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下肢慢性皮膚損傷部からkerstersia Gyiorumが検出された 1例

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症例報告 日集中医誌 2017;24:337-40. 下肢慢性皮膚損傷部からKerstersia gyiorumが検出された 1例 杉浦 潤＊1 間藤 卓＊1 関根 進＊2 有馬 史人＊1 大井 秀則＊1 山口 充＊1 中田 一之＊1 杉山 聡＊1 要約：70歳，男性。下肢の慢性皮膚損傷の悪化による重症敗血症にて，近医より紹介となった。 積極的な創部の処置と抗菌薬投与にて敗血症状態を脱し軽快した。後日，創部の培養から， Kerstersia gyiorum（K. gyiorum）が検出された。近年，16S rRNA（16S ribosomal RNA）解析に よる細菌の分類，同定が進んでおり，K. gyiorumは2003年に分類された菌であるが未だ報告例 は乏しい。報告が少ない理由として同定の困難さが挙げられる。今回の症例は，MALDI-TOF MS（matrix assisted laser desorption/ionization time of flight mass spectrometry）法により 同定が行われた。検査機器の進歩に伴い，解析が医療施設で行えるようになったため，これ まで同定が困難であった菌が，比較的容易に同定できるようになり，今後は，今回と同じく， これまで我々があまり聞き慣れない，過去に報告の少ない菌が同定されるケースが増えるこ とが容易に予想される。症例の蓄積と，治療に関する知識の集積が望まれる。 Key words: ①MALDI-TOF MS (matrix assisted laser desorption/ionization time of flight mass spectrometry), ②16S rRNA (16S ribosomal RNA) 下肢の切断が必要と判断されたため，当院に転送され はじめに た。 慢性皮膚損傷とは，6週以上続く，または繰り返す 初診時現症：意識レベルGCS E3V3M6，血圧 皮膚の損傷を呈する病状であり，基礎疾患を有する症 111/61 mmHg，心拍数133 /min整，呼吸数30 /min， 例が多い。感染を合併すると，創傷治癒の遷延や全身 SpO2 97％ （室内気），体温37.6℃，るいそう著明，両 状態の悪化を来す場合がある。今回，下肢の慢性皮膚 側下腿を含め複数箇所に潰瘍を認め，創周囲の発赤と 損傷部にKerstersia gyiorum（K. gyiorum）を起因菌 悪臭を認めた（Fig. 1）。 とする感染を呈した1例を経験した。この菌を起因菌 初診時検査所見：WBC 23,900 /μl，CRP 18.9 mg/ とする症例報告は少ないことから，若干の考察を交え dlと炎症反応の上昇があり，Hb 6.7 g/dlと貧血を認 て報告する。 めた。HbA1cは5.3％と正常値であった。 臨床経過：収容時，意識障害を認め，重症敗血症と 症 例 して治療を開始した。輸液蘇生，赤血球輸血を行い， 症例：70歳，男性。 創部，血液培養を提出後，メロペネム1 gを1日3回， 主訴：全身の痛み。 バンコマイシン1 gを1日2回の投与を開始し，創部は 既往歴：クローン病，ステロイド糖尿病（5年前に通 デブリードマンを施行した。 院を自己中断し未治療）。 翌日には意識レベルはGCS E4V4M6と改善し，創 現病歴：数年前より両下肢に皮膚の潰瘍が出現し， 周囲の炎症所見も改善した。創部培養の途中報告でグ 自己処置をしていた。受診4週間前より徐々に活動性 ラム陽性球菌は極少量で，グラム陰性桿菌が多数を占 が低下，受診前日より会話困難，受診日当日に歩行不 めていたことから，バンコマイシンの投与を中止しメ 能となり近医を受診した。両下腿に広範な壊疽を認め， ロペネム単剤で治療を継続した。創部を含めた身体所 ＊1埼玉医科大学総合医療センター高度救命救急センター，＊2同 中央検査部 受付日2016年 5 月 2 日 （〒350-8550 埼玉県川越市鴨田1981） 採択日2016年10月 6 日 －337－ 日集中医誌 J Jpn Soc Intensive Care Med Vol. 24 No. 3 Fig. 1 Leg ulcers at time of hospitalization Fig. 2 Colony of Kerstersia gyiorum〔TSAⅡ 5％ Sheep 見が改善し，安定したことから第5病日に紹介元病院 Blood Agar M（日本ベクトン・ディッキンソン）〕 へ転院となった。転院後も創部の感染は悪化を認めず， 第33病日にリハビリ病院へ転院となった。 創部培養では，K. gyiorum（Fig. 2, 3），Proteus mirabilis，Enterococcus faecalisが検出された。真菌 や抗酸菌は検出されず，血液培養も陰性であった。 考 察 今回，創部から検出されたK. gyiorumは，Proteo- bacteria門Betaproteobacteria綱Burkholderiales目 Alcaligenaceae科に分類されるグラム陰性桿菌であ り，2003年にCoenyeらにより初めて同定された。本 菌はAlcaligenes faecalisと生化学的性状が近いとされ ているが，Alcaligenaceae科の他の菌と異なり，オキ シダーゼが陰性で，果実臭を産生しないことが特徴で Fig. 3 Gram-negative coccobacilli ある 1) 。 これまで慢性中耳炎（5例）2)〜5)，下肢の創（3例，う ち菌血症合併1例）4),6),7)，気管支肺胞洗浄液（1例）8)， 例 4),5)，中等度耐性1例 8)と，アズトレオナム，スルファ 尿路（1例）9)から分離された10例の症例が報告された メトキサゾール・トリメトプリム耐性，レボフロキサ のみである。常在菌の可能性も指摘されており，慢性 シン，チカルシリン・クラブラン酸中等度耐性1例5) 炎症部位からの報告が多いが，重症化し敗血症の報告 を認めた。アミノグリコシド系，カルバペネム系への も存在する。 耐性は認めなかった。いずれの報告においても耐性化 このような知見の蓄積が少ない細菌において問題と の機序は検討されていない。本症例もCLSIのother なるのが治療法である。 non-enterobacteriaceaeの判定基準を用いて薬剤感受 K. gyiorumの治療に関して，米国臨床検査標準化 性を検討し，アズトレオナム耐性，シプロフロキサシ 協会（Clinical and Laboratory Standards Institute, ン中等度耐性を認めた（Table 1）。 CLSI）には本菌単独の感受性判定基準が存在せず，過 多剤に耐性報告があることから，感受性判明までは 去の報告ではCLSIのother non-enterobacteriaceaeの 広域抗菌薬の使用も検討される。本症例はメロペネム 基準を用いて8症例で感受性が検討されてい で治療が可能であったが，今後薬剤感受性や治療経験 る 2)〜6),8) 。そのうち菌血症の1例では，セフタジジム， の蓄積が求められる。 セフェピム，タゾバクタム・ピペラシリン耐性，セフォ しかし，現時点での報告例は上述したように多くな タキシム中等度耐性を認めた 6) 。また，その他の症例 い。報告例が少ない原因として，K. gyiorumが細菌 では，シプロフロキサシンに対して7症例中耐性3 学上独立して分類された時期が新しく，確実な同定に －338－ Kerstersia gyiorumによる下肢感染症の1例 Table 1 Antimicrobial susceptibility of the Kerstersia gyiorum Antimicrobial agent MIC（μg/ml） Interpretation AZT ＞16 Resistant PIPC 16 Susceptible TAZ/PIPC ＜8 Susceptible IPM/CS ＜1 Susceptible MEPM ＜1 Susceptible CTX ＜1 Susceptible CTRX ＜1 Susceptible CAZ ＜4 Susceptible CZOP ＜4 Susceptible CFPM ＜2 Susceptible AMK ＜4 Susceptible GM ＜2 Susceptible TOB ＜4 Susceptible MINO ＜2 Susceptible LVFX ＜0.5 Susceptible CPFX 2 Intermediate STFX ＜1 Susceptible ST ＜2 Susceptible Interpretations were assessed in accordance with CLSI criteria for other non-enterobacteriaceae. AMK, amikacin; AZT, aztreonam; CAZ, ceftazidime; CFPM, cefepime; CLSI, Clinical and Laboratory Standards Institute; CPFX, ciprofloxacin; CS, cilastatin; CTRX, ceftriaxone; CTX, cefotaxime; CZOP, cefozopran; GM, gentamicin; IPM, imipenem; LVFX, levofloxacin; MEPM, meropenem; MIC, minimum inhibitory con- centration; MINO, minocycline; PIPC, piperacillin; ST, sulfamethoxazole-trime- thoprim; STFX, sitafloxacin hydrate; TAZ, tazobactam; TOB, tobramycin. は16SリボソームRNA（16S ribosomal RNA, 16S 分類学上16S rRNA塩基配列に基づく系統解析の登 rRNA）の解析や，マトリックス支援レーザー脱離イ 場は画期的で，K. gyiorumに限らず，近年本法に基づ オン化飛行時間型質量分析法（matrix assisted laser く系統解析により，原核生物の分類は大きく変貌し， desorption/ionization time of flight mass 新たな種も多数分類された。本症例は，通常臨床現場 spectrometry, MALDI-TOF MS）による分析が必要で の医師があまり意識しない細菌の分類学の進歩と，細 あり，多くの施設においては実施が困難なことが挙げ 菌検査室の検査機器の進歩により，これまで聞き慣れ られる。他方，当院では，2014年10月より菌種の同 ない起因菌による感染症が増える可能性と，使用する 定方法において，従来の生化学的性状検査に加え 抗菌薬選択の困難さを身をもって体験した貴重な例で MALDI-TOF MSによる分析が追加された。 もあった。今後は検査機器の普及により多施設で MALDI-TOF MS導入後の1年間で，当院では本症例 K. gyiorumの検出が増えることが予想され，本菌に を含めて2例の検体からK. gyiorumが検出された。 対する抗菌薬の感受性および治療経験の蓄積とそれら 導入以前はK. gyiorumの報告例がなく，同定不能ま を啓蒙していくことが望まれる。 たはブドウ糖非発酵グラム陰性桿菌（Pseudomonas まとめ oryzihabitans，Burkholderia cepacia，Acinetobacter spp. など）と報告されていた可能性が高い。 下肢の慢性皮膚損傷部からK. gyiorumが検出され 他施設では，MALDI-TOF MSで同定した菌につい た1例を経験した。時には敗血症を来し，耐性の報告 て，さらに16S rRNA解析を行い，本菌と確定して報 もあることから，さらなる症例の蓄積と研究が進むこ 告しているが4),5),8),9)，今回の症例ではMALDI-TOF とで，より的確な治療方法の確立が期待される。 MSでの同定結果がscore 2.241と高い精度であり，生 化学的性状も矛盾がないことから，これ以上の解析は 行わなかった。 －339－ 日集中医誌 J Jpn Soc Intensive Care Med Vol. 24 No. 3 Kerstersia gyiorum in Tanzania: is it an underappreciated 謝 辞 pathogen in chronic otitis media?. Int J Infect Dis 2014;29: 251-3. K. gyiorumおよび細菌の同定方法についてご教示いただ 4) Pence MA, Sharon J, McElvania Tekippe E, et al. Two いた，当院臨床検査部三橋知明教授に感謝いたします。 cases of Kerstersia gyiorum isolated from sites of chronic infection. J Clin Microbiol 2013;51:2001-4. 本稿の全ての著者には規定されたCOIはない。 5) Uysal EB, Çelik C, Tuzcu N, et al. A case of chronic suppurative otitis media caused by Kerstersia gyiorum. APMIS 2015;123:986-9. 文 献 6) Bostwick AD, Zhang C, Manninen K, et al. Bacteremia caused by Kerstersia gyiorum. J Clin Microbiol 2015;53: 1) Coenye T, Vancanneyt M, Cnockaert MC, et al. 1965-7. Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes 7) Greninger AL, Kozyreva V, Truong CL, et al. Draft faecalis-like organism isolated from human clinical genome sequence of Kerstersia gyiorum CG1, isolated samples, and reclassification of Alcaligenes denitrificans from a leg ulcer. Genome Announc 2015;3:e01036-15. Rüger and Tan 1983 as Achromobacter denitrificans 8) Deutscher M, Severing J, Balada-Llasat JM. Kerstersia comb. nov. Int J Syst Evol Microbiol 2003;53:1825-31. gyiorum isolated from a bronchoalveolar lavage in a 2) Almuzara MN, Barberis CM, Traglia GM, et al. Isolation patient with a chronic tracheostomy. Case Rep Infect Dis of Kerstersia gyiorum from a patient with cholestea- 2014;2014:479581. tomatous chronic otitis media. J Clin Microbiol 9) Ogawa Y, Lee ST, Kasahara K, et al. A first case of 2012;50:3809-11. isolation of Kerstersia gyiorum from urinary tract. J 3) Mwalutende A, Mshana SE, Mirambo MM, et al. Two Infect Chemother 2016;22:265-7. cases of chronic suppurative otitis media caused by Abstract A case of Kerstersia gyiorum detected in a chronic skin lesion on the lower extremity Jun Sugiura＊1, Takashi Mato＊1, Susumu Sekine＊2, Fumihito Arima＊1, Hidenori Oi＊1, Atsushi Yamaguchi＊1, Kazuyuki Nakata＊1, Satoru Sugiyama＊1 ＊1 Department of Emergency and Critical Care Medicine, ＊2 Department of Laboratory Medicine, Saitama Medical Center, Saitama Medical University 1981 Kamoda, Kawagoe, Saitama 350-8550, Japan The patient was a 70-year-old man who was transferred to our hospital by his local physician due to severe sepsis arising from a chronic skin lesion on the lower extremity. Sepsis resolved following aggressive wound treatment and administration of antibiotics. Kerstersia gyiorum (K. gyiorum) was later detected from wound cultures. Progress in the classification and identification of bacteria by 16S rRNA(16S ribosomal RNA) analysis led to the classification of K. gyiorum as a bacterium in 2003, but the number of reported cases remains limited. The difficulty of identifying K. gyiorum is cited as one reason for this lack of reports. In the present case, the bacterium was identified by MALDI-TOF MS (matrix assisted laser desorption/ionization time of flight mass spectrometry). Now that analysis is possible at medical facilities due to advances in testing equipment, bacteria that were previously difficult to identify can be relatively easily identified. An increase in the number of cases of identified bacteria that were previously reported only infrequently and that we were previously unaccustomed to hearing about, such as in the present case, is easily conceivable going forward. Further accumulation of cases and knowledge of treatment are anticipated. Key words: ①MALDI-TOF MS (matrix assisted laser desorption/ionization time of flight mass spectrometry), ②16S rRNA (16S ribosomal RNA) J Jpn Soc Intensive Care Med 2017;24:337-40. －340－.
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    Case Report iMedPub Journals Journal of Infectious Diseases and Treatment 2017 http://www.imedpub.com/ Vol.3 No.1:6 ISSN 2472-1093 DOI: 10.21767/2472-1093.100033 Kerstersia Gyiorum causing Chronic Oti Media: Where a Quinolone Does not Work Berta MP Vela, Rossi Nuñez, Juan Manuel García-Lechuz, Ana I López-Calleja, Antonio Rezusta, María José Revillo Clinical Microbiology Department, Hospital General Universitario, Miguel Servet, Zaragoza, Spain Corresponding author: Juan Manuel García-Lechuz, Microbiología, Hospital Miguel Servet, Paseo Isabel La Católica, 1, Zaragoza, Spain, Tel: 34976553790; E-mail: [email protected] Received date: May 10, 2017; Accepted date: May 19, 2017, Published date: May 25, 2017 Copyright: © 2017 García-Lechuz JM. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation: Berta MPV, Rossi N, García-Lechuz JM, López-Calleja AI, Antonio R, et al. Kerstersia Gyiorum causing Chronic Otitis Media: Where a Quinolone Does not Work. J Infec Dis Treat. 2017, 3:1 bone structures of the medium ear and prone to persist and to produce severe sequelae [1]. Abstract Suppurate COM is known as chronic ear discharge through a tympanic drilling, lasting for at least 6 weeks with periods of Chronic otitis media (COM) is an inflammatory disease inactivity [1]. Well-known risk factors for developing a COM which affects the mucosal and bone structures of the are overcrowded living conditions, recurrent respiratory tract medium ear, insidiously, slowly progressive, prone to infections and smoking [2].

  • New Bacterial Species and Changes to Taxonomic Status from 2012 Through 2015 Erik Munson Marquette University, [email protected]

    Marquette University e-Publications@Marquette Clinical Lab Sciences Faculty Research and Clinical Lab Sciences, Department of Publications 1-1-2017 What's in a Name? New Bacterial Species and Changes to Taxonomic Status from 2012 through 2015 Erik Munson Marquette University, [email protected] Karen C. Carroll Johns Hopkins University Published version. Journal of Clinical Microbiology, Vol. 56, No. 1 (January 2017): 24-42. DOI. © 2017 American Society for Microbiology. Used with permission. MINIREVIEW crossm What’s in a Name? New Bacterial Species and Changes to Taxonomic Status from Downloaded from 2012 through 2015 Erik Munson,a Karen C. Carrollb College of Health Sciences, Marquette University, Milwaukee, Wisconsin, USAa; Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USAb ABSTRACT Technological advancements in fields such as molecular genetics and http://jcm.asm.org/ the human microbiome have resulted in an unprecedented recognition of new bac- Accepted manuscript posted online 19 terial genus/species designations by the International Journal of Systematic and Evo- October 2016 Citation Munson E, Carroll KC. 2017. What's in lutionary Microbiology. Knowledge of designations involving clinically significant bac- a name? New bacterial species and changes to terial species would benefit clinical microbiologists in the context of emerging taxonomic status from 2012 through 2015. pathogens, performance of accurate organism identification, and antimicrobial sus- J Clin Microbiol 55:24–42. https://doi.org/ 10.1128/JCM.01379-16. ceptibility testing. In anticipation of subsequent taxonomic changes being compiled Editor Colleen Suzanne Kraft, Emory University by the Journal of Clinical Microbiology on a biannual basis, this compendium summa- Copyright © 2016 American Society for on January 23, 2017 by Marquette University Libraries rizes novel species and taxonomic revisions specific to bacteria derived from human Microbiology.

  • Development of a PCR-Based Method for Monitoring the Status of Alcaligenes Species in the Agricultural Environment

    Biocontrol Science, 2014, Vol. 19, No. 1, 23－31 Original Development of a PCR-Based Method for Monitoring the Status of Alcaligenes Species in the Agricultural Environment MIYO NAKANO1, MASUMI NIWA2, AND NORIHIRO NISHIMURA1＊ 1 Department of Translational Medical Science and Molecular and Cellular Pharmacology, Pharmacogenomics, and Pharmacoinformatics, Mie University Graduate School of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan 2 DESIGNER FOODS. Co., Ltd. NALIC207, Chikusa 2-22-8, Chikusa-ku, Nagoya, Aichi 464-0858, Japan Received 1 April, 2013/Accepted 14 September, 2013 To analyze the status of the genus Alcaligenes in the agricultural environment, we developed a PCR method for detection of these species from vegetables and farming soil. The selected PCR primers amplified a 107-bp fragment of the 16S rRNA gene in a specific PCR assay with a detection limit of 1.06 pg of pure culture DNA, corresponding to DNA extracted from approxi- mately 23 cells of Alcaligenes faecalis. Meanwhile, PCR primers generated a detectable amount of the amplicon from 2.2×102 CFU/ml cell suspensions from the soil. Analysis of vegetable phyl- loepiphytic and farming soil microbes showed that bacterial species belonging to the genus Alcaligenes were present in the range from 0.9×100 CFU per gram（ or cm2）（ Japanese radish: Raphanus sativus var. longipinnatus） to more than 1.1×104 CFU/g（ broccoli flowers: Brassica oleracea var. italic）, while 2.4×102 to 4.4×103 CFU/g were detected from all soil samples. These results indicated that Alcaligenes species are present in the phytosphere at levels 10–1000 times lower than those in soil.
  • Chronic Lower Extremity Wound Infection

    Chronic Lower Extremity Wound Infection

    Baran et al. BMC Infectious Diseases (2017) 17:608 DOI 10.1186/s12879-017-2711-3 CASEREPORT Open Access Chronic lower extremity wound infection due to Kerstersia gyiorum in a patient with Buerger’s disease: a case report Irmak Baran1,3* , Arife Polat Düzgün2, İpek Mumcuoğlu1 and Neriman Aksu1 Abstract Background: Kerstersia gyiorum is an extremely rare pathogen of human infection. It can cause chronic infection in patients with underlying conditions. It can easily be misdiagnosed if proper diagnostic methods are not used. Case presentation: A 47-year-old male patient with a history of Buerger’s Disease for 28 years presented to our hospital with an infected chronic wound on foot. The wound was debrided, and the specimen was sent to Microbiology laboratory. Gram staining of the specimen showed abundant polymorphonuclear leukocytes and gram-negative bacilli. Four types of colonies were isolated on blood agar. These were identified as Kerstersia gyiorum, Proteus vulgaris, Enterobacter cloacae, Morganella morganii by Maldi Biotyper (Bruker Daltonics, Germany). The identification of K. gyiorum was confirmed by 16S ribosomal RNA gene sequencing. The patient was successfully recovered with antimicrobial therapy, surgical debridement, and skin grafting. Conclusions: This is the first case of wound infection due to K. gyiorum in a patient with Buerger’sDisease. We made a brief review of K. gyiorum cases up to date. Also, this case is presented to draw attention to the use of new and advanced methods like MALDI-TOF MS and 16S rRNA gene sequencing for identification of rarely isolated species from clinical specimens of patients with chronic infections and with chronic underlying conditions.
  • Genomic Characterization of Kerstersia Gyiorum SWMUKG01, an Isolate from a Patient with Respiratory Infection in China

    Genomic Characterization of Kerstersia Gyiorum SWMUKG01, an Isolate from a Patient with Respiratory Infection in China

    RESEARCH ARTICLE Genomic characterization of Kerstersia gyiorum SWMUKG01, an isolate from a patient with respiratory infection in China Ying Li1☯, Min Tang2☯, Guangxi Wang2, Chengwen Li1, Wenbi Chen2, Yonghong Luo3, 2 2 1 1 2 Jing Zeng , Xiaoyan Hu , Yungang Zhou , Yan Gao , Luhua ZhangID * 1 Department of Immunology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, Sichuan, China, 2 Department of Pathogenic Biology, School of Basic Medical Sciences, Southwest Medical University, Luzhou, Sichuan, China, 3 Department of Physiology, School of Basic Medical Sciences, a1111111111 Southwest Medical University, Luzhou, Sichuan, China a1111111111 a1111111111 ☯ These authors contributed equally to this work. a1111111111 * [email protected] a1111111111 Abstract OPEN ACCESS Background Citation: Li Y, Tang M, Wang G, Li C, Chen W, Luo Y, et al. (2019) Genomic characterization of The Gram-negative bacterium Kerstersia gyiorum, a potential etiological agent of clinical Kerstersia gyiorum SWMUKG01, an isolate from a infections, was isolated from several human patients presenting clinical symptoms. Its sig- patient with respiratory infection in China. PLoS nificance as a possible pathogen has been previously overlooked as no disease has thus far ONE 14(4): e0214686. https://doi.org/10.1371/ journal.pone.0214686 been definitively associated with this bacterium. To better understand how the organism contributes to the infectious disease, we determined the complete genomic sequence of K. Editor: Yung-Fu Chang, Cornell University, UNITED STATES gyiorum SWMUKG01, the first clinical isolate from southwest China. Received: February 13, 2019 Results Accepted: March 18, 2019 The genomic data obtained displayed a single circular chromosome of 3, 945, 801 base Published: April 12, 2019 pairs in length, which contains 3, 441 protein-coding genes, 55 tRNA genes and 9 rRNA Copyright: © 2019 Li et al.

  • Antibacterial Potential of Heterotrophic Bacteria Isolated in Siak River Estuary, Indonesia, Against Pathogens in Fish

    Antibacterial potential of heterotrophic bacteria isolated in Siak River estuary, Indonesia, against pathogens in fish 1Feli Feliatra, 1Nursyirwani Nursyirwani, 1Andrei P. Zirma, 2Iesje Lukistyowati, 3Aras Mulyadi, 2Adelina Adelina 1 Laboratory of Marine Microbiology, Faculty of Fisheries and Marine Sciences, University of Riau, Riau Province, Indonesia; 2 Laboratory of Parasite and Fish Diseases, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, University of Riau, Riau Province, Indonesia; 3 Laboratory of Biology, Faculty of Fisheries and Marine Sciences, University of Riau, Riau Province, Indonesia. Corresponding author: F. Feliatra, [email protected] Abstract. One of the major challenges of aquaculture is fish disease. However, heterotrophic bacteria are natural antibiotics capable of solving the problem. Heterotrophic bacteria are abundant worldwide, and use organic materials as a nutritional source. The aims of this research were to isolate and to identify heterotrophic bacteria from Siak River at water salinities of 5 ppt and 25 ppt. Selected bacterial isolates were examined for their ability to inhibit the growth of pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas sp.). Out of the 25 heterotrophic bacterial strains selected with the ability to act as anti-pathogenic bacteria in aquatic animals, only 8 have great potential to inhibit the growth of the three pathogenic bacteria. Through identification of heterotrophic bacteria with 16s rDNA technique and BLAST system search, it was discovered that three isolates (A11, A22, A23) were similar to Kerstersia gyiorum and one isolate (A14) was similar to Enterococcus sp. Four other isolates were not identified considering the fact that the species have not been registered in GenBank or there was a great possibility that they have not been identified prior to this study.
  • Evidence for Loss and Reacquisition of Alcoholic Fermentation in A

    Evidence for Loss and Reacquisition of Alcoholic Fermentation in A

    RESEARCH ARTICLE Evidence for loss and reacquisition of alcoholic fermentation in a fructophilic yeast lineage Carla Gonc¸alves1, Jennifer H Wisecaver2,3, Jacek Kominek4,5,6,7, Madalena Salema Oom1,8, Maria Jose´ Leandro9,10, Xing-Xing Shen2, Dana A Opulente4,5,6,7, Xiaofan Zhou11,12, David Peris4,5,6,7,13, Cletus P Kurtzman14†, Chris Todd Hittinger4,5,6,7, Antonis Rokas2, Paula Gonc¸alves1* 1UCIBIO-REQUIMTE, Departamento de Cieˆncias da Vida, Faculdade de Cieˆncias e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal; 2Department of Biological Sciences, Vanderbilt University, Nashville, United States; 3Department of Biochemistry, Purdue Center for Plant Biology, Purdue University, West Lafayette, United States; 4Laboratory of Genetics, University of Wisconsin-Madison, Madison, United States; 5DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, United States; 6J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, Madison, United States; 7Wisconsin Energy Institute, University of Wisconsin-Madison, Madison, United States; 8Centro de Investigac¸a˜ o Interdisciplinar Egas Moniz, Instituto Universita´rio Egas Moniz, Caparica, Portugal; 9Instituto de Tecnologia Quı´mica e Biolo´gica Anto´nio Xavier, Universidade Nova de Lisboa, Av. da Repu´ blica, Oeiras, Portugal; 10LNEG – Laborato´rio Nacional de Energia e Geologia, Unidade de Bioenergia (UB), Lisboa, Portugal; 11Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China; 12Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China; *For correspondence: 13Department of Food Biotechnology, Institute of Agrochemistry and Food [email protected] Technology (IATA), CSIC, Valencia, Spain; 14Mycotoxin Prevention and Applied †Deceased Microbiology Research Unit, National Center for Agricultural Utilization Research, Competing interests: The Agricultural Research Service, U.S.
  • Two Cases of Kerstersia Gyiorum Isolated from Sites of Chronic Infection Morgan A

    Two Cases of Kerstersia Gyiorum Isolated from Sites of Chronic Infection Morgan A

    Washington University School of Medicine Digital Commons@Becker Open Access Publications 2013 Two cases of Kerstersia gyiorum isolated from sites of chronic infection Morgan A. Pence Washington University School of Medicine in St. Louis Jeffrey Sharon Washington University School of Medicine in St. Louis Erin McElvania Tekippe Washington University School of Medicine in St. Louis Brittany L. Pakalniskis University of Iowa Bradley A. Ford University of Iowa See next page for additional authors Follow this and additional works at: https://digitalcommons.wustl.edu/open_access_pubs Recommended Citation Pence, Morgan A.; Sharon, Jeffrey; McElvania Tekippe, Erin; Pakalniskis, Brittany L.; Ford, Bradley A.; and Burnham, Carey-Ann D., ,"Two cases of Kerstersia gyiorum isolated from sites of chronic infection." Journal of Clinical Microbiology.51,6. 2001-2004. (2013). https://digitalcommons.wustl.edu/open_access_pubs/2332 This Open Access Publication is brought to you for free and open access by Digital Commons@Becker. It has been accepted for inclusion in Open Access Publications by an authorized administrator of Digital Commons@Becker. For more information, please contact [email protected]. Authors Morgan A. Pence, Jeffrey Sharon, Erin McElvania Tekippe, Brittany L. Pakalniskis, Bradley A. Ford, and Carey-Ann D. Burnham This open access publication is available at Digital Commons@Becker: https://digitalcommons.wustl.edu/open_access_pubs/2332 Two Cases of Kerstersia gyiorum Isolated from Sites of Chronic Infection Morgan A. Pence, Jeffrey Sharon, Erin McElvania Tekippe, Brittany L. Pakalniskis, Bradley A. Ford and Carey-Ann D. Burnham J. Clin. Microbiol. 2013, 51(6):2001. DOI: Downloaded from 10.1128/JCM.00829-13. Published Ahead of Print 17 April 2013.