Targeted Exome Capture and Sequencing Identifies Novel
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Organ Level Protein Networks As a Reference for the Host Effects of the Microbiome
Downloaded from genome.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press 1 Organ level protein networks as a reference for the host effects of the microbiome 2 3 Robert H. Millsa,b,c,d, Jacob M. Wozniaka,b, Alison Vrbanacc, Anaamika Campeaua,b, Benoit 4 Chassainge,f,g,h, Andrew Gewirtze, Rob Knightc,d, and David J. Gonzaleza,b,d,# 5 6 a Department of Pharmacology, University of California, San Diego, California, USA 7 b Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, 8 California, USA 9 c Department of Pediatrics, and Department of Computer Science and Engineering, University of 10 California, San Diego California, USA 11 d Center for Microbiome Innovation, University of California, San Diego, California, USA 12 e Center for Inflammation, Immunity and Infection, Institute for Biomedical Sciences, Georgia State 13 University, Atlanta, GA, USA 14 f Neuroscience Institute, Georgia State University, Atlanta, GA, USA 15 g INSERM, U1016, Paris, France. 16 h Université de Paris, Paris, France. 17 18 Key words: Microbiota, Tandem Mass Tags, Organ Proteomics, Gnotobiotic Mice, Germ-free Mice, 19 Protein Networks, Proteomics 20 21 # Address Correspondence to: 22 David J. Gonzalez, PhD 23 Department of Pharmacology and Pharmacy 24 University of California, San Diego 25 La Jolla, CA 92093 26 E-mail: [email protected] 27 Phone: 858-822-1218 28 1 Downloaded from genome.cshlp.org on October 6, 2021 - Published by Cold Spring Harbor Laboratory Press 29 Abstract 30 Connections between the microbiome and health are rapidly emerging in a wide range of 31 diseases. -
A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated. -
The Alter Retina: Alternative Splicing of Retinal Genes in Health and Disease
International Journal of Molecular Sciences Review The Alter Retina: Alternative Splicing of Retinal Genes in Health and Disease Izarbe Aísa-Marín 1,2 , Rocío García-Arroyo 1,3 , Serena Mirra 1,2 and Gemma Marfany 1,2,3,* 1 Departament of Genetics, Microbiology and Statistics, Avda. Diagonal 643, Universitat de Barcelona, 08028 Barcelona, Spain; [email protected] (I.A.-M.); [email protected] (R.G.-A.); [email protected] (S.M.) 2 Centro de Investigación Biomédica en Red Enfermedades Raras (CIBERER), Instituto de Salud Carlos III (ISCIII), Universitat de Barcelona, 08028 Barcelona, Spain 3 Institute of Biomedicine (IBUB, IBUB-IRSJD), Universitat de Barcelona, 08028 Barcelona, Spain * Correspondence: [email protected] Abstract: Alternative splicing of mRNA is an essential mechanism to regulate and increase the diversity of the transcriptome and proteome. Alternative splicing frequently occurs in a tissue- or time-specific manner, contributing to differential gene expression between cell types during development. Neural tissues present extremely complex splicing programs and display the highest number of alternative splicing events. As an extension of the central nervous system, the retina constitutes an excellent system to illustrate the high diversity of neural transcripts. The retina expresses retinal specific splicing factors and produces a large number of alternative transcripts, including exclusive tissue-specific exons, which require an exquisite regulation. In fact, a current challenge in the genetic diagnosis of inherited retinal diseases stems from the lack of information regarding alternative splicing of retinal genes, as a considerable percentage of mutations alter splicing Citation: Aísa-Marín, I.; or the relative production of alternative transcripts. Modulation of alternative splicing in the retina García-Arroyo, R.; Mirra, S.; Marfany, is also instrumental in the design of novel therapeutic approaches for retinal dystrophies, since it G. -
Mrna Editing, Processing and Quality Control in Caenorhabditis Elegans
| WORMBOOK mRNA Editing, Processing and Quality Control in Caenorhabditis elegans Joshua A. Arribere,*,1 Hidehito Kuroyanagi,†,1 and Heather A. Hundley‡,1 *Department of MCD Biology, UC Santa Cruz, California 95064, †Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University, Tokyo 113-8510, Japan, and ‡Medical Sciences Program, Indiana University School of Medicine-Bloomington, Indiana 47405 ABSTRACT While DNA serves as the blueprint of life, the distinct functions of each cell are determined by the dynamic expression of genes from the static genome. The amount and specific sequences of RNAs expressed in a given cell involves a number of regulated processes including RNA synthesis (transcription), processing, splicing, modification, polyadenylation, stability, translation, and degradation. As errors during mRNA production can create gene products that are deleterious to the organism, quality control mechanisms exist to survey and remove errors in mRNA expression and processing. Here, we will provide an overview of mRNA processing and quality control mechanisms that occur in Caenorhabditis elegans, with a focus on those that occur on protein-coding genes after transcription initiation. In addition, we will describe the genetic and technical approaches that have allowed studies in C. elegans to reveal important mechanistic insight into these processes. KEYWORDS Caenorhabditis elegans; splicing; RNA editing; RNA modification; polyadenylation; quality control; WormBook TABLE OF CONTENTS Abstract 531 RNA Editing and Modification 533 Adenosine-to-inosine RNA editing 533 The C. elegans A-to-I editing machinery 534 RNA editing in space and time 535 ADARs regulate the levels and fates of endogenous dsRNA 537 Are other modifications present in C. -
Disrupted Alternative Splicing for Genes Implicated in Splicing and Ciliogenesis Causes PRPF31 Retinitis Pigmentosa
ARTICLE DOI: 10.1038/s41467-018-06448-y OPEN Disrupted alternative splicing for genes implicated in splicing and ciliogenesis causes PRPF31 retinitis pigmentosa Adriana Buskin et al.# Mutations in pre-mRNA processing factors (PRPFs) cause autosomal-dominant retinitis pigmentosa (RP), but it is unclear why mutations in ubiquitously expressed genes cause non- 1234567890():,; syndromic retinal disease. Here, we generate transcriptome profiles from RP11 (PRPF31- mutated) patient-derived retinal organoids and retinal pigment epithelium (RPE), as well as Prpf31+/− mouse tissues, which revealed that disrupted alternative splicing occurred for specific splicing programmes. Mis-splicing of genes encoding pre-mRNA splicing proteins was limited to patient-specific retinal cells and Prpf31+/− mouse retinae and RPE. Mis-splicing of genes implicated in ciliogenesis and cellular adhesion was associated with severe RPE defects that include disrupted apical – basal polarity, reduced trans-epithelial resistance and phagocytic capacity, and decreased cilia length and incidence. Disrupted cilia morphology also occurred in patient-derived photoreceptors, associated with progressive degeneration and cellular stress. In situ gene editing of a pathogenic mutation rescued protein expression and key cellular phenotypes in RPE and photoreceptors, providing proof of concept for future therapeutic strategies. Correspondence and requests for materials should be addressed to S.-N.G. (email: [email protected]) or to C.A.J. (email: [email protected]) or to M.L. (email: [email protected]). #A full list of authors and their affliations appears at the end of the paper. NATURE COMMUNICATIONS | (2018) 9:4234 | DOI: 10.1038/s41467-018-06448-y | www.nature.com/naturecommunications 1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/s41467-018-06448-y etinitis pigmentosa (RP) is one of the most common c.522_527+10del heterozygous mutation (Supplementary Rinherited forms of retinal blindness with a prevalence of Data 1). -
RBMX Family Proteins Connect the Fields of Nuclear RNA Processing
International Journal of Biochemistry and Cell Biology 108 (2019) 1–6 Contents lists available at ScienceDirect International Journal of Biochemistry and Cell Biology journal homepage: www.elsevier.com/locate/biocel RBMX family proteins connect the fields of nuclear RNA processing, disease and sex chromosome biology T ⁎ David J. Elliott , Caroline Dalgliesh, Gerald Hysenaj, Ingrid Ehrmann Institute of Genetic Medicine, Newcastle University, Newcastle-upon-Tyne, NE1 3BZ, UK ARTICLE INFO ABSTRACT Keywords: RBMX is a ubiquitously expressed nuclear RNA binding protein that is encoded by a gene on the X chromosome. RNA splicing RBMX belongs to a small protein family with additional members encoded by paralogs on the mammalian Y Gene expression chromosome and other chromosomes. These RNA binding proteins are important for normal development, and Brain development also implicated in cancer and viral infection. At the molecular level RBMX family proteins contribute to splicing Testis control, transcription and genome integrity. Establishing what endogenous genes and pathways are controlled by Cancer RBMX and its paralogs will have important implications for understanding chromosome biology, DNA repair and mammalian development. Here we review what is known about this family of RNA binding proteins, and identify important current questions about their functions. 1. Introduction et al., 1993). While RBMX is expressed ubiquitously through the body, RBMY genes are specifically expressed in germ cells (cells that even- RBMX (an acronym of RNA Binding Motif protein, X-linked) was tually develop into sperm). Multiple RBMY genes are present on the originally identified as one of a functionally diverse group of nuclear long arm of the human Y chromosome, each encoding 496 amino acid proteins that bind to polyadenylated RNA. -
Premature Termination Codons in PRPF31 Cause Retinitis Pigmentosa Via Haploinsufficiency Due to Nonsense-Mediated Mrna Decay Thomas Rio Frio,1 Nicholas M
Research article Premature termination codons in PRPF31 cause retinitis pigmentosa via haploinsufficiency due to nonsense-mediated mRNA decay Thomas Rio Frio,1 Nicholas M. Wade,1 Adriana Ransijn,1 Eliot L. Berson,2 Jacques S. Beckmann,1,3 and Carlo Rivolta1 1Department of Medical Genetics, University of Lausanne, Lausanne, Switzerland. 2Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Boston, Massachusetts, USA. 3Service of Medical Genetics, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland. Dominant mutations in the gene encoding the mRNA splicing factor PRPF31 cause retinitis pigmentosa, a hereditary form of retinal degeneration. Most of these mutations are characterized by DNA changes that lead to premature termination codons. We investigated 6 different PRPF31 mutations, represented by single-base substitutions or microdeletions, in cell lines derived from 9 patients with dominant retinitis pigmentosa. Five of these mutations lead to premature termination codons, and 1 leads to the skipping of exon 2. Allele-specific measurement of PRPF31 transcripts revealed a strong reduction in the expression of mutant alleles. As a conse- quence, total PRPF31 protein abundance was decreased, and no truncated proteins were detected. Subnuclear localization of the full-length PRPF31 that was present remained unaffected. Blocking nonsense-mediated mRNA decay significantly restored the amount of mutant PRPF31 mRNA but did not restore the synthesis of mutant proteins, even in conjunction with inhibitors of protein degradation pathways. Our results indicate that most PRPF31 mutations ultimately result in null alleles through the activation of surveillance mechanisms that inactivate mutant mRNA and, possibly, proteins. Furthermore, these data provide compelling evidence that the pathogenic effect of PRPF31 mutations is likely due to haploinsufficiency rather than to gain of function. -
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US 20190255192A1 ( 19) United States (12 ) Patent Application Publication (10 ) Pub. No. : US 2019 /0255192 A1 KIRN et al. (43 ) Pub. Date : Aug . 22, 2019 ( 54 ) ADENO - ASSOCIATED VIRUS VARIANT CO7K 14 /005 ( 2006 .01 ) CAPSIDS AND METHODS OF USE THEREOF A61P 27/ 02 (2006 .01 ) (71 ) Applicant : 4D MOLECULAR THERAPEUTICS A61K 9 / 00 (2006 . 01) INC . , Emeryville, CA (US ) 2 ) U . S . CI. CPC . .. .. A61K 48 / 0016 ( 2013 .01 ) ; C12N 15 /86 (72 ) Inventors : David H . KIRN , Emeryville , CA (US ) ; (2013 .01 ) ; CO7K 14 / 005 ( 2013 .01 ) ; A61K Melissa KOTTERMAN , Emeryville , 48 / 0025 ( 2013 .01 ) ; A61K 9 /0048 (2013 . 01 ) ; CA (US ) ; David SCHAFFER , A61K 48 / 0075 ( 2013. 01 ) ; A61P 27 /02 Emeryville , CA (US ) ( 2018. 01 ) ; A61K 9 / 0019 ( 2013 .01 ) ; A61K (73 ) Assignee : 4D MOLECULAR THERAPEUTICS 48 / 0058 ( 2013 .01 ) INC . , Emeryville , CA ( US ) (21 ) Appl . No. : 16 /300 ,446 ( 57 ) ABSTRACT ( 22 ) PCT Filed : May 12 , 2017 Provided herein are variant adeno - associated virus (AAV ) ( 86 ) PCT No. : PCT/ US17 / 32542 capsid proteins having one or more modifications in amino $ 371 ( c )( 1 ) , acid sequence relative to a parental AAV capsid protein , ( 2 ) Date : Nov. 9 , 2018 which , when present in an AAV virion , confer increased infectivity of one or more types of retinal cells as compared Related U . S . Application Data to the infectivity of the retinal cells by an AA V virion (60 ) Provisional application No .62 /336 ,441 , filed on May comprising the unmodified parental capsid protein . Also 13 , 2016 , provisional application No . 62 /378 , 106 , provided are recombinant AAV virions and pharmaceutical filed on Aug. -
Minor Spliceosome Components Are Predominantly Localized in the Nucleus
Minor spliceosome components are predominantly localized in the nucleus Heli K. J. Pessa*, Cindy L. Will†, Xiaojuan Meng*‡, Claudia Schneider†§, Nicholas J. Watkins†¶, Nina Pera¨ la¨ ʈ, Mariann Nymarkʈ, Janne J. Turunen*, Reinhard Lu¨ hrmann†, and Mikko J. Frilander*,** *Institute of Biotechnology, PL 56 (Viikinkaari 9), and ʈInstitute of Biomedicine, Biomedicum Helsinki, PL 63 (Haartmaninkatu 8), University of Helsinki, FIN-00014, Helsinki, Finland; and †Max Planck Institute of Biophysical Chemistry, Department of Cellular Biochemistry, Am Fassberg 11, 37077 Go¨ttingen, Germany Communicated by James E. Dahlberg, University of Wisconsin Medical School, Madison, WI, April 15, 2008 (received for review February 22, 2008) Recently, it has been reported that there is a differential subcellular monomethyl cap and Lsm proteins in U6atac suggest that these distribution of components of the minor U12-dependent and major minor snRNPs share the same biogenesis pathways and local- U2-dependent spliceosome, and further that the minor spliceo- ization with their major counterparts. Indeed, previous studies some functions in the cytoplasm. To study the subcellular local- have localized U11 and U12 snRNAs in the nucleus in human ization of the snRNA components of both the major and minor cells (17) and U11 in the nucleus of Drosophila cells (18). spliceosomes, we performed in situ hybridizations with mouse The vast majority of snRNP and spliceosomal proteins appear tissues and human cells. In both cases, all spliceosomal snRNAs to be shared by both spliceosomes, with the notable exception of were nearly exclusively detected in the nucleus, and the minor U11 seven proteins that are specifically associated with the U11 and U12 snRNAs were further shown to colocalize with U4 and U2, and/or U11/U12 snRNPs (14, 19). -
AAV-Mediated Gene Augmentation Therapy Restores Critical Functions in Mutant Ipsc
bioRxiv preprint doi: https://doi.org/10.1101/729160; this version posted August 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Title: AAV-mediated gene augmentation therapy restores critical functions in mutant iPSC- derived PRPF31+/- cells. Short title: Gene therapy for PRPF31-linked retinopathy. Authors: Elizabeth M. Brydon1*, Revital Bronstein1*, Adriana Buskin2, Majlinda Lako2, Eric A. Pierce1, and Rosario Fernandez-Godino1. * These authors contributed equally to this work. Affiliations 1. Ocular Genomics Institute at Mass Eye and Ear-Harvard Medical School. Department of Ophthalmology. 2. Institute of Genetic Medicine, Newcastle University, UK Correspondence should be addressed to Rosario Fernandez-Godino: [email protected] 243 Charles St. Boston, MA 02114 Tel. +1 617-573-6787 Fax. +1 617-573-6901 1 bioRxiv preprint doi: https://doi.org/10.1101/729160; this version posted August 8, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. ABSTRACT Retinitis pigmentosa (RP) is the most common form of inherited vision loss and is characterized by degeneration of retinal photoreceptor cells and the retinal pigment epithelium (RPE). Mutations in pre- mRNA processing factor 31 (PRPF31) cause dominant RP via haploinsufficiency with incomplete penetrance. There is good evidence that the diverse severity of this disease is a result of differing levels of expression of the wild type allele among patients. Thus, we hypothesize that PRPF31-related RP will be amenable to treatment by adeno-associated virus (AAV)-mediated gene augmentation therapy. -
New Roles for the RNA Processing Factors Cfim68 and SRPK2 Highlight Unexpected Links in the Control of Mammalian Gene Expression
PhD Program in Translational and Molecular Medicine DIMET XXII Cycle, Academic Year 2008-2009 University of Milano-Bicocca School of Medicine and Faculty of Science New roles for the RNA processing factors CFIm68 and SRPK2 highlight unexpected links in the control of mammalian gene expression Silvia Vivarelli – No. 708258 1 2 A mio padre, Agostino 3 4 TABLE OF CONTENTS Chapter 1............................................................................................. 8 GENERAL INTRODUCTION ......................................................... 8 1. The Alternative Splicing .............................................................. 8 1.1 The Alternative Splicing Mechanism..................................... 8 1.2 The Splicing Reaction ............................................................ 9 1.3 Alternative Splicing Regulation ........................................... 13 1.4 The SR Family of Proteins ................................................... 21 1.4.1 SR Proteins: an overview .................................................. 21 1.4.2 SR Proteins: a Vertical Integration of Gene Expression ... 25 1.4.3 SR Protein Kinases............................................................ 26 1.5 Signal Transduction Pathways: from Extracellular Stimuli to Alternative Splicing.................................................................... 29 1.6 Stressful Splicing: the Effects of Paraquat ........................... 30 2. The 3’ End Formation and Export.............................................. 31 2.1 Molecular -
A Novel Mutation in PRPF31, Causative of Autosomal Dominant Retinitis Pigmentosa, Using the BGISEQ-500 Sequencer
Int J Ophthalmol, Vol. 11, No. 1, Jan.18, 2018 www.ijo.cn Tel:8629-82245172 8629-82210956 Email:[email protected] ·Basic Research· A novel mutation in PRPF31, causative of autosomal dominant retinitis pigmentosa, using the BGISEQ-500 sequencer Yu Zheng1,2,3, Hai-Lin Wang4, Jian-Kang Li2,3, Li Xu4, Laurent Tellier2,3, Xiao-Lin Li4, Xiao-Yan Huang1,2,3, Wei Li2,3, Tong-Tong Niu4, Huan-Ming Yang2,5, Jian-Guo Zhang2,3, Dong-Ning Liu4 1BGI Education Center, University of Chinese Academy of probands, as well as upon other family members. This Sciences, Shenzhen 518083, China novel, pathogenic genotype co-segregated with retinitis 2 BGI-Shenzhen, Shenzhen 518083, China pigmentosa phenotype in these two families. 3 China National GeneBank, BGI-Shenzhen, Shenzhen 518120, ● CONCLUSION: ADRP is a subtype of retinitis pigmentosa, China defined by its genotype, which accounts for 20%-40% 4 The Fourth People's Hospital of Shenyang, Shenyang 110031, of the retinitis pigmentosa patients. Our study thus Liaoning Province, China expands the spectrum of PRPF31 mutations known to 5 James D. Watson Institute of Genome Sciences, Hangzhou occur in ADRP, and provides further demonstration of the 310058, China applicability of the BGISEQ500 sequencer for genomics Co-first authors: Yu Zheng and Hai-Lin Wang research. Correspondence to: Dong-Ning Liu. The Fourth People's ● KEYWORDS: retinitis pigmentosa; PRPF31; BGISEQ-500 Hospital of Shenyang, Shenyang 110031, Liaoning Province, DOI:10.18240/ijo.2018.01.06 China. [email protected]; Jian-Guo Zhang. China National GeneBank, BGI-Shenzhen, Shenzhen 518120, China. Citation: Zheng Y, Wang HL, Li JK, Xu L, Tellier L, Li XL, Huang [email protected] XY, Li W, Niu TT, Yang HM, Zhang JG, Liu DN.