9718 1 MPD Tracheophyta All Alpine GenBank Thompson Peak 41 327.6563 323.4917 34.6511 5474 0.1202 0.5473 0 MPD Tracheophyta All Alpine GenBank Braxon Peak 3 70.0696 62.4998 16.0939 6055 0.4704 0.6054 0 MPD Asterales All Alpine GenBank Castle Peak 9 65.2161 62.4089 6.3902 6385.5 0.4393 0.6385 0 MPD Asterales All Alpine GenBank D.O. Lee Peak 4 66.3086 62.3573 12.7766 5482.5 0.3093 0.5482 0 MPD Asterales All Alpine GenBank Horstmann Peak 4 71.0689 62.5801 12.6862 7619.5 0.6691 0.7619 0 MPD Asterales All Alpine GenBank Hyndman Peak 8 51.9463 62.4705 7.0570 867 -1.4913 0.0867 0 MPD Asterales All Alpine GenBank Mount Cramer 8 59.1294 62.5206 7.1291 2790 -0.4757 0.2790 0 MPD Asterales All Alpine GenBank Salzburger Spitzl 7 69.9370 62.4740 7.9466 8314.5 0.9392 0.8314 0 MPD Asterales All Alpine GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Snowyside Peak 5 56.9947 62.5603 10.4436 2471 -0.5329 0.2471 0 MPD Asterales All Alpine GenBank Thompson Peak 3 70.0696 62.5064 16.2762 6000.5 0.4647 0.6000 0 MPD Asterales All Alpine GenBank Braxon Peak 4 156.6812 137.6974 16.7944 9054 1.1304 0.9053 0 MPD Caryophyllales All Alpine GenBank Castle Peak 4 156.6812 137.6450 16.9106 9022 1.1257 0.9021 0 MPD Caryophyllales All Alpine GenBank D.O. Lee Peak 5 149.4282 138.1648 11.7713 8507 0.9569 0.8506 0 MPD Caryophyllales All Alpine GenBank Horstmann Peak 2 102.3991 138.0504 44.6306 2487.5 -0.7988 0.2487 0 MPD Caryophyllales All Alpine GenBank Hyndman Peak 5 149.4282 137.8183 12.2140 8586 0.9505 0.8585 0 MPD Caryophyllales All Alpine GenBank Mount Cramer 5 148.8922 137.9995 11.8821 8170 0.9167 0.8169 0 MPD Caryophyllales All Alpine GenBank Salzburger Spitzl 7 144.3494 137.9013 6.2255 9580.5 1.0357 0.9580 1 MPD Caryophyllales All Alpine GenBank Snowyside Peak 3 153.4143 138.3571 24.8010 5750.5 0.6071 0.5750 0 MPD Caryophyllales All Alpine GenBank Thompson Peak 2 178.9219 137.9882 44.6291 7915.5 0.9172 0.7915 0 MPD Caryophyllales All Alpine GenBank Braxon Peak 5 223.8651 199.4415 31.2788 7567 0.7808 0.7566 0 MPD Poales All Alpine GenBank Castle Peak 6 119.9533 199.7366 24.2338 58.5 -3.2922 0.0058 1 MPD Poales All Alpine GenBank D.O. Lee Peak 3 166.3981 198.7103 61.2781 2915 -0.5273 0.2915 0 MPD Poales All Alpine GenBank Horstmann Peak 5 198.5702 199.7730 31.0558 4531 -0.0387 0.4531 0 MPD Poales All Alpine GenBank Hyndman Peak 8 182.7706 199.4752 15.4072 2127 -1.0842 0.2127 0 MPD Poales All Alpine GenBank Mount Cramer 3 204.6500 199.3407 60.7564 5737.5 0.0874 0.5737 0 MPD Poales All Alpine GenBank Salzburger Spitzl 5 155.3032 199.2355 31.4185 1769 -1.3983 0.1769 0 MPD Poales All Alpine GenBank Snowyside Peak 4 36.3262 199.2428 42.3986 69.5 -3.8425 0.0069 1 MPD Poales All Alpine GenBank

59 Thompson Peak 4 227.4642 198.5514 41.9967 6893.5 0.6885 0.6893 0 MPD Poales All Alpine GenBank Braxon Peak 2 14.0308 53.2372 34.4495 2979.5 -1.1381 0.2979 0 MPD Lamiales All Alpine GenBank Castle Peak 2 81.0373 52.3428 34.6035 7045.5 0.8292 0.7045 0 MPD Lamiales All Alpine GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Mount Cramer 3 55.3506 53.1614 13.0952 2457 0.1672 0.2457 0 MPD Lamiales All Alpine GenBank Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Thompson Peak 0 NA NA NA NA NA NA NA MPD Lamiales All Alpine GenBank Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales All Alpine GenBank Castle Peak 5 26.5070 29.3651 5.3487 2014.5 -0.5343 0.2014 0 MPD Brassicales All Alpine GenBank D.O. Lee Peak 4 34.7443 29.3394 7.1595 7434 0.7549 0.7433 0 MPD Brassicales All Alpine GenBank Horstmann Peak 2 41.4371 29.4200 16.4430 9727 0.7308 0.9726 1 MPD Brassicales All Alpine GenBank Hyndman Peak 5 30.3209 29.3259 5.4163 5830 0.1837 0.5829 0 MPD Brassicales All Alpine GenBank Mount Cramer 2 29.7222 29.1457 16.5524 3379 0.0348 0.3379 0 MPD Brassicales All Alpine GenBank Salzburger Spitzl 2 38.0885 29.4766 16.4655 3825 0.5230 0.3825 0 MPD Brassicales All Alpine GenBank Snowyside Peak 2 38.0885 29.1240 16.5843 3940.5 0.5405 0.3940 0 MPD Brassicales All Alpine GenBank Thompson Peak 2 38.0885 29.2484 16.5412 3903.5 0.5344 0.3903 0 MPD Brassicales All Alpine GenBank Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales All Alpine GenBank Castle Peak 2 33.8479 128.7133 60.7273 1094.5 -1.5622 0.1094 0 MPD Ericales All Alpine GenBank D.O. Lee Peak 3 124.7678 129.4823 33.3564 2937.5 -0.1413 0.2937 0 MPD Ericales All Alpine GenBank Horstmann Peak 2 170.4198 129.8027 60.2882 8934 0.6737 0.8933 0 MPD Ericales All Alpine GenBank Hyndman Peak 4 95.3177 128.6357 22.7346 240 -1.4655 0.0240 1 MPD Ericales All Alpine GenBank Mount Cramer 3 63.9027 128.9799 33.9171 816 -1.9187 0.0816 0 MPD Ericales All Alpine GenBank Salzburger Spitzl 3 121.9067 128.9469 33.3643 2703 -0.2110 0.2703 0 MPD Ericales All Alpine GenBank Snowyside Peak 3 127.7570 129.1367 33.3765 4724.5 -0.0413 0.4724 0 MPD Ericales All Alpine GenBank Thompson Peak 1 NA NA NA NA NA NA NA MPD Ericales All Alpine GenBank Braxon Peak 22 105.9556 141.9050 33.0366 1496 -1.0882 0.1496 0 MNTD Tracheophyta Talus GenBank Castle Peak 39 117.3953 101.3309 18.1762 8029 0.8838 0.8028 0 MNTD Tracheophyta Talus GenBank D.O. Lee Peak 25 82.2346 132.6868 29.1566 431 -1.7304 0.0431 1 MNTD Tracheophyta Talus GenBank Horstmann Peak 26 151.4916 129.9605 28.3572 7644 0.7593 0.7643 0 MNTD Tracheophyta Talus GenBank Hyndman Peak 37 55.3230 105.1605 19.2124 35 -2.5940 0.0035 1 MNTD Tracheophyta Talus GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Mount Cramer 32 116.8802 115.3762 22.8141 5161 0.0659 0.5160 0 MNTD Tracheophyta Talus GenBank Salzburger Spitzl 36 108.6919 107.0630 19.9601 5185 0.0816 0.5184 0 MNTD Tracheophyta Talus GenBank Snowyside Peak 25 126.2095 133.4273 29.2492 4076 -0.2468 0.4076 0 MNTD Tracheophyta Talus GenBank Thompson Peak 14 133.5779 173.8889 47.6187 2193 -0.8465 0.2193 0 MNTD Tracheophyta Talus GenBank Braxon Peak 3 66.9798 52.0369 20.9533 5992.5 0.7132 0.5992 0 MNTD Asterales Talus GenBank Castle Peak 9 26.4265 30.3020 8.4198 3334 -0.4603 0.3334 0 MNTD Asterales Talus GenBank D.O. Lee Peak 4 46.9869 45.3592 17.3239 5828.5 0.0940 0.5828 0 MNTD Asterales Talus GenBank Horstmann Peak 4 68.1155 45.3497 17.1373 7998 1.3284 0.7997 0 MNTD Asterales Talus GenBank Hyndman Peak 8 34.5044 32.2885 9.4828 5784 0.2337 0.5783 0 MNTD Asterales Talus GenBank Mount Cramer 8 29.8836 32.3816 9.5117 4125.5 -0.2626 0.4125 0 MNTD Asterales Talus GenBank Salzburger Spitzl 7 43.1620 34.5532 10.6588 7893.5 0.8077 0.7893 0 MNTD Asterales Talus GenBank Snowyside Peak 5 23.3316 40.8553 14.4032 1068 -1.2167 0.1068 0 MNTD Asterales Talus GenBank Thompson Peak 3 66.9798 52.1347 20.7553 5976.5 0.7152 0.5976 0 MNTD Asterales Talus GenBank Braxon Peak 4 112.1998 98.7118 15.0545 6729.5 0.8959 0.6729 0 MNTD Caryophyllales Talus GenBank Castle Peak 4 112.1998 98.8426 15.2374 6651 0.8766 0.6650 0 MNTD Caryophyllales Talus GenBank D.O. Lee Peak 5 95.1000 88.8141 11.0692 6713 0.5679 0.6712 0 MNTD Caryophyllales Talus GenBank Horstmann Peak 2 102.3991 137.7845 44.5939 2514.5 -0.7935 0.2514 0 MNTD Caryophyllales Talus GenBank Hyndman Peak 5 95.1000 88.9647 11.1318 6607 0.5511 0.6606 0 MNTD Caryophyllales Talus GenBank Mount Cramer 5 92.9561 88.8737 11.1595 5393 0.3658 0.5392 0 MNTD Caryophyllales Talus GenBank

60 Salzburger Spitzl 7 69.3353 76.3243 7.1873 2331.5 -0.9724 0.2331 0 MNTD Caryophyllales Talus GenBank Snowyside Peak 3 127.9067 113.4245 22.5934 5909.5 0.6410 0.5909 0 MNTD Caryophyllales Talus GenBank Thompson Peak 2 178.9219 138.9257 44.4980 7850.5 0.8988 0.7850 0 MNTD Caryophyllales Talus GenBank Braxon Peak 5 59.8484 80.7955 31.6893 2270.5 -0.6610 0.2270 0 MNTD Poales Talus GenBank Castle Peak 6 69.1370 61.7987 22.7945 5804 0.3219 0.5803 0 MNTD Poales Talus GenBank D.O. Lee Peak 3 100.1612 143.4008 70.7544 2923.5 -0.6111 0.2923 0 MNTD Poales Talus GenBank Horstmann Peak 5 127.4815 80.8462 31.3635 9777 1.4869 0.9776 1 MNTD Poales Talus GenBank Hyndman Peak 8 57.1238 38.9925 13.5452 8955.5 1.3386 0.8955 0 MNTD Poales Talus GenBank Mount Cramer 3 119.2872 144.1213 70.9194 5693.5 -0.3502 0.5693 0 MNTD Poales Talus GenBank Salzburger Spitzl 5 34.7425 80.4300 31.6454 1507 -1.4437 0.1507 0 MNTD Poales Talus GenBank Snowyside Peak 4 24.8969 106.7275 47.1160 553 -1.7368 0.0553 0 MNTD Poales Talus GenBank Thompson Peak 4 145.4002 107.6819 47.4460 7159 0.7950 0.7158 0 MNTD Poales Talus GenBank Braxon Peak 2 14.0308 53.0122 34.4885 3023 -1.1303 0.3023 0 MNTD Lamiales Talus GenBank Castle Peak 2 81.0373 52.5780 34.5085 7032 0.8247 0.7031 0 MNTD Lamiales Talus GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Horstmann Peak 1 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Mount Cramer 3 29.6638 31.5979 7.0704 2495.5 -0.2736 0.2495 0 MNTD Lamiales Talus GenBank Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Snowyside Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Thompson Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Talus GenBank Braxon Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Talus GenBank Castle Peak 5 3.0183 14.5349 5.5269 386.5 -2.0837 0.0386 1 MNTD Brassicales Talus GenBank D.O. Lee Peak 4 21.3587 17.3143 7.4690 7412.5 0.5415 0.7412 0 MNTD Brassicales Talus GenBank Horstmann Peak 2 41.4371 29.5913 16.3793 9724.5 0.7232 0.9724 1 MNTD Brassicales Talus GenBank Hyndman Peak 5 18.2739 14.5422 5.4923 7423.5 0.6794 0.7423 0 MNTD Brassicales Talus GenBank Mount Cramer 2 29.7222 29.4196 16.4636 3301.5 0.0184 0.3301 0 MNTD Brassicales Talus GenBank Salzburger Spitzl 2 38.0885 29.3275 16.4727 3892 0.5319 0.3892 0 MNTD Brassicales Talus GenBank Snowyside Peak 2 38.0885 29.0635 16.5721 3963 0.5446 0.3963 0 MNTD Brassicales Talus GenBank Thompson Peak 2 38.0885 29.4211 16.4618 3863.5 0.5265 0.3863 0 MNTD Brassicales Talus GenBank Braxon Peak 0 NA NA NA NA NA NA NA MNTD Ericales Talus GenBank Castle Peak 2 33.8479 129.6148 60.3157 1056 -1.5878 0.1056 0 MNTD Ericales Talus GenBank D.O. Lee Peak 3 83.5996 98.7408 42.1718 4057.5 -0.3590 0.4057 0 MNTD Ericales Talus GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Horstmann Peak 2 170.4198 129.1057 60.5218 8925.5 0.6826 0.8925 0 MNTD Ericales Talus GenBank Hyndman Peak 4 53.1470 78.0414 28.8361 1341 -0.8633 0.1341 0 MNTD Ericales Talus GenBank Mount Cramer 3 40.7003 98.4364 42.0650 816 -1.3725 0.0816 0 MNTD Ericales Talus GenBank Salzburger Spitzl 3 77.8773 98.4998 42.0981 2733.5 -0.4899 0.2733 0 MNTD Ericales Talus GenBank Snowyside Peak 3 85.0942 98.7089 41.9992 4764.5 -0.3242 0.4764 0 MNTD Ericales Talus GenBank Thompson Peak 1 NA NA NA NA NA NA NA MNTD Ericales Talus GenBank Braxon Peak 22 358.8205 337.9150 54.8839 6488 0.3809 0.6487 0 MPD Tracheophyta Talus GenBank Castle Peak 39 345.0887 338.4546 36.6820 5718 0.1809 0.5717 0 MPD Tracheophyta Talus GenBank D.O. Lee Peak 25 254.6728 337.9822 51.1947 517 -1.6273 0.0517 0 MPD Tracheophyta Talus GenBank Horstmann Peak 26 454.3907 338.1560 49.4388 9902 2.3511 0.9901 1 MPD Tracheophyta Talus GenBank Hyndman Peak 37 269.0644 337.8325 37.6816 314 -1.8250 0.0314 1 MPD Tracheophyta Talus GenBank Mount Cramer 32 312.5965 338.4835 42.9197 2767 -0.6031 0.2767 0 MPD Tracheophyta Talus GenBank Salzburger Spitzl 36 329.8553 338.3085 39.0357 4154 -0.2165 0.4154 0 MPD Tracheophyta Talus GenBank Snowyside Peak 25 422.3551 336.4454 50.8377 9487 1.6899 0.9486 0 MPD Tracheophyta Talus GenBank Thompson Peak 14 289.4024 338.6139 72.3487 2819 -0.6802 0.2819 0 MPD Tracheophyta Talus GenBank Braxon Peak 3 70.0696 62.7260 16.1784 5955.5 0.4539 0.5955 0 MPD Asterales Talus GenBank Castle Peak 9 65.2161 62.4272 6.3339 6384 0.4403 0.6383 0 MPD Asterales Talus GenBank D.O. Lee Peak 4 66.3086 62.3988 12.6415 5560 0.3093 0.5559 0 MPD Asterales Talus GenBank Horstmann Peak 4 71.0689 62.4704 12.8202 7674 0.6707 0.7673 0 MPD Asterales Talus GenBank

61 Hyndman Peak 8 51.9463 62.4980 7.1543 949.5 -1.4749 0.0949 0 MPD Asterales Talus GenBank Mount Cramer 8 59.1294 62.5041 7.1116 2811.5 -0.4745 0.2811 0 MPD Asterales Talus GenBank Salzburger Spitzl 7 69.9370 62.4915 8.0260 8283 0.9277 0.8282 0 MPD Asterales Talus GenBank Snowyside Peak 5 56.9947 62.3785 10.6595 2580.5 -0.5051 0.2580 0 MPD Asterales Talus GenBank Thompson Peak 3 70.0696 62.5011 16.2831 6031.5 0.4648 0.6031 0 MPD Asterales Talus GenBank Braxon Peak 4 156.6812 137.8220 16.8135 9050.5 1.1217 0.9050 0 MPD Caryophyllales Talus GenBank Castle Peak 4 156.6812 137.8319 16.6731 9085 1.1305 0.9084 0 MPD Caryophyllales Talus GenBank D.O. Lee Peak 5 149.4282 138.0505 11.8234 8548.5 0.9623 0.8548 0 MPD Caryophyllales Talus GenBank Horstmann Peak 2 102.3991 138.5480 44.4318 2468 -0.8136 0.2468 0 MPD Caryophyllales Talus GenBank Hyndman Peak 5 149.4282 137.8882 11.9965 8552.5 0.9619 0.8552 0 MPD Caryophyllales Talus GenBank Mount Cramer 5 148.8922 138.1067 11.6395 8149 0.9266 0.8148 0 MPD Caryophyllales Talus GenBank Salzburger Spitzl 7 144.3494 137.9705 6.1237 9616 1.0417 0.9615 1 MPD Caryophyllales Talus GenBank Snowyside Peak 3 153.4143 138.0090 24.7662 5853.5 0.6220 0.5853 0 MPD Caryophyllales Talus GenBank Thompson Peak 2 178.9219 137.5801 44.6682 7943.5 0.9255 0.7943 0 MPD Caryophyllales Talus GenBank Braxon Peak 5 223.8651 198.9149 31.7688 7595 0.7854 0.7594 0 MPD Poales Talus GenBank Castle Peak 6 119.9533 199.4631 23.9962 46.5 -3.3134 0.0046 1 MPD Poales Talus GenBank D.O. Lee Peak 3 166.3981 200.5086 60.8128 2813.5 -0.5609 0.2813 0 MPD Poales Talus GenBank Horstmann Peak 5 198.5702 199.5537 31.2403 4549.5 -0.0315 0.4549 0 MPD Poales Talus GenBank Hyndman Peak 8 182.7706 199.4592 15.2035 2098 -1.0977 0.2098 0 MPD Poales Talus GenBank Mount Cramer 3 204.6500 199.8590 60.8228 5655.5 0.0788 0.5655 0 MPD Poales Talus GenBank Salzburger Spitzl 5 155.3032 199.5019 31.2956 1742 -1.4123 0.1742 0 MPD Poales Talus GenBank Snowyside Peak 4 36.3262 199.1850 41.8522 67 -3.8913 0.0067 1 MPD Poales Talus GenBank Thompson Peak 4 227.4642 199.1953 41.9190 6819 0.6744 0.6818 0 MPD Poales Talus GenBank Braxon Peak 2 14.0308 53.1260 34.4412 2992 -1.1351 0.2992 0 MPD Lamiales Talus GenBank Castle Peak 2 81.0373 53.1098 34.4406 6991.5 0.8109 0.6991 0 MPD Lamiales Talus GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Horstmann Peak 1 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Mount Cramer 3 55.3506 52.8965 13.4565 2502 0.1824 0.2502 0 MPD Lamiales Talus GenBank Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Snowyside Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Thompson Peak 0 NA NA NA NA NA NA NA MPD Lamiales Talus GenBank Braxon Peak 1 NA NA NA NA NA NA NA MPD Brassicales Talus GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Castle Peak 5 26.5070 29.3049 5.3258 2059.5 -0.5253 0.2059 0 MPD Brassicales Talus GenBank D.O. Lee Peak 4 34.7443 29.2549 7.3024 7479 0.7517 0.7478 0 MPD Brassicales Talus GenBank Horstmann Peak 2 41.4371 29.6457 16.3620 9703 0.7207 0.9702 1 MPD Brassicales Talus GenBank Hyndman Peak 5 30.3209 29.3106 5.4476 5828 0.1855 0.5827 0 MPD Brassicales Talus GenBank Mount Cramer 2 29.7222 29.0648 16.6125 3406.5 0.0396 0.3406 0 MPD Brassicales Talus GenBank Salzburger Spitzl 2 38.0885 29.4059 16.4375 3873.5 0.5282 0.3873 0 MPD Brassicales Talus GenBank Snowyside Peak 2 38.0885 29.1437 16.5560 3928 0.5403 0.3928 0 MPD Brassicales Talus GenBank Thompson Peak 2 38.0885 29.3186 16.5097 3877 0.5312 0.3877 0 MPD Brassicales Talus GenBank Braxon Peak 0 NA NA NA NA NA NA NA MPD Ericales Talus GenBank Castle Peak 2 33.8479 129.0384 60.7400 1118.5 -1.5672 0.1118 0 MPD Ericales Talus GenBank D.O. Lee Peak 3 124.7678 128.6990 34.2870 2920.5 -0.1147 0.2920 0 MPD Ericales Talus GenBank Horstmann Peak 2 170.4198 128.7549 60.9906 8912.5 0.6831 0.8912 0 MPD Ericales Talus GenBank Hyndman Peak 4 95.3177 128.9576 22.4402 216 -1.4991 0.0216 1 MPD Ericales Talus GenBank Mount Cramer 3 63.9027 129.2320 33.0795 773.5 -1.9749 0.0773 0 MPD Ericales Talus GenBank Salzburger Spitzl 3 121.9067 128.7803 33.6887 2732 -0.2040 0.2732 0 MPD Ericales Talus GenBank Snowyside Peak 3 127.7570 129.1244 33.4597 4730.5 -0.0409 0.4730 0 MPD Ericales Talus GenBank Thompson Peak 1 NA NA NA NA NA NA NA MPD Ericales Talus GenBank D.O. Lee Peak 21 88.3649 138.4899 31.6564 544 -1.5834 0.0544 0 MNTD Tracheophyta Meadow GenBank Hyndman Peak 23 114.0493 133.4990 29.2864 2615 -0.6641 0.2615 0 MNTD Tracheophyta Meadow GenBank

62 Salzburger Spitzl 19 99.6143 145.6828 34.4909 907 -1.3357 0.0907 0 MNTD Tracheophyta Meadow GenBank Thompson Peak 29 98.7237 119.5256 23.3272 1935 -0.8917 0.1935 0 MNTD Tracheophyta Meadow GenBank D.O. Lee Peak 5 34.4764 43.7851 12.5394 2594 -0.7424 0.2594 0 MNTD Asterales Meadow GenBank Hyndman Peak 5 52.8187 43.7217 12.6099 8415 0.7214 0.8414 0 MNTD Asterales Meadow GenBank Salzburger Spitzl 2 63.8899 64.7857 21.8850 3034.5 -0.0409 0.3034 0 MNTD Asterales Meadow GenBank Thompson Peak 4 44.7681 48.3525 15.3828 4422.5 -0.2330 0.4422 0 MNTD Asterales Meadow GenBank D.O. Lee Peak 4 18.5199 90.5733 22.0219 29.5 -3.2719 0.0029 1 MNTD Caryophyllales Meadow GenBank Hyndman Peak 4 114.1622 90.4858 22.3889 9129.5 1.0575 0.9129 0 MNTD Caryophyllales Meadow GenBank Salzburger Spitzl 2 178.9219 132.3810 49.9343 8304.5 0.9320 0.8304 0 MNTD Caryophyllales Meadow GenBank Thompson Peak 4 58.0503 90.3878 22.4530 1145 -1.4402 0.1145 0 MNTD Caryophyllales Meadow GenBank D.O. Lee Peak 3 100.1612 126.0306 68.1091 6009 -0.3798 0.6008 0 MNTD Poales Meadow GenBank Hyndman Peak 2 26.9105 180.6784 107.5169 1058 -1.4302 0.1058 0 MNTD Poales Meadow GenBank Salzburger Spitzl 3 29.2485 126.3225 69.4834 760 -1.3971 0.0760 0 MNTD Poales Meadow GenBank Thompson Peak 5 118.6813 73.5510 36.4499 7920 1.2381 0.7919 0 MNTD Poales Meadow GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MNTD Lamiales Meadow GenBank Hyndman Peak 4 50.4918 39.1478 14.0323 8060.5 0.8084 0.8060 0 MNTD Lamiales Meadow GenBank Salzburger Spitzl 5 23.9669 33.0257 10.0083 2376.5 -0.9051 0.2376 0 MNTD Lamiales Meadow GenBank Thompson Peak 2 57.3069 62.4206 26.6108 2865 -0.1922 0.2865 0 MNTD Lamiales Meadow GenBank D.O. Lee Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow GenBank Salzburger Spitzl 0 NA NA NA NA NA NA NA MNTD Brassicales Meadow GenBank Thompson Peak 1 NA NA NA NA NA NA NA MNTD Brassicales Meadow GenBank D.O. Lee Peak 2 170.4198 116.9675 57.9501 7517.5 0.9224 0.7517 0 MNTD Ericales Meadow GenBank Hyndman Peak 2 17.4979 116.7311 58.1033 839.5 -1.7079 0.0839 0 MNTD Ericales Meadow GenBank Salzburger Spitzl 1 NA NA NA NA NA NA NA MNTD Ericales Meadow GenBank Thompson Peak 4 73.1302 73.1302 0.0000 5001 NA 0.5000 0 MNTD Ericales Meadow GenBank D.O. Lee Peak 21 255.7319 317.3604 44.8842 893 -1.3731 0.0893 0 MPD Tracheophyta Meadow GenBank Hyndman Peak 23 296.9823 318.4823 41.5109 3127 -0.5179 0.3127 0 MPD Tracheophyta Meadow GenBank Salzburger Spitzl 19 256.5121 317.5431 48.2661 1188 -1.2645 0.1188 0 MPD Tracheophyta Meadow GenBank Thompson Peak 29 353.0725 317.9291 33.8907 8308 1.0370 0.8307 0 MPD Tracheophyta Meadow GenBank D.O. Lee Peak 5 53.0343 64.6516 8.2921 895 -1.4010 0.0895 0 MPD Asterales Meadow GenBank Hyndman Peak 5 69.7948 64.4145 8.3187 7224 0.6468 0.7223 0 MPD Asterales Meadow GenBank Salzburger Spitzl 2 63.8899 64.8671 21.9509 3057.5 -0.0445 0.3057 0 MPD Asterales Meadow GenBank

Continued on next page Table Appendix S7 – continued from previous page

summit N taxa obs rand mean rand sd obs rank obs z P-value metric sig clade species pool dataset

Thompson Peak 4 65.5690 64.3932 10.6647 4401.5 0.1103 0.4401 0 MPD Asterales Meadow GenBank D.O. Lee Peak 4 125.4546 132.0870 20.0073 2079.5 -0.3315 0.2079 0 MPD Caryophyllales Meadow GenBank Hyndman Peak 4 143.1050 131.8488 20.0269 7364 0.5621 0.7363 0 MPD Caryophyllales Meadow GenBank Salzburger Spitzl 2 178.9219 131.9251 49.9887 8322.5 0.9401 0.8322 0 MPD Caryophyllales Meadow GenBank Thompson Peak 4 100.6838 131.8929 19.8703 1549 -1.5706 0.1549 0 MPD Caryophyllales Meadow GenBank D.O. Lee Peak 3 166.3981 181.4256 63.4798 5948 -0.2367 0.5947 0 MPD Poales Meadow GenBank Hyndman Peak 2 26.9105 179.9327 108.0134 1102 -1.4167 0.1102 0 MPD Poales Meadow GenBank Salzburger Spitzl 3 31.5864 181.6856 63.6136 711 -2.3595 0.0711 0 MPD Poales Meadow GenBank Thompson Peak 5 193.7551 181.7494 31.4924 4628 0.3812 0.4628 0 MPD Poales Meadow GenBank D.O. Lee Peak 0 NA NA NA NA NA NA NA MPD Lamiales Meadow GenBank Hyndman Peak 4 70.8555 62.1645 10.1742 7996.5 0.8542 0.7996 0 MPD Lamiales Meadow GenBank Salzburger Spitzl 5 43.6553 62.3862 6.7779 251.5 -2.7636 0.0251 1 MPD Lamiales Meadow GenBank Thompson Peak 2 57.3069 62.0899 27.1918 2851.5 -0.1759 0.2851 0 MPD Lamiales Meadow GenBank D.O. Lee Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow GenBank Hyndman Peak 0 NA NA NA NA NA NA NA MPD Brassicales Meadow GenBank Salzburger Spitzl 0 NA NA NA NA NA NA NA MPD Brassicales Meadow GenBank Thompson Peak 1 NA NA NA NA NA NA NA MPD Brassicales Meadow GenBank D.O. Lee Peak 2 170.4198 117.9442 57.8742 7471 0.9067 0.7470 0 MPD Ericales Meadow GenBank Hyndman Peak 2 17.4979 117.6830 57.9168 819 -1.7298 0.0819 0 MPD Ericales Meadow GenBank

63 Salzburger Spitzl 1 NA NA NA NA NA NA NA MPD Ericales Meadow GenBank Thompson Peak 4 117.1612 117.1612 0.0000 5001 NA 0.5000 0 MPD Ericales Meadow GenBank Table Appendix S8: Phylogenetic β-diversity output statistics. Table of summarizing results of decomposed UniFrac index for each summit pair: observed UniFrac distance (UniFrac), observed component of UniFrac distance describing species replacement (UniFrac Turn), observed component of UniFrac distance describing species nestedness (UniFrac PD), standardized effect size (SES) comparing observed UniFrac distance to expected from shuffling the phylogeny tips (SES UniFrac), SES component of UniFrac distance describing species replacement (SES UniFrac Turn), SES component of UniFrac distance describing species nestedness (SES UniFrac PD).

All All All All All Talus: Talus: All Alpine: Alpine: Talus: Talus: Talus: Alpine: Alpine: Alpine: Talus: betadiv betadiv Alpine: betadiv betadiv betadiv betadiv betadiv Summit Pair betadiv betadiv betadiv betadiv SES SES betadiv SES SES UniFrac UniFrac SES UniFrac UniFrac SES UniFrac UniFrac UniFrac UniFrac UniFrac UniFrac turn PD UniFrac turn PD UniFrac turn PD turn PD

Braxon Peak-Castle Peak 0.6242 0.4327 0.1915 1.1690 0.6570 0.0743 0.6242 0.4327 0.1915 1.3513 0.8018 0.0677 Braxon Peak-D.O. Lee Peak 0.5636 0.4422 0.1214 -0.5223 0.2158 -0.6868 0.5863 0.5756 0.0107 0.0326 0.6051 -1.0216 Braxon Peak-Horstmann Peak 0.5500 0.3783 0.1717 0.5626 -0.5652 1.5273 0.5500 0.3783 0.1717 0.7008 -0.3966 1.3480 Braxon Peak-Hyndman Peak 0.6121 0.4167 0.1954 0.5180 1.2442 -1.1106 0.4977 0.4704 0.0274 -0.2761 0.9310 -1.4434 Braxon Peak-Mount Cramer 0.5720 0.4722 0.0998 0.3675 0.1888 0.1069 0.5720 0.4722 0.0998 0.5652 0.3950 0.0276 Braxon Peak-Salzburger Spitzl 0.6600 0.4381 0.2219 1.2066 0.8497 -0.1920 0.6205 0.4636 0.1568 1.1205 0.4893 0.3731 Braxon Peak-Snowyside Peak 0.6221 0.5323 0.0899 0.9730 0.5328 0.3710 0.6221 0.5323 0.0899 1.1784 0.7300 0.2708 Braxon Peak-Thompson Peak 0.4673 0.2873 0.1800 -0.7158 -0.6111 0.1605 0.5356 0.3608 0.1749 -0.3704 -0.6917 0.5891 Castle Peak-D.O. Lee Peak 0.5624 0.4911 0.0713 1.6757 1.1631 0.7313 0.6321 0.4265 0.2055 1.9403 0.5839 0.9378

64 Castle Peak-Horstmann Peak 0.4393 0.3840 0.0553 -1.2762 -0.3335 -0.7761 0.4393 0.3840 0.0553 -0.9033 -0.0493 -0.7980 Castle Peak-Hyndman Peak 0.4979 0.4947 0.0032 1.0745 2.0441 -1.6383 0.5332 0.3215 0.2117 2.1174 -0.0001 2.6310 Castle Peak-Mount Cramer 0.3863 0.2439 0.1424 -1.1197 -1.3334 0.6917 0.3863 0.2439 0.1424 -0.7660 -1.1437 0.7214 Castle Peak-Salzburger Spitzl 0.4401 0.3894 0.0507 -0.2877 -0.0154 -0.3343 0.4053 0.3700 0.0354 -0.5792 -0.1386 -0.5597 Castle Peak-Snowyside Peak 0.4486 0.3356 0.1130 0.6763 0.8764 -0.4344 0.4486 0.3356 0.1130 0.9172 1.0460 -0.4120 Castle Peak-Thompson Peak 0.5495 0.4871 0.0625 0.7342 0.4180 0.3937 0.6804 0.2972 0.3832 0.9478 -0.0374 0.6179 D.O. Lee Peak-Horstmann Peak 0.5977 0.5737 0.0240 0.6237 1.3093 -1.2147 0.6676 0.5405 0.1271 1.0062 -0.3184 2.2561 D.O. Lee Peak-Hyndman Peak 0.4298 0.3319 0.0979 0.2912 0.2039 0.0301 0.4679 0.4216 0.0462 0.2668 0.7665 -0.7855 D.O. Lee Peak-Mount Cramer 0.5727 0.5605 0.0122 0.4446 1.2629 -1.3179 0.6474 0.5578 0.0896 1.3628 0.7217 0.6789 D.O. Lee Peak-Salzburger Spitzl 0.4424 0.2724 0.1699 0.7761 -0.7566 2.0366 0.5531 0.3318 0.2213 1.2715 -0.3935 1.9229 D.O. Lee Peak-Snowyside Peak 0.6323 0.6260 0.0063 1.8216 2.5031 -1.6537 0.6608 0.5705 0.0902 2.5467 1.7587 0.8260 D.O. Lee Peak-Thompson Peak 0.5373 0.5279 0.0094 1.5020 1.9484 -1.0461 0.5111 0.3460 0.1651 -0.0754 0.3717 -0.5113 Horstmann Peak-Hyndman Peak 0.5410 0.5023 0.0387 0.0140 1.7736 -2.1620 0.6045 0.4934 0.1111 1.2087 0.3522 1.0680 Horstmann Peak-Mount Cramer 0.4009 0.3378 0.0631 -2.0888 -2.0812 0.4281 0.4009 0.3378 0.0631 -1.5997 -1.5836 0.3154 Horstmann Peak-Salzburger Spitzl 0.5491 0.4630 0.0861 0.4967 1.2557 -1.1469 0.5263 0.5101 0.0162 0.4676 1.0216 -1.0076 Horstmann Peak-Snowyside Peak 0.4154 0.3625 0.0530 -1.2124 -1.1830 0.1007 0.4154 0.3625 0.0530 -0.9626 -0.9303 0.0869 Horstmann Peak-Thompson Peak 0.4452 0.4214 0.0239 -1.3917 -0.4206 -0.9524 0.6690 0.3574 0.3116 0.6190 -0.8425 1.5865 Hyndman Peak-Mount Cramer 0.5308 0.4379 0.0930 0.5290 1.5473 -1.3929 0.5669 0.4958 0.0711 1.1861 0.8342 0.3686 Hyndman Peak-Salzburger Spitzl 0.3684 0.3031 0.0653 0.7940 0.5313 0.2807 0.4498 0.2388 0.2111 1.3022 -0.9776 3.7796 Hyndman Peak-Snowyside Peak 0.5909 0.5195 0.0714 1.4909 2.7717 -2.0428 0.5876 0.5154 0.0722 2.2354 1.8316 -0.0432 Hyndman Peak-Thompson Peak 0.5766 0.5224 0.0542 2.4760 2.8978 -1.3233 0.4288 0.1275 0.3013 -0.9537 0.0162 -0.7878 Mount Cramer-Salzburger Spitzl 0.5493 0.4030 0.1463 0.4331 0.2851 0.0130 0.5364 0.4734 0.0631 0.3084 0.1407 0.2185 Mount Cramer-Snowyside Peak 0.5043 0.4989 0.0054 0.5382 1.1097 -1.1827 0.5043 0.4989 0.0054 0.7020 1.3084 -1.1933 Mount Cramer-Thompson Peak 0.4811 0.4536 0.0275 0.8215 1.2984 -0.9188 0.6309 0.3564 0.2745 0.4559 -0.1593 0.5277 Salzburger Spitzl-Snowyside Peak 0.4943 0.3313 0.1631 -0.2188 0.0748 -0.2713 0.4520 0.3803 0.0717 -0.0623 0.0110 -0.0875 Salzburger Spitzl-Thompson Peak 0.6570 0.5799 0.0771 2.6603 2.1657 -0.1603 0.6445 0.2595 0.3850 1.3017 0.2504 0.6250 Snowyside Peak-Thompson Peak 0.5826 0.5667 0.0159 1.4858 1.9842 -1.2186 0.6942 0.4770 0.2172 1.7238 1.0097 0.0688 (a) Tracheophyta Asterales Poales Caryophyllales Lamiales Brassicales Ericales * * NS * NS NS NS 2 NS * NS NS NS NS NS NS NS NS NS NS NS NS

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SES MNTD High−throughput

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(b) Tracheophyta Asterales Poales Caryophyllales Lamiales Brassicales Ericales NS *** NS NS NS NS NS 2 ● NS NS * NS NS * * NS NS NS * * NS NS

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data Total Data 0 GenBank

SES MPD High−throughput

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Figure Appendix S9: Comparison of the datasets used to infer phylogenetic relationships among alpine plant species. Boxplots of standardized effect sizes (SES) for a) mean nearest taxon phylogenetic distance (MNTD) and b) mean pairwise phylogenetic distance (MPD) quantified from phylogenetic relationships that were estimated from sequence data com- bined from high-throughput sequencing and mined from GenBank (Total Data) compared with estimates from phylogenetic relationships based on data mined from GenBank or high- throughput sequences alone. Positive SES values indicate phylogenetic overdispersion (high phylogenetic divergence), negative SES indicate phylogenetic clustering (low phylogenetic divergence). The significance of a t-test comparing the means of each group is indicated about the box: p < 0.05 * , p < 0.01 **, p < 0.001 ***, NS = not significant. Boxes show first and third quartiles, solid horizontal lines indicate the median, vertical black lines are the range, and dots are points that lie 1.5x the interquartile range above the third quartile or below the first quartile. 65