N6AMT1 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science N6AMT1 Rabbit pAb Catalog No.: A7201 Basic Information Background Catalog No. This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded A7201 enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite Observed MW monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf 23KDa this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. Calculated MW 19kDa/22kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 29104 Q9Y5N5 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3). Synonyms N6AMT1;C21orf127;HEMK2;MTQ2;N6AMT;PRED28;m.HsaHemK2P Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of Rat kidney, using N6AMT1 antibody (A7201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of PC-12 embedded rat brain using N6AMT1 embedded human kidney using N6AMT1 cells using N6AMT1 Rabbit pAb (A7201) at Antibody (A7201) at dilution of 1:100 (40x Antibody (A7201) at dilution of 1:100 (40x dilution of 1:100 (40x lens). Blue: DAPI for lens). lens). nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Noelia Díaz Blanco
Effects of environmental factors on the gonadal transcriptome of European sea bass (Dicentrarchus labrax), juvenile growth and sex ratios Noelia Díaz Blanco Ph.D. thesis 2014 Submitted in partial fulfillment of the requirements for the Ph.D. degree from the Universitat Pompeu Fabra (UPF). This work has been carried out at the Group of Biology of Reproduction (GBR), at the Department of Renewable Marine Resources of the Institute of Marine Sciences (ICM-CSIC). Thesis supervisor: Dr. Francesc Piferrer Professor d’Investigació Institut de Ciències del Mar (ICM-CSIC) i ii A mis padres A Xavi iii iv Acknowledgements This thesis has been made possible by the support of many people who in one way or another, many times unknowingly, gave me the strength to overcome this "long and winding road". First of all, I would like to thank my supervisor, Dr. Francesc Piferrer, for his patience, guidance and wise advice throughout all this Ph.D. experience. But above all, for the trust he placed on me almost seven years ago when he offered me the opportunity to be part of his team. Thanks also for teaching me how to question always everything, for sharing with me your enthusiasm for science and for giving me the opportunity of learning from you by participating in many projects, collaborations and scientific meetings. I am also thankful to my colleagues (former and present Group of Biology of Reproduction members) for your support and encouragement throughout this journey. To the “exGBRs”, thanks for helping me with my first steps into this world. Working as an undergrad with you Dr.
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N6AMT1 / HEMK2 (1-214, His-Tag) Human Protein – AR51691PU-N
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for AR51691PU-N N6AMT1 / HEMK2 (1-214, His-tag) Human Protein Product data: Product Type: Recombinant Proteins Description: N6AMT1 / HEMK2 (1-214, His-tag) human recombinant protein, 0.1 mg Species: Human Expression Host: E. coli Tag: His-tag Predicted MW: 25.3 kDa Concentration: lot specific Purity: >85% by SDS - PAGE Buffer: Presentation State: Purified Buffer System: Liquid, In Phosphate buffered saline (pH7.4) containing 20% glycerol, 1mM DTT. Protein Description: Recombinant human N6ATM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing. Stability: Shelf life: one year from despatch. RefSeq: NP_037372 Locus ID: 29104 UniProt ID: Q9Y5N5 Cytogenetics: 21q21.3 Synonyms: C21orf127; HEMK2; KMT9; m.HsaHemK2P; MTQ2; N6AMT; PRED28; PrmC Summary: This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Jul 2014] Protein Families: Druggable Genome This product is to be used for laboratory only.
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N6-Methyladenine DNA Modification in Human Genome
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