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Malformaciones Esqueléticas En Larvas De Lenguado Senegalés

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Malformaciones Esqueléticas En Larvas De Lenguado Senegalés Gomes Doctoral Thesis de Azevedo de Ana Manuela Ana of Summary This Doctoral Thesis arises from the need to find solutions to prevent the high incidence of skeletal abnormalities detected in Senegalese sole (Solea senegalensis) aquaculture. A comprehensive study of skeletal anomalies production affecting the vertebral column of Senegalese sole was performed at different rearing stages and feeding regimes. Complementary diagnostic the methodologies were integrated, from the macroscopic, stereoscopic, radiographic and histologic perspective. The present Thesis contributed with of a new insight on the skeletal anomaly problematic affecting cultured Senegalese sole throughout the productive cycle, underlining the importance of an interdisciplinary approach to cope a multi-factorial issue in the phases aquaculture sector. Resumen CHARACTERIZATION OF THE VERTEBRAL ANOMALIES IN Esta Tesis Doctoral surge de la necesidad de encontrar soluciones eficaces approach histological and radiographic ): stereoscopic, para reducir la alta incidencia de anomalías esqueléticas detectada en la DIFFERENT PHASES OF THE PRODUCTION OF SENEGALESE producción de lenguado senegalés (Solea senegalensis). Se realizó un estudio exhaustivo de las anomalías esqueléticas que afectaban la columna vertebral SOLE (SOLEA SENEGALENSIS): STEREOSCOPIC, del lenguado senegalés en diferentes etapas de cultivo y sometidos a vertebral anomalies in different senegalensis RADIOGRAPHIC AND HISTOLOGICAL APPROACH distintas dietas. Para ello se han integrado metodologías de diagnóstico the complementarias, desde el punto de vista macroscópico, estereoscópico, olea Ana Manuela de Azevedo Gomes S radiográfico e histológico. La presente Tesis contribuyó con una nueva visión ( de la problemática de las anomalías esqueléticas que afectan al lenguado sole senegalés a lo largo del ciclo productivo. Este trabajo subraya la importancia de un abordaje interdisciplinario para hacer frente a un problema multifactorial en el sector de la acuicultura. enegalese Characterization Characterization of S PROGRAMA DE DOUTORAMENTO EN INVESTIGACIÓN BÁSICA E APLICADA EN CIENCIAS VETERINARIAS Lugo DEPARTAMENTO DE ANATOMÍA, PRODUCIÓN ANIMAL E CIENCIAS CLÍNICAS VETERINARIAS FACULTADE DE VETERINARIA 2017 DOCTORAL THESIS COMPENDIUM OF ARTICLES CHARACTERIZATION OF THE VERTEBRAL ANOMALIES IN DIFFERENT PHASES OF THE PRODUCTION OF SENEGALESE SOLE (SOLEA SENEGALENSIS): STEREOSCOPIC, RADIOGRAPHIC AND HISTOLOGICAL APPROACH Caracterización de las anomalías vertebrales en distintas fases de producción del lenguado senegalés (Solea senegalensis): estudio estereoscópico, radiográfico e histológico Sgd: Ana Manuela de Azevedo Gomes DEPARTAMENTO DE ANATOMÍA, PRODUCIÓN ANIMAL E CIENCIAS CLÍNICAS VETERINARIAS PROGRAMA DE DOUTORAMENTO EN INVESTIGACIÓN BÁSICA E APLICADA EN CIENCIAS VETERINARIAS FACULTADE DE VETERINARIA LUGO 2017 AUTHORIZATION OF THE DIRECTORS María Isabel Quiroga Berdeal Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) Sonia Vázquez Rodríguez Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) Andrés Barreiro Lois Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) As Directors of the Doctoral Thesis entitled “Characterization of the vertebral anomalies in different phases of the production of Senegalese sole (Solea senegalensis): stereoscopic, radiographic and histological approach” Presented by Ana Manuela de Azevedo Gomes Student from Programa de Doutoramento en Investigación Básica e Aplicada en Ciencias Veterinarias Authorize the presentation of the indicated Thesis, taking into account that it complies with the requirements of article 34 of Regulamento de Estudos de Doutoramento, and as Directors thereof do not incur in the causes of abstention provided for in Ley 30/1992. Autorizan la presentación de la Tesis indicada, teniendo en cuenta que cumple con los requisitos del artículo 34 del Regulamento de Estudos de Doutoramento, y como Directores de la misma no incurren en las causas de abstención previstas en la Ley 30/1992. Lugo, 23 February 2017 Sgd: Mª Isabel Quiroga Berdeal Sgd: Sonia Vázquez Rodríguez Sgd: Andrés Barreiro Lois REPORT OF THE DIRECTORS María Isabel Quiroga Berdeal Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) Sonia Vázquez Rodríguez Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) Andrés Barreiro Lois Associate Professor of the Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias at Universidade de Santiago de Compostela (USC, Spain) As Directors of the Doctoral Thesis entitled “Characterization of the vertebral anomalies in different phases of the production of Senegalese sole (Solea senegalensis): stereoscopic, radiographic and histological approach” We hereby DECLARE: That the Thesis presented by Ms. Ana Manuela de Azevedo Gomes is suitable to be presented, in accordance with article 41 of Regulamento de Estudos de Doutoramento, by the modality of compendium of ARTICLES, in which the doctorate had participation in the weight of the research and its contribution was decisive to carry out this work. And it is known to the coauthors, both doctors and non-doctors, participants in the articles, that none of the papers collected in this Thesis will be presented by any of them in another Doctoral Thesis, which we sign under our responsibility. Por la presente DECLARAMOS: Que la Tesis presentada por Dña. Ana Manuela de Azevedo Gomes es idónea para ser presentada, de acuerdo con el artículo 41 del Regulamento de Estudos de Doutoramento, por la modalidad de compendio de ARTÍCULOS, en los que la doctoranda tuvo participación en el peso de la investigación y su contribución fue decisiva para llevar a cabo este trabajo. Y que son conocedores los coautores, tanto doctores como no doctores, participantes en los artículos, que ninguno de los trabajos reunidos en esta Tesis serán presentados por ninguno de ellos en otra Tesis Doctoral, lo que firmamos bajo nuestra responsabilidad. Lugo, 23 February 2017 Sgd: Mª Isabel Quiroga Berdeal Sgd: Sonia Vázquez Rodríguez Sgd: Andrés Barreiro Lois “… que lo torcido se enderece…” Is 40, 4 To my parents (Aos meus pais) his work was supported by “Consellería de Economía e Industria” of Xunta de Galicia (10MMA020E) and by "Programa de Consolidación e Estructuración de Unidades de Investigación Competitivas GPC2015/034", Spain. Ana Manuela de T Azevedo held a University Professorship Formation (FPU) grant from the Spanish Ministry of Education. The Journal of Comparative Pathology Educational Trust partially supported the stay in Experimental Ecology and Aquaculture Laboratory of the Biology Department of Tor Vergata University (Rome), performed in 2014. ACKNOWLEDGEMENTS I would like to thank with all my heart everyone who directly and indirectly contributed to the realization of this Thesis, in particular: A mis directores de Tesis, Maribel, Sonia y Andrés, por toda la paciencia, esfuerzo y cariño que me brindásteis desde siempre. Gracias por los consejos, el “savoir-faire” y el “savoir-être” que compartís conmigo todos los días y que me dejaron impronta tanto a nivel profesional como humano. Os estaré eternamente agradecida por acogerme tan generosamente, como padres en esta gran familia de “animal CSIs” y de “rayos y truenos”. A la empresa Stolt Sea Farm S.A., especialmente a Ana Riaza y a Isabel Ferreiro, por su disponibilidad, el asesoramiento, el cuidado y suministro de los peces. Gracias por proporcionarnos la oportunidad de contemplar la aplicabilidad de este proyecto. To Professor Clara Boglione, for the exquisite guidance, teaching and good humor during the stay in the Experimental Ecology and Aquaculture Laboratory (LESA) of Tor Vergata University (Rome). I would like also to express my deepest gratitude to her and to LESA collaborators, especially to Drs. Tommaso Russo and Loredana Prestinicola for their kind assistance when preparing and writing the study on Article IV. A los co-autores de los artículos que derivan de esta Tesis Doctoral y a las editoriales John Wiley and Sons e Inter-Research por su colaboración y autorización para reproducir los contenidos de los artículos. Al Hospital Veterinario Universitario Rof Codina, por ceder generosamente sus instalaciones para la realización del estudio radiológico. A Anita, por no dejar nunca de arrimar el hombro, la amistad, el compañerismo y las horas divertidas en la sala de rayos cantando “Bajo el mar...”. Sin duda, mereces ser mi 4ª directora de Tesis. No tengo palabras para agradecértelo. A Meli, Sandra y Dani, por vuestra dedicación, el saber y el afán de mejorar siempre. Gracias por mostrar al mundo la belleza y los colores de este trabajo. A todas las personas que fueron y son parte de la Unidad de Anatomía Patológica: Maribel, Sonia, Anita, Paolo, Sandra, Roberto, Dani, Pedro, Meli, Mónica, Germán, Rita, Xan, Ana y José María. Gracias por formar una “piña” que te integra desde el primer día, por compartir vuestros conocimientos, la alegría y el afecto dentro y fuera del laboratorio. Os agradezco las risas, la sinceridad y la comprensión
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