Anti-HIC2 Antibody (BPE) Product Number: AC21-2724-08

Overview Host Species: Goat Clonality: Polyclonal Purity: Affinity purified using the immunizing peptide. The affinity purified antibody is then conjugated to B-Phycoerythrin, also known as BPE or B-PE, which is a form of the photosynthesis protein phycoerythrin (PE). BPE has less nonspecific staining than it’s “R” counterpart, RPE in some fluorescent assays. Conjugate: BPE Immunogen Type: HIC2 antibody (BPE) was raised against a synthetic peptide of HIC2. The HIC2 peptide immunogen had the following sequence: C-PQELPQAKGSDDE Physical Characteristics Amount: 0.05 mg Concentration: 0.5 mg/ml Volume: 0.1 ml Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Storage: B-PE conjugated antibody can be stored at 4?C for up to 18 months. For longer storage the conjugate can be stored at -20?C after adding 50% glycerol. B-PE conjugated antibodies should always be stored in the dark. Specificity Predicted Species Reactivity: Human, Mouse, Rat, Dog, Pig, Cow Target Information Accession Number(s): NP_055909.2 Gene ID Number(s): 23119 (human), 58180 (mouse), 287940 (rat) Alternative Names: HIC1 related gene on chromosome 22; Hic3; HRG22; Hypermethylated in cancer 2 protein; ZBTB30; Zinc finger and BTB domain-containing protein 30; ZNF907 Application Details Blocking Peptide: HIC2 peptide, catalog number AC21-2724-P, is the immunizing peptide for this product and can be used as a negative control to block antibody (BPE) binding in a competition or adsorption assay. Tested Applications: ELISA, WB ELISA: Detection limit dilution 1:128000. Western Blot: Recommended starting concentration for western blot is 0.1-0.3µg/ml.

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