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N6AMT1 Rabbit pAb

Catalog No.: A7201

Basic Information Background

Catalog No. This encodes an N(6)-adenine-specific DNA methyltransferase. The encoded A7201 enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite Observed MW monomethylarsonous acid to the less toxic dimethylarsonic acid. pf 23KDa this gene results in multiple transcript variants. A related has been identified on 11. Calculated MW 19kDa/22kDa

Category Primary antibody

Applications WB, IHC, IF

Cross-Reactivity Human, Mouse, Rat

Recommended Dilutions Immunogen Information

WB 1:500 - 1:2000 Gene ID Swiss Prot 29104 Q9Y5N5 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3).

Synonyms N6AMT1;C21orf127;HEMK2;MTQ2;N6AMT;PRED28;m.HsaHemK2P

Contact Product Information

www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification

Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data

Western blot analysis of extracts of Rat kidney, using N6AMT1 antibody (A7201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of PC-12 embedded rat brain using N6AMT1 embedded human kidney using N6AMT1 cells using N6AMT1 Rabbit pAb (A7201) at Antibody (A7201) at dilution of 1:100 (40x Antibody (A7201) at dilution of 1:100 (40x dilution of 1:100 (40x lens). Blue: DAPI for lens). lens). nuclear staining.

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