© 2021. Published by The Company of Biologists Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by /4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. A general role for MIA3/TANGO1 in secretory pathway organization and function Janine McCaughey1†, Nicola L. Stevenson1, Judith M. Mantell2, Chris R. Neal2, Alex Paterson3, Kate Heesom4, and David J. Stephens1* 1Cell Biology Laboratories, School of Biochemistry, Faculty of Life Sciences, University Walk, University of Bristol, Bristol, BS8 1TD, UK. 2Wolfson Bioimaging Facility, Faculty of Life Sciences, University Walk, University of Bristol, Bristol, BS8 1TD, UK. 3 In Silico Consulting, Winchester, UK. 4Proteomics Facility, Faculty of Life Sciences, University Walk, University of Bristol, Bristol, BS8 1TD, UK. *Correspondence to:
[email protected]. †Current address: Utrecht University, Kruytgebouw, Padualaan 8, 3584 CH Utrecht, The Netherlands ORCID: JM: 0000-0001-7709-2597 NS: 0000-0001-8967-7277 JMM: 0000-0002-9061-8933 CRN: 0000-0001-6604-280X AP: 0000-0003-3025-8347 KH: 0000-0002-5418-5392 DJS: 0000-0001-5297-3240 Keywords: Secretory pathway, COPII, collagen, Golgi, TANGO1, ERGIC Summary statement: TANGO1 is required to maintain the fundamental organization of the early secretory pathway in mammalian cells to support secretion of a diverse range of newly synthesized cargo proteins. Abstract Complex machinery is required to drive secretory cargo export from the endoplasmic reticulum, an essential process in eukaryotic cells. In vertebrates, the Mia3 gene encodes two major forms of Transport ANd Golgi Organization Protein 1 (TANGO1S and TANGO1L), previously implicated in selective trafficking of procollagen.