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Research Article ISSN 2336-9744 (online) | ISSN 2337-0173 (print) The journal is available on line at www.biotaxa.org/em https://zoobank.org/urn:lsid:zoobank.org:pub:D0D5B9BA-68A7-4047-B9A3-0B743BB0C288 New Phrurolithus species from China (Araneae, Phrurolithidae) LINA FU 1, HUIMING CHEN 2 & FENG ZHANG 1* 1The Key Laboratory of Invertebrate Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei 071002, P. R. China 2Institute of Biology, Guizhou Academy of Sciences, Guiyang 550009, China *Corresponding author. E-mail: [email protected] Received 19 June 2016 │ Accepted 24 July 2016 │ Published online 30 July 2016. Abstract Seven new spider species of the genus Phrurolithus C. L. Koch, 1839 are reported from China: P. celatus sp. nov. (♀♂), P. lasiolepis sp. nov. (♀♂), P. longus sp. nov. (♀♂), P. subannulus sp. nov. (♀♂), P. taoyuan sp. nov. (♀ ♂), P. validus sp. nov. (♀♂) and P. subnigerus sp. nov. (♀ ♂). Morphological illustrations, photos and descriptions of all new species are presented. Key words: spider, taxonomy, Phrurolithus, China. Introduction The sac spider family Phrurolithidae Banks 1892, elevated to family status by Ramírez (2014), currently consists of 207 species, 14 genera worldwide (World Spider Catalog 2016), and about 65 species in four genera have been found in China (Li & Wang 2015). They are hunting spiders living on the ground, and are usually found in leaf litter, woody debris or on the forest floor. The genus Phrurolithus was established by C. L. Koch (1839) based on two Palaearctic species, P. festivus (C.L. Koch, 1835) and P. minimus C.L. Koch, 1839. Phrurolithus, the most species-rich genus of Phrurolithidae (World Spider Catalog 2016), is recorded from Europe, Asia and North America (Holarctic). Among all 74 species, 21 species were found in China (Table 1). Currently, the polyphyly of Phrurolithus is delimited and the taxa may have multiple ancestral origins. For example, Wang et al. (2015) mentioned three groups within Phrurolithus: the Palaearctic group, the North American group, and the East and Southeast Asia group. The American Phrurolithus species, all possess a U-shaped RTA and a proximal femoral ventral apophysis (see Dondale & Redner 1982: fig. 296) and all may belong to the genus Scotinella (Wang et al., 2015). The genus is currently in need of world-wide revision. While examining the spider specimens collected from China, we found some Phrurolithus species new to science, and describe them here: P. celatus sp. nov., P. lasiolepis sp. nov., P. longus sp. nov., P. subannulus sp. nov., P. taoyuan sp. nov., P. validus sp. nov. and P. subnigerus sp. nov. Ecol. Mont., 7, 2016, 270-290 FU ET AL. Table 1. List of Phrurolithus species known from China. Number Species name Known Distribution Type Preservation sexes 1 P. annulus Zhou, Wang & ♂♀ Chongqing SWUC Zhang, 2013 2 P. anticus Wang et al., 2015 ♂♀ Guizhou SWUC 3 P. bifidus Yin et al., 2004 ♂♀ Yunnan HNU 4 P. cangshan Yang et al., 2010 ♂♀ Yunnan MHBU 5 P. claripes (Dönitz & Strand, ♂♀ Shanxi Japan 1906) 6 P. daoxianensis Yin et al., ♂ Hunan HNU 1997 number 98-NK-48 7 P. dianchiensis Yin et al., ♀ Hunan, Yunnan HNU 1997 number 98-NK-48 8 P. fanjingshan Wang et al., ♂♀ Guizhou SWUC 2015 9 P. festivus (C. L. Koch, 1835) ♂♀ Hebei, Henan, Germany 10 P. hamatus Wang, Zhang & ♂♀ Chongqingetc SWUC Zhang, 2012 11 P. nigerus Yin, 2012 ♀ Hunan HNU 12 P. palgongensis Seo, 1988 ♂♀ Liaoning Keimyung University 13 P. pennatus Yaginuma, 1967 ♂♀ Hunan, Zhejiang Otemon Gakuin 14 P. qiqiensis Yin et al., 2004 ♀ Yunnan HNUUniversity 15 P. shimenensis Yin et al., ♀ Hunan HNU 1997 number 98-NK-48 16 P. sinicus Zhu & Mei, 1982 ♂♀ Anhui, Shanxi, Bethune Medical 17 P. splendidus Song & Zheng, ♂♀ Zhejiangetc InstituteUniversity of Zoology 1992 18 P. wanshou Yin, 2012 ♂♀ Hunan HNU 19 P. zhejiangensis Song & Kim, ♀ Zhejiang In stitute of Zoology 1991 20 P. zhouyun Wang et al., 2015 ♂♀ Guizhou SWUC 21 P. zongxu Wang, Zhang & ♂♀ Chongqing SWUC Zhang, 2012 Material and Methods The terminology follows Hu & Zhang (2011) and Jäger & Wunderlich (2012). All measurements given in the text are in millimeters. Carapace length was measured from the anterior margin to the rear margin of the carapace medially. Eye sizes were measured as the maximum diameter in dorsal or frontal view. Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). Total length is the sum of the carapace and abdomen lengths, regardless of the petiolus. Epigynes were removed and cleared in a warm solution of 10% potassium hydroxide, transferred to alcohol and temporarily mounted for drawing. All specimens are preserved in 75% alcohol and were examined, drawn and measured under a Leica M205A stereomicroscope equipped with an Abbe drawing device. Photographs were taken using a Leica M205A stereomicroscope equipped with a DFC450 CCD. The specimens are deposited in the Museum of Hebei University, Baoding, China (MHBU). Ecol. Mont., 7, 2016, 270-290 271 NEW PHRUROLITHUS SPECIES FROM CHINA The following abbreviations are used: AER, anterior eye row; ALE, anterior lateral eyes; AME, anterior median eyes; BU, bursae; CD, copulatory duct; CO, copulatory openings; E, embolus; FA, femoral apophysis; FD, fertilization ducts; HNU, Hunan Normal University; MOA, median ocular area; MP, median plate; PER, posterior eye row; PLE, posterior lateral eyes; PME, posterior median eyes; PMS, posterior median spinnerets; PTA, prolateral tibial apophysis; RTA, retrolateral tibial apophysis; SD, sperm duct; SP, spermathecae; SWUC, Southwest University; TA, tegular apophysis. Taxonomy Phrurolithidae Banks, 1892 Phrurolithus C.L. Koch, 1839 Phrurolithus celatus sp. nov. Figs 1A–G, 2A–E Type material. Holotype ♂, China, Chongqing Municipality, Jiangjin District, Simian Mount Nature Reserve, Dawopu, Chaqi Hill (28°34.775′N, 106°21.116′E), 21 March 2012, Luyu WANG leg.; Paratypes: 1♀, same data as for holotype, 10 November 2011, Zongxu LI leg.; 1♀, Dawopu (28°34.302′N, 106°21.195′E), 20 March 2012, Luyu WANG leg.; 1♂, Dawopu, Chaqi Hill (28°34.775′N, 106°21.116′E), 6 March 2013, Xuankong JIANG leg.; 1♂, Chongqing Municipality, Nanchuan District, Jinshan Town, Lanbajing (29°00.535′N, 107°08.681′E), 10 March 2013, Xiangwei MENG leg. Etymology. The specific name is derived from the Latin word ‘celatus’, referring to the COs concealed posteriorly; adjective. Diagnosis. The female of the new species is similar to P. qiqiensis (Yin et al., 2004: 272, figs 8–10) in having two similar bursae, but can be distinguished from it by: 1) fertilization ducts curved (not curved in the latter); 2) spermathecae oval (not oval in the latter). The new species is similar to P. bifidus (Yin et al., 2004: 271, figs 1–7) in having a similar RTA, but can be distinguished from it by: 1) lower location of COs (high in the latter); 2) bursae larger (small in the latter); 3) spermathecae oval (not oval in the latter); 4) embolar base with a long, thin, membranous conductor (absent in the latter). Description. Male (Fig. 1A). Total length 2.39–2.56 (n=3). Holotype: body length 2.41; carapace 1.14 long, 0.96 wide; abdomen 1.17 long, 0.83 wide. Carapace yellowish, abruptly narrow forwards; fovea longitudinal, distinct. Eyes sizes and interdistances: AME 0.06, ALE 0.08, PME 0.05, PLE 0.06; AME– AME 0.02, AME–ALE 0.01, PME–PME 0.06, PME–PLE 0.06. MOA 0.16 long, front 0.13 wide, back 0.16 wide. Clypeus 0.09 high. Chelicerae have two strong anterior bristles, with three well-separated promarginal and five retromarginal closely related teeth. Labium and gnathocoxae wider than long, yellow. Legs light yellow. Measurements of legs: leg I 3.99 (1.08, 0.35, 1.12, 0.93, 0.51), II 3.46 (0.99, 0.32, 0.87, 0.76, 0.52), III 3.01 (0.82, 0.28, 0.61, 0.76, 0.54), IV 4.24 (1.22, 0.34, 0.92, 1.12, 0.64). Leg formula: 4123. Femora I–IV basally with one dorsal spine, femur I with three prolateral spines and femur II with one prolateral spine; tibia I with six pairs of ventral spines, tibia II with six proventral spines and five retroventral spines; metatarsus I with four proventral spines and three retroventral spines, metatarsus II with four proventral spines and three retroventral spines. Abdomen oval, grey, anterior half with a small dorsal scutum, posterior half light grey with several chevron-like stripes dorsally. Palp (Figs 1C–E, 2A–C). BTA, RTA long relatively with the same length. Embolus thin apically, originating prolaterally, with a long conductor near embolar base. Sperm duct U-shaped. TA small. Female (Fig. 1B). Total length 2.33–2.85 (n=4). One paratype: body 2.83 long; carapace 1.13 long, 0.97 wide; abdomen 1.63 long, 1.06 wide. Carapace light yellowish anteriorly; fovea longitudinal. Eyes sizes and interdistances: AME 0.06, ALE 0.08, PME 0.05, PLE 0.07; AME–AME 0.02, AME–ALE 0.01, PME– PME 0.08, PME–PLE 0.05. MOA 0.15 long, front 0.13 wide, back 0.16 wide. Clypeus 0.08 high. Leg measurements: I 3.69 (0.98, 0.31, 1.04, 0.91, 0.45); II 3.10 (0.84, 0.27, 0.82, 0.71, 0.46); III 2.87 (0.79, 0.25, 0.61, 0.69, 0.53); IV 4.24 (1.12, 0.38, 0.97, 1.13, 0.64). Leg formula: 4123. Femora I–IV with one dorsal spine. Femur I with three prolateral spines and femur II with two prolateral spines; tibia I with seven proventral spines or eight retroventral spines, tibia II with seven proventral spines and six retroventral spines; metatarsus I with four pairs of ventral spines, metatarsus II with four proventral spines and three retroventral spines.