Recombinant Human MED4 Protein Catalog Number: ATGP0552

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Recombinant human MED4 protein Catalog Number: ATGP0552 PRODUCT INPORMATION Expression system E.coli Domain 1-270aa UniProt No. Q9NPJ6 NCBI Accession No. AAH05189 Alternative Names Mediator complex subunit 4, ARC36, DRIP36, VDRIP, Mediator complex subunit 4 Activator-recruited cofactor 36 kDa component, HSPC126, Mediator of RNA polymerase II transcription subunit 4, TRAP/SMCC/PC2 subunit p36 subunit, Vitamin D3 receptor-interacting protein complex 36 kDa component. PRODUCT SPECIFICATION Molecular Weight 30.7 kDa (278aa) confirmed by MALDI-TOF Concentration 0.5mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 100mM NaCl Purity > 80% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Mediator complex subunit 4 (MED4) is also known as mediator of RNA polymerase II transcription subunit 4 or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36). This protein is a component of the vitamin D receptor-interacting protein (DRIP) complex which functions as a nuclear receptor coactivator. The DRIP complex is capable of activating nuclear receptors in a ligand-dependent manner. Recombinant human 1 Recombinant human MED4 protein Catalog Number: ATGP0552 MED4, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Amino acid Sequence MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDGDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF LLEPPGHNKE DEDDVEIMST DSSSSSSESD <LEHHHHHH> General References Rachez C., et al. (1999) Nature 398 (6730): 824-8. Panierakis C., et al. (2009) Int J Infect Dis.13 (6):e437-43. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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UNIVERSITY of CALIFORNIA Los Angeles
UNIVERSITY OF CALIFORNIA Los Angeles Dissecting transcriptional control by Klf4 in somatic cell reprogramming A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Biological Chemistry by Huajun Zhou 2017 ABSTRACT OF THE DISSERTATION Dissecting transcriptional control by Klf4 in somatic cell reprogramming by Huajun Zhou Doctor of Philosophy in Biological Chemistry University of California, Los Angeles, 2017 Professor Gregory S. Payne, Chair Ectopic expression of four transcription factors, Oct4, Sox2, Klf4, and c-Myc, coverts somatic cells directly into induced pluripotent stem cells (iPSCs), which are functionally equivalent to embryonic stem cells (ESCs). The discovery of iPSC has been reshaping the methodology of disease modeling and drug screening in the past decade, and provides tremendous promise for regenerative medicine. However, the mechanism underlying this conversion process, reprogramming, is not yet fully understood. I aimed to dissect the reprogramming process, by characterizing the functional domains of one reprogramming factor Klf4. The transcriptional activation domain (TAD) of Klf4 was revealed to be critical for reprogramming. To search for the factors that mediates the functionality of Klf4 TAD, I identified transcriptional coactivators CBP/p300 and Mediator complex as the physical interaction partners of Klf4 TAD, and further showed that this interaction is functionally required ii for Klf4 mediated transcriptional activation in reprograming. Clathrin heavy chain, initially identified as a physical interaction partner of Klf4 TAD, was shown to be not required for Klf4 transcriptional activation. Clathrin heavy chain was furthered characterized for its potential transcriptional activation activity in CHC-TFE3, a chromosomal fusion discovered in renal cell carcinoma.
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Supplementary Material
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Biochemistry. in the Article “The Med1 Subunit of the Yeast Mediator Complex Is Involved in Both Transcriptional Activation An
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