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US009765324B2 (12 ) United States Patent ( 10 ) Patent No. : US 9 ,765 , 324 B2 Corgie et al. (45 ) Date of Patent: Sep. 19, 2017 (54 ) HIERARCHICAL MAGNETIC ( 2013 .01 ) ; C12N 11/ 14 ( 2013 .01 ); C12N 13/ 00 NANOPARTICLE ENZYME MESOPOROUS ( 2013 .01 ) ; C12P 7 /04 ( 2013 . 01 ) ; C12P 7 / 22 ASSEMBLIES EMBEDDED IN ( 2013 . 01 ) ; C12P 17 /02 ( 2013 .01 ) ; B01J MACROPOROUS SCAFFOLDS 35 / 1061 ( 2013 .01 ) ; B01J 35 / 1066 (2013 .01 ) ; BOIJ 2208 /00805 ( 2013 . 01 ) ; B01J 2219 /0854 (71 ) Applicant: CORNELL UNIVERSITY , Ithaca , NY ( 2013 .01 ) ; BOIJ 2219 /0862 ( 2013 .01 ) ; CO2F (US ) 2101/ 327 (2013 .01 ) ; CO2F 2209 / 006 (2013 . 01 ) ; CO2F 2303 /04 (2013 .01 ) ; (72 ) Inventors : Stephane C . Corgie , Ithaca , NY (US ) ; Xiaonan Duan , Ithaca, NY (US ) ; ( Continued ) Emmanuel Giannelis , Ithaca , NY (US ) ; (58 ) Field of Classification Search Daniel Aneshansley , Ithaca , NY (US ) ; None Larry P . Walker , Ithaca , NY (US ) See application file for complete search history . (73 ) Assignee : CORNELL UNIVERSITY , Ithaca , NY (56 ) References Cited (US ) U . S . PATENT DOCUMENTS ( * ) Notice : Subject to any disclaimer, the term of this 4 , 152, 210 A 5 / 1979 Robinson et al. patent is extended or adjusted under 35 6 , 440 , 711 B18 / 2002 Dave U . S . C . 154 ( b ) by 73 days. (Continued ) ( 21) Appl. No .: 14 /433 , 242 FOREIGN PATENT DOCUMENTS CN 1580233 A * 2 / 2005 ( 22 ) PCT Filed : Oct. 4 , 2013 CN 101109016 A 1 / 2008 ( 86 ) PCT No .: PCT/ US2013 / 063441 (Continued ) $ 371 ( c ) ( 1 ), OTHER PUBLICATIONS ( 2 ) Date : Apr. 2 , 2015 Corgie S . et al . Self Assembled Complexes of Horseradish (87 ) PCT Pub . No. : WO2014 / 055853 Peroxidase with Magnetic Nanoparticles Showing Enhanced Peroxidase Activity . Advanced Functional Materials vol. 22, 1940 PCT Pub . Date : Apr. 10 , 2014 1951, Feb . 15 , 2012 .* (65 ) Prior Publication Data (Continued ) US 2015 /0252352 A1 Sep . 10 , 2015 Primary Examiner — Ralph Gitomer (74 ) Attorney , Agent, or Firm — Scully Scott Murphy & Related U . S . Application Data Presser (60 ) Provisional application No . 61 /710 , 110 , filed on Oct . 5 , 2012 , provisional application No. 61/ 767 ,477 , filed ( 57 ) ABSTRACT on Feb . 21 , 2013 . A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated (51 ) Int . Cl. mesoporous aggregates of magnetic nanoparticles , wherein B01J 31 /00 ( 2006 . 01 ) an enzyme is embedded in mesopores of the mesoporous C12N 11 /04 ( 2006 .01 ) aggregates of magnetic nanoparticles . Methods for synthe A62D 3 / 02 ( 2007 . 01 ) sizing the hierarchical catalyst composition are also B01J 19 / 12 ( 2006 .01 ) described . Also described are processes that use the recov B01J 31 /02 ( 2006 .01 ) erable hierarchical catalyst composition for depolymerizing (Continued ) lignin , remediation of water contaminated with aromatic (52 ) U . S . CI. substances, polymerizing monomers by a free -radical ??? .. .. C12N 11 /04 ( 2013 .01 ) ; A62D 3 / 02 mechanism , epoxidation of alkenes, halogenation of phe (2013 .01 ) ; B01J 8 / 00 ( 2013 .01 ) ; B01J 19 / 12 nols , inhibiting growth and function of microorganisms in a ( 2013 .01 ) ; B01J 31 /003 ( 2013 . 01 ) ; B01J solution , and carbon dioxide conversion to methanol. Fur 31 /02 ( 2013 .01 ) ; B01J 35 /0033 (2013 .01 ) ; ther described are methods for increasing the space time B01J 35 / 04 ( 2013 . 01 ) ; B01J 37 / 02 ( 2013 . 01 ) ; yield and / or total turnover number of a liquid - phase chemi CO2F 1 /484 ( 2013 .01 ) ; C02F 3 / 00 ( 2013 .01 ) ; cal reaction that includes magnetic particles to facilitate the CO2F 3 /342 ( 2013 . 01 ) ; C07C 1 / 12 (2013 . 01) ; chemical reaction , the method comprising subjecting the CO7D 301 /22 (2013 .01 ) ; C07G 1/ 00 chemical reaction to a plurality ofmagnetic fields of selected ( 2013. 01 ) ; C08F 14 / 00 ( 2013 . 01 ) ; C08F 18/ 00 magnetic strength , relative position in the chemical reaction , ( 2013 .01 ) ; C12N 9 / 0004 ( 2013 .01 ) ; C12N and relative motion . 970006 ( 2013 .01 ) ; C12N 9 /0061 (2013 .01 ) ; C12N 9 / 0065 (2013 .01 ) ; C12N 11/ 08 25 Claims, 22 Drawing Sheets US 9 ,765 , 324 B2 Page 2 (51 ) Int . Cl. Abdullah M . et al. , “ Preparation of Oxide Particles With Ordered B01J 35 / 04 ( 2006 .01 ) Macropores by Colloidal Templating and Spray Pyrolysis ” , Acta Materialia 52 : 5151 - 5156 ( 2004 ) . B01J 37 /02 ( 2006 .01 ) Azevedo A . M . et al. , “ Horseradish Peroxidase : A Valuable Tool in CO2F 1 / 48 ( 2006 .01 ) Biotechnology ” , Biotechnology Annual Review 9 : 199 - 247 ( 2003 ) . CO2F 3 /00 ( 2006 . 01 ) Chalkias N . G . et al . , “ Activity Increase of Horseradish Peroxidase C02F 3 /34 ( 2006 .01 ) in the Presence ofMagnetic Particles” , J . Am . Chem . Soc . 130 :2910 C07C 1 / 12 ( 2006 .01 ) 2911 ( 2008 ) . C07G 1 /00 ( 2011. 01 ) Corgie S . C . et al. , “ Self - Assembled Complexes of Horseradish C08F 14/ 00 ( 2006 .01 ) Peroxidase with Magnetic Nanoparticles Showing Enhanced C08F 18 / 00 ( 2006 . 01 ) Peroxidase Activity ” , Advanced Functional Materials 22 : 1940 1951 ( Feb . 15 , 2012 ) . C07D 301 / 22 ( 2006 .01 ) Corvini P . F . X . et al ., “ Lance : Laccase -Nanoparticle Conjugates for C12N 11 / 14 ( 2006 . 01 ) the Elimination of Micropollutants ( Endocrine Disrupting Chemi C12P 7 / 22 ( 2006 . 01 ) cals ) from Wastewater in Bioreactors ” , Rev Environ Sci Biotechnol C12N 9 / 02 ( 2006 . 01) 9 : 23 - 27 ( 2010 ) . C12N 9 /04 ( 2006 . 01 ) Davis M . et al ., “ Formation of Three - Dimensional Ordered Hier C12N 9 / 08 ( 2006 .01 ) archically Porous Metal Oxides Via a Hybridized Epoxide Assisted / C12N 13 / 00 ( 2006 . 01) Colloidal Crystal Templating Approach ” , ACS Applied Materials & C12P 7 / 04 ( 2006 . 01 ) Interfaces 5 :7786 -7792 (2013 ) . C12P 17 /02 ( 2006 .01 ) Huang J. et al. , “ Zinc Tetraaminophthalocyanine -Fe304 Nanoparticle Composite for Laccase Immobilization ” , Interna B01J 35 / 00 ( 2006 . 01 ) tional Journal of Nanomedicine 2 ( 4 ) :775 -784 ( 2007 ) . B01J 8 / 00 ( 2006 .01 ) Luo X - L et al. , “ Electrochemically Deposited Chitosan Hydrogel CI2N 11 /08 ( 2006 .01 ) for Horseradish Peroxidase Immobilization Through Gold B01J 35 / 10 (2006 .01 ) Nanoparticles Self - Assembly " , Biosensors and Bioelectronics CO2F 101/ 32 ( 2006 .01 ) 21: 190 - 196 ( 2005 ) . (52 ) U .S . CI. Morrison M . et al. , “ Peroxidase - Catalyzed Halogenation ” , Annual CPC .. C02F 2305 /08 ( 2013 .01 ) ; C12Y 101/ 03004 Review of Biochemistry 45 : 861 -888 ( Jul . 1976 ) , abstract. Niu T . et al . , “ Preparation of Meso -Macroporous a — Alumina ( 2013 .01 ) ; C12Y 111/ 01 (2013 . 01 ) ; YO2P Using Carbon Nanotube as the Template for the Mesopore and Their 20 / 588 ( 2015 . 11) Application to the Preferential Oxidation of CO in HZ- Rich Gases” , J Porous Mater 20 : 789 - 798 ( 2013 ) . (56 ) References Cited Seelan S . et al. , “ Macroporous Ceramics Coated With Mesoporous Layer for Enzyme Encapsulation ” , Key Engineering Materials U . S . PATENT DOCUMENTS 317 - 318 :717 - 722 ( 2006 ) . 7 ,459 , 145 B2 12 / 2008 Bao et al . Tang D . et al ., “ Direct Electrochemical Immunoassay Based on 7 , 485 , 367 B2 2 / 2009 Chen et al. Immobilization of Protein -Magnetic Nanoparticle Composites on to 9 ,597 ,672 B2 * 3 / 2017 Corgie . B82Y 30 / 00 Magnetic Electrode Surfaces by Sterically Enhanced Magnetic 2006 /0289354 Al 12 / 2006 Zhou et al. Field Force” , Biotechnology Letters 28 :559 -565 (2006 ) . 2008 / 0305048 A1 12 / 2008 Josephson et al. Veitch N . C . , “ Horseradish Peroxidase: A Modern View of a Classic 2010 / 0056360 A1 3 / 2010 Lee Enzyme” , Phytochemistry 65 : 249 - 259 ( 2004 ) . 2010 / 0056816 A1 3 / 2010 Wallin et al. Wang F . et al. , “ Magnetic Mesoporous Silica Nanoparticles : Fab 2014 / 0004583 A1 * 1/ 2014 Corgie . .. .. .. B82Y 30 / 00 rication and Their Laccase Immobilization Performance ” , 435 / 156 Bioresource Technology 101: 8931 -8935 ( 2010 ) . Yang H - H et al ., “ Magnetite - Containing Spherical Silica FOREIGN PATENT DOCUMENTS Nanoparticles for Biocatalysis and Bioseparations” , Analytical Chemistry 76 ( 5 ) : 1316 - 1321 (Mar . 1 , 2004 ) . CN 101198255 A 6 /2008 Yang L . et al. , “ Robust MacroporousMaterials of Chiral Polyaniline KR 2011 /033575 A * 3 / 2011 Composites” , Chem . Mater . 18 ( 2 ) :297 - 300 (2006 ) . SU 1000098 A 2 / 1983 International Search Report dated Oct . 10 , 2012 received from the WO WO 2012 / 122437 A2 9 / 2012 Korean Intellectual Property Office from Application No. PCT/ US2012 /028392 . OTHER PUBLICATIONS International Search Report dated Feb . 20 , 2014 received from Application No . PCT/ US2013 / 063441. Wang , F . et al. Magnetic Mesoporous Silica Nanoparticles . Ping , Z . et al ., “ Research and application of magnetic fluidized Bioresource Technology vol. 101 8931 - 5 , 2010 . * bed ” , Chemical Industry and Engineering Progress , ( Apr. 25 , 2006 ) , Kim J . et al. A Magnetically Separable Highly Stable Enzyme pp . 371 - 377 , with English abstract. System Based on Nanocomposites of Enzymes . Small 1 ( 12 ) Chinese Office Action dated Mar . 23 , 2016 issued in corresponding 1203 - 7 , 2005. * Chinese Patent Application No . 201380063389 . 8 with English Kim , M . et al. Colorimetric Quantification of Galactose Using a language translation . Nanostructured Multicatalyst System . Analyst 137 ( 5 ) 1137 - 1143 , Supplementary Partial European Search Report dated Apr. 29 , 2016 2012 . * issued in EP 13844083 . 9 . Lee J . et al . Magnetically Separable and Higly Stable Enzyme Supplementary European Search Report dated Sep
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    ΔΘΝΗΚΟ ΚΑΗ ΚΑΠΟΓΗ΢ΣΡΗΑΚΟ ΠΑΝΔΠΗ΢ΣΖΜΗΟ ΑΘΖΝΩΝ ΢ΥΟΛΖ ΘΔΣΗΚΩΝ ΔΠΗ΢ΣΖΜΩΝ ΣΜΖΜΑ ΥΖΜΔΗΑ΢ ΜΔΣΑΠΣΤΥΗΑΚΟ ΠΡΟΓΡΑΜΜΑ ΢ΠΟΤΓΧΝ «ΚΑΣΑΛΤ΢Ζ ΚΑΗ ΔΦΑΡΜΟΓΔ΢ ΣΖ΢» ΔΡΔΤΝΖΣΗΚΖ ΔΡΓΑ΢ΗΑ ΓΗΠΛΧΜΑΣΟ΢ ΔΗΓΗΚΔΤ΢Ζ΢ Ομεηδναλαγωγηθά έλδπκα ωο πξόηππα εθαξκνγήο ηεο κεζόδνπ QSAR (Quantitative Structure-Activity relations) ΠΑΝΑΓΗΧΣΑ ΢. ΣΕΑΝΖ ΜΖΥΑΝΗΚΟ΢ ΣΔΥΝΟΛΟΓΗΑ΢ ΠΔΣΡΔΛΑΗΟΤ ΚΑΗ ΦΤ΢ΗΚΟΤ ΑΔΡΗΟΤ ΑΘΖΝΑ ΝΟΔΜΒΡΗΟ΢ 2011 ΔΡΔΤΝΖΣΗΚΖ ΔΡΓΑ΢ΗΑ ΓΗΠΛΧΜΑΣΟ΢ ΔΗΓΗΚΔΤ΢Ζ΢ Ομεηδναλαγσγηθά έλδπκα σο πξφηππα ηεο εθαξκνγήο ηεο κεζφδνπ QSAR (Quantitative Structrure-Activity relations) ΠΑΝΑΓΗΧΣΑ ΢. ΣΕΑΝΖ Α.Μ.: 281604 ΔΠΗΒΛΔΠΧΝ ΚΑΘΖΓΖΣΖ΢: Ι. ΜΑΡΚΟΠΟΤΛΟ΢ , Αλαπιεξσηήο Καζεγεηήο ΔΚΠΑ ΣΡΗΜΔΛΖ΢ ΔΞΔΣΑ΢ΣΗΚΖ ΔΠΗΣΡΟΠΖ Η. ΜΑΡΚΟΠΟΤΛΟ΢ Αλαπιεξωηεο θαζεγεηήο, Σκήκα Υεκείαο, ΔΚΠΑ Γ. ΥΑΣΕΖΝΗΚΟΛΑΟΤ Δπίθνπξνο Καζεγεηήο, Σκήκα Βηνινγίαο, ΔΚΠΑ Π. ΠΑΡΑ΢ΚΔΤΟΠΟΤΛΟΤ Λέθηνξαο, Σκήκα Υεκείαο, ΔΚΠΑ ΗΜΔΡΟΜΗΝΙΑ ΔΞΔΣΑ΢Η΢ 25/11/2011 2 ΠΔΡΗΛΖΦΖ ΢ε απηή ηε δηαηξηβή αξρηθά παξνπζηάδνληαη νη γεληθνί κεραληζκνί ελδπκηθήο θαηάιπζεο νη νπνίνη ζηε ζπλέρεηα εμεηδηθεχνληαη ζηνπο αληίζηνηρνπο κεραληζκνχο ησλ νμεηδνλαγσγηθψλ ζπζηεκάησλ ησλ θαηαιαζψλ θαη ππεξνμεηδαζψλ. Σν έλδπκν ππεξνμεηδάζε ηνπ αγξηνξάπαλνπ (horseradish peroxidase–HRP) απνηειεί ην θεληξηθφ έλδπκν αλαθνξάο ησλ αλσηέξσ κεραληζκψλ θαηάιπζεο ζηνπο νπνίνπο βαζίδεηαη θαη ε κειέηε ησλ ζρέζεσλ δνκήο-δξαζηηθφηεηαο ησλ ζπζηεκάησλ απησλ κέζσ ηεο κεζνδνινγίαο QSAR. Η εξγαζία νινθιεξψλεηαη κε ηελ αλάιπζε ησλ πεηξακαηηθψλ δεδνκέλσλ ηεο βηβιηνγξαθίαο πνπ αθνξνχλ ζηελ πξφβιεςε ησλ ηηκψλ θαη ησλ θηλεηηθψλ παξακέηξσλ δξάζεο ηεο HRP κέζσ QSAR ελψ εμάγνληαη ζπκπεξάζκαηα ηα νπνία απνδεηθλχνπλ ηφζν ηελ εθαξκνζηκφηεηα φζν θαη ηε ρξεζηκφηεηα ηεο πξνηεηλφκελεο κεζνδνινγίαο ζηα ζπζηήκαηα καο. ΘΔΜΑΣΗΚΖ ΠΔΡΗΟΥΖ: Δλδπκηθή θαηάιπζε ΛΔΞΔΗ΢ ΚΛΔΗΓΗΑ: βηνθαηάιπζε, έλδπκα, θαηαιάζεο, ππεξνμεηδάζε ηνπ αγξηνξάπαλνπ (HRP), ζρέζεηο δνκήο-δξαζηηθφηεηαο (QSAR) 3 ABSTRACT In this essay we present the concept of enzymatic catalysis and the general methods for the investigation of this phenomenon. We concentrated on the studies of oxidoreductases and especially in catalases and peroxidases.
  • 12) United States Patent (10

    12) United States Patent (10

    US007635572B2 (12) UnitedO States Patent (10) Patent No.: US 7,635,572 B2 Zhou et al. (45) Date of Patent: Dec. 22, 2009 (54) METHODS FOR CONDUCTING ASSAYS FOR 5,506,121 A 4/1996 Skerra et al. ENZYME ACTIVITY ON PROTEIN 5,510,270 A 4/1996 Fodor et al. MICROARRAYS 5,512,492 A 4/1996 Herron et al. 5,516,635 A 5/1996 Ekins et al. (75) Inventors: Fang X. Zhou, New Haven, CT (US); 5,532,128 A 7/1996 Eggers Barry Schweitzer, Cheshire, CT (US) 5,538,897 A 7/1996 Yates, III et al. s s 5,541,070 A 7/1996 Kauvar (73) Assignee: Life Technologies Corporation, .. S.E. al Carlsbad, CA (US) 5,585,069 A 12/1996 Zanzucchi et al. 5,585,639 A 12/1996 Dorsel et al. (*) Notice: Subject to any disclaimer, the term of this 5,593,838 A 1/1997 Zanzucchi et al. patent is extended or adjusted under 35 5,605,662 A 2f1997 Heller et al. U.S.C. 154(b) by 0 days. 5,620,850 A 4/1997 Bamdad et al. 5,624,711 A 4/1997 Sundberg et al. (21) Appl. No.: 10/865,431 5,627,369 A 5/1997 Vestal et al. 5,629,213 A 5/1997 Kornguth et al. (22) Filed: Jun. 9, 2004 (Continued) (65) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2005/O118665 A1 Jun. 2, 2005 EP 596421 10, 1993 EP 0619321 12/1994 (51) Int. Cl. EP O664452 7, 1995 CI2O 1/50 (2006.01) EP O818467 1, 1998 (52) U.S.
  • Mushroom Ligninolytic Enzymes―Features and Application Of

    Mushroom Ligninolytic Enzymes―Features and Application Of

    applied sciences Review Mushroom Ligninolytic Enzymes—Features and Application of Potential Enzymes for Conversion of Lignin into Bio-Based Chemicals and Materials Seonghun Kim 1,2 1 Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup 56212, Korea; [email protected]; Tel.: +82-63-570-5113 2 Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Daejeon 34113, Korea Abstract: Mushroom ligninolytic enzymes are attractive biocatalysts that can degrade lignin through oxido-reduction. Laccase, lignin peroxidase, manganese peroxidase, and versatile peroxidase are the main enzymes that depolymerize highly complex lignin structures containing aromatic or aliphatic moieties and oxidize the subunits of monolignol associated with oxidizing agents. Among these enzymes, mushroom laccases are secreted glycoproteins, belonging to a polyphenol oxidase family, which have a powerful oxidizing capability that catalyzes the modification of lignin using synthetic or natural mediators by radical mechanisms via lignin bond cleavage. The high redox potential laccase within mediators can catalyze the oxidation of a wide range of substrates and the polymeriza- tion of lignin derivatives for value-added chemicals and materials. The chemoenzymatic process using mushroom laccases has been applied effectively for lignin utilization and the degradation of recalcitrant chemicals as an eco-friendly technology. Laccase-mediated grafting