DOCSLIB.ORG

Рано Утврђивање Компатибилности Sato-Zakura Трешања И Домаћих Подлога Спајањем Калуса У Условима in Vitro

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Рано Утврђивање Компатибилности Sato-Zakura Трешања И Домаћих Подлога Спајањем Калуса У Условима in Vitro УНИВЕРЗИТЕТ У БЕОГРАДУ ШУМАРСКИ ФАКУЛТЕТ Драгана М. Скочајић РАНО УТВРЂИВАЊЕ КОМПАТИБИЛНОСТИ SATO-ZAKURA ТРЕШАЊА И ДОМАЋИХ ПОДЛОГА СПАЈАЊЕМ КАЛУСА У УСЛОВИМА IN VITRO Докторска дисертација Београд, 2016. UNIVERSITY OF BELGRADE FACULTY OF FORESTRY Dragana M. Skočajić EARLY DETECTION OF THE GRAFT COMPATIBILITY SATO-ZAKURA CHERRYS AND DOMESTIC ROOTSTOCKS BY CALLUS FUSION IN VITRO Doctoral Dissertation Belgrade, 2016. NSZTVRF\N?KTRKSXTVYN]QFSTHNRFPTRNWN?K R R I ,, Y G ^ ] P ) JR 9 ,, Y Gă ^ J9# V ,, N ,, ] JR Z ,F ,, Y Gă U* JJV ,, Y Gă U* J NM?FHKMF[HFQSTWXN YSNHKVMNXKXYGKTIVFJY- ^FVWPNZFPYQXKX @]SFJTPYRKSXF\NTSFNSZTVRF\N?F V )VGG N )NGV X )XJ R ) X XM X ) HHV J ) FFY RJRW ) ,, ) RS R I ,0 Y G^ SS V#+ Sato- zakura +, )+ in vitro ? )?U W 6, * )U V W ) I )IU W ) I +IN 2016. N NM F R +RW ==>?>0VW )0P H = Z ZT) C *0260 0280 a *66 6 6 079 )e, 27 a, 38 66 6 6 0I S ST G S JNW U) U 6P* UT 0JKLJMNO0 *+0 0 )0 . P 635.92.012:582.711.713(043.3) ]] G ^ 0 P H =0==>?>G0 ) HHS S N NM ? ), + VWMO XWYZNW ) ) ) Z ) in vitro0 ) ) Sato-zakura + , : ) ) ) , ) J +U T SS^ 0 01-4170/1027.05.2015. T H, 002 . 61206-2638/2-=]0=^>_`>=] JT P ) PT R I ,00^ 0 JR 9 ,0 ^ 9#V ,0 N ,] JR Z ,F ,0 U* JJV ,00U* UNIVERSITY OF BELGRADE - FACULTY OF FORESTRY KEY WORDS DOCUMENTATION Accession number (ANO) Identification number (INO) Document type (DT) Monographic publication Type of record (TR) Textual printed document Contains code (CC) Doctoral Dissertation Author (AU) DraganaM. VYO6WJ40Msc. of Forestry for Landscape architecture and horticulture Mentor (MN) Dr JWJONJ4, Full Professor Title (TI) Early detection of the graft compatibility Sato- zakura cherrys and domestic rootstocks by callus fusion under in vitro Language of text (LT) Serbian/ Cyrillic alphabet Country of publication (CP) Republic of Serbia Locality of publication (LP) Serbia Publication year (PY) 2016 Publisher AuthorΤs reprint Publication place (PL) 11030 Belgrade, R. Serbia, Kneza Višeslava 1 Physical description (PD) (number of 8 chapters, 260 pages, 280 references, 79 figures, chapters/pages/citations/tables/charts/maps/ 27 tables, 38 charts, 4 maps images/annexes) Science field (SF) Biotechnological sciences Science discipline (SD) Landscape architecture and horticukture Subject/Key words (CX) in vitro, incompatibility, grafting, callus, fusion, union, phenols UDC Holding data(HD) Library of Faculty of Forestry, Kneza Višeslava 1, 11030 Belgrade, R. Serbia Note (N) None Abstract (AB) Japanese flowering cherry trees from the group Sato zakura are of great importance for landscape architecture and horticulture. The early detection of the compatibility of the aforementioned scions and rootstocks was being carried out by fusing the calluses of the scion and rootstock in vitro: The unions were obtained by visual and histochemical union analyses (the cell arrangement and the cell wall staining as well as the presence of lipids and phenol components in the callus). Accepted by scientific board on (ACB) Decision of Academic-Scientific Council of Faculty of Forestry, No. 01-4170/1, from 27.05.2015. Decision of Professional Board of Biotechnical Sciences, 02 No. 61206-2638/2-15, from 17.06.2015. Defended on (DE) Thesis defend board (DB) Dr. JWJONJ4, full professor Faculty of Forestry, Belgrade Dr. WMJW@ZYJ4, full professor Faculty of Forestry, Belgrade Dr. @ZN7JKWZJ4, Principal Research Fellow Fruit Research Intitute05W6WY Dr. JJ¡W¢OMJNJ4£YJ4, associate professor Faculty of Agriculture, Belgrade ¥N¥NW¦WKWWK6J40W§§J§MWKM¨NO©ª§§ON Faculty of Agriculture, Belgrade РАНО УТВРЂИВАЊЕ КОМПАТИБИЛНОСТИ SATO-ZAKURA ТРЕШАЊА И ДОМАЋИХ ПОДЛОГА СПАЈАЊЕМ КАЛУСА У УСЛОВИМА IN VITRO Резиме Јапанске цветајуће трешње из групе Sato zakura имају посебан значај за пејзажну архитектуру и хортикултуру широм света па и код нас. Како се већина добија путем хетеровегетативног размножавања, поље истраживања је компатибилност најчешће гајених култивара украсних трешања на нашем поднебљу: Prunus serrulata ʼAmanogawaʼ, Prunus serrulata ʼKanzanʼ, Prunus serrulata ʼKiku-shidare-zakuraʼ, са домаћим подлогама за трешању: Prunus avium, Prunus ʼColtʼ и Prunus mahaleb. Таксон који је коришћен у истраживању као позитивна (компатибилна) контрола је Prunus serrulata, а два потенцијално инкомпатибилна таксона - Prunus serotina и Pyrus communis ʼPyrodwarfʼ. Фузијом калуса подлоге и племке у условима in vitro, на релативно брз и поуздан начин вршена је рана детекција компатибилности поменутих племки и подлога. Резултати о јачини и компатибилности спојева добијени су визуелним (проценат успешно спојених, степен јачине споја) и хистохемијским анализама споја (распоред и обојеност ћелијских зидова и присуство липида и фенолних компоненти у калусу). Од три коришћене хранљиве подлоге (MS, SH и WP), за највећи број таксона, најпогоднија у процесу индукције калуса се показала МS. За обичну трешњу и култиваре Prunus serrulata ʼKanzanʼ и Prunus ʼColtʼ, у процесу индукције калуса из пупољака или вегетативних делова летораста, одговарајућа је била и SH подлога, док се једино за врсту Prunus mahaleb, WP издвојила као погодна хранљива подлога. Са ауксином NAA у концентрацији 2 mgL-1 и цитокинином BAP 0,5 mgL-1 за већину таксона добијени су најбољи резулатати у калогенези. За генотипове Prunus avium, Prunus ʼColtʼ, Prunus serotina и Prunus serrulata ʼKanzanʼ, могуће је користити у истој концентрацији и 2,4-D као замену, док се IBA показао неодговарајућим ауксином за индукцију калуса код истраживаних врста. На спојевима делова добијеног калуса подлоге и племке, визуелно оцењена јачина споја показала је јаке спојеве код комбинација: P. avium/ P. serrulata ʼKanzanʼ, Prunus ʼColtʼ/P. serrulata ʼAmanogawaʼ, Prunus ʼColtʼ/P. serrulata ʼKiku-shidare-zakuraʼ и P. serrulata са сва три култивара. Делимично јаки спојеви остварени су код калуса P. avium/P. serrulata ʼAmanogawaʼ и ʼKiku-shidare-zakuraʼ, Prunus ʼColtʼ/ P. serrulata ʼKanzanʼ и код спојева сва три култивара са касном сремзом (Prunus serotina). Јасне знаке неподударности калуса кроз лако одвајање на споју, показале су, очекивано, комбинације сва три култивара јапанских украсних трешања са култиваром Pyrus communis ʼPyrodwarfʼ и са магривом (Prunus mahaleb). i Анатомске анализе су указале на значајне манифестације у ткиву спојева калуса: бојењем рутенијум црвеном бојом локализоване су веће количине пектинских полисахарида код потенцијално компатибилних спојева, насупрот мањој локализацији ових једињења код инкомпатибилних. Бојењем толуидин и алцијан плавом и сафранином, доста прецизно су локализовани феноли, као и сенесценција ткива, што може да укаже на ране знаке неподударности калуса. Поменуте појаве јасно су забележене код спојева култивара P. serrulata са магривом и патуљастом крушком (ʼPyrodwarfʼ) и местимично на спојевима са култиваром ʼColtʼ и са обичном трешњом (P. avium/P. serrulata ʼAmanogawaʼ и P. avium/P. serrulata ʼKiku-shidare-zakuraʼ) и код спојева са касном сремзом. Хемијским анализама спојева, потврђена је компатибилност из претходне две анализе као и постојање одређеног броја неподударних спојева. Детекција неких полифенола који су показатељи инкопмпатибилности калемљења (p - кумаринска киселина, кемферол, кверцетин, ферулинска киселина, нарингенин и баикалеин), потврдила је на још један, посредан начин, потенцијално инкомпатибилне спојеве. Код магриве, хемијским анализама укупних и појединачних полифенола потврђено је њихово нагомилавање јасно манифестовано потамњивањем ткива, а на спојевима са обичном трешњом (која се код нас најчешће користи као подлога за калемљење јапанских украсних трешања), хемијске анализе су потврдиле постојање одређених сметњи у споју ове врсте са култиварима ʼAmanogawaʼ и ʼKiku-shidare-zakuraʼ. Према свим анализама за ова два култивара боље резултате су дале подлога Prunus serrulata и Prunus ʼColtʼ. Основни таксон Prunus serrulata могао би се користити као алтернативна подлога и/или посредник калемљења између наших подлога и јапанских украсних трешања, а Prunus ʼColtʼ као алтернатива за домаћу трешњу као подлогу, чиме би се превазишла неподударност између поменутих компонената калемљења. Сумирањем резултата спроведених истраживања, може се констатовати да се примењена метода фузије калуса у in vitro условима може сматрати ефикасним начином добијања релатвно брзог одговора о потенцијалној подударности две компоненте калемљења и да је метода применљива и за друге таксоне. Применом ове технике могли би се, макар у одређеном обиму, избећи пољски огледи у којима се одговор о подударности калемљења може чекати више година. Кључне речи: трешња, Prunus serrulata, инкомпатибилност, in vitro, калемљење, калус, спој, подлога, племка, феноли ii EARLY DETECTION OF THE COMPATIBILITY OF SATO-ZAKURA CHERRY TREES AND DOMESTIC ROOTSTOCKS BY FUSING CALLUSES IN VITRO Abstract Japanese flowering cherry trees from the group Sato zakura are of great importance for landscape architecture and horticulture both worldwide and in our country. As the majority are reproduced via heterovegetative propagation, the field of research is the compatibility of the most frequently cultivated ornamental cherry trees cultivars in our region: Prunus serrulata 'Amanogawa', Prunus serrulata 'Kanzan', Prunus serrulata 'Kiku-shidare-zakura', with domestic cherry rootstocks: Prunus avium, Prunus 'Colt' and Prunus mahaleb. The taxon that was used in the research as a positive (compatible) control is Prunus serrulata,
Load more

Recommended publications
  • Cloning and Bioinformatics Analysis of Rosa Rugosa S Locus F-Box Gene (Rrslf)
    American Journal of Plant Sciences, 2017, 8, 1550-1559 http://www.scirp.org/journal/ajps ISSN Online: 2158-2750 ISSN Print: 2158-2742 Cloning and Bioinformatics Analysis of Rosa rugosa S Locus F-Box Gene (RrSLF) Yuyuan Wei1,2*, Kang Li1,2*, Shutang Xing3*, Dandan Zhao1,2, Changli Fu1,2, Lanyong Zhao1,2, Dekui Zang1,2, Xiaoyan Yu1,2# 1Forestry College of Shandong Agricultural University, Tai’an, China 2Shandong Provincial Research Center of Landscape Engineering Technology for Urban and Rural, Tai’an, China 3College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China How to cite this paper: Wei, Y.Y., Li, K., Abstract Xing, S.T., Zhao, D.D., Fu, C.L., Zhao, L.Y., Zang, D.K. and Yu, X.Y. (2017) Cloning In order to reveal the phenomenon of R. rugosa pollination incompatibility, and Bioinformatics Analysis of Rosa rugosa the full-length cDNA sequence of S Locus F-box Gene was cloned for the first S Locus F-Box Gene (RrSLF). American Journal of Plant Sciences, 8, 1550-1559. time from the pollen of Rosa rugosa “Zilong wochi” with RT-PCR and RACE https://doi.org/10.4236/ajps.2017.87107 methods and named as RrSLF. The full-length cDNA is 1236 bp with an open reading frame of 1122 bp, encoding 343 amino acids. The derived protein has Received: March 27, 2017 Accepted: June 13, 2017 a molecular weight of 43.7 kD, a calculated pI of 6.24, an F-box conserved Published: June 16, 2017 domain at position 343 - 741, and belongs to F-box family.
    [Show full text]
  • Official Journal of the European Communities No L 100/35
    17 . 4 . 80 Official Journal of the European Communities No L 100/35 COUNCIL DIRECTIVE of 18 March 1980 amending the Annexes to Directive 77/93/EEC on protective measures against the introduction into the Member States of organisms harmful to plants or plant products (80/393 / EEC) THE COUNCIL OF THE EUROPEAN Whereas any risk should be prevented which may COMMUNITIES , arise from the introduction on potato breeding material , of harmful organisms unknown in the Having regard to the Treaty establishing the Euro­ Community ; whereas, therefore, the protective pean Economic Community , measures laid down in this connection in Annex III (A) should be extended ; Having regard to Council Directive 77 /93 /EEC of 21 December 1976 on protective measures against Whereas the measures laid down in the said Direc­ the introduction into the Member States of organ­ tive in the case of oak wood in the round , against isms harmful to plants or plant products ( J ), and in the introduction of oak wilt (Ceratocystis faga­ particular Article 13 thereof, cearum) into the Community have on the one hand proved insufficientand , on the other unnecessarily Having regard to the prosposal from the Commis­ strict ; whereas therefore , the ' special requirements ' sion , established in respect of this disease (Annex IV (A) (2)) should take into account the technical safe­ Whereas, in the interests of ^ uniform implementa­ guards which have been recognized since then ; tion of Directive 77/93/EEC the term 'harmful viruses and mycoplasmas' of fruit plants (in parti­
    [Show full text]
  • (Ph.D.) Értekezés a CSERESZNYE S-LÓKUSZÁNAK VARIABILITÁSA A
    Doktori (Ph.D.) értekezés A CSERESZNYE S-LÓKUSZÁNAK VARIABILITÁSA A GÉNCENTRUMBAN Szikriszt Bernadett Kertészettudományi Doktori Iskola Budapesti Corvinus Egyetem Genetika és Növénynemesítés Tanszék Budapest 2012 A Doktori Iskola Megnevezése: Kertészettudományi Doktori Iskola Tudományága: Növénytermesztési és kertészeti tudományok Vezetője: Dr. Tóth Magdolna egyetemi tanár, D.Sc. Budapesti Corvinus Egyetem, Kertészettudományi Kar, Gyümölcstermő Növények Tanszék Témavezetők: Dr. Halász Júlia egyetemi docens, Ph.D. Dr. Hegedűs Attila Egyetemi docens, Ph.D. Budapesti Corvinus Egyetem, Kertészettudományi Kar, Genetika és NövénynemesítésTanszék A jelölt a Budapesti Corvinus Egyetem Doktori Szabályzatában előírt valamennyi feltételnek eleget tett, az értekezés műhelyvitájában elhangzott észrevételeket és javaslatokat az értekezés átdolgozásakor figyelembe vette, ezért az értekezés nyilvános védési eljárásra bocsátható. .................................................. .................................................. Dr. Halász Júlia Dr. Tóth Magdolna .................................................. Dr. Hegedűs Attila A doktori iskola vezetőjének jóváhagyása A témavezetők jóváhagyása 2 A Budapesti Corvinus Egyetem Élettudományi Területi Doktori Tanács 2012. évi október 2.-i határozatában a nyilvános vita lefolytatására az alábbi bíráló Bizottságot jelölte ki: BÍRÁLÓ BIZOTTSÁG: Elnöke: Tóth Magdolna, D.Sc. Tagjai: Palkovics László, D.Sc. Deák Tamás, Ph. D. Szani Zsolt, Ph.D. Janda Tibor, D.Sc. Opponensek: Farkas Ágnes, Ph.D. Bisztray György
    [Show full text]
  • Characterization of the S-Rnase Genomic DNA Allele Sequence in Prunus Speciosa and P
    Scientia Horticulturae 144 (2012) 93–101 Contents lists available at SciVerse ScienceDirect Scientia Horticulturae journa l homepage: www.elsevier.com/locate/scihorti Characterization of the S-RNase genomic DNA allele sequence in Prunus speciosa and P. pseudocerasus a,b a,∗ a a a a a,∗∗ Chao Gu , Jun Wu , Shu-Jun Zhang , Ya-Nan Yang , Hua-Qing Wu , Shu-Tian Tao , Shao-Ling Zhang a College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, People’s Republic of China b Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, People’s Republic of China a r t i c l e i n f o a b s t r a c t Article history: In this study, eight S-RNase alleles were isolated from two Prunus pseudocerasus and two Prunus speciosa Received 15 February 2012 accessions by PCR amplification from genomic DNA. Analysis of the amino acid sequences revealed five Received in revised form 27 June 2012 novel and three published S-alleles. These S-RNases share typical structural features with S-RNases from Accepted 29 June 2012 other Prunus species exhibiting gametophytic self-incompatibility. The deduced amino acid identities ranged from 60.8 to 75.6% among four S-RNase alleles in Prunus speciosa and ranged from 73 to 81.4% Keyword: among four S-RNase alleles in Prunus pseudocerasus. The size of the first introns ranged from 197 to Prunus speciosa 341 bp, and the size of second introns ranged from 81 to 1182 bp. Sequence analysis demonstrated that Prunus pseudocerasus Self-incompatibility the deduced amino acid identities, by comparison with other Prunus species, were often higher than S-RNase those of intraspecific identities.
    [Show full text]
  • Erenoğlu Danişmanlik Tercümanlik Ve Diş Ticaret
    ANNEX –1 HARMFUL ORGANISMS THAT ARE SUBJECT TO QUARANTINE AND THAT HINDER IMPORTATION A-HARMFUL ORGANISMS NOT KNOWN TO OCCUR IN TURKEY, THAT ARE SUBJECT TO QUARANTINE AND THAT HINDER IMPORTATION Insects Acleris gloverana Acleris variana Aeolesthes sarta Agrilus auroguttatus Agrilus anxius Agrilus planipennis Aleurolobus marlatti Amauromyza maculosa Anastrepha fraterculus Anastrepha ludens Anastrepha obliqua Anastrepha suspensa Anoplophora glabripennis Anoplophora malasiaca Anthonomus bisignifer Anthonomus eugenii Anthonomus grandis Anthonomus quadrigibbus Anthonomus signatus Apriona cinerea Apriona germari Apriona japonica Aromia bungii Arrhenodes minutus 11Bactericera cockerelli Bactrocera ciliatus Bactrocera cucumis Bactrocera cucurbitae Bactrocera latifrons Bactrocera minax Bactrocera dorsalis Bactrocera tryoni Bactrocera tsuneonis Bactrocera zonatus Blitopertha orientalis Cacyreus marshalli 1Carneocephala fulgida Ceratitis rosa Choristoneura spp. Conotrachelus nenuphar Cydia inopinata Cydia packardi 1 Dendroctonus adjunctus Dendroctonus brevicomis Dendroctonus frontalis Dendroctonus ponderosae Dendroctonus pseudotsugae Dendroctonus rufipennis Dendrolimus sibiricus Diabrotica balteata Diabrotica barberi Diabrotica speciosa Diabrotica trivittata Diabrotica undecimpunctata howardi Diabrotica undecimpunctata undecimpunctata Diabrotica virgifera zeae 2Diaphorina citri Diabrotica virgifera 2Diaphorina citri Diaprepes abbreviatus 1Draeculacephala minerva Drosophila suzukii Dryocoetes confusus Epichoristodes acerbella Epitrix cucumeris Epitrix
    [Show full text]
  • List by Latin Genus/Species Names and English Common Names Latin Genus/Species Name English Common Name
    List by Latin Genus/Species Names and English Common Names Latin Genus/Species Name English Common Name Abelmoschus Esclentus Okra Abies Alba European Silver Fir Silver Fir White Fir Abies Balsamea American Silver Fir Balm of Gilead Balsam Canada Balsam Fir Eastern Fir Abies Koreana Korean Fir Abies Pectinata Silver Fir Abies Sibirica Siberian Fir Abronia Villosa Desert Sand-verbena Acacia Arabica Babul Acacia Egyptian Acacia Indian Gum-arabic-tree Scented-thorn Thorn-mimosa Thorny Acacia Acacia Catechu Black Cutch Catechu Acacia Concinna Soap-pod Acacia Dealbata Mimosa Silver Wattle Acacia Decurrens Green Wattle Acacia Farnesiana Cassie Huisache Opopanax Popinac Sweet Acacia Acacia Mearnsii Black Wattle Tan Wattle Acacia Senegal Gum-arabic Kher Senegal-gum Acacia Seyal Shittimwood Talh Thirtythorn Whistlingtree Acacia Victoriae Bramble Acacia Bramble Wattle Acanthopanax Koreanum No common names identified Acanthopanax Senticosus Eleuthero Eleuthero Ginseng Siberian Ginseng Stachelpanax Acer Palmatum Japanese Maple Acer Pseudoplatinus Sycamore Maple Acer Saccharum Sugar Maple Achillea Millefolium Milfoil Yarrow Achras Sapota Wild Dilly Wild Sapodilla Achyranthes Bidentata Nui Xi Achyranthes Fauriei Hinata-ino-kuzuchi Achyrocline Satureiodes Macela Acmella Oleracea Para-cress Toothacheplant Acorus Calamus Acorus Calamus Myrtle Flag Sweet Calamus Sweet Flag Acorus Gramineus Grass-leaf Calamus Grass-leaf Sweetflag Acronychia Acidula Lemon Aspen Acronychia Pedunculata Cavi Jejerukan Actinidia Arguta Bower Actinidia Taravine Vine-pear Actinidia
    [Show full text]
  • Village of Round Lake Tree and Landscape Manual
    Village of Round Lake Tree and Landscape Manual TREES AND LANDSCAPING IN THE VILLAGE OF ROUND LAKE ARE AN IMPORTANT AND VALUABLE COMMUNITY RESOURCE. THE PROTECTION AND ENHANCEMENT OF THIS RESOURCE IS A HIGH PRIORITY. THIS MANUAL HAS BEEN PREPARED AT THE DIRECTION OF THE VILLAGE BOARD OF TRUSTEES. THESE REQUIREMENTS AND PROCEDURES ESTABLISH A COMPREHENSIVE PROCESS FOR THE DEPARTMENT OF PUBLIC WORKS TO FOLLOW IN THE FULFILLMENT OF ITS ROLE IN THE ADMINISTRATION OF THE TREE PRESERVATION, STREET TREE AND LANDSCAPE ORDINANCES OF THE VILLAGE OF ROUND LAKE. CONTENTS Page 1. TREE PRESERVATION 3- 31 A. Requirements 1. Subdivision Development a. inventory b. plan format c. tree preservation plan requirements d. site preparations – pre-construction 2. Individual Lots a. lot development plans b. lot redevelopment and lot improvement plans c. lot demolition only 3. Ad hoc tree removal not related to lot development, or lot improvement B. Tree Preservation Standards 1. Subdivision 2. Individual Lots II. STREET TREES 32-37 A. Planting Standards and Specifications 1. Adopted Standards 2. Inspection of Material 3. Quality of Plant Material 4. Rejection of Plant Material 5. Delivery Village of Round Lake Administrative Manual 1-25-06 Page 1 of 87 6. Planting 7. Spacing B. Tree Planting Detail C. Permitted Species List D. Prohibited Species List III. LANDSCAPING AND SCREENING 38-50 A. Landscape Design Criteria B. Concept Design Plan C. Final Landscape Plan D. Selection and Maintenance of Plant Material E. Parking Lot Landscaping F. Perimeter Landscaping (Residential) G. Foundation Landscaping H. Perimeter Landscaping (Non-Residential & Multi-Family) I. Non-Residential Property Abutting Residential Property J.
    [Show full text]
  • Shiseido Future Solution Lx Universal Defense E
    SHISEIDO FUTURE SOLUTION LX UNIVERSAL DEFENSE E- octisalate, octocrylene, and zinc oxide cream SHISEIDO AMERICAS CORPORATION Disclaimer: Most OTC drugs are not reviewed and approved by FDA, however they may be marketed if they comply with applicable regulations and policies. FDA has not evaluated whether this product complies. ---------- SHISEIDO FUTURE SOLUTION LX UNIVERSAL DEFENSE E Drug Facts Active ingredients Purpose Octisalate 5.0% Sunscreen Octocrylene 7.0% Sunscreen Zinc Oxide 15.4% Sunscreen Uses helps prevent sunburn if used as directed with other sun protection measures (see Directions), decreases the risk of skin cancer and early skin aging caused by the sun Warnings For external use only Do not use on damaged or broken skin When using this product keep out of eyes. Rinse with water to remove. Stop use and ask a doctor if rash occurs. Keep out of reach of children. If product is swallowed, get medical help or contact a Poison Control Center right away. Flammable. Do not use near heat, flame or while smoking. Directions For sunscreen use: apply liberally 15 minutes before sun exposure reapply: after 80 minutes of swimming or sweating immediately after towel drying at least every two hours Sun Protection Measures. Spending time in the sun increases your risk of skin cancer and early skin aging. To decrease this risk, regularly use a sunscreen with a broad spectrum SPF of 15 or higher and other sun protection measures including: limit time in the sun, especially from 10 a.m. – 2 p.m. wear long-sleeve shirts, pants, hats, and sunglasses
    [Show full text]
  • The Lectotypification of Prunus Jamasakura and Allied Native
    Bull. Natl. Mus. Nat. Sci., Ser. B, 45(4), pp. 147–164, November 22, 2019 The Lectotypification of Prunus jamasakura and Allied Native Species of Cerasus sect. Sargentiella in Japan (Rosaceae—Prunoideae) Hideaki Ohba1 and Shinobu Akiyama2,* 1 Department of Botany, the University Museum, the University of Tokyo, 7–3–1 Hongo, Tokyo 113–0033, Japan 2 Department of Botany, National Museum of Nature and Science, 4–1–1 Amakubo, Tsukuba, Ibaraki 305–0005, Japan *E-mail: [email protected] (Received 20 August 2019; accepted 25 September 2019) Abstract The names of Prunus fimbriisepala Nakai, P. fimbriisepala Nakai var. grandiflora Nakai, P. idzuensis Nakai, P. jamasakura Siebold ex Koidz., P. jamasakura Siebold ex Koidz. [unranked] α. elegans Koidz. [unranked] c. compta Koidz., P. jamasakura Siebold ex Koidz. [unranked] β. speciosa Koidz., P. occultans Nakai, P. pseudocersus Lindl. var. β. borealis Makino, P. pseudocerasus Lindl. [var.] α. spontanea Maxim., P. pseudocerasus Lindl. var. α. jamasakura (Siebold) Makino subvar. b. pubescens Makino, P. pseudocerasus Lind. [var.] α. spontanea Maxim. subvar. humilis Makino, P. rotundipetala Nakai, P. verecunda (Koidz.) Koehne var. inter- media Nakai are lectotypified. Two new combinations, Cerasus jamasakura Siebold ex Koidz. f. pendula (Sugim.) H.Ohba & S.Akiyama and C. jamasakura Siebold ex Koidz. f. purpureorubra (T. Kawasaki) H.Ohba & S.Akiyama, are proposed. Nomenclatorial notes to explain the lectotypi- fication are presented. Key words: Cerasus, Jamasakura, Japanese cherry, lectotypification, Prunus, Yama-zakura. The Japanese native species of cherry, genus the species concerned here and recognized two Cerasus sect. Sargentiella (sensu Kawasaki, varieties, α. floribus simplicibus ... and β. floribus 1991) were studied by many workers since pre- plenis maximis albis ..
    [Show full text]
  • 'Nanosuit' Successfully Protects Petals of Cherry Blossoms in High Vacuum
    www.nature.com/scientificreports OPEN A ‘NanoSuit’ successfully protects petals of cherry blossoms in high vacuum: examination of living Received: 1 November 2017 Accepted: 10 January 2018 plants in an FE-SEM Published: xx xx xxxx Sayuri Takehara1, Yasuharu Takaku1, Hiroshi Suzuki2, Isao Ohta3, Masatsugu Shimomura4 & Takahiko Hariyama1 Land plants have evolved on dry land and developed surface barriers to protect themselves from environmental stresses. We have previously reported that polymerization of a natural extracellular substance (ECS) on the outer surface of animals by electron beam or plasma irradiation, can give rise to a nano-scale layer, termed the “NanoSuit”, which can keep small animals alive under the high vacuum of a scanning electron microscope (SEM). In the present research, we have focused on plants, using petals of cherry blossoms, as experimental specimens and examined their behavior under high vacuum conditions. Experiments on healthy living petals have demonstrated that without any pre- treatment, the overall morphology of specimens is well preserved and intact after imaging in an SEM, suggesting that natural substances on the petal surface behave like animal ECS and form a NanoSuit following irradiation with an electron beam. Furthermore, we have shown that the surface material can be extracted with chloroform and polymerized into a free-standing membrane by plasma irradiation. From our results, we conclude that surface materials, which have the ability to prevent water loss under natural conditions, increase the barrier ability and can protect plants under high vacuum conditions. Land plants have evolved on dry land and developed surface barriers to protect themselves from desiccation, UV exposure, high irradiation, and other abiotic environmental stresses1–3.
    [Show full text]
  • CLE DE PEAU BEAUTE SMOOTHING BASE for LINES- Titanium Dioxide Cream SHISEIDO CO., LTD
    CLE DE PEAU BEAUTE SMOOTHING BASE FOR LINES- titanium dioxide cream SHISEIDO CO., LTD. Disclaimer: Most OTC drugs are not reviewed and approved by FDA, however they may be marketed if they comply with applicable regulations and policies. FDA has not evaluated whether this product complies. ---------- CLE DE PEAU BEAUTE SMOOTHING BASE FOR LINES Drug Facts Active ingredient TITANIUM DIOXIDE 9.9% Purpose Sunscreen Uses helps prevent sunburn if used as directed with other sun protection measures (see Directions), decreases the risk of skin cancer and early skin aging caused by the sun Warnings For external use only Do not use on damaged or broken skin When using this product keep out of eyes. Rinse with water to remove. Stop use and ask a doctor if rash occurs Keep out of reach of children. If product is swallowed, get medical help or contact a Poison Control Center right away. Directions For sunscreen use: apply liberally 15 minutes before sun exposure use a water resistant sunscreen if swimming or sweating reapply at least every 2 hours Sun Protection Measures. Spending time in the sun increases your risk of skin cancer and early skin aging. To decrease this risk, regularly use a sunscreen with a broad spectrum SPF of 15 or higher and other sun protection measures including: limit time in the sun, especially from 10 a.m. – 2 p.m. wear long-sleeve shirts, pants, hats, and sunglasses children under 6 months: Ask a doctor Inactive Ingredients WATER • CYCLOMETHICONE • GLYCERIN • DIMETHICONE • POLYMETHYL METHACRYLATE • DIPROPYLENE GLYCOL • PEG-10
    [Show full text]
  • Szilvafajták Jellemzése Ssr- És S- Lókusz Markerekkel
    SZILVAFAJTÁK JELLEMZÉSE SSR- ÉS S- LÓKUSZ MARKEREKKEL Doktori (PhD) értekezés MAKOVICSNÉ ZSOHÁR NOÉMI TÉMAVEZETŐ: DR. HALÁSZ JÚLIA habilitált egyetemi docens, Ph.D. BUDAPEST 2019 A doktori iskola megnevezése: Kertészettudományi Doktori Iskola Tudományága: Kertészeti biológia Vezetője: Zámboriné dr. Németh Éva egyetemi tanár, DSc tanszékvezető, Gyógy- és Aromanövények Tanszék Szent István Egyetem, Kertészettudományi Kar Témavezető: Dr. Halász Júlia habilitált egyetemi docens, Ph.D. Genetika és Növénynemesítés Tanszék Szent István Egyetem, Kertészettudományi Kar A jelölt a Szent István Egyetem Doktori Szabályzatában előírt valamennyi feltételnek eleget tett, az értekezés műhely vitájában elhangzott észrevételeket és javaslatokat az értekezés átdolgozásakor figyelembe vette, azért az értekezés védési eljárásra bocsátható. .................................................... .................................................... Zámboriné dr. Németh Éva Dr. Halász Júlia Iskolavezető Témavezető 2 Az az anya, aki gyermeke szívébe a természet szeretetét beojtotta, mesés kincsek birodalmával ajándékozta meg szülöttét; olyan kincseket ad annak, melyeket az idő vasfoga sem emészthet meg; melyek azt boldogítva kisérendik végig az élet utain. Hazám lelkes leányai! Ajándékozzátok meg gyermekeiteket eme kincsekkel, a természet szent szeretetének boldogító érzésével! Karoljátok fel hazai gyümölcsészetünk ügyét! Szeressétek, ápoljátok a gyümölcsészetet! A gyümölcsészet egy áldásdús tudomány, olyan mint a szeretet vallása, mely nem egyes népeket hanem az
    [Show full text]