DOCSLIB.ORG

Microfluidic Studies of Biological and Chemical Processes

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Microfluidic Studies of Biological and Chemical Processes Microfluidic Studies of Biological and Chemical Processes by Ethan Tumarkin A thesis submitted in conformity with the requirements for the degree of Doctor of Philosophy Department of Chemistry University of Toronto © Copyright by Ethan Tumarkin, 2012 Microfluidic Studies of Biological and Chemical Processes Ethan Tumarkin Doctor of Philosophy Department of Chemistry University of Toronto 2012 Abstract This thesis describes the development of microfluidic (MF) platforms for the study of biological and chemical processes. In particular the thesis is divided into two distinct parts: (i) development of a MF methodology to generate tunable cell-laden microenvironments for detailed studies of cell behavior, and (ii) the design and fabrication of MF reactors for studies of chemical reactions. First, this thesis presented the generation of biopolymer microenvironments for cell studies. In the first project we demonstrated a high-throughput MF system for generating cell-laden agarose microgels with a controllable ratio of two different types of cells. The MF co-encapsulation system was shown to be a robust method for identifying autocrine and/or paracrine dependence of specific cell subpopulations. In the second project we studied the effect of the mechanical properties on the behavior of acute myeloid leukemia (AML2) cancer cells. Cell-laden macroscopic agarose gels were prepared at varying agarose concentrations. A modest range of the elastic modulus of the agarose gels were achieved, ranging from 0.62 kPa to 20.21 kPa at room temperature. We observed a pronounced decrease in cell proliferation in stiffer gels when compared to the gels with lower elastic moduli. The second part of the thesis focuses on the development of MF platforms for studying chemical reactions. In the third project presented in this thesis, we exploited the temperature dependent solubility of CO2 in order to: (i) study the temperature mediated CO2 transfer between the gas and the various liquid phases on short time scales, and (ii) to generate bubbles with a dense layer of colloid particles (armoured bubbles). The fourth project involved the fabrication of a multi-modal MF device with integrated analytical probes. The MF device comprised a pH, temperature, and ATR-FTIR probes for in- situ analysis of chemical reactions in real-time. Furthermore, the MF reactor featured a temperature controlled feedback system capable of maintaining on-chip temperatures at flow rates up to 50 mL/hr. Key words: microfluidics, microenvironments, high-throughput, encapsulation, co-culture, biopolymers, elastic modulus, multi-modal, modular, integrated analytical probes, titrations III Imagination is more important than knowledge. Knowledge is limited. - Albert Einstein IV Acknowledgments First and foremost I want to give my outmost gratitude to my supervisor, mentor, and friend, Professor Eugenia Kumacheva. Without a doubt, her guidance, wisdom, enthusiasm, and constant support have shaped me into the person, teacher, and researcher that I am today. I am forever grateful for her insights about science and life. I am also extremely grateful to my committee members Professor Axel Guenther, Professor Gilbert Walker, and Professor Aaron Wheeler. In addition, I want to give my utmost appreciation to my collaborators Professor Axel Guenther, Professor Gilbert Walker, Professor Peter Zandstra, Professor Barbara Sherwood Lollar, Professor Mark Ungrin, Professor Zhihong Nie, and Professor Jesse Greener for their support and helpful discussions over the past 4 years. I want to thank all the persons I have had the pleasure of interacting with since I was a young undergraduate summer student during the summer of 2005. In no particular order, I want to say thank you to Dr. Hong Zhang, Dr. Mallika Das, Ilya Gourevich, Dr. Zhixiang Wei, Dr. Alla Petukhova, Dr. Chantal Paquet, Dr. Wei Li, Dr. Minseok Seo, Dr. Lindsey Fiddles, Andrew Paton, Dr. Ryan Simms, Michael Debono, Dr. Daniele Fava, Neta Raz, Dr. Raheem Peerani, Dan Voicu, Lsan Tzadu, Milad Abolhasani, Pasquale Benvenuto, Jörg Fochtmann, and Dr. Jemma Vickery. In the past 7 years I have had the pleasure of working with people which have become like family to me. Dr. Anna Lee, Yannick Bohren, Dr. Jai Il Park, Dr. Diego Velasco, Dr. Kun Liu, Dr. Slava Dubinsky, Miguel Neves, and Ivan Gorelikov have been like family watching over my shoulders and stepping in during difficult times. My life has been enriched because of them. Finally, I want to give thanks to my family. To my sister, I want to say thank you for always being supportive and helpful. Always know that I am very proud of you. To my father, I want to say thank you for your unparalleled belief in me, for your constant curiosity and willingness to listen to my research and my ideas, and for pushing me harder than anyone else. To my sister-in- law, thank you for always being there during good times and bad times; and of course for giving V me the most amazing niece in the world. To my brother, you are the glue that holds everything together. Many times I would have crumbled without your support. Brothers for life. To my mother, you are the guiding star I follow every day. Your light shines as bright today as it did when I was a child. It is with your love and wisdom that I am who I am today. To you I owe this manuscript. I promise you that I will always aspire for greatness, with my family and my life. I love you. VI Preface This thesis has been organized as a series of manuscripts (see the list below) which have been published in peer-reviewed scientific journals. As identified by primary authorship, all manuscripts were written by Ethan Tumarkin with critical comments and revision by Eugenia Kumacheva and corresponding collaborators. The contributions of other authors are provided in detail below. Chapter 1 Microfluidic Studies of Biological Phenomena The results in this chapter are mainly from manuscripts published in 1Chemical Society Reviews, 2009, 8, 2161, and 2Small, 2012, In Press Authors: 1Ethan Tumarkin, and Eugenia Kumacheva 2Diego Velasco, Ethan Tumarkin, and Eugenia Kumacheva Contributions: 1E. Tumarkin contributed to the article writing and figure design and preparation. 2E. Tumarkin contributed to the article writing and figure design and preparation. D. Velasco wrote the manuscript and helped with figure preparation. Chapter 3 High-Throughput Combinatorial Cell Co-Culture Using Microfluidics The results in this chapter are mainly from manuscripts published in Integrative Biology, 2011, 3, 653. Authors: Ethan Tumarkin, Lsan Tzadu, Elizabeth Csaszar, Minseok Seo, Hong Zhang, Anna Lee, Raheem Peerani, Kelly Purpura, Peter Zandstra, and Eugenia Kumacheva Contributions: E. Tumarkin contributed to the paper by designing and carrying out experiments, data analysis and interpretation, and article writing. L. Tzadu, M. Seo, and H. Zhang helped with microfluidic experiments. E. VII Csaszar, R. Peerani, and K. Purpura helped with cell culture, cell analysis, and interpretation. A Lee helped with the preparation of the manuscript. P. Zandstra provided guidance and suggestions on experimental design, and article writing. Chapter 6 Temperature-Controlled 'Breathing' of Carbon Dioxide Bubbles The results in this chapter are mainly from manuscripts published in 1Lab on a Chip, 2011, 11, 3545 and 2Chemical Communications, 2011, 47, 12712. Authors: 1Ethan Tumarkin,n, Zhihong Nie, Jai Il Park, Milad Abolhasani, Jesse Greener, Barbara Sherwood Lollar, Axel Guenther, and Eugenia Kumacheva 2Ethan Tumarkin, Zhihong Nie, Jai Il Park, and Eugenia Kumacheva Contributions: 1E. Tumarkin contributed to the paper by designing and carrying out experiments, data analysis and interpretation, and article writing. Z. Nie and J. Park help with experimental design and microfluidic experiments. M. Abolhasani assisted with simulation design and analysis. J. Greener performed ATR-FTIR experiments. B. Sherwood Lollar and A. Guenther provided guidance and suggestions on experimental design, data interpretation, and article writing. 2E. Tumarkin contributed to the paper by designing and carrying out experiments, data analysis and interpretation, and article writing. Z. Nie and J. Park help with experimental design and microfluidic experiments. Chapter 7 Development and Applications of Microfluidic Reactors with Multiple Reconfigurable Analytical Probes The results in this chapter are mainly from manuscripts published in 1Analyst, 2012, 137, 444 and 2Lab on a Chip, 2012, 12, 696. Authors: VIII 1Jesse Greener*, Ethan Tumarkin*, Michael Debono, Chi-Hang Kwan, Milad Abolhasani, Axel Guenther, and Eugenia Kumacheva, Co-First Author 2Jesse Greener, Ethan Tumarkin, Michael Debono, A. Dicks, and Eugenia Kumacheva Contributions: 1E. Tumarkin contributed to the paper by designing and carrying out experiments, data analysis and interpretation, and article writing. J. Greener contributed to the paper by designing and carrying out experiments, data analysis and interpretation, and article writing. M. Debono assisted with experiments. C.H. Kwan and M. Abolhasani assisted with simulation design and analysis. A. Guenther provided helpful guidance on experimental design and, as well as assisted with data interpretation and article writing. 2E. Tumarkin contributed to the paper by designing experiments, data analysis and interpretation, and supervision of M. Debono (undergraduate student). J. Greener contributed to the paper by designing experiments, data analysis and interpretation, and article writing. M. Debono performed
Load more

Recommended publications
  • Canadian Chemical News 33 Cscsection Bulletin Head SCC Materials Have Been Reported in the Globe of University Faculty Associations Award Worked with F
    CSCSection Bulletin head SCC the journal Nature involved tailored multi- valency in the design of a high avidity ligand that blocks the action of the Shiga like toxin responsible for the disease that result from pathogenic E. coli O157:H7. Bundle was the recipient of the Whistler Award in Carbohydrate Chemistry, awarded by the International Carbohy- drate Organization and the CSC’s R.U. Lemieux Award. Bundle currently serves on the editorial boards of Advances in Carbohydrate Chem- istry and Biochemistry, and Glycobiology Journal. He is a member of the advisory board of the Complex Carbohydrate Research Centre. He is the author of some and Ian Manners, FCIC. He moved on to has achieved broad international acclaim. 200+ scientific papers, book chapters and the University of British Columbia for Kumacheva’s reports on confinement- reviews, and patents. graduate studies in the lab of Stephen induced phase transitions in thin liquid Withers, FCIC. His PhD work, supported films was published in Science. Her work The Boehringer Ingelheim by NSERC and Killam Fellowships, focused on the new mechanism of lubrication by Award for Organic or on the mechanisms and engineering of a polymer brushes was published in Nature- class of enzymes called glycosidases. Kumacheva’s studies of supramolecular Bioorganic Chemistry / After obtaining his PhD in 2001, he con- assemblyof rigid-rod polymers shed light Le Prix de chimie organique ducted postdoctoral research in X-ray on the mechanisms of fibrogenesis of ou bio-organique Boehringer crystallography with Gideon Davies ofYork proteins. Her studies of forces acting Ingelheim Structural Biology Laboratory, U.K.
    [Show full text]
  • Smutty Alchemy
    University of Calgary PRISM: University of Calgary's Digital Repository Graduate Studies The Vault: Electronic Theses and Dissertations 2021-01-18 Smutty Alchemy Smith, Mallory E. Land Smith, M. E. L. (2021). Smutty Alchemy (Unpublished doctoral thesis). University of Calgary, Calgary, AB. http://hdl.handle.net/1880/113019 doctoral thesis University of Calgary graduate students retain copyright ownership and moral rights for their thesis. You may use this material in any way that is permitted by the Copyright Act or through licensing that has been assigned to the document. For uses that are not allowable under copyright legislation or licensing, you are required to seek permission. Downloaded from PRISM: https://prism.ucalgary.ca UNIVERSITY OF CALGARY Smutty Alchemy by Mallory E. Land Smith A THESIS SUBMITTED TO THE FACULTY OF GRADUATE STUDIES IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY GRADUATE PROGRAM IN ENGLISH CALGARY, ALBERTA JANUARY, 2021 © Mallory E. Land Smith 2021 MELS ii Abstract Sina Queyras, in the essay “Lyric Conceptualism: A Manifesto in Progress,” describes the Lyric Conceptualist as a poet capable of recognizing the effects of disparate movements and employing a variety of lyric, conceptual, and language poetry techniques to continue to innovate in poetry without dismissing the work of other schools of poetic thought. Queyras sees the lyric conceptualist as an artistic curator who collects, modifies, selects, synthesizes, and adapts, to create verse that is both conceptual and accessible, using relevant materials and techniques from the past and present. This dissertation responds to Queyras’s idea with a collection of original poems in the lyric conceptualist mode, supported by a critical exegesis of that work.
    [Show full text]
  • (Bio) Particle Analysis Using Droplet Microfluidic Platform
    Strategic design of flow structures for single (bio) particle analysis using droplet microfluidic platform by Thu Hoang Anh Nguyen A thesis presented to the University of Waterloo in fulfillment of the thesis requirement for the degree of Doctor of Philosophy in Mechanical and Mechatronics Engineering (Nanotechnology) Waterloo, Ontario, Canada, 2019 © Thu Hoang Anh Nguyen 2019 Examining Committee Membership The following served on the Examining Committee for this thesis. The decision of the Examining Committee is by majority vote. External Examiner: Associate Professor Carlos Escobedo Department of Chemical Engineering Faculty of Engineering and Applied Science Queen’s University, Ontario, Canada Supervisor: Professor Carolyn L. Ren Department of Mechanical and Mechatronics Engineering Faculty of Engineering University of Waterloo, Ontario, Canada Internal Member: Professor David Johnson Department of Mechanical and Mechatronics Engineering Faculty of Engineering University of Waterloo, Ontario, Canada Internal/External Member: Professor Shirley Tang Department of Chemistry Faculty of Science University of Waterloo, Ontario, Canada Internal/External Member: Associate Professor Shawn Wettig School of Pharmacy University of Waterloo, Ontario, Canada ii AUTHOR'S DECLARATION I hereby declare that I am the sole author of this thesis. This is a true copy of the thesis, including any required final revisions, as accepted by my examiners. I understand that my thesis may be made electronically available to the public. iii Abstract Over the past two decades, a growing body of literature has recognized the importance of droplet-based microfluidics. This advanced technology shows promise in enabling large scale interdisciplinary studies that require high throughput and accurate manipulation of reagents. Each droplet acts as a micro-sized reactor where complex reactions can be carried out on the micro-scale by splitting, mixing, sorting and merging droplets.
    [Show full text]
  • CV__Wei Li-Che-TTU-July 2019
    Wei Li Assistant Professor Phone: 806-834-2209 Department of Chemical Engineering Fax: 806-742-3552 Texas Tech University E-mail: [email protected] Lubbock, TX 79409 Website: http://www.depts.ttu.edu/che/groups/ligroup/index.htm Education • Doctor of Philosophy, Department of Chemistry, University of Toronto, Canada. Sept.2005– June 2010 Advisor: Prof. Eugenia Kumacheva • Master of Applied Science, Department of Chemical Engineering, University of Toronto, Canada. Sept. 2003- June 2005 Advisor: Prof. Yu-Ling Cheng • Master of Science, Department of Chemistry, Wuhan University, China, Sept.1999-June 2002 Advisor: Prof. Renxi Zhuo • Bachelor of Science, Department of Chemistry, Wuhan University, China, Sept 1995- June1999 Appointments Jan. 2014-Present Assistant Professor Institute: Department of Chemical Engineering Texas Tech University, Lubbock, TX, USA Research Areas: Responsive LbL nanofilms, Cell capture and release, Polyelectrolyte hydrogels, Exosomes, Cell microenvironments, Biomedical microdevices Nov. 2010- Oct. 2013 NSERC Postdoctoral Research Fellow Institute: Department of Chemical Engineering, MIT, Cambridge, MA, USA Research Areas: LbL nanofilms, microfluidic devices for capture and release of cancer cells, 3D cell microenvironments, Advisor: Prof. Paula T. Hammond 1 Honors and Awards • WCOE Whitacre Research Award (2017) • NSERC Postdoctoral Fellowship (2010) • Chinese Government Award for Outstanding Students Abroad (2009) • Ontario Graduate Scholarships in Science and Technology (2008) • Edwin Walter Warren Graduate Student Awards (2007, 2008) • Xerox Research Centre of Canada Graduate Award (2007) • Ontario Centers of Excellence Professional Outreach Award (2007) • Graduate Travel Award, University of Toronto (2009) • Open Fellowship, University of Toronto (2003-2007) • Outstanding Graduate Student, Wuhan University, (2000-2002) Sponsored Projects External • Utilizing glycoside hydrolases to degrade biofilms in wounds.
    [Show full text]
  • BIOLOGY 639 SCIENCE ONLINE the Unexpected Brains Behind Blood Vessel Growth 641 THIS WEEK in SCIENCE 668 U.K
    4 February 2005 Vol. 307 No. 5710 Pages 629–796 $10 07%.'+%#%+& 2416'+0(70%6+10 37#06+6#6+8' 51(69#4' #/2.+(+%#6+10 %'..$+1.1); %.10+0) /+%41#44#;5 #0#.;5+5 #0#.;5+5 2%4 51.76+105 Finish first with a superior species. 50% faster real-time results with FullVelocity™ QPCR Kits! Our FullVelocity™ master mixes use a novel enzyme species to deliver Superior Performance vs. Taq -Based Reagents FullVelocity™ Taq -Based real-time results faster than conventional reagents. With a simple change Reagent Kits Reagent Kits Enzyme species High-speed Thermus to the thermal profile on your existing real-time PCR system, the archaeal Fast time to results FullVelocity technology provides you high-speed amplification without Enzyme thermostability dUTP incorporation requiring any special equipment or re-optimization. SYBR® Green tolerance Price per reaction $$$ • Fast, economical • Efficient, specific and • Probe and SYBR® results sensitive Green chemistries Need More Information? Give Us A Call: Ask Us About These Great Products: Stratagene USA and Canada Stratagene Europe FullVelocity™ QPCR Master Mix* 600561 Order: (800) 424-5444 x3 Order: 00800-7000-7000 FullVelocity™ QRT-PCR Master Mix* 600562 Technical Services: (800) 894-1304 Technical Services: 00800-7400-7400 FullVelocity™ SYBR® Green QPCR Master Mix 600581 FullVelocity™ SYBR® Green QRT-PCR Master Mix 600582 Stratagene Japan K.K. *U.S. Patent Nos. 6,528,254, 6,548,250, and patents pending. Order: 03-5159-2060 Purchase of these products is accompanied by a license to use them in the Polymerase Chain Reaction (PCR) Technical Services: 03-5159-2070 process in conjunction with a thermal cycler whose use in the automated performance of the PCR process is YYYUVTCVCIGPGEQO covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler.
    [Show full text]
  • Thesis Title Goes Here
    FACTORS AFFECTING THE GROWTH AND FRAGMENTATION OF POLYFERROCENYLSILANE DIBLOCK COPOLYMER MICELLES By Jieshu Qian A thesis submitted in conformity with the requirements for the degree of Doctor of Philosophy Graduate Department of Chemistry University of Toronto © Copyright by Jieshu Qian, 2013 FACTORS AFFECTING THE GROWTH AND FRAGMENTATION OF POLYFERROCENYLSILANE DIBLOCK COPOLYMER MICELLES Jieshu Qian Doctor of Philosophy Graduate Department of Chemistry University of Toronto 2013 Abstract Polyferrocenylsilane (PFS) diblock copolymers self-assemble in selective solvents to form one-dimensional micelles for a broad range of polymer compositions and experimental conditions, driven by the crystallization of the PFS block that forms the micelle core. The most striking feature of these micelles is that they remain active for further growth. They can be extended in length when additional polymer, dissolved in a good solvent, is added to a solution of the pre-existing micelles. This thesis describes several studies investigating the factors that affect the growth and fragmentation of PFS diblock copolymer micelles in solution, with a particular emphasis on polyisoprene-PFS (PI-PFS) diblock copolymers. The goal of my research was trying to provide deeper understanding of this crystallization-driven self-assembly (CDSA) process. In an attempt to understand the growth kinetics of the PI-PFS cylindrical micelles, I added tiny amount of short micelle seeds into supersaturated solution of the same polymer, and followed the micelle growth by light scattering. The data analysis showed that the increase of micelle length could be described by an expression with two exponential decay terms. In another ii attempt to examine the factors that may affect the growth behavior of the PI-PFS micelles, I found that PI-PFS long micelles underwent fragmentation when they were subjected to external stimuli, e.g.
    [Show full text]
  • 2007 2007 Ontario Chapter Officers in THIS ISSUE
    135 Shuh Avenue Volume XXVI Kitchener, ON, N2A 1H4 January 2007 2007 Ontario Chapter Officers IN THIS ISSUE... Chair Monica Melao • Charles Tennant & Company CHAPTER MEETING…...….……………….…....... page 1,2,3 34 Clayson Rd. • A FAREWELL MESSAGE FROM THE CHAIR……….. page 4 Weston, ON M9M 2G8 Ph: (416) 741-9264 • JOB SEARCH………………………………………….. page 5 Fax: (416) 741-6642 • TRIVIA……………………………………….………. .. page 6 [email protected] • CASINO & SILENT AUCTION NIGHT……………… page 9 Chair-Elect • WANT TO WIN SOME MAPLE LEAF TICKETS?... .. page 10 Gagan Jain Ashland Canada Corp. • HOLIDAY DINNER DANCE……………………….. .. page 12 2463 Royal Windsor Drive • UPCOMING EVENTS………………………………… page 17 Mississauga, Ontario L5J 1K9 Ph: 877-404-4437 Fax: 905-823-0023 [email protected] PRE-REGISTRATION IS REQUIRED FOR FEBRUARY MEETING Secretary If you prefer you can reserve a spot by email or fax. Send us a Andrea Hopp note that you will be there. We will confirm receipt of your reser- Croda Canada Ltd 221A Racco Parkway vation. You can then either bring the payment with you or put it in Vaughan, ON L4J 8X9 the mail. Ph: 905-886-1383 Fax: 905-886-4753 Please make your reservation prior to February 5th , 2007. [email protected] Treasurer Please note: Gary Baker Should you register and not attend you will be invoiced the meeting fee. GE&B Marketing Students must have their payment in by Monday prior to the meeting. 135 Shuh Avenue To make your reservation today contact Gary at: [email protected] Kitchener, ON N2A 1H4 Ph: (519) 896-1168 or by fax at (519) 896-7350 (If registering by mail please use the form on the Fax: (519) 896-7350 back page) [email protected] Directors Area II Jim Vlasic Ph: 952-707-6904 Fax: 952-808-0213 Continuous Microfluidic Reactors [email protected] for Chris Heisig Polymer Particles Ph: (314) 290-4747 Eugenia Kumacheva Fax: (314) 290-4605 [email protected] Department of Chemistry, University of Toronto Past Chair Dorothy Maraprossians Linker-based lecithin microemulsions Amisol, A Division of Multichem Edgar J.
    [Show full text]
  • TECHNICAL PROGRAM 90Th ACS Colloid and Surface Science Symposium June 5-8, 2016
    Welcome message from 90th CSSS Co-Chairs David Weitz Joyce Wong Ramanathan Nagarajan Harvard University Boston University Natick Soldier RDEC Welcome to Harvard University and to the 90th Colloid and Surface Science Symposium. Harvard is the oldest institution of higher education in the United States, established in 1636. Harvard has been an active international center for work in various aspects of colloid and surface science, both traditional and modern, and is pleased to host the 90th ACS Colloid and Surface Science Symposium. The Harvard CSSS will highlight advances in colloid and surface science and its intersections with diverse scientific and technological domains, through nearly 600 technical presentations, organized into 13 topical symposia, a general papers symposium and a poster session. Plenary lectures will be presented by Prof. Françoise Brochard-Wyart from Institut Curie, Paris, France and Prof. Eugenia Kumacheva from the University of Toronto, Canada. There will also be plenary award lectures by Prof. Matthew Helgeson, UC Santa Barbara, the recipient of the 2016 Unilever Award and Prof. Michelle Personick, Wesleyan University, the recipient of the 2016 Victor K. LaMer Award As has been an important part of this summer symposium over the years, there will be an instrument exhibition highlighting advances in instrumental techniques in colloid and surface science. Recognition of student researchers is another highlight of this meeting. The 90th CSSS will feature awards for best graduate student oral presentations, sponsored by the ACS journal Langmuir. These awards aim to recognize excellence in research as manifested in oral presentations of pre-selected graduate student presenters in the “Langmuir Graduate Student Awards Session”.
    [Show full text]
  • CV-Jan 2017-Wei Li on Webpage
    Wei Li Current Position (Jan. 2014-present) Assistant Professor Department of Chemical Engineering Texas Tech University, PO Box 43121, Lubbock, TX, 79409-3121 Phone: 806-834-2209 Web page: http://www.depts.ttu.edu/che/groups/ligroup/index.htm Email: [email protected] EDUCATION NSERC Postdoctoral Research Fellow 2010- 2013 Department of Chemical Engineering, MIT Advisor: Prof. Paula T. Hammond Doctor of Philosophy 2005–2010 Department of Chemistry, University of Toronto, Canada Advisor: Prof. Eugenia Kumacheva Master of Applied Science 2003-2005 Department of Chemical Engineering, University of Toronto, Canada Advisor: Prof. Yu-Ling Cheng Master of Science 1999-2002 Department of Chemistry, Polymer Chemistry & Physics, Wuhan University, China Advisor: Prof. Ren-Xi Zhuo Bachelor of Science 1995-1999 Department of Chemistry, Wuhan University, China, 1999 AWARDS • NSERC Postdoctoral Fellowship (2010) • Chinese Government Award for Outstanding Students Abroad (2009) • Ontario Graduate Scholarships in Science and Technology (2008) • Edwin Walter Warren Graduate Student Awards (2007, 2008) • Xerox Research Centre of Canada Graduate Award (2007) • Ontario Centers of Excellence Professional Outreach Award (2007) • Graduate Travel Award, University of Toronto (2009) • Open Fellowship, University of Toronto (2003-2007) • Outstanding Graduate Student, Wuhan University, (2000-2002) 1 PUBLICATIONS Researcher ID: P-3546-2016, h-index 15 with citation of 1780 by March 2017 [Google Scholar link: https://scholar.google.com/citations?user=E3XTpssAAAAJ&hl=en] * corresponding author, ‡ undergraduate student. Publications in Refereed Journals 26. Z. Dong, C. C. Ahrens, D. Yu, Z. Ding, H.T. Lim, W. Li.* Cell isolation and recovery in resource-limited settings using hollow glass microspheres coated with nanolayered films.
    [Show full text]
  • Investigation of Nano-Scale, Self-Assembled, Polymeric Systems by Atomic Force Microscopy
    Investigation of Nano-Scale, Self-Assembled, Polymeric Systems by Atomic Force Microscopy by James K. Li A thesis submitted in conformity with the requirements for the degree of Doctor of Philosophy Department of Chemistry University of Toronto © Copyright by James K. Li 2010 ii Atomic Force Microscopy of Nano-Scale, Self-Assembled, Polymeric Systems James K. Li Doctor of Philosophy Department of Chemistry University of Toronto 2010 Abstract The atomic force microscope (AFM) was used to study a series of self-assembled systems: alkanethiol self-assembled monolayer (SAM), diblock copolymer thin film, solid supported lipid bilayer membrane, and microgel with double interpenetrating polymer network. In the first system, packing and restructuring of self-assembled monolayers as exhibited by several alkanethiol systems (1-hexanethiol, 1-decanethiol, 11-ferrocenyl-1-undecanethiol) is demonstrated using conducting probe AFM (CP-AFM). Pressure is induced by an AFM tip, and simultaneously, electrical behavior is measured via detection of tunneling currents between metallic tip and substrate. The behavior is fit using a mechanical model that attempts to predict the observed junction resistance as a function of applied force with consideration for mechanical restructuring of the monolayer at higher loads. CP-AFM is also used to study self-assembled thin film of the diblock copolymer polystyrene- block-polyferrocenylsilane (PS-b-PFS) on gold substrate. Simultaneous height and electrical iii current imaging verify the phase separation of the two blocks of the polymer and additionally, distinguish each block due to differential conductivity. The phase separation of multi-component phospholipid bilayers (phosphatidylcholine/ sphingomyelin/ cholesterol) on supporting substrate into liquid-ordered and liquid-disordered phases is demonstrated using both topographical imaging, and the use of force map analysis through tip indentation and rupture measurements.
    [Show full text]
  • Trustees' Report and Financial Statements
    Trustees’ report and financial statements For the year ended 31 March 2017 2 TRUSTEES’ REPORT AND FINANCIAL STATEMENTS Trustees Executive Director The Trustees of the Society are the members of its Council, Dr Julie Maxton who are elected by and from the Fellowship. Council is chaired by the President of the Society. During 2016/17, Key Management Personnel the members of Council were as follows: Jennifer Cormack, Director of Development Dr Claire Craig, Director of Science Policy President Mary Daly, Chief Financial Officer Sir Venki Ramakrishnan Bill Hartnett, Director of Communications Dr Paul McDonald, Director of Grants Programmes Treasurer Lesley Miles, Chief Strategy Officer Professor Anthony Cheetham Dr Stuart Taylor, Director of Publishing Dr David Walker, Executive Assistant to the Executive Director Physical Secretary and Governance Officer Professor Alexander Halliday Rapela Zaman, Director of International Affairs Foreign Secretary Professor Richard Catlow** Statutory Auditor Sir Martyn Poliakoff* BDO LLP 2 City Place Biological Secretary Beehive Ring Road Sir John Skehel Gatwick West Sussex Members of Council RH6 0PA Professor Gillian Bates** Professor Jean Beggs** Bankers Professor Andrea Brand* The Royal Bank of Scotland Sir Keith Burnett 1 Princes Street Professor Eleanor Campbell** London Professor Michael Cates* EC2R 8BP Professor George Efstathiou Professor Brian Foster Investment Managers Professor Russell Foster** Rathbone Brothers PLC Professor Uta Frith 1 Curzon Street Professor Joanna Haigh London Dame Wendy Hall*
    [Show full text]
  • Acknowledgment of Reviewers, 2014
    Acknowledgment of Reviewers, 2014 The PNAS editors would like to thank all the individuals who dedicated their considerable time and expertise to the journal by serving as reviewers in 2014. Their generous contribution is deeply appreciated. A Joseph Adams Seungkirl Ahn Hashim Al-Hashimi Luis Amaral Kjersti Aagard-Tillery Michael Adams Tero Ahola Javey Ali Rommie Amaro Duur Aanen Paul Adams Cyrus Aidun Antonios Aliprantis Bruno Amati Jorge Abad Ralf Adams Iannis Aifantis Kari Alitalo Jayakrishna Ambati Alejandro Aballay Lee Adamson Kazuyuki Aihara Rob Alkemade Richard Ambinder Adam Abate John Adelman Judd Aiken Michael Alkire Stanley Ambrose Alireza Abbaspourrad Karen Adelman Elizabeth Ainsworth Robin Allaby Indu Ambudkar Jonathan Abbatt Zach Adelman William Aird Milan P. Allan Chris Amemiya Patrick Abbot Pia Ädelroth Edoardo Airoldi Marc Allard Jan Amend Albert Abbott Sarah Ades Joanna Aizenberg Hunt Allcott Amal Amer Allison Abbott Ilensami Adesida Michael Aizenberg Martin Allday Stefan Ameres Derek Abbott Becky Adkins Myles Akabas Andrew Allen Sebastian Amigorena Larry Abbott Elizabeth Adkins-Regan Ilke Akartuna Charles Allen Mohammed Amin Nicholas Abbott Andy Adler Erol Akcay Dale Allen Ido Amit Paul Abbyad Frederick Adler Mark Akeson David Allen Gabriel Amitai Omar Abdel-Wahab Gregory Adler Christopher Akey Eric Allen Sygal Amitay Yalchin Abdullaev Lynn Adler Ethan Akin Irving Allen Markus Ammann Ikuro Abe Roee Admon Shizuo Akira James Allen David Amodio Jun-ichi Abe Ralph Adolphs Gustav Akk John Allen Angelika Amon Koji Abe Jose Adrio Mikael Akke Karen Allen Christopher Amos Goncalo Abecasis Radoslav Adzic Serap Aksoy Melinda Allen Linda Amos Stephen Abedon Markus Aebi Anastasia Aksyuk Nicola Allen Derk Amsen Markus Abel Toni Aebischer Klaus Aktories Paul Allen Ronald Amundson Moshe Abeles G.
    [Show full text]