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Environmental Method Development: Key Challenges

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Environmental Method Development: Key Challenges Volume 37 Number 7 July 2019 www.chromatographyonline.com A Revolutionary Technology for Macromolecular Characterization A New Paradigm in Light Scattering Technology • Direct Molecular Weight by Low Angle • HPLC/UHPLC Compatibility • Rg Below 10 nm for the First Time • Powerful and Intuitive Software LenS3 in UHPLC Mode: Herceptin® Biosimilar (150 KDa) TSKgel UP-SW3000, 2 μm, 4.6 mm ID x 30 cm 1800 10,000,000 Concentration = 2.75 mg/mL Concentration = 0.0021-0.0105 mg/mL 5,000,000 6 1600 30 Injection volume = 5 μL Injection volume = 1-2 μL 1400 Monomer 5 25 1200 1,000,000 4 RALS (mV) 20 Log(MW) 1000 500,000 Monomer Fragment 800 3 UV (mV) 15 10 ng RALS (mV) 600 100,000 2 10 4 ng 400 50,000 Fragment 1 5 Dimer 200 Aggregates 2 ng 10,000 0 4.75 5 5.25 5.5 5.75 6 6.25 6.5 6.75 7 7.25 7.5 7.75 8 8.25 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 9.5 10 Retention time (minutes) Retention time (minutes) Temperature- ENVIRONMENTAL How Buffer ProgrammedmAb GC: monomer, fragment, oligomers and aggregate detectionPreparation with sensitivity down to only 2 ng! Why Are Those METHOD Affects LC Peaks All So DEVELOPMENT: Retention Sharp? Repeatability www.tosohbioscience.comKEY CHALLENGES magentablackcyanyellow ES100702_LCGC0719_CVTP1_FP.pgs 07.03.2019 17:34 UBM See Your Molecule in a Whole New Light A Winning Combination for Your Polymer Analysis Delivering greater stability and reproducibility than ever before. Supported by team of GPC experts. Driven by SECview Software TM Actualization, not Extrapolation of Your Data For more information visit tosohbioscience.com Tosoh Bioscience, EcoSEC Elite, and TSKgel are registered trademarks of Tosoh Corporation. LenS and SECview are trademarks of Tosoh Bioscience LLC. Herceptin is a registered trademark of Genentech, Inc. www.tosohbioscience.com magentablackcyanyellow ES100704_LCGC0719_CVTP2_FP.pgs 07.03.2019 17:34 UBM Volume 37 Number 7 July 2019 www.chromatographyonline.com Temperature- ENVIRONMENTAL How Buffer Programmed GC: Preparation Why Are Those METHOD Affects LC Peaks All So DEVELOPMENT: Retention Sharp? KEY CHALLENGES Repeatability Supelco® titration products. Exquisite accuracy. Every time. Maintaining your high standards requires analytical products you can rely on, along with the right documentation and expertise. That's why our titration reagents, solvents, reference materials and indicators are exquisitely crafted to ensure you reliably achieve your analytical goals and comply with guidelines and regulations. So for fl awless performance, time after time, choose Supelco® titration products. For more information, please visit: SigmaAldrich.com/SuccessReplicated MilliporeSigma, the vibrant M and Supelco are trademarks of Merck KGaA, Darmstadt, Germany or its affi liates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources. © 2019 Merck KGaA, Darmstadt, Germany and/or its affi liates. All Rights Reserved. The Life Science Business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada. The power of polymeric columns Polymer HPLC columns have a lot of benefits. They don’t require any functionalization for reversed-phase separations, and rigid polymeric supports intrinsically resist chemical and pH degradation, a fundamental problem with silica columns. Plus, polymer’s inertness to most chemical environments makes it a robust and economical solution. Hamilton offers a line of pH stable polymer HPLC columns for reversed phase, anion exchange, cation exchange, and ion exclusion separations perfect for pharmaceuticals, small molecules, proteins, peptides, DNA, organic, and inorganic ions, and more. Advantages of polymer over silica pH range of 1 – 13 Widest chemical compatibility Temperatures higher than 60 °C Maximum sample recovery Longest average life span To learn more about how polymer columns can perform for you, visit www.hamiltoncompany.com/polymers-101 or call toll free 1-888-525-2123 © 2018 Hamilton Company. All rights reserved. Images Copyright Rangizzz and Carolina K. Smith, M.D., 2018 Used under license from Shutterstock.com 428 LCGC NORTH AMERICA VOLUME 37 NUMBER 7 JULY 2019 WWW.CHROMATOGRAPHYONLINE.COM MANUSCRIPTS: For manuscript preparation guidelines, see chromatographyon- 485F US Highway One South, Suite 210 line.com/lcgc-author-guidelines, or call The Editor, (732) 596-0276. 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Decade Elite Electrochemical detector for any (U)HPLC system with the software of your choice www.AntecScientific.com 430 LCGC NORTH AMERICA VOLUME 37 NUMBER 7 JULY 2019 WWW.CHROMATOGRAPHYONLINE.COM CONTENTS COLUMNS 436 COLUMN WATCH Hybrid Particle Columns: The First Twenty Years Thomas H. Walter We examine the evolution of hybrid particle columns, highlighting examples of separations enabled by this technology.
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