THAT ARE NOT ALLOKULUNUTTUUS009816094B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,816 , 094 B2 Lee et al. (45 ) Date of Patent: * Nov . 14 , 2017 ( 54 ) POLYCOMB- ASSOCIATED NON -CODING ( 2013 .01 ) ; C12Q 2600 / 136 (2013 . 01 ) ; C12Q RNAS 2600/ 158 ( 2013 . 01 ) ; C12Q 2600 /178 (2013 .01 ) (71 ) Applicant : The General Hospital Corporation , ( 58 ) Field of Classification Search Boston , MA (US ) None See application file for complete search history. (72 ) Inventors : Jeannie T . Lee , Cambridge, MA (US ) ; Jing Zhao , San Diego , CA (US ) ; (56 ) References Cited Kavitha Sarma, Waltham , MA (US ) ; Mark Borowsky , Needham , MA (US ) ; U . S . PATENT DOCUMENTS Toshiro Kendrick Ohsumi, Cambridge , 5 ,491 , 133 A 2 / 1996 Walder et al. MA (US ) 5 , 576 , 208 A 11 / 1996 Monia et al. 5 ,623 , 065 A 4 / 1997 Cook et al . ( 73 ) Assignee : The General Hospital Corporation , 5 ,652 , 355 A 7 / 1997 Metelev et al. 5 ,661 , 134 A 8 / 1997 Cook et al. 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Behlke et al , Designing Antisense Oligonucleotides, 2005 , Inte C12N 15 / 11 ( 2006 . 01 ) grated DNA Technologies , pp . 1 - 17 . * C12N 15 / 113 ( 2010 .01 ) Davidovich , The recruitment of chromatin modifiers by long noncoding RNAs : lessons from PRC2 , 2015 , RNA , 21 : 2007 C120 1 /68 ( 2006 .01 ) 2022 . * A61K 9 / 127 ( 2006 .01 ) International Search Report and Written Opinion for Application A61K 31/ 713 ( 2006 .01 ) No. PCT/ US2011 / 060493 dated Apr. 18 , 2012 , 17 pages . (52 ) U .S . CI. International Search Report and Written Opinion for Application CPC . .. .. CI2N 15 / 1137 ( 2013 . 01 ) ; A61K 9 / 1271 No . PCT/ US2013 / 041440 dated Jul. 29 , 2013 , 17 pages . ( 2013 .01 ) ; A61K 31/ 713 ( 2013 .01 ) ; C12N International Search Report and Written Opinion for Application 15 / 113 ( 2013 .01 ) ; C12N 15 / 1135 ( 2013 .01 ) ; No . PCT/US2013 /041452 dated Jul. 29 , 2013 , 15 pages . C12N 15 / 1136 ( 2013 .01 ) ; C12Q 1 /6876 ( Continued ) ( 2013 .01 ) ; C12Q 1 /6881 ( 2013 .01 ) ; C120 1 /6886 ( 2013 .01 ) ; C12Y 201701043 (2013 .01 ) ; Primary Examiner — Kate Poliakova -Georgantas C12N 2310 / 113 ( 2013 .01 ) ; C12N 2310 / 14 ( 74 ) Attorney , Agent, or Firm — Fish & Richardson P . C . (2013 .01 ) ; C12N 2310 / 141 ( 2013 .01 ) ; CI2N 2310 / 314 ( 2013 .01 ) ; CI2N 2310 / 315 (57 ) ABSTRACT ( 2013 .01 ) ; C12N 2310 / 3181 ( 2013 .01 ) ; C12N This invention relates to long non -coding RNAs ( lncRNAs ) , 2310 /321 ( 2013. 01 ) ; C12N 2310 /3231 libraries of those ncRNAs that bind chromatin modifiers , (2013 . 01 ) ; C12N 2310 /3233 ( 2013 .01 ) ; C12N such as Polycomb Repressive Complex 2 , inhibitory nucleic 2310 /346 (2013 .01 ) ; CI2N 2320 / 30 ( 2013 . 01 ) ; acids and methods and compositions for targeting lncRNAs. 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