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THAT ARE NOT ALLOKULUNUTTUUS009816094B2 (12 ) United States Patent ( 10) Patent No THAT ARE NOT ALLOKULUNUTTUUS009816094B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,816 , 094 B2 Lee et al. (45 ) Date of Patent: * Nov . 14 , 2017 ( 54 ) POLYCOMB- ASSOCIATED NON -CODING ( 2013 .01 ) ; C12Q 2600 / 136 (2013 . 01 ) ; C12Q RNAS 2600/ 158 ( 2013 . 01 ) ; C12Q 2600 /178 (2013 .01 ) (71 ) Applicant : The General Hospital Corporation , ( 58 ) Field of Classification Search Boston , MA (US ) None See application file for complete search history. (72 ) Inventors : Jeannie T . Lee , Cambridge, MA (US ) ; Jing Zhao , San Diego , CA (US ) ; (56 ) References Cited Kavitha Sarma, Waltham , MA (US ) ; Mark Borowsky , Needham , MA (US ) ; U . S . PATENT DOCUMENTS Toshiro Kendrick Ohsumi, Cambridge , 5 ,491 , 133 A 2 / 1996 Walder et al. MA (US ) 5 , 576 , 208 A 11 / 1996 Monia et al. 5 ,623 , 065 A 4 / 1997 Cook et al . ( 73 ) Assignee : The General Hospital Corporation , 5 ,652 , 355 A 7 / 1997 Metelev et al. 5 ,661 , 134 A 8 / 1997 Cook et al. Boston , MA (US ) 5 ,914 , 396 A 6 / 1999 Cook et al . 5 ,919 ,619 A 7 / 1999 Tullis ( * ) Notice : Subject to any disclaimer , the term of this 5 , 965 , 722 A 10 / 1999 Ecker et al. patent is extended or adjusted under 35 5 , 976 ,879 A 11/ 1999 Kole et al. U . S . C . 154 (b ) by 0 days. 6 ,015 , 710 A 1 / 2000 Shay et al . 6 ,040 , 142 A 3 / 2000 Melki et al. This patent is subject to a terminal dis 6 ,046 , 307 A 4 / 2000 Shay et al . claimer . 6 ,080 , 577 A 6 / 2000 Melki et al . 6 , 107 ,094 A 8 / 2000 Crooke 6 , 143 ,881 A 11/ 2000 Metelev et al. (21 ) Appl. No .: 15 / 171, 706 6 , 146 , 829 A 11/ 2000 Cook et al . 6 , 197, 944 B1 . 3 / 2001 Walder et al. ( 22 ) Filed : Jun . 2 , 2016 6 , 210, 892 B1 4 / 2001 Bennett et al. 6 , 294, 650 B1 9 / 2001 Shay et al . (65 ) Prior Publication Data 6 , 326 , 199 B1 12 / 2001 Cook et al. 6 ,346 , 614 B1 2 /2002 Metelev et al. US 2016 /0355813 A1 Dec . 8 , 2016 6 , 359 ,124 B1 3 / 2002 Ecker et al . 6 ,451 , 991 B1 9 / 2002 Martin et al. 6 , 531 , 584 B1 3 / 2003 Cook et al. Related U .S . Application Data 6 , 608 , 035 B1 8 /2003 Agrawal et al . (63 ) Continuation of application No . 15 /050 , 273 , filed on 6 , 653 , 466 B2 11/ 2003 Matsuo Feb . 22, 2016 , which is a continuation of application 6 ,653 , 467 B1 11 /2003 Matsuo et al. No . 13 /884 ,670 , filed as application No . (Continued ) PCT/ US2011 /060493 on Nov . 12 , 2011 , now Pat. No. 9 ,328 , 346 . FOREIGN PATENT DOCUMENTS CA 2805791 1 /2012 (60 ) Provisional application No . 61 /412 , 862 , filed on Nov. EP 0 999 270 5 / 2000 12 , 2010 , provisional application No . 61/ 425 , 174 , ( Continued ) filed on Dec . 20 , 2010 , provisional application No . 61/ 512 , 754 , filed on Jul. 28 , 2011 . OTHER PUBLICATIONS (51 ) Int. CI. Behlke et al , Designing Antisense Oligonucleotides, 2005 , Inte C12N 15 / 11 ( 2006 . 01 ) grated DNA Technologies , pp . 1 - 17 . * C12N 15 / 113 ( 2010 .01 ) Davidovich , The recruitment of chromatin modifiers by long noncoding RNAs : lessons from PRC2 , 2015 , RNA , 21 : 2007 C120 1 /68 ( 2006 .01 ) 2022 . * A61K 9 / 127 ( 2006 .01 ) International Search Report and Written Opinion for Application A61K 31/ 713 ( 2006 .01 ) No. PCT/ US2011 / 060493 dated Apr. 18 , 2012 , 17 pages . (52 ) U .S . CI. International Search Report and Written Opinion for Application CPC . .. .. CI2N 15 / 1137 ( 2013 . 01 ) ; A61K 9 / 1271 No . PCT/ US2013 / 041440 dated Jul. 29 , 2013 , 17 pages . ( 2013 .01 ) ; A61K 31/ 713 ( 2013 .01 ) ; C12N International Search Report and Written Opinion for Application 15 / 113 ( 2013 .01 ) ; C12N 15 / 1135 ( 2013 .01 ) ; No . PCT/US2013 /041452 dated Jul. 29 , 2013 , 15 pages . C12N 15 / 1136 ( 2013 .01 ) ; C12Q 1 /6876 ( Continued ) ( 2013 .01 ) ; C12Q 1 /6881 ( 2013 .01 ) ; C120 1 /6886 ( 2013 .01 ) ; C12Y 201701043 (2013 .01 ) ; Primary Examiner — Kate Poliakova -Georgantas C12N 2310 / 113 ( 2013 .01 ) ; C12N 2310 / 14 ( 74 ) Attorney , Agent, or Firm — Fish & Richardson P . C . (2013 .01 ) ; C12N 2310 / 141 ( 2013 .01 ) ; CI2N 2310 / 314 ( 2013 .01 ) ; CI2N 2310 / 315 (57 ) ABSTRACT ( 2013 .01 ) ; C12N 2310 / 3181 ( 2013 .01 ) ; C12N This invention relates to long non -coding RNAs ( lncRNAs ) , 2310 /321 ( 2013. 01 ) ; C12N 2310 /3231 libraries of those ncRNAs that bind chromatin modifiers , (2013 . 01 ) ; C12N 2310 /3233 ( 2013 .01 ) ; C12N such as Polycomb Repressive Complex 2 , inhibitory nucleic 2310 /346 (2013 .01 ) ; CI2N 2320 / 30 ( 2013 . 01 ) ; acids and methods and compositions for targeting lncRNAs. 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