Leader in Biomolecular Solutions for Life Science N6AMT1 Rabbit pAb Catalog No.: A7201 Basic Information Background Catalog No. This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded A7201 enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite Observed MW monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf 23KDa this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. Calculated MW 19kDa/22kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 29104 Q9Y5N5 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-186 of human N6AMT1 (NP_877426.3). Synonyms N6AMT1;C21orf127;HEMK2;MTQ2;N6AMT;PRED28;m.HsaHemK2P Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of Rat kidney, using N6AMT1 antibody (A7201) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunofluorescence analysis of PC-12 embedded rat brain using N6AMT1 embedded human kidney using N6AMT1 cells using N6AMT1 Rabbit pAb (A7201) at Antibody (A7201) at dilution of 1:100 (40x Antibody (A7201) at dilution of 1:100 (40x dilution of 1:100 (40x lens). Blue: DAPI for lens). lens). nuclear staining. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..