Polyclonal Antibody to ZNF687

TA319985 OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

Polyclonal Antibody to ZNF687 (C-term) - Aff - Purified Alternate names: KIAA1441, Zinc finger protein 687 Catalog No.: TA319985 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: The zinc finger protein 687 (ZNF687) was initially identified as a translocation partner gene with RUNX1 in patients with acute myeloid leukemia (AML). Little is known of the function of the ZNF687 protein, but it has been shown to weakly interact with the Ring1/Rnf2 RING finger protein member of the Polycomb group of proteins, suggesting it may be involved in the chromatin-modifying complexes essential for embryonic development and stem cell renewal. Other evidence suggests that ZNF687 may be part of a transcriptional network that also includes ZNF592 and ZMYMD8. Uniprot ID: Q8N1G0 NCBI: NP_065883 GeneID: 57592 Host / Isotype: Rabbit / IgG Immunogen: 17 amino acid synthetic peptide near the carboxy terminus of Human ZNF687 (AP55449CP- N) Format: State: Liquid purified Ig fraction Purification: Affinity chromatography purified via peptide column Buffer System: PBS containing 0.02% Sodium Azide as preservative Applications: Western blot: 0.5-1 µg/ml. Positive Control: Jurkat cell lysate. Immunofluorescence: Start at 20 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody is Human specific. At least three isoforms of ZNF687 are known to exist. This antibody will only detect the largest isoform. Species Reactivity: Tested: Human. Add. Information: Blocking peptide available: AP55449CP-N Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Nguyen TT, Ma LN, Slovak ML, Bangs CD, Cherry AM, Arber DA. Identification of novel Runx1 (AML1) translocation partner genes SH3D19, YTHDf2, and ZNF687 in acute myeloid leukemia. Genes Chromosomes Cancer. 2006 Oct;45(10):918-32. PubMed PMID: 16858696.

For research and in vitro use only. Not for diagnostic or therapeutic work. OG/20160219 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 TA319985: Polyclonal Antibody to ZNF687 (C-term) - Aff - Purified 2. Sánchez C, Sánchez I, Demmers JA, Rodriguez P, Strouboulis J, Vidal M. Proteomics analysis of Ring1B/Rnf2 interactors identifies a novel complex with the Fbxl10/Jhdm1B histone demethylase and the Bcl6 interacting corepressor. Mol Cell Proteomics. 2007 May;6(5):820-34. Epub 2007 Feb 11. PubMed PMID: 17296600. 3. Malovannaya A, Lanz RB, Jung SY, Bulynko Y, Le NT, Chan DW, et al. Analysis of the human endogenous coregulator complexome. Cell. 2011 May 27;145(5):787-99. doi: 10.1016/j.cell.2011.05.006. PubMed PMID: 21620140. Pictures: Western blot analysis of ZNF687 in Jurkat cell lysate with ZNF687 antibody at 0.5 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of ZNF687 in Jurkat cells with ZNF687 antibody at 20 ug/mL.