21.10.2014HIG1 antibody (pAb)

Rabbit Anti-Human Hypoxia-inducible 1 (HIGD1A, HSPC010)

Instruction Manual

Catalog Number PK-AB718-6489 Synonyms HIG1 Antibody: Hypoxia-inducible gene 1 protein, HIGD1A, hypoxia inducible domain family 1A, HSPC010 Description HIG1 and HIG2 (Hypoxia-inducible gene 1 and 2, respectively) are known to be induced by hypoxic conditions. HIG1 is induced by hypoxia and by deprivation in cultured cells. In addition, tumor xenografts derived from human cervical cells display increased expression of HIG1 and HIG2 when they are deprived of oxygen. Unlike HIG2, which is ubiquitously expressed and might be an activator and target of the canonical Wnt pathway, the function and the mechanisms underlying its regulation of HIG1 still remained unknown. The putative link between hypoxia and an oncogenic signaling pathway might play an important role in tumorigenesis. Quantity 100 µg Source / Host Rabbit Immunogen HIG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human HIG1. Purification Method Affinity chromatography purified via peptide column. Clone / IgG Subtype Polyclonal antibody Species Reactivity Human Specificity HIG1 antibody is predicted to not cross-react with HIG2 Formulation Antibody is supplied in PBS containing 0.02% sodium azide. Reconstitution During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Storage & Stability Antibody can be stored at 4ºC for three months and at -20°C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Applications E, WB, ICC, IF |Note: Antibody might be suitable for other applications not tested so far. Optimal concentrations for each application have to be determined indivudually. HIG1 antibody can be used for detection of HIG1 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 µg/mL. For immunofluorescence start at 10 µg/mL. Images Available upon request. References Bedo G, Vargas M, Ferreiro MJ, et al. Characterization of hypoxia induced gene 1: expression during rat central nervous system maturation and evidence of antisense RNA expression. Int. J. Dev. Biol. 2005; 49:431-6. Simpson JC, Wellenreuther R, Poustka A, et al. Systematic subcellular localization of novel identified by large-scale cDNA sequencing. EMBO Rep. 2000; 1:287-92. Denko NC, Schindler C, Koong A, et al. Epigenetic regulation of in cervical cancer cells by the tumor microenvironment. Clin. Cancer Res. 2000; 6:480-7. Gimm T, Wiese M, Teschemacher B, et al. Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1. FASEB J. 2010; 24:4443-58. Images Available upon request. Related Products Cat. No. PK-AB718-6489 - HIG1 Peptide Cat. No. PK-AB718-1210 - 293 Lysate

FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PROCEDURES.

PromoCell GmbH North America 1 – 866 – 251 – 2860 (toll free) Email: [email protected] Sickingenstr. 63/65 Deutschland 0800 – 776 66 23 (gebührenfrei) www.promokine.info 69126 Heidelberg France 0800 90 93 32 (ligne verte) Germany United Kingdom 0800 – 96 03 33 (toll free) Other Countries +49 6221 – 649 34 0 09/2014