Homogeneous Nuclear Background for Mitochondrial Cline in Northern Range of Notochthamalus Scabrosus

Total Page:16

File Type:pdf, Size:1020Kb

Homogeneous Nuclear Background for Mitochondrial Cline in Northern Range of Notochthamalus Scabrosus INVESTIGATION Homogeneous Nuclear Background for Mitochondrial Cline in Northern Range of Notochthamalus scabrosus Christina Zakas,* Ken Jones,† and John P. Wares‡,1 *New York University, Center for Genomics and Systems Biology, New York, New York 10003, †University of Colorado, Aurora, Colorado 80045, and ‡University of Georgia, Athens, Georgia 30602 ABSTRACT A mitochondrial cline along the Chilean coast in the barnacle Notochthamalus scabrosus KEYWORDS suggests a species history of transient allopatry and secondary contact. However, previous studies of RAD-seq nuclear sequence divergence suggested population genetic homogeneity across northern and central SNP Chile. Here, we collect single-nucleotide polymorphism data from pooled population samples sequenced cytonuclear with restriction site2associated DNA sequencing procedures, confirm these data with the use of a Golden- disequilibrium Gate array, and identify a discordance between population genetic patterns in the nuclear and mitochon- barnacle drial genomes. This discordance was noted in previous work on this species, but here it is confirmed that the Chile nuclear genome exhibits only slight phylogeographic variation across 3000 km of coastline, in the presence of a strong and statistically significant mitochondrial cline. There are nevertheless markers (approximately 5% of nuclear single-nucleotide polymorphisms) exhibiting cytonuclear disequilibrium relative to mitotype. Although these data confirm our previous explorations of this species, it is likely that some of the nuclear genomic diversity of this species has yet to be explored, as comparison with other barnacle phylogeography studies suggest that a divergence of similar magnitude should be found in the nuclear genome somewhere else in the species range. Patterns of mitochondrial diversity have heralded great discoveries in Pavlova et al. 2013). For example, divergence of mitochondrial lin- biodiversity and often are used to indicate the likelihood that evolu- eages in an Australian robin (Eopsaltria australis) exhibits an east- tionarily distinct lineages are harbored within recognized morpholog- west pattern, perpendicular to the latitudinal structure of nuclear ical species (Hebert et al. 2004; Moritz and Cicero 2004). However, markers (Pavlova et al. 2013). divergence of mitochondrial lineages is not necessarily representative of Another such case of discordance has been identified in the the rest of a eukaryotic genome (Hudson and Coyne 2002; Hickerson barnacle Notochthamalus scabrosus (Foster and Newman 1987) along et al. 2006). In some cases, exploration of divergence at nuclear the central coast of Chile (Zakas et al. 2009). Two divergent mito- markers supports a general pattern (Tellier et al. 2009), but in other chondrial lineages are found in this species, with one of them only cases the two genomic compartments appear to have wildly distinct found in natural populations south of ~30°S(Zakaset al. 2009; Laughlin histories of isolation and/or selection (Toews and Brelsford 2012; et al. 2012). A nuclear gene (elongation factor 1-a; nEF1) surveyed for the same region showed no such geographic pattern of genetic fi Copyright © 2014 Zakas et al. diversi cation (Zakas et al. 2009), despite the capacity for this gene doi: 10.1534/g3.113.008383 region to mirror such patterns and magnitudes of divergence in Manuscript received September 9, 2013; accepted for publication November 25, other coastal barnacles (Sotka et al. 2004). Because the nEF1 data 2013; published Early Online December 17, 2013. did not exhibit the expected genealogical divergence, as seen in other This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/ barnacle species with similar mitochondrial clines (Sotka et al. by/3.0/), which permits unrestricted use, distribution, and reproduction in any 2004), it is clear that a better representation of diversity and diver- medium, provided the original work is properly cited. gence in the nuclear genome will be needed to understand the Supporting information is available online at http://www.g3journal.org/lookup/ phylogeography of N. scabrosus. suppl/doi:10.1534/g3.113.008383/-/DC1. Such a result suggests a discordant history for the mitochondrial Data from this article are available NCBI BioProject under PRJNA227359. 1Corresponding author: Department of Genetics, University of Georgia, Athens, GA and nuclear genomes of N. scabrosus, but of course one nuclear locus 30602. E-mail: [email protected] is not sufficiently representative of the dynamics of the genome. Volume 4 | February 2014 | 225 Recent advances in DNA sequencing technology (Schuster 2008) for individuals comprised solely of the A1, A2 (“northern”), and B make it readily possible to efficiently survey a larger fraction of the (“southern”) mitochondrial haplotypes as described in Laughlin genome for population genetic analysis. Elucidating the pattern of et al. (2012). population divergence by the use of many thousands of nuclear loci Pooled genomic DNA were cut per standard protocols (Peterson reveals the potential combinations of ecological and evolutionary et al. 2012) by the use of SpeI and Sau3AI, chosen for their utility in forces that drive this mismatch in genome histories. Here we use similar projects (K. Jones, unpublished results). Libraries were then a combination of reduced-representation genome sequencing (restric- prepared for Illumina sequencing by the use of TruSeq adapters and tion site2associated DNA sequencing, or RADseq; Peterson et al. 100-bp paired ends were sequenced on an Illumina HiSequation 2500 2012) of population samples and direct multilocus genotyping of at the University of Colorado at Denver core facility. markers developed from such data to find nuclear regions that may be associated with the divergent mitochondrial lineages found in Genome-wide single-nucleotide polymorphism N. scabrosus. Because the aforementioned nEF1 data did not exhibit (SNP) analysis a cline, we were interested in determining the extent to which a large RADseq data were trimmed to remove adapter sequence and error- representation of the nuclear genome displayed a concordant pattern. prone regions. Sequence fragments were aligned and organized with We also assess the utility of pooled RADseq data by comparison with the program STACKS (Catchen et al. 2013). Because these data rep- direct genotyping of individuals in a subset of populations; the op- resent pooled samples from distinct locations, the consensus stacks portunity to collect appropriate data via pooled sampling, if it provides were used as the reference library in the program POPOOLATION2 (Kofler fi results consistent with individual genotypes, could be an ef cient and et al. 2011), which aligns reads from pooled libraries to these reference inexpensive way to identify genome-wide diversity in nonmodel sequences and generates allele frequency and other population genetic organisms. statistics such as FST. SNPs were only included for analysis with a minimum fragment coverage of 50 and minimum quality score of 20. METHODS Specimen collection and sequencing SNP selection Collected specimens and DNA isolation protocols are described in To genotype at the individual level, SNPs were chosen for assay using detail in Laughlin et al. (2012). Sample locations are shown in Figure 1. the Illumina BeadXPress platform as in Zakas and Wares (2012). Pooled samples of each location, with separate pools for “low” and Thus, SNPs were identified that had sequenced flanking regions of “high” intertidal samples from Temblador and Guanaqueros, were .30 bp for primer design; these were scored by Illumina for their generated for each site with equimolar concentrations of each in- probability of success. Only SNPs with the greatest possible quality dividual. All individuals included had been previously sequenced at ranking were selected for further analysis. We chose 192 for multi- mitochondrial cytochrome oxidase I (COI) and identified to mito- locus genotyping, setting specific aprioricriteria for selection follow- chondrial type. Three separate genomic DNA pools were generated ing Zakas et al. (2012), where ribosomal markers were eliminated and a balance of SNPs from coding regions and anonymous regions, as well as apparent nonsynonymous polymorphisms and synonymous polymorphisms were chosen. Consensus sequences for each region were evaluated with NCBI BLAST; any contig that blasted to a mito- chondrial region with score e , 0.001 was considered a likely mito- chondrial region and was excluded. We further screened the SNP library for potential outliers that exhibit high FST values among locations using the program BAYESSCAN (Foll and Gaggiotti 2008); these outliers were included in the BeadX- Press assay. To minimize potential ascertainment biases in selection of SNPs for completion of the array, SNPs with minor allele frequencies of 0.3020.35, 0.3620.40, 0.4120.45, and 0.4620.50 were equally represented. Markers with lower minor allele frequencies were elim- inated to guard against sequencing artifacts as in Zakas et al. (2012). Further, only one SNP was selected from each identified sequence “stack” (contig) for the BeadXPress array to minimize linkage disequi- librium among markers. BeadXPress assay data Genomic DNA samples at 20250 ng/mL for individuals from three representative locations (Arica, Las Cruces, and Niebla; Figure 1) were prepared by the Georgia Genomics Facility and run on a BeadXPress platform
Recommended publications
  • ART/SMSG/SAERI Expedition Report: Hummock Island February 2021
    ART/SMSG/SAERI Expedition Report: Hummock Island February 2021 Significance of peat dust and terrestrial erosion for marine communities around Hummock Island Amy Guest, Dr Paul Brewin, Dr Paul Brickle, Dr Karen von Juterzenka, and Dr Klemens Pütz Cosmasterias lurida (beaded starfish) and Munida gregaria (lobster krill) on a peat covered sandy substrate, Hummock Island February 2021 ART/SMSG/SAERI expedition report: Hummock Island, February 2021 Logistics Expedition dates: 4 - 14th Feb 2021 (for Daily Log see Appendix 1; Dive log see Appendix 2) Vessels: SMSG Fram (5.8 m RHIB), launched from Roy Cove; Sailing Yacht Porvenir II. Accommodation: Roy Cove self-catering, ART House Hummock Island Participants: Dr Paul Brickle (Co-PI) Dr Paul Brewin (Co-PI) Steve Cartwright (Dive Officer / Coxswain) Joost Pompert (Scientist / Surveyor) Sacha Cleminson (Scientist / Surveyor) 4th – 8th February, N.B. flew out from Fox Bay. Amy Guest (PhD Student / Surveyor / Logistics) Sally Poncet (Antarctic Research Trust) Ken Passfield (Antarctic Research Trust) Background Hummock Island lies to the west of West Falkland (Figure 1). Like on other islands in the Falklands, Hummock Island´s rocky surface is covered by peat soil. Decades of grazing on the island has led to de- vegetation of about one third of the 303 ha and subsequent substantial erosion. Large areas were replaced by black ground indicating the extension and distribution of exposed peat soil. The Antarctic Research Trust (ART) is currently re-vegetating the island by tussac planting campaigns. Tussac roots and above ground blade structures will stabilise the peat soil and, moreover, will prove very efficient in storage of atmospheric carbon.
    [Show full text]
  • Benthic Assemblages in South American Intertidal Rocky Shores: Biodiversity, Services, and Threats
    See discussions, stats, and author profiles for this publication at: https://www.researchgate.net/publication/299507578 Benthic Assemblages in South American Intertidal Rocky Shores: Biodiversity, Services, and Threats Chapter · January 2016 READS 318 29 authors, including: Gregorio Bigatti Alvar Carranza IBIOMAR- Instituto de Biología de Organismo… University of the Republic, Uruguay 82 PUBLICATIONS 563 CITATIONS 96 PUBLICATIONS 814 CITATIONS SEE PROFILE SEE PROFILE Tito Lotufo Erasmo C Macaya University of São Paulo University of Concepción 37 PUBLICATIONS 216 CITATIONS 63 PUBLICATIONS 441 CITATIONS SEE PROFILE SEE PROFILE All in-text references underlined in blue are linked to publications on ResearchGate, Available from: Alvar Carranza letting you access and read them immediately. Retrieved on: 26 May 2016 Chapter BENTHIC ASSEMBLAGES IN SOUTH AMERICAN INTERTIDAL ROCKY SHORES: BIODIVERSITY, SERVICES, AND THREATS Patricia Miloslavich1,2, Juan José Cruz-Motta,1,3, Alejandra Hernández1,4, César Herrera1, Eduardo Klein1, Francisco Barros5, Gregorio Bigatti6, Maritza Cárdenas7, Alvar Carranza8, Augusto Flores9, Patricia Gil10, Judith Gobin11, Jorge Gutiérrez12, Marcos Krull5, Juan F. Lazarus13, Edgardo Londoño13, Tito Lotufo9, Erasmo Macaya14, Elba Mora15, Sergio Navarrete16, Gabriela Palomo17, Mirtala Parragué16, Franciane Pellizzari18, Rosana Rocha19, Leonardo Romero20, Roberto Retamales21, Roger Sepúlveda22, Michelle C. Silva18 and Sabrina Soria17 1Universidad Simón Bolívar, Caracas, Venezuela 2Australian Institute of Marine Science,
    [Show full text]
  • Homogeneous Nuclear Background for Mitochondrial Cline in Northern Range of Notochthamalus Scabrosus
    G3: Genes|Genomes|Genetics Early Online, published on December 17, 2013 as doi:10.1534/g3.113.008383 HOMOGENEOUS NUCLEAR BACKGROUND FOR MITOCHONDRIAL CLINE IN NORTHERN RANGE OF NOTOCHTHAMALUS SCABROSUS Manuscript for G3 Version 11/26/13 Zakas, C.1 Jones, K.2 Wares, J. P.3 1 New York University, Center for Genomics and Systems Biology, NY, NY 10003 2 University of Colorado, Aurora, CO 80045 3 University of Georgia, Athens, GA 30602 Data available in public repositories: NCBI BioProject PRJNA227359 © The Author(s) 2013. Published by the Genetics Society of America. Running Title: Low SNP variation in Notochthamalus Keywords: RAD-seq, SNP, cytonuclear disequilibrium, barnacle, Chile Corresponding author: J. P. Wares Department of Genetics University of Georgia Athens, Georgia, USA 30602 [email protected] 2 Abstract A mitochondrial cline along the Chilean coast in the barnacle Notochthamalus scabrosus suggests a species history of transient allopatry and secondary contact. However, previous studies of nuclear sequence divergence suggested population genetic homogeneity across northern and central Chile. Here we collect SNP data from pooled population samples sequenced with RAD-seq procedures, confirm these data using a GoldenGate array, and identify a discordance between population genetic patterns in the nuclear and mitochondrial genomes. This discordance was noted in previous work on this species, but here it is confirmed that the nuclear genome exhibits only slight phylogeographic variation across 3000km of coastline, in the presence of a strong and statistically significant mitochondrial cline. There are nevertheless markers (approximately 5% of nuclear SNPs) exhibiting cytonuclear disequilibrium relative to mitotype. Although these data confirm our previous explorations of this species, it is likely that some of the nuclear genomic diversity of this species has yet to be explored, as comparison with other barnacle phylogeography studies suggest that a divergence of similar magnitude should be found in the nuclear genome somewhere else in the species range.
    [Show full text]
  • The Sessile Organisms Society of Japan
    Sessile Organisms 23(1):1-15 (2006) The Sessile Organisms Society of Japan フジツボ類の分類お よび鑑定の手引き 山口寿之1), 久恒義之2) 1)千葉大学海洋バイ オシステム研 究セ ンター 〒263-8522 千葉 県千葉 市稲 毛区弥生町1-33 2)東京都立 向島工業 高等学校 〒131-0043東 京都墨 田区立花 四丁 目29-7 Taxonomy and identification of Japanese barnacles Toshiyuki Yamaguchi1) and Yoshiyuki Hisatsune2) 1) Marine Biosystems Research Center, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522, Japan 2) Mukohjima Technological Highschool, 4-29-7 Tachibana, Sumida, Tokyo 131-0043, Japan (Accepted December 14, 2005) (キ ー ワ ー ド: フ ジ ツ ボ 類、 分 類、 鑑 定) 蔓脚類 は固着性で、 一般 に小型の石灰質 の殻 を持つ 甲 にも生息 す る。 海洋 の プ レー ト境界、 海 洋 プ レー トの 殻類 である。 フジツボ、 カ メノテ、 エ ボシガイ がその代 (収束域 であ る) サブダ クシ ョン、 それ に伴 って形成 さ 表 で、 つるあ し類 とも呼ばれ る。固着生活へ の適応 のた れ る背弧海盆 には、海底火 山活動 または冷湧水活動 があ め、形 態の特殊化 が著 しく、 フジ ツボ類 は長い間 甲殻類 る。深海 の熱水 ・冷湧水活動域 にはそ こに固有 の原始 的 の一員 である とは思われなか った。 な蔓脚類 が生息す るこ とが1970年 代後 半知 られる よ うに 蔓脚類 は19世 紀前半まで、 軟体動物の一員 と見 な され な った。 地表近 くに棲む生物 は, みな太 陽の恵 みを受 け ていたが、 甲殻類 に共通す るノープ リウス幼生期 を経 る てい る。我 々です ら直接 または間接 に太 陽の光で光合成 ことが明 らかにな り甲殻類に含 められた。 脱皮 ご とに変 をす る植物 を摂取 して, そのエネルギーを利用 している。 態す る 自由遊泳性 の6期 の ノープ リウス幼生期 とそれ に 深海の熱水噴 出孔 には太 陽光 は到達せず, エネル ギー源 続 く1期 のキプ リス幼生期 を経て固着生活 に移 る。体 は と しての光 を利用 できない。熱水や冷湧水 に含 まれ てい 基本 的には頭 部5体 節、 胸部6体 節の体制か らな り、腹 る硫黄 な どの無機物お よび それ を摂 取す る細 菌 (バクテ 部 の体節 は退化 してい る。頭部の各体節は前か ら順 に第 リア) が, それぞれ地表 での光エネル ギーおよび植物 に 一触角、 第 二触角、 大顎、 第一小顎、 第二小顎の対 をな 相当 し, それ らが熱水噴 出孔周辺の生態系の根幹 をなす。 す付属肢 を持 つ。 大顎の前に 口が あ り、大顎 を含 めた後 そん な特異 な生態系 に全 く未知 の動物 が生息 しているこ ろ3対 の付属肢 が 口器 と呼ばれ る。胸部の各体節 には対 とだ けで なく, それ らが非常 に原始 的であるこ とに驚 き をなす二叉型 で、つ る (蔓) 状の摂食用の付属肢 を持つ。
    [Show full text]
  • John P. Wares Publications
    John P. Wares wareslab.genetics.uga.edu University of Georgia – Department of Genetics & Odum School of Ecology Education B.S. in Zoology with Honors University of Oklahoma, 1995 Ph.D. in Zoology and Genetics Duke University, 2000 Postdoctoral Training University of New Mexico PI: Tom Turner, 2000-2002 University of California, Davis PI: Rick Grosberg, 2002-2004 Academic Positions Assistant Professor University of Georgia, 2005-2011 Associate Professor University of Georgia, 2011-present Visiting Lecturer UW - Friday Harbor Labs, summer 2016 Publications (n=90; 76 while at UGA) n.b. I tend to promote students and junior colleagues via 1st authorship, with senior authorship as last. I also have a philosophical goal in publishing as much in Open Access and low-cost journals as possible. ξ equal authorship *student 2019 (0) 2018 (2) Wares, J. P., S. Crickenberger, A. F. Govindarajan, J. L. Hamrick, K. M. Skoczen, D. W. Trapnell, and D. S. Wethey. 2018. The cryptic population biology of Chthamalus fragilis Darwin, 1854 on the Atlantic coast of North America. J. Crustacean Biol., DOI: doi.org/10.1093/jcbiol/ruy070. Ewers-Saucedo, C.* and J. P. Wares. 2018. Population connectivity and phylogeography of Crustaceans. In: G. Poore and M. Thiel, eds. Evolution and Biogeography. The Natural History of the Crustacea, vol. 8. in press. 2017 (5) Ewers-Saucedo, C*., B. K. K. Chan, J. D. Zardus, and J. P. Wares. 2017. Parallel patterns of host-specific morphology and genetic admixture in sister lineages of a commensal barnacle. Biol. Bull. 232:171-185. Chandler, V. K*. and J. P. Wares.
    [Show full text]
  • The Oceanic Concordance of Phylogeography and Biogeography: a Case Study in Notochthamalus Christine Ewers-Saucedo1, James M
    The oceanic concordance of phylogeography and biogeography: a case study in Notochthamalus Christine Ewers-Saucedo1, James M. Pringle2, Hector H. Sepulveda 3, James E. Byers4, Sergio A. Navarrete5 & John P. Wares4,6 1College of Biological Sciences, University of California, Davis, California 95616 2Institute for the Study of Earth, Ocean, and Space, University of New Hampshire, Durham, New Hampshire 03824 3Departamento de Geofisica, Universidad de Concepcion, Concepcion, Chile 4Odum School of Ecology, University of Georgia, Athens, Georgia 30602 5Estacion Costera de Investigaciones Marinas Las Cruces and Center for Marine Conservation, Pontificia Universidad Catolica de Chile, Casilla 114D, Santiago, Chile 6Department of Genetics, University of Georgia, Athens, Georgia 30602 Keywords Abstract Biogeography, Chile, connectivity, Pacific Ocean, population genetics. Dispersal and adaptation are the two primary mechanisms that set the range dis- tributions for a population or species. As such, understanding how these mecha- Correspondence nisms interact in marine organisms in particular – with capacity for long-range John P. Wares, Department of Genetics, dispersal and a poor understanding of what selective environments species are University of Georgia, Athens, GA 30602. responding to – can provide useful insights for the exploration of biogeographic Tel: 706-542-7720; patterns. Previously, the barnacle Notochthamalus scabrosus has revealed two Fax: 706-542-8000; evolutionarily distinct lineages with a joint distribution that suggests an associa-
    [Show full text]
  • Jehlius Cirratus[I]
    A peer-reviewed version of this preprint was published in PeerJ on 7 February 2017. View the peer-reviewed version (peerj.com/articles/2971), which is the preferred citable publication unless you specifically need to cite this preprint. Guo B, Wares JP. 2017. Large-scale gene flow in the barnacle Jehlius cirratus and contrasts with other broadly-distributed taxa along the Chilean coast. PeerJ 5:e2971 https://doi.org/10.7717/peerj.2971 1 RH: Phylogeography Jehlius in cirratus 2 Large-­‐scale gene flow in the barnacle Jehlius cirratus and contrasts 3 with other broadly-­‐distributed taxa along the Chilean coast 4 5 Guo B.1,2 6 Wares J. P.* 2 7 8 1 National Engineering Research Center of Maricultural Facilities of China, College of 9 Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316004, PR 10 China 11 2 Department oF Genetics, University oF Georgia, Athens, Georgia, USA 12 13 *corresponding author: John P. Wares, University [email protected] of Georgia, 14 15 Key Words: phylogeography, gene FloW, barnacle, chthamalid, Chile, biogeography 16 1 PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.596v4 | CC BY 4.0 Open Access | rec: 12 Sep 2016, publ: 12 Sep 2016 17 Abstract 18 We evaluate the population genetic structure of the intertidal Jehlius barnacle 19 cirratus across a broad portion oF its geographic on distributi using data From the 20 mitochondrial cytochrome oxidase I (COI) gene region. Despite sampling diversity 21 From over 3000km oF the linear range oF this species, there is only slight regional 22 structure indicated, With Φ overall CT of 0.036 (p<0.001) yet no support for isolation 23 by distance.
    [Show full text]
  • Zoogeografía De Macroinvertebrados Bentónicos De La Costa De Chile: Contribución Para La Conservación Marina
    Revista Chilena de Historia Natural 73: 99-129, 2000 Zoogeografía de macroinvertebrados bentónicos de la costa de Chile: contribución para la conservación marina Zoogeography of benthic macroinvertebrates of the Chilean coast: contribution for marine conservation DOMINGO A. LANCELLOTII & JULIO A. VASQUEZ 1 Departamento de Biología Marina, Facultad de Ciencias del Mar, Universidad Católica del Norte, Casilla 117, Coquimbo, Chile, e-mail: [email protected] RESUMEN La diversidad de macroinvertebrados marinos ha recibido una atención creciente, no obstante, con un escaso tratamiento en el contexto biogeográfico. Este estudio analiza los registros de 1.601 especies de macroinvertebrados bentónicos pertenecientes a: Demospongiae, Anthozoa, Polychaeta, Mollusca, Crustacea, Echinodermata y Ascideacea, agrupados en 10 zonas y tratados desde una perspectiva zoogeográfica. Mollusca (611 especies), Polychaeta (403) y Crustacea (370) corresponden a los grupos mejor representados a lo largo de la costa chilena, determinantes en el patrón global de la biodiversidad. Este aumenta suavemente de norte a sur, interrumpido por máximos que sugieren esfuerzos diferenciales de estudio más que un comportamiento natural de la biodiversidad. El grado de agrupamiento entre las zonas muestra las tres unidades biogeográficas definidas recientemente por Lancellotti & V ásquez. Este arreglo, que representa lo exhibido por los grupos más diversos, se ve alterado en los grupos menos representados donde las diferencias obedecen al patrón de afinidades mostradas por las zonas comprendidas dentro de la Región Templada Transicional. El quiebre zoogeográfico alrededor de los 41° S, sugerido largamente en la literatura, sólo ocurre en Echinodermata y Demospongiae, evidenciando en los otros taxa la existencia de un área de transición entre los 35° y 48° S, caracterizada por un reemplazo gradual de especies.
    [Show full text]
  • Trophic Interactions and Food Web Structure of a Subantarctic Marine Food Web in the Beagle Channel: Bahı´A Lapataia, Argentina
    Polar Biol DOI 10.1007/s00300-016-2007-x ORIGINAL PAPER Trophic interactions and food web structure of a subantarctic marine food web in the Beagle Channel: Bahı´a Lapataia, Argentina 1,2 3 4 1,2 Luciana Riccialdelli • Seth D. Newsome • Marilyn L. Fogel • Daniel A. Ferna´ndez Received: 26 May 2015 / Revised: 7 July 2016 / Accepted: 8 July 2016 Ó Springer-Verlag Berlin Heidelberg 2016 Abstract Basic ecological knowledge regarding the expected interactions among species based on independent importance of different sources of primary production and information. Using Bayesian isotope mixing models, we how it is transferred among consumer species is required to estimated that the proportional contribution of benthic properly manage and conserve subpolar ecosystems that production, in particular through grazing and the con- are particularly vulnerable to environmental changes. We sumption of detritus, was a more important source of used carbon (d13C) and nitrogen (d15N) stable isotope energy for primary and secondary consumers than pelagic analysis to establish a baseline of species interactions and production. Using stable isotope analysis to continually food web structure for the nearshore marine community in monitor species interactions and food web structure may Bahia Lapataia, Argentina, an ecosystem that faces many prove to be a valuable research and management tool for threats, including a recent invasion of exotic chinook sal- assessing ecological consequences of different threats in mon. Primary producers and other organic sources (e.g., this and other subpolar ecosystems. particulate organic matter) available to the food web were isotopically distinct and had a wide range in d13C(-31.3 to Keywords Food web Á Community structure Á Trophic -5.3 %) and d15N(-0.5 to 13.1 %) values.
    [Show full text]
  • Oceanography and Marine Biology an Annual Review Volume 58
    Oceanography and Marine Biology An Annual Review Volume 58 Edited by S. J. Hawkins, A. L. Allcock, A. E. Bates, A. J. Evans, L. B. Firth, C. D. McQuaid, B. D. Russell, I. P. Smith, S. E. Swearer, P. A. Todd First edition published 2021 ISBN: 978-0-367-36794-7 (hbk) ISBN: 978-0-429-35149-5 (ebk) Chapter 1 The Biology of Austrominius Modestus (Darwin) in its Native and Invasive Range Ruth M. O’Riordan, Sarah C. Culloty, Rob Mcallen & Mary Catherine Gallagher (CC BY-NC-ND 4.0) This OA chapter is funded by University College Cork Oceanography and Marine Biology: An Annual Review, 2020, 58, 1–78 © S. J. Hawkins, A. L. Allcock, A. E. Bates, A. J. Evans, L. B. Firth, C. D. McQuaid, B. D. Russell, I. P. Smith, S. E. Swearer, P. A. Todd, Editors Taylor & Francis THE BIOLOGY OF AUSTROMINIUS MODESTUS (DARWIN) IN ITS NATIVE AND INVASIVE RANGE RUTH M. O’RIORDAN, SARAH C. CULLOTY, ROB MCALLEN & MARY CATHERINE GALLAGHER School of Biological, Earth and Environmental Sciences and the Environmental Research Institute, University College Cork, Cork, Ireland Abstract Austrominius modestus, formerly Elminius modestus, is a relatively small species of four-plated acorn barnacle, which is native to the subtropical and temperate zones of Australasia. It was introduced into Europe in the 1940s, where its current range includes England, Scotland, Wales, Ireland and continental Europe from Denmark to southern Portugal, as well as two reported locations in the Mediterranean Sea. This species occurs intertidally and subtidally on a very wide range of substrata in both its native and introduced range and is found on sheltered to intermediate exposed shores, but is absent from wave-exposed shores, probably due to the relative fragility of its shell.
    [Show full text]
  • Marjorie F. Oleksiak Om P. Rajora Editors Population Genomics: Marine Organisms
    Population Genomics Editor-in-Chief: Om P. Rajora Marjorie F. Oleksiak Om P. Rajora Editors Population Genomics: Marine Organisms Population Genomics Editor-in-Chief Om P. Rajora Faculty of Forestry and Environmental Management University of New Brunswick Fredericton, NB, Canada This pioneering Population Genomics Series deals with the concepts and approaches of population genomics and their applications in addressing fundamental and applied topics in a wide variety of organisms. Population genomics is a fast emerging discipline, which has created a paradigm shift in many fields of life and medical sciences, including population biology, ecology, evolution, conservation, agricul- ture, horticulture, forestry, fisheries, human health and medicine. Population genomics has revolutionized various disciplines of biology including population, evolutionary, ecological and conservation genetics, plant and animal breeding, human health, genetic medicine, and pharmacology by allowing to address novel and long-standing intractable questions with unprecedented power and accu- racy. It employs large-scale or genome-wide genetic information across individuals and populations and bioinformatics, and provides a comprehensive genome-wide perspective and new insights that were not possible before. Population genomics has provided novel conceptual approaches, and is tremen- dously advancing our understanding the roles of evolutionary processes, such as mutation, genetic drift, gene flow, and natural selection, in shaping up genetic variation at individual loci and across the genome and populations, disentangling the locus-specific effects from the genome-wide effects, detecting and localizing the functional genomic elements, improving the assessment of population genetic parameters or processes such as adaptive evolution, effective population size, gene flow, admixture, inbreeding and outbreeding depression, demography, and bioge- ography, and resolving evolutionary histories and phylogenetic relationships of extant and extinct species.
    [Show full text]
  • Restricted Gene Flow in Chilean Barnacles Reflects an Oceanographic and Biogeographic Transition Zone
    Vol. 394: 165–177, 2009 MARINE ECOLOGY PROGRESS SERIES Published November 18 doi: 10.3354/meps08265 Mar Ecol Prog Ser Restricted gene flow in Chilean barnacles reflects an oceanographic and biogeographic transition zone C. Zakas1, J. Binford2, S. A. Navarrete3, J. P. Wares1,* 1Department of Genetics, University of Georgia, Athens, Georgia 30602, USA 2Yale University School of Medicine, New Haven, Connecticut 06510, USA 3Estación Costera de Investigaciones Marinas & Center for Advanced Studies in Ecology and Biodiversity, Pontificia Universidad Católica de Chile, Santiago, Chile ABSTRACT: Broad scale patterns of genetic structure in coastal communities are strongly affected by both ecological transitions and larval dispersal. Along the Chilean coast, we examined 2 species of co-distributed barnacles, Jehlius cirratus and Notochthamalus scabrosus, which span an ecological transition associated with a sharp increase in larval recruitment. A distinct break in haplotype fre- quencies in the mitochondrial cytochrome oxidase I (mtCOI) gene was detected in N. scabrosus, with only marginal genetic structure observed in J. cirratus, suggesting significant differences in either selective pressures or dispersal ability between the species. The nuclear gene elongation factor 1α was also analyzed in N. scabrosus, and similarly suggested limited gene flow. The sharp genetic tran- sition is downstream of the described ecological transition, effectively mirroring a genetic cline described in a different barnacle species along the North American Pacific coast. KEY WORDS: Phylogeography · Oceanography · Intertidal · Chile · Barnacle · Upwelling · Recruitment Resale or republication not permitted without written consent of the publisher INTRODUCTION havior and ecology, as well as the effects of paleocli- mate, may generate or maintain patterns of genetic A combination of historical processes and nearshore structure that deviate from this expectation.
    [Show full text]