Glucanoacetobacter

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GLUCANOACETOBACTER INTRODUCTION Glucanoacetobacter is a genuine in the phylum proto bacteria. It is like rod shape and circular ends. It can be classified as gram negative bacterium. The bacterium is known for stimulating plant growth and being tolerant to acetic acid with one to three lateral flagella and known to be found on sugar cane. Gluconacetobacter diazotrophicus was discovered in Brazil by Bladimir A Cavalcante and Johannna/Dobereiner. Domain: Bacteria Phylum: Proteobacteria Class :Alphaproteobacteria Order : Rhodospirillales Family: Acetobacteraceae Genus:Gluconacetobacter Species:G.diazotrophicus CHARACTERISTICS Originally found in Alagoas, Brazil, Gluconacetobacter diazotrophicus is a bacterium that has several interesting features and aspects which are important to note. The bacterium was first discovered by Vladimir A. Cavalcante and Johanna Dobereiner while analyzing sugarcane in Brazil. Gluconacetobacter diazotrophicus is a part of the Acetobacteraceae family and started out with the name, Saccharibacter nitrocaptans, however, the bacterium is renamed as Acetobacter diazotrophicus because the bacterium is found to belong with bacteria that are able to tolerate acetic acid. Again, the bacterium’s name was changed to Gluconacetobacter diazotrophicus when its taxonomic position was resolved using 16s ribosomal RNA analysis. In addition to being a part of the Acetobacter family, Gluconacetobacter azotrophicus belongs to the Proteobacteria phylum, the Alphaprotebacteria class, and the Gluconacetobacter genus while being a part of the Rhodosprillales order. Other nitrogen-fixing species in this same genus include Gluconacetobacter azotocaptans and Gluconacetobacter johannae. Gluconacetobacter diazotrophicus cells are shaped like rods, have ends that are circular or round, and have anywhere from one to three flagella that are lateral. Based on these descriptions of the cell, Gluconacetobacter diazotrophicus can be classified with the bacillus genus. The cells be viewed as darkish brown or orange colonies under a microscope addition, the cells are aerobic which describes their need of oxygen. Because the bacterium is found on sugar cane when first discovered, as explained prior, and reacts strongly to high amounts of sugar, Gram Negative is the correct classification for Gluconacetobacter diazotrophicus. In addition to sugarcane, G. diazotrophicus has been found in different plants like coffee tree and pineapple. Gluconacetobacter diazotrophicus is also known for nitrogen fixation. .

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Protein Expression Profile of Gluconacetobacter Diazotrophicus PAL5, a Sugarcane Endophytic Plant Growth-Promoting Bacterium
Proteomics 2008, 8, 1631–1644 DOI 10.1002/pmic.200700912 1631 RESEARCH ARTICLE Protein expression profile of Gluconacetobacter diazotrophicus PAL5, a sugarcane endophytic plant growth-promoting bacterium Leticia M. S. Lery1, 2, Ana Coelho1, 3, Wanda M. A. von Kruger1, 2, Mayla S. M. Gonc¸alves1, 3, Marise F. Santos1, 4, Richard H. Valente1, 5, Eidy O. Santos1, 3, Surza L. G. Rocha1, 5, Jonas Perales1, 5, Gilberto B. Domont1, 4, Katia R. S. Teixeira1, 6 and Paulo M. Bisch1, 2 1 Rio de Janeiro Proteomics Network, Rio de Janeiro, Brazil 2 Unidade Multidisciplinar de Genômica, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil 3 Departamento de Genética, Instituto de Biologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil 4 Laboratório de Química de Proteínas, Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil 5 Laboratório de Toxinologia, Departamento de Fisiologia e Farmacodinâmica- Instituto Oswaldo Cruz- Fundac¸ão Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil 6 Laboratório de Genética e Bioquímica, Embrapa Agrobiologia, Seropédica, Brazil This is the first broad proteomic description of Gluconacetobacter diazotrophicus, an endophytic Received: September 25, 2007 bacterium, responsible for the major fraction of the atmospheric nitrogen fixed in sugarcane in Revised: December 18, 2007 tropical regions. Proteomic coverage of G. diazotrophicus PAL5 was obtained by two independent Accepted: December 19, 2007 approaches: 2-DE followed by MALDI-TOF or TOF-TOF MS and 1-DE followed by chromatog- raphy in a C18 column online coupled to an ESI-Q-TOF or ESI-IT mass spectrometer.
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