NEDD4L monoclonal antibody (M04), clone 1D2

Catalog # : H00023327-M04 規格 : [ 100 ug ] List All

Specification Application Image

Product Mouse monoclonal antibody raised against a partial recombinant Western Blot (Transfected lysate) Description: NEDD4L.

Immunogen: NEDD4L (AAH32597, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence: MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLY VADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLT enlarge

Host: Mouse Western Blot (Recombinant protein) Reactivity: Human Immunoprecipitation Isotype: IgG2a Kappa

Quality Control Antibody Reactive Against Recombinant Protein. Testing:

enlarge

Sandwich ELISA (Recombinant protein)

enlarge Western Blot detection against Immunogen (36.63 KDa) . ELISA Storage Buffer: In 1x PBS, pH 7.4

Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. Instruction:

MSDS: Download

Datasheet: Download

Applications

Western Blot (Transfected lysate)

Page 1 of 3 2019/2/4 Western Blot analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2.

Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate. Protocol Download

Western Blot (Recombinant protein) Protocol Download

Immunoprecipitation

Immunoprecipitation of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NEDD4L monoclonal antibody. Protocol Download

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody. Protocol Download

ELISA

Gene Information

Entrez GeneID: 23327

GeneBank BC032597 Accession#:

Protein AAH32597 Accession#: Page 2 of 3 2019/2/4 Gene Name: NEDD4L

Gene Alias: FLJ33870,KIAA0439,NEDD4-2,RSP5,hNedd4-2

Gene neural precursor cell expressed, developmentally down-regulated 4-like Description:

Omim ID: 606384

Gene Ontology: Hyperlink

Other neural precursor cell expressed, developmentally down-regulated gene Designations: 4-like,ubiquitin-protein ligase NEDD4-like,ubiquitin-protein ligase Rsp5

Gene Pathway Endocytosis Ubiquitin mediated proteolysis

Related Disease Genetic Predisposition to Disease Hypertension Hypotension, Orthostatic Tobacco Use Disorder

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