Toxigenic Algae and Associated Phycotoxins in Two Coastal
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microorganisms Review The Genetic Basis of Toxin Biosynthesis in Dinoflagellates Arjun Verma 1,* , Abanti Barua 1,2, Rendy Ruvindy 1, Henna Savela 3, Penelope A. Ajani 1 and Shauna A. Murray 1 1 Climate Change Cluster, University of Technology Sydney, Sydney 2007, Australia 2 Department of Microbiology, Noakhali Science and Technology University, Chittagong 3814, Bangladesh 3 Finnish Environment Institute, Marine Research Centre, 00790 Helsinki, Finland * Correspondence: [email protected] Received: 22 June 2019; Accepted: 27 July 2019; Published: 29 July 2019 Abstract: In marine ecosystems, dinoflagellates can become highly abundant and even dominant at times, despite their comparatively slow growth rates. One factor that may play a role in their ecological success is the production of complex secondary metabolite compounds that can have anti-predator, allelopathic, or other toxic effects on marine organisms, and also cause seafood poisoning in humans. Our knowledge about the genes involved in toxin biosynthesis in dinoflagellates is currently limited due to the complex genomic features of these organisms. Most recently, the sequencing of dinoflagellate transcriptomes has provided us with valuable insights into the biosynthesis of polyketide and alkaloid-based toxin molecules in dinoflagellate species. This review synthesizes the recent progress that has been made in understanding the evolution, biosynthetic pathways, and gene regulation in dinoflagellates with the aid of transcriptomic and other molecular genetic tools, and provides a pathway for future studies of dinoflagellates in this exciting omics era. Keywords: dinoflagellates; toxins; transcriptomics; polyketides; alkaloids 1. Introduction Marine microbial eukaryotes are a diverse group of organisms comprising lineages that differ widely in their evolutionary histories, ecological niches, growth requirements, and nutritional strategies [1–5]. -
Influence of Environmental Parameters on Karenia Selliformis Toxin Content in Culture
Cah. Biol. Mar. (2009) 50 : 333-342 Influence of environmental parameters on Karenia selliformis toxin content in culture Amel MEDHIOUB1, Walid MEDHIOUB3,2, Zouher AMZIL2, Manoella SIBAT2, Michèle BARDOUIL2, Idriss BEN NEILA3, Salah MEZGHANI3, Asma HAMZA1 and Patrick LASSUS2 (1) INSTM, Institut National des Sciences et Technologies de la Mer. Laboratoire d'Aquaculture, 28, rue 2 Mars 1934, 2025 Salammbo, Tunisie. E-mail: [email protected] (2) IFREMER, Département Environnement, Microbiologie et Phycotoxines, BP 21105, 44311 Nantes, France. (3) IRVT, Institut de la Recherche Vétérinaire de Tunisie, centre régional de Sfax. Route de l'aéroport, km 1, 3003 Sfax, Tunisie Abstract: Karenia selliformis strain GM94GAB was isolated in 1994 from the north of Sfax, Gabès gulf, Tunisia. This species, which produces gymnodimine (GYM) a cyclic imine, has since been responsible for chronic contamination of Tunisian clams. A study was made by culturing the microalgae on enriched Guillard f/2 medium. The influence of growing conditions on toxin content was studied, examining the effects of (i) different culture volumes (0.25 to 40 litre flasks), (ii) two temperature ranges (17-15°C et 20-21°C) and (iii) two salinities (36 and 44). Chemical analyses were made by mass spectrometry coupled with liquid chromatography (LC-MS/MS). Results showed that (i) the highest growth rate (0.34 ± 0.14 div d-1) was obtained at 20°C and a salinity of 36, (ii) GYM content expressed as pg eq GYM cell-1 increased with culture time. The neurotoxicity of K. selliformis extracts was confirmed by mouse bioassay. This study allowed us to cal- culate the minimal lethal dose (MLD) of gymnodimine (GYM) that kills a mouse, as a function of the number of K. -
Ultrastructure and Molecular Phylogenetic Position of a New Marine Sand-Dwelling Dinoflagellate from British Columbia, Canada: Pseudadenoides Polypyrenoides Sp
European Journal of Phycology ISSN: 0967-0262 (Print) 1469-4433 (Online) Journal homepage: http://www.tandfonline.com/loi/tejp20 Ultrastructure and molecular phylogenetic position of a new marine sand-dwelling dinoflagellate from British Columbia, Canada: Pseudadenoides polypyrenoides sp. nov. (Dinophyceae) Mona Hoppenrath, Naoji Yubuki, Rowena Stern & Brian S. Leander To cite this article: Mona Hoppenrath, Naoji Yubuki, Rowena Stern & Brian S. Leander (2017) Ultrastructure and molecular phylogenetic position of a new marine sand-dwelling dinoflagellate from British Columbia, Canada: Pseudadenoides polypyrenoides sp. nov. (Dinophyceae), European Journal of Phycology, 52:2, 208-224, DOI: 10.1080/09670262.2016.1274788 To link to this article: http://dx.doi.org/10.1080/09670262.2016.1274788 View supplementary material Published online: 03 Mar 2017. Submit your article to this journal Article views: 25 View related articles View Crossmark data Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=tejp20 Download by: [The University of British Columbia] Date: 13 April 2017, At: 11:37 EUROPEAN JOURNAL OF PHYCOLOGY, 2017 VOL. 52, NO. 2, 208–224 http://dx.doi.org/10.1080/09670262.2016.1274788 Ultrastructure and molecular phylogenetic position of a new marine sand-dwelling dinoflagellate from British Columbia, Canada: Pseudadenoides polypyrenoides sp. nov. (Dinophyceae) Mona Hoppenratha,b, Naoji Yubukia,c, Rowena Sterna,d and Brian S. Leandera aDepartments of Botany and Zoology, -
Protocols for Monitoring Harmful Algal Blooms for Sustainable Aquaculture and Coastal Fisheries in Chile (Supplement Data)
Protocols for monitoring Harmful Algal Blooms for sustainable aquaculture and coastal fisheries in Chile (Supplement data) Provided by Kyoko Yarimizu, et al. Table S1. Phytoplankton Naming Dictionary: This dictionary was constructed from the species observed in Chilean coast water in the past combined with the IOC list. Each name was verified with the list provided by IFOP and online dictionaries, AlgaeBase (https://www.algaebase.org/) and WoRMS (http://www.marinespecies.org/). The list is subjected to be updated. Phylum Class Order Family Genus Species Ochrophyta Bacillariophyceae Achnanthales Achnanthaceae Achnanthes Achnanthes longipes Bacillariophyta Coscinodiscophyceae Coscinodiscales Heliopeltaceae Actinoptychus Actinoptychus spp. Dinoflagellata Dinophyceae Gymnodiniales Gymnodiniaceae Akashiwo Akashiwo sanguinea Dinoflagellata Dinophyceae Gymnodiniales Gymnodiniaceae Amphidinium Amphidinium spp. Ochrophyta Bacillariophyceae Naviculales Amphipleuraceae Amphiprora Amphiprora spp. Bacillariophyta Bacillariophyceae Thalassiophysales Catenulaceae Amphora Amphora spp. Cyanobacteria Cyanophyceae Nostocales Aphanizomenonaceae Anabaenopsis Anabaenopsis milleri Cyanobacteria Cyanophyceae Oscillatoriales Coleofasciculaceae Anagnostidinema Anagnostidinema amphibium Anagnostidinema Cyanobacteria Cyanophyceae Oscillatoriales Coleofasciculaceae Anagnostidinema lemmermannii Cyanobacteria Cyanophyceae Oscillatoriales Microcoleaceae Annamia Annamia toxica Cyanobacteria Cyanophyceae Nostocales Aphanizomenonaceae Aphanizomenon Aphanizomenon flos-aquae -
Effects of Marine Harmful Algal Blooms on Bivalve Cellular Immunity and Infectious Diseases: a Review
Effects of marine Harmful Algal Blooms on bivalve cellular immunity and infectious diseases: a review Malwenn Lassudrie, Helene Hegaret, Gary Wikfors, Patricia Mirella da Silva To cite this version: Malwenn Lassudrie, Helene Hegaret, Gary Wikfors, Patricia Mirella da Silva. Effects of marine Harm- ful Algal Blooms on bivalve cellular immunity and infectious diseases: a review. Developmental and Comparative Immunology, Elsevier, 2020, 108, pp.103660. 10.1016/j.dci.2020.103660. hal-02880026 HAL Id: hal-02880026 https://hal.archives-ouvertes.fr/hal-02880026 Submitted on 24 Jun 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Effects of marine Harmful Algal Blooms on bivalve cellular immunity and infectious diseases: a review Malwenn Lassudrie1, Hélène Hégaret2, Gary H. Wikfors3, Patricia Mirella da Silva4 1 Ifremer, LER-BO, F- 29900 Concarneau, France. 2 CNRS, Univ Brest, IRD, Ifremer, LEMAR, F-29280, Plouzané, France. 3 NOAA Fisheries Service, Northeast Fisheries Science Center, Milford, CT 0640 USA. 4 Laboratory of Immunology and Pathology of Invertebrates, Department of Molecular Biology, Federal University of Paraíba (UFPB), Paraíba, Brazil. Abstract Bivalves were long thought to be “symptomless carriers” of marine microalgal toxins to human seafood consumers. -
A Parasite of Marine Rotifers: a New Lineage of Dinokaryotic Dinoflagellates (Dinophyceae)
Hindawi Publishing Corporation Journal of Marine Biology Volume 2015, Article ID 614609, 5 pages http://dx.doi.org/10.1155/2015/614609 Research Article A Parasite of Marine Rotifers: A New Lineage of Dinokaryotic Dinoflagellates (Dinophyceae) Fernando Gómez1 and Alf Skovgaard2 1 Laboratory of Plankton Systems, Oceanographic Institute, University of Sao˜ Paulo, Prac¸a do Oceanografico´ 191, Cidade Universitaria,´ 05508-900 Butanta,˜ SP, Brazil 2Department of Veterinary Disease Biology, University of Copenhagen, Stigbøjlen 7, 1870 Frederiksberg C, Denmark Correspondence should be addressed to Fernando Gomez;´ [email protected] Received 11 July 2015; Accepted 27 August 2015 Academic Editor: Gerardo R. Vasta Copyright © 2015 F. Gomez´ and A. Skovgaard. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Dinoflagellate infections have been reported for different protistan and animal hosts. We report, for the first time, the association between a dinoflagellate parasite and a rotifer host, tentatively Synchaeta sp. (Rotifera), collected from the port of Valencia, NW Mediterranean Sea. The rotifer contained a sporangium with 100–200 thecate dinospores that develop synchronically through palintomic sporogenesis. This undescribed dinoflagellate forms a new and divergent fast-evolved lineage that branches amongthe dinokaryotic dinoflagellates. 1. Introduction form independent lineages with no evident relation to other dinoflagellates [12]. In this study, we describe a new lineage of The alveolates (or Alveolata) are a major lineage of protists an undescribed parasitic dinoflagellate that largely diverged divided into three main phyla: ciliates, apicomplexans, and from other known dinoflagellates. -
Multifunctional Polyketide Synthase Genes Identified by Genomic Survey of the Symbiotic Dinoflagellate, Symbiodinium Minutum
Beedessee et al. BMC Genomics (2015) 16:941 DOI 10.1186/s12864-015-2195-8 RESEARCH ARTICLE Open Access Multifunctional polyketide synthase genes identified by genomic survey of the symbiotic dinoflagellate, Symbiodinium minutum Girish Beedessee1*, Kanako Hisata1, Michael C. Roy2, Noriyuki Satoh1 and Eiichi Shoguchi1* Abstract Background: Dinoflagellates are unicellular marine and freshwater eukaryotes. They possess large nuclear genomes (1.5–245 gigabases) and produce structurally unique and biologically active polyketide secondary metabolites. Although polyketide biosynthesis is well studied in terrestrial and freshwater organisms, only recently have dinoflagellate polyketides been investigated. Transcriptomic analyses have characterized dinoflagellate polyketide synthase genes having single domains. The Genus Symbiodinium, with a comparatively small genome, is a group of major coral symbionts, and the S. minutum nuclear genome has been decoded. Results: The present survey investigated the assembled S. minutum genome and identified 25 candidate polyketide synthase (PKS) genes that encode proteins with mono- and multifunctional domains. Predicted proteins retain functionally important amino acids in the catalytic ketosynthase (KS) domain. Molecular phylogenetic analyses of KS domains form a clade in which S. minutum domains cluster within the protist Type I PKS clade with those of other dinoflagellates and other eukaryotes. Single-domain PKS genes are likely expanded in dinoflagellate lineage. Two PKS genes of bacterial origin are found in the S. minutum genome. Interestingly, the largest enzyme is likely expressed as a hybrid non-ribosomal peptide synthetase-polyketide synthase (NRPS-PKS) assembly of 10,601 amino acids, containing NRPS and PKS modules and a thioesterase (TE) domain. We also found intron-rich genes with the minimal set of catalytic domains needed to produce polyketides. -
Redalyc.A New Record of Azadinium Spinosum (Dinoflagellata) from The
Revista de Biología Marina y Oceanografía ISSN: 0717-3326 [email protected] Universidad de Valparaíso Chile Hernández-Becerril, David U.; Barón-Campis, Sofía A.; Escobar-Morales, Sergio A new record of Azadinium spinosum (Dinoflagellata) from the tropical Mexican Pacific Revista de Biología Marina y Oceanografía, vol. 47, núm. 3, diciembre, 2012, pp. 553-557 Universidad de Valparaíso Viña del Mar, Chile Available in: http://www.redalyc.org/articulo.oa?id=47925145016 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative Revista de Biología Marina y Oceanografía Vol. 47, Nº3: 553-557, diciembre 2012 Research Note A new record of Azadinium spinosum (Dinoflagellata) from the tropical Mexican Pacific Un nuevo registro de Azadinium spinosum (Dinoflagellata) en el Pacífico tropical Mexicano David U. Hernández-Becerril1, Sofía A. Barón-Campis2 and Sergio Escobar-Morales3,4 1Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Apdo. postal 70-305, México, D.F. 04510 México. [email protected] 2Instituto Nacional de Pesca, SAGARPA, Pitágoras 1320, México, D.F. 03310, México 3Posgrado en Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, México D.F., México 4Unidad de Ciencias del Agua, Centro de Investigación Científica de Yucatán (CICY), Cancún, Q.R. 77500, México Abstract.- The species Azadinium spinosum, a small thecate dinoflagellate, originally and recently described from the North Sea, was found for the first time in the tropical Pacific coast of Mexico. -
Toxicity and Nutritional Inadequacy of Karenia Brevis: Synergistic Mechanisms Disrupt Top-Down Grazer Control
Vol. 444: 15–30, 2012 MARINE ECOLOGY PROGRESS SERIES Published January 10 doi: 10.3354/meps09401 Mar Ecol Prog Ser OPEN ACCESS Toxicity and nutritional inadequacy of Karenia brevis: synergistic mechanisms disrupt top-down grazer control Rebecca J. Waggett1,2,*, D. Ransom Hardison1, Patricia A. Tester1 1National Ocean Service, National Oceanic and Atmospheric Administration, 101 Pivers Island Road, Beaufort, North Carolina 28516-9722, USA 2Present address: The University of Tampa, 401 W. Kennedy Blvd., Tampa, Florida 33606, USA ABSTRACT: Zooplankton grazers are capable of influencing food-web dynamics by exerting top- down control over phytoplankton prey populations. Certain toxic or unpalatable algal species have evolved mechanisms to disrupt grazer control, thereby facilitating the formation of massive, monospecific blooms. The harmful algal bloom (HAB)-forming dinoflagellate Karenia brevis has been associated with lethal and sublethal effects on zooplankton that may offer both direct and indirect support of bloom formation and maintenance. Reductions in copepod grazing on K. brevis have been attributed to acute physiological incapacitation and nutritional inadequacy. To evalu- ate the potential toxicity or nutritional inadequacy of K. brevis, food removal and egg production experiments were conducted using the copepod Acartia tonsa and K. brevis strains CCMP 2228, Wilson, and SP-1, characterized using liquid chromatography-mass spectrometry (LC-MS) as hav- ing high, low, and no brevetoxin levels, respectively. Variable grazing rates were found in exper- iments involving mixtures of toxic CCMP 2228 and Wilson strains. However, in experiments with toxic CCMP 2228 and non-toxic SP-1 strains, A. tonsa grazed SP-1 at significantly higher rates than the toxic alternative. -
VII EUROPEAN CONGRESS of PROTISTOLOGY in Partnership with the INTERNATIONAL SOCIETY of PROTISTOLOGISTS (VII ECOP - ISOP Joint Meeting)
See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/283484592 FINAL PROGRAMME AND ABSTRACTS BOOK - VII EUROPEAN CONGRESS OF PROTISTOLOGY in partnership with THE INTERNATIONAL SOCIETY OF PROTISTOLOGISTS (VII ECOP - ISOP Joint Meeting) Conference Paper · September 2015 CITATIONS READS 0 620 1 author: Aurelio Serrano Institute of Plant Biochemistry and Photosynthesis, Joint Center CSIC-Univ. of Seville, Spain 157 PUBLICATIONS 1,824 CITATIONS SEE PROFILE Some of the authors of this publication are also working on these related projects: Use Tetrahymena as a model stress study View project Characterization of true-branching cyanobacteria from geothermal sites and hot springs of Costa Rica View project All content following this page was uploaded by Aurelio Serrano on 04 November 2015. The user has requested enhancement of the downloaded file. VII ECOP - ISOP Joint Meeting / 1 Content VII ECOP - ISOP Joint Meeting ORGANIZING COMMITTEES / 3 WELCOME ADDRESS / 4 CONGRESS USEFUL / 5 INFORMATION SOCIAL PROGRAMME / 12 CITY OF SEVILLE / 14 PROGRAMME OVERVIEW / 18 CONGRESS PROGRAMME / 19 Opening Ceremony / 19 Plenary Lectures / 19 Symposia and Workshops / 20 Special Sessions - Oral Presentations / 35 by PhD Students and Young Postdocts General Oral Sessions / 37 Poster Sessions / 42 ABSTRACTS / 57 Plenary Lectures / 57 Oral Presentations / 66 Posters / 231 AUTHOR INDEX / 423 ACKNOWLEDGMENTS-CREDITS / 429 President of the Organizing Committee Secretary of the Organizing Committee Dr. Aurelio Serrano -
Scrippsiella Trochoidea (F.Stein) A.R.Loebl
MOLECULAR DIVERSITY AND PHYLOGENY OF THE CALCAREOUS DINOPHYTES (THORACOSPHAERACEAE, PERIDINIALES) Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) der Fakultät für Biologie der Ludwig-Maximilians-Universität München zur Begutachtung vorgelegt von Sylvia Söhner München, im Februar 2013 Erster Gutachter: PD Dr. Marc Gottschling Zweiter Gutachter: Prof. Dr. Susanne Renner Tag der mündlichen Prüfung: 06. Juni 2013 “IF THERE IS LIFE ON MARS, IT MAY BE DISAPPOINTINGLY ORDINARY COMPARED TO SOME BIZARRE EARTHLINGS.” Geoff McFadden 1999, NATURE 1 !"#$%&'(&)'*!%*!+! +"!,-"!'-.&/%)$"-"!0'* 111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111 2& ")3*'4$%/5%6%*!+1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111 7! 8,#$0)"!0'*+&9&6"*,+)-08!+ 111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111 :! 5%*%-"$&0*!-'/,)!0'* 11111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111 ;! "#$!%"&'(!)*+&,!-!"#$!'./+,#(0$1$!2! './+,#(0$1$!-!3+*,#+4+).014!1/'!3+4$0&41*!041%%.5.01".+/! 67! './+,#(0$1$!-!/&"*.".+/!1/'!4.5$%"(4$! 68! ./!5+0&%!-!"#$!"#+*10+%,#1$*10$1$! 69! "#+*10+%,#1$*10$1$!-!5+%%.4!1/'!$:"1/"!'.;$*%."(! 6<! 3+4$0&41*!,#(4+)$/(!-!0#144$/)$!1/'!0#1/0$! 6=! 1.3%!+5!"#$!"#$%.%! 62! /0+),++0'* 1111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111111<=! -
Reference Database for the 18S Rrna Gene
Received: 3 November 2017 | Revised: 15 February 2018 | Accepted: 24 February 2018 DOI: 10.1111/1755-0998.12781 RESOURCE ARTICLE DINOREF: A curated dinoflagellate (Dinophyceae) reference database for the 18S rRNA gene Solenn Mordret1 | Roberta Piredda1 | Daniel Vaulot2 | Marina Montresor1 | Wiebe H. C. F. Kooistra1 | Diana Sarno1 1Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Naples, Italy Abstract 2Sorbonne Universite, CNRS, UMR Dinoflagellates are a heterogeneous group of protists present in all aquatic ecosys- Adaptation et Diversite en Milieu Marin, tems where they occupy various ecological niches. They play a major role as primary Station Biologique, Roscoff, France producers, but many species are mixotrophic or heterotrophic. Environmental Correspondence metabarcoding based on high-throughput sequencing is increasingly applied to Diana Sarno, Integrative Marine Ecology, Stazione Zoologica Anton Dohrn, Naples, assess diversity and abundance of planktonic organisms, and reference databases Italy. are definitely needed to taxonomically assign the huge number of sequences. We Email: [email protected] provide an updated 18S rRNA reference database of dinoflagellates: DINOREF. Funding information Sequences were downloaded from GENBANK and filtered based on stringent quality Italian Ministry of Education, University and Research criteria. All sequences were taxonomically curated, classified taking into account classical morphotaxonomic studies and molecular phylogenies, and linked to a series of metadata. DINOREF includes 1,671 sequences representing 149 genera and 422 species. The taxonomic assignation of 468 sequences was revised. The largest num- ber of sequences belongs to Gonyaulacales and Suessiales that include toxic and symbiotic species. DINOREF provides an opportunity to test the level of taxonomic resolution of different 18S barcode markers based on a large number of sequences and species.