Risk of Human Pathogen Internalization in Leafy Vegetables During Lab-Scale Hydroponic Cultivation
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Human Illness Caused by E. Coli O157:H7 from Food and Non-Food Sources
FRI BRIEFINGS Human Illness Caused by E. coli O157:H7 from Food and Non-food Sources M. Ellin Doyle1*, John Archer2, Charles W. Kaspar1, and Ronald Weiss1 1Food Research Institute, University of Wisconsin–Madison, Madison, WI 53706 2Wisconsin Division of Public Health, Bureau of Communicable Diseases and Preparedness, Communicable Disease Epidemiology Section, Madison, WI 53702 Contents Introduction ...................................................................................................................................2 Epidemiology of E. coli O157:H7..................................................................................................2 Outbreak Data ........................................................................................................................2 Reservoirs of E. coli O157:H7 ..............................................................................................3 Cattle—the primary reservoir ........................................................................................3 Other ruminants .............................................................................................................4 Other animals .................................................................................................................4 Transport Hosts......................................................................................................................4 Routes of Human Infection ....................................................................................................5 -
How Do Pathogenic Microorganisms Develop Cross-Kingdom Host Jumps? Peter Van Baarlen1, Alex Van Belkum2, Richard C
Molecular mechanisms of pathogenicity: how do pathogenic microorganisms develop cross-kingdom host jumps? Peter van Baarlen1, Alex van Belkum2, Richard C. Summerbell3, Pedro W. Crous3 & Bart P.H.J. Thomma1 1Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands; 2Department of Medical Microbiology and Infectious Diseases, Erasmus MC, University Medical Centre Rotterdam, Rotterdam, The Netherlands; and 3CBS Fungal Biodiversity Centre, Utrecht, The Netherlands Correspondence: Bart P.H.J. Thomma, Abstract Downloaded from https://academic.oup.com/femsre/article/31/3/239/2367343 by guest on 27 September 2021 Laboratory of Phytopathology, Wageningen University, Binnenhaven 5, 6709 PD It is common knowledge that pathogenic viruses can change hosts, with avian Wageningen, The Netherlands. Tel.: 10031 influenza, the HIV, and the causal agent of variant Creutzfeldt–Jacob encephalitis 317 484536; fax: 10031 317 483412; as well-known examples. Less well known, however, is that host jumps also occur e-mail: [email protected] with more complex pathogenic microorganisms such as bacteria and fungi. In extreme cases, these host jumps even cross kingdom of life barriers. A number of Received 3 July 2006; revised 22 December requirements need to be met to enable a microorganism to cross such kingdom 2006; accepted 23 December 2006. barriers. Potential cross-kingdom pathogenic microorganisms must be able to First published online 26 February 2007. come into close and frequent contact with potential hosts, and must be able to overcome or evade host defences. Reproduction on, in, or near the new host will DOI:10.1111/j.1574-6976.2007.00065.x ensure the transmission or release of successful genotypes. -
What Better Plants Are Grown on Grown Are Plants Better What
GroWSToNe what better plants are grown on 100% Recycled. 100% American Made. www.growstone.com 100% Recycled. 100% American Made. Growstone Hydroponic Growth Mediums and Super Soil Aerators are a horticultural, scientific and environmental win-win-win. Growstones are an effective alternative to perlite and Hydroton®. They guarantee a considerably higher aeration and faster drainage than perlite, while simultaneously holding more water than Hydroton. Growstones provide: Other Growstones characteristics are: 3 times more water than Hydroton® High porosity and aeration Over 70% more aeration than perlite Proven yields ‘as-good-as’ the industry standards 4 times more aeration than coco coir Can satisfy a wide range of plant requirements for different growing systems and climates Made from 100% recycled glass The perfect balance of air and water Not strip-mined like Hydroton® and perlite Growstones highly porous media associate a good water holding Inert capacity to high air-filled porosity. At field capacity, Growstones pH neutral after preparation hold 30% water within its pores and 50% air by volume within its 100% non-toxic and chemical free inner and inter aggregate spaces. This water to air ratio maximizes Lightweight, dust-free water availability and aeration to your plant roots. It also Impossible to over water facilitates drainage making it impossible to over irrigate. Does not float to top when irrigated Non-degradable Non-compacting Average Water Holding Capacity Reusable Air Filled Porosity of Substrates After Drainage Made in the USA “What growers like about “Our Rocks Growstones are they hold a lot of oxygen and moisture. Don’t Roll” And the fact they are recycled —Andrew makes them even better.” Founder & Inventor of Growstone Santa Fe, New Mexico —Dylan | Deep Roots Hydroponics Garden Supply | Sebastopol, California Propagation How to Use Growstones Place your seeds in any propagation media. -
Differences in Innate Immune Response Between Man and Mouse
Institute of Medical Physics and Biophysics Medical Department, Leipzig University Author Manuscript © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ Published in final edited form as: Critical Reviews™ in Immunology, 2014; 34:433-54. Available at: http://dx.doi.org/ 10.1615/CritRevImmunol.2014011600. DIFFERENCES IN INNATE IMMUNE RESPONSE BETWEEN MAN AND MOUSE Josefin Zschaler 1,2,*, Denise Schlorke 1,2,* & Juergen Arnhold 1,2 1 Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Leipzig, Germany 2 Translational Centre for Regenerative Medicine (TRM), Leipzig University, Leipzig, Germany * Both authors contributed equally to this work. Abstract Mouse strains are frequently used to model human disease states, to test the efficiency of drugs and therapeutic principles. However, the direct translation of murine experimental data to human pathological events often fails due to sufficient differences in the organization of the immune system of both species. Here we give a short overview of the principle differences between mice and humans in defense strategies against pathogens and mechanisms involved in response to pathogenic microorganisms and other activators of the immune system. While in human blood mechanisms of immune resistance are highly prevailed, tolerance mechanisms dominate for the defense against pathogenic microorganisms in mouse blood. Further on, species-related differences of immune cells mainly involved in innate immune response as well as differences to maintain oxidative homeostasis are also considered. A number of disease scenarios in mice are critically reflected for their suitability to serve as a model for human pathologies. -
The Public Health Impact of Prion Diseases1
10 Feb 2005 13:10 AR AR238-PU26-08.tex AR238-PU26-08.sgm LaTeX2e(2002/01/18) P1: IBD 10.1146/annurev.publhealth.26.021304.144536 Annu. Rev. Public Health 2005. 26:191–212 doi: 10.1146/annurev.publhealth.26.021304.144536 Copyright ©c 2005 by Annual Reviews. All rights reserved First published online as a Review in Advance on December 8, 2004 THE PUBLIC HEALTH IMPACT OF PRION DISEASES1 Ermias D. Belay and Lawrence B. Schonberger Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333; email: [email protected] KeyWords transmissible spongiform encephalopathy, Creutzfeldt-Jakob disease, variant Creutzfeldt-Jakob disease, bovine spongiform encephalopathy, chronic wasting disease ■ Abstract Several prion disease–related human health risks from an exogenous source can be identified in the United States, including the iatrogenic transmission of Creutzfeldt-Jakob disease (CJD), the possible occurrence of variant CJD (vCJD), and potential zoonotic transmission of chronic wasting disease (CWD). Although cross- species transmission of prion diseases seems to be limited by an apparent “species barrier,” the occurrence of bovine spongiform encephalopathy (BSE) and its transmis- sion to humans indicate that animal prion diseases can pose a significant public health risk. Recent reports of secondary person-to-person spread of vCJD via blood products and detection of vCJD transmission in a patient heterozygous at codon 129 further illustrate the potential public health impacts of BSE. INTRODUCTION by IRMO/Information Center on 03/14/05. For personal use only. Prion diseases, also known as transmissible spongiform encephalopathies (TSEs), are a group of animal and human brain diseases that are uniformly fatal and often characterized by a long incubation period and a multifocal neuropathologic picture of neuronal loss, spongiform changes, and astrogliosis (3). -
Ebb and Flow System Versus Overhead Sprinkler and Microirrigation for Container-Grown Woody Ornamental Production in Florida
EBB AND FLOW SYSTEM VERSUS OVERHEAD SPRINKLER AND MICROIRRIGATION FOR CONTAINER-GROWN WOODY ORNAMENTAL PRODUCTION IN FLORIDA By LUIS CARLOS NOGUEIRA A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY UNIVERSITY OF FLORIDA 2005 Copyright 2005 by Luis Carlos Nogueira This dissertation is dedicated to my beloved parents, Luiz Nogueira and Ana Colli Nogueira, who always showed love, patience, understanding and hard work. ACKNOWLEDGMENTS It is always a good time to thank GOD! for the beautiful and powerful nature, full of all resources, for us to work with and learn from. I am very thankful to Dr. Dorota Zofia Haman, a great person and a smart adviser, for the guidance, opportunity, friendship and huge support. Many thanks go to the professors of my committee, Dr. Michael Dukes, Dr. John Schueller, Dr. Robert Stamps and Dr. Thomas Burks, for all the valuable teachings, patience, understanding, and encouragement at all times. I thank them all so much for always telling me to move forward despite the obstacles I faced during my journey here. Also many thanks go to the technician Danny Burch and engineers Larry Miller and Wayne Williams, for lending me their dedicated expertise, patience, and willingness, during all phases of my research. We enjoyed many hours of good times together in lab work, fieldwork, and traveling. I need to express my gratitude to all of my friends, in and out of the University, people with whom I shared good and bad times, reminding me that there are other things in life. -
Innate Immunity Part I: the Immediate Response Deborah A
Innate Immunity Part I: The Immediate Response Deborah A. Lebman, Ph.D. OBJECTIVES 1. Describe three mechanisms present at epithelial surfaces that protect the host from pathogens. 2. List 3 functions of complement proteins in innate immunity 3. Know the primary functions of macrophages and neutrophils 4. List the receptors on macrophages involved in recognition of pathogens 5. List the key toxic products of phagocytes READING • Parham, The Immune System, pg 227-238. TERMS • Defensin: antibacterial peptide produced by the host at epithelial surfaces • Complement: ubiquitously present proteins synthesized primarily in the liver that mediate a variety of responses to pathogens • Opsonin (opsonization): alteration of cell surface that facilitates phagocytosis • Phagocytosis: internalization of particulate matter by cells • CD14: LPS receptor • TLR-4: toll like receptor 4 I. Introduction A. The first response to pathogens occurs immediately B. The early response employs both mechanical and cell mediated mechanisms C. There is no cellular expansion, but there is cell recruitment D. A wide array of soluble mediators with both positive and negative effects, i.e. remove the pathogen but hurt the host, is released II. Overview of Pathogens A. There are four types of pathogen: bacteria, virus, fungus, parasite B. Table I: Examples of the Four Types of Common Human Pathogen Table 1: Examples of the Four Types of Common Human Pathogen Table 1 (Continued): Examples of the Four Types of Common Human Pathogen C. Pathogens can be intracellular or extracellular Table 2: Examples of intracellular and extracellular pathogens. Notice that both innate and adaptive mechanisms are involved in the response to both types of pathogen. -
Growing Hydroponic Leafy Greens Figure 1
FRESH FARE Growing Hydroponic Leafy Greens Figure 1. Lettuce growing in deep Have you thought about making the move toward controlled water culture environment vegetable growing? Here are some things to consider. (DWC) ponds BY NEIL MATTSON eafy greens such as lettuce, arugula, kale, mustard In NFT, seedlings are transplanted onto shallow channels and spinach are among the most popular locally grown where a thin film of nutrient solution is continuously circulated Lvegetables. They can be produced locally year-round (Figure 2). The channels are sloped at 1 to 4 percent away in controlled environment greenhouses in hydroponics. This from a center aisle and drained at the ends back to the water article will cover some of the basics of hydroponic systems and reservoir. An advantage of the DWC system is because a large production methods for these crops. volume of water is used rapid changes are avoided in water temperature, pH, electrical conductivity (EC) and nutrient CHOOSE YOUR SYSTEM solution composition. The two most common hydroponic growing systems for leafy greens are deep water culture (DWC) and nutrient film technique HEAD LETTUCE (NFT). In DWC, also referred to as raft or pond culture, seedlings The Cornell University Controlled Environment Agriculture are transplanted into Styrofoam rafts which are floated on a 6- to (CEA) group has a long history of research to optimize production 12-inch constructed pond containing a large volume of nutrient of hydroponic lettuce. When proper growing conditions are solution (Figure 1). A pump is used to circulate water through maintained a 5- to 6-ounce head of lettuce can be produced from the pond and an air pump or injection with oxygen is used to seed in 35 days. -
*Ponics (Why Google Is Suspects That My Kids Are Drug Dealers and Why We’Re Looking for a Food-Safe Fencepost)
*ponics (Why Google is suspects that my kids are drug dealers and why we’re looking for a food-safe fencepost) Roger Meike (pronounced “Mike,” FYI) PARC - but not what this project is about Saturday, July 20, 13 Roger Meike • Currently working at PARC • Technologist/Maker/Entrepreneur... Dork! • ..and I have the ham radio license to prove it! Saturday, July 20, 13 keyword: spaughts Saturday, July 20, 13 The challenge • Advice from Mike: Pick a topic that interests you • I now work on a software project at PARC, so I don’t have HW to talk about • I’m busy so I don’t do as much fun stuff at home as I would like • Everything I do in hardware these days is more sketchy than a sketch Saturday, July 20, 13 Slow Jam Sketch • If you use really slow material, is it still sketching? • i.e. working with biological and chemical constraints • The story: Why Google is pretty sure my kids are drug dealers and why I’m looking for a food-safe fencepost • Let the children lead the way • And so the story begins... Saturday, July 20, 13 Items of Interest KE6HFO KE6HFP Saturday, July 20, 13 Saturday, July 20, 13 Saturday, July 20, 13 Saturday, July 20, 13 Saturday, July 20, 13 Items of Interest KJ6KEB KI6TGN KE6HFO KE6HFP Saturday, July 20, 13 Saturday, July 20, 13 Hydroponics System Reservoir with pump and float switch Saturday, July 20, 13 Types of Hydroponic Systems • Ebb and flow system o Water level is fluctuated • Wick System o Simplest System • Drip system or Dutch Bucket o Bucket with a drip system type water supply • Deep water culture (DWC) o Roots -
Plant Propagation for Successful Hydroponic Production
2/19/2018 Plant Propagation for Successful Hydroponic Production Hye-Ji Kim Assistant Professor of Sustainable Horticulture Crop Production February 13, 2018 Purdue University is an equal access/equal opportunity institution. What is Hydroponics? Hydroponics = hydros + ponos Water labor The cultivation of plants by placing the roots in liquid nutrient solutions rather than in soil; soilless growth of plants. Purdue University is an equal access/equal opportunity institution. 1 2/19/2018 Why hydroponics? . Crops can be produced on non‐arable land including land with poor soils and/or high salinity levels. Efficient use of water and nutrients. High density planting = minimum use of land area. Year‐round production. Local food. Direct and immediate control over the rhizosphere. Isolation from diseases or insect pests usually found in the soil. Higher yield, quality and storability of products. Ease of cleaning the systems. No weeding or cultivation is needed. Transplanting of seedlings is easy. Purdue University is an equal access/equal opportunity institution. Hydroponics Basics Types of Hydroponics: Water vs. Substrate-base Open vs. Closed Purdue University is an equal access/equal opportunity institution. 2 2/19/2018 Types of Hydroponics: Water vs. Substrate-base Water-based System Substrate-based System Deep water culture “Raft” system Ebb‐and‐flow Nutrient Film Techniques (NFT) Aeroponics Drip irrigation Purdue University is an equal access/equal opportunity institution. Source: Chiwon Lee Types of Hydroponics: Water vs. Substrate-base Water-based System Deep water culture “Raft” system Nutrient Film Techniques (NFT) Aeroponics Source: Chiwon Lee Source: hydrocentre.com.au Source: Petrus Langenhoven Mobile channel system Facility of Great Lakes Growers, Burton, Ohio Purdue University is an equal access/equal opportunity institution. -
Vertical Farming Sustainability and Urban Implications
Master thesis in Sustainable Development 2018/32 Examensarbete i Hållbar utveckling Vertical Farming Sustainability and Urban Implications Daniela Garcia-Caro Briceño DEPARTMENT OF EARTH SCIENCES INSTITUTIONEN FÖR GEOVETENSKAPER Master thesis in Sustainable Development 2018/32 Examensarbete i Hållbar utveckling Vertical Farming Sustainability and Urban Implications Daniela Garcia-Caro Briceño Supervisor: Cecilia Mark-Herbert Evaluator: Daniel Bergquist Copyright © Daniela Garcia-Caro Briceño, Published at Department of Earth Sciences, Uppsala University (www.geo.uu.se), Uppsala, 2018 Content 1. INTRODUCTION .............................................................................................................................. 1 1.1 PROBLEM FORMULATION ............................................................................................................... 1 1.2 AIM ................................................................................................................................................. 2 1.3 OUTLINE ......................................................................................................................................... 3 2. METHODS ......................................................................................................................................... 4 2.1 RESEARCH APPROACH AND DESIGN ............................................................................................... 4 2.2 RESEARCH DELIMITATIONS ........................................................................................................... -
The Pennsylvania State University the Graduate School College Of
The Pennsylvania State University The Graduate School College of Agricultural Sciences GREENTOWERS: PRODUCTION AND FINANCIAL ANALYSES OF URBAN AGRICULTURAL SYSTEMS A Thesis in Horticulture by Jonathan Gumble 2015 Jonathan Gumble Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science August 2015 The thesis of Jonathan Gumble was reviewed and approved* by the following: Robert D. Berghage Associate Professor of Horticulture Thesis Co-Advisor Dan T. Stearns J. Franklin Styer Professor Thesis Co-Advisor Mark A. Gagnon Harbaugh Entrpreneurship Scholar & Entrepreneur Coordinator Andrew Lau Associate Professor of Engineering Rich Marini Professor of Horticulture Head of the Department of Horticulture *Signatures are on file in the Graduate School ii Abstract By the year 2050, the population of planet Earth is expected to reach over nine billion people. In the next 35 years, we will have the task of supporting an additional two billion lives on a planet that is already struggling to provide a stable and acceptable food supply as well as an effective means of food distribution. Estimates show that of the seven billion people living on planet Earth, 870 million suffer from hunger. The fight against world hunger is a complex, challenging, and multi-faceted issue that can only be fought through innovative solutions that address the multiple aspects comprising it. One of these aspects is simply limited access to food in urban neighborhoods and rural towns which are referred to as “food deserts”. These are prevalent throughout the United States and have resulted in food-insecure households. Solutions to limited access do exist today in the form of innovative growing on developed land.