Table S1. The bacterial and fungal isolates used for the in vitro activity testing.

KTU-286 Microorganism Strain No Feature MIC (µg/mL) Acinetobacter baummanii ATCC 17978 Reference strain 128 < Aspergillus flavus 12B Clinical isolate 128 < Aspergillus fumigatus 14 Clinical isolate 128 < Candida albicans ATCC 10231 Type strain 128 < Candida auris CAU-1 Clinical isolate 128 < Cuninghamella corymbifera CC1 Clinical isolate 128 < Escherichia coli 10025 Mcr-1 128 Escherichia coli DH5a Pan-S 128 Klebsiella pneumoniae 3122 blaKPC 128 < Klebsiella pneumoniae 4141 blaNDM-1 128 < Mycobacterium abscesus IP-K01 Clinical isolate 64 Mycobacterium bovis BCG Type strain 128 < Mycobacterium smegmatis mc2155 Type strain 128 < Pseudomonas aeruginosa 3691 AmpC 128 < Pseudomonas aeruginosa 3656 Pan-S 128 < Pseudomonas aeruginosa 3647 Efflux pump overexpression 128 < Pseudomonas aeruginosa 3619 OPRD 128 < Staphylococcus aureus 875 MRSA 1

Table S2. The In silico predictions of human proteins interacting with KTU-286. The in silico predictions were computed using SwissTargetPrediction tool.

Common Uniprot ChEMBL Probab Identified target Target Class name ID ID ility CHEMBL2 0.10057 Arachidonate 5-lipoxygenase ALOX5 P09917 Oxidoreductase 15 8902 CHEMBL5 0.10057 Prostaglandin E synthase PTGES O14684 Enzyme 658 8902 CHEMBL2 Family A G protein- 0.10057 Interleukin-8 receptor B CXCR2 P25025 434 coupled receptor 8902 CHEMBL2 0.10057 Cyclooxygenase-2 PTGS2 P35354 Oxidoreductase 30 8902 CHEMBL3 0.10057 Carboxylesterase 2 CES2 O00748 Enzyme 180 8902 CHEMBL5 0.10057 Poly [ADP-ribose] polymerase 2 PARP2 Q9UGN5 Enzyme 366 8902 CHEMBL6 0.10057 Tankyrase-2 TNKS2 Q9H2K2 Enzyme 154 8902 CHEMBL6 0.10057 Tankyrase-1 TNKS O95271 Enzyme 164 8902 Trace amine-associated receptor CHEMBL5 Family A G protein- 0.10057 TAAR1 Q96RJ0 1 (by homology) 857 coupled receptor 8902 Death-associated protein kinase CHEMBL2 0.10057 DAPK3 O43293 Kinase 3 468 8902 CHEMBL2 0.10057 Carbonic anhydrase II CA2 P00918 Lyase 05 8902 CHEMBL2 0.10057 Carbonic anhydrase I CA1 P00915 Lyase 61 8902 CHEMBL3 0.10057 Carbonic anhydrase IX CA9 Q16790 Lyase 594 8902

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Epidermal growth factor CHEMBL2 0.10057 EGFR P00533 Kinase receptor erbB1 03 8902 Acyl coenzyme A:cholesterol CHEMBL2 0.10057 CES1 P23141 Enzyme acyltransferase 265 8902 CHEMBL1 0.10057 Phosphomannomutase 2 PMM2 O15305 Enzyme 741162 8902 Cyclin-dependent kinase CDK5R1 Q15078 CHEMBL1 0.10057 Kinase 5/CDK5 activator 1 CDK5 Q00535 907600 8902 Cyclin-dependent kinase CCNE1 P24864 CHEMBL1 0.10057 Kinase 2/cyclin E1 CDK2 P24941 907605 8902 CHEMBL2 0.10057 Mannose-6-phosphate isomerase MPI P34949 Isomerase 758 8902 CHEMBL3 0.10057 Glutathione S-transferase Pi GSTP1 P09211 Enzyme 902 8902 CHEMBL4 0.10057 Phospholipase C-gamma-2 PLCG2 P16885 Hydrolase 100 8902 CHEMBL4 0.10057 Glutathione S-transferase Mu 2 GSTM2 P28161 Enzyme 589 8902 Phosphoethanolamine/phosphoc PHOSPH CHEMBL6 0.10057 Q8TCT1 Enzyme holine phosphatase O1 113 8902 CHEMBL2 0.10057 Leukocyte elastase ELANE P08246 Protease 48 8902 CHEMBL2 0.10057 Cathepsin D CTSD P07339 Protease 581 8902 CHEMBL2 Family A G protein- 0.10057 Adenosine A3 receptor ADORA3 P0DMS8 56 coupled receptor 8902 CHEMBL2 0.10057 Thrombin F2 P00734 Protease 04 8902 CHEMBL4 0.10057 Complement C1r C1R P00736 Protease 611 8902 CHEMBL4 0.10057 Beta-chymotrypsin CTRB1 P17538 Protease 796 8902 CHEMBL2 0.10057 Glycogen synthase kinase-3 beta GSK3B P49841 Kinase 62 8902 CHEMBL5 Toll-like and Il-1 0.10057 Toll-like receptor (TLR7/TLR9) TLR9 Q9NR96 804 receptors 8902

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