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Granulation in Florida Citrus

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Granulation in Florida Citrus Literature Cited harvest modulate the severity of postharvest peel pitting in citrus. J. Amer. Soc. Hort. Sci. (In press). Agusti, M., V. Almela, M. Juan, F. Alferez, F. R. Tadeo, and L. Zacarias. 2001. Lafuente, M. T. and J. M. Sala. 2002. Abscisic acid and the influence of ethyl- Histological and physiological characterization of rind breakdown of Na- ene, humidity and temperature on the incidence of postharvest rindstain- velate sweet orange. Ann. Bot. 88:422-451. ing of Navelina oranges (Citrus sinensis L. Osbeck) fruits. Postharvest Biol. Alferez, F., M. Agusti, and L. Zacarias. 2003. Postharvest rind staining in Na- Technol. 25:49-57. vel oranges is aggravated by changes in storage relative humidity: effect Petracek, P. D., L. Montalvo, H. Dou, and C. Davis. 1998. Postharvest pitting on respiration, ethylene production and water potential. Postharvest Bi- of ‘Fallglo’ tangerine. J. Amer. Soc. Hort. Sci. 123: 130-135. ol. Technol. 28:143-152. Petracek, P. D., W. F. Wardowsky, and G. E. Brown. 1995. Pitting of grapefruit Alferez, F. and J. Burns. 2004. Postharvest peel pitting at non-chilling temper- that resembles chilling injury. HortScience 30:1422-1426. atures in grapefruit is promoted by changes from low to high relative hu- Shomer, I. and Y. Erner. 1989. The nature of oleocellosis in citrus fruits. Bot. midity during storage. Postharvest Biol. Technol. 32:79-87. Gazette 50:281-288. Alferez, F., L. Zacarias, and J. Burns. 2004. Cumulative hours of low relative humidity before storage at high relative humidity and relative humidity at Proc. Fla. State Hort. Soc. 117:358-361. 2004. GRANULATION IN FLORIDA CITRUS MARK A. RITENOUR1 but specific data and their related impact on granulation in University of Florida Florida are limited. Indian River Research and Education Center Ft. Pierce, FL 34945-3138 What is Granulation? L. GENE ALBRIGO, JACQUELINE K. BURNS Granulation (also called crystallization or section drying) AND WILLIAM M. MILLER is a physiological disorder in citrus that results in reduced University of Florida extractable juice and sometimes vesicle shriveling (Fig. 1). Citrus Research and Education Center While segments appear dry, the disorder is not caused by dry- Lake Alfred, FL 33850-2299 ing, but by gel formation within the vesicles (Bartholomew et al., 1941). Freezing and sunburn cause injury that can be mistaken for granulation, but these events cause cellular col- Additional index words. physiological disorder, section drying, navel orange, fruit density, temperature, rainfall Abstract. Granulation (also called crystallization or section dry- ing) is a physiological disorder in citrus that results in reduced extractable juice and sometimes vesicle shriveling. While seg- ments appear dry, the disorder is not caused by drying, but by gel formation within the vesicles. Though many citrus varieties may develop granulation (i.e., ‘Valencia’ orange, tangerines, and grapefruit), it was a particular problem in Florida ‘Navel’ oranges during the 2003 season. Many factors have been as- sociated with the development of granulation in citrus, includ- ing advanced fruit maturity, large fruit, excessive tree vigor, severe mite damage, composition of the juice, and cool, dry, windy weather conditions. Tree water status and irrigation have also been reported to affect granulation with researchers reporting less granulation with less irrigation. During the 2003 navel orange season, the relatively high temperatures during bloom, low fruit set, and associated larger fruit likely played an important role in the excessive development of granulation in the fruit. Changing cultural practices (i.e., fertilization and irri- gation) and use of rootstocks that encourage vigorous tree growth may have promoted the development of granulation, This research was supported by the Florida Agricultural Experiment Sta- tion, and approved for publication as Journal Series No. N-02583. Trade and company names are included for benefit of reader and imply no endorse- ment or preferential treatment of products by the University of Florida. 1Corresponding author. Fig. 1. Granulation of navel orange. 358 Proc. Fla. State Hort. Soc. 117: 2004. lapse and localized tissue death resulting in loss of juice and in the fruit. Production of large fruit is often associated with subsequent drying of sections, and not gel formation. Injury low fruit set which results in more plant resources (e.g., car- from freezing or sunburn can be separated from sound fruit bohydrates) available to each fruit for growth. Thus, delaying based on fruit density and water content using optical sizers/ harvest of trees producing a small number of large fruit, graders that can calculate density based on fruit shape, or by should be avoided. Comparing alternate bearing cycles of cit- the use of near infrared (NIR) absorption spectrometry or X- rus, El-Zeftawi (1973) reported more granulation during the ray computed tomography (Grierson and Hayward, 1959; light-bearing (“off”) years, compared to the heavy-bearing Miller, et al., 1988; Peiris et al., 1998). Even in granulated (“on”) years. Young trees often also experience greater levels grapefruit, however, there is often a mixture of granulated of granulation (Awasthi and Nauriyal, 1972a; Bartholomew vesicles and desiccated, collapsed vesicles that make definite et al., 1941) possibly due to their rapid growth (vigor) and distinction between granulation and vesicle desiccation diffi- production of fewer, but larger fruit. cult, but that allows possible separation of unmarketable fruit Growing location (i.e., higher levels in the coastal regions based on fruit density (Hwang et al., 1988). of California compared to the interior) and rootstock have Granulated vesicles within sections are discolored with a been reported to influence the development of granulation tough texture. The individual parenchyma cells within gran- (Awasthi and Nauriyal, 1972a; Bartholomew et al., 1941; El- ulated vesicles have thickened walls with secondary wall for- Zeftawi, 1978), but results vary with growing region. Awasthi mation in severe cases (Burns and Achor, 1989; Hwang et al., and Nauriyal (1972a) also reported that granulation was en- 1990). Such changes involve increased concentrations of var- hanced under shaded conditions, being highest in interior- ious cell wall components (cellulose, hemicellulose, pectin, canopy fruit and in fruit covered with black bags. In addition, and lignin). Granulated vesicles also have elevated respira- granulation is associated more with late-bloom fruit (Bartho- tion, increased juice pH, and less soluble sugars and acids lomew et al., 1941) than fruit from the main bloom. (Bartholomew et al., 1941; Burns, 1990; El-Zeftawi, 1978; Gil- Tree water status and irrigation have been reported to af- fillan and Stevenson, 1977; Sinclair and Jolliffe, 1961). In- fect granulation with researchers reporting less granulation creased respiration is thought to fuel the various metabolic with less irrigation (Bartholomew et al., 1941). This effect ap- changes, especially changes in the cell wall (Burns, 1990). pears to be independent of fruit size. In one block of severely Other compositional changes are also evident within granu- granulated Florida ‘Valencia’ orange, 90% of fruit from trees lated tissue, with granulated juice vesicles containing 1.7 receiving irrigation during drought periods developed at times the magnesium and more than twice the calcium con- least some granulation, compared to only 72% of the fruit tent of normal vesicles (Sinclair and Jolliffe, 1961). It is from unirrigated trees (Sites et al., 1951). Furthermore, in thought that elevated levels of pectin and calcium result in South African ‘Navel’ oranges, researchers reported that the gel formation characteristic of granulated tissue. heavy late-summer rains enhanced granulation (Noort, Many citrus cultivars develop granulation such as ‘Valen- 1969). Severe mite damage, and cool, dry, windy weather con- cia’ and navel orange (Bartholomew et al., 1941; El-Zeftawi, ditions have also been mentioned as possible causes of gran- 1978; Gilfillan and Stevenson, 1977; Noort, 1969), tangerines ulation (Browning et al., 1995). (Nakajima, 1976), and grapefruit (Burns and Albrigo, 1997; As mentioned earlier, granulation is associated with lower Hwang et al., 1988). However, the disorder develops differ- sugar and acid levels within the fruit. Because many of the fac- ently depending on the citrus species: in navel oranges, gran- tors mentioned above relating to granulation also result in re- ulation often extends through the center of the fruit duced internal sugar and/or acid content (e.g., late-bloom (Gilfillan and Stevenson, 1977; Noort, 1969); in grapefruit, it fruit, large fruit, high temperatures, increased fruit water con- develops first at the stylar-end of the fruit (Burns and Albrigo, tent through irrigation or rain, shaded conditions, etc.), un- 1997); and in the other types, it develops first at the stem end usually low sugar and acid content may somehow provide a (Bartholomew et al., 1941). unifying mechanism for the development of granulation. Possible Causes of Granulation Florida ‘Navel’ Oranges this Season Though granulation has been shown to develop during While granulation often develops in late-season navel or- storage in some citrus regions of the world (Gilfillan and anges, the 2003 Florida ‘Navel’ orange season experienced se- Stevenson, 1977; Noort, 1969), in the United States it is con- vere (50%, 75% or more) granulation relatively
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