columns analysis Standard Pretreatment Columns for Column Switching System diagram for column switching Protein removal rate using pretreatment columns columns analysis Standard Method 500 Pretreatment column Protein removal Column rate Features 400 PK-2A 99.62%
PK Effective for both hydrophilic and hydrophobic substances GF-2A* 99.80% 300 Analytical column The high protein removal rate enables efficient pretreatment Injector Company-A 97.68% 200 News No.9, 11, 22
Detector Residual BSA (µg) 100 Pump 1 Pretreatment Columns for Column Switching Method Switching Column for Columns Pretreatment GF-4A Higher protein removal rate than PK columns Method Switching Column for Columns Pretreatment * GF-4A column removes proteins well but is not suitable for trapping hydrophilic substances. 0 Company-A Use PK columns for this purpose. Pump 2 PK-2A GF-2A (Pretreatment) Eluent : 10mM Ammonium acetate buffer(pH7.0) News No.9, 11, 22 Flow rate : 0.5mL/min Column temp. : Room temp. Eluent 1 (Analysis) Column : Shodex PROTEIN KW-604S** Proteins etc. Eluent : 0.1% TFA in (H O/CH CN=50/50) in sample Eluent 2 2 3 Cartridge columns and holder for column switching method Flow rate : 0.5mL/min Detector : UV(280nm) *GF-2A: Custom-made column of Column temp. : Room temp. GF-4A (2.0mmI.D. x 10mm) Switching time : 5min Particle Size Pore Size Column Size **KW-604S is phase-out product. Product Code Product Name (mm) Shipping Solvent Pcs/box (µm) (Å) I.D. x Length
F8700000 MSpak PK-2A 2p 30 30 2.0 x 10 H2O 2 Recovery rate of medical compounds using PK-2A
F8700012 MSpak PK-4A 2p 30 30 4.0 x 10 H2O 2 Recovery Recovery Recovery Sample (%) Sample (%) Sample (%) F8700001 MSpak HLD − − (Holder for PK) − 1 Acetaminophen 115 Cloxazolam 91 Mianserin 92 Base Material : Hydrophilic copolymers containing N-vinyl acetoamide Acetylpheneturide 92 Desipramine 109 Nimetazepam 90 * PK series are cartridge columns and thus should be installed in a column holder "MSpak HLD" before use. Aconitine 110 Diazepam 97 Nitrazepam 97
Alprazolam 99 Diphenhydramine 93 Nortriptyline 86 Column for column switching method Amitriptyline 93 Estazolam 101 Oxazepam 97 Amobarbital 93 Ethenzamide 98 Oxazolam 99 Particle Size Pore Size Column Size Product Code Product Name (mm) Shipping Solvent Barbital 94 Etizolam 105 Pentobarbital 93 (µm) (Å) I.D. x Length Benzoylaconitine 96 Fludiazepam 97 Perfenazine 86
F8700015 MSpak GF-4A 9 400 4.6 × 10 H2O Benzoylhypaconine 83 Flumazenil 93 Phenacetin 108 Biperiden 99 Flunitrazepam 97 Phenobarbital 96 Base Material : Polyvinyl alcohol Bromazepam 102 Flurazepam 106 Phenytoin 99
Bromocriptine 80 Glutethimide 93 Primidone 91
Bromperidol 89 Haloperidol 99 Promethazine 92
Bromvalerylurea 94 Haloxazolam 93 Propericiazine 90
Brotizolam 97 1-Hydroxymethyltriazolam 90 Propranolol 97
Caffeine 106 4-Hydroxytriazolam 91 Secobarbital 97
Carbamazepine 97 Hydroxyzine 99 Sildenafil citrate 95
Carpipramine 99 Hypaconitine 97 Thioridazine 97
Chlordiazepoxide 133 Imipramine 97 Timiperone 88
Chlormezanone 92 Indomethacin 93 Triazolam 96
Chlorpheniramine 111 Levomepromazine 96 Trihexyphenidyl 91
Chlorpromazine 77 Lofepramine 65 Trimethadione 137
Clocapramine 95 Maprotyline 90 Trimipramine 107
Clofedanol 91 Medazepam 91 Warfarin 81
Clomipramine 95 Mephobarbital 99 Zotepine 92
Clonazepam 96 Mesaconine 118
Clotiazepam 96 Metharbital 94
(Adsorption) (Elution) Eluent : 10mM Ammonium acetate buffer(pH7.0) Eluent : 10mM Ammonium acetate buffer(pH7.0)/CH3CN Flow rate : 0.5mL/min Flow rate : 0.5mL/min Detector : UV(220nm) Switching time : 5min 64 65
*Contact Shodex or our distributors near you for customized columns.