Egyptian Journal of Aquatic Biology & Fisheries Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt. ISSN 1110 – 6131 Vol. 24(3): 333 – 349 (2020) www.ejabf.journals.ekb.eg DNA barcoding and comparative genetic diversification among species of family Sparidae in the coastal waters of Egypt 1 1 2 1 1 Nermin Ibrahim , Eman M. Abbas *, Ayman El-Seedy , Taha Soliman , Fawzia S. Ali 1Laboratory of Genetics, Aquaculture Division, National Institute of Oceanography and Fisheries (NIOF), Cairo, Egypt 13621. 2 Laboratory of cellular and Molecular Genetics, Department of Genetics, Faculty of Agriculture, Alexandria University, Alexandria, Egypt *Correspondence author:
[email protected] ARTICLE INFO ABSTRACT Article History: Species of family Sparidae, commonly called, sea breams, are widely distributed Received: April 13, 2020 from temperate to tropical waters and are of great economic interest. However, in Accepted: April 29, 2020 Egypt, limited data is available on genetic variation and evolutionary relationships of Online: May 2020 family Sparidae. Therefore, the study of the genetic diversity among sparid species is _______________ crucial for proper management and convenient strategies. The aim of this study was to evaluate the genetic diversification among 22 species belonging to family Sparidae Keywords: from the Mediterranean Sea and the Red Sea in Egypt using three different molecular Sparidae, markers. DNA barcoding, using Cyt-b mitochondrial gene, was applied as an initial Genetic diversity, step for species identification and diversification. In addition, Randomly Amplified RAPD, Polymorphic DNA (RAPD), and Inter Simple Sequence Repeat (ISSR) markers were ISSR, employed in estimating the genetic diversity among the studied species. Based on Mitochondrial Cyt-b, each molecular marker, a phylogenetic tree was constructed for the studied sparid Red Sea, species according to the calculated genetic distance/ similarity.