Bio-Data and Current Research Work of Zahoor Ahmed

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BIO-DATA AND CURRENT RESEARCH WORK OF ZAHOOR AHMED

NAME ZAHOOR AHMED

DESIGNATION ASSOCIATE PROFESSOR

PERMANENT ADDRESS Forensic Science Laboratory, School of Biological Sciences, Quaid-i- Azam Campus, University of the Punjab, Lahore, Pakistan.

MARITAL STATUS Married: Wife: Naseem, Children: Noreen, Farrah, Sehar

PASSPORT NUMBER CN1150601 Booklet Number A1714764

Date of Birth April 13, 1952 E – mail [email protected] [email protected]

EDUCATIONAL QUALIFICATIONS:

University of Agriculture 1977 M.Sc. (Botany) Faisalabad.

University of the Punjab, 1990 Ph.D. (Molecular Lahore. Biology)

TRAINING and RESEARCH:

1975-1978 Research for Masters thesis on "Effect of Chlorocoline Chloride (CCC) on Protein. Carbohydrates yield of Wheat" and Post M.Sc research at the effect of CCC on the Salt tolerance of Wheat.

1979-1982 Doctoral research on "Isolation, Purification and Characterization of thymine glycol endonuclease from E. coli with Dr. S. Riazuddin at NIAB, Faisalabad, Pakistan. This was one part of doctoral research.

1982- Research for doctoral thesis on "Enzymatic repair of O-alkylated thymidine residues in DNA" with Prof. J. Laval at Institute Gustave Roussy, Villejuif, Paris, France. This was second part of doctoral research. 2 -

1983- Training on the purification of restriction endonucleases from different microorganisms, in Dr. R. J. Roberts Lab., at Cold Spring Harbor Labs., NY. USA.

1983-1985 Research on "The Purification of Restriction Endonucleases from different bacterial strains" at the Centre for Advanced Molecular Biology, University of the Punjab, Lahore, Pakistan.

1986-1987 Post Doctoral research on "The genetic manipulation of hydrocarbon biodegradation" with Prof. S. Riazuddin at Centre for Advanced Molecular Biology, University of the Punjab, Lahore.

1988-1989 Post Doctoral research in Dr. Marck A. Schell's Lab. University of Georgia, Athens, Georgia, USA.

1990-1991 Post Doctoral research on "The degradation of 2.4-Dichlorophenol and Trichloroethylene by transgenic tobacco and poplar plants, respectively". With Prof. Milton P. Gordon, Department of Biochemistry, University of Washington, Seattle, USA.

AWARDS

1982-1983 Research fellowship from Association for Research on Cancer, (France) in the Lab. of Prof. Laval, Institute Gustave Roussy, Paris.

1988-1989 Research fellowship from USAID to conduct postdoctoral research project in the lab. of Dr. Marck A. Schell, University of Georgia, Athens, USA.

2002- Most Productive Scientist By Ministry of Science & Technology

INTERNATIONAL STANDING

2004- 2007 Member Ad-Hoc Standing committee, East Mediterranean Regional Network for Human Genomics and Biotechnology established at Tehran by EMRO, WHO

TRAINING COURSES ATTENDED

1981- Two week course on "Genetic Engineering and Recombinant DNA" held at Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.

1986- A five week course on "Recombinant DNA Techniques" at the Centre for Advanced Molecular Biology, jointly organized by CAMB 3 -

and NYU Medical Centre, USA, and Max Plank Institut Zuchtungs forschung, Koln, Germany

1986- A two week training course on "Protein Techniques" at Centre for Advanced Molecular Biology, Jointly organized by CAMB and Hatfield Polytechnic , U.K.

1987- A six week training course on "Introduction to Industrial Biotechnology", organized by GBF, Braunschweig, Germany.

1991- A one month course on “Safety protection in the use of Radioactivity and Radiation”, Department of Environmental health, University of Washington, Seattle, USA.

1997- “BIOINDUSTRY”, a three month course to visit and survey Japanese bioindustry, sponsored by JICA, Japan.

SUGGESTED REFERENCES:

1. Dr. J. Laval, Biochimie, Institute Gustave Roussy 39 a 53, Rue Camille Desmoulins-94800 Villejuif, (Val-de-Marne-1), France. T. No. 726-4658-643 .

2. Dr. James Hamby Director, Indianapolis—Marion County Forensic Services Agency 40 S Alabama Street Indianapolis IN 46204 USA 4 -

RESEARCH EXPERIENCE:

I started my research career in 1975. When I joined Dr. Feroza Baig's laboratory for my M.Sc thesis. During stay in her laboratory I worked on various aspects of effects of Chlorocholine Chloride (CCC, a synthetic growth regulator) on the growth of wheat. This work involved the use of different biochemical techniques commonly employed in research on the biochemistry of protein and DNA enzymes. After my M.Sc thesis in 1979. I joined Dr. Riazuddin's laboratory in Faisalabad to work for my Ph.D thesis on the enzymology of DNA repair. As part of my Ph.D thesis project, I spent one year in Dr. J. Laval's laboratory at Institute Gustave Roussy, Paris and four months in Dr. R. J. Roberts's laboratory at Cold Spring Harbor Laboratories, New York, USA. My training in Villejuif and New York exposed me to experiences involving the use of a number of molecular biological techniques commonly used in recombinant DNA research.

In 1983, when Dr. Riazuddin moved to Lahore to establish a new laboratory for advanced molecular biology at the University of the Punjab, I moved with him to Lahore and initiated a new programme of research on the biodegradation of long chain hydrocarbons. As a result of input into this programme, 60 bacterial isolates from different ecological environments in Pakistan have been collected, taxonomically identified and their potential to degrade aliphatic/aromatic hydrocarbons are being studied.

In 1988, I proceeded to USA for a postdoctoral in Dr. Mark A. Schell's laboratory at UGA, Athens, Georgia, USA under financial support from USAID. During stay in Mark Schell's lab., I studied the expression and excretion of Polyglucoturonase (PGase) of Pseudomonas solanacerum. Different sequences of PGase were deleted with Ba131 to study the role of different sequences in excretion. PGase deletions were fused with alkaline phosphatase or catechol 2.3-oxygenase as reporter gene to detect translocation of deleted PGase into different compartments of E. coli and Pseudomonas. These fusions were constructed into pUC9 and excretion was studied in E. coli JM 107. The fusions were then subcloned into pRK404 (a mobilizable vector). E. coli JM 107 was transformed with these constructions and then conjugated with Pseudomonas solanacerum's PGase to determine the effect of deletions on the excretion.

In 1990, I went to Seattle to study molecular biology of degradation of 2,4- Dichlorophenol and Trichloroethylene in plants in Milton P. Gordon,s laboratory. Transgenic tobacco plants were made by Agrobacterium mediated transformation of N. Tabaccum (xanthi) with a Ti vector containing two genes (2,4-DCP Hydroxylase and Catechol 2,3-dioxygenase) for the degradation of 2,4-Dichlorophenol. N. gluaca plants were also transformed with the same constructs. The degradation of 2,4-DCP and TCE was also studied in Poplar plants. Since TCE can contaminate aquifers, therefore, long root system of Poplar can present a remedy to clean the contaminated sites. All these studies were conducted in hydroponic conditions.

In 1995, a project was started to study the synthesis of stress proteins induced by xenobiotics (toxic chemicals) in E. coli. The objective is to identify specific pollutants by identifying stress proteins induced by those toxic chemicals. The ultimate aim is to develop ELISA and dip-stick tests for the identification of toxic chemicals. The stress proteins are identified and purified with two-dimensional polyacrylamide gel 5 - electrophoresis. The waste of synthetic plastic poses threats to our ecosystem as well as human health. The concept of biodegradable plastic produced by microbes or plants has become a hot area for research. In 1997, production of biological polymer from bacteria was started. A number of Pseudomonas and Bacillus species were isolated which produces poly hydroxyl butyric acid (PHB) polymers. The composition is analyzed by GC and IR spectroscopy. Large-scale isolation of PHB is in process.

A program was launched to upgrade the R&D activities of the Centre. Upgradation contains the capacity building programme for commercial production of value added products i.e. a) production of industrial enzymes, b) production of disease free potato seed and c) increase the number of M.Phil./ Ph.D. students to meet the demand of growing biotechnology industry.

With 24 years experience of DNA analysis, a new concept of forensic DNA genotyping of evidence materials of criminal cases has been initiated after validation of SOPs. The infrastructure established at CAMB is comparable to any good laboratory worldwide. After validation, SOP’s have been implemented for casework. Similarly, cutting edge technology of single nucleotide polymorphism, y-chromosome analysis and mitochondrial DNA analysis has been initiated. These state of the art technologies are believed to improve criminal identification tremendously in future.

The development of DNA database of convicted offenders has been initiated. This database will help law and order agencies in tracing the criminals and eventually reduction in crime rate is anticipated. Presently, my group of forensic science is constituted of, fivePh.D. students. Presently, involved in the development of multiplex of Y- Chromosome STR‘s which will help not only the identification of sexual offenders as well as population migration of the past. Genotyping is also being validated for the analysis of trisomy or other chromosomal abnormalities. Initiative have been made to develop a discipline of Forensic Science and Molecular Biology at University of the Punjab.

Planning and Developmental Experience

Construction of CAMB phase- 1 initiated in 1983 when I moved from NIAB to University of the Punjab, Lahore.. The labs were renovated, equipments were purchased and installed , first lab of restriction enzyme purification was made functional. The students were trained to initiate other projects of DNA repair enzymology, environmental biotechnology etc.

Construction of CAMB phase-2 was initiated in 1987 when CAMB was upgraded to Centre of Excellence. The labs were planned according to the requirements of different sections and a unique set-up of centralized utilities was introduced .The equipments were purchased according to the needs of the experimental needs of different sections.

State of the Art Containment Laboratories: Designed state of the art containment laboratories for virology research. This design has eliminated some of the commercially inherited defects in the designs of standards of clean class laboratories.

Development of National Forensic Science Agency: At the occasion of first out of six training workshops for senior and investigation officers of Police, a 6 - recommendation was forwarded to the Ministry of Interior that a National Forensic Science Agency may be developed in order to conduct forensic analysis of crime cases under one roof where modern technologies may be applied for the analysis of evidence materials of criminal cases. Presently collaborating with the National Forensic Science Agency for the development of their laboratories and training of human resource.

Development of Discipline of Advanced Forensic Sciences : This discipline is proposed to be developed at the university campus of University of the Punjab, Lahore. This discipline will cater the needs of police and other law enforcement / investigation agencies in fighting crimes and terrorism. This discipline will also develop data bases of convicted offenders/ suspects / detainees, animals of economical importance as well as plants of agricultural, medicinal and industrial importance. These applications of modern forensic science will generate peripheral economic activities.

Development of East Mediterranean Human Genomic and Biotechnology Network ( EMHGBN ): I was selected as member of ad hoc steering committee of East Mediterranean regional organization of WHO in 2004 at Tehran meeting. It was decided to develop this network in Tehran and also decided to develop national networks in each member country of EMRO. I have been selected to work as focal person in order to develop national human genomic and biotechnology network.

Commercial Ventures

DNA Analysis for Crime Investigation ( analyzed more than 100 criminal cases )

DNA Analysis for Parentage Investigation

PCR Based Diagnosis of Infectious Diseases

Prenatal Analysis of Trisomy and Other Chromosomal Abnormalities by Genotyping

GRANT SUPPORTS:

ARC, Repair of O- alkylated DNA bases by E. coli. FRANCE FFr 28000, executed in France.

USAID Cloning of catabolic genes in E.coli and Pseudomonas US $ 21500, executed in USA.

TWAS: Biodegradation of Naphthalene by Pseudomonas putida US$ 3000, executed in Pakistan.

NSRD: Cloning expression of naphthalene degradation genes in E. coli and Pseudomonas putida. Rs.375,000, executed in Pakistan.

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PSF: Biodegradation of Dichlorophenol (DCP) and Trichloroethylene (TCE) by tobacco. Rs,382,143, executed in Pakistan.

MoST Development of Environmental Diagnostic Kit Successfully completed

Development of Biopesticidal Formulation, Successfully completed

Upgradation of CAMB Laboratories, Completed

DNA Analysis for Crime Investigation Completed Successfully

PROFESSIONAL POSTS HELD:

1983 - 1985 Research Officer/Lecturer, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore.

1985 - 1997 Assistant Professor, Centre of Excellence in Molecular Biology University of the Punjab, Lahore.

1990 - 1991 Visiting Assistant Professor, Department of Biochemistry, University of Washington. Seattle, Washington, USA.

1997 – 2005 Associate Professor, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore

2005 – 2006 Associate Professor Convener, Research complex& Centre for Advanced Forensic Sciences,

CHAIRMAN, Department of Biology, Allama Iqbal Open University Islamabad

2006 - Associate Professor Forensic Science Laboratories, School of Biological Sciences, University of the Punjab Quaid – I – Azam Campus Lahore 8 -

Membership of Committees Held

1986 -- 2004 Chairman, Institutional Purchase Committee, CEMB University of the Punjab JAN. -- 2001 Member, delegation of Pakistan, participated in first SAARC Technical Committee meeting on Science and Technology. New Delhi, Jan. 23-25, 2001.

2004 -- 2005 Member, Technical / Professional Selection Committee University of the Punjab 2004 – 2005 Member, Research committee, University of the Punjab

2005 – 2006 Convener, Research Complex Committee, Allama Iqbal Open University, Islamabad 2005-- 2006 Chairman, Central Purchase Committee, Allama Iqbal Open University, Islamabad

M. Phil. Thesis Guided

Adil Mahmood Homology between naturally occurring degradative plasmids of pseudomonas species and NAH-7.

Shakira Manzoor Degradation of trichloroethylene in plants.

Sajida Rashid Biodegradation of the TCE and DCP by tobacco plants.

Saleem-ud-Din Co-metabolism of pentachlorophenol and trichloroethylene by tobacco plants.

Amir Jalal Uptake of chromium by water hyacinth.

M. Sabir Hussain Biodegradation of pentachlorophenol & tricholorothylene.

Zaib Sarwar Biodegradion of industrial polutants (phenol)& N-Phnol) by micro-organismes.

Tanvir Hussain Degradation of M-Xylen and Toluene by Water Hyacinth.

Muhammad Ferhan Biodegradation of TCE and Phenol by Oxidizing Bacteria

Sarfraz Ahmad Nawaz 2-D PAGE Analysis of Xenobiotic Induced Proteins of E. coli.

M. Nadeem Asghar 2-D PAGE analysis of pesticide induced stress proteins of E. coli

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M. Phil. Thesis Guided In 2002-2003

M. Saqib Shahzad Development of STR Multiplex System (FGA, D3S1358. and Sex Typing Marker Amelogenin) for Forensic Case Work.

Yasir Abbas Bokhari Development of STR Multiplex System (D5S818, D7S820 and D18S51) for Forensic Case Work.

Mian Obaidullah Studies on Y-STR Loci in Arain Population of Pakistan.

Dr. M. Faraz Arshad Malik Studies on Y-STR Loci in Awan Population of Pakistan.

M. Shafique Studies on Y-STR Loci in Rajpoot Population of Pakistan.

Beenish Bashir Mutation analysis of insulin gene in Pakistani Population.

Shahid Mahmood chohan Identification of Markers, looking at the Plasma Proteome of different stages of development of diabetes type-II

Haiba Kaul Comparison of Plasma protein profiles of patients with insulin dependent diabetes Melitus (IDDM).

M. Phil. Thesis Guided In 2003-2004

Alla Rakha Molecular Analysis of Association Between Spermatogenetic Problems and Y – STR’s

Qaiser M. Khan Haplotype Analysis of 20 Y- STR Markers For Forensic Case Work and Population Study

Shahla Nargis Mir DNA Investigation and Human Identification Using Single Nucleotide Polymorphism For Forensic Case Work

Gul Naz Shabir The Essence of Single Nucleotide Polymorphism ( SNP’s ) For Human Identity Testing And Forensic Purpose

Majid Bashir Y – Chromosome STR Analysis For Forensic Case Work And Population Study

Ph.D. Thesis Guided upto-2004 at CEMB Fareeha Zulfiqar

Aleena Samrin

Sajida Mir

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PUBLICATIONS

Research Articles • Ahmed, Z., F. Baig and N. A. Baig (1980). The effect of CCC on protein carbohydrate and yield of wheat. Pak. J. Sci. Res.42. • Ahmed, Z. and J. Laval (1984). Enzymatic repair of O-alkylated thymidine residues in DNA: Involvement of A 04-methylthymine DNA methyl transferase and 02-methylthymine glycosylase, BBRC, 120: 1-8. • Athar A. Ahmed, Z. and Riazuddin, S., (1984). Adaptive response of Mircococcus luteus to alkylating chemicalls. Nucleic Acid Research 12: 2111- 2126. • Riazuddin, S., Athar, A., Ahmed, Z., Lali, S. M. and Sohail, A. (1987). DNA glycosylase enzymes induced during chemical adaptation of M. luteus. Nucleic Acid Res. 15: (16) 6607-6624 • Riazuddin, S., and Ahmed, Z., (1987). A non-pyrimidine dimer UV-damage specific endonculease. Pak. J. Zool. 19: 160-175. • Riazuddin, S., Athar, A., Sohail, A. and Ahmed, Z. (1987). Molecular mechanism of host controlled restriction and modifiction in Haemophilus influenzae. Pak. J. Zool. 19(4): 393-405. • Riazuddin, S., and Ahmed, Z., (1988) selective production of thymine - thymine and cytosine-cytosine dimers. Pak. J. Pharm.1(1): 1 - 8. • Zahoor Ahmed, Saleem ud Din, Milton, P. Gordon and S. Riazuddin (1998) Metabolism of Trichloroethylene and Dichloroethane by Poplar plants (Populus deltoides). Pak. J. Biochem. & Mol. Biol. 2: 51-56. • Zahoor Ahmed, Zaib Sarwar, Nadeem Ahmed, Muhammad Sabir Hussain and S. Riazuddin (1998) Genetic Modification of Pseudomonas putida for Enhanced Degradation of Phenol and Nitrophenol. Pak. J. Biochem. & Mol. Biol. 2: 32- 37. • Ahmed, Z.M., Riazuddin, S., Bernstein, S.L., Ahmad, Z., Khan, Shaneen, Griffith, A.J., Morell, R.J., Friedman, T.B., Riazuddin, S. and Wilcox, E.R. (2001). Mutations of Protocadherin Gene PCDH15 Cause Usher Syndrome Type 1F. Am. J. Hum. Genet. 69(1): 25-34. • Ben-Yousef, T, Wattenhofer, M., Riazuddin, S., Ahmed, Z.M., Scott, H.S., Kudoh, J., Shibuya, K., Antonarakis, S.E., Bonne-Tamir, B., Radhakrishna, U., Naz, S., Ahmad, Z., Riazuddin, S., Pandya, A., Nance, W.E., Wilcox, R.R., Friedman, T.B. and Morell, R.J. (2001). Novel mutations of TMPRSS3 in four DFNB8/B10 families segregating congenital autosomal recessive deafness. J. Med. Genet. 38(6): 396-403. • Bork, J.M., Peters, L.M., Riazuddin, S., Bemstein, S.L., Ahmed, Z.M., Ness, S.L., Polomeno, R., Ramesh, A., Schloss, M., Srikumari Srisailpathy, C.R., Wayne, S., Bellman, S., Desmukh, D. and Ahmed, Z. (2001). Usher syndrome 1D (USHID) 11 -

and nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of the novel cadherin-like CDH23. Am. J. Hum. Genet. 68: 26-37 • Bokhari, S.Y.A., Shehzad,M.S., Qayyum,A., Raza, M.H., Arshad, F., Shafique, M., Rehman, Z., Shahid, A.A., Ahmed, Z and Riazuddin, S. ( 2003 ) Development of STR Multiplex ( D5S818, D7S820, D18S51 ) For Forensic Case Work. Proc. Pak. Congr. Zool. 23: 167 – 174 • Bashir B., Shehzad, M.S. Bokhari, S.Y.A., Raza, M.H., Ahmed, N., Ahmed, Z. and Riazuddin, S. ( 2003 ) Analysis of insulin gene in patients with type 1 diabetes mellitus for mutations. Proc. Pak. Congr. Zool. 23: 191 – 198 • Zubair M. Ahmed , Xiaoyan Cindy Li, Shontell D. Powell, Saima Riazuddin, Terry-Lynn Young, Khushnooda Ramzan, Zahoor Ahmed, Sandra Luscombe, Kiran Dhillon, Linda MacLaren, Barbara Ploplis, Lawrence I Shotland, Elizabeth Ives, Sheikh Riazuddin, Thomas B. Friedman, Robert J. Morell, Edward R. Wilcox ( 2004 ) Characterization of a full Length TMPRSS3 isoform and identification of mutant alleles responsible for nonsyndromic recessive deafness in Newfoundland and Pakistan. BMC Medical Genetics 5: 1 – 8. • . Zhang, Q., Zulfiqar, F., Riazuddin, S.A., Xiao, X., Ahmad, Z., Riazuddin, S., Hejtmancik, J.F. ( 2004 ) Autosomal recessive retinitis pigmentosa in a Pakistani family mapped to CNGA1 with identification of a novel mutation. Mol. Vis. 10: 884-9. • M. Saqib Shahzad, S. Yassir Abbas, Abdul Qayyum Rao, M. Hashim Raza, ObaidUllah, Zia ur Rehman, A. Ali Shahid, Zahoor Ahmed, and S. Riazuddin (2004). Population Studies of STR Loci (D3S1358, D5S818, D7S820, D18S51 and FGA) in NWFP and Sindhi Populations of Pakistan for Forensic Use. Arch. Med. Sadowej Kryminol 54(4): 215 – 22 • S. Amer Riazuddin, Afshan Yasmeen, Qingjiong Zhang, Wenliang Yao, Muhammad Farooq Sabar, Zahoor Ahmed, Sheikh Riazuddin and J. Fielding Hejtmancik, ( 2005 ) A New Locus for Autosomal Recessive Nuclear Cataract Mapped to Chromosome 19q13 in a Pakistani Family. Investigative Opthalmology and Visual Science, 46 : 623 - 626 • Asghar, M.N., Ashfaq, M. Ahmed, Z Khan, I,U. (2006) 2-D PAGE analysis of pesticide-induced stress proteins of E. coli. Anal Bioanal. Chem. 384: 946- 950

• Faraz Malik, Mahmood A. Kayani, M. Ansar, Obaid Ullah, Muhammad Shafeeq,Shahid Chohan, Yasir Abbas, Saqib Shahzad, Ali Raza, Rahat Rehman, Faizan Raiz, Muhammad Hassan siddiqi, Allah Rakha, Zia ur Rehman and Zahoor Ahmed ( 2008 ) Developmentof 19-plex Y STR systemand polymorphism studiesin Pakistani population. ACAD J. XJTU 20 (4) 267 - 273

Symposium Proceedings 12 -

• Riazuddin, S., Athar, A. and Ahmed, Z. (1982). Repair of alkylation damages in M. luteus. "Proceeding of Int'I. Meeting on Inducible Repair. Tolouse, France. • Ahmed, Z. (1997) Application of Pulse Field in Two Dimensional Gel Electrophoresis. In Proceedings of “Application of Genetic Engineering in the fields of Agriculture, Health and Environment”. Held in November, 1997, Lahore, Pakistan. • Zahoor Ahmed, Nadeem Ahmed, Sarfraz Ahmed Nawaz and S. Riazuddin (2000) xenobiotic induced stress protein in E.coli. Proceeding of third International Biennial Conference of Pakistan Society for Microbiology March 28-30, 2000, Lahore. • Shahnaz Chaudhry, Zahoor Ahmed, Imran Waheed and S. Riazuddin (2000) Isolation of Biodegradable Plastics from Bacteria. Proceedings of Third International Biennial conference of Pakistan Society for Microbiology, March 28-30, 2000, Lahore. • Shahzad, M.S., Bokhari, S.Y.A., Hashim, M., Qayyum, A., Ahmad, Z., Rahman, Z. and S. Riazuddin (2003). Development of STR Multiplex System (FGA, D3S1358 and Sex Typing Marker Amelogenin) for Forensic Case Work. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.89 • Bokhari, S.Y.A., Shahzad, M.S., Hashim, M., Qayyum, A., Ahmad, Z., Rahman, Z. and S. Riazuddin (2003). Development of STR Multiplex System ( D5S818, D7S820 and D18S51) for Forensic Case Work. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.90 • Khan, M.A., Ahmad, Z., Qadeer, M.A., Hussain M. and S. Riazuddin.(2003). Isolation of Bacillus sp. From local habitat for Production of Alkaline Protease. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.52 • Sumrin, A., Ahmad, Z. and S. Riazuddin (2003). Modification of Bacillus Strain to Enhance Production of Alpha-Amylase. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.60

Articles in Preparation • Zahoor Ahmed, Nadeem Ahmed, Sarfraz Ahmed, Nawaz Muhammed Nadeem Asghar. 2-D PAGE Analysis of Metal induced proteins of E.Coli. J. Bacteriol. (in preparation) • Zahoor Ahmed, Muhammed Nadeem Asghar, Nadeem Ahmed, Sarfraz Ahmed Nawaz. Two dimentional PAGE Analysis of pesticides induced proteins of E.Coli. (in preparation for .J.Bacteriol ) • Zahoor Ahmed, Shahnaz chaudhry, Rahat Abdul Rehman, Synthesis of biodegrable polymer by Bacillus and pseudomasspp. J. Bacteriol. (in preparation) 13 -

RESEARCH Articles OF LAST FIVE YEARS

Research Articles • Bokhari, S.Y.A., Shehzad,M.S., Qayyum,A., Raza, M.H., Arshad, F., Shafique, M., Rehman, Z., Shahid, A.A., Ahmed, Z and Riazuddin, S. ( 2003 ) Development of STR Multiplex ( D5S818, D7S820, D18S51 ) For Forensic Case Work. Proc. Pak. Congr. Zool. 23: 167 – 174 • Bashir B., Shehzad, M.S. Bokhari, S.Y.A., Raza, M.H., Ahmed, N., Ahmed, Z. and Riazuddin, S. ( 2003 ) Analysis of insulin gene in patients with type 1 diabetes mellitus for mutations. Proc. Pak. Congr. Zool. 23: 191 – 19. • Zubair M. Ahmed , Xiaoyan Cindy Li, Shontell D. Powell, Saima Riazuddin, Terry-Lynn Young, Khushnooda Ramzan, Zahoor Ahmed, Sandra Luscombe, Kiran Dhillon, Linda MacLaren, Barbara Ploplis, Lawrence I Shotland, Elizabeth Ives, Sheikh Riazuddin, Thomas B. Friedman, Robert J. Morell, Edward R. Wilcox ( 2004 ) Characterization of a full Length TMPRSS3 isoform and identification of mutant alleles responsible for nonsyndromic recessive deafness in Newfoundland and Pakistan. BMC Medical Genetics 5: 1 – 8. • Zhang, Q., Zulfiqar, F., Riazuddin, S.A., Xiao, X., Ahmad, Z., Riazuddin, S., Hejtmancik, J.F. ( 2004 ) Autosomal recessive retinitis pigmentosa in a Pakistani family mapped to CNGA1 with identification of a novel mutation. Mol. Vis. 10: 884-9. • M. Saqib Shahzad, S. Yassir Abbas, Abdul Qayyum Rao, M. Hashim Raza, ObaidUllah, Zia ur Rehman, A. Ali Shahid, Zahoor Ahmed, and S. Riazuddin (2004). Population Studies of STR Loci (D3S1358, D5S818, D7S820, D18S51 and FGA) in NWFP and Sindhi Populations of Pakistan for Forensic Use. Arch. Med. Sadowej Kryminol 54(4): 215 – 22 • S. Amer Riazuddin, Afshan Yasmeen, Qingjiong Zhang, Wenliang Yao, Muhammad Farooq Sabar, Zahoor Ahmed, Sheikh Riazuddin and J. Fielding Hejtmancik, ( 2005 ) A New Locus for Autosomal Recessive Nuclear Cataract Mapped to Chromosome 19q13 in a Pakistani Family. Investigative Opthalmology and Visual Science, 46 : 623 - 626 • Asghar, M.N.Ashfaq, M. Ahmed, Z Khan, I,U. (2006) 2-D PAGE analysis of pesticide-induced stress proteins of E. coli. Anal Bioanal. Chem. 384: 946-95

• Qingjiong Zhang · Fareeha Zulfiqar · Xueshan Xiao · S. Amer Riazuddin, Zahoor Ahmad · Raphael Caruso · Ian MacDonald · Paul Sieving, Sheikh Riazuddin · J. Fielding Hejtmancik ( 2007 ) Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene. Human Genetics, DOI 10.1007/s00439-007-0395- 2 : • Faraz Malik, Mahmood A. Kayani, M. Ansar, Obaid Ullah, Muhammad Shafeeq,Shahid Chohan, Yasir Abbas, Saqib Shahzad, Ali Raza, Rahat Rehman, Faizan Raiz, Muhammad Hassan siddiqi, Allah Rakha, Zia ur Rehman and Zahoor Ahmed ( 2008 ) 14 -

Developmentof 19-plex Y STR system and polymorphism studies in Pakistani population. ACAD J. XJTU 20 (4) 267 - 273 •

Symposium Proceedings • Zahoor Ahmed, Nadeem Ahmed, Sarfraz Ahmed Nawaz and S. Riazuddin (2000) xenobiotic induced stress protein in E.coli. Proceeding of third International Biennial Conference of Pakistan Society for Microbiology March 28-30, 2000, Lahore. • Shahnaz Chaudhry, Zahoor Ahmed, Imran Waheed and S. Riazuddin (2000) Isolation of Biodegradable Plastics from Bacteria. Proceedings of Third International Biennial conference of Pakistan Society for Microbiology, March 28-30, 2000, Lahore. • Shahzad, M.S., Bokhari, S.Y.A., Hashim, M., Qayyum, A., Ahmad, Z., Rahman, Z. and S. Riazuddin (2003). Development of STR Multiplex System (FGA, D3S1358 and Sex Typing Marker Amelogenin) for Forensic Case Work. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.89 • Bokhari, S.Y.A., Shahzad, M.S., Hashim, M., Qayyum, A., Ahmad, Z., Rahman, Z. and S. Riazuddin (2003). Development of STR Multiplex System ( D5S818, D7S820 and D18S51) for Forensic Case Work. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.90 • Khan, M.A., Ahmad, Z., Qadeer, M.A., Hussain M. and S. Riazuddin.(2003). Isolation of Bacillus sp. From local habitat for Production of Alkaline Protease. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.52 • Sumrin, A., Ahmad, Z. and S. Riazuddin (2003). Modification of Bacillus Strain to Enhance Production of Alpha-Amylase. Proceedings from the 7th International Conference on Trends In Biochemistry And Molecular Biology (April 2-5 2003). pp.60

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JOURNAL IMPACT FACTOR OF Dr. ZAHOOR AHMED Co authors Year Title of Res. Paper Name of Impact Vol. Page Journal Factor

Ahmed, Z., F. Baig 1980 The effect of CCC on Pak. J. Sci. 42. and N. A. Baig protein carbohydrate Res and yield of wheat. Ahmed, Z. and 1984 Enzymatic repair of Biochemical 3.00 120 1-8. J. Laval O-alkylated thymidine Biophysical residues in DNA: Research Involvement of A Commun. 04-methylthymine DN Methyl transferase and 02-methylthymine glycosylase,

Athar A. Ahmed, Z 1984 Adaptive response of Nucleic Acid 7.552 12 2111-26 and Riazuddin, S Mircococcus luteus to Research alkylating chemicals

Riazuddin, S., 1987 DNA glycosylase Nucleic Acid 7.552 15 6607-24 Athar, A., Ahmed, enzymes induced during Research Z., Lali, S. M. chemical adaptation of and Sohail, A M. luteus. Riazuddin, S., and Ahmed, Z., 1987 A non-pyrimidine dimmer Pakistan 19 160-75 UV-damage specific Journal endonculease. of Zoology Riazuddin, S., 1987 Molecular mechanism of Pakistan 19 393-405 Athar, A., Sohail, A. host controlled restriction Journal 16 - and Ahmed, Z. and modifiction in of Zoology . Haemophilus influenzae.

Riazuddin, S., 1988 selective production Pakistan 1 1-8 and Ahmed, Z., of thymine – thymine Journal of and cytosine-cytosine Pharmacy dimers Zahoor Ahmed, 1998 Metabolism of Pakistan 2 51-56 Saleem ud Din, Trichloroethylene and Journal of Milton, P. Gordon. Dichloroethane by Biochemistry and S. Riazuddin Poplar plants & Molecular (Populus deltoides). Biology Zahoor Ahmed, 1998 Genetic Modification of Pakistan 2 32-37 Zaib Sarwar, Pseudomonas putida Journal of Nadeem Ahmed, for Enhanced Degradation Biochemistry Muhammad Sabir of Phenol and & Molecular Hussain and Nitrophenol. Biology S. Riazuddin Ahmed, Z.M., 2001 Mutations of American 12.649 69 25-34 Riazuddin, S., Protocadherin Gene Journal of Bernstein, S.L., PCDH15 Cause Human Ahmad, Z., . Usher Syndrome Genetics Khan, Shaneen, Type 1F. Griffith, A.J., Morell, R.J., Friedman, T.B., Riazuddin, S. and Wilcox, E.R.

Ben-Yousef, T, 2001 Novel mutations of Journal of 4.330 38 396-403 Wattenhofer, M., TMPRSS3 in four Medical Riazuddin, S., DFNB8/B10 families Genetics Ahmed, Z.M., segregating congenital Scott, H.S., autosomal recessive 17 -

Kudoh, J., deafness. Shibuya, K., Antonarakis, S.E., Bonne-Tamir, B., Radhakrishna, U., Naz, S., Ahmad, Z., Riazuddin, S., Pandya, A., Nance, W.E., Wilcox, R.R., Friedman, T.B. and Morell, R.J.

Bork, J.M., 2001 Usher syndrome American 12.649 68 26-37 Peters, L.M., 1D (USHID) and Journal of Riazuddin, S., nonsyndromic autosomal Human Bemstein, S.L., recessive deafness Genetics Ahmed, Z.M., DFNB12 are caused Ness, S.L., by allelic mutations Polomeno, R., of the novel Ramesh, A., cadherin-like CDH23. Schloss, M., Srikumari Srisailpathy, C.R., Wayne, S., Bellman, S., Desmukh, D. and Ahmed, Z. Bokhari, S.Y.A., 2003 Development of STR Proc. Pak. 23: 167-74 Shehzad,M.S., Multiplex System Congr. Zool Qayyum,A., ( D5S818, D7S820 Raza, M.H., D18S51 ) for Forensic Arshad, F., Case Work Shafique, M., Rehman, Z., Rehman, Z., 18 -

Ahmed, Z and Riazuddin, S.

Bashir B., 2003 Analysis of insulin Proc. Pak. 23: 191-98 Shehzad, M.S. gene in patients Congr. Zool Bokhari, S.Y.A., with type 1 diabetes Raza, M.H., mellitus for mutations Ahmed, N., Ahmed, Z. and Riazuddin, S

Zubair M. Ahmed 2004 Characterization of a BMC Medical 2.64 5: 1 - 8 Xiaoyan Cindy Li full Length TMPRSS3 Genetics Shontell D. Powell isoform and identifica- Saima Riazuddin tion of mutant alleles Terry-Lynn Young responsible for non- Khushnooda Ramzan syndromic recessive Zahoor Ahmed deafness in Newfoundland Sandra Luscombe and Pakistan Kiran Dhillon Linda MacLaren Barbara Ploplis Lawrence I Shotland Elizabeth Ives Sheikh Riazuddin Thomas B. Friedman Robert J. Morell Edward R. Wilcox

Zhang, Q., 2004 Autosomal recessive Molecular 2.239 10 884-9. Zulfiqar, F., retinitis pigmentosa Vision Riazuddin, S.A., in a Pakistani family Xiao, X., mapped to CNGA1 19 -

Ahmad, Z., with identification of Riazuddin, S., a novel mutation. Hejtmancik, J.F.

. Shahzad, M. S., 2004 Population Studies of Arch. 54(4) 215-22 S. Yassir Abbas, STR Loci Med. Abdul Qayyum Rao, D3S1358, D5S818, Sadowej M. Hashim Raza, D7S820, D18S51 Kryminol. ObaidUllah, and FGA in NWFP Zia ur Rehman, and Sindhi A. Ali Shahid, Populations of Zahoor Ahmed Pakistan for Forensic and S. Riazuddin Use.

S. Amer Riazuddi, 2005 A New Locus for Investigative3.643 46 623-6 Afshan Yasmeen, Autosomal Recessive Ophthalmol. Qingjiong Zhang, Nuclear Cataract Mapped and Visual Wenliang Yao, to Chromosome 19q13 Science M.F.Sabar, in a Pakistani Family. Zahoor Ahmed, Sheikh Riazuddin, and J. Fielding Hejtmancik,

Asghar, M.N. 2006 2-D PAGE analysis of Anal Bioanal. 2.695384 946-50 Ashfaq, M. Ahmed, Z. pesticide-induced stress Chem. Khan, I,U. proteins of E. coli.

Qingjiong Zhang 2007 Severe retinitis pigmentosa Human 4.331 Fareeha Zulfiqar mapped to 4p15 Genetics Xueshan Xiao and associated with a DOI 10. S. Amer Riazuddin novel mutation in the 1007/s00439 Zahoor Ahmad PROM1 gene. -007-0395-2 Raphael Caruso Ian MacDonald Paul Sieving 20 -

Sheikh Riazuddin J. Fielding Hejtmancik

Faraz Malik, 2008 Developmentof 19-plex . ACAD J 20 267-73 Mahmood A. Kayani Y STR system and . XJTU , M. Ansar, Obaid Ullah polymorphism studies , Muhammad Shafeeq in Pakistani population. Shahid Chohan, , Yasir Abbas, , Saqib Shahzad , Ali Raza, Rahat Rehman , Faizan Raiz, Muhammad Hassan siddiqi , Allah Rakha , Zia ur Rehman and Zahoor Ahmed TOTAL impact factor >63.280