Ticles. the 5,6-Dimethylbenzimidazolylcobamide Coenzyme Has Been Identified in the Azotobacter
1542 MICROBIOLOGY: H. NIKAIDO PROC. N. A. S. supernatant fluid, whereas the cobamide coenzyme remains in the ribosomal par- ticles. The 5,6-dimethylbenzimidazolylcobamide coenzyme has been identified in the azotobacter. We are grateful to David Perlman, the Squibb Institute for Medical Research, New Jersey, for determining vitamin B12 in our preparations and for the gift of cobalt58-labeled hydroxocobala- min. * Sabbatical leave October 1961-1962 at the University of Wisconsin. Aided by grant E-1417 (C6) from the National Institutes of Health and grant C-2826 from the National Science Founda- tion. t Appeared on the program for the Annual Meeting and in the abstracts published in Science as "Metabolism of Inorganic Nitrogen and Its Compounds in Microorganisms" by D. J. D. Nicholas and P. W. Wilson. l Holm-Hansen, 0. G. C., H. Gerloff, and F. Skoog, Physiol. Plantanum, 7, 665 (1954). 2 Shaukat-Ahmed, and H. J. Evans, Biochem. Biophys. Res. Comm., 1, 271 (1959) and Soil Science, 90, 205 (1960). 3 Lowe, R. H., and H. J. Evans, J. Bacteriol., 83, 210 (1962). 4Reisenauer, H. M., Nature, 186, 375 (1960). 5 Hallsworth, E. G., S. B. Wilson, and E. A. N. Greenwood, Nature, 187, 79 (1960). 6 Delwiche, C. C., C. M. Johnson, and H. M. Reisenauer, Plant Physiol., 36, 73 (1961). 7Lowe, R. H., H. J. Evans, and Shaukat-Ahmed, Biochem. Biophys. Res. Comm., 3, 675 (1960). 8 Nicholas, D. J. D., Y. Maruyama, and D. J. Fisher, Biochim. Biophys. Acta, 56, 623 (1962). 9 Hu, A. S. L., R. Epstein, H. 0. Halvorson, and R.
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