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Anti-APEH Antibody (ARG57339)

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Anti-APEH Antibody (ARG57339) Product datasheet [email protected] ARG57339 Package: 100 μl anti-APEH antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes APEH Tested Reactivity Hu, Ms, Rat Tested Application ICC/IF, IP, WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name APEH Antigen Species Human Immunogen Recombinant Protein of Human APEH. Conjugation Un-conjugated Alternate Names D3S48E; OPH; EC 3.4.19.1; Oxidized protein hydrolase; D3F15S2; APH; Acylaminoacyl-peptidase; Acylamino-acid-releasing enzyme; DNF15S2; Acyl-peptide hydrolase; ACPH; AARE Application Instructions Application table Application Dilution ICC/IF 1:10 - 1:100 IP Assay-dependent WB 1:500 - 1:2000 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Positive Control Jurkat Calculated Mw 81 kDa Properties Form Liquid Purification Affinity purification with immunogen. Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. Preservative 0.02% Sodium azide Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/3 Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol APEH Gene Full Name acylaminoacyl-peptide hydrolase Background This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. [provided by RefSeq, Jul 2008] Function This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. [UniProt] Images ARG57339 anti-APEH antibody ICC/IF image Immunofluorescence: HeLa cells stained with ARG57339 anti-APEH antibody. ARG57339 anti-APEH antibody WB image Western blot: Jurkat cell lysate stained with ARG57339 anti-APEH antibody. www.arigobio.com 2/3 ARG57339 anti-APEH antibody IP image Immunoprecipitation: 100 µg extracts of SW480 cells were immunoprecipitated and stained with ARG57339 anti-APEH antibody at 1:1000 dilution. www.arigobio.com 3/3 Powered by TCPDF (www.tcpdf.org).
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