variability of the expression. Consistent with this idea, the proliferation in vitro, under nutritional stress, the presence of exogenous hormones (auxins and cytokinins) and other factors exploit the same multiply mechanism that was established in vivo, during the evolution of hypertrophy and hyperthely. Bibliography 1. Colesnicova L.S., Matienco B.T. Investigation of particularities of binucleation in pericarp cells of old apple cultivars. Rev. of AS of RM, Biol.and chem.., 1994, 1: 30-32 2. Marinescu M., Matienco B. Cytological analysis of tomato fruit callus culture. Proc. VIII Intern. Congr. Plant Cell Culture. Firenze, 12-17 June, 1994: 266. 3. Matienco B. Comparative anatomy and ultrastructure of Cucurbita fruits. Kishinev: Cartea Moldoveneasca, 1969: 407 p. 4. Matienco B., Maximova E., Brezeanu A., Marinescu M. Visualization of intracellular traffi c of phenolic compounds during its biosynthesis and translocation in grape berry callus. Proc. 13th Nat. Electron Microsc. Congr. Ankara, 1-4 Sept., 1997: 167-173. 5. Rumyantseva N.I., Valyeva A.I. et al. Peculiarities of lignifi cation of cell walls of buckwheat calli with different morphogenic ability. Tsitologiya (in Russian), 1998, 40: 835-843. EVALUATION OF GENETIC DIVERSITY OF ORIGANUM GENUS SPECIES Martea Rodica, Mutu Ana, Clapco Steliana, Budeanu Oleg, Duca Maria University of the Academy of Sciences of Moldova Introduction Origanum belonging to the Lamiaceae family is one the most wide-spread genus in the spontaneous fl ora of many landscapes. It has a precious natural resource, as they provide raw material for pharmaceutical industry, modern and traditional forms of medicine and generate employment and income in addition to conservation of biodiversity [1]. The genus includes 43 species of perennial and annual plants, which grow on rocky slopes, at a wide range of altitudes [1, 2, 3]. The aerial part of the plant is often used directly while the extracted essential oils exhibit antimicrobial properties, cytotoxic and antioxidant activities [5]. Taxonomic studies based on morphological characters [3] and on the results of biochemical analysis of the essential oils and their constituent compounds [5] indicate a high degree of variability across species of the Origanum genus. In light of these results, we considered signifi cant to analyze the genetic polymorphism in two species of the Origanum genus - Origanum vulgare L. and Origanum laevigatum Boiss., collected from a region of spontaneous fl ora in Moldova (Orheiul-Vechi). Material and methods The plant material was used in the form of dry leaves. Total genomic DNA was isolated from fully developed leaves using the CTAB extraction protocol [4], by 23 gradually increasing the liquid nitrogen concentration. A total of 8 single decamer random oligonucleotide primers of arbitrary sequence (OPA2, OPA9, OPA19, OPB01, OPG05, OPK17 şi Oligo391) were tested for PCR amplifi cation. DNA amplifi cation according to a basic PCR protocol was performed in a total volume of 15 μL, containing 50 ng of template DNA, 0,4 - 0,6 μM of single primer, 1 U Taq DNA polymerase 200 μM of each dNTP, 2,5 mM MgCl2 [6]. Results and discussions The RAPD-PCR analysis of the Origanum sp. genotypes indicated a high heterogeneity of the results by genotype and tested primers. Based on the electrophoretic spectrum, 82 amplifi cation products were identifi ed with an average of 11,71 bands per primer. The number of amplifi ed fragments varied in the range from 0 to 12 depending on the primer used. The comparative analysis of molecular polymorphism across the named species revealed that the RAPD-PCR generated 47 bands for Origanum vulgare L. and 35 for Origanum laevigatum Boiss. 23 of them are polymorphic and 35 – monomorphic fragments, 22 for O. vulgare L., respectively 13 fragments for O. laevigatum Boiss. The Oligo391 decamer presented six bands for both species, all monomorphic. The obtained results allowed concluding that the most informative primers are: for O. vulgare L. - UBC250 and for O. laevigatum Boiss. - OPK17 showing a maximum number of fragments (12, respectively 9 bands). OPG5 (O. vulgare L.) and UBC250 (O. laevigatum Boiss.) decamers did not generate any amplicons. The most specifi c bands were revealed in the case of OPA02 (O. laevigatum Boiss. - 4) and UBC250 (O. vulgare L. - 12) decamers. Conclusions RAPD-PCR analysis has established a high heterogeneity between Origanum vulgare L. and Origanum laevigatum Boiss. Specifi c particularities of each species depend by tested primers. The most primers showed a high degree of molecular polimorfi sm. The most informative primers are UBC250 for O. vulgare L. and OPK17 for O. laevigatum Boiss.). These studies are the part of Moldova-Romanian project “Genetic intraspecifi c polymorfysm analysis for the elaboration of molecular markers of some medicinal and aromatic plants chemotypes”. Bibliogrphy 1. Cantino, P.D., Harley, R.M., Wagstaff, S.J. Genera of Labiatae: Status and classifi cation. In:Harley, R. M., Reynolds, T. (eds.) Advances in Labiate Science. Royal Botanic Gardens Kew, Richmond, Surrey, UK, 1992. 2. Danin, A., Künne, I. Origanum jordanicum (Labiatae), a new species from Jordan, and notes on other species of sect. Campanulaticalyx, Willdenowia, 1996, 25: 601-611. 3. Ietswaart, J.H. A taxonomic revision of the genus Origanum (Labiatae). Leiden Botanical Series 4, Leiden University Press, The Hague, 1980. 4. Murray, M.G., Thompson, W.F. Rapid isolation of high molecular weight plant DNA, Nucleic Acids Res. 1980, 8,(19):4321-4325. 24 5. Padulosi, S. Oregano. Promoting the conservation and use of underutilized and neglected crops. Proceedings of the IPGRI International Workshop on Oregano, 8-12 May 1996, CIHEAM, Valenzano (Bari), Italy. Institute, ISBN 92-9043-317-5. 6. Williams, J.G.K., Kubelik, A.R., Livak, K.J., et al. DNA polymorphisms amplifi ed by arbitrary primers are useful as genetic markers, Nucl. Acids Res, 1990, vol. 18, nr. 22. p. 6531-6535. THE DEGREE OF ACCUMULATION OF THE RADIONUCLIDE Cs-137 IN SPONTANEOUS VEGETATION Motelica L., Stegărescu V. Institute of Ecology and Geography of ASM Introduction Natural and artifi cial radioactive elements have become vital component parts of environmental pollution. The main source of soil pollution with artifi cial radionuclides and with nuclear decay products has become the global rainfall as a result of nuclear experiments and the impact of Chernobyl Atomic Power Station. In the natural ecosystems and those anthropogenic ones some radionuclides (Cs- 137,Sr-90) are taken over very well especially by the plants that are mentioned in the special literature as bioindicators of environmental radioactivity [1]. For the evaluation of radionuclide transfer processes from the soil in the plant there are used different indices, one of them being the coeffi cient of accumulation (Kac.) [2]. Material and methods As an object of study served the medicinal plants-stinging nettle (Urtica dioca L.) and celandine (Chelidonium majus L.) in Landscape Reserve (LR) Holoşnita and liquorice milkvetch (Astragalus glycyphyllos L.) and moss (Hylocomium splendens) in LR Cosauti, with reduced accumulation of radionuclides from soil [1]. In order to determine radiospectrometrically the content of the radionuclides Cs-137 [3], there were taken samples of ground and vegetation. The accumulation/ transfer degree of the radionuclide was calculated according to the formula: Kac. = Cpl./CSol, where Cpl. – stands for the radionuclide concentration in the plant (Bq/kg), and CSol – radionuclide concentration in the soil (Bq/kg) [2]. Results and discussions Radiospectrometrical analysis of soil samples revealed for the LR Cosui a quantity of 13.37 Bq/kg (leached chernozem soil) and for the LR Holoşnita – 30.52 Bq/kg (uneroded leached chernozem soil). The highest concentration of the radionuclide Cs-137 (16.0 Bq/kg) was observed in moss (Hylocomium splendens) in the LR Cosui compared to 3.0 Bq/kg in wild licorice. In the plants of Urtica dioca L. and Chelidonium majus L. in the LR Holoşnita the concentration of Cs 137 constituted 6.0 Bq/kg. The calculation of storage/transfer coeffi cient of Cs- 137 from the soil of protected areas into the plants revealed the following: for Chelidonium majus L. and Urtica dioca L. – 0.19, Astragalus glycyphyllos L. – 1.22 and Hylocomium splendens – 1.19. According to the Basic Standards of 25 Powered by TCPDF (www.tcpdf.org).