DOCSLIB.ORG

Effect of a Bioactive Product SEL001 from Lactobacillus Sakei Probio65 on Gut Microbiota and Its Anti-Colitis Effects in a TNBS-Induced Colitis Mouse Model ⇑ Irfan A

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Effect of a Bioactive Product SEL001 from Lactobacillus Sakei Probio65 on Gut Microbiota and Its Anti-Colitis Effects in a TNBS-Induced Colitis Mouse Model ⇑ Irfan A Saudi Journal of Biological Sciences 27 (2020) 261–270 Contents lists available at ScienceDirect Saudi Journal of Biological Sciences journal homepage: www.sciencedirect.com Effect of a bioactive product SEL001 from Lactobacillus sakei probio65 on gut microbiota and its anti-colitis effects in a TNBS-induced colitis mouse model ⇑ Irfan A. Rather a,f, ,1, Vivek K. Bajpai a,b,1, Lew L. Ching a, Rajib Majumder a, Gyeong-Jun Nam a, ⇑ Nagaraju Indugu c, Prashant Singh d, Sanjay Kumar e, , Nahid H. Hajrah f, Jamal S.M. Sabir f, ⇑ Majid Rasool Kamli f, Yong-Ha Park a, a Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Republic of Korea b Department of Energy and Materials Engineering, Dongguk University-Seoul, 30 Pildong-ro 1-gil, Seoul 04620, Republic of Korea c Department of Clinical Studies-New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA d Department of Food Science, College of Human Science, Florida State University, Tallahassee, FL 32306, USA e Department of Poultry Science, University of Georgia, Athens, GA 30602, USA f Centre of Excellence in Bionanoscience Research, King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia article info abstract Article history: This study underpins the therapeutic potential of SEL001, a bioactive product isolated from Lactobacillus Received 29 April 2019 sakei probio65, in terms of its anti-inflammatory properties and its effect on gut-microbiota in a TNBS- Revised 25 August 2019 induced ulcerative colitis mouse model. Ulcerative colitis was developed in mice by intra rectal admin- Accepted 3 September 2019 istration of trinitrobenzene sulfonic acid. Bioactive product SEL001 (50 mg/kg b.w.) was administered Available online 4 September 2019 orally. Myeloperoxidase activity was measured using 3,30, 5,50-tetramethylbenzidine. The entire colon was sampled for post-mortem clinical assessment. Colonic injury was assessed through histological Keywords: and histomorphometric examinations. The 454 pyrosequencing and QIIME pipeline were used for gut Lactobacillus sakei probio65 microbiota analysis and statistical analysis were conducted using R. mRNA extraction from colon tissue Ulcerative colitis Anti-inflammatory effect and RT-PCR approaches were employed to determine the changes in the level of specific biomarker genes Histopathology associated with UC. The results depict that SEL001 significantly lowered pro-inflammatory cytokines, Gut-microbiota including CD4, TNF-a, and interleukin-6. Examination of clinical and histopathological traits revealed that SEL001 was effective and potent in reducing the inflammatory signatures of UC to a similar extent as did by the standard drug mesalamine (5-ASA). Pyro-sequencing 16S data revealed that the reduction in the major member of phylum Firmicutes, which has been previously associated with a higher risk of UC. The SEL001, an anti-inflammatory bioactive product originated from a probiotic strain L. sakei probio65 could be an alternative therapeutic agent for treatment of UC. Ó 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 1. Introduction one of the major challenges for gastroenterologists and World Gastroenterology Organization (WGO). The term IBD covers Inflammatory bowel disease (IBD) is a designation for the Crohn’s disease (CD) and ulcerative colitis (UC), and are known idiopathic chronic inflammatory gastrointestinal disorder, remains to affect approximately 10–20 per 100,000 people per year (Mallon et al., 2007), has a high recurrence rate and eventually lead ⇑ Corresponding authors at: Centre of Excellence in Bionanoscience Research, to high morbidity. Ulcerative colitis affects the large bowel and the King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia (I.A. Rather). associated clinical manifestations include chronic hemorrhagic E-mail addresses: [email protected] (I.A. Rather), [email protected] diarrhea, abdominal cramps and pain, gut perforation, and com- (S. Kumar), [email protected] (Y.-H. Park). plete bowel obstruction, which significantly affect the quality of 1 Both authors equally contributed. life of the ailing person. The precise etiology of the disease is not Peer review under responsibility of King Saud University. well recognized; however, host genetic factors, immune disequilib- rium, environmental factors, and host-microbial composition are believed to be responsible for the development and progression Production and hosting by Elsevier of the disease (Loftus, 2004; Hatoum and Binion, 2005; Xia et al., https://doi.org/10.1016/j.sjbs.2019.09.004 1319-562X/Ó 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). 262 I.A. Rather et al. / Saudi Journal of Biological Sciences 27 (2020) 261–270 2019; Kengo et al., 2019). Current medical interventions for 2.2. Experimental animals managing IBD are not satisfactory and does not lead to complete remission of symptoms. Further, UC patients frequently experience Colitis studies were performed in 6-wk old ICR mice (n = 30) pro- relapse and conventional treatments are not sufficient to keep cured from Samtaco Bio Co. (Osan, Korea). Mice were maintained in a remission for longer durations. Failure of standard treatments for regulated facility of 22 ± 2 °C temperature, 55 ± 10% relative humid- UC leads to colon removed in about 33–50% of cases. The front- ity and were exposed to a 12-h cycle of light and dark. Further, two line therapy for UC consists of a large derivative chain of aminos- groups of mice; control and treatment were marked, and each group alicylates, the components actively known for their anti- consisted six mice. All the mice were fed on a standard rodent diet inflammatory properties (Rousseaux et al., 2005; Sandborn, and had regular access to water. The Animal Ethical Committee of 2007). Mesalamine is a new generation formulation of 5-ASA and the Yeungnam University (Gyeongsan, Korea) approved the its clinical trials have displayed significant effectiveness for the approval for research (YNU-ANETCOMM-2015-00120). treatment of mild to moderate form of UC (Sutherland and MacDonald, 2007a, 2007b). Mesalamine along with 5-ASA lines 2.3. Preparation of SEL001 of drugs has adverse clinical side effects, sub-grouped into dose- related intolerance effects and dose-independent idiosyncratic The preparation and chemical composition of SEL001 was car- reactions. Clinical side effects of these drugs can lead to symptoms ried out by the method described in our previous study (Rather like regurgitation, nausea, headache, and abdominal pain, which et al., 2018). Briefly, Lactobacillus sakei Probio65 was cultured for are results of dose-related intolerance (Ham & Moss, 2012). 48 h in De Man, Rogosa and Sharpe (MRS) medium. Then the cul- Whereas, idiosyncratic reactions include male infertility, anemia, ture was assorted with commercial grade ethanol (1:2 ratio). Fur- hepatic and pulmonary dysfunction, hypersensitivity rash, worsen- ther, the combination was incubated at room temperature for 6 h ing colitis, renal toxicity, interstitial lung disease, cardiopulmonary with constant shaking at 150 rpm followed by centrifugation for hypersensitivity etc (Ransford and Langman, 2002; Ferrusquía 10 min centrifuged at 8000g. Finally, supernatant was carefully et al., 2015). The increased burden of the UC patients is further collected, filtered (whatman No. 1) and freeze dried. A detailed worsened by the limited spectrum UC curable by 5-ASA lines of chemical composition profile of ethanolic extract of a probiotic drugs and their alarming side effects have placed an inevitable strain L. sakei probio65 (SEL001) has been summarized in Table 2. demand for the development of broad-spectrum, effective, and safe The results are described in our previous publication (Rather et al., alternatives for the treatment of UC. 2018). The term ‘‘probiotic” refers to the administration of live microor- ganisms when taken in sufficient amount (>107 CFU/mL) have a ben- eficial effect on the host health (Plaza-Diaz et al., 2017). Also, cellular 2.4. Colitis induction in experimental mice and treatments components of these probiotic microorganisms confer health bene- fits (Matsumoto et al., 2005; Giahi et al., 2012). The probiotics are Colitis induction procedure followed in this study was similar as taken in different forms and are believed to maintain the healthy described by the Morris et al. (1989). In brief, mice were allowed to gut function (Matsumoto et al., 2005; Tojo et al., 2014). Additionally, fast for 24 h before induction of colitis and subsequent therapeutic probiotics are suggested as therapeutic mediators for gastrointesti- treatments. Mice were exposed to light anesthesia-using diethyl nal disorders and many pathologies, including inflammatory diseases ether and then exposed to the intra-rectal administration of (Plaza-Díaz et al., 2017; Tojo et al., 2014). The extract of probiotic Lac- 0.6 mg of TNBS in 0.1 ml of 30% ethanol using a trocar needle for tobacillus sakei proBio-65 has shown promising effects in treating colitis induction. A polyethylene catheter was introduced intra- imiquimod induced psoriasis like skin inflammation (Rather et al., rectally 4–5 cm into the distal part of the colon,
Load more

Recommended publications
  • The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio In
    microorganisms Review The Influence of Probiotics on the Firmicutes/Bacteroidetes Ratio in the Treatment of Obesity and Inflammatory Bowel disease Spase Stojanov 1,2, Aleš Berlec 1,2 and Borut Štrukelj 1,2,* 1 Faculty of Pharmacy, University of Ljubljana, SI-1000 Ljubljana, Slovenia; [email protected] (S.S.); [email protected] (A.B.) 2 Department of Biotechnology, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia * Correspondence: borut.strukelj@ffa.uni-lj.si Received: 16 September 2020; Accepted: 31 October 2020; Published: 1 November 2020 Abstract: The two most important bacterial phyla in the gastrointestinal tract, Firmicutes and Bacteroidetes, have gained much attention in recent years. The Firmicutes/Bacteroidetes (F/B) ratio is widely accepted to have an important influence in maintaining normal intestinal homeostasis. Increased or decreased F/B ratio is regarded as dysbiosis, whereby the former is usually observed with obesity, and the latter with inflammatory bowel disease (IBD). Probiotics as live microorganisms can confer health benefits to the host when administered in adequate amounts. There is considerable evidence of their nutritional and immunosuppressive properties including reports that elucidate the association of probiotics with the F/B ratio, obesity, and IBD. Orally administered probiotics can contribute to the restoration of dysbiotic microbiota and to the prevention of obesity or IBD. However, as the effects of different probiotics on the F/B ratio differ, selecting the appropriate species or mixture is crucial. The most commonly tested probiotics for modifying the F/B ratio and treating obesity and IBD are from the genus Lactobacillus. In this paper, we review the effects of probiotics on the F/B ratio that lead to weight loss or immunosuppression.
    [Show full text]
  • A Taxonomic Note on the Genus Lactobacillus
    Taxonomic Description template 1 A taxonomic note on the genus Lactobacillus: 2 Description of 23 novel genera, emended description 3 of the genus Lactobacillus Beijerinck 1901, and union 4 of Lactobacillaceae and Leuconostocaceae 5 Jinshui Zheng1, $, Stijn Wittouck2, $, Elisa Salvetti3, $, Charles M.A.P. Franz4, Hugh M.B. Harris5, Paola 6 Mattarelli6, Paul W. O’Toole5, Bruno Pot7, Peter Vandamme8, Jens Walter9, 10, Koichi Watanabe11, 12, 7 Sander Wuyts2, Giovanna E. Felis3, #*, Michael G. Gänzle9, 13#*, Sarah Lebeer2 # 8 '© [Jinshui Zheng, Stijn Wittouck, Elisa Salvetti, Charles M.A.P. Franz, Hugh M.B. Harris, Paola 9 Mattarelli, Paul W. O’Toole, Bruno Pot, Peter Vandamme, Jens Walter, Koichi Watanabe, Sander 10 Wuyts, Giovanna E. Felis, Michael G. Gänzle, Sarah Lebeer]. 11 The definitive peer reviewed, edited version of this article is published in International Journal of 12 Systematic and Evolutionary Microbiology, https://doi.org/10.1099/ijsem.0.004107 13 1Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology, Hubei Key 14 Laboratory of Agricultural Bioinformatics, Wuhan, Hubei, P.R. China. 15 2Research Group Environmental Ecology and Applied Microbiology, Department of Bioscience 16 Engineering, University of Antwerp, Antwerp, Belgium 17 3 Dept. of Biotechnology, University of Verona, Verona, Italy 18 4 Max Rubner‐Institut, Department of Microbiology and Biotechnology, Kiel, Germany 19 5 School of Microbiology & APC Microbiome Ireland, University College Cork, Co. Cork, Ireland 20 6 University of Bologna, Dept. of Agricultural and Food Sciences, Bologna, Italy 21 7 Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Vrije Universiteit 22 Brussel, Brussels, Belgium 23 8 Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Ghent, 24 Belgium 25 9 Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, Canada 26 10 Department of Biological Sciences, University of Alberta, Edmonton, Canada 27 11 National Taiwan University, Dept.
    [Show full text]
  • Lactobacillus Sakei 1 and Its Bacteriocin Influence
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Food Control 22 (2011) 1404e1407 Contents lists available at ScienceDirect Food Control journal homepage: www.elsevier.com/locate/foodcont Lactobacillus sakei 1 and its bacteriocin influence adhesion of Listeria monocytogenes on stainless steel surface Lizziane K. Winkelströter, Bruna C. Gomes, Marta R.S. Thomaz, Vanessa M. Souza, Elaine C.P. De Martinis* Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café s/n, 14040-903 Ribeirão Preto, São Paulo, Brazil article info abstract Article history: Listeria monocytogenes is of particular concern for the food industry due to its psychrotolerant and Received 31 August 2010 ubiquitous nature. In this work, the ability of L. monocytogenes culturable cells to adhere to stainless steel Received in revised form coupons was studied in co-culture with the bacteriocin-producing food isolate Lactobacillus sakei 1as 11 February 2011 well as in the presence of the cell-free neutralized supernatant of L. sakei 1 (CFSN-S1) containing sakacin Accepted 22 February 2011 1. Results were compared with counts obtained using a non bacteriocin-producing strain (L. sakei ATCC 15521) and its bacteriocin free supernatant (CFSN-SA). Culturable adherent L. monocytogenes and lac- Keywords: tobacilli cells were enumerated respectively on PALCAM and MRS agars at 3-h intervals for up to 12 h and Listeria monocytogenes Lactobacillus sakei after 24 and 48 h of incubation. Bacteriocin activity was evaluated by critical dilution method. After 6 h of Adhesion incubation, the number of adhered L.
    [Show full text]
  • Lactobacillus Sakei Modulates Mule Duck Microbiota in Ileum and Ceca During Overfeeding
    Lactobacillus sakei modulates mule duck microbiota in ileum and ceca during overfeeding F. Vasaï ,* K. Brugirard Ricaud ,*1 L. Cauquil ,†‡§ P. Daniel ,# C. Peillod ,Ϧ K. Gontier ,* A. Tizaoui ,¶ O. Bouchez ,** S. Combes ,†‡§ and S. Davail * * Institut pluridisciplinaire de recherche sur l’environnement et les matériaux–Equipe Environnement et Microbiologie UMR5254, IUT des Pays de l’Adour, Rue du Ruisseau, BP 201, 40004 Mont de Marsan, France; † Institut National de la Recherche Agronomique (INRA), UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France; ‡ Université de Toulouse, Institut National Polytechnique de Toulouse (INPT)–Ecole Nationale Supérieure Agronomique de Toulouse, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31326 Castanet-Tolosan, France; § Université de Toulouse INPT Ecole Nationale Vétérinaire de Toulouse, UMR1289 Tissus Animaux Nutrition Digestion Ecosystème et Métabolisme, F-31076 Toulouse, France; # Laboratoires des Pyrénées et des Landes, 1 rue Marcel David, BP219, 40004 Mont de Marsan, France; Ϧ Institut Technique de l’Aviculture, 28 rue du Rocher, 75008 Paris, France; ¶ Institut Universitaire de Technologie des Pays de l’Adour, Rue du Ruisseau, BP 201, 40004 Mont de Marsan, France; and ** Plateforme Génomique Bâtiment Centre de Ressources de Génotypage & Séquençage-Centre National de Ressources Génomiques Végétales, INRA Auzeville, Chemin de Borderouge-BP 52627, 31326 Castanet-Tolosan Cedex, France ABSTRACT The supplementation with Lactobacillus and diversity decreased in the ileum and increased in sakei as probiotic on the ileal and cecal microbiota of the ceca after overfeeding. Overfeeding increased the mule ducks during overfeeding was investigated using relative abundance of Firmicutes and especially the high-throughput 16S rRNA gene-based pyrosequenc- Lactobacillus group in ileal samples.
    [Show full text]
  • Multi-Product Lactic Acid Bacteria Fermentations: a Review
    fermentation Review Multi-Product Lactic Acid Bacteria Fermentations: A Review José Aníbal Mora-Villalobos 1 ,Jéssica Montero-Zamora 1, Natalia Barboza 2,3, Carolina Rojas-Garbanzo 3, Jessie Usaga 3, Mauricio Redondo-Solano 4, Linda Schroedter 5, Agata Olszewska-Widdrat 5 and José Pablo López-Gómez 5,* 1 National Center for Biotechnological Innovations of Costa Rica (CENIBiot), National Center of High Technology (CeNAT), San Jose 1174-1200, Costa Rica; [email protected] (J.A.M.-V.); [email protected] (J.M.-Z.) 2 Food Technology Department, University of Costa Rica (UCR), San Jose 11501-2060, Costa Rica; [email protected] 3 National Center for Food Science and Technology (CITA), University of Costa Rica (UCR), San Jose 11501-2060, Costa Rica; [email protected] (C.R.-G.); [email protected] (J.U.) 4 Research Center in Tropical Diseases (CIET) and Food Microbiology Section, Microbiology Faculty, University of Costa Rica (UCR), San Jose 11501-2060, Costa Rica; [email protected] 5 Bioengineering Department, Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), 14469 Potsdam, Germany; [email protected] (L.S.); [email protected] (A.O.-W.) * Correspondence: [email protected]; Tel.: +49-(0331)-5699-857 Received: 15 December 2019; Accepted: 4 February 2020; Published: 10 February 2020 Abstract: Industrial biotechnology is a continuously expanding field focused on the application of microorganisms to produce chemicals using renewable sources as substrates. Currently, an increasing interest in new versatile processes, able to utilize a variety of substrates to obtain diverse products, can be observed.
    [Show full text]
  • Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced with Different Curing Processes Kübra ÇİNAR 1,A Kübra FETTAHOĞLU 2,B Güzin KABAN 2,C
    Kafkas Univ Vet Fak Derg Kafkas Universitesi Veteriner Fakultesi Dergisi 25 (3): 299-303, 2019 ISSN: 1300-6045 e-ISSN: 1309-2251 Journal Home-Page: http://vetdergikafkas.org Research Article DOI: 10.9775/kvfd.2018.20853 Online Submission: http://submit.vetdergikafkas.org Genotypic Identification of Lactic Acid Bacteria in Pastirma Produced with Different Curing Processes Kübra ÇİNAR 1,a Kübra FETTAHOĞLU 2,b Güzin KABAN 2,c 1 Bayburt University, Faculty of Engineering, Department of Food Engineering, TR-69000 Bayburt - TURKEY 2 Atatürk University, Faculty of Agriculture, Department of Food Engineering, TR-25100 Erzurum - TURKEY a ORCID: 0000-0002-3715-8739; b ORCID: 0000-0002-9464-0660; c ORCID: 0000-0001-6720-7231 Article ID: KVFD-2018-20853 Received: 28.08.2018 Accepted: 04.12.2018 Published Online: 04.12.2018 How to Cite This Article Çinar K, Fettahoğlu K, Kaban G: Genotypic identification of lactic acid bacteria in pastirma produced with different curing processes. Kafkas Univ Vet Fak Derg, 25 (3): 299-303, 2019. DOI: 10.9775/kvfd.2018.20853 Abstract The lactic acid bacteria isolated from pastirma, produced under controlled conditions using two different curing temperatures (4°C or 10°C) and two different curing agents (150 mg/kg sodium nitrite or 300 mg/kg potassium nitrate), were subjected to genotypic (16S rRNA sequecing) identification. According to the identification results, 68 of 87 isolates (78.16%) was identified as Pediococcus pentosaceus. This species was followed by P. acidilactici (14.94%), Lactobacillus sakei (4.60%) and L. plantarum (2.30%), respectively. P. pentosaceus was dominant species in all curing applications (4°C/nitrate or nitrite or 10°C/nitrate or nitrite).
    [Show full text]
  • A Taxonomic Note on the Genus Lactobacillus
    TAXONOMIC DESCRIPTION Zheng et al., Int. J. Syst. Evol. Microbiol. DOI 10.1099/ijsem.0.004107 A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae Jinshui Zheng1†, Stijn Wittouck2†, Elisa Salvetti3†, Charles M.A.P. Franz4, Hugh M.B. Harris5, Paola Mattarelli6, Paul W. O’Toole5, Bruno Pot7, Peter Vandamme8, Jens Walter9,10, Koichi Watanabe11,12, Sander Wuyts2, Giovanna E. Felis3,*,†, Michael G. Gänzle9,13,*,† and Sarah Lebeer2† Abstract The genus Lactobacillus comprises 261 species (at March 2020) that are extremely diverse at phenotypic, ecological and gen- otypic levels. This study evaluated the taxonomy of Lactobacillaceae and Leuconostocaceae on the basis of whole genome sequences. Parameters that were evaluated included core genome phylogeny, (conserved) pairwise average amino acid identity, clade- specific signature genes, physiological criteria and the ecology of the organisms. Based on this polyphasic approach, we propose reclassification of the genus Lactobacillus into 25 genera including the emended genus Lactobacillus, which includes host- adapted organisms that have been referred to as the Lactobacillus delbrueckii group, Paralactobacillus and 23 novel genera for which the names Holzapfelia, Amylolactobacillus, Bombilactobacillus, Companilactobacillus, Lapidilactobacillus, Agrilactobacil- lus, Schleiferilactobacillus, Loigolactobacilus, Lacticaseibacillus, Latilactobacillus, Dellaglioa,
    [Show full text]
  • Genetic Diversity Within Lactobacillus Sakei and Primers for Its Detection
    lnternational Journal of Systematic Bacteriology (1 999), 49, 997-1 007 Printed in Great Britain Genetic diversity within Lactobacillus sakei and Lactobacillus curvatus and design of PCR primers for its detection using randomly amplified polymorphic DNA Franqoise Berthier't and Stanislav D. Ehrlich2 Author for correspondence : Franqoise Berthier. Tel : + 33 3 84 73 63 13. Fax : + 33 3 84 37 37 8 I. e-mail: berthier@,poligny.inra.fr Laboratoire de Recherches The genotypic and phenotypic diversity among isolates of the Lactobacillus sur la Viandel and curvatus/Lactobaci//usgraminisllactobacillus sakei group was evaI uated by Laboratoire de GCn6tique Microbiennez, lnstitut comparing RAPD data and results of biochemical tests, such as hydrolysis of National de la Recherche arginine, D-lactate production, melibiose and xylose fermentation, and the Agronomique, Domaine de presence of haem-dependent catalase. Analyses were applied to five type Vilvert, 78352 louy-en-Josas Cedex, France strains and to a collection of 165 isolates previously assigned to L. sakei or L. curvatus. Phenotypic and RAPD data were compared with each other and with previous DNA-DNA hybridization data. The phenotypic and genotypic separation between L. sakei, L. curvatus and L. graminis was clear, and new insights into the detailed structure within L. sakei and L. curvatus were obtained. Individual strains could be typed by RAPD and, after the elimination of similar or identical isolates, two sub-groups in both L. curvatus and L. sakei were defined. The presence or absence of catalase activity further distinguished the two L. curvatus sub-groups. By cloning and sequencing specific RAPD products, pairs of PCR primers were developed that can be used to specifically detect L.
    [Show full text]
  • Effects of a Starter Culture on Histamine Reduction, Nitrite
    bs_bs_banner Journal of Food Safety ISSN 1745-4565 EFFECTS OF A STARTER CULTURE ON HISTAMINE REDUCTION, NITRITE DEPLETION AND OXIDATIVE STABILITY OF FERMENTED SAUSAGES XINHUI WANG1,3, HONGYANG REN2, WEI WANG1 and ZHEN JIAN XIE1 1Meat-Processing Application Key Lab of Sichuan Province, School of Biological Engineering, Chengdu University, Chengdu 610106, China 2State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu, China 3Corresponding author. ABSTRACT TEL: +86-15882476156; FAX: 02884616062; A starter culture composed of Pediococcus pentosaceus, Lactobacillus sakei and EMAIL: [email protected] Staphylococcus xylosus isolated from Chinese-fermented sausages was used to manufacture fermented sausages. The effects of the starter culture on histamine Received for Publication April 21, 2015 accumulation, nitrite depletion, lipid oxidation and quality parameters including Accepted for Publication August 4, 2015 pH, water activity and total volatile base nitrogen (TVB-N), as well as the color doi: 10.1111/jfs.12227 and texture properties, were evaluated. The histamine accumulations were 0.32 and 8.85 mg/kg in fermented sausages inoculated with the starter culture and by a spontaneous fermentation, respectively. The levels of residual nitrite were 4.32 and 26.72 mg/kg in fermented sausages inoculated with the starter culture and by a spontaneous fermentation, respectively. The thiobarbituric acid reactive sub- stances test values reached up to 2.64 and 13.92 mg MDA/kg in fermented sau- sages inoculated with the starter culture and by a spontaneous fermentation, respectively. Additionally, the fermented sausages inoculated with the starter culture showed a stronger acidification and lower (P < 0.05) level of the TVB-N than that of spontaneous fermentation.
    [Show full text]
  • Lactococcus Lactis and Lactobacillus Sakei As Bio-Protective Culture to Eliminate Leuconostoc
    1 Lactococcus lactis and Lactobacillus sakei as bio-protective culture to eliminate Leuconostoc 2 mesenteroides spoilage and improve the shelf life and sensorial characteristics of commercial 3 cooked bacon 4 5 Giuseppe Comi, Debbie Andyanto, Marisa Manzano and Lucilla Iacumin* 6 7 Department of Food Science, University of Udine, Via Sondrio 2/A, 33100 Udine, Italy. 8 9 Running headline: Quality improvement of cooked bacon. 10 11 *Correponding author: 12 Lucilla Iacumin, PhD 13 Dipartimento di Scienze degli Alimenti, Università degli Studi di Udine 14 Via Sondrio 2/A, 33100 Udine, Italy 15 e-mail: [email protected]; 16 Phone: +39 0432 558126; 17 Fax. +39 0432 558130. 18 19 20 21 22 23 24 25 26 1 27 28 Abstract 29 Cooked bacon is a typical Italian meat product. After production, cooked bacon is stored at 4 ± 2 30 °C. During storage, the microorganisms that survived pasteurisation can grow and produce spoilage. 31 For the first time, we studied the cause of the deterioration in spoiled cooked bacon compared to 32 unspoiled samples. Moreover, the use of bio-protective cultures to improve the quality of the 33 product and eliminate the risk of spoilage was tested. The results show that Leuconostoc 34 mesenteroides is responsible for spoilage and produces a greening colour of the meat, slime and 35 various compounds that result from the fermentation of sugars and the degradation of nitrogen 36 compounds.. Finally, Lactococcus lactis spp. lactis and Lactobacillus sakei were able to reduce the 37 risk of Leuconostoc mesenteroides spoilage. 38 39 40 Keywords: Cooked bacon, spoilage, bio-protective cultures.
    [Show full text]
  • From Cheese Whey Permeate to Sakacin A: a Circular Economy Approach for the Food-Grade Biotechnological Production of an Anti-Listeria Bacteriocin
    biomolecules Article From Cheese Whey Permeate to Sakacin A: A Circular Economy Approach for the Food-Grade Biotechnological Production of an Anti-Listeria Bacteriocin Alida Musatti 1,* , Daniele Cavicchioli 2 , Chiara Mapelli 1, Danilo Bertoni 2 , Johannes A. Hogenboom 1, Luisa Pellegrino 1 and Manuela Rollini 1 1 DeFENS, Department of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy; [email protected] (C.M.); [email protected] (J.A.H.); [email protected] (L.P.); [email protected] (M.R.) 2 ESP, Department of Environmental Science and Policy, Università degli Studi di Milano, Via G. Celoria 2, 20133 Milano, Italy; [email protected] (D.C.); [email protected] (D.B.) * Correspondence: [email protected]; Tel.: +39-025-031-9150 Received: 25 February 2020; Accepted: 10 April 2020; Published: 12 April 2020 Abstract: Cheese Whey Permeate (CWP) is the by-product of whey ultrafiltration for protein recovery. It is highly perishable with substantial disposal costs and has serious environmental impact. The aim of the present study was to develop a novel and cheap CWP-based culture medium for Lactobacillus sakei to produce the food-grade sakacin A, a bacteriocin exhibiting a specific antilisterial activity. Growth conditions, nutrient supplementation and bacteriocin yield were optimized through an experimental design in which the standard medium de Man, Rogosa and Sharpe (MRS) was taken as benchmark. The most convenient formulation was liquid CWP supplemented with meat extract (4 g/L) and yeast extract (8 g/L). Although, arginine (0.5 g/L) among free amino acids was depleted in all conditions, its supplementation did not increase process yield.
    [Show full text]
  • Taxonomy of Lactobacilli and Bifidobacteria
    Curr. Issues Intestinal Microbiol. 8: 44–61. Online journal at www.ciim.net Taxonomy of Lactobacilli and Bifdobacteria Giovanna E. Felis and Franco Dellaglio*† of carbohydrates. The genus Bifdobacterium, even Dipartimento Scientifco e Tecnologico, Facoltà di Scienze if traditionally listed among LAB, is only poorly MM. FF. NN., Università degli Studi di Verona, Strada le phylogenetically related to genuine LAB and its species Grazie 15, 37134 Verona, Italy use a metabolic pathway for the degradation of hexoses different from those described for ‘genuine’ LAB. Abstract The interest in what lactobacilli and bifdobacteria are Genera Lactobacillus and Bifdobacterium include a able to do must consider the investigation of who they large number of species and strains exhibiting important are. properties in an applied context, especially in the area of Before reviewing the taxonomy of those two food and probiotics. An updated list of species belonging genera, some basic terms and concepts and preliminary to those two genera, their phylogenetic relationships and considerations concerning bacterial systematics need to other relevant taxonomic information are reviewed in this be introduced: they are required for readers who are not paper. familiar with taxonomy to gain a deep understanding of The conventional nature of taxonomy is explained the diffculties in obtaining a clear taxonomic scheme for and some basic concepts and terms will be presented for the bacteria under analysis. readers not familiar with this important and fast-evolving area,
    [Show full text]