Monoclonal Antibody to TFE3

AM10141SU-N OriGene Technologies Inc. OriGene EU

Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-858-888-7900 Phone: +49-5221-34606-0 Fax: +1-858-888-7904 Fax: +49-5221-34606-11 [email protected] [email protected]

Monoclonal Antibody to TFE3 - Supernatant Alternate names: Transcription factor E3 Catalog No.: AM10141SU-N Quantity: 1 ml Background: Xp11 translocation renal cell carcinomas (RCC) are a recently recognized subset of RCC, characterized by chromosome translocations involving the Xp11.2 break point and resulting in gene fusions involving the TFE3 transcription factor gene that maps to this locus. Xp11 translocation RCC represents the most common type of RCC in children. The most sensitive and specific immunohistochemical marker for the Xp11 translocation RCC is nuclear labeling of TFE3 protein, which reflects over-expression of the resulting fusion proteins relative to native TFE3. The hallmark of Alveolar soft part sarcoma (ASPS) is a chromosomal rearrangement at 17q25 and Xp11.2 engendering an ASPSCR1-TFE3 fusion gene responsible for an aberrant transcription factor presumably enabling pathogenesis. The presence of this 'super-activated' transcription factor may induce the expression of numerous molecules contributing to ASPS diagnosis, progression, and metastasis. Uniprot ID: P19532 NCBI: NP_000526 GeneID: 7030 Host / Isotype: Rabbit / IgG Clone: MRQ-37 Immunogen: TFE3 peptide. Format: State: Liquid purified Ig fraction with 0.2% BSA and 15mM Sodium Azide. Applications: Immunohistochemistry on Formalin-Fixed and Paraffin-Embedded Sections: 1/25-1/100 Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Testis (germ cells) or Xp11 translocation renal cell carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Recognizes TFE3 Cellular Localization: Nuclear. Species Reactivity: Tested: Human. Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. RS/20150424 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 2 AM10141SU-N: Monoclonal Antibody to TFE3 - Supernatant Pictures: Formalin-Fixed, Paraffin-Embedded Human renal cell carcinoma stained with TFE3 antibody Cat.-No. AM10141SU-N using peroxidase conjugate and DAB chromogen. Note nuclear staining of tumor cells.