Patch Test Concentrations and Vehicles for Testing Contact Allergens

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49_907_928 05.11.2005 12:13 Uhr Seite 907

Chapter 49 Patch Test Concentrations 49 and Vehicles for Testing Contact Allergens

Anton C. De Groot, Peter J. Frosch

Patch testing is a relatively safe and reasonably reli- Guidelines for testing the patient’s own contact able method for identifying contact allergens in pa- materials are provided in Chap. 50. tients with contact dermatitis. It has been clearly Table 2 lists alphabetically all chemicals men- shown that patch testing is necessary in the majority tioned in this book with their test concentrations and of patients with eczema [1]. The technique of patch vehicles (sometimes two concentrations are suggest- testing is described in Chap. 22. ed when insufficient data are available) as suggested All patients are tested with the European standard by the various authors. All allergens commercially series,containing the most frequent contact allergens available are also listed with their supplier(s), their in European countries (Table 1). Often, standard se- test concentrations, and vehicles as supplied. It ries patch testing is not enough, and additional aller- should be appreciated that for a considerable num- gens or potential allergens need to be tested, based ber of allergens, the concentrations vary between on the patient’s history and clinical examination. Ex- suppliers. Table 3 provides a list of test concentra- amples are products and chemicals to which the pa- tions for groups of chemicals as suggested by various tient is exposed occupationally or in his or her home authors in this book. Table 4 finally is an alphabetical environment. Test series containing the most fre- listing of commonly used abbreviations and their full quent allergens in certain products (preservatives, chemical synonyms. fragrances, dental materials, plastics and glues, medicaments) or in certain occupations (hairdressing, pesticides, oil and cooling fluid) are very helpful.Ap- References proximately 510 patch test materials are commercially available from Hermal (Reinbek, Germany, www. 1. Rycroft RJG (1990) Is patch testing necessary? In: hermal.de), Chemotechnique Diagnostics (Malmö, Champion RH, Pye RJ (eds) Recent advances in dermatology, vol 8. Churchill Livingstone, Edinburgh, pp 101–111 Sweden, www.chemotechnique.se) and Brial Allergen 2. De Groot AC (1994) Patch testing. Test concentrations and (Greven, Germany, www.brial.com). vehicles for 3700 chemicals, 2nd edn. Elsevier, Amsterdam For other chemicals and products, the investigator 3. Rietschel RL, Fowler JF Jr (eds) (1995) Fisher’s contact der- must decide how to apply them as a patch test.Chem- matitis, 4th edn. Williams and Wilkins, Baltimore, Md. icals usually need to be diluted,and it is of the utmost 4. Adams RM (1990) Occupational skin disease, 2nd edn. Saunders, Philadelphia, Pa. importance to use an appropriate patch test concen- 5. De Groot AC, Weijland JW, Nater JP (1994) Unwanted tration and vehicle to avoid both false-negative and effects of cosmetics and drugs used in dermatology, 3rd false-positive (irritant) reactions. The most useful edn. Elsevier, Amsterdam reference source for documented test concentrations 6. Kanerva L, Elsner P,Wahlberg JE, Maibach HI (eds) (2000) Handbook of occupational dermatology. Springer, Berlin and vehicles of chemicals, groups of chemicals and Heidelberg New York products is the book Patch testing. Test concentrations and vehicles for 3700 chemicals [2]. Other useful lists are provided in recent textbooks on contact dermatitis [3–6]. 49_907_928 05.11.2005 12:13 Uhr Seite 908

908 Anton C. De Groot, Peter J. Frosch

Table 1. The European standard series

Chemical Test concentration and vehicle

Metals Cobalt chloride 1% pet. Nickel sulfate 5% pet. Potassium dichromate 0.5% pet.

Rubber chemicals Thiuram mix 1% pet. Dipentamethylenethiuram disulfide (0.25%) Tetramethylthiuram disulfide (0.25%) 49 Tetraethylthiuram disulfide (0.25%) Tetramethylthiuram monosulfide (0.25%) N-Isopropyl-N´-phenyl-p-phenylenediamine 0.1% pet. Mercapto mix 2% pet. N-Cyclohexylbenzothiazyl sulfenamide (0.5%) Dibenzothiazyl disulfide (0.5%) Mercaptobenzothiazole (0.5%) Morpholinyl mercaptobenzothiazole (0.5%) Mercaptobenzothiazole 2% pet.

Medicaments Budesonide 0.01% pet. Benzocaine 5% pet. Neomycin sulfate 20% pet. Clioquinol® 5% pet. Tixocortol pivalate 0.1% pet.

Cosmetic ingredients Balsam of Peru (Myroxylon pereirae) 25% pet. 5-Chloro-2-methylisothiazol-3-one/2-methylisothiazol-3-one (MCI/MI) 0.01% aq. Colophonium (rosin) 20% pet. Formaldehyde 1% aq. Fragrance mix (incl. 5% sorbitan sesquioleate) 8% pet. α-Amylcinnamaldehyde (1%) Cinnamal(dehyde) (1%) Cinnamyl alcohol (1%) Eugenol (1%) Geraniol (1%) Hydroxycitronellal (1%) Iso-eugenol (1%) Oak moss absolute (Evernia prunastri) (1%) Paraben mix 16% pet. Butylparaben (4%) Ethylparaben (4%) Methylparaben (4%) Propylparaben (4%) p-Phenylenediamine free base 1% pet. Quaternium-15 1% pet. Wool wax alcohols (lanolin alcohol) 30% pet. Hydroxymethylpentylcyclohexenecarboxaldehyde (Lyral®) 5% pet.

Miscellaneous p-tert-Butylphenol formaldehyde resin 1% pet. Epoxy resin 1% pet. Primin 0.01% pet. Sesquiterpene lactone mix 0.1% pet. Alantolactone (0.033%) Dehydrocostus lactone (0.033%) Costunolide (0.033%) 49_907_928 05.11.2005 12:13 Uhr Seite 909

Patch Test Concentrations and Vehicles Chapter 49 909

Table 2. Test concentrations, vehicles and commercial availability of contact allergens. [Alc. Alcohol, aq. water, DMSO dimethyl sulfoxide, Glyc. glycerine, MEK methyl ethyl ketone (butanone), o.o. olive oil, pet. petrolatum, prop. glyc. propylene glycol.] [icr Immediate contact reactions reported (Chaps 5 and 26), de drug eruption with positive patch test reported (Chap. 24), fde fixed drug eruption with positive patch test reported (Chap. 24), ph photosensitivity reported (Chaps 6, 17 and 27), phde photosensitive drug eruption with positive photopatch test reported (Chaps 6, 17, 24 and 27)]

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Abietic acid (icr) 10% pet. + + + Acebutolol hydrochloride 2% pet. Aceclofenac 1% pet Acetylsalicylic acid (icr) 10% pet. Achillea millefolium (yarrow extract) 1% pet. + + Acid black 48 (CI 65005) 1% pet. Acid red 14 (azorubine) 0.1% alc. Acid red 118 (CI 26410) 5% pet. + Acid red 359 5% pet. + Acid violet 17 (CI 42650) 1% pet. Acid yellow 36 (CI 13065, metanil yellow) 1% pet. + + + Acid yellow 61 (CI 18968) 5% pet. Actarit (phde) 1% pet Aciclovir (fde) 5% pet. Alantolactone (icr) 0.33% pet. Alclometasone-17,21-dipropionate 1% alc. 1% pet. Alcohol, ethyl (icr) 10% aq. Alimemazine tartrate see Trimeprazine tartrate Allantoin 0.5% aq. Althiazide (phde) 10% pet. or aq. Aluminum (icr) Pure Aluminum chloride hexahydrate 2% pet. Allylisopropylacetylurea See Apronalide Amalgam 5% pet. Amalgam alloying metals 20% pet. + + Amalgam non gamma 2 5% pet. Amcinonide 1% alc. 0.1% pet. 0.1% pet. Amerchol ® L-101 See Lanolin alcohol and paraffinum liquidum Amethocaine See Tetracaine hydrochloride Amikacin sulfate 20% pet. 4-Aminoantipyrine See Ampyrone p-Aminoazobenzene (Solvent yellow 1, CI 11000) 0.25% pet 1% pet. + 1% pet. ε-Aminocaproic acid 1% aq. Amino-4-N, N-diethylaniline sulfate (TSS Agfa®) 1% pet. 2-2-(Aminoethoxy)ethanol See Diglycolamine Amino-4-N-ethyl-N-(methanesulfon- 1% pet. + + aminoethyl)-m-toluidine (CD 3) Aminoglycosides (de) 20% pet. m-Aminophenol 1% pet. + + + p-Aminophenol (CI 76550) (icr) 1% pet. + + + Aminophylline (fde) 10% pet. Amitriptyline (phde) 5% pet. Amlexanox (fde) 1% pet. Ammoniated mercury 1% pet. + + + Ammonium bituminosulfonate See Ichthammol Ammonium heptamolybdate 1% aq. Ammonium hexachloroplatinate (icr) 0.1% aq. + Ammonium persulfate (icr) 2.5% pet. + + + Ammonium tetrachloroplatinate (icr) 0.25% pet. + 0.25% aq. + Ammonium thioglycolate 1% pet., fresh 2.5% aq. 1% pet. Amorolfine 1% pet. Ampicillin (icr) 5% pet. + Ampiroxicam (phde) 1% pet. Amprolium hydrochloride 10% aq. Ampyrone (4-aminoantipyrine) (icr) 10% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 910

910 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

α-Amylcinnamaldehyde (icr) 2% pet. 1% pet. + 1% pet. Amylocaine hydrochloride 5% pet + Anethole 5% pet. Aniline 1% pet. + Antazoline 1% pet. Anthemis nobilis (chamomilla romana) 1% pet. Antipyrine (phenazone) (de) 5% pet. 49 Apronalide (allylisopropylacetylurea) (fed) 5% pet. Arnica montana (arnica extract) 0.5% pet. + + Arsanilic acid 10% pet. Atenolol (de) 10% pet. Atranorin (ph) 0.1% pet. Atropine sulfate 1% aq. or pet + (aq.) + (aq.) Azathioprine 1% pet. Azidamfenicol 2% pet. Azodicarbonamide 0.5% pet. Azodiisobutyrodinitrile 1% pet. Azorubine See Acid red 14 Bacitracin (icr) 20% pet. + 5% pet. + Bacitracin zinc 20% pet. Balsam of Peru See Myroxylon pereirae Balsam of Tolu See Myroxylon toluiferum Basic brown 1 See Bismarck Brown R Basic red 46 1% pet. Beech tar See Fagus sylvatica Befunolol 1% aq. Benomyl 0.1–1% pet. Benoxinate See Oxybuprocaine Benzaldehyde (icr) 5% pet. + + Benzalkonium chloride (BAK) 0.01–0.1% aq. 0.1% pet. 0.1% aq. 0.1% pet. Benzamine lactate 1% pet. 2-Benzimidazolethiol 1% pet. (2-mercaptobenzimidazole) Benzisothiazolinone (BIT) (ph) 0.1% pet. + 0.05% pet. + Benzocaine (icr) (ph) 5% pet. + + + Benzodiazepines (de) 1–5% aq. or pet. Benzoic acid (icr) (ph) 5% pet. 5% pet.; 1% alc./glyc. Benzoin resin see Styrax benzoin Benzophenone-3 (oxybenzone) (ph) 10% pet. + + + Benzophenone-4 (sulisobenzone) (ph) (icr) 10% pet. + + + Benzophenone-10 (mexenone) (ph) 10% pet. + IH-Benzotriazole 1% pet. + + + Benzyl benzoate 5% pet. Benzoyl peroxide (icr) 1% pet. + + + Benzydamine hydrochloride (ph) 5% pet. or aq. Benzyl alcohol (icr) 1% pet. + + + Benzyl cinnamate 5% pet. Benzylhemiformal 1% pet. Benzyl salicylate 1–5% pet. 1% pet. 2% pet. 1% pet. Bergamot oil See Citrus bergamia Beryllium chloride or sulfate 1% pet. Betamethasone dipropionate 1% alc. Betamethasone-17-valerate 1% alc. 0.12% pet. 1% pet. 0.12% pet. Betaxolol hydrochloride 1% aq. Betula alba (birch tar) 3% pet. BHA (butylated hydroxyanisole) (icr) 2% pet. + + +; 2% alc. BHT (butylated hydroxytoluene) (icr) 2% pet. + + +; 2% alc. Bifonazole 1% alc. 49_907_928 05.11.2005 12:13 Uhr Seite 911

Patch Test Concentrations and Vehicles Chapter 49 911

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Bioban ® CS-1135 1% pet. + + + Bioban ® CS-1246 1% pet. + + Bioban® P 1487 1% pet. 0.5% pet. 1% pet. Biocheck 60 ® 0.2% aq. Bis(aminopropyl)-lauramine 0.01–0.1–1% aq. Bis(dibutyldithiocarbamato) zinc See Zinc dibutyldithiocarbamate Bis(diethyldithiocarbamato) zinc See Zinc diethyldithiocarbamate BIS-EMA 1% pet. + BIS-GMA 2% pet. + + + BIS-MA 2% pet. + Bismarck Brown (vesuvine brown, 0.5% pet. + + basic brown 1, CI 21000) Bismuth neodecanoate 1% pet. Bisphenol A See 4,4´-isopropylidenediphenol Bisphenol A dimethacrylate 2% pet. Bithionol (ph) (icr) 1% pet. + + + Black rubber mix (N-isopropyl-N-phenyl-p- 0.25% pet. phenylenediamine, N-cyclohexyl-N-phenyl-p- phenylenediamine, N, N-diphenyl-p- phenylenediamine) Boric acid 10% pet. Brilliant black 0.1% coca/glyc. Brilliant lake red R (D&C red 31, CI 15800) 1% pet. (ph) (icr) 5-Bromo-4´-chlorosalicylanilide (ph) 1% pet. 2-Bromo-2-nitropropane-1,3-diol (icr) 0.5% pet. 0.25% pet. 0.5% pet. Budesonide 0.1% pet. + 0.01% pet. + Bucillamine (de) 1% pet. Bufexamac 5% pet. + + Bupivacaine 1% pet. Butacaine 5% pet. 1,4-Butanediol diacrylate (BUDA) 0.1% pet. + 1,4-Butanediol dimethacrylate (BUDMA) 2% pet. + + Butethamine hydrochloride 5% pet. Butyl acrylate (BA) 0.1% pet. + + Butyl aminobenzoate 5% pet. Butylated hydroxyanisole See BHA Butylated hydroxytoluene see BHT 4-tert-Butylbenzoic acid 1% pet. p-tert-Butylcatechol (PTBC) 0.25% pet + 1% pet. Butyl glycidyl ether 0.25% pet. t-Butyl hydroquinone 1% pet. + + + n-Butyl methacrylate (BMA) 2% pet. + Butyl methoxydibenzoylmethane (ph) 10% pet. + + + Butylparaben (icr) 3% pet. + + + p-tert-Butylphenol 1% pet. + + + p-tert-Butylphenolformaldehyde resin (PTBP) 1% pet. + + + Cadmium chloride (ph) 0.5% pet. 1% aq. + Caine mix I (procaine hydrochloride, 3.5% pet dibucaine hydrochloride). Caine mix II (dibucaine hydrochloride, 10% pet. lidocaine, tetracaine) Caine mix III (benzocaine, dibucaine, tetracaine) 10% pet Caine mix IV (amylocaine, lidocaine, prilocaine) 10% pet Calcipotriol 2 µg/ml alc. Camphor (icr) 1% pet. Camphoroquinone 1% pet. Cananga odorata (cananga oil, ylang-ylang oil) 5% pet. 2% pet. Captan 0.5% aq. or pet. 0.5% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 912

912 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Captopril (de) 0.1–3% pet Carbamazepine (de) (fde) (phde) 10% pet. or alc. / 0.01–10% pet. Carba mix (N, N-diphenylguanidine, 3% pet. 3% pet. zinc dibutyl dithiocarbamate, zinc diethyldithiocarbamate) Carbenicillin (de) 5% aq. Carprofen 5% pet. 49 Carteolol 1% aq. Castor oil Pure CD 2 (color developer 2) See Methyl-3-amino-4-N, N- diethyl-aniline CD 3 (color developer 3) See Amino-4-N-ethyl-N-(methane- sulfonaminoethyl)-m-toluidine CD 4 (color developer 4) See 4-(N-Ethyl-N-2-hydroxyethyl)- 2-methylphenylenediamine sulfate Cedarwood oil See Cedrus atlantica Cedrus atlantica (cedarwood oil) (ph) 10% pet. + + Celecoxib (de) 10% pet. Cetalkonium chloride 0.1% pet. 0.1% pet. Cetrimide See Cetrimonium bromide Cetrimonium bromide (Cetrimide) 0.25% pet. Cetyl alcohol (icr) 5% pet. + Cetyl alcohol, stearyl alcohol 20% pet. + + + Cetylpyridinium chloride 0.1% pet. + + Chamomilla recutita (chamomile extract) (icr) 2.5% pet. Chamomilla romana see Anthemis nobilis Chlorambucil 2% pet. Chloramine-T (icr) 0.05% aq. Chloramphenicol (icr) 5% pet. + + 10% pet. Chlorhexidine diacetate (ph, icr) 0.5% aq. Chlorhexidine digluconate (ph) (icr) 0.5% aq. + + + Chlormezanone (fde) 10% pet. or alc. Chloroacetamide 0.2% pet. + + + p-Chloro-m-cresol (PCMX) (icr) 1% pet. + + + 5-Chloro-2-methyl-4-isothiazolin-3-one 0.01–0.02% aq. 5-Chloro-2-methylisothiazol-3-one (MCI), 0.01% aq. + + + 2-methylisothiazol-3-one (MI) 5-Chloro-1-methyl-4-nitroimidazole 0.1% pet. Chlorothalonil (icr) 0.001–0.01% acet. Chloroxylenol 1% pet. 0.5% pet. 1% pet. Chlorphenesin 1% pet. Chlorpheniramine maleate 5% pet. + Chlorpromazine hydrochloride (phde) (icr) 0.1–1% pet. or aq. 0.1% pet. 1% pet. Chlorquinaldol (ph) 5% pet. + + + Chlortetracycline hydrochloride 5% pet. 1% pet. Chromic chloride 1% pet. Chromic potassium sulfate 2% aq. Chromic sulfate 0.5% pet. Chrysanthemum cinerariaefolium 1% pet. + (pyrethrum) (icr) Chrysanthemum parthenium 1% pet. (feverfew flower extract) Cimetidine (de) 1% aq. Cinchocaine® See Dibucaine hydrochloride Cinnamal (cinnamic aldehyde) (ph) (icr) 1% pet. + 2% pet. + Cinnamic acid (icr) 5% pet. Cinnamomum cassia (cinnamon oil) (ph)(icr) 0.5% pet. Cinnamyl alcohol 1–5% pet. 1% pet. 2% pet. 1% pet. Cinnoxicam 1% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 913

Patch Test Concentrations and Vehicles Chapter 49 913

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Ciprofloxacin (fde) 10% pet. Citiolone (fde) 10% DMSO Citronellal 2% pet. Citrus aurantium dulcis (neroli oil) (ph) 2% pet. + + Citrus bergamia (bergamot oil) (ph) 2% pet. Citrus dulcis (orange oil) (ph) 2% pet. Citrus limonum (lemon oil) (ph) 2% pet. + + Clioquinol® (icr) 5% pet. + + + Clarithromycin (fde) 10% aq. Clindamycin phosphate (de) 1–20% aq. or pet. Clobetasol-17-propionate (icr) 1% alc. 0.25% pet. 1% pet. 0.25% pet. Clobetasone butyrate 1% alc. Clomipramine (phde) 0.1% pet. Clonidine 1% pet. Cloprednol 1% alc. Clotrimazole 5% pet./1% alc. + (pet.) + (pet) Clove oil See Eugenia caryophyllus Cloxacillin (de) (icr) Pure Coal tar See Pix ex carbone Cobalt chloride (ph) (icr) 1% pet. + + + Cobaltous sulfate (ph) 2.5% pet. Cocamide DEA 0.5% pet. + + + Cocamidopropyl betaine 1% aq. + + 1% alc./aq. Codeine phosphate (de) 0.05% aq. Cold cream Pure Colophonium (rosin) (ph) (icr) 20% pet. + + + Compositae mix (Tanacetum vulgare, 6% pet. 5% pet. 5% pet. Arnica montana, parthenolide, Chamomilla romana, Achillea millefolium) Congo red 2% pet. Copper oxide See Cupric oxide Copper 8-quinolinate 1% pet. Copper sulfate See Cupric sulfate Corticosteroid mix (budesonide, tixocortol 2.1% pet. pivalate, hydrocortisone-17-butyrate) Coumarin (ph) 1% pet. Coumarone indene resin 20% pet. p-Cresol 1% aq. Cresyl glycidyl ether (icr) 0.25% pet. + + Croconazole 1% alc. Cromoglicate sodium See Cromolyn Cromolyn (cromoglicate sodium) (icr) 2% aq. 2% pet. Crotamiton 3% pet. Cupric oxide (copper oxide) 5% pet. Cupric sulfate (copper sulfate) (icr) 1% pet. 2% pet. 1% aq.; 2% pet. Cyclohexanone resin 1% pet. Cycloheximide 1% pet. N-Cyclohexylbenzothiazyl sulfenamide (CBS) 1% pet. + + + N-Cyclohexyl-N-phenyl-p-phenylene-diamine 1% pet. (CPPD) Cyclohexyl thiophthalimide 1% pet. 0.5% pet. + + Cyclomethycaine hydrochloride 1% pet. Cyclopentolate hydrochloride 0.5% aq. Cymbopogon schoenanthus (lemon grass oil) (ph) 2% pet. + + Dandelion See Taraxacum officinale Dazomet 0.1% pet. D and C red 31 See Brilliant lake red R D and C yellow 11 See Solvent yellow 33 49_907_928 05.11.2005 12:13 Uhr Seite 914

914 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

DDT 1% pet. or acet. Desketoprofen 1% pet. Desonide 1% alc. Desoximetasone (ph) 1% alc. Dexamethasone 0.5% pet. Dexamethasone acetate 1% alc. Dexamethasone phosphate 1% alc. 49 Dexamethasone 21-phosphate disodium salt 1% pet. Dexpanthenol See Panthenol Diallyl disulfide 1% -5% pet. 1% pet. 4,4´-Diaminodiphenylmethane (ph) 0.5% pet. + + + Diazolidinyl urea 2% pet. + + + Dibenzothiazyl disulfide (MBTS) 1% pet. + + + Dibenzthione (sulbentine) 3% pet. 1,2-Dibromo-2,4-dicyanobutane See Methyldibromo glutaronitrile Dibromosalicylanilide (ph) 1% pet. Dibucaine hydrochloride (Cinchocaine ®) (ph) 5% pet. + + + Dibutyl phthalate 5% pet. + + + Dibutylthiourea 1% pet. + + + Dibutyl- and diethylthiourea mix 1% pet. Dichlorophene (ph) 0.5% pet. + 1% pet. + Diclofenac sodium 1% pet. 5% pet. Dicyanodiamide 0.1% aq. Dicyclohexylcarbodiimide 0.1% acet. and lower Diethanolamine 2% pet. + + Diethyleneglycol diacrylate (DEGDA) 0.1% pet. + Diethylenetriamine (DETA) 1% pet. 0.5% pet. + Di-2-ethylhexyl phthalate (DEHP) See Dioctyl phthalate Diethyl phthalate 5% pet. Diethylstilbestrol 1% pet. Diethylthiourea 1% pet. + Diflorasone diacetate 1% alc. Diflucortolone pivalate 1% alc. Difolatan 0.1% pet. Diglycolamine [2-(2-aminoethoxy)ethanol] 1% pet. Dihydroquinidine (de) Pulverized tablet, pure Dihydrostreptomycin 0.1% pet. 4,4´-Dihydroxydiphenyl 0.1% pet. 0.1% pet. Diisopropyl carbodiimide 0.1% acet. and lower Diltiazem (de) 1% pet. Dimethylaminoethyl ether 1% pet. N, N-Dimethylaminoethyl methacrylate 0.2% pet. Dimethylaminopropylamine 1% aq. Dimethylaminopropyl ethyl carbodiimide 0.1% acet. and lower Dimethyl dihydroxyethyleneurea 4.5% aq. Dimethylol dihydroxyethyleneurea 5% aq. 4,4-Dimethyloxazolidine/3,4,4,trimethyl- See Bioban® CS-1135 oxazolidine N, N´-Dimethyl-p-phenylenediamine 0.25% pet. Dimethyl phthalate 5% pet. + + Dimethylol propylene urea 5% aq. N, N-Dimethyl-p-toluidine 2% pet. 5% pet. 2% pet. N, N-di-β-Naphthyl-p-phenylenediamine 1% pet. (DBNPD) 2,4-Dinitrochlorobenzene (DNCB) (icr) 0.01–0.1% aq. or acet. Dioctyl phthalate (di-2-ethylhexyl phthalate) (icr) 5% pet. + 2% pet. + Dipentamethylenethiuram disulfide 0.25% pet. 1% pet. 0.25% pet. Dipentamethylenethiuram tetrasulfide 0.25% pet. Dipentene (D-limonene) 2% pet. + 1% pet. + Diperocaine 1% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 915

Patch Test Concentrations and Vehicles Chapter 49 915

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Diphenhydramine hydrochloride (ph) 1% pet. + 1,3-Diphenylguanidine (DPG) (icr) 1% pet. + + + Diphenylmethane 4,4-diisocyanate (MDI) (icr) 2% pet. + 1% pet. N, N’-Diphenyl-p-phenylenediamine (DPPD) 1% pet. 0.25% pet. 1% pet. 0.25% pet. Diphenylthiourea (DPTU) 1% pet. + + + Dipivalyl epinephrine hydrochloride 1% aq. Dipyrone (metamizole) (fde) (icr) 10% pet. 1% pet. Direct black 38 (CI 30235) 1% pet. Direct orange 34 (CI 40215) 1% pet. 5% pet. Disodium EDTA (edetic acid disodium salt) 1% pet. + + + Disperse black 1 (CI 11365) 1% pet. Disperse black 2 (CI 11255) 1% pet. Disperse blue 1 (CI 64500) 1% pet. + Disperse blue 3 (CI 61505) 1% pet. + + + Disperse blue 7 (CI 62500) 1% pet. Disperse blue 26 (CI 63305) 1% pet. Disperse blue 35 (ph) 1% pet. + Disperse blue 85 (CI 11370) 1% pet. + Disperse blue 102 1% pet. Disperse blue 106 (CI 111935) 1% pet. + Disperse blue 124 1% pet. + Disperse blue 153 1% pet. + Disperse blue mix (124/106) 1% pet. + + Disperse brown 1 (CI 11153) 1% pet. + Disperse orange 1 (CI 11080) 1% pet. + Disperse orange 3 (CI 11005) 1% pet. + + + Disperse orange 13 (CI 26080) 1% pet. Disperse orange 76 1% pet. Disperse red 1 (CI 11110) 1% pet. + + + Disperse red 11 (CI 62015) 1% pet. + + Disperse red 17 (CI 11210) 1% pet. + + + Disperse red 153 1% pet. Disperse yellow 1 (CI 10345) 1% pet. Disperse yellow 3 (CI 11855) 1% pet. + + + Disperse yellow 9 (CI 10375) 1% pet. + + + Disperse yellow 27 1% pet. Disperse yellow 39 1% pet. Disperse yellow 49 1% pet. Disperse yellow 54 (CI 47020) 1% pet. Disperse yellow 64 (CI 47023) 1% pet. 4,4´-Dithiodimorpholine 1% pet. Dithranol 0.02% pet. Di-o-tolyl biguanidine 1% pet. Diurethane dimethacrylate 2% pet. 2% pet. DMDM hydantoin 2% aq. + + + Dodecyldi(aminoethyl)glycine (DDAG) 0.5% aq. Dodecyl dimethyl ammonium chloride 0.01–0.1–1% aq (quaternium-12). Dodecyl gallate (lauryl gallate) 0.3% pet. 0.25% pet. 0.3% pet. Dodecyl mercaptan 0.1% pet. + + Doxepin 5% pet. Doxycycline (phde) (fde) 10% alc. or pet. Drometrizole [2-(2´-hydroxy-5´-methyl- 1% pet. phenyl)benzotriazole] Echothiophate iodine 1% aq. Econazole nitrate 1% alc. + Edetic acid disodium salt See Disodium EDTA Enilconazole 1% alc. Eosine (ph) 5% pet. 50% pet. Ephedrine hydrochloride (de) 5% aq. 1% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 916

916 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Epichlorohydrin 0.1% pet. Epinephrine 1% aq. Epoxy acrylate 0.5% pet. Epoxy resin (icr) 1% pet. + + + Epoxy resin, cycloaliphatic 0.5% pet. Erythromycin (base and salts) (de) 1% pet. + + Erythrosine (ph) 0.25% pet.; 49 alc./glyc. Estradiol 2% alc. Ethacridine lactate monohydrate (Rivanol®) 2% pet. Ethanolamine (monoethanolamine) 2% pet. 2% pet. Ethenzamide (fde) 20% pet. Ethoxyquin (ph) 1% pet. 0.5% pet. Ethyl acrylate (EA) 0.1% pet. + Ethyl alcohol 10% aq. 7-Ethylbicyclooxazolidine See Bioban® CS-1246 Ethylbutyl thiourea 1% pet. Ethyl cyanoacrylate 5% pet. 10% pet. Ethylenediamine dihydrochloride (ph) (icr) 1% pet. + + + Ethyleneglycol dimethacrylate (EGDMA) 2% pet. + + + Ethylene thiourea See 2-Imidazolidinethione Ethylene urea See 2-Imidazolidinone Ethyleneurea + melamine-formaldehyde 5% pet. 2-Ethylhexyl acrylate (EHA) 0.1% pet. + + 2-Ethylhexyl-p-dimethylaminobenzoate See Octyl dimethyl PABA 2-Ethylhexyl-p-methoxycinnamate see Octyl methoxycinnamate 4-(N-Ethyl-N-2-hydroxyethyl)-2-methyl- 1% pet. + + phenylenediamine sulfate (CD 4) Ethyl methacrylate (EMA) 2% pet. + Ethylparaben (icr) 3% pet. + + + Ethyl sebacate 2% alc. N-Ethyl-4-toluenesulfonamide 0.1% pet. Etofenamate (icr) 2% pet. Eucalyptus globulus (eucalyptus oil) 2% pet. + + Eucerin, anhydrous (lanolin) Pure + Eugenia caryophyllus (clove oil) 2% pet. + + Eugenol (icr) (ph) 2% pet. 1% pet. + 1% pet. Euxyl ® K 400 1% pet. 1% pet. 1% pet. Evernia prunastri (oak moss absolute) (ph) 1% pet. + + + Evernic acid (ph) 0.1% pet. Fagus sylvatica (beech tar) 3% pet. Famciclovir 10% aq. Feneticillin (de) Pure Fenoprofen 5% pet. Fentichlor (ph) 1% pet. Fenticonazole 1% alc. Feprazone 5% pet. Ferrous chloride 2% alc./ glyc. Ferrous sulfate 5% pet. Feverfew flower extract See Chrysanthemum parthenium Fluazinam 0.5% pet. Fludrocortisone acetate 1% alc. Flufenamic acid 1% pet. Flumethasone acetate 1% alc. Fluocinolone acetonide 1% alc. Fluocinonide 1% alc. Fluocortolone 1% alc. Fluorouracil 1% pet. Flurbiprofen 5% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 917

Patch Test Concentrations and Vehicles Chapter 49 917

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Flutamide (phde) 1–20% acet. or pet. Fluticasone propionate 1% alc. Folpet 0.1% pet. Formaldehyde (ph) (icr) 1% aq. + + + Formic acid 1% aq. Fragrance mix (ph) (cinnamic alcohol, 8% pet. + + + cinnamal, hydroxy citronellal, α-amyl-cinnamal, geraniol, eugenol, iso-eugenol, oakmoss absolute) Framycetin (neomycin B) 20% pet. 10% pet. Fusidic acid sodium salt 2% pet. + + Gallium oxide 1% pet. Ganciclovir 20% aq. Gentamicin sulfate (icr) 20% pet. + + + Geraniol (icr) 2% pet. 1% pet. 1% pet. 1% pet. Geranium oil, Bourbon 2% pet. Glutaral 0.2–0.3% pet. 0.3% pet. 0.2% pet. 0.3% pet. Glyceryl thioglycolate (icr) 1% pet. + + + Glyoxal 1% pet./aq. + (pet) + (pet) Glyphosate 1–10% aq. Gold sodium thiomalate (de) (icr) 5% pet. Gold sodium thiosulfate (icr) 0.25% pet. 2% pet. Griseofulvin (phde) 1% pet. Grotan® BK See 1,3,5-Tris(2-hydroxyethyl)- hexahydrotriazine Halcinonide 1% alc. Haloprogin 1% pet. Halomethasone 1% alc. Hexachlorophene (ph) 1% pet. + + +; 0.5% pet. Hexamethylene diisocyanate (HDI) 0.1% pet. + Hexamethylenetetramine See Methenamine Hydroxymethylpentylcyclohexene- 5% pet. carboxaldehyde (Lyral®) 1,6-Hexanediol diacrylate (HDDA) 0.1% pet. + Hexylresorcinol 0.25% pet. + + Homosalate (homomenthyl salicylate) 5% pet. Homatropine 1% aq. Hydrangenol 0.1% pet. Hydrazine sulfate 1% pet. + + Hydroabietyl alcohol 10% pet + + Hydrochlorothiazide (phde) 1–10% pet. Hydrocortisone (ph) 1% alc./pet. + (pet.) + (pet.) Hydrocortisone aceponate 1% alc. Hydrocortisone acetate 1% alc. Hydrocortisone buteprate 1% alc. Hydrocortisone-17-butyrate 1% alc. 0.1% pet. + 0.1% pet. Hydrogen peroxide 3% aq. Hydromorphone (de) 2% aq. Hydroquinone (HQ) 1% pet. + + + Hydroquinone monobenzylether See Monobenzone Hydroxycitronellal (ph) 1–5% pet. 1% pet. 1% pet. 1% pet. 2-Hydroxyethyl acrylate (2-HEA) 0.1% pet + + 2-Hydroxyethyl methacrylate (HEMA) 2% pet. 1% pet. + 1% pet. Hydroxyisohexyl-3-cyclohexenecarboxaldehyde See Hydroxymethylpentylcyclo- hexenecarboxaldehyde Hydroxylammonium chloride 0.1% aq. Hydroxylammonium sulfate 0.1% aq. 2-Hydroxymethyl-2-nitro-1,3-propanediol See Tris(hydroxymethyl)nitromethane 49_907_928 05.11.2005 12:13 Uhr Seite 918

918 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

2-(2´-Hydroxy-5´-methylphenyl)benzotriazole See Drometrizole Hydroxymethylpentylcyclohexene- 5% pet. 5% pet. carboxaldehyde (Lyral®) 2-Hydroxypropyl acrylate (HPA) 0.1% pet. + 2-Hydroxypropyl methacrylate (HPMA) 2% pet. + + 2-Hydroxy-4-trifluoromethylbenzoic acid See Triflusal Hypericum perforatum (hypericum oil) 0.5% pet. 49 Ibuprofen (fde) 5–10% pet. Ibuproxam (ph) 2.5% pet. Ichthammol (ammonium bituminosulfonate) 10% pet. + Idoxuridine 1% pet. + 2-Imidazolidinethione (ethylene thiourea) 1% pet. 2-Imidazolidinone (ethylene urea) 1% pet. Imidazolidinyl urea 2% pet. + + + Indium (III) chloride 1% pet. Indometacin 5% pet. 1% pet. Iodoform 5% pet. Iodopropynyl butylcarbamate 0.2% pet. 0.1% pet. Isoamyl-p-methoxycinnamate (ph) 10% pet. + + Isoconazole nitrate 1% alc. Iso-eugenol 2% pet. 1% pet. + 1% pet. Isophoronediamine (IPD) 0.1% pet. 0.5% pet. + 0.5% pet. Isophorone diisocyanate (IPDI) 1% pet. + Isopropyl dibenzoylmethane (ph) 2% pet. 4,4´-Isopropylidenediphenol (Bisphenol A) 1% pet. + + Isopropyl myristate 10% pet. 20% pet. 10% pet. N-Isopropyl-N´-phenyl-p-phenylenediamine 0.1% pet. + + + (IPPD) Jasminum officinale (jasmine absolute, synthetic) 5–10% pet. 2% pet. Juniperus (juniper tar) 3% pet. Kanamycin sulfate 10% pet. + + + Ketoconazole (icr) 1% alc. Ketoprofen (phde) (icr) 1% pet. Ketotifen 2.5% pet./0.7% aq. Lanette E ® 20% pet. Lanette N ® 20% pet. Lanoconazole 1% alc. Lanolin Pure 30% pet. Lanolin alcohol (wool wax alcohols) (icr) 30% pet. + + + Lanolin alcohol and paraffinum liquidum 50% pet. + + + (Amerchol® L 101) Laurus nobilis (laurel oil) 2% pet. + + Lauryl gallate See Dodecyl gallate Lavandula angustifolia (lavender, absolute) (ph) 2% pet. Lemon grass oil See Cymbopogon schoenanthus Lemon oil See Citrus limonum Levobunolol hydrochloride 1% aq. Lichen acid mix (atranorin, evernic acid, 0.3% pet. usnic acid) Lidocaine hydrochloride (icr) 5% pet. 15% pet. + 15% pet. D-Limonene See Dipentene Lincomycin hydrochloride 1% aq. Lindane (icr) 1% pet. Lomefloxacin (phde) 1–10% pet. Lyral® See Hydroxymethylpentylcyclo- hexenecarboxaldehyde Mafenide 10% pet. + Malathion 0.5% pet. Maneb (ph) 0.5–1% pet. Mechlorethamine hydrochloride (icr) 0.02% aq. 49_907_928 05.11.2005 12:13 Uhr Seite 919

Patch Test Concentrations and Vehicles Chapter 49 919

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Medroxyprogesterone acetate 1% pet. Mefenamic acid (fde) 1–10% pet. Melaleuca alternifolia (tea tree oil) 5% pet. Melamine formaldehyde 7% pet. Mentha piperita (peppermint oil) (icr) 2% pet. + + Menthol (icr) 1% pet. 2% pet. 1% pet. Mepivacaine 1% pet. + Merbromin (mercurochrome) 2% aq. or pet. 2-Mercaptobenzimidazole See 2-Benzimidazolethiol Mercaptobenzothiazole (MBT) (icr) 2% pet. + + + Mercapto mix (mercaptobenzothiazole 1% pet. + + + dibenzothiazyl disulfide, morpholinyl mercaptobenzothiazole, N-cyclohexyl-benzo- thiazyl sulfenamide) Mercuric chloride 0.1% pet. + Mercurochrome See Merbromin Mercury (icr) 0.5% pet. + + Mesulfen 5% pet. Metamizol See Dipyrone Metanil yellow See Acid yellow 36 Methenamine (hexamethylenetetramine) 2% pet 1% pet. + 1% pet. Methyl-3-amino-4-N, N-diethyl-aniline (CD 2) 1% pet. 1% pet. p-Methylaminophenol sulfate (Metol®)(icr) 1% pet. 1% pet. Methyl anthranilate 5% pet. 4-Methylbenzylidene camphor (ph) 10% pet. + + + Methylcoumarin (6-MC) (ph) 1% pet. Methyldibromo glutaronitrile (1,2-dibromo-2,4- 0.3% pet. + + 0.1% pet. dicyanobutane) Methyldichlorobenzene sulfonate 0.1% pet. N, N-Methylenebisacrylamide (MBAA) 1% pet. + Methylene-bis(methyloxazolidine) 1% pet. α-Methylene-γ-butyrolactone (tulipalin) 0.001% alc. 0.01% pet. Methylhydroquinone (MHQ) 1% pet. 2-Methyl-4-isothiazolin-3-one 0.01–0.02% aq. Methyl methacrylate (MMA) 2% pet. + + + N-Methylolchloroacetamide 0.1% pet. Methyl orange 2% pet. Methylparaben (icr) 3% pet. + + + Methylprednisolone aceponate 1% alc. Methylprednisolone acetate 1% alc. Methyl salicylate (icr) 2% pet. + 3-Methyl thiazolidone 2-thion 1% pet. Methyl-p-toluene sulfonate 0.1% pet. Methyl violet 0.5% pet. Metipranolol 2% aq. Metol ® See p-Methylaminophenol sulfate Metoprolol (de) 3% aq. / 10% pet. Metronidazole (fde) 1% pet., 50% pet. 1% pet. Mexenone See Benzophenone-10 Miconazole nitrate 1% alc. + Minoxidil 5% in aq. + 20% prop. glyc. Mitomycin C 0.1% pet. Mofebutazone 1% pet. Mometasone furoate 1% alc. Monobenzone (monobenzylether of 1% pet. + + + hydroquinone) Monobenzylether of hydroquinone See Monobenzone Monoethanolamine See Ethanolamine 49_907_928 05.11.2005 12:13 Uhr Seite 920

920 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

2-Monomethylol phenol 1% pet. Morpholinyl mercaptobenzothiazole (MOR) (icr) 0.5% pet. + 1% pet. 1% pet. Mupirocin 10% pet. Musk ambrette (ph) 5% pet. + Musk ketone (ph) 1% pet. Musk mix (xylene, tibetene, moskene, ketone) 3% pet. Musk moskene (ph) 1% pet. 49 Musk xylene (ph) 1% pet. Myristyl alcohol 5% pet. Myroxylon pereirae (balsam of Peru) (ph) (icr) 25% pet. + + + Myroxylon toluiferum (balsam of Tolu) 20% pet. 10% alc. 20% pet. Naftifine hydrochloride 5% alc. 1-Naphthalenethiourea (ANTU) 1% pet. Naphthol AS (CI 37505) 1% pet. + + Naphthyl mix (N, N-di-β-naphthyl-p-phenylene- 1% pet. diamine, N-phenyl-2-naphthy-lamine) Naproxen 5% pet. Narcissus absolute 2% pet. Neomycin sulfate (icr) 20% pet. + + + Neroli oil See Citrus aurantium dulcis Neticonazole 1% alc. Nickel sulfate (icr) 5% pet. + + + Nicotine (icr) 10% pet. Nifuroxazide (de) 0.001–1% aq./10% pet. Nigrosine 1% pet. + Nimesulide (fde) 1–10% pet. (Nitrobutyl)morpholine/(ethylnitrotrimethylen) see Bioban® dimorpholine Nitrofurazone 1% pet. + + Nitroglycerin (icr) 1% pet. 2-Nitro-p-phenylenediamine (ONPPD) 1% pet. + + Nonoxynol 5% aq. Nordihydroguaiaretic acid 2% pet. Norethisterone acetate 1% alc. Nystatin (de) 2% pet. + + Oak moss absolute See Evernia prunastri Octyl dimethyl PABA (2-ethylhexyl-p-dimethyl- 10% pet. + + + aminobenzoate) (ph) Octyl gallate 0.3% pet. 0.25% pet. 0.3% pet. Octylisothiazolinone 0.1% pet. 0.025% pet. + Octyl methoxycinnamate (2-ethylhexyl-p- 10% pet. + + + methoxycinnamate) (ph) Octyl phthalate 5% pet. Octyl triazone (ph) 10% pet. Ofloxacin (fde) 20% pet. Olaquindox (ph) 1% pet. Olea europaea (olive oil) Pure Oleamidopropyl dimethylamine 0.1% aq. Oleyl alcohol 30% pet. Oligotriacrylate 480 0.1% pet. + Olive oil See Olea europaea Orange oil See Citrus dulcis Orthocaine 1% pet. Oxiconazole 1% alc. Oxprenolol (de) 10% pet. Oxybenzone See Benzophenone-3 Oxybuprocaine (benoxinate) 1% pet. Oxyphenbutazone (de) (icr) 1–5% pet. 10% pet. Oxytetracycline 3% pet. + + PABA (ph) 10% pet. + + + 49_907_928 05.11.2005 12:13 Uhr Seite 921

Patch Test Concentrations and Vehicles Chapter 49 921

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Palladium chloride (icr) 1% pet. + 2% pet. + Panthenol (dexpanthenol) (icr) 50% aq. 5% pet. 5% pet. Papain 1% pet. 1% pet. Paraben mix (butyl, ethyl, methyl, propyl- 16% pet. 16% pet. 16% pet paraben) (icr). Paracetamol 10% pet. Paraquat 0.1% pet. Paromomycin 10% pet. Parthenolide 0.1% pet. Pecilocin 1% pet. PEG 6 (and) PEG 32 (polyethylene glycol Pure + + ointment) PEG 400 (icr) Pure Penbutolol sulfate 2% aq. Penethamate 1% pet. D-Penicillamine 1% aq. Penicillin G (de) (icr) 100,000 U/g aq. or pet. Pentachloronitrobenzene 1% pet. Pentaerythritol triacrylate (PETA) 0.1% pet. + Peppermint oil See Mentha piperita Perfume mix (cinnamic alcohol, cinnamal, 6% pet. hydroxycitronellal, eugenol, iso-eugenol, geraniol) Petrolatum, white (icr) Pure + + + Phenacetin 10% pet. Phenazone See Antipyrine Phenidone ® See 1-Phenyl-3-pyrazolidinone Phenobarbital (de) 1–20% pet. Phenol-formaldehyde resin (P-F-R-2) 5% pet. 1% pet. 5% pet. Phenolphthalein 0.5% pet. Phenoxyethanol (icr) 1% pet. + + + Phenyl-azo-2-naphthol (PAN) 0.1% pet. Phenylbenzimidazole sulfonic acid (ph) 10% pet. + + + Phenylbutazone (de) (icr) 1–5% pet. 10% pet. p-Phenylenediamine dihydrochloride 0.5% pet. p-Phenylenediamine free base (ph) (icr) 1% pet. + + + Phenylephrine hydrochloride 10% aq. + 10% coca Phenyl glycidyl ether (icr) 0.25% pet. 0.25% pet Phenylhydrazine 1% pet. α-Phenylindole 2% pet. Phenyl isocyanate 0.1% pet. Phenylmercuric acetate (icr) 0.01% aq. 0.05% pet. + 0.05% pet. Phenylmercuric borate 0.05% pet. Phenylmercuric nitrate (icr) 0.05% pet. 0.01% pet. Phenyl-β-naphthylamine (PBN) 1% pet. + + o-Phenylphenol (ph) (icr) 1% pet. Phenyl-p-phenylenediamine 0.25% pet. 1-Phenyl-3-pyrazolidinone (Phenidone®) 1% pet. + + Phenyl salicylate 1% pet. + + + Phosphorus sesquisulfide (icr) 0.5% pet. Picric acid 1–2% pet. Piketoprofen 2.5% pet. Pilocarpine hydrochloride (icr) 1% pet. 1% aq. 1% alc./ glyc. Pindolol 2% pet. Pinus (pine tar) 3% pet. + + Piperazine (de) 1% pet. or aq. Piroxicam (phde) (de) 1–10% pet. Pix ex carbone (coal tar) (ph) (icr) 5% pet. + + Polidocanol 3% pet. + + 49_907_928 05.11.2005 12:13 Uhr Seite 922

922 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Polyethylene glycol-400 See PEG 400 Polyethylene glycol ointment See PEG 6 (and) PEG 32 Polymyxin B sulfate 3% pet. + 5% pet. + Polyoxyethylene sorbitan monolaurate See Polysorbate 20 Polyoxyethylene sorbitan monooleate See Polysorbate 80 Polyoxyethylene sorbitan monopalmitate See Polysorbate 40 Polysorbate 20 (polyoxyethylene sorbitan 5% pet. 49 monolaurate) (icr) Polysorbate 40 (polyoxyethylene sorbitan 5% pet. 10% pet. monopalmitate) (icr) Polysorbate 80 (polyoxyethylene sorbitan 5% pet. + 10% pet. monooleate) (icr) Potassium dichromate (ph) 0.5% pet. + & 0.25% + + pet. Potassium dicyanoaurate 0.002% aq. 0.1% aq. 0.002% pet. Povidone-iodine (icr) 10% aq. or pet. 10% aq. PPP-HB 5% alc. Pramocaine hydrochloride 1% pet. Pravastatin (de) Pulverized tablet pure Prednicarbate 1% alc. Prednisolone 1% alc. 1% pet. 0.5% pet. Prilocaine hydrochloride 5% pet. + Primin 0.01% pet. + + + Pristinamycin (icr) (de) 1–10% pet. or aq. Procaine hydrochloride (ph) (icr) 1–2% pet. 2% pet. 1% pet. 2% pet. Proflavine hydrochloride 1% pet. Promethazine hydrochloride (phde) (icr) (fde) 1–10% pet. 0.1% pet. 1% pet. 2% pet. Propanidid 5% pet. Propantheline bromide 5% pet. Proparacaine (proxymetacaine) 2% pet. Propicillin (de) 20% pet. Propionic acid 3% pet. Propipocaine 1% pet. Propolis 10% pet. + + + Propranolol hydrochloride (de) 10–20% pet. 2% pet. Propylene glycol (icr) 5% pet. or aq. 5% pet./ 5% pet. 20% aq. 20% aq. Propylene oxide 0.1–1% alc. Propyl gallate 1% pet. 0.5% pet. 1% pet. 0.5% pet. Propylparaben (icr) 3% pet. + + + Propyphenazone (icr) 1% pet. Proxymetacaine See Proparacaine Pyrazinamide (de) 1–10% alc. Pyrethrum See Chrysanthemum cinerariaefolium Pyridoxine hydrochloride (ph) (de) 10% pet. Pyrilamine maleate 2% pet. Pyritinol (phde) 20% pet. Pyrogallol 1% pet. + + Pyrrolnitrin 1% pet. Quaternium-12 See Dodecyl dimethyl ammonium chloride Quaternium-15 1% pet. + + + Quindoxin (ph) 0.1% pet. Quinidine sulfate (phde) 1% pet. + + Quinine sulfate (phde) 1% pet. + 25% pet. Quinoline mix (clioquinol, chlorquinaldol) 6% pet. 4-Quinolines (phde) 10% pet. Quinoline yellow 0.1% coca 49_907_928 05.11.2005 12:13 Uhr Seite 923

Patch Test Concentrations and Vehicles Chapter 49 923

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Ranitidine (de) 1% pet. Reactive black 5 (CI 20505) 1% pet. Reactive blue 21 (CI 18097) 1% pet. Reactive blue 238 1% pet. Reactive orange 107 1% pet. Reactive red 123 1% pet. Reactive red 228 1% pet. Reactive red 238 1% pet. Reactive violet 5 (CI 18097) 1% pet. Resorcinol (icr) 1% pet. + + Resorcinol monobenzoate 1% pet. Resorcinol/formaldehyde resin 5% pet. Retinoic acid 0.005% alc. Rhodium sulfate (icr) 0.05% aq. Ribostamycin 20% pet. Rifamycin (icr) 2.5% pet. Rivanol® See Ethacridine lactate monohydrate Rosa (rose oil) 0.5% pet. Rosa centifolia (rose oil, Bulgarian) 2% pet. Rosemary (rosemary oil) 0.5% pet. Rosin See Colophonium Rubidium iodide 1% pet. Ruthenium (icr) 0.1% pet. Salicylaldehyde 2% pet. + + Salicylic acid (icr) 1% pet. 5% pet. Sandalwood oil See Santalum album Santalum album (sandalwood oil) (ph) 10% pet. 2% pet. Scopolamine hydrobromide 0.25% aq. Sertaconazole 1% alc. Sertraline (de) 5–10% alc. or pet. Sesamum indicum (sesame oil) Pure Sesquiterpene lactone mix (alantolactone, 0.1% pet. + + costunolide, dehydrocostus lactone) Silicon tetrachloride 2% pet. Silver colloidal 0.1% pet. Silver nitrate (ph) 1% aq. Silver protein 3% pet. Simvastatin (phde) 10% pet. Sisomicin 20% pet. Sodium benzoate (icr) 5% pet. + + + Sodium disulfite 1% pet. Sodium fusidate See Fusidic acid sodium salt Sodium hypochlorite (icr) 0.5% aq. Sodium hyposulfite 1% aq. Sodium lauryl sulfate 0.1% aq. Sodium metabisulfite 1% pet. Sodium nitrite 2% aq./ glyc. Sodium omadine See Sodium pyrithione Sodium pyrithione (sodium omadine) 0.1% aq. Sodium thiosulfoaurate 0.25% pet. 0.25% pet. Sodium valproate (de) 1–5% pet. Solvent red 23 (Sudan III) 2% pet. 1% pet. Solvent red 24 (Sudan IV) 2% pet. Solvent yellow 1 See p-Aminoazobenzene Solvent yellow 33 (D and C yellow 11, CI 47000) 0.1% pet. Sorbic acid (icr) 2% pet. + + +; 2% alc./glyc. Sorbitan laurate (Span® 20) (icr) 5% aq. 49_907_928 05.11.2005 12:13 Uhr Seite 924

924 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Sorbitan oleate (Span® 80) 5% aq. 5% pet. Sorbitan palmitate (Span® 40) 5% aq. Sorbitan sesquioleate (icr) 20% pet. + 2% pet. + Spiramycin sulfate (de) 5–10% pet. Stannous chloride 1% pet. 0.5% pet. 0.5% pet. Stearyl alcohol (icr) 30% pet. + Stepronin (de) 18% sol. 49 Streptomycin (sulfate) (icr) 1–2.5% pet. 5% pet. Styrax benzoin (benzoin resin) (icr) 2% pet. 10% alc./ glyc./aq. Styrenated phenol 1% pet. Sudan III See Solvent red 23 Sudan IV See Solvent red 24 Sulbentine See Dibenzthione Sulconazole nitrate 1% alc. Sulfacetamide 5% pet. Sulfamethoxazole (de) Pure Sulfanilamide (ph) 5% pet. + + + Sulfasalazine (fde) 10% pet. Sulfur dioxide (icr) 2% aq. Sulfur, pharmaceutical (precipitated) 10% pet. Sulindac 1% pet. Sulisobenzone See Benzophenone-4 Suprofen 0.1% pet. Tacalcitol 2 µg/ml alc. Tacrolimus 2.5% pet. Tanacetum vulgare (tansy extract) 1% pet. + + Tansy extract See Tanacetum vulgare Tantalum 1% pet. Taraxacum officinale (dandelion) 2.5% pet. Tea tree oil See Melaleuca alternifolia Tego® 103 G 0.1% aq. Tenoxicam (fde) 1–10% pet. Terpene phenolic resin 20% pet. Testosterone propionate 1% alc. Tetracaine hydrochloride (amethocaine) 1% pet. + 5% pet. + Tetrachlorosalicylanilide (TSCA) (ph) 0.1% pet. Tetracycline hydrochloride 2% pet. 2% pet. Tetraethylene glycol dimethacrylate 2% pet. Tetraethylthiuram disulfide (TETD) 0.25% pet. 1% pet. 0.25% pet. Tetrahydrofurfuryl methacrylate (THFMA) 2% pet. + Tetramethylbenzidine 0.1% pet. Tetramethyl butanediamine 1% pet. Tetramethylol acetylenediurea 5% aq. Tetramethylthiuram disulfide (TMTD) 0.25% pet. 1% pet. 0.25% pet. Tetramethylthiuram monosulfide (TMTM) 0.25% pet. 1% pet. 0.25% pet. Tetrazepam (phde) 10% pet. Thiabendazole 1% pet. Thiamphenicol 5% pet. Thimerosal (icr) 0.1% pet. + + Thioridazine (phde) 1% pet. Thiourea (ph) 0.1% pet. + + + Thioxolone 0.5% alc. Thiram 1% pet. Thiuram mix (tetramethylthiuram monosulfide, 1% pet. + + + tetramethylthiuram disulfide, tetraethylthiuram disulfide, dipentamethylenethiuram disulfide) Tiaprofenic acid (phde) 1% pet. Timolol 0.5% aq. 49_907_928 05.11.2005 12:13 Uhr Seite 925

Patch Test Concentrations and Vehicles Chapter 49 925

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Tin (icr) 50% pet. Tinopal CH 3566 1% pet. Tiocolchicoside 1% pet. Tioconazole 1% alc. Tiopronin (de) 0.3–5% pet. Titanium-(IV)-oxide 0.1% pet. Tixocortol pivalate 0.1–1% pet. 0.1% pet. Tobramycin (de) 5% aq. or 20% pet. α-Tocopherol (vitamin E) (icr) 10% pet. Tolazoline 10% aq. Tolnaftate 1% pet. Toluene-2,5-diamine (p-toluenediamine) (PTD) 1% pet. + + + Toluene diisocyanate (TDI) 2% pet. + 1% pet. Toluenesulfonamide/formaldehyde resin See Tosylamide/formaldehyde resin Toluenesulfonhydrazide 0.5% alc. 4-Tolyldiethanolamine 2% pet. Tosylamide/formaldehyde resin (toluene- 10% pet. + + + sulfonamide/formaldehyde resin) Triamcinolone acetonide 1% alc. 0.1% pet. 1% pet. 0.1% pet. Tribromsalan (TBS) (ph) 1% pet. + Trichloroethylene 5% o.o. (TCC) (ph) 1% pet. + + Triclosan (ph) 2% pet. + + + Tricresyl phosphate 5% pet. + + + Triethanolamine 2.5% pet. + 2% pet. + Triethanolamine polypeptide oleate condensate 25% o.o (Xerumenex®) Triethyleneglycol diacrylate (TREGDA) 0.1% pet. + Triethyleneglycol dimethacrylate (TREGDMA) 2% pet. + + + Triethylenetetramine (TETA) 0.5% pet. + + + Trifluridine 5% pet. Triflusal (2-hydroxy-4-trifluoro-methylbenzoic 1% pet. acid) (phde) Triglycidyl isocyanurate 0.5% pet. + Trimeprazine tartrate (alimemazine tartrate) 1% pet. Trimethoprim (de) (fde) 10% alc. or pet. 2,2,4-Trimethyl-1,2-dihydroquinoline 1% pet. + Trimethylolpropane triacrylate (TMPTA) 0.1% pet. + Tripelennamine 1% pet. Triphenyl phosphate 5% pet. + + + Tripropyleneglycol diacrylate (TPGDA) 0.1% pet. + 1,3,5-Tris(2-hydroxyethyl)-hexahydrotriazine 1% pet.1% aq. (Grotan® BK) Tris(hydroxymethyl)nitromethane (tris nitro) 1% pet. + + + Tris nitro See Tris(hydroxymethyl)nitromethane Trolamine See Triethanolamine Tromantadine hydrochloride 1% pet. Tropicamide (icr) 1% pet. TSS Agfa® See Amino-4-N, N-diethylaniline sulfate Tuberculin (bovine) 10% aq. Tulipalin See α-Methylene-γ-butyrolactone Turpentine oil (icr) 10% pet. + +; 20% pet. Turpentine peroxides (icr) 0.3% o.o. 0.3% pet. Tylosin tartrate 5% pet. Undecylenic acid 2% pet. Urea formaldehyde resin 10% pet. + Urethane diacrylate (aliphatic) 0.1% pet. + 49_907_928 05.11.2005 12:13 Uhr Seite 926

926 Anton C. De Groot, Peter J. Frosch

Table 2. Continued

Allergen Test Concentration and vehicle Suppliers

Trolab Chemo Brial

Urethane diacrylate (aromatic) 0.05% pet. + Urethane dimethacrylate (UEDMA) 2% pet. + + Usnic acid (ph) 0.1% pet. + + + Valaciclovir 10% aq. Vanillin (icr) 10% pet. + + + Vat green 1 (CI 59825) 1% pet. Vesuvine brown See Bismarck Brown 49 Virginiamycin (de) (icr) 5% pet. Vitamin A acetate 1% pet. Vitamin E See α-Tocopherol Vitamin K (K1, K3, K4) (de) 0.1% pet. Warfarin 0.5% pet. Wood mix (pine, spruce, birch, teak) 20% pet. Wood tar mix (pine, beech, juniper, birch) (ph) 12% pet. 12% pet. Wool wax alcohols See Lanolin alcohol Xerumenex® See Triethanolamine polypeptide oleate condensate Ya r row S e e Achillea millefolium Ylang-ylang oil See Cananga odorata Zinc acexamate (de) 5% aq. Zinc chloride (icr) 1% pet. 1% pet. Zinc dibutyldithiocarbamate (ZBC) (icr) 1% pet + + + Zinc diethyldithiocarbamate (ZDC) (icr) 1% pet. + + + Zinc dimethyldithiocarbamate (Ziram) (icr) 1% pet. + Zinc ethylenebis(dithiocarbamate) See Zineb Zinc (powder) 2.5% pet. 1% pet. Zinc pyrithione (ph) 1% pet. 1% pet. 0.1% pet. Zineb [zinc ethylenebis(dithiocarbamate)] (ph) 1% pet. + + Ziram See Zinc dimethyldithiocarbamate Zirconium oxide 0.1% pet. 49_907_928 05.11.2005 12:13 Uhr Seite 927

Patch Test Concentrations and Vehicles Chapter 49 927

Table 3. Recommended test concentrations for groups of chemicals. (acet. Acetone, alc. alcohol, aq. water, pet. petrolatum.) [icr Immediate contact reactions reported (Chaps. 5 and 26), de drug eruption with positive patch test reported (Chap. 24), fde fixed drug eruption with positive patch test reported (Chap. 24), ph photosensitivity reported (Chaps. 6, 17 and 27), phde photosensitive drug eruption with positive photopatch test reported (Chaps. 6, 17, 24 and 27)]

Product Test concentration and vehicle

Acrylates, monoacrylates (icr) 0.1% pet. Aminoglycosides (de) 20% pet. Anesthetics, local 0.5–2% aq. Barbiturates (fde) 10% alc. or pet. Benzodiazepines (de) 1% aqua; 5–10% pet; 5–20% pet or aq. Beta-lactam antibiotics (de) 1–20% pet. Carbowaxes Pure Cephalosporins (de) (icr) 10%–20% pet. or 1%–10% aq. Epoxy resins (DGEBA based) 1% pet. Epoxy resins (not DGEBA based) 0.25–1% pet. Epoxy resin hardeners 0.1–1% in pet., acet. or alc. Epoxy resin reactive diluents 0.1–1% in pet., acet. or alc. Heparins (de) Pure Imidazole antimycotics 1% alc. Methacrylates, monomers 2% pet. Penicillins (de) 1–20% pet. Phenothiazine derivatives (fde) 1–10% pet. or 10% alc. Phytotherapeutics (de) Pure and 10% Polyethylene glycols (icr) Pure Quaternary ammonium compounds 0.1% aq. 4-Quinolines (phde) 10% pet. Sulfonamides (de) (fde) 10% alc. or pet. Tetracyclines 3% pet. Triphenylmethane dyes 2% aq. 49_907_928 05.11.2005 12:13 Uhr Seite 928

928 Anton C. De Groot, Peter J. Frosch

Table 4. List of abbreviations

ANTU 1-Naphthalenylthiourea HPMA 2-Hydroxypropyl methacrylate BA Butyl acrylate HQ Hydroquinone BAK Benzalkonium chloride IBA Isobornyl acrylate BIS-EMA 2,2-bis[4-(2-Methacryloxyethoxy)phe- IPD Isophorone diamine nyl]propane IPDI Isophorone diisocyanate BIS-GA 2,2-bis[4-(2-hydroxy-3-acryloxyprop- IPPD N-Isopropyl-N´-phenyl-p-phenylenedia- oxy)phenyl]propane (epoxy diacrylate) mine BIS-GMA 2,2-bis[4-(2-Hydroxy-3-methacryloxyprop- MBAA N, N-Methylenebisacrylamid oxy)phenylpropane] MBT Mercaptobenzothiazole BIS-MA 2,2-bis[4-(Methacryloxy)phenyl]propane 49 MBTS Dibenzothiazyl disulfide BIT Benzisothiazolinone 6-MC Methylcoumarin BMA n-Butyl methacrylate MCPA Chloromethylphenoxyacetic acid BUDA 1,4-Butanediol diacrylate MDI Diphenylmethane-4, 4-diisocyanate BUDMA 1,4-Butanediol dimethacrylate MHQ Methylhydroquinone CBS N-Cyclohexylbenzothiazyl sulfenamide MMA Methyl methacrylate CDAA N, N-Diallyl-2-chloroacetamide MOR Morpholinyl mercaptobenzothiazole CPPD N-Cyclohexyl-N-phenyl-p-phenylenedia- ONPPD 2-Nitro-p-phenylenediamine mine OTA Oligotriacrylate 2,4-D 2,4-Dichlorophenoxyacetic acid PAN Phenyl-azo-2-naphthol 2,4-DB 2,4-Dichlorophenoxybutyric acid PBN Phenyl-β-naphthylamine DBNPD N, N-Di-β-naphthyl-p-phenylenediamine PCMC p-Chloro-m-cresol DDAG Dodecyl di(aminoethyl)glycine PCMX Chloroxylenol DDT Dichlorodiphenyltrichloroethane PCP Pentachlorophenol DDVP Dichlorvos PEA 2-Phenoxyethyl acrylate DEGDA Diethyleneglycol diacrylate PETA Pentaerythritol triacrylate DEHP Di-2-ethylhexyl phthalate (=dioctyl phthalate) P-F-R-2 Phenol-formaldehyde resin DETA Diethylenetriamine PPP Phenylpropylpyridine DMAEMA N, N-Dimethylaminoethyl methacrylate PTBC p-tert-Butylcatechol DNCB 2,4-Dinitrochlorobenzene PTBT p-tert-Butylphenol-formaldehyde resin DNOC 4,6-Dinitrocresol PTD Toluene-2,5-diamine DOP Dioctyl phthalate 2,4,5-T 2,4,5-Trichlorophenoxyacetic acid DPG 1,3-Diphenylguanidine TBS Tribromsalan DPGDA Dipropyleneglycol diacrylate TCA Sodium thichloroacetate DPPD N, N´-Diphenyl-p-phenylenediamine TCC Triclocarban DPTU Diphenylthiourea TCSA Tetrachlorosalicylanilide EA Ethyl acrylate TDI Toluene diisocyanate EBADMA Ethoxylated bisphenol-A dimethacrylate TETA Triethylenetetramine ECA Ethyl cyanoacrylate TETD Tetraethylthiuram disulfide EDTA Ethylenediaminetetraacetic acid disodium TGIC Tetraglycidyl isocyanaurate salt (see disodium EDTA) THFMA Tetrahydrofurfuryl methacrylate EEA Ethoxyethyl acrylate TMPTA Trimethylolpropane triacrylate EGDMA Ethyleneglycol dimethacrylate TMTD Tetramethylthiuram disulfide EHA 2-Ethylhexyl acrylate TMTM Tetramethylthiuram monosulfide EMA Ethyl methacrylate TPGDA Tripropyleneglycol diacrylate GMA Glycidyl methacrylate TREGDA Triethyleneglycol diacrylate HCH Hexachlorocyclohexane TREGDMA Triethyleneglycol dimethacrylate HDDA 1,6-Hexanediol diacrylate TSS 4-Amino-N, N-dietyhylaniline sulfate HDI Hexamethylenediisocyanate UDA Urethane diacrylate 2-HEA 2-Hydroxyethyl acrylate UDMA Urethane dimethacrylate HEMA 2-Hydroxyethyl methacrylate ZBC Zinc dibutyldithiocarbamate HPA 2-Hydroxypropyl acrylate ZDC Zinc diethyldithiocarbamate