Anti-APEH Antibody (ARG57339)

Product datasheet [email protected]

ARG57339 Package: 100 μl anti-APEH antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes APEH

Tested Reactivity Hu, Ms, Rat

Tested Application ICC/IF, IP, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name APEH

Antigen Species Human

Immunogen Recombinant Protein of Human APEH.

Conjugation Un-conjugated

Alternate Names D3S48E; OPH; EC 3.4.19.1; Oxidized protein hydrolase; D3F15S2; APH; Acylaminoacyl-peptidase; Acylamino-acid-releasing enzyme; DNF15S2; Acyl-peptide hydrolase; ACPH; AARE

Application Instructions

Application table Application Dilution

ICC/IF 1:10 - 1:100

IP Assay-dependent

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control Jurkat

Calculated Mw 81 kDa

Properties

Form Liquid

Purification Affinity purification with immunogen.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/3 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol APEH

Gene Full Name acylaminoacyl-peptide hydrolase

Background This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008]

Function This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. [UniProt]

Images

ARG57339 anti-APEH antibody ICC/IF image

Immunofluorescence: HeLa cells stained with ARG57339 anti-APEH antibody.

ARG57339 anti-APEH antibody WB image

Western blot: Jurkat cell lysate stained with ARG57339 anti-APEH antibody.

www.arigobio.com 2/3 ARG57339 anti-APEH antibody IP image

Immunoprecipitation: 100 µg extracts of SW480 cells were immunoprecipitated and stained with ARG57339 anti-APEH antibody at 1:1000 dilution.