Ethier, J., Banffy, E., Vuković, J. B., Leshtakov, K., Bacvarov, K., Roffet-Salque, M., Evershed, R., & Ivanova, M

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Ethier, J., Banffy, E., Vuković, J. B., Leshtakov, K., Bacvarov, K., Roffet-Salque, M., Evershed, R., & Ivanova, M Ethier, J., Banffy, E., Vuković, J. B., Leshtakov, K., Bacvarov, K., Roffet-Salque, M., Evershed, R., & Ivanova, M. (2017). Earliest expansion of animal husbandry beyond the Mediterranean zone in the sixth millennium BC. Scientific Reports, 7, [7146]. https://doi.org/10.1038/s41598-017-07427-x Publisher's PDF, also known as Version of record License (if available): CC BY Link to published version (if available): 10.1038/s41598-017-07427-x Link to publication record in Explore Bristol Research PDF-document This is the final published version of the article (version of record). It first appeared online via Nature at https://www.nature.com/articles/s41598-017-07427-x. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ Earliest expansion of animal husbandry beyond the Mediterranean zone in the sixth millennium BC Jonathan Ethier, Eszter Bánffy, Jasna Vuković, Krassimir Leshtakov, Krum Bacvarov, Mélanie Roffet-Salque, Richard P. Evershed, Maria Ivanova* Supplementary information Description of sites and stratigraphic contexts of pottery samples Bulgaria Yabalkovo Yabalkovo is located in the valley of Maritsa, southeast Bulgaria. The investigations at the site were part of a rescue operation due to infrastructure projects. In the years 2000-2012 more than 25 000 square meters were under exploration. In contrast to the typical tell sites from Upper Thrace, Yabalkovo is described as a “flat” settlement with a vast inhabited area which, according to the scatter of archaeological materials, was estimated to cover c. 1.5 km x 2 km. The excavations revealed several phases of occupation spanning a very long period from the early 6th millennium BC to the 11th century-first quarter of the 13th century CE (the Early and Middle Neolithic, Early Chalcolithic, Early Bronze Age, Late Iron Age, Late Roman Age, and the Middle Ages). The geophysical survey and the excavations of the Early Neolithic sites revealed two separate settled areas, each surrounded by a series of concentric ditches. There is stratigraphic evidence for three building phases.These Early Neolithic settlements belong to the Karanovo I group and the 14C dates indicate a life span along the first three centuries of the 6th millennium BC (Table S3). Samples were selected from the excavation campaigns in 2010, 2011 and 2012 and originated from two different areas: 1) Ditches A, A1, House 1 and a Pit 1 in Sector Southwest, and 2) a concentration of finds, part of a large area of rubbish deposition, in Square N35 in Sector North. Nova Nadezhda The site is situated on a low-lying terrace in the fertile floodplain of the Middle Maritsa Valley, c. 300 m from the river’s right bank, and covers an area of over 5 ha. It 1 consists of two low mounds, separated by a stream. In 2013-2014 rescue excavation for a railway construction took place on the north edge of the northwest mound in an area of 7500 m2. This part of the site was continually occupied from the beginning of the Early Neolithic (c. 6000 calBC) to the Final Chalcolithic (c. 4000 calBC), and then again in the Early Iron Age (11th–9th c. calBC). According to the radiocarbon dates, the Early Neolithic occupation started at c. 6000 BC and had a duration of c. 400 years (Table S3). Major archaeological features from this phase include five concentric ditches and at least one enclosure wall of wooden posts, as well as the remains of one burnt house1,2. The pottery samples are from the 2013 campaign and originate from the house (001) and from various locations in the fills of four enclosure ditches (003, 053, 054, 055). Serbia Blagotin Blagotin is situated in the northern outskirt of the village of Poljna near the foothills of the Blagotin Mountain. The site was discovered in 1984 during a survey of the region. Systematic excavations were conducted between 1989 and 2000 on a total area of c. 300 m2. Blagotin was a small settlement with a surface area of 0.5 ha, which provided important information about settlement life and architecture during the Early Neolithic, including seven semi- subterranean dwellings and numerous areas of rubbish deposition and pits. The 14C dates from this period of habitation have values around 6200-6000 calBC (Table S3). The site was reoccupied twice, during the Eneolithic (Baden-Kostolac culture) and the Early Iron Age (Hallstatt culture)3-6. All samples were selected from the 1995 excavations and originate from different stratigraphic levels and locations in the fill of Structure 3, a semi-subterranean dwelling. Divostin Discovered in 1952 during a survey led by the Archaeological Institute in Belgrade, the site of Divostin is considered as one of the major settlements of the Starčevo culture. The excavations took place in 1967 (by M. Bogdanovic) and between 1968-1974 (by D. Srejovic and A. McPherron) on a total area of c. 2,400 m2. Divostin was first inhabited by Starčevo groups (Divostin I). Five radiocarbon dates on wood charcoal from this period of habitation have values of c. 6000-5700 calBC (Table S3). From the Divostin I phase, semi-subterranean 2 huts and surface-level houses, fireplaces, hearths and ovens, rubbish pits and refuse areas were uncovered. Following a gap of a thousand years, the site was resettled by Late Vinča groups (Divostin II)7. The samples belonged to a pottery assemblage derived from the 1967 excavation of M. Bogdanovic of a pit of the Divostin I period in Sector D. Grivac The site is located in the locality of Barice near the modern village of Grivac. Habitation remains from the Early Neolithic (Grivac I-III) and Late Neolithic (Grivac IV-VI) were recorded at this site on a total area of c. 25 ha. Series of trenches were excavated in different parts of the site in 1953-1954, as well as in 1957, 1969, and 1989-1994. Features from the Early Neolithic phase included semi-subterranean and above-ground houses8. There is one radiocarbon date from this phase, from Trench B, with a value of c. 6200-6000 calBC (Table S3). The pottery samples originate from two separate areas: 1) from the west part of the site (Trench IV and V from 1957, Trench A and B from 1969 and Trench A from 1990); 2) from the east part (Trench 1954 and Trench A from 1994). Hungary Alsónyék The site of Alsónyék is located near the Danube in the southern part of the Sárköz region in the Tolna County, at the border of the Transdanubian Hills and the Great Hungarian Plain. The investigations at Alsónyék were conducted between 2006 and 2009 as a part of the rescue excavations along the M6 motorway on an area of c. 25 ha. The site was occupied during the Neolithic and Early Chalcolithic periods (Starčevo, LBK, Sopot and Lengyel culture groups). Alsónyék was the first large-scale excavation at a Starčevo site in Hungary and provided the largest Starčevo assemblage in this region so far. Occupation took place c. 5800-5730 cal BC (Table S3). The excavations identified two separate foci of Starčevo habitation, 10B and 5603. In the larger area 5603 some 500 features, mostly pits of various sizes and shapes, were assigned to the Starčevo period. Other Early Neolithic features included numerous well- preserved ovens, wells, and piles of burnt daub9,10. The samples originate from the fills of eleven pits in Area 5603, the main area of Starčevo occupation at Alsónyék. 3 Ecsegfalva 23 The site of Ecsegfalva was discovered in 1998 during large-scale archaeological surveys along the Kiri-Tó river. The excavations took place from 1999 to 2001 near an old meander of the Hortobágy-Berettyó river, northern tributary of Körös. Small-scale intensive excavations focused on three locations: Ecsegfalva 16, 18, and 23. A total of three trenches was opened at Ecsegfalva 23. The occupation of this location lasted for a short period of time with a starting date around 5800 calBC and peak activity between 5750 and 5650 cal BC (Table S3). Samples from the excavation campaigns in 1999-2001 were selected from all three trenches at Ecsegfalva 23: Area 23A (2 samples), Area 23B (31 samples) and 23C (8 samples). Supplementary Table S1 Description of samples containing significant concentrations of archaeological lipid residues (FA, fatty acids). 13 13 13 Site Lab Vessel Context type Lipids δ C16:0 δ C18:0 ∆ C Lipid Predominant number part and location classes (‰) (‰) (‰) concen- commodity tration (µg g-1) Yabalkovo YAB 05 Rim Pit fill, Pit 1 FA -27.2 -27.1 0.1 82 Non-Ruminant Adipose Fats YAB 07 Body Ditch fill, FA -27.3 -26.0 1.3 1356 Non-Ruminant A1/10 Adipose Fats YAB 11 Body Ditch fill, A/01 FA -27.4 -29.0 -1.6 89 Ruminant Adipose Fats YAB 22 Rim Concentration FA -28.0 -32.6 -4.6 54 Ruminant Dairy Fats of finds, N35 YAB 24 Base Concentration FA -28.8 -29.0 -0.2 49 Non-Ruminant of finds, N35 Adipose Fats YAB 27 Base Concentration FA -26.8 -31.8 -5.1 545 Ruminant Dairy Fats of finds, N35 YAB 30 Rim Concentration FA -25.1 -27.0 -2.0 151 Ruminant Adipose of finds, N35 Fats YAB 31 Rim Concentration FA -27.7 -31.4 -3.7 765 Ruminant Dairy Fats of finds, N35 YAB 32 Body Concentration FA -26.8 -28.1 -1.3 2632 Ruminant Adipose of finds, N35 Fats YAB 33 Body Concentration FA -26.1 -28.9 -2.8 3843 Ruminant Adipose of finds, N35 Fats YAB 35 Rim Concentration FA -24.8 -27.6 -2.8 266 Ruminant Adipose of finds, N35 Fats YAB 37 Base Concentration
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